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PERBANDINGAN UJI DIAGNOSTIK GENEXPERT MTB/RIF DENGAN KULTUR SENSITIVITAS ANTIBIOTIK DALAM MENDETEKSI RESISTENSI RIFAMPICIN Mycobacterium tuberculosis PADA PASIEN TB PARU DI RSUP H. ADAM MALIK MEDAN Nurbaity Situmorang; Perry Boy Chandra Siahaan; Suharsih; Dewi Novina Sukapiring
Jurnal Biogenerasi Vol. 7 No. 2 (2022): Jurnal Biogenerasi Vol 7 Nomor 2 tahun 2022
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v7i2.1912

Abstract

WHO recommends GeneXpert Mycobacterium tuberculosis; Rifampicin as an initial examination for the diagnose of drug-resistant tuberculosis and drug-sensitive tuberculosis in pulmonary TB patients. The GeneXpert MTB/RIF examination is a molecular examination method with Nucleic Acid Amplification Technology (NAAT) which can diagnose TB and resistance to Rifampicin within 2 hours. The aims of this study is to determine the comparison of GeneXpert MTB/Rif Diagnostic Test with Drug Sensitivity Culture in Detecting Rifampicin M. tuberculosis resistance in TB patients at H. Adam Malik Hospital, Medan. The sample in this study was the entire population of suspected pulmonary TB at H. Adam Malik Hospital Medan who had MTB examination results. Post; rif. res. The sample size was using total sampling, namely the sampling technique where the number of samples was the same as the population, namely 128 patients in 2020. The results of the Rifampicin TCM Antibiotic Test were found that 128 people had Rifampicin Resistant results. Based on the percentage of the results of the MGIT Antibiotic Sensitivity Test, 125 people were found to have Rifampicin Resistant results and 3 people had Rifampicin Sensitive results. Based on the results, it can be seen that the sensitivity value of the TCM tool to DST MGIT culture is 97.7%. The Conclusions of these Research is the ability of the GeneXpert MTB/Rif method to detect rifampin resistance can be used as a screening tool to diagnose rifampin-resistant TB.
ANALISIS PROKSIMAT KERUPUK DAUN JERUJU DARI TANAMAN JERUJU (Acanthus ilicifolius) Lily Novianty; Siti Nurlani Harahap; Dewi Novina Sukapiring
Jurnal Biogenerasi Vol. 8 No. 1 (2023): Volume 8 Nomor 1 Tahun 2023
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v8i1.2286

Abstract

Proximate analysis is an analysis performed to determine the nutritional value contained in a food or processed food. This study aims to determine the water content, protein content, fat content, ash content and carbohydrate content of Jeruju leaf crackers. The method used in this research was an experimental method in the laboratory by analyzing the water content, protein content, fat content, ash content and protein content of Jeruju leaf crackers. From the research conducted, the water content results for the S1 treatment were 3.17 + 0.12; S2 is 6.58 + 0.01 and S3 is 6.51 + 0.53. The resulting protein content for the S1 treatment was 28.65 + 0.52; S2 of 31.78 + 0.53; S3 is 23.97 + 1.05. The resulting fat content for the S1 treatment was 24.49 + 0.06; S2 is 19.74 + 0.01 and S3 is 21.64 + 0.06. The resulting ash content for the S1 treatment was 2.12 + 0.01; S2 is 1.4 + 0.01 and S3 is 1.39 + 0.02. The resulting carbohydrate content for the S1 treatment was 41.55 + 0.5; S2 is 40.46 + 0.5 and S3 is 46.47 + 1. Analysis of water content, protein content and ash content in Jeruju leaf crackers decreased in value while for fat content and carbohydrate content increased the levels if the concentration of Jeruju leaf extract was added to the sample more and more
Peningkatan Kreativitas Siswa Melalui Pemanfaatan Bunga Telang Menjadi Sirup di SMP NU Medan Tuti Wardani Siregar; Dini Novita Sari; Chandra Sigalingging; Dewi Novina Sukapiring; Lily Novianty; Juhardi Sembiring; Nelfita Rizka S. Depari; Khairuna Utami; Emma Fadhilah; Mardiah Mardiah; Saiful Amir; Furqan Khalidy
Jurnal Abdi Masyarakat Indonesia Vol 4 No 3 (2024): JAMSI - Mei 2024
Publisher : CV Firmos

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54082/jamsi.1130

Abstract

Bunga telang atau butterfly pea, memiliki nama latin Clitoria ternatea L. Disebut butterfly pea karena bentuknya menyerupai kupu-kupu. Tanaman yang hidupnya merambat ini dipercaya memiliki segudang manfaat untuk kesehatan. Bunga ini, dapat digunakan sebagai tanaman obat karena adanya senyawa kimia yang terkandung di dalamnya seperti: saponin, Flavonoid, alkaloid, Ca-oksaat dan sulfur. Bunga ini juga dimanfaatkan untuk mengobati mata, sedangkan rebusan akarnya digunakan sebagai obat untuk menghilangkan dahak pada bronkitis kronis, menurunkan deman, serta iritasi kandungan kemih dan saluran air seni. Melihat manfaat bunga telang yang bagus untuk Kesehatan perlunya dimanfaatkan untuk memenuhi kebutuhan sehari-hari. Proses pembuatan sirupcukup mudah, yakni dengan cara mengeringkan Bunga Telang dibawah sinar matahari, kemudian diseduh dengan air panas sehingga menghasilkan biang bunga telang, selanjutnya kita menyediakan gula pasir yang sudah dicairkan, selanjutnya kita menyatukan biang bunga telang dengan gula cair sehingga menjadi sirup bunga Telang. Tujuan kegiatan pengabdian ini dapat dicapai dengan baik berdasarkan ketertarikan siswa/siswi kelas VII SMP NU Medan terhadap pembuatan sirup dari bunga Telang.
Proximate Analysis of Tempeh Nuggets with the Addition of Oyster Mushroom (Pleurotus ostreatus) Siti Nurlani Harahap; Lily Novianty; Dewi Novina Sukapiring
EduMatSains : Jurnal Pendidikan, Matematika dan Sains Vol 9 No 1 (2024): July
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kristen Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/edumatsains.v9i1.5927

Abstract

Basic human needs include nutritional needs. There are an increasing number of new and high-quality foods that perform optimally for human activities. Currently consumers prioritize food that is practical and easy to consume, one of which is nuggets. The popular nuggets is chicken nuggets which have a high fat content and cholesterol which is harmful for health. Currently there are developments of high-fiber nugget innovations through the optimization of tempeh and oyster mushrooms This study aims to analyze the proximate to determine the water content, protein content, carbohydrate content, fat content, and ash content in tempeh nuggets with the addition of oyster mushrooms. The method in this study uses an experimental method carried out in the laboratory by analyzing the water content, carbohydrate content, fat content, ash content, and protein content of the nuggets. Based on the research that has been conducted, the average results of the highest water content of tempeh nuggets combined with oyster mushrooms is in the S3 treatment (51.75%), protein content in the S3 treatment (13.747%), fat content in the S2 treatment (13.69%), ash content in the S1 treatment (6.057%) and carbohydrate content in the S1 treatment (27.113%)
UJI DAYA TERIMA NUGGET TEMPE DENGAN PENAMBAHAN JAMUR TIRAM (Pleurotus ostreatus) SEBAGAI MAKANAN ALTERNATIF PENGGANTI DAGING Nurlani Harahap, Siti; Novianty, Lily; Novina Sukapiring, Dewi
Jurnal Biogenerasi Vol. 8 No. 2 (2023): Volume 8 Nomor 2 Artikel Publish Agustus 2023
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v8i2.2847

Abstract

The nuggets sold in the market are usually made from chicken or beef. However, processed nugget products made from animal protein, namely using chicken and beef, have a high fat content and low fiber, therefore it is necessary to innovate by diversifying animal-based foods with plant-based foods in processed nuggets. This study aims to determine the acceptability of tempe nuggets with the addition of oyster mushrooms as an alternative food to replace meat. The method used in this study was a randomized block design (RBD) which consisted of three treatment levels: P0 = 100% tempeh; P1 = 75% tempe : 25% oyster mushrooms; P2 = 50% tempe : 50% oyster mushrooms; P3 = 25% tempe : 75% oyster mushrooms. Tempe nuggets power test with the addition of oyster mushrooms was carried out using an organoleptic test consisting of 20 panelists with a numerical scale of 5 = very like; 4 = likes; 3 = somewhat like (ordinary); 2=dislike and 1=dislike very much. The P2 treatment, namely 50% tempeh and 50% oyster mushrooms, had the panelist's average preference for taste, aroma, texture and friability of tempeh nuget with the highest value. This could be because the composition might be very good when compared to other compositions.
UJI SENSITIVITAS ANTIBIOTIKA TERHADAP BAKTERI PENYEBAB INFEKSI PADA LUKA PASCA OPERASI Mirza, Mirzan Hasibuan; Suharsih; Situmorang, Nurbaity; Novina Sukapiring, Dewi; Kusumawati, R Lia
Jurnal Biogenerasi Vol. 9 No. 1 (2024): Terbit volume 9 nomor 1 tahun 2024
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v9i1.3331

Abstract

Nosocomial infection is a systemic or local condition caused by a reaction to an infectious agent such as bacteria that occurs more than 48 hours after the patient is admitted to the hospital. One of the nosocomial infections is post-surgery wound infection which is a common problem that causes morbidity and mortality in hospitals. The challenge for doctors who treat infected patients is the accuracy of antibiotic therapy, which slows down the healing process of infection in postoperative wounds. This study aims to determine the sensitivity of antibiotics to bacteria that cause infections in post-surgery patient wounds which can be recommended as definitive therapy. This study is a retrospective cohort conducted descriptively during October 2020 with a total of 75 samples. The results of the study showed that antibiotic sensitivity to gram-negative bacteria was amikacin (91%), meropenem (81%), gentamycin (56%), ciprofloxacin (42%), cefepime (39%), cefoperazone sulbactam (39%), cotrimoxazole (28%), ceftazidime 25%, aztreonam (18%), ceftriaxone (14%), ampicillin-sulbactam (11%) and ampicillin (2%). Meanwhile, sensitivity to gram-positive bacteria is vancomycin (83%), linezolid (61%), gentamycin (61%), clindamycin (39%), erythomycin (39%), levofloxacin (33%), ciprofloxacin (28%), cotrimoxazole (22%) and ampicillin, cefotaxime, ceftazidime, ceftriaxone (17%), respectively. Antibiotics with a sensitivity of >80% that can be recommended as definitive therapy for post-operative wound infections are amikacin (91%) and meropenem (81%) for gram-negative bacteria and vancomycin (83%) for gram-positive bacteria.
Peningkatan Literasi Siswa SD Negeri 106204 Tanjung Raja Melalui Pojok Baca Hendra; Nuri Ramadhan; Leni Malinda; Emy Hariati; Sisilia Florina Yanti; Nurbaity Situmorang; Dewi Novina Sukapiring; Hendri Irawan; Muhammad Khaibar Putra Adhitia; Nurul Ajima Ritonga; Astria Ayu Ramadianti; Syarifah Ainun Harahap
Jurnal Pengabdian Masyarakat Vol. 2 No. 1 (2023): Juni 2023
Publisher : Unity Academy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70340/japamas.v2i1.57

Abstract

With children's interest in reading still low, the government launched the School Literacy Movement (GLS). This program is expected to foster interest in reading in students as well as to build a literacy climate in schools. However, not all schools have been able to implement GLS in accordance with the guidelines provided. The problems include inadequate facilities and infrastructure, limited reading materials, limited reading time. The purpose of this community service is to develop reading literacy in students at UPT Elementary School in launching GLS through books and reading trees provided by the PKM team to each partner school. Activities in assisting the making of this class reading corner are carried out by the team and class teachers at the State Elementary School, starting from preparing the class that will later be made a class reading corner, the materials needed in making the reading corner, the books to be donated, and the teacher's role in mentoring later. The team will also donate basic education level books and folklore books to fulfill the bookshelves that will be made in the reading corner later. The end result of this activity is expected that the reading corner that has been made will be emulated by teachers in other classes and the donated books will be useful for teachers and students and schools.
SOSIALISASI MENCUCI TANGAN DENGAN BENAR DAN PELATIHAN PEMBUATAN PAPER SOAP DI SMP NEGERI 1 SORKAM, KECAMATAN SORKAM KABUPATEN TAPANULI TENGAH Emy Hariati; Hendra; Nuri Ramadhan; Leni Malinda; Sisilia Florina Yanti; Nurbaity Situmorang; Dewi Novina Sukapiring; Mastari Ramadhani; Juhardi Sembiring; Rizky Ananda; Fatimah Pohan; Suharsih; Syarifah Ainun Harahap; Rara Astili Siregar
Jurnal Pengabdian Masyarakat Vol. 3 No. 1 (2024): Juni 2024
Publisher : Unity Academy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70340/japamas.v3i1.117

Abstract

Regular handwashing is the first 5M health protocol and is effective in preventing the transmission of viruses and diseases. However, mobility and activities cause many to neglect to always have soap on hand when travelling. For this reason, a simple innovation for the use of soap is needed, namely by using paper soap. Paper soap is much more practical in its use and easy to carry anywhere. Based on the survey of the service team, several problems were found, such as students who were often absent from school due to illness, students' low understanding of hand washing with soap, and students' lack of knowledge in making paper soap. The purpose of this community service is to improve students' knowledge and skills in applying the six steps of handwashing with soap and to improve students' skills in making paper soap with natural ingredients. This community service was carried out by providing counselling on the importance of hand washing, assistance in making paper soap from natural ingredients to students. The results obtained from this service are an increase in knowledge and creativity of students who initially did not know how to make paper soap to know, and increased student understanding of the steps of good and correct hand washing.
Inventory of Pathogenic Fungi by Red Chili Seeds (Capsicum annuum L.) from Deli Serdang Regency, North Sumatera Sukapiring, Dewi N; Novianty, Lily; Harahap, Siti Nurlani
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i3.4894

Abstract

Red chilies known as Capsicum annuum L., hold significant importance as horticulture crops in Indonesia. The production of red chilies begins with the planting of seeds, making seed health a crucial factor in the process. Seeds contaminated with pathogens can serve as a reservoir for the transmission of diseases in the field, leading to the demise of seedlings and hindering their growth into fully mature plants, ultimately resulting in crop failure. This study used the blotter test technique to ascertain the morphology of a seed-borne pathogenic fungus. The research findings indicated that the blotter test technique detected four isolates of seed-borne pathogenic fungus, specifically TMIA with an infection rate of 0.5%, TMIB with an infection rate of 0.5%, TMIC with an infection rate of 2.5%, and TMIE with an infection rate of 3%. A single isolate of a seed-borne pathogenic fungus, named SRIA, was obtained from Sidodadi Ramunia village. The infection rate of this fungus was determined to be 0.5%. The chili seeds recovered were found to carry pathogenic fungal isolates belonging to Aspergillus sp., Penicillium sp., Verticillium sp., and Curvularia sp. based on morphological identification
Molecular Identification of Fungi carried by Chili Seeds (Capsicum annuum L.) from Tanjung Morawa B Village, North Sumatra Sukapiring, Dewi Novina; Novianty, Lily; Harahap, Siti Nurlani
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 10, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2024
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i2.5626

Abstract

Seed-borne fungi are fungi that are associated with seeds and can be a source of plant disease in the field. Therefore, this study aims to utilize DNA barcoding to identify seed-borne fungi isolates from Tanjung Morawa B village. This investigation took place at Tangerang's genetic science laboratory in Tangerang was the site of this investigation. Sequence and phylogenic tree building, PCR amplification with MyTaq HS Red Mix, 2X (Bioline, BIO-25048), and genomic DNA extraction with the Quick-DNA Magbead Plus Kit (Zymo Research, D4082) were the research methods used. The electrophoresis results showed that the DNA made from PCR using Primers ITS-1 and ITS-4 was of high quality. This was clear from the electrophorograms for both the TMIE isolate sample and the control. Using ITS-1 and ITS-4 for DNA amplification produced favorable results, with virtually no smears detected. The Basic Local Alignment and Search Tool (BLAST) program, which is connected to the Genebank database, was employed to analyze the sequence results in the form of a base sequence with a length of 581 base pairs. Based on the genetic similarity results of the isolate's DNA with Genebank, we determined that the TMIE isolate was 100% similar to Curvularia eragrostidis.
Co-Authors Astria Ayu Ramadianti Bonny Poernomo Wahyu Soekarno Chandra Sigalingging Depari, Nelfita Rizka S. Dini Novita Sari DWI SURYANTO Elimasni Emma Fadhilah Emy Hariati Fatimah Pohan Febriantika, Febriantika Florina Yanti, Sisilia Furqan Khalidy Harahap, Siti Nurlani Hariati, Emy Hendra Hendra Hendri Irawan Jairani, Eka Nenni Jane Melita Keliat Juhardi Sembiring Keliat, Jane Melita Khairani, Siti Khairuna Utami Khairuna Utami Khairuna Utami Kusumawati, R Lia Leni Malinda Lily Novianty Lily Novianty Lubis, Ruhilah Malinda, Leni Mardiah Mastari Ramadhani Mirza, Mirzan Hasibuan Muhammad Khaibar Putra Adhitia Nelfita Rizka S Depari Nelfita Rizka S. Depari Nelfita Rizka S. Depari NIZMI PUTRI Novianti, Lily Novianty, Lily Novita Anggraini Nurbaity Situmorang Nuri Ramadhan Nurlani Harahap, Siti Nurliana NURLIANA NURLIANA Nurul Ajimah Ritonga, Merdayana, Sakdan Azwan Nurwijayanti Perry Boy Chandra Siahaan Rahmat Suryanto Pirngadi Ramadhan, Nuri Rara Astili Siregar Riani Sari Sembiring Ritonga, Nurul Ajima Rizka S. Depari, Nelfita Rizky Ananda Saiful Amir Sari Ginting, Makhrani Sari Sembiring, Riani Sari, FItri Dian Nila Sembiring, Juhardi Septiarini Zuliati Sisilia Florina Yanti Sisilia Florina Yanti Siti Khairani Siti Khairani Siti Nurlani Harahap SITI NURLANI harahap Situmorang, Nurbaity Suharsih Suharsih Syarifah Ainun Harahap Titiek Siti Yuliani Tuti Wardani Siregar Tuti Wardani Siregar Utami , Khairuna Utami, Khairuna Virginia Tamara , Chatrine Wahyu Hidayat Wardani Siregar, Tuti Winny Iftar Zuliati, Septiarini