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Journal : Journal of Fisheries

DNA Barcoding of Red Algae (Rhodophyta) in Ternate Island Sea, North Maluku, Indonesia Achmad, Muhammad Janib; Akbar, Nebuchadnezzar; Ismail, Firdaut; Samman, Ardan; Subhan, Beginer; E Paembonan, Rustam; Arafat, Dondy
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i1.44436

Abstract

Abstract Ternate Island is located on the North Maluku Sea. The North Maluku Sea region includes the Wallacea area and the world's coral triangle. One of the organisms commonly found in this sea is red algae. Study aimed to determine the red algae species by phylogenetic tree analysis based on the rbcL gene as a DNA marker. The preserved red algae tissue samples were extracted with the Geneaid GP100 DNA Extraction Kit Plant. The DNA sample was amplified and then visualized by 1% agarose gel electrophoresis. The amplicon products were sequenced and then aligned with the rbcL gene database that was available at the NCBI gene bank. The phylogenetic tree was constructed using the UPGMA method. The results showed that red algae were identified into four species: namely Gibsmithia hawaiiensis (98.65%), C_rbcL sample was identical to Amansieae sp. (91.50%), D_rbcL sample was identical to Peyssonnelia sp. (95.54%), and G_rbcL sample was similar to Portieria hornemanniI (96.15%). Based on phylogenetic tree analysis, Gibsmithia hawaiiensis from North Maluku is closely related to species from Raja Ampat, West Papua, Indonesia, followed by the Philippines species. Special findings were found to carry out phylogenetic reconstructions that can answer inter-species kinship. The general finding is that the markers used can be used for phylogenetic construction. Phylogenetic construction of Peyssonnelia sp. in North Maluku is related to species from South Africa. North Maluku's Portieria hornemannii is closely related to a species from Korea. Highlight Research This study provides initial information about the DNA barcoding of red algae. Important for management purposes and determining the status of red algae in the future. This scientific information also provides an understanding of the relationships among red algae species in the world's oceans. Global warming has changed the temperature of seawater and affected the population of marine organisms.
Biodiversity and DNA Barcoding of Seagrass in Equatorial Latitudes Regional Representation of Tropical Indonesia Would Improve Clarity, Structure, and Scientific Precision Subur, Riyadi; Akbar, Nebuchadnezzar; Abubakar, Salim; Ismail, Firdaut; Paembonan, Rustam E; Subhan, Beginer; Arafat, Dondy; Irfan, Muhammad
Jurnal Ilmiah Perikanan dan Kelautan 2026: IN PRESS ISSUE (JUST ACCEPTED MANUSCRIPT, 2026)
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v18i2.83915

Abstract

Graphical Abstract Highlight Research 1. This research provides initial and current information regarding seagrass on small islands right on the equator. 2. directly depicts the diversity, density and cover of seagrass in a unique region, namely a small island crossed by the equator. 3. The environmental conditions of marine waters in seagrass areas on islands crossed by the equator have been described. 4. The latest information specifically describing seagrass and the ecology of equatorial marine environments. Essential for the development of future knowledge and technology. 5. DNA barcoding was capable of resolving field identification challenges and providing taxonomic clarification based on DNA sequence data.   Abstract The equator is a hypothetical line that separates the general region from the world.  Complete information on coastal ecosystems has not been provided for seagrass in the equatorial latitudes. Seagrass is dynamically distributed throughout the equatorial latitudes. The area that the equatorial latitudes also traverse is known as the indotropic region. Eastern Indonesia's Kayoa Island is situated directly on the equator.  A problem, namely how is the biodiversity of seagrass in the equatorial region. There are no published research on seagrass, particularly on biodiversity. The aim of this study is to reveal the diversity of seagrass in equatorial latitudes on the small islands. The methodology in this study was purposive sampling with a quadrant line transect was used to obtain the samples.  In situ identification of seagrass species was done.  Seagrass samples were taken from the leaves for DNA analysis purposes. Leaf samples were analyzed molecular in the laboratory. To determine the distribution of seagrass ecology and environmental were subjected to PCA analysis. The results found that six species of seagrass.  It has been discovered that seagrass may grow and develop in the tropical region under typical environmental conditions.  Despite the fact that six species were discovered, diversity was limited.  In this region, seagrass cover is typically high and supports a healthy species density.  In general concluded that diversity and density are low but cover is still quite good. DNA barcoding has revealed differences in morphological identification.This discovery represents preliminary data that can be investigated further in subsequent studies.    
Co-Authors Abdul Ajiz Siolimbona Abdul Motalib Angkotasan Abdurrachman Baksir Abdurrachman Baksir Abdurrachman Baksir Abdurrachman Baksir Abdurrachman Baksir Abdurrachman Baksir Abdurrachman Baksir Abdurrachman Baksir Abdurrachman Baksir Abdurrachman Baksir Abdurrachman Baksir Abdurrachman Baksir Abdurrachman Baksir Abdurrachman Baksir, Abdurrachman Abjan Ibrahim Abjan Ibrahim Abjan Ibrahim, Abjan Abubakar, Salim Abubakar, Yuyun Achmad, M Djanib Achmad, M Janib Achmad, M. Djanib Achmad, M. Janib Achmad, Muhammad Janib Aisyah, Siti Zanuba Al Hadad, M. Said Alfiansyah Kahar Andika Muhammad Antonius P Rumengan Anwar, Muhammad Fathur Ardan Samman Arfa Buamona Asep Sandra Budiman, Asep Sandra Ayu, Inna Puspa Baddu, S Baddu, Sartini Bahar Subur, Abd. Bashari, Muhammad Hasan Beginer Subhan Darmawaty Darmawaty Darmawaty, Darmawaty Darmiyati Muksin Daud, Kadri Dietriech Geoffrey Bengen Disnawati Disnawati, Disnawati Dondy Arafat Doni Nurdiansah E Paembonan, Rustam Eko S Wibowo Eko S Wibowo Eko S Wibowo Eko S Wibowo Eko S Wibowo Eko S Wibowo Eko Setyabudi Wibowo Eko Setyabudi Wibowo Elfahmi Elfahmi, Elfahmi Fadel, Ariyati H. Fardan S Ibrahim Fatgehipon, Sherra Nandami Fione Yalindua Firdaut Ismail Firdaut Ismail Firdaut Ismail Firdaut Ismail Firdaut Ismail Firdaut Ismail Firdaut Ismail Firdaut Ismail Firdaut Ismail Firdaut Ismail Firdaut Ismail Firdaut Ismail Hadad, M. Said Al Halikuddin Umasangadji Halikuddin Umasangaji Hapzi Ali Hari, Restu Fajar Hawis H Madduppa Hendrik A.W. Cappenberg Herawati Herawati Hi Abbas, M. Yunus Husen Rifai Huwae, Rikardo I WAYAN EKA DHARMAWAN I Wayan Nurjaya Ikbal Marus Ikbal Marus Ikbal Marus Ikbal Marus Ikbal Marus Ikbal Marus Ikbal Marus, Ikbal Inayah Inayah Inna Puspa Ayu Irfan Haji Irfan Haji Irfan Haji Irfan Haji Irfan Haji Irmalita Tahir Irmalita Tahir Irmalita Tahir Irmalita Tahir Irmalita Tahir Irmalita Tahir Irmalita Tahir Irmalita Tahir Irmalita Tahir Irmalita Tahir Irmalita Tahir Irmalita Tahir Irmalita Tahir Irmalita Tahir Irmalita Tahir Irmalita Tahir Irmalita Tahir Irmalita Tahir, Irmalita Ismail, Firdaut Iswandi Wahab Karman, Amirul Karman, Amirul Kepel, Rene C Kotta, Raismin Labenua, Rusmawati M Irfan M. Abjan Fabanyo M. Irfan Mantiri, Desy M. H Marenda Pandu Rizki Martini Djamhur Masykhur Abdul Kadir Masykhur Abdul Kadir Masykhur Abdul Kadir Masykhur Abdul Kadir, Masykhur Abdul Mesrawaty Sabar Mochtar Djabar Muhajirin Ahmad Muhajirin Ahmad Muhajirin Ahmad Muhammad Aris Muhammad Irfan Muhammad Irfan Muhammad Yunus Hi Abbas Mutmainnah Mutmainnah Mu’min Mu’min N Natih, Nyoman Metta N, Furqan Najamadidin, Najamuddin Najamuddin N Najamuddin Najamuddin, Najamuddin Nasir Haya Neviaty P Zamani Neviaty P Zamani NEVIATY PUTRI ZAMANI Ni Kadek Dita Cahyani, Ni Kadek Dita Novriyandi Hanif Nurdiansah, Doni Nyoman M N Natih Paembonan, Rustam E Paluphi, Raut Wahyuning R, Marenda Pandu Raden, Muhammad Sahlan R Rahimah, Insaniah Rahman Rahman Raismin Kotta Raismin Kotta Raismin Kotta Raismin Kotta Ramili, Yunita Rasidi, Rasidi Restu, Yunan Gilang Rikardo Huwae Rikardo Huwae Rikardo Huwae Rina Rina Rina Rina Riyadi Subur, Riyadi Rizqi, Marenda Pandu Rommy M. Abdullah Rommy M. Abdullah Rustam E Paembonan Rustam E Paembonan Rustam E Paembonan Rustam E Paembonan Rustam E Paembonan Rustam E Paembonan Rustam E Paembonan Rustam E Paembonan Rustam E Paembonan Rustam E Paembonan Rustam Effendi Rustam Effendi Rustam Effendi Paembonan Rustam Effendi Paembonan Rustam Effendi Paembonan Rustam Effendi Paembonan, Rustam Effendi S Baddu Sabar, Mesrawaty Sabaria Umalekhoa Salnuddin Salnuddin, Salnuddin Samad, Julkar Samria Abubakar Sani, Lalu M. Iqbal Sartini Baddu Serosero, Rugaya H Sidik, Marjanuddin A Simon I Patty Simon I Patty Simon I Patty Simon I Patty Simon I Patty Simon I Patty Simon I Patty Simon I. Patty Siolimbona, Abdul Ajiz Sunarti Suparto Abdullah Supyan Supyan Surahman Surahman Syafrizayanti, Syafrizayanti Taeran, Imran Wahab, Iswandi Waluyo Waluyo Wibowo, Eko S Wibowo, Eko Setyobudi Widhi, Raut Nugrahening Yadi D Naipon Yalindua, Fione Y Yidoatimojo, Sudibyo Yosie Andriani Yuyun Abubakar Zulham Apandy Harahap Zulhan A Harahap Zulhan A Harahap Zulhan A Harahap, Zulhan A Zulhan A. Harahap Zulhan Arifin Harahap