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All Journal HAYATI Journal of Biosciences Health Science Journal of Indonesia Indonesian Journal of Tropical and Infectious Disease Medula JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) The Indonesian Biomedical Journal BIOMA : Jurnal Biologi Makassar Nusantara Medical Science Journal Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Paediatrica Indonesiana Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Majalah Farmasi dan Farmakologi (Trends in Pharmacy and Pharmaceutical Sciences) Indonesian Contemporary Nursing Journal (ICON Journal) Jurnal Ilmu Alam dan Lingkungan Biosaintifika: Journal of Biology & Biology Education Alami Journal (Alauddin Islamic Medical) Journal Nurse and Health: Jurnal Keperawatan JKM (Jurnal Kebidanan Malahayati) Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Jurnal Biomedika Jurnal Riset Kesehatan Poltekkes Depkes Bandung Poltekita : Jurnal Ilmu Kesehatan Medika Alkhairaat : Jurnal Penelitian Kedokteran dan Kesehatan PREPOTIF : Jurnal Kesehatan Masyarakat Jurnal ABDINUS : Jurnal Pengabdian Nusantara Journal of Telenursing (JOTING) Indian Journal of Forensic Medicine & Toxicology Jurnal Midwifery Narra J Sasambo Journal of Pharmacy Jurnal Medika: Media Ilmiah Analis Kesehatan
Muh. Nasrum Massi
Microbiology Department, Faculty Of Medicine, Universitas Hasanuddin
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Journal : Nusantara Medical Science Journal

 1 
DETECTION OF NONTUBERCULOUS MYCOBACTERIA (NTM) IN ISOLATES FROM SUSPECTED TUBERCULOSIS USING PCR IS6110 GENE Andi Nur Asrinawaty; Muh Nasrum Massi; Rizalinda Sjahril
Nusantara Medical Science Journal Volume I No. 1 Januari-Maret 2016
Publisher : Faculty of Medicine, Hasanuddin University.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/nmsj.v0i1.2203

Abstract

ABSTRACT  Introduction: NTM is a group of mycobacterium other than M.tuberculosis complex and M.Leprae, which found in the environment and can be pathogenic. Objectives: This study aim was to detect NTM isolates using PCR on target genes IS 6110. Methods: This study was conducted in Laboratory of Microbiology, Faculty of Medicine, Hasanuddin University and Laboratorium microbiology in Hasanuddin University Hospital. 14 sample isolates of NTM from sputum of suspected tuberculosis patients. The method using PCR IS6110 gene. Results: The results showed that of 14 samples, 8 positive samples with M. tuberculosis and 6 samples negative. Conclusions: IS6110 PCR can be used to detect NTM isolates from sputum of suspected tuberculosis patients. Keywords : NTM, PCR, IS6110, M.tuberculosis
THE ASSOCIATION OF POLYMORPHISM PROMOTER VEGF PROMOTER GENE POLYMORPHISM AND VEGF SERUM LEVEL WITH THE TYPE OF AGE-RELATED MACULAR DEGENERATION Andi M Ichsan; Habibah S Muhiddin; Nasrum Massi; Budu Budu
Nusantara Medical Science Journal Volume 1 No. 2 April - Juni 2016
Publisher : Faculty of Medicine, Hasanuddin University.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/nmsj.v1i2.2211

Abstract

ABSTRACT Introduction: One of the potential genetic links to Age-related macular degeneration (AMD) is vascular endothelial growth factor (VEGF). The aim of this study was to investigate the association of polymorphism promoter VEGF gene and VEGF serum level with the type of AMD. Methods: Genomic DNA was extracted from peripheral blood of 66 samples, consist of wet and dry AMD, and age-matched controls. DNA was subjected to polymerase chain reaction amplification at the position -460 coupled with the restriction fragment length polymorphism. VEGF serum level was measured by Enzyme-linked Immunosorbent Assay. Results: The polymorphism promoter VEGF gene demonstrated significant association with the type of AMD (p=0.004, OR=3.1 95% CI 1.1-8.6) and the risk for increasing VEGF serum level (p=0.011, OR=3.2 95% CI 1.18.9). Despite there was no significant association between dry and wet AMD with VEGF serum level (p=0.081), but this study revealed significant increased of VEGF serum level in AMD patients, compared to those in control’s serum (p<0.001). Finally, there was coassociation between polymorphism and VEGF level with the type of AMD (p<0.001). Conclusion: This study reveals the significant association between promoter VEGF gene polymorphism and VEGF serum level with the type of AMD of Indonesian patients.  Keywords: AMD, VEGF gene polymorphism; VEGF serum level.
THE IMPACT OF THE FREQUENCY OF 7,12- DIMETHYLBENZ [Á] ANTHRACENE (DMBA) ADMINISTRATION ON THE FORMATION OF DYSPLASIA AND SQUAMOUS CELL CARCINOMA ON THE SKIN OF ALBINO MICE Diana Muchsin; Khairuddin Djawad; Alwi A Mappiasse; Idham Jaya Ganda; Nasrum Massi; Gemini Alam
Nusantara Medical Science Journal Volume 1 No. 4 Oktober - Desember 2016
Publisher : Faculty of Medicine, Hasanuddin University.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/nmsj.v1i4.2248

Abstract

ABSTRACT Introduction: Dosage and frequency of 7,12-dimethylbenz[á] anthracene (DMBA)  com- pound exposure suspected to affect the rapid growth of squamous cell carcinoma (SCC) in albino mice. Objective: find out the impact of DMBA exposure to dysplasia and SCC formation on albino mice. Methods: Twenty-five albino mice were divided into five groups, namely: Group 1 is the control (acetone), group 2 is 5 albino mice given four times DMBA100 µg exposure for eight weeks, group 3 is 5 albino mice were given eight times DMBA100 µg exposure for eight weeks, group 4 is 5 albino mice were given twelve times DMBA100 µg exposure for eight weeks, and group 5 is 5 albino mice were given sixteen times DMBA 100 µg exposure for eight weeks. Histopathological examination were conducted to assess histopathological feature including inflammation, dysplasia, and SCC. Results: There is a significant effect of the frequency of DMBA exposure to the clinical figure and against histopathological feature of the skin albino mice.Conclusions:  Dosage and fre- quency of DMBA exposure affect the occurence of dysplasia and SCC, which are in- creasingly being DMBA exposure will accelerate the growth of SCC. The frequency of the most widely pose a SCC in DMBA 100 µg sixteen times exposure group, twice a week for eight weeks. Keywords: DMBA, dysplasia, squamous cell carcinoma
DETECTION OF SHIGELLA SP ON STOOL CHILDREN WITH DIARRHEA USING CULTURE METHODE AND PCR Rusdianto Rusdianto; Muh Nasrum Massi; Ahyar Ahmad
Nusantara Medical Science Journal Volume 1 No. 4 Oktober - Desember 2016
Publisher : Faculty of Medicine, Hasanuddin University.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/nmsj.v1i4.2250

Abstract

ABSTRACT Introduction: Diarrhea is one health problem in the world with mortality and morbidity rates are high, both in developed and developing countries. This research aims to detect Shigella sp in the stool of children with diarrhea using culture method and PCR. Methods: This research is a descriptive with cross-sectional design. A total of 50 samples were obtained from four health centers in Makassar city, i.e Barabaraya, Pampang, Antang Perumnas and Tamangapa from May - July 2016 with rectal swab method. For culture test, the samples were inoculated on medium SSA. While with PCR test, primer icsA (526 bp) was used. Results: The results indicate that, from 50 samples tested by using culture method, there is no sample (0%) posi- tive detected Shigella sp. While the PCR method there 6 samples (24%) positive detected Shigella sp. Conclusion: The sensitivity of PCR in the detection of Shigella sp from the level of dilution 100 - 10-3. Al together, it can be inferred that PCR method is more accurate method detect Shigellasp than other culture methode. Keywords: Diarrhea, Shigellasp, Culture, PCR
DETECTION OF ESCHERICHIA COLI IN CHILDREN STOOL WITH DIARRHEA PATIENTS USING CULTURE AND POLYMERASE CHAIN REACTION METHOD Lokita Purnamasari; Muh Nasrum Massi; Ahyar Ahmad
Nusantara Medical Science Journal Volume 2 No. 1 Januari - Juni 2017
Publisher : Faculty of Medicine, Hasanuddin University.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/nmsj.v2i1.2256

Abstract

ABSTRACT Introduction : Diarrheal diseases are  a serious issue on health in developing countries and the liquid cause’s morbidity and mortality in children that cause the diarrhea pathogenic bacte- ria including Escherichia coli.  This research aims to detect E. coli in stool diarrhea patient of the  child with culture method and  Polymerase Chain Reaction. Methods : This research is descriptive research design with cross-sectional approach. The sampling was collected from some public health centers namely Pampang, Barabarayya, Antang  Perumnas, Tamangapa public health centers. Specimen testing process was conducted at Microbiology laboratory of the Teaching Hospital University of Hasanuddin to identify E. coli bacterium with the culture and  to detect eae gene and bfp with PCR technique. Results : The results indicate that from50 samples, there  are  15 samples (30%) of samples positively detection E. coli using culture method and  PCR  test using eae and  bfp primer found 20 (40%) and  1 sample (2%) positive enteropathogen E. coli. Conclusion : PCR  methods indicated the  result of E. coli bacteria results faster and more accurate that other  culture methods. Keywords : Diarrhea, Escherichia coli, Polymerase Chain Reaction
Co-Authors Adyatma Muhammad Nur Agus Sangka Pratama Ahmad, Mardiana Ahyar Ahmad Alwi A Mappiasse Amiruddin Laompo Anas, Lukman Andi Alfian Zainuddin Andi Ariyandi Andi Asadul Islam Andi M Ichsan Andi Nilawati Usman Andi Nur Asrinawaty Andi Rofian Sultan Andi Zulkifli AS Anita Anita Ari Yuniastuti Arif, Sitti M. Arsyad, Andi Fatimah Asaad Maidin Asty Amalia Nurhadi Azhar, Muh. Aprizal Baedah Madjid Bahrul Fikri Budu - Budu Budu Bukhari, Agussalim Chalid, Maisuri Tadjuddin Conny Tanjung Dasril Daud DAVID HANDOJO MULJONO Dewi Mutisari Dian Amelia Abdi Diana Muchsin Dwi Kesuma Sari Eddyman W Ferial Faisal Attamimi Faradillah Suhar Febriani, Dwi Bahagia Ferra Indrawati Fikri, Bahrul Firdaus Hamid Firdaus Kasim Furqan Zakiyabarsi Gemini Alam Gemini Alam Habibah S Muhiddin Hendra Wibawa Herlina Rante Hidayah, Najdah Hursepuny, Valentine Ibrahim Labeda Idham Jaya Ganda Ilhamjaya Patellongi Ilhamjaya Pattelongi Irawan Yusuf Irawati Djaharuddin Irma Andriani Irma Andrianys Kasmiati Kasmiati Khairuddin Djawad Khairuddin Djawad Kusrini S. Kadar Latief, Nikmatia Linda Runtuwene Lisa Tenriesa Lokita Purnamasari Luthfi, Muhammad M Natsir Djide Marcellus Simadibrata Mardiana Ahamad Mardiana Ahmad Mardiana Ahmad Meutiah Ilhamjaya Mochammad Hatta Mochammad Hatta Mochammad Hatta Muflihanah Muhammad Akmal A. Sukara Mustika Hutabarat Sari Mustika Sari Nadirah Rasyid Ridha, Nadirah Rasyid Nadyah Nasir Djide Nilawati Usman Nilawati, Andi Norma Tiku Kambuno Nur Fajri Nurfadillah S Nurmadilla, Nesyana Nurpajri, Nurpajri Palantei, Elyas Palulun, Prajayanti Pattelongi, Ilhamjaya Prihantono Prihantono Putri, Sri Hardiyanti Raden Tina Dewi Judistiani Rahmawaty Rahimi Rahmi Rahmi Rajia Rasita, Yoeke Dewi Ratna Ridha Wahyuni, Ridha Rina Masadah Rinda Nariswati Ritha Pratiwi Riza Linda Rizalinda Sjahril Rizalinda Sjahril Rosana Agus Rosdiana Natzir Rusdianto Rusdianto Safruddin Amin Saidah Syamsuddin Samara, Yulianti Sari Handayani Sartini Sartini Sharvianty Arifuddin Sharvianty Arifuddin Shelly Salmah Sjafaraenan Sjafaraenan, Sjafaraenan Solly Aryza Sri Vitayani Suanti Sudirman Katu Sudirman Katu, Sudirman Suhuyanly, Nella Supri Suryani As'ad Syahril, Rizalinda Syakib Bakri Tjahjadi Robert Tedjasaputra Turyadi Turyadi, Turyadi UPIK ANDERIANI MISKAD, UPIK ANDERIANI Veni Hadju Wardihan Sinrang Yenti Purnamasari Yoeke Dewi Rasita Zakiani Sakka