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Carbohydrate is a diverse compound in the body and can be as glycoconjugate which bound to protein in proteoglycan and glycoprotein shape, and bound to lipid in glycolipid shape as well. The study was conducted to get information image of pattern distribution of the glycoprotein at stomach gland from various age levels at Macaca fascicularis (Mf) after and before bearing. Gastric specimen was collected processed through microtechnique procedure, followed by histochemistry Lectin staining to det Erdiansyah Rahmi; Dondin Sajuthi; Srihadi Agungpriyono; Erni Sulistiawati
Jurnal Primatologi Indonesia Vol. 6 No. 2 (2009)
Publisher : Pusat Studi Satwa Primata LPPM-IPB

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Carbohydrate is a diverse compound in the body and can be as glycoconjugate which bound to protein in proteoglycan and glycoprotein shape, and bound to lipid in glycolipid shape as well. The study was conducted to get information image of pattern distribution of the glycoprotein at stomach gland from various age levels at Macaca fascicularis (Mf) after and before bearing. Gastric specimen was collected processed through microtechnique procedure, followed by histochemistry Lectin staining to detect glycoprotein from stomach. Observation result of given score subjectively into four categories: (-) none/negatif, (+) low, (++) medium,and (+++) height. The results obtained were tabulated and analyzed diskriptively. We found that glycoproteins complex exist in the glandullar stomach of long tailed macaques are mannose, glucose, galactose, N-acetil-D-glocosamine and N-acetil-D-galactose all of these glycoproteins complex was observed at age foetus 70 days. Glycoprotein can act as energy resource used for grouht and protections toward stomach. Keywords: glycoprotein, lectin, stomach gland, Macaca fascicularis

Uji Tuberculin pada Kulit Monyet Ekor Panjang (Macaca fascicularis): dalam Upaya Pencegahan Penyakit Zoonosis Tuberkulosis (TBC) di Kawasan Wisata Pulau Weh Sabang Erdiansyah Rahmi; Amalia Sutriana; Dwina Aliza
Jurnal Primatologi Indonesia Vol. 7 No. 1 (2010)
Publisher : Pusat Studi Satwa Primata LPPM-IPB

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Abstrak: Penelitian ini bertujuan untuk memperoleh informasi tentang penularan penyakit tuberkulosis (TBC) pada monyet ekor panjang (Macaca fascicularis) di kawasan wisata Pulau Weh, Sabang dan untuk mendukung program pemerintah dalam menghasilkan Indonesia bebas TBC. Penelitian ini dilakukan dalam beberapa tahap setelah memperoleh persetujuan dan izin dari Balai Besar Konservasi dan Sumberdaya Alam Aceh dan instansi terkait di Sabang. Tahapan berikutnya menentukan lokasi, populasi, dan identifikasi monyet ekor panjang serta menentukan jumlah monyet ekor panjang per kelompok. Pemeriksaan hewan dengan melakukan sedasi menggunakan ketamin (10mg/kg) dan xylazine (2 mg/kg) secara intramuskuler. Skrining menggunakan tes tuberkulin metode (mammalian old tuberculin) dilakukan terhadap semua hewan 0,1 mL (135000 Tuberkulin Unit) secara intradermal pada palpebra. Hasil uji tuberculin dibaca pada 24, 48, dan 72 jam pasca suntik. Pengujian diulang pada minggu kedua. Data ditabulasi dan dianalisis secara deskriptif. Dari identifikasi populasi monyet di dua wilayah itu ditemukan satu kelompok monyet pada kilometer nol (N 05 ° 54 '19,1 "E 95 ° 12' 57,4") dengan populasi 14 ekor, sedangkan di wilayah Cot Murong ( N 05 ° 54 '19,1 "E 95 ° 12' 57,4") ditemukan dua kelompok dengan populasi 12 dan 14 ekor. Skrining tuberkulosis menggunakan metode ini menunjukkan bahwa tidak ada insiden TBC pada monyet ekor panjang di kawasan wisata Sabang.Abstract: This research was purposed to obtain the information about the evidence of Tuberculosis (TB) infectious disease on long tail macaque (Macaca fascicularis) in tourism area Weh Island, Sabang and to support the government’s program in generating Indonesia free TB, as well. This research was conducted on many stages, those were permits arrangement with BKSDA Aceh and Forestry Officer Sabang, location identification of long tail macaque populations, and identification of the amount of long tail macaque population in each groups. The monkeys chosen were restrained and anaesthetized using Ketamin (10mg/kg) and xylazine (2mg/kg) intramuscularly. Screening test using tuberculin skin test (TST) method (old mammalian tuberculin kit) was carried out to all samples, 0.1 mL (135000 Tuberculin Unit) on palpebrae intradermally. The effect of TST was examined at 24, 48, and 72 hours post treatment. The test was repeated on the second week. The data were tabulated and analyzed descriptively. From the identification of macaca populations in two areas was found one group of macaca in Kilometer Nol (N 05° 54’ 19,1" E 95° 12’ 57,4") with the population of 14 macacas whereas in Cot Murong area (N 05° 54’ 19,1" E 95° 12’ 57,4") found two groups with the population of 12 and 14 macacas. TB screening test using TST method showed that there were no incidents of TB in long tail macaque in Sabang tourism area.Keywords : Tuberculin Skin Test, Sabang, Macaca fascicularis

Isolation of enterobacteriaceae bacteria species from feces of Sumatran Orangutans (Pongo abelii) . Darmawi; Erdiansyah Rahmi; Maryulia Dewi; Joharsyah Hutabarat; Mahdi Abrar; . Fakhrurrazi
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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The porpouse of this research aimed to isolate enterobacteriaceae bacteria from feces of Pongo abelii. The samples of feces were collected from 15 captive orangutans in Orangutan Sumatera Batu Mbelin Sibolangit Qurantine, North Sumatra. Of each sample was cultured in nutrient broth media using sterilecotton swabs or Pasteur pipettes, and incubated at 37°C temperature for 24 hours. Culture was spared on Methylene Blue Agar (Oxoid), examined by Gram staining, and tested by biochemically. The result showed that significantly more common appear Escherichia sp. (93,33%) and fewer Edwardsiella sp. (66,67%) wereisolated from feces samples of P. abelii. Others enterobacteriaceae found in feces of P. Abelii were Shigella sp. (46,67%), Klebsiella sp. (33,33%), Citrobacter sp., and Salmonella sp. (13,33%), respectively

In vitro antimalarial activity of Jaloh leaves extract on Plasmodium falciparum Nuzul Asmilia; Amalia Sutriana; Erdiansyah Rahmi; . Sugito
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
Publisher : Syiah Kuala University

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Malaria still cause health problem in tropics including Indonesia, since its generate high mortality rate. Medication of Malaria has been performed long time ago without optimum result. One reason is the high rate of P.falciparum resistance on conventional antimalaria drug. This research aim to evaluate the activity of jaloh leaves extract (Willow leaves) on the inhibition of P.falciparum in vitro.This was a laboratory experimental research that using Indonesian isolates of cloroquin resistance P.falciparum obtained from NAMRU-2, Jakarta. P.falciparum isolate that has been synchronized were divided in to 3 groups of treatment : Jaloh leaves ethanolic extract, jaloh leaves ethyl acetate extract, and jaloh leaves n-hexane extract. Each group were then alloted into 5 dosage levels: 6,25ug/ml; 12,50ug/ml; 25,00ug/ml; 50,00ug/ml; and 100ug/ml. The observation of parasitemia degree were done 48 hours post-incubation in which the thick blood smear were colored with Giemsa and then examined using bioculer microscope. Data were analyzed using one way ANOVA and Newman-Keuls test. The result showed that the percentage of P.falciparum growth inhibition were sinificantly different (P0.05) among treatments wherein ethanol extract and ethyl acetate extract more inhibit P.falciparum growth than the hexane extract. The administration of different dosage also resulted in significance different (P0.05) of P.falciparum growth inhibition. This research concludes that jaloh leaves extract could inhibit P.falciparum growth.

CARBOHYDRATES OF CHANGES DURING THE FOLLICULAR DEVELOPMENT IN THE OVARY OF THE MOUSE DEER, TRAGULUS JAVANICUS Hamny -; Srihadi Agungpriyono; Ita Djuwita; Chairun Nisa; Wahono Esthi Prasetyaningtyas; Erdiansyah Rahmi
Jurnal Veteriner Vol 9 No 1 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The data available on the female reproductive organ of mouse deer (Tragulus javanicus) is still very limited. A study was therefore conducted to investigate the distribution and the concentration of carbohydrate residues during the development of ovary follicles. An ovary at luteal phase was used in this study. Thin sections of the ovary were prepared occording to the standard methods and they were then histochemically stained with flourecnece-labelled lectins such as peanut agglutinin (PNA), Ricinus communis agglutinin (RCA), Concanavalin A (Con A), Winged bean agglutinin (WGA) and Ulex europaeus agglutinin (UEA). The result showed that changes in the distribution and the concentration of carbohydrate occured during the development of the follicle. During the preantral stage, the cytoplasm of oosit contained carbohydrate with the residues of glucosa dan mannosa. Zona pelusida contained carbohydrates with residues of glucosa, mannosa, galactosa dan N-asetylgalactosamine, whereas extracellular matrix contained carbohydrate with the residues of glucosa dan mannosa. In the antral follicle, the cyitoplasm of oocytes contained carbohydarte with the residues of galactosa dan N-asetylgalactosamine, whereas its zona pelusida, extracellular matrix and follicular fluid contained carbohydarte with the residues of fucosa, N-asetylglucosamin and cyalic acid. Diffrences in the types and the distribution pattern of carbohydrates were observed in this study, both in preantral and antral follicles.

HISTOLOGIS INSANG DAN LABIRIN IKAN GURAMI (Osphronemus gouramy Lac.) (Histological Gill and Arborencent of Carp (Osphronemus gouramy Lac.) Vina Veronica; cut dahlia iskandar; erdiansyah rahmi
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 1 (2017): NOVEMBER - JANUARI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Penelitian ini bertujuan untuk mengetahui histologis sistem respirasi ikan gurami (Osphronemus gouramy Lac.). Organ yang di ambil adalah insang dan labirin dari 2 ekor ikan gurami dan diteliti dengan metode histologis eksplorasi. Pengamatan histologis dilakukan setelah pembuatan preparat histologis yang diwarnai dengan hematosiklin eosin (HE). Hasil penelitian menunjukkan bahwa pada insang ikan gurami terdiri atas lengkung insang, tulang rawan penompang, lamela primer dan lamela sekunder. Pada lamela primer terdapat tulang rawan penompang, dan pembuluh darah, sedangkan pada lamela sekunder terdapat sel epitelium, sel pilar, sel mukus, sel klorida, dan pembuluh darah. Selanjutnya Labirin terdiri dari sel epitel pipih selapis, pembuluh darah, dan tulang rawan elastis yang tersusun atas kondrosit, kondroblas dan perikondrium sebagai pembungkus tulang rawan elastis.The Objective of the present study to determined the histological respiratory system of carp (Osphronemus gouramy Lac.). Arborencent and gill of the two carp was examinated by histological exploration. Histological observation was stained by hematoxylin eosin (HE). The study perfomed that the gills of carp consist of cartilage, primary lamella and secondary lamella. The primary lamella was showed cartilage and blood vessels, meanwhile the secondary lamella was perfomed epithelial cells, pillar cells, mucus cells, khlorida cells, and blood vessels. The Next arborecent was drawn layered flattened epitelial cells, blood vessels, elastic cartilage was wrapped chondrocytes, kondroblast and pericondrium as an elastic cartilage wrapping.

HISTOLOGIS INTESTINUM IKAN GURAMI (Osphronemus gouramy Lac.) PADA FASE BENIH DAN DEWASA (The Histological of Intestine of Carp (Osphronemus gouramy Lac.) at Seed and Adult Phase) Risfi Febrina Tri Utami Emha; cut dahlia iskandar; erdiansyah rahmi
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 2 (2018): FEBRUARI - APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

ABSTRAK Penelitian ini bertujuan mempelajari histologis intestinum ikan gurami (Osphronemus gouramy Lac.) pada fase benih dan dewasa. Saluran pencernaan yang diambil adalah intestinum yang berasal dari enam ekor ikan gurami. Sampel intestinum kemudian dibuat menjadi preparat histologis dengan pewarnaan hematoksilin eosin (HE) dan diamati menggunakan metode histologi eksplorasi. Hasil penelitian menunjukkan bahwa ikan gurami pada fase benih terbagi ke dalam 2 fase yakni fase fry dan fase fingerling. Pada fase fry bersifat karnivora, pada fase fingerling bersifat omnivora, dan pada fase dewasa ikan tersebut bersifat herbivora. Selanjutnya histologis intestinum ikan gurami pada fase fry, fase fingerling, maupun fase dewasa pada umumnya sama yakni tersusun atas empat lapisan, yaitu tunika mukosa, tunika submukosa, tunika muskularis, dan tunika serosa.Kata Kunci: histologi, intestinum, ikan gurami ABSTRACT The aims of the researchs was to determined the histological exploration of carp (Osphronemus gouramy Lac.) intestine in seed and adult phase. Histological samples stained with haematoxylin-eosin (HE) then observed using histological methods of exploration. The result revealed that carp in the seeds phase was divided into 2 phases namely fry and fingerling phase. In the fry phase was carnivorous, the fingerling phase was omnivorous, and in the adult phase the fish was herbivorous. The next histolgy of intestine of gouramy fish in the fry, fingerling and adults phase were consist of four layers, namely tunika mucosa, tunica submucosa, tunica muskularis, and tunica serosa.Keywords:histology, intestine, carp

PENGARUH LAMA WAKTU SEXING DENGAN METODE ELEKTRIK TERHADAP DAYA TAHAN HIDUP SPERMATOZOA SAPI ACEH YANG DISIMPAN PADA SUHU 5°C (The Effect of Long Time Sexing with Electrical Method on the Survival of Aceh Cow’s Spermatozoa Stored at a Temperature of 5°C) Anisa Diah Ulfa; Dasrul Dasrul; Dian Masyitha; Azhar Azhar; Erdiansyah Rahmi; Roslizawaty Roslizawaty
JURNAL ILMIAH MAHASISWA VETERINER Vol 3, No 2 (2019): FEBRUARI-APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

ABSTRAK Penelitian ini bertujuan mengetahui pengaruh lama waktu sexing dengan metode elektrik terhadap daya tahan hidup spermatozoa sapi aceh yang disimpan pada suhu 5°C. Penelitian ini menggunakan rancangan acak lengkap (RAL) pola satu arah. Semen ditampung dari 2 ekor pejantan menggunakan vagina buatan. Semen yang berkualitas baik dibagi ke dalam 3 kelompok perlakuan yaitu kelompok sexing dengan metode elektrik yang dialiri listrik 1,5 volt selama 3 menit (P1); 6 menit (P2); dan 10 menit (P3) yang selanjutnya disimpan dalam suhu 5°C. Tiap perlakuan diamati bagian anoda (P1a, P2a, P3a) dan katoda (P1k, P2k, P3k). Data yang diperoleh dianalisis dengan analysis of variance (ANOVA) pola satu arah yang dilanjutkan dengan uji Duncan untuk melihat perbedaan daya tahan hidup spermatozoa antar perlakuan, sedangkan untuk melihat daya tahan hidup spermatozoa antara bagian anoda dan katoda diuji dengan uji T. Hasil pengamatan daya tahan hidup spermatozoa setelah pendinginan pada bagian anoda secara berturut-turut 8,92 ± 0,74 jam; 7,00 ± 1,70 jam; 5,00 ± 1,00 jam, sedangkan bagian katoda secara berturut-turut 9,42 ± 1,16 jam; 8,33 ± 1,17 jam; 5,92 ± 1,11 jam. Lama waktu sexing dengan metode elektrik berpengaruh secara nyata (P0,05) terhadap daya tahan hidup spermatozoa. Waktu sexing selama 3 menit menghasilkan daya tahan hidup spermatozoa sapi aceh lebih lama dibandingkan waktu sexing 6 menit dan 10 menit selama penyimpanan pada suhu 5°C. Tidak ada perbedaan yang bermakna antara daya tahan hidup spermatozoa bagian anoda dengan katoda.Kata Kunci: spermatozoa, waktu sexing, metode elektrik, daya tahan hidup ABSTRACTThe study aims to determine the effect of long time sexing with electrical method on the survival of aceh cow’s spermatozoa stored at a temperature of 5°C. This study using one way completely randomized design. Semen was collected from 2 males, using artificial vagina. Good quality semen was divided into 3 groups; group was electrified with 1,5 volt during 3 minutes (P1); 6 minutes (P2); and 10 minutes (P3) and stored in temperature of 5°C. Each group observed the anode (P1a, P2a, P3a) and cathode (P1k, P2k, P3k). The data obtained was analyzed with the one way analysis of variance (ANOVA), followed by Duncan test to analyzed the viability of spermatozoa between groups, while to analyzed the viability of spermatozoa between the anode and the cathode used the T test. The result show the viability after cooling in groups anode were 8,92 ± 0,74 hours; 7,00 ± 1,70 hours; 5,00 ± 1,00 hours and in groups cathode were 9,42 ± 1,16 hours; 8,33 ± 1,17 hours; 5,92 ± 1,11 hours. Long time sexing with electrical method affects significantly (P0,05) sperm viability. Sexing time during 3 minutes showed the viability of aceh cow’s spermatozoa longer than sexing time during 6 minutes and 10 minutes stored at a temperature 5°C. There was no significant differences of the viability between the anode and the cathode.Key word: spermatozoa, sexing time, electrical method, viability

GAMBARAN HISTOLOGI SISTEM RESPIRASI IKAN GABUS (Channa striata) (Histological Respiratory System of Snakehead (Channa striata)) Sedrisa Lidya Pertiwi; Zainuddin Zainuddin; Erdiansyah Rahmi
JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 3 (2017): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

ABSTRAK Penelitian ini bertujuan mengetahui histologis sistem respirasi ikan gabus (Channa striata). Organ yang diambil adalah insang dan labirin yang berasal dari lima ekor ikan gabus yang diamati dengan metode histologi eksplorasi. Hasil penelitian menunjukkan bahwa insang terdiri dari lengkung insang dan filamen insang, lengkung insang tersusun atas gerigi insang (gill rakers), epitel mukosa, membran basal, submukosa, tulang, jaringan lemak, dan pembuluh darah. Filamen insang terdiri dari lamela primer dan lamela sekunder, lamela primer tersusun atas tulang rawan yang dibungkus oleh perikondrium, central venous sinus dan sel klorida, sedangkan pada lamela sekunder terdapat sel epitelium pipih, sel mukus, sel pilar dan eritrosit. Labirin terdiri dari tunika mukosa dan tunika submukosa.ABSTRACT This study aims to determine the histologic respiratory system (Channa striata). The organs that was taken are gills and labyrinth that comes from five snakehead, observed by histology method of exploration . The result showed that gill consists of gill’s arch and gill’s filament, gill’s arch consists of gill rakers, mucosa epithel, basal membrane, sub mucosa, bone, fat tissue, and vein. Gill’s filament consists of primary lamella and secondary lamella, primary lamella consists of cartilage that coated by pericondrium, central venous sinus, and chloride cells. Secondary lamella consists of epithelium cells, mucus cells, pillar cells, and eritrosit. Labyrinth consists of tunica mucosa and tunica submucosa.

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