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All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) ANNALES BOGORIENSES Jurnal Perikanan Universitas Gadjah Mada Jurnal Medik Veteriner Wahana Peternakan Jurnal Kedokteran Hewan Policy Brief Pertanian, Kelautan, dan Biosains Tropika Jurnal Bioteknologi & Biosains Indonesia (JBBI) Annales Bogorienses Jurnal Bioteknologi & Biosains Indonesia
M Agus Setiadi
Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jl. Agathis, Kampus Dramaga IPB, Bogor, Jawa Barat, Indonesia 16880
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary

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IMPROVEMENT OF SHEEP OOCYTE COMPETENCE IN VITRO THROUGH GLUTATHIONE ADDITION Yusmala, Mitha; Supriatna, Iman; Setiadi, Mohamad Agus
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11512

Abstract

Oocyte competence is a primary factor for the success of embryo production in vitro. This research aims to determine the effect of supplementation glutathione in sheep oocytes’ maturation and fertilization rates in vitro. In the first research, maturation oocytes were conducted in a medium supplemented with GSH at graded doses for 24 hours. In the second research, 1.0 mM GSH (the best dose from research I) was supplemented in the maturation medium, fertilization medium, and their combination for 14 hours. The higher oocyte maturation rates were significant (P < 0.05) in the GSH supplementation of 1.0 mM (87.00%). Furthermore, supplementation of GSH on the maturation medium resulted in a higher normal fertilization rate (56.43%) than in other groups. It is concluded that GSH supplementation on the maturation medium is more effective in increasing the competence of sheep oocytes to be mature and results in improved fertilization rates.
Co-Authors . Hasbi Achmad Setiyono Adnin Adnan Agus Setiadi Agus Setiyono Alvien Nur Aini Amrozi Anak Agung Istri Sri Wiadnyani Ananda Ananda Anita Hafid Aras Prasetiyo Nugroho Arie Febretrisiana Arie Febretrisiana, Arie Arief Boediono Aries Boediono Ario Damar Asep Kurnia Asnath Maria Fuah, Asnath Maria Bambang Purwantara Bayu Rosadi Boenjamin Setiawan Cristovao, Adelaide Jose Pereira Dadang Jaenudin Dedi Rahmat Setiadi Dondin Sajuthi Ekayanti M. Kaiin Ekayanti M. Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Muyawati Kaiin Evy Damayanthi Faisal Amri Satrio Feni Dwi Kartika Gulo Frilianty Putri Frilianty Putri Gustina, Sri Hadi Sumarno Hafizuddin Hafizuddin Harry Murti Hasbi . Hasbi Hasbi Hasim Heri Sujoko I Ketut Mudite Adnyana Idqan Fahmi Iman Supriatna IPB, DGB Ita Djuwita Ita Djuwita Ita Djuwita ITA DJUWITA Kaiin, Ekayanti Mulyawati Kaiin, Ekayanti Mulyawati Ketut Adnyane Mudite Kurnia, Asep Kusdiantoro Mohamad La Ode Muhammad Aswad Salam Lala M Kolopaking Lisa Dwi Fannessia Lisa Praharani Luki Abdullah M Agil M Noordin M. Elmanaviean M. Haviz M. Khoeron . Ma'mun Sarma Masir, Ummul Masturi Muhajir Mitha Kurnia Sari Mohamad Fahrudin Mokhamad Fahrudin MOZES R. TOELIHERE MUHAMMAD AGIL Muhammad Imron Muhammad Imron Muhammad Imron MULYOTO PANGESTU Musthamin Balumbi Nahrowi Neta Fitria Yasa Ni Wayan Kurniani Karja Nofri Zayani Novi Suprihatin Nurbety Tarigan Nurkarimah, Dona Astari Nur’aisyah Amrah Safitri Okky Adi Bintara Oktariza, Wawan Praharani, Lisa Purwiyatno Hariyadi Rachmat Herman Rahmatullah Rahmatullah Rahmatullah Rahmatullah Reski Adelia Ridwan Affandi Rimas Prathita Agustin Rimayanti - Rizal Gusdinar Rizky Amrullah Chaniago Ronny Rachman Noor Sari, Mitha Kurnia Satya Gunawan Somanjaya, Rachmat Soni Sopiyana Sri Gustina Sri Purwaningsih, Sri SRI RAHAYU Sri Rahayu Srihadi Agungpriyono Sudradjat Sumiati Suria Darma Tarigan Suwandi, Syifa Damaianti Syafri Nanda Syahruddin Said TAKDIR SAILI Takdir Syahruddin Said Teguh Sumarsono Tuty L. Yusuf Tuty L. Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Ulfah Juniarti Siregar Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Widyasanti, Ni Wayan Helpina YULNAWATI YULNAWATI Yusmala, Mitha Zultinur Muttaqin