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All Journal HAYATI Journal of Biosciences MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Jurnal Ilmu Pertanian Indonesia Jurnal Ilmu dan Teknologi Hasil Hutan Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Forum Pasca Sarjana Jurnal Sain Veteriner Biospecies ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Natur Indonesia Microbiology Indonesia AL KAUNIYAH Jurnal Penelitian Hutan dan Konservasi Alam Jurnal Matematika & Sains Jurnal Ilmu Kelautan Spermonde agriTECH BERITA BIOLOGI JURNAL BIOLOGI INDONESIA ZOO INDONESIA Jurnal Biodjati BIOEDUSCIENCE Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Floribunda Jurnal Ilmu Kefarmasian Indonesia Konservasi Hayati Zoo Indonesia Makara Journal of Science Jurnal Kedokteran Hewan Jurnal Kefarmasian Indonesia

TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN

Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor

Author-ID : 251065

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Veterinary Other

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Acanthophyllia deshayesiana (Michelin, 1850) Coral Species Is Not Synonym With Cynarina lacrymalis (Milne Edwards & Haime, 1848) Robba Fahrisy Darus; Neviaty Putri Zamani; Suharsono Suharsono; Dedy Duryadi Solihin
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 3 (2016): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Acanthophyllia deshayesiana has a different habitat with Cynarina lacrymalis in the nature, but they have same character on living forms, diameter, and height of corallite. Both of these species are considered synonym, thus it needs verification study to describe whether it is synonym species or not based on morphological data. Eleven descriptive characters and seven morphometric characters were used to verify the synonym species of these coral. Descriptive data were performed by scoring method, while morphometric data were obtained from morphometric. Morphometric data were analyzed by Correspondence Analysis of Principal Coordinates (CAP) and Agglomerative Hierarchical Cluster (AHC), while descriptive data were analyzed by UPGMA (Unweight Pair Group Method with Arithmetic Mean). The result showed that both of these coral cannot differentiate based on morphometric measurement. It can differentiate significantly based on descriptive characters, so both of these coral are not synonym. Keywords: synonym, morphometric, descriptive, Cynarina lacrymalis, Acanthophyllia deshayesiana

Genetic Profile Assessment of Giant Clam Genus Tridacna as a Basis for Resource Management at Wakatobi National Park Waters Muhammad Nur Findra; Isdradjad Setyobudiandi; Nurlisa Alias Butet; Dedy Duryadi Solihin
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 22, No 2 (2017): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Giant clam population has been decreased in a few years. Resource management requires information from various aspects, such as ecological, population, and other aspects. This study was aimed at assessing the genetic profile of Tridacna giant clam in Wakatobi National Park waters using Cytochrome oxidase subunit I (COI) genetic marker. Sample collection was conducted around the three main islands, i.e., Wangi-wangi, Kaledupa, and Tomia. Genetic analysis using COI gene may contribute in identifying giant clams up to the species level and showed the relationship among species. The research found 41 specific nucleotide sites for the clams. T. crocea, T. squamosa and T. maxima had 2, 15 and 24 sites, respectively. COI gene as a biological marker was able to separate groups of giant clam by species. Nucleotide variation of T. crocea from Wakatobi was the highest among other locations, so it could be used as a genetic source for translocation and domestication. Keywords: cytochrome oxidase subunit I, specific nucleotide, Tridacna, Wakatobi National Park

Isolation and Characterization of Simian Retrovirus Type D from Macaca fascicularis and M. nemestrina in Indonesia DIAH ISKANDRIATI; UUS SAEPULOH; SILMI MARIYA; RICHARD F GRANT; DEDY DURYADI SOLIHIN; DONDIN SAJUTHI; JOKO PAMUNGKAS
Microbiology Indonesia Vol. 4 No. 3 (2010): December 2010
Publisher : Indonesian Society for microbiology

Simian type D retroviruses (SRVs) are one of the causative agents of simian acquired immunodeficiency syndrome (AIDS) in Asian macaques. In the past, SRV isolates from macaques had only been identified at the US primate centers, outside the country of origin and after the animals had been introduced into a new environment. In this study, we report the first isolation, cultivation and molecular characterization of the type D simian retrovirus naturally infecting wild caught macaques in their natural habitats in the country of origin, in this case, Indonesia. When peripheral blood mononuclear cells (PBMC) from Macaca fascicularis (Mf) and M. nemestrina (Mn) were co-cultured with Raji human B-cell line, syncytia were observed microscopically and confirmed by immunofluoresence assay using antibody to SRV-2. Immunoblot analysis of purified Mf-ET1006 from cell culture supernatants demonstrated that the viral core and envelope proteins reacted with rabbit anti-SRV. Sequence analysis of Mf isolates in the viral envelope region revealed high homology to SRV-2 (94-96%). On the other hand, the homologies in the envelope region of Mn isolates were less than 80% to SRV-1, SRV-2, SRV-3 and Mf isolates. This study suggests that the isolate from Mn may be different from any other published SRV isolates.

Cloning and Expression of Serotype-2 Simian Betaretrovirus Reverse Transcriptase Gene Isolated from Indonesian Cynomolgus Monkey in Escherichia coli UUS SAEPULOH; DIAH ISKANDRIATI; FUNGKEY HOETAMA; SELA SEPTIMA MARIYA; DEDY DURYADI SOLIHIN; JOKO PAMUNGKAS; DONDIN SAJUTHI
Microbiology Indonesia Vol. 7 No. 2 (2013): June 2013
Publisher : Indonesian Society for microbiology

In this study, we isolated the simian betaretrovirus serotype-2 (SRV-2) reverse transcriptase (RT) gene from infected Indonesian cynomolgus monkey (Macaca fascicularis). The gene was then cloned in Escherichia coli expression system. The SRV-2 RT gene is located between nucleotides 3284-4925 in the polyprotein (Pol) region encodes 547 amino acids. Analysis of expression using SDS-PAGE and western blot techniques showed a specific band of 64.9 kDa, indicating SRV-2 RT recombinant enzyme. Purification of SRV-2 RT recombinant enzyme produced 312 μg mL-1 protein with 7.1 U μL-1 enzyme activities. Application of this recombinant enzyme in reverse transcription-PCR (RT-PCR) of β-globin and β-actin genes produced DNA fragments of 206 and 350 bp, indicating amplification of β-globin and β-actin genes, respectively. Therefore, the expressed SRV-2 RT enzyme was proven to be functional, although the activity was low.

Karakteristik Molekuler Kelelawar (Microchiroptera), berdasarkan DNA Mitokondria (Gen COI) di Gua Sukabumi dan Sentul Jawa Barat Ismayati Afifah; Dedy Duryadi Solihin; Arzyana Sunkar
Al-Kauniyah: Jurnal Biologi Vol 14, No 1 (2021): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v14i1.14147

AbstrakCytochrome Oxidase I (COI) merupakan salah satu gen mitokondria untuk membantu konstruksi dari pohon filogeni yang dapat bertindak sebagai gen marker. Gen COI memiliki keakuratan dalam mengidentifikasi spesies dan umumnya digunakan sebagai “DNA Barcoding”. Informasi mengenai karakteristik genetik berdasarkan DNA mitokondria pada kelelawar di Sukabumi dan Sentul belum banyak dilaporkan. Tujuan dari penelitian ini untuk mengetahui keragaman genetik kelelawar berdasarkan DNA mitokondria dengan penanda Cytochrome Oxidase I (COI) sebagai DNA barcoding. Isolasi DNA total dilakukan menggunakan Kit Dneasy® Blood and Tissue Kit cat no 69504 (50) berdasarkan prosedur Spin-Column Protocol dengan modifikasi. Hasil penelitian ini menunjukkan bahwa gen COI telah berhasil mengidentifikasi karakteristik spesies. Dua haplotipe didapatkan dari masing-masing populasi. Berdasarkan barcode DNA menunjukkan populasi Sukabumi merupakan spesies Chaerephon plicatus dengan nilai identitas genetik sebesar 97,08%, sedangkan populasi Sentul menunjukkan perbedaan secara genetik dengan spesies Hipposideros larvatus dengan nilai identitas genetik sebesar 94,85%. Identifikasi secara genetik dengan menggunakan gen COI menunjukkan bahwa kelelawar yang berasal Sukabumi adalah spesies Chaerephon plicatus dengan jarak genetik sebesar 3,1%. Kelelawar yang berasal dari Sentul memiliki kedekatan dengan spesies Hipposideros larvatus namun memiliki jarak genetik sebesar 5,2%. AbstractCytochrome Oxidase I (COI) is one of the mitochondrial genes to help the construction of phylogeny trees that can act as marker genes. The COI gene has accuracy in identifying species and is commonly used as "DNA Barcoding". Information about genetic characteristics based on mitochondrial DNA in bats in Sukabumi and Sentul has not been widely reported. The purpose of this study was to determine the genetic diversity of bats based on Mitochondrial DNA with Cytochrome Oxidase I (COI) markers as DNA barcoding. Total DNA isolation was carried out using the Dneasy® Blood and Tissue Kit paint no. 69504 (50) based on the Spin-Column Protocol procedure with modifications. The results of this study indicate that the COI gene has successfully identified species characteristics. Two haplotypes were obtained from each population. Based on DNA barcodes, the population of Sukabumi is a species of Chaerephon plicatus with a genetic identity value of 97.08%, while the Sentul population shows genetic differences with the Hipposideros larvatus species with a genetic identity value of 94.85%. Genetic identification using the COI gene shows that the bats originating from Sukabumi is a spesies Chaerephon plicatus with a genetic distance of 3.1%. The bats originating from Sentul are closely related to the species Hipposideros larvatus but have a genetic distance of 5.2%.

VARIASI INTERSPESIFIK JULANG (AVES: BUCEROTIDAE) INDONESIA BERDASARKAN GEN CYTOCHROME-B DNA MITOKONDRIA Jarulis Jarulis; Dedy Duryadi Solihin; Ani Mardiastuti; Lilik Budi Prasetyo
BERITA BIOLOGI Vol 18, No 1 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i1.3714

Genetic information of Indonesian hornbills (Julang) is still limited. We sequenced the cytochrome b gene of three hornbill species (Rhyticeros undulatus, R. plicatus, and Aceros cassidix) to explore their genetic variation, distance, and phylogenetic. Nine blood samples for Cyt b from three hornbill species were isolated with Dneasy® Blood and Tissue Kit base on Spin-Column Protocol, Qiagen. SNP, genetic distance, and phylogeny were calculated by MEGA 6.0 software. This study was found variations in the Cyt b gene sequence (849 bp) between three species Indonesian Julang. Rhyticeros undulatus had the most SNP compared to R. plicatus and Aceros cassidix. Substitution of synonyms is more common than substitution of synonyms and transition substitution frequency tends to be much higher than transversion substitution. The three species of Indonesian Julang were markedly separate with an average genetic distance of 5.25%. and separated with the outgroup species at a genetic distance of 9.43%. Cytochrome b sequence genes from these three hornbill species are novel for identifying and classifying hornbills, and could be applied to prevent illegal poaching and conservation management in Indonesia.

KAJIAN KERAGAMAN GENETIK BURUNG KAKATUA TANIMBAR (Cacatua goffini, Finsch) MENGGUNAKAN PENCIRI "RAPD" Dwi Astuti; Siti N. Priyono; Heddy Julistiono; Dedy Duryadi
BERITA BIOLOGI Vol 4, No 2&3 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i2&3.1277

The study was conducted to analyse genetic diversity of Goffini Finsch Cockatoo (Cacatua goffini Finsch) bird using RAPD (Random Amplified Polymorphic DNA) marker.PCR (Polymerase Chain Reaction) was performed on DNA samples extracted from 14 birds,using 18 random 10-merprimers and 2 random 12-merprimers. Fourteen out of 20 primers (70 %) successfully amplified DNAfragmens and 11 out of 14 primers (78,57 %) generated 1-2 specific alkies.The result clearly demonstrated that the RAPD marker allows for genetic diversity analyses of these birds in efficiently. Tree of relationship among 14 birds showed that there were two groups in the population ofGoffin 's Cockatoo.

HABITAT DAN PERBEDAAN UKURAN TUBUH BURUNG KERAKBASI BESAR (Acrocephalus orientalis) PADA AWAL DAN AKHIR MASA MIGRASI DI INDONESIA Tri Haryoko; Dedy Duryadi Solihin; Dewi Malia Prawiradilaga
ZOO INDONESIA Vol 24, No 1 (2015): Juli 2015
Publisher : Masyarakat Zoologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52508/zi.v24i1.422

Acrocephalus Â orientalis termasuk burung migran pengunjung Â di Indonesia, yang berbiak di Asia Timur yaitu Siberia Selatan, Mongolia, Cina, Korea danÂ Jepang. Penelitian dilakukan di Danau Tempe (Sulawesi Selatan) dan Tanjung Burung (Tangerang, Banten) pada bulan Oktober- Desember 2008 dan Mei-Juli 2009. Penelitian ini bertujuan untuk : 1) menggambarkan tipeÂ habitat yang digunakan Acrocephalus orientalis 2) menjelaskan perbedaanÂ ukuran tubuh pada awal dan akhir masa migrasi di Indonesia.Â Hasil penelitian menunjukkan bahwa Danau Tempe dan Tanjung Burung merupakan wilayah di Indonesia yang menjadiÂ tempat singgah dan tujuan migrasi. Selama waktu penelitian sebanyak 256 ekorÂ burung Acrocephalus orientalis yang berkunjung pada kedua daerah tersebut berhasil ditangkap dan dilepaskan kembali. Jumlah burung yang tertangkap pada awal musim migrasi adalah 152 ekor dan pada akhir migrasi 104 ekor. Hasil Analysis of Variance (ANOVA) dengan SPSS 16.0 terhadap ukuran tubuh menunjukkan bahwa terdapat perbedaan nyata (P<0.05) Â antara burung pada awal dan akhir masa migrasi terhadap berat badan, panjang tarsus, panjang ekor, panjang total dan rentang sayap

PERBANDINGAN KARAKTER MERISTIK PADA Varanus salvator macromaculatus Deraniyagala, 1944 DARI POPULASI WILAYAH SUMATERA Sri Catur Setyawatiningsih; Evy Ayu Arida; Dedy Duryadi Solihin; Arief Boediono; Wasmen Manalu
ZOO INDONESIA Vol 24, No 2 (2015): Desember 2015
Publisher : Masyarakat Zoologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52508/zi.v24i2.680

Karakter meristik (hitung sisik) merupakan karakter pendiagnosa dalam mencandra biawak, termasuk Varanus salvator complex. V. s. macromaculatus tersebar paling luas di antara anak jenis Biawak Air sehingga diduga memiliki variasi morfologi. Hal tersebut ditunjukkan adanya ketumpangtindihan hitung sisik V.s. macromaculatus dengan anak jenis lainnya. Maka hitung sisik bukan sebagai karakter pendiagnosa yang mandiri. Oleh karenya digunakan pola warna sebagai karakter pendiagnosa lainnya. Penelitian ini bertujuan untuk membandingkan karakter hitung sisik dan pola warna tubuh V.s. macromaculatus asal Pulau Sumatera dan pulau-pulau satelitnya serta Pulau Jawa untuk melihat kekonsistenan dan kemandirian karakter meristik pada anak jenis tersebut. Kami juga mengidentifikasi karakter hitung sisik yang membedakan dua anak jenis, yaitu V.s. macromaculatus dan V.s. bivittatus. Karakter hitung sisik di bagian tengah tubuh (karakter S) dan pola warna tubuh dapat digunakan untuk membedakan populasi Biawak Air asal Pulau Simeulue dengan setiap populasi yang diteliti (populasi asal Pulau Sumatera, Pulau Batam, Pulau Kundur, dan Pulau Jawa). Karakter S bersifat konsisten dan bukan sebagai karakter pendiagnosa mandiri. Hitung sisik di sekitar pangkal ekor (karakter Q) dapat membedakan anak jenis Biawak Air, yaitu V.s. macromaculatus dan V.s. bivittatus dalam penelitian ini.

ETHOGRAM PERILAKU ALAMI INDIVIDU TIKUS SAWAH (Rattus argentiventer Robinson and Kloss, 1916) DALAM LABORATORIUM Agus Wahyana Anggara; Dedy Duryadi Solihin; Wasmen Manalu; Irzaman Irzaman
ZOO INDONESIA Vol 24, No 2 (2015): Desember 2015
Publisher : Masyarakat Zoologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52508/zi.v24i2.739

Perilaku merupakan respons senso-motorik makhluk hidup terhadap beragam stimulus dan fluktuasi kondisi lingkungan. Pengamatan terhadap perilaku alami tikus sawah telah dilakukan pada kondisi laboratorium. Tikus sawah tersebut ditangkap dari lapangan. Penelitian bertujuan untuk mengungkapkan dan mendeskripsikan perilaku alami tikus sawah. Semua aktivitas tikus percobaan dipantau kamera CCTV dan dilakukan pengamatan saksama untuk membuat ethogram. Hasil pengamatan menunjukkan bahwa aktivitas normal tikus sawah sepanjang periode aktifnya pada malam hari meliputi perilaku keluar-masuk lubang sarang, mengendus, mengawasi, menjelajah, makan dan minum, merawat diri, istirahat, dan menggali tanah. Sebagian besar aktivitas dilakukan pada pukul 17:30-22:00 WIB sehingga dapat dinyatakan bahwa periode tersebut merupakan waktu puncak aktivitas tikus sawah. Hasil percobaan diharapkan dapat menjadi standar perilaku alami tikus sawah untuk dibandingkan dengan respons tikus uji ketika dipaparkan kembali vokalisasi alaminya. Vokalisasi yang memberikan perbedaan respon perilaku tikus dianggap sebagai suara

Co-Authors Abdul Rahman Singkam Abdul Rahman Singkam Achmad - Taher ACHMAD FARAJALLAH Achmad Machmud Achmad Machmud Thohari Achmad Taher Achmad Taher Achmad, Taher Agus Nuryanto Agus Wahyana Anggara Agus Wahyana Anggara Agus Wahyana Anggara, Agus Wahyana Alam Putra Persada Ani Mardiastuti Ani Suryani Antonius Suwanto Any Aryani Ardi Kapahang Arief Boediono Arlyza1, Irma Shita Arzyana Sunkar Bahiyah Bahiyah Bambang Purwantara Cece Sumantri CECILIA ANNA SEUMAHU CECILIA ANNA SEUMAHU CHRISTIAN HANSJOACHIM SCHULZE D.D. Sastraatmadja DAMAYANTI BUCHORI Daniel Happy Putra Dedi . Soedharma Dedi Soedharma DEDI SOEDHARMA DEWI APRI ASTUTI Dewi Elfidasari Dewi Malia Prawiradilaga Dewi Malia Prawiradilaga, Dewi Malia Diah Iskandriati DIAH ISKANDRIATI DIETMAR BLOHM Dodi Nandika Dondin Sajuthi Dwi ASTUTI Dwi Astuti Dwi Sendi Priyono DYAH PERWITASARI -FARAJALLAH Epa Paujiah, Epa Evy Ayu Arida EVY AYU ARIDA Fahma Wijayanti Fahri Fahrudin, Fahri Faisal Mustafa Findra, Muhammad Nur FUNGKEY HOETAMA Gita Kusuma Rahayu Hadi Allikodra Hadi S Alikodra Haerul, Andi HAJRIAL ASWIDINNOOR Handayani , Handayani Handayani Handayani Hari Prayogo Harini Nurcahya Mariandayani Heddy Julistiono HEDDY JULISTIONO Hermanu Triwidodo I Gusti Agung Arta Putra I WAYAN SUANA Ibnu Maryanto Iman Rusmana Indah Fajarwati, Indah Irma Shita Arlyza Irzaman, Irzaman Isdradjad Setyobudiandi Ismayati Afifah Jakaria Jakaria Jamhari Jamhari Jarulis Jarulis Jarulis Jarulis Jarulis Jarulis Jito Jito Jito Sugardjito Jusmaldi Jusmaldi Kadarwan Soewardi Khustina, Yenny Chusna Khustina, Yenny Chusna Kunio Watanabe Lia Aseptin Murdini Lilik Budi Lilik Budi Prasetyo LILIK BUDIPRASETYO Lucia Johana Lambey M F Rahardjo M. F. Rahardjo M. Zairin Junior Mahmud, Rois MARIA BINTANG MF Rahardjo Mustafa Sabri Nastiti Kusumorini NEVIATY PUTRI ZAMANI Niken Subekti Niken TM Pratiwi Nurlisa Alias Butet Pamungkas, Joko Prasetyo Prasetyo Priyono, Siti N. Puji Rianti Retno Damayanti Soejoedono RICHARD F GRANT RIDWAN AFFANDI Rini Widayanti Robba Fahrisy Darus Roedhy Poerwanto Roedy Poerwanto Roedy Poerwanto Roni Koneri Ronny Rachman Noor Roza Elvyra Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudi Afnan Rudi Tarumingkeng Rudy C Tarumingkeng Safrida Safrida Safrida Safrida Saroyo Saroyo SATA YOSHIDA SRIE RAHAYU SATRIYAS ILYAS SELA SEPTIMA MARIYA SILMI MARIYA Siti N. Priyono SJAFRIDA MANUWOTO Soaloon Sinaga Soaloon Sinaga Sobir Sobir Sobir Sobir Sobir Sobir Solihin Solihin Sri Catur Setyawatiningsih Sri Catur Setyawatiningsih sri murtini . SRI NINGSIH Sri Sulandari Sri Supraptini Mansjoer Subyakto Subyakto Sugardjito Sugardjito SUHARSONO Suharsono Suharsono Sulistiyani Sulistiyani SULISTIYANI SULISTIYANI SULISTIYANI SULISTIYANI Sulistiyani, Sulistiyani Surjono Surjokusumo Syafyudin Yusuf Syaiful Anwar Syamsul Bachry Taher, Achmad Tedjo Sukmono Thohari Thohari Tike Sartika Tri Haryoko Tri Haryoko, Tri Tutik Wresdiyati UUS SAEPULOH UUS SAEPULOH Wasmen Manalu Yuli Wahyu Tri Mulyani YULIN LESTARI Yuni Cahya Endrawati Yus Rusila Noor Yus Rusila Noor Yusnarti Yus

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