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All Journal HAYATI Journal of Biosciences Media Veteriner Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Veteriner Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Jurnal Kedokteran Brawijaya The Journal of Experimental Life Sciences (JELS) Journal of Indonesian Tourism and Development Studies Biotropika Wacana, Jurnal Sosial dan Humaniora Journal of Innovation and Applied Technology Jurnal Pembangunan dan Alam Lestari Natural B AGRIVITA, Journal of Agricultural Science UNEJ e-Proceeding Berkala Kedokteran Jurnal Triton Berkala Penelitian Hayati Jurnal Kedokteran Hewan
Moch Sasmito Djati
Biology Department, Faculty Of Mathematics And Natural Science, Brawijaya University, Malang
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary Other

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Tapak Liman (Elephantopus scaber L.) Leaves Ethanol Extract Improves the Production of IL-6 and IL-17 Cytokines in Mice with Bleomycin-induced Pulmonary Fibrosis Nabilah, Sarah Nahdah; Christina, Yuyun Ika; Dwijayanti, Dinia Rizqi; Rifa’i, Muhaimin; Djati, Muhammad Sasmito
The Journal of Experimental Life Science Vol. 14 No. 1 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.01.02

Abstract

IL-6 and IL-17 are pro-inflammatory and pro-fibrotic cytokines that increase pulmonary fibrosis due to lung alveolar epithelial cell damage. Tapak liman leaves (Elephantopus scaber L.) have anti-inflammatory and anti-asthmatic properties. This study aimed to investigate the effects of the Elephantopus scaber L. ethanol extract (ESEE) on IL-6 and IL-17 produced by CD4+ and CD8+ in the bleomycin-induced pulmonary fibrosis mice model. Fifty-six male BALB/c mice will be divided into seven groups consisting of healthy mice (N), vehicle mice (V), pulmonary fibrosis (PF), Dexamethasone (DEX) as a drug control, and three doses of ESEE (0.0504, 0.1008, and 0.2016 mg.kg-1 BW). ESEE will be administered orally, followed by intraperitoneal bleomycin injection for 14 days. Mice are then dissected on days 7 and 14, and the spleen will be isolated for analysis of the expression of IL-6 and IL-17. The results showed that ESEE effectively reduced levels of IL-6 and IL-17 cytokines produced by CD4+ and CD8+ T cells, and doses three of ESEE (0.2016 mg.kg-1 BW)  (0.2016 mg.kg-1 BW) showed the most effective reduction activity than the Dexamethasone group. The treatment was proven to reduce the expression of IL-6 and IL-17 in mice with a model of pulmonary fibrosis. Keywords: bleomycin, Elephantopus scaber L., IL-6, IL-17, pulmonary fibrosis.
CaCO3-Reformulated Interlocking Bricks: Physical Characterization and Their Antibacterial and Anti-Inflammatory Potential for Healthy Housing Djati, Muhammad Sasmito; Rachmawati, Turniningtyas Ayu; Anggraini, Retno; Zacoeb, Achfas; Rifa`i, Muhaimin; Minang, Bony Zulkarnaen; Kusuma, Kavana Hafil; Prima, Alex; Christina, Yuyun Ika
Biotropika: Journal of Tropical Biology Vol. 13 No. 1 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.01.01

Abstract

Calcium carbonate (CaCO3) has many benefits for medicine, manufacturing, and nanotechnology due to its antibacterial and anti-inflammatory properties. Interlocking bricks (i-bricks) are widely used in sustainable housing for efficient air circulation and reducing humidity inside the house. If CaCO3 is mixed with interlocking brick, it can inhibit bacterial growth, leading to a healthier indoor environment. Therefore, this study aimed to investigate the physical characterization and antibacterial activity of CaCO3-reformulated i-brick, and its anti-inflammatory potential in mice infected with Salmonella typhimurium. The physical characterization of CaCO3-reformulated i-brick (F1-F10 formulas), including its density, compression, and absorption, was analyzed. The inhibitory activity of CaCO3-reformulated i-brick on S. typhimurium growth was determined using a linear regression equation between culture age and cell density values. BALB/c male mice were randomly divided into four groups (n=4): healthy mice (N), S. typhimurium-infected mice (S), S. typhimurium-infected mice treated with F6 formula (F6) and F7 formula (F7) for 7 days. After 7 days of treatment, lymphocytes were isolated to determine the relative number of TGF-beta, interleukin (IL)-10, and TNF-alpha using flow cytometry analysis. The results showed that formulas 6 and 7 of CaCO3-reformulated i-bricks exhibited optimal physical properties, including density, compression, and water absorption. These formulas also inhibited the growth of S. typhimurium. Furthermore, formulas 6 and 7 possessed anti-inflammatory effects in S. typhimurium-infected mice indicated by the high increase in IL-10 and TGF-beta production and low levels of TNF-alpha. In conclusion, CaCO3 brick formulation can inhibit the growth of S. typhimurium and exert an anti-inflammatory effect. Further investigation is needed to reveal the effect of CaCO3-reformulated i-bricks on another inflammatory marker to further elucidate its anti-inflammatory mechanism.
Antioxidant Properties and Quantification of Phenolic and Flavonoid Compounds in Alpinia purpurata (Viell.) K. Schum Ethanol Extract Djati, Muhammad Sasmito; Azerlyn, Defiona Rensia Naomi; Kusuma, Kavana Hafil; Rosyadah, Nuraini; Kamila, Fairuz Sarah; Annisa, Yuslinda; Christina, Yuyun Ika; Dwijayanti, Dinia Rizqi; Widodo, Nashi
Biotropika: Journal of Tropical Biology Vol. 13 No. 1 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.01.02

Abstract

Phenolic and flavonoid compounds have been found to have positive benefits due to their antioxidant activity. Alpinia purpurata (Viell.) K. Schum, or lengkuas merah, is known to have aromatic rhizomes and is rich in active compounds such as flavonoids and phenolics. This research aimed to investigate the total phenolic and flavonoid content of A. purpurata ethanol extract and its antioxidant activity. A. purpurata was extracted using Microwave-Assisted Extraction (MAE) with 97% ethanol solvent. The ethanol extract of A. purpurata was then analyzed for the total phenolic and flavonoid content. The antioxidant activity and nitric oxide (NO) levels were determined by DPPH and NO scavenging assay, respectively. The results showed that A. purpurata ethanol extract had a high phenolic content (148.76 ± 1.03 mg GAE.g-1extract) but weak DPPH scavenging activity (IC50 of 219.06 ± 8.15 µg.mL-1), indicating that high phenolic content does not necessarily correlate with strong antioxidant properties. In contrast, the extract exhibited strong NO scavenging activity with an IC50 of 22.73 ± 7.57 µg.mL-1. Despite its high phenolic content, the weak DPPH activity indicates that total phenolic concentration alone is not always a reliable indicator of antioxidant strength. Further studies should include additional antioxidant assays to comprehensively evaluate the extract’s antioxidant potential.
Analysis Activity of Elephantopus scaber Leaves Extract Against Quantitative Changes of Lymphocytes Cells in BALB/c Mice After Induction of DMBA and Estrogen Rochmatika, Lailiyavina; Widyarti, Sri; Djati, Muhammad Sasmito
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1127.489 KB) | DOI: 10.21776/ub.jels.2019.009.03.08

Abstract

The carcinogenic compounds such as DMBA known for having the ability to promote the DNA damage that affects the growth regulator genes. Therefore, uncontrolled cell growth will occur and change the immune system. Elephantopus scaber extract is considered as an alternative way of handling breast cancer since it capable to affect the work of the immune system. Hence, this study aims to determine the effect of E. scaber extract on quantitative changes of CD4+, CD8+ T cells and proinflammatory cytokines CD4+IFNγ+ and CD4+TNF-α+ after induction of DMBA and estrogen. In vivo experiments were carried out by using 5-6 weeks old of female mice BALB/c strain. The extract of E. scaber leaf powder was obtained from Material Medica Batu, Malang. The experimental animals were divided into five groups such as negative control group (K-), positive control group (K+), mice treated with E. scaber extract for a week (group 1), mice treated with E. scaber extract for 2 weeks (group 2), and mice treated with E. scaber extract for 3 weeks (group 3). Induction of DMBA and estrogen was conducted by injection of DMBA (7.12 dimetylbenz (α) anthracene) 0.56 mg.kgBW-1 and estradiol hormone 0.0504 mg.kgBW-1. Mice were treated with E. scaber extract with a dose of 50 mg.kgBW-1. Lymphocytes were isolated from spleen and observed the CD4+, CD8+ T cells and proinflammatory cytokines CD4+IFN-γ+ and CD4+TNFα+ T cells through flow cytometry analysis. One-way ANOVA (p <0.05) and SPSS were used to analyze this data. The results showed that the induction of DMBA and estradiol hormone affected mice fur loss. It also decreased the immune system by lowering the number of CD4 and CD8 T cells. However, E. scaber extracts increased the number of pro-inflammatory cytokines TNF-α. Furthermore, the DMBA and estradiol induction also promoted the number of CD4+IFN-γ+ in the first week, but decreased the number of CD4+IFN-γ+ at weeks 2 and 3, then increase the number of CD4+IFN-γ+. So, the treatment of E. scaber extracts demonstrated the ability to restore the homeostasis of the immune system in the first and second weeks.  Keywords: CD4+, CD8+, DMBA, estrogen, E.scaber, IFN-γ+, TNF-α+
Corrigendum: Evaluating SARS-CoV-2 Spike Protein Transfection in HEK-293T Cells for VLP Applications Rohmah, Ilmiana Nurur; Marlita, Marlita; Kusuma, Kavana Hafil; Christina, Yuyun Ika; Dwijayanti, Dinia Rizqi; Mustikaningtyas, Dewi; Widodo, Nashi; Djati, M. Sasmito
The Journal of Experimental Life Science Vol. 15 No. 2 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.02.01

Abstract

The spike protein of SARS-CoV-2 is crucial for initiating infections by binding to host cells and mediating membrane fusion. In this study, HEK-293T cells were transfected with plasmids encoding three structural proteins of SARS-CoV-2, i.e., Spike (S), Membrane (M), and Envelope (E). This transfection enabled the formation of SARS-CoV-2 Virus-Like Particles (VLPs), which allows for safer studies of the virus and its proteins. To serve as a marker for expression, an enhanced green fluorescent protein (EGFP) was fused to the spike protein, resulting in a Spike-EGFP (S-EGFP) fusion protein. The characteristics of SARS-CoV-2 spike protein expression in the transfected HEK-293T cells were then investigated using polymerase chain reaction (PCR) and flow cytometry. The PCR analysis revealed non-specific DNA band smearing, which did not provide conclusive confirmation of spike protein expression. However, flow cytometry analysis demonstrated that approximately 30% of the transfected cells exhibited green fluorescence, indicating the expression of the Spike-EGFP fusion protein. These findings, obtained through flow cytometry, confirmed the successful spike protein expression in transfected HEK-293T cells.  
ULTRASTRUKTUR OOSIT KAMBING PASCA KRIOPRESERVASI DENGAN METODE VITRIFIKASI Wahjuningsih, Sri; Djati, Sasmito
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i2.915

Abstract

Tujuan penelitian adalah mengetahui ultrastruktur oosit pasca vitrifikasi menggunakan transmisi elektron mikroskop. Ovarium dikumpulkan dari rumah potong hewan dan oosit dikoleksi dengan cara aspirasi dari folikel menggunakan jarum ukuran 18 G. Setelah dilakukan klasifikasi kualitas oosit, maka oosit yang berkualitas A dimaturasi secara in vitro. Oosit hasil maturasi dipaparkan ke dalam larutan vitrifikasi yang mengandung 30% EG + 0,5 M sukrosa dengan lama waktu paparan 3 menit, oosit dimasukkan ke dalam ministraw 0,25 cc (French straw), kemudian dipaparkan pada uap nitrogen selama 10 detik dan dimasukkan dalam kontainer nitrogen cair. Setelah 3 minggu ministraw diambil dan dilakukan penghangatan (warming) di udara selama 10 detik, dimasukkan dalam waterbath suhu 35 C selama 30 detik. Isi ministraw dituangkan ke dalam cawan petri dan oosit dibilas dua kali dengan sukrosa 0,5 M. Selanjutnya dilakukan pengamatan ultrastruktur oosit dengan transmisi elektron mikroskop. Hasil penelitian menunjukkan oosit hasil vitrifikasi mempunyai zona pelusida yang abnormal (fraktur), mem bran plasma mengalami lisis, beberapa butir korteks mengalami degenerasi dan perpindahan butir korteks di ruang perivitelin. Kesimpulan dari penelitian ini adalah terdapat perubahan ultrastruktur pada oosit setelah vitrifikasi.
PENGARUH INDUKSI EPIDERMAL GROWTH FACTOR (EGF) TERHADAP PROTEIN Cx43 SELAMA EKSPANSI SEL KUMULUS Umi Baszary, Chomsa Dintasari; Sumitro, Sutiman Bambang; Djati, Mochammad Sasmito; Widjajanto, Edi
Jurnal Kedokteran Hewan Vol 6, No 1 (2012): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i1.350

Abstract

Tujuan penelitian ini untuk mengetahui pengaruh induksi EGF terhadap lokalisasi Cx43 dan gap junction selama ekspansi sel kumulus. Hasilpenelitian ini menunjukkan bahwa ekspansi sel kumulus mempunyai signifikansi dengan meningkatnya konsentrasi EGF dan waktu kultur. Ekspansi sel kumulus diindikasikan dengan perubahan bentuk sel menjadi memanjang dan menyebar. Ekspresi Cx43 meningkat pada kultur jam ke-5 dan semakin meningkat pada kultur jam ke-10 dengan semakin padatnya protein pada badan sel. Pada kultur jam ke-15 terjadi penurunan ekspresi Cx43 pada badan sel. EGF berpengaruh terhadap peningkatan ekspansi sel kumulus tetapi tidak pada ekspresi Cx43 denganbertambahnya konsentrasi dan waktu kultur. Hal ini menunjukkan bahwa komunikasi antar sel kumulus menurun, karena jarak antar sel yang semakin jauh.
Co-Authors Achfas Zacoeb Agatha Maria Dian Kusumawati Ahmad Imron Rozuli Ahmad Rudijanto Ahmad Shobrun Jamil Ahmad Soni Ahmad Soni Ahmed Hasan Abkar Amin Setyo Leksono Aminullah, Lela - Anak Agung Gede Sugianthara Andi Rizki A, Pradana Andi Rizki Adi Pradana Andi Rizki Adi Pradana Andi Rizki Adi Pradana, Andi Rizki Anis Artiyani Annisa, Yuslinda Aris Soewondo Asfi, Nida Asyhari, Firda Nuri Aya shofiyah Azerlyn, Defiona Rensia Naomi Bagyo Yanuwiadi Chomsa Dintasari Umi Baszary Chomsa Dintasari Umi Baszary Dewi Liesnoor Setyowati Dewi Mustikaningtyas Dinia Rizqi Dwijayanti Dliyauddin, Moh Edi Widjajanto Edi Widjajanto Edi Widjajanto Eko Ganis Sukoharsono Eko Puji Astuti El Baroroh, Alif Rosyidah Erin Kurnianingtyas Erly Nur Aisyah Faiza Tawati Fatchiyah Fatchiyah Gato Gato Grahadi, Rahmat Hafil Kusuma, Kavana Handono Kalim Hendra Susanto Henny Johanna Kambey Herminah Febrianty Herminah Febrianty, Herminah Hindun Habibu HUSNUL KHOTIMAH Ida Idewa Agung Willy Pramana Ika Christina, Yuyun Indriati Dwi Rahayu Izzah, Fathiyah Nurul Jamhari Jamhari Jannah, Nur Kamila, Fairuz Sarah Khodijah, Riska Amalia Kliwon Hidayat Kusuma, Kavana Hafil Kuswati Kuswati Lulut Dwi Nurmamulyosari Lulut Dwi Nurmamulyosari M Aris Widodo Mansur Ibrahim Marlita Marlita, Marlita Miasih, Dewi Sekar Minang, Bony Zulkarnaen Mohammad Rasjad Indra Muhaimin Rifa&#039;i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa’i Muhaimin Rifai Muhaimin Rifa’i Muhaimin Rifa’i Muhaimin Rifa’i Muhammad Basyaruddin Muhammad Basyarudin Muhammad Halim Natsir Mutya Farsely Nabilah A Jatiatmaja Nabilah, Sarah Nahdah Nafisah, Wirdatun Nashi Widodo Nelwan, Ester Jeini Nida Asif Noer Hasanah Nur Hidayat Nur Jannah Nurul Faizah NURUL FAIZAH Oktavia Fitri Santika Petrus Sadsoeitoeboen Prima, Alex Putri, Nenis Try Melani Rachmawati , Hidajah Retno Anggraini Retty Ratnawati Rifa'i, Muhaimin Rifa'i, Muhaimin Rifa'i, Muhaimin - Rifa`i, Muhaimin Rifa’i, Muhaimin Rini Dwi Wahyuni Ririn Rochmawati Riska Annisa Rizki Amalia Rizqi Dwijayanti, Dinia Rochmatika, Lailiyavina Roffico Roffico Rohmah, Ilmiana Nurur Rony Irawanto Rooije R.H. Rumende Rosyadah, Nuraini Satuman Satuman septi utami dewi Shofiyah, Aya Siti Aisah SITI AISAH SN Nurul Makiyah soehartojo Hardjopranjoto Soemarno Soemarno Soemarno Soni, Ahmad Sri Andarini Sri Rahayu SRI RAHAYU Sri Rahayu Sri Rahayu Lestari Sri Wahjuningsih Sri Widyarti Sunaryono Sunaryono, Sunaryono Susanti, Winda Karina Susilo Sutiman B. Sumitro Sutiman Bambang Sumitro Swastika Pinca Pinca Swastika Pinca Pinca Syabril Ulum, Akhdiyat Syamsul Arifin Tri Eko Susilorini Turniningtyas Ayu Rachmawati Warsito Warsito Widodo Widodo Widya Ayu Prasdini Widyananda, Muhammad Hermawan Wike andre Septian Yayu Fuadah Yayu Tsamrotul Fuadah, Yayu Tsamrotul Yenny Risjani Yuli Prayitno Yuyun Ika Christina Zulfatim, Heni Sukma