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All Journal International Journal of Public Health Science (IJPHS) HAYATI Journal of Biosciences Bioscience Jurnal Teknologi Pangan dan Gizi Pharmaciana: Jurnal Kefarmasian Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Indonesian Journal of Biotechnology Journal of Agromedicine and Medical Sciences Biota Bioeksperimen: Jurnal Penelitian Biologi JURNAL PENGABDIAN KEPADA MASYARAKAT Journal of Tropical Biodiversity and Biotechnology Science and Technology Indonesia Medical Laboratory Technology Journal Jurnal Biodjati THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST JURNAL GALUNG TROPIKA Jurnal Biologi Tropis BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains JURNAL KESEHATAN PERINTIS Jurnal Ilmu Kefarmasian Indonesia BioEksakta : Jurnal Ilmiah Biologi Unsoed Jurnal Pengabdian kepada Masyarakat Nusantara Nusantara Science and Technology Proceedings Jurnal Insan Farmasi Indonesia Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks Journal of Biotechnology and Natural Science Jurnal Katalisator Organisms: Journal of Biosciences Medical Sains : Jurnal Ilmiah Kefarmasian Buletin Dharmas Andalas
Maliza, Rita
Biology Department, Faculty Of Mathematics And Natural Sciences, Andalas University, Padang 25175, Indonesia
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Unravelling the Potential of Wild Endemic Sumatran Turmeric (Curcuma sumatrana Miq.) as a Candidate of Herbal Medicine for Neurodegenerative Diseases Putra Santoso; Syafruddin Ilyas; Rita Maliza; Indah Fajarwati; Rauza Sukma Rita; Aldi Tamara Rahman; Jihan Aprilia Nawawi; Ivan Petrovsky; Muhammad Samudra Ilham; Ananda Fitriana
Science and Technology Indonesia Vol. 11 No. 3 (2026): July
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2026.11.3.1212-1240

Abstract

Neurodegenerative diseases pose significant health challenges, while synthetic drugs are often associated with detrimental side effects. Hence, the search for herbal medicines is warranted. Curcuma sumatrana is traditionally used by Minangkabau people as a herbal medicine, but it remains scientifically underexplored. This study aims to evaluate its neuroprotective potential through phytochemical profiling, computational analyses, and biological assays. LC-MS/MS-QTOF profiling identified 13 compounds in the rhizome extract, predominantly terpenoids. In silico analyses predicted antioxidant, anti-inflammatory, and antineurodegenerative activities, while drug-likeness assessment suggested favorable bioavailability for all identified compounds. Furthermore, molecular docking targeting neurodegeneration-associated proteins revealed strong interactions of compounds namely brefeldin A, nigakilactone K, genistein, and 8-epi-loganic acid-6’-O-β-D-glucoside with MAPK1, TNF-α, BACE1, and CASP3, respectively. In addition, the extract exhibited notable antioxidant activity in the DPPH assay and ameliorated monosodium glutamate-induced cognitive impairment, hippocampal neurodegeneration, and elevated brain malondialdehyde levels in mice. Hence, C. sumatrana is a promising herbal medicine for neurodegenerative diseases, plausibly acting through the modulation of neurodegeneration-related pathways and antioxidant mechanisms. These findings provide a foundation for future drug discovery by identifying bioactive compounds with potential applications in the management of neurodegenerative disorders.
Codon Optimized MMLV Reverse Transcriptase Expression and Functional Evaluation in Escherichia coli Elsa Alfiyanti; Andani Eka Putra; Rita Maliza; Alponsin Alponsin
Jurnal Biologi Tropis Vol. 26 No. 3 (2026): Juli - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i3.12798

Abstract

Dependence on imported reverse transcriptase (RT) enzymes remains a challenge for the development of molecular diagnostic reagents, particularly in developing countries. This study aimed to design, express, purify, and functionally evaluate a recombinant Moloney Murine Leukemia Virus reverse transcriptase (MMLV-RT) as a candidate locally produced molecular biology reagent. A codon-optimized synthetic MMLV-RT gene was cloned into the pET-21a(+) expression vector and transformed into Escherichia coli BL21 (DE3). Recombinant protein expression was induced using IPTG, followed by purification using Ni²⁺-NTA affinity chromatography and characterization using SDS-PAGE. Functional activity of the recombinant enzyme was evaluated through reverse transcription of RNA templates followed by conventional PCR amplification. The optimized MMLV-RT gene was successfully expressed in E. coli BL21 (DE3), producing a recombinant protein band at approximately 55.4 kDa. The highest protein yield was obtained after overnight induction, reaching 3.385 mg/mL. Purification resulted in a dominant protein band corresponding to the expected molecular weight of MMLV-RT. Functional evaluation demonstrated that recombinant MMLV-RT successfully generated specific PCR amplicons with performance comparable to commercial reverse transcriptase without detectable non-specific amplification. These findings indicate that recombinant MMLV-RT produced in E. coli BL21 (DE3) retains functional reverse transcriptase activity and has potential for further development as an alternative locally produced reagent for molecular diagnostic applications.  
Co-Authors Adam Raihan Priambada Adi Wira Septama Adilla, Ardis Trianita Adnan, Adnan Afdhal Raihan Afdhal Raihan Afif , Muhamad Aini, Wardahtul Aini, Wardatul Aldi Tamara Rahman Aldi Tamara Rahman Alfiatul Laila Alimuddin Tofrizal Allimuddin Tofrizal Alponsin Alponsin Amelia Roza, Amelia Ananda Fitriana Andani Eka Putra Angga Illahi Kurnia Illahi Angga Kurnia Illahi Anggun Sophia Anisa Nova Puspitaningrum Anthoni Agustien Aprilia Cassa Nova Aprilia, Kharisma Annisa Aris Setiawan Arthauly Yopita Sinurat Asful, Ferdhinal Ashrifurrahman Ashrifurrahman Ashrifurrahman, Ashrifurrahman Asih, Enda Tarni Aszareta, Muhammad Andoni Athika Darumas Putri Aulia Rahmi Aulia, Annisa Ayu Rosemeilia Dewi Aziz, M. Abdul Azizah Amatu Zikrah Azizah Amatu zikrah Azzahra, Layla Bayu Tri Murti Bramadi Arya Cahyani, Dini Chairunnisa Azzahra Azzahra H Cheung, Rocky Dahelmi Dahelmi Diana Fadhilah Diani Fatmawati Dyah Aryani Perwitasari Efa Refiani Efrizal Efrizal Eli Ratni Elisa, Tasya Putri Pratama Elni Fatimah Elsa Alfiyanti Era Refiani Esa Savitri Eti Yerizel Fadhilah, Diana Fadillah Fadillah Fadillah Fadillah Fajri Hidayat Febriofca Galih Yatalaththov Firmansyah, Tio Galih Yatalaththov, Febriofca Gina Noor Djalilah Haafizah Dania Haafizah Dania Haafizah Dania Hamdani, Afif Hamdi Ibrahim, Muhammad Haris Setiawan Harry Nugroho Eko Surniyantoro Hendra Kusuma Henny Herwina Horiza, Hevi Hulrahma, Mifta Husna Fitri Husna Fitri Husna Fitri Husna Fitri Hylda Ibrahim, Muhammad Hamdi Idris, Muhamma Ika Wahyuni Ikrimah Nisa Utami Ilham Samudra, Muhammad Ilham, Kurniadi Illahi, Angga Illahi Kurnia Imaniar Noor Faridah Imaniar Noor Faridah Imaniar Noor Faridah Indah Fajarwati Inggita Utami Irwan , Reziq Marchellino Ivan Petrovsky Ivan Petrovsky Jabang Nurdin Jannati Aulah Janra, Muhammad Nazri Jasmi Jefrial Santoso Jihan Aprilia Nawawi Kania Agustina Kiky Martha Ariesaka Kurniadi Ilham Lalu Muhammad Irham Lestari, Mardiana Puji Liana Fairuzannah Mahdiana Listiatie Budi Utami Lutfi Sukma Pratiwi M.Nazri Janra Made Ary Sarasmita Mahardika, Riris Srikandi Mansyurdin Marchellino Irwan, Reziq Melinda Widianingrum Melinda Widianingrum Melinda Widyaningrum Melodia Rezadhini Miftahul Ilmi Mifthahul Jannah Mildawati Mildawati Moh. Mirza Nuryady Mohammad Abrar Mohsin Rafique Muhammad Afif Muhammad Hamdi Ibrahim Muhammad Idris Muhammad Idris Muhammad Idris Muhammad Idris Muhammad Ilham Hisbullah Muhammad Nazri Janra Muhammad Samudra Ilham Muhammad Syukri Fadil Nabila, Sylvia Nabilah Adzra Fahlevi Nabilah Rahmachila Azura Nadjmir, Mikail Nanik Sulistyani Nastiti Rahajeng Nazri Janra, Muhammad Nelvi, Dennisa Arra Ni Made Ary Sarasmita Nofrita Nofrita Nofrita Nofrita Nopiyanti, Tita Nur Aida Nur Rahmah Hidayati Nurul Annisa Nurwijayanti Oktira Roka Aji Petrovsky, Ivan Pradika, Jaka Pratama Elisa, Tasya Putri Pratama, Kharisma Pratama, Raihan Anugrah Puspitaningrum, Anisa Nova Puti Khairunnajwa Amar Putra Santoso Putri Lestari Putri Pratama Elisa, Tasya Putri, Fadilatul Rahmi Putri, Syntia Mai Raihan, Afdhal Ramadanti, Dhea Rarastoeti Pratiwi Rauza Sukma Rita Resti Rahayu Rezadhini, Melodia Reziq Marchellino Irwan Reziq Marchellino Irwan Ridho, Muhammad Syifa'ur Rifai, Fauziah Novita Putri Rihadatul Aisyah Rilwan Efendi Rismayani, Dessy Rizma Nurul Akhlas Robby Jannatan Rocky Cheung Rocky Cheung Saraswati, Utami Sinurat, Arthauly Yopita Siti Rokhimah Sumaryati Syukur Sutri Wulansari Safril Syabila, Hutri Dinda Syafruddin Ilyas Syaidah, Rahimi Syaiful Adam Maulana Syaifullah Syaifullah Syakira Tiara Rezvi Syamsuardi Syamsuardi Tasya Putri Pratama Elisa Tasya Putri Pratama Elisa Tio Firmansyah Tofrizal Tofrizal , Allimuddin Tofrizal, Alimuddin Tur Rahardjo Utami Saraswati Wardatul Aini Wilson Novarino Wirawan Adikusuma Yanti Lusiyanti Yanti, Windi Nofri Yashiro, Takashi Yeni Alfiana Ratnasari Yudha Rizky Nuari Yustia Tuti Zaini Zaini Zikrah, Azizah Amatu Zozy Aneloi Noli Zozy Aneloi Noni Zuhir, Elvina Zulfikri, Elzam Naufal