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<![CDATA[ACCURATE DETECTION OF VIRAL SEROTYPE DENGUE HEMORRHAGIC FEVER THROUGH AEDES SP MOSQUITOES USING REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION (RT-PCR) ]]>
<![CDATA[Erlin Yustin Tatontos]]>;
<![CDATA[Fihiruddin Fihiruddin]]>;
<![CDATA[Nurul Inayati]]>
<![CDATA[ Jurnal Riset Kesehatan Vol 10, No 2 (2021): NOVEMBER 2021 ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Semarang ]]>
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DOI: 10.31983/jrk.v10i2.7706
<![CDATA[Dengue Hemorrhagic Fever (DHF) caused Dengue virus which transmitted through the bite of the Aedes sp. Detection of dengue virus serotypes is essential for epidemiology as well as potential disease pathogens. RT-PCR method was more effective in mosquitoes, because the virus contained in the mosquito body is passed on to the next generation (trans ovarian). The purpose of this research is to know the serotype of DHF virus accurately through vector mosquito using RT-PCR method in Mataram City, so that vector control, early diagnosis and management of DHF disease could be done quickly and precisely. This research was a laboratory explorative research with cross sectional design that determines serotype of DHF virus through vector mosquito using RT-PCR method in Mataram City. The population of this study were Aedes aegypti female mosquitoes from eggs. Collected from houses located in Community Health Center Mataram city worked area with high dengue cases: Pagesangan, Karang Taliwang, Karang Pule, Tanjung Karang, Mataram, and Selaparang especially around the house’s sufferers. The results showed that only the mosquito group from the worked area of the Mataram Community Health Center showed the ribbon image with the base pair in accordance with serotype 1 (DENGUE 1), the other negative.]]>
<![CDATA[Tea Bag Biji Alpukat (Persea Americana Mill) terhadap Kadar Gula Darah Tikus Putih (Rattus Norvegicus) ]]>
<![CDATA[Ida Eliza]]>;
<![CDATA[Erlin Yustin Tatontos]]>;
<![CDATA[Rohmi Rohmi]]>;
<![CDATA[Yunan Jiwintarum]]>
<![CDATA[ Quality : Jurnal Kesehatan Vol. 11 No. 2 (2017): Quality : Jurnal Kesehatan ]]>
Publisher : <![CDATA[Politeknik Kesehatan Kemenkes RI Jakarta I ]]>
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DOI: 10.36082/qjk.v11i2.66
<![CDATA[ABSTRACT Blood sugar levels are a very important factor for body activity because blood sugar is the main energy source whose metabolism is liver. High blood sugar levels are called hyperglycemic conditions. The hyperglycemic state is a symptom of diabetes mellitus. Diabetes mellitus is a problem that is often found in Indonesian society. This disease can cause microvascular and macrovascular complications. Giving traditional medicine such as avocado seeds (P. americana Mill) in the form of tea bag is expected to reduce the level of blood sugar. Objective: To find out the effect of avocado seeds (P. americana Mill.) In the form of tea bag on blood sugar levels of white mice (R. norvegicus) wistar strains that are hyperglycemic Method: This study is a Pre-experiment research with a Pretest-One Group research design Posttes, using 6 rats aged two to four months. After a week of adaptation and successfully induced with alloxan, a pretest blood sugar level was tested at the time. The treatment was given for one week and posttest blood glucose levels were examined. Results: The study showed a mean blood sugar level of hyperglycemic wistar white rats before being given avocado seeds (P. americana Mill.) In the form of tea bag 226.4 and average after being given avocado seeds (P. americana Mill.) In the form tea bag is 154 mg / dL. Conclusion: Avocado seeds (P. americana Mill) in the form of tea bag have an influence on the decrease in blood sugar levels of white rats (R. norvegicus) hyperglycemic wistar strains Keywords: Avocado Seeds, Hyperglycemic, Blood Sugar Levels, Tea Bag]]>
<![CDATA[THE EFFECT OF MELON SEEDS FLOUR (Cucumis Melo L.) TOWARD TOTAL CHOLESTEROL LEVEL IN WHITE MALE WISTAR STRAIN RATS (Rattus Norvegicus) ]]>
<![CDATA[Maruni Wiwin Diarti]]>;
<![CDATA[Erlin Yustin Tatontos]]>;
<![CDATA[Yunan Jiwintarum]]>;
<![CDATA[Anggun Sari Mianti]]>
<![CDATA[ Jurnal Kesehatan Prima Vol 12, No 2 (2018): Jurnal Kesehatan Prima ]]>
Publisher : <![CDATA[poltekkes kemenkes mataram ]]>
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DOI: 10.32807/jkp.v12i2.188
<![CDATA[Abstract: Cholesterol is an important element in the body, but in high quantities, it can cause atherosclerosis that will eventually possess an impact on coronary heart disease. One of the foods that can reduce cholesterol is a food contains flavonoids which is found in melon seeds (Cucumis melo l.). It contains high fiber that can reduce either of excessive insulin or blood fat levels. The objective study was to determine the effect of Melon seeds flour Toward Total Cholesterol Level in White Male Wistar Strain Rats. This research was carried out with pre-expriment research design, used One group pretest-postest with 5 male wistar strain rats. Data collected in the form of data examination results of total cholesterol level before and after introducing Melon seeds flour. The mean result of total cholesterol test in white male rats before giving Melon seeds flour was 261 mg / dL, whereas the result of total cholesterol test after giving Melon seeds flour was 190.2 mg/dL. Cholesterol levels decreased by 70.8 mg/dl. In short, presenting Melon seeds flour (Cucumis melo l.) for 14 days can decrease total cholesterol level in white male wistar rats. Keywords: Melon Seeds, wistar strain, total cholesterol.]]>
<![CDATA[THE IMMUNE RESPONSE IN WORM INFECTION AT PUSKESMAS TANJUNG KARANG IN MATARAM ]]>
<![CDATA[Gunarti Gunarti]]>;
<![CDATA[Erlin Yustin Tatontos]]>;
<![CDATA[Urip Urip]]>
<![CDATA[ Jurnal Kesehatan Prima Vol 12, No 2 (2018): Jurnal Kesehatan Prima ]]>
Publisher : <![CDATA[poltekkes kemenkes mataram ]]>
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DOI: 10.32807/jkp.v12i2.189
<![CDATA[Abstrac: A worm infection can decrease sufferers’ health condition, nutrition, intelligence and their productivity thus, it can cause numerous of disadvantages economically. The immune response to worm infection begins with Th2 activation with a significant increase of IL-4, IL-5, IL-9, IL-10 and IL-13. The cytokines IL-5 that formed stimulates the growth and activation of eosinophils. The purpose of this research was to know the immune response by increasing the number of eosinophils to worm infection rate at Puskesmas Tanjung Karang. The research design was expost facto with cross sectional or transversal. The sample was communities’ faces and blood at Puskesmas Tanjung Karang as many as 50 people. The technique sampling was random from family members who have worm risk factors (poor sanitation conditions, living near animal cage, ground floor houses, pets such as dogs and cats). The correlation analysis of worm infection with eosinophils used Mann Whitney Test. The results showed that the prevalence of worm infection belong to mild category for Trichurin trichura was 10%, and the overall worm infection was 12% whereas the average number of eosinophils in the normal category was between 50-300 cells / mm². The results of statistical analysis were not significant (Asymp. Sig. 0.059) which means that there was no effect of the immune response with the number of eosinophils on the level of worminfection at Puskesmas Tanjung Karang.Keywords: Immune response, eosinophil, worm infection.]]>
<![CDATA[THE ALTERNATIVE DILUTE SOLUTION OF NaCl 0.9% AT THE GIEMSA STAINING ON THE INVESTIGATION THE MORPHOLOGY OF SPERMATOZOA ]]>
<![CDATA[Maruni Wiwin Diarti]]>;
<![CDATA[Erlin Yustin Tatontos]]>;
<![CDATA[Aden Turmuji]]>
<![CDATA[ Jurnal Kesehatan Prima Vol 10, No 2 (2016): Jurnal Kesehatan Prima ]]>
Publisher : <![CDATA[poltekkes kemenkes mataram ]]>
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DOI: 10.32807/jkp.v10i2.22
<![CDATA[Abstract : The quality of staining, the morphology of spermatozoon applays The Giemsa dye, is strongly influenced by the type of Giemsa thinner. The terms of diluent that can be used is having a buffer property. The solution of NaCl 0.9 %, besides having buffer properties also has a cheaper price. The research is a descriptive study that aims to determine NaCl 0.9% as the alternative of diluting solution at Giemsa staining on morphological examination of spermatozoon. This study was conducted to compare the quality of spermatozoon morphology staining by using Giemsa which was diluted with 0.9% NaCl solution and the solution of Phosphate Buffer. The result of the study indicated The Giemsa staining with NaCl 0.9% solution, is obtained the outstanding spermatozoon morphology staining absorption was 71.625% and the poor spermatozoon morphology staining absorption was 28.375%. The results of study which was applied the Phosphate Buffer solution, was obtained the outstanding spermatozoon morphology staining absorption was 81.563% and the poor spermatozoon morphology staining absorption was 18.438%. The NaCL 0.9% can be used as an alternative dilute solution at Giemsa staining on morphological examination of spermatozoon.]]>
<![CDATA[EDUKASI IBU-IBU PKK UNTUK PENERAPAN PROTOKOL KESEHATAN COVID19 DAN PENGGUNAAN ANTISEPTIK ALAMI ]]>
<![CDATA[Erlin Yustin Tatontos]]>;
<![CDATA[Siti Zaetun]]>;
<![CDATA[Nurul Inayati]]>;
<![CDATA[Urip Urip]]>;
<![CDATA[IGAN Danuyanti]]>
<![CDATA[ Jurnal Pengabdian Masyarakat Sasambo Vol 3, No 2 (2022): Mei ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Mataram ]]>
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DOI: 10.32807/jpms.v3i2.897
<![CDATA[Coronavirus Disease 2019 (COVID-19) adalah penyakit menular yang disebabkan oleh Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Peningkatan jumlah kasus berlangsung cukup cepat, dan menyebar ke berbagai negara dalam waktu singkat. Untuk mencegah penularan Covid19 di seluruh desa maka pada Pengabdian Kepada Masyarakat dilakukan edukasi ibu-ibu PKK untuk penerapan Protokol Kesehatan dan penggunaan Antiseptik alami. Sasaran PKM adalah ibu-ibu PKK di desa Karang Bayan Kecamatan Lingsar Lombok Barat berjumlah 40 orang. Metode pelaksanaan PKM adalah penyuluhan dan simulasi penerapan Protokol Kesehatan Covid19 yaitu 3 M serta demonstrasi dan praktek pembuatan Antiseptik rebusan daun Sirih. Dalam pelaksanaan PKM dilakukan Pre Test dan Post test serta meliputi pengetahuan, pemahaman/ sikap dan perilaku. Observasi pada praktek pembutan antiseptik alami.. Analisis secara deskriptif. Hasil Pre test 70% ibu-ibu PKK memakai masker , Post Test 100%; Pre test mencuci tangan pakai sabun/ antiseptik 37,5% menjadi 100% pada Post Test; Pre test Menjaga jarak/ menjauhi kerumunan 50% menjadi 100% pada Post Test. Hasil observasi ibu-ibu PKK dapat mempraktekan pembuatan antiseptik rebusan daun Sirih.]]>
<![CDATA[Pengaruh Variasi Masa Pengobatan Obat Anti Tuberkulosis (OAT) terhadap Billirubin dalam Urine Penderita Tuberkulosis Metode Carik Celup ]]>
<![CDATA[Riska Novitasari]]>;
<![CDATA[Urip Urip]]>;
<![CDATA[Erlin Yustin Tatontos]]>;
<![CDATA[Aurira Thrisna Dwi Aprianti]]>
<![CDATA[ Bioscientist : Jurnal Ilmiah Biologi Vol 10, No 1 (2022): June ]]>
Publisher : <![CDATA[Department of Biology Education, FSTT, Mandalika University of Education, Indonesia. ]]>
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DOI: 10.33394/bioscientist.v10i1.5668
<![CDATA[Treatment of Tuberculosis using Anti Tuberculosis Drugs (OAT) has a hepatotoxic effect that can cause liver function disorders, one of which is bilirubin. Examination of bilirubin can use blood and urine. Qualitative and semi-quantitative examination of bilirubin in urine by diazo reaction using the dipstick or dipstick method is widely used because it is fast, more practical, and more sensitive. The purpose of this study was to determine the effect of variations in the treatment period of Anti Tuberculosis Drugs (OAT) on bilirubin in the urine of tuberculosis patients. This research is an analytical observational research. The sample in this study amounted to 20 people in the initial and follow-up treatment periods. Urine samples in patients with tuberculosis were examined for urine bilirubin levels using the dipstick method. The results showed that the level of bilirubin in the urine qualitatively in the early stages of treatment had a positive value, namely 5 out of 9 tuberculosis patients (56%) with a positive level of 1 (+1) semi-quantitatively. The level of bilirubin in the urine qualitatively in the advanced stage of treatment has a positive value, namely 1 out of 11 tuberculosis patients (9%) with a positive level of 1 (+1) semi-quantitatively. The results of the Chi-Square bilirubin test in urine qualitatively and semi-quantitatively showed the value of p = 0.024 < = 0.05. The conclusion is that variations in the treatment period of Anti Tuberculosis Drugs (OAT) have an effect on bilirubin in the urine of tuberculosis patients.]]>
<![CDATA[Potensi Tepung Biji Kecipir (Psophocarpus tetragonolobus) sebagai Bahan Alternatif Sumber Nitrogen dalam Media Mannitol Salt Agar (MSA) untuk Pertumbuhan Staphylococcus aureus ]]>
<![CDATA[Urip Urip]]>;
<![CDATA[Nanda Yuniza Pratiwi]]>;
<![CDATA[Erlin Yustin Tatontos]]>;
<![CDATA[Maruni Wiwin Diarti]]>
<![CDATA[ Bioscientist : Jurnal Ilmiah Biologi Vol 10, No 2 (2022): December ]]>
Publisher : <![CDATA[Department of Biology Education, FSTT, Mandalika University of Education, Indonesia. ]]>
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DOI: 10.33394/bioscientist.v10i2.6138
<![CDATA[The purpose of this study was to determine the potential of winged bean (Psophocarpus tetragonolobus) seed powder as an alternative medium for Mannitol Salt Agar (MSA) against the growth of Staphylococcus aureus bacteria. This research method is true experiment with Completely Randomized Design (CRD). The experimental unit of this research was winged bean seed flour and Staphylococcus aureus. Winged bean seed flour used with 5 treatments, namely 2 gr, 4 gr, 6 gr, 8 gr, and 10 gr with each replication 5 times. The results showed that the standard MSA as a control showed an average of 3211 colonies, the average colony at a concentration of 2% was 372 colonies, the average colony on a 4% concentration plate was 419 colonies, the average colony on a 6% concentration plate was 544 colonies, the average colony on an 8% concentration plate were 622 colonies, and the average colony on a 10% concentration plate was 816 colonies. The conclusion of this study is winged bean seed powder has the potential as an alternative source of nitrogen in Mannitol Salt Agar (MSA) media for the growth of Staphylococcus aureus.]]>
<![CDATA[The Effectiveness of the Combination of Lethal Ovitrap Attractant of Local Organic Materials and Natural Larvicides in Dengue Hemorrhagic Fever Endemic Areas ]]>
<![CDATA[Erlin Yustin Tatontos]]>;
<![CDATA[I Wayan Getas]]>;
<![CDATA[Urip Urip]]>
<![CDATA[ Jurnal Penelitian Pendidikan IPA Vol. 8 No. 6 (2022): December ]]>
Publisher : <![CDATA[Postgraduate, University of Mataram ]]>
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DOI: 10.29303/jppipa.v8i6.2448
<![CDATA[Dengue fever is a viral infection that is transmitted to humans through the bite of an infected mosquito. Dengue prevention and control depends on effective vector control measures, including lethalovitrap.The purpose of this study was to determine the effectiveness of mango leaf infusions (Mangifera indica), rambutan leaves (Nephelium lappaceum) and cashew leaf (Anacardium ocidentale) in lethal ovitrap a combination of ethanol extract of rambutan leaves (Nephelium lappaceum), srikaya seed water extract (Annona squomosa) and Baccilus thuringiensis var israelensis (Bti) in the endemic area of Mataram City Dengue Fever, This study was an experimental study that tested and analyzed the effectiveness of nine lethal ovitrap atractant combination. The population and sample of this study were Aedes aegypti mosquitoes female from colonization from endemic areas of DHF in Mataram City. The laboratory test results from nine effective lethal ovitrap combination attractants were Cashew leaf infusion and Rambutan leaf ethanol extract with trapped eggs of Aedes aegypti mosquitoes averaging 133 grains and no eggs hatching into larvae. Statistical analysis with ANOVA followed by the Tukey HSD post-Hoc test with a significant difference of <0.05 the effectiveness of the combination of lethal ovitrap attractants, especially Cashew leaf infusion and ethanol extract from rambutan leaves and Rambutan leaf infusion and rambutan leaf ethanol extract. The results of the field test were the percentage of the number of eggs trapped in combination of Cashew leaf infusion (41.6%) and Rambutan leaf infusion with ethanol extract of Rambutan leaves (58.4%).]]>
<![CDATA[Bacterial Culture Streak Method for Inventory of Enterobacteriaceae Causes Diarrhea in the Body of House Fly (Musca Domestica) ]]>
<![CDATA[Urip Urip]]>;
<![CDATA[Titin Emilia Nirmala Sari]]>;
<![CDATA[Maruni Wiwin Diarti]]>;
<![CDATA[Erlin Yustin Tatontos]]>
<![CDATA[ Jurnal Penelitian Pendidikan IPA Vol 9 No 1 (2023): January ]]>
Publisher : <![CDATA[Postgraduate, University of Mataram ]]>
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DOI: 10.29303/jppipa.v9i1.2513
<![CDATA[Diarrhea is an endemic disease in Indonesia and is a potential disease Extraordinary Events (KLB) which are often accompanied by death. Based on data on diarrhea cases at the Narmada Community Health Center in 2017 there were 1.679 cases of diarrhea from 11 villages in Narmada District. One of the causes of these cases of diarrhea is the presence of house flies as a vector carrying disease. The purpose of this study was to determine the bacterial culture of the streak method for inventory of Enterobacteriaceae of diarrhea in the body of the house fly (Musca domestica). This research is descriptive observational. The sample in this study were 30 Musca domestica which were captured in the Kramajaya village environment in Nutrienth Agar Plate (NAP) and Mac Conkey Agar (MCA) media as well as the INVIC MUTSI Biochemical Test media on bacterial colonies that needed further identification to determine species. The analysis of the results was carried out descriptively. The identification results were found in the family Enterobacteriaceae genus Enterobacter agglomerans in the body of the house fly (Musca domestica) samples taken from the landfill and the family Moraxellaceae family genus Acinetobacter baumannii taken from the kitchen and home terrace]]>
