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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Industri Pertanian VALENSI Jurnal Veteriner JURNAL KIMIA SAINS DAN APLIKASI Jurnal Natur Indonesia JURNAL SAINS DAN TEKNOLOGI INDONESIA Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat AGRIVITA, Journal of Agricultural Science SEMIRATA 2015 Biology, Medicine, & Natural Product Chemistry Jurnal Sumberdaya Hayati Jurnal Kimia Mulawarman Indonesia Chimica Acta Science and Technology Indonesia Al-Kimia Indonesian Journal of Chemistry Biosaintifika: Journal of Biology & Biology Education Journal of Tropical Crop Science Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat Jurnal Agrotropika Makara Journal of Science Molekul: Jurnal Ilmiah Kimia Jurna Beta Kimia Jurnal Tumbuhan Obat Indonesia Borneo Journal of Pharmacy Serambi Engineering CEFARS : JURNAL AGRIBISNIS DAN PENGEMBANGAN WILAYAH Jurnal Agribisnis dan Pengembangan Wilayah
Dyah Iswantini
Departemen Kimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Veterinary

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Seleksi Sel Bakteri Dari Minyak Bumi Sebagai Molekul Pengenal Dalam Biosensor Benzena Alfiah Alif; Dyah Iswantini; Henny Purwaningsih; Novik Nurhidayat; Amalyah Febryanti
Al-Kimia Vol 9 No 2 (2021): DESEMBER
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/al-kimia.v9i2.24378

Abstract

Benzene is one of the harmful compounds which can affect both healthcare and environmental quality. Conventionally the effort of detecting this compound still requires several sample pre-treatments, contributing to a long analysis time and sophisticated instrumentation. Therefore, this study was aimed to determine the potency of bacteria as the bioreceptor for detecting benzene electrochemically. The bacteria isolate was immobilized on the working electrode surface. Four bacteria isolates from the petroleum sample were evaluated. The results showed that isolate II produced high oxidation and reduction peak currents as much as 150 µA and -490 µA respectively. The selected bacteria showed characteristics to Pseudomonas sp. physiologically. Since the bacteria can degrade benzene, thus hypothetically it can produce benzene dioxygenase. Through the catechol formation, 3 mM benzene produced 108.7 µA after 11.4 s from the starting scan. This result suggested that the excreted enzyme from the selected bacteria could react with benzene enzymatically.
Ekstrak Sambiloto Menurunkan Patogenesitas Ookista Eimeria Tenella Yulia Yellita; Umi Cahyaningsih; Dyah Iswantini Pradono; Wiwin Winarsih; Wasmen Manalu
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (348.817 KB)

Abstract

Eimeria tenella is one of the nine of Eimeria species, a pathogenic intraseluler protozoa causing aviancoccidiosis. Infection was initiated by the ingestion of sporulated oocysts. The aim of this study was toinvestigate the effect of E. tenella oocyst incubation in methanol extract of Andrographis paniculata beforeinfection in broiler performance. This research used 115 broiler DOC (CP 707) devided into five groups,each group consisted of 23 broilers. The infection with 1x105 oocyst were done at the 14th day old of chicken.The 1st group was placebo (KN), while the 2nd group was infected with unincubated oocyst (KP), and theother three groups i.e. : 3rd, 4th, 5th were infected with incubated oocyst in A. paniculata extract for 2, 4, and6 hours, respectively. The number of oocysts in feces were counted on day 5th to 14th post-infection, theheterophile and macrophages were counted from caecum histology preparation, by slaughtered threechickens of each of groups on the day 0,3,6.9, and 14 post infection, and accretion body weight wasmeasured by weighing chickens per week to five-week old chickens. The results of this study indicated thatthe incubation period the sporulated oocyst in the extract of A.paniculata for six hours before infection,reduced the number of oocysts production in the feces, the number of inflammatory cells (macrophages andheterophile) in the cecum, and increases body weight (gain). In conclusion A.paniculata extract decreasedthe pathogenisity of E.tenella oocyst, so the extract of A.paniculata has good potential as anticoccidia. Itis high likely that A. paniculata extract has a potential to be anticoccidia.
Ekstrak Sambiloto (Andrographis paniculata) Menurunkan Jumlah Skizon, Mikrogamet, Makrogamet, dan Oosista Eimeria tenella (EXTRACT OF ANDROGRAPHIS PANICULATA DECREASED SCHIZONTS, MICROGAMETES, MACROGAMETES AND OOCYSTS NUMBER OF EIMERIA TENELLA) UMI CAHYANINGSIH; RESSY RIANDCI; DYAH ISWANTINI
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.698 KB)

Abstract

The aim of this research was to observe the effect of ethanol extract of Andrographis paniculata givenin grading doses to the schizonts, microgamete, macrogamete, and oocytes counts of Eimeria tenella inchicken caecum. A total of ninety day old broiler chicks were used in the study. At two weeks old the broilerswere divided into six groups. Each group consisted of 15 broilers, the 6 groups were: (i) negative control(broilers did not receive any treatment); (ii) positive control (each animal were infected with 104 E. tenellaoocytes); (iii) medicine control (each animal were infected with 104 E. tenella oocytes and coccidiostat); (iv)A1 (each animal were infected with 104 E. tenella oocytes and paniculata extract 90 mg/kg body weight); (v)A2 (each animal were infected with 104 E. tenella oocytes and paniculata extract 180 mg/kg body weight);and (vi) A3 (each animal were infected with 104 E. tenella oocytes and paniculata extract 360 mg/kg bodyweight). At day 6, 9, 13, 16, and 22 post infection three broilers from each group were sacrificed and theirceca were collected for histopathological examination. The results showed that paniculata extract at dose90 mg/kg body weight and 180 mg/kg body weight was able to decrease the numbers of shizont, microgamete,macrogamete, and oocytes of E. tenella in the chicken caecum.
PENGARUH TINGKAT KEMATANGAN BUAH TERHADAP AKTIVITAS LARVASIDA DAN SIFAT FISIKO-KIMIA MINYAK KAMANDRAH (Croton tiglium L.) NOOR ROUFIQ AHMADI; DJUMALI MANGUNWIDJAJA; ONO SUPARNO; DYAH ISWANTINI P.
Jurnal Penelitian Tanaman Industri Vol 17, No 4 (2011): Desember 2011
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v17n4.2011.163-168

Abstract

ABSTRAKIndonesia terkenal kaya akan keanekaragaman hayati, termasukjenis tumbuhan yang mengandung bahan aktif insektisida. Tanamankamandrah (Croton tiglium L.) merupakan salah satu tanaman obat yangbanyak terdapat di wilayah Indonesia dan telah dimanfaatkan sebagaiinsektisida nabati. Penelitian ini bertujuan untuk mengetahui karakteristikfisiko-kimia minyak kamandrah pada berbagai tingkat kematangan sebagailarvasida nabati terhadap larva nyamuk demam berdarah dengue (A.aegypti). Penelitian dilakukan di Laboratorium Balai Penelitian TanamanRempah dan Aneka Tanaman Industri (Balittri) Sukabumi, Balai BesarLitbang  Pascapanen  Pertanian  Bogor,  Insektarium  LaboratoriumParasitologi dan Entomologi Kesehatan FKH IPB Bogor, dan Biofarmaka-LPPM IPB Bogor, mulai bulan Februari sampai Desember 2010. Minyakkamandrah diperoleh dengan ekstraksi menggunakan pengempa hidrolikpada suhu 65 o C dan tekanan 7,9 MPa pada buah kamandrah dengan tigatingkat kematangan yang berbeda: warna kulit buah hijau kecokelatan,cokelat kehijauan, dan cokelat penuh. Minyak yang diperoleh selanjutnyadianalisis bilangan asam, kadar asam lemak bebas, bilangan peroksida,indeks bias, berat jenis, dan nilai warna minyak, serta uji larvasidaterhadap larva nyamuk A. aegypti instar III. Penentuan nilai lethalconcentration (LC) dilakukan dengan metode probit analisis (FinneyMethod). Hasil penelitian menunjukkan bahwa berdasarkan nilai LC,rendemen, dan mutu minyaknya, buah kamandrah yang berwarna kulitcokelat penuh lebih berpotensi sebagai larvasida nabati terhadap larva A.aegypti. Rendemen minyak kamandrah 20,42% dan nilai LC 50 adalah132,67 ppm (24 jam) dan 70,08 ppm (48 jam). Minyak tersebut memilikibilangan asam 8,76 mg KOH/g minyak; kadar asam lemak bebas 4,36 mgKOH/g minyak; bilangan peroksida 3,59 meq O/100g minyak; indeks bias1,4783; bobot jenis 0,9466 g/ml; dan warna meliputi nilai L* 73,03; a*3,26; dan b* 64,13. Minyak kamandrah berpotensi dapat dimanfaatkansebagai larvasida nabati dalam pengendalian vektor penyakit DBD.Kata kunci : Croton tiglium L., kematangan buah, minyak kamandrah,larvasidaABSTRACTEffect of Maturity Level of Fruits on Larvicidal Activityand Physico-Chemical Properties of Kamandrah (Crotontiglium L.) OilIndonesia is famously rich in biodiversity, including species of plants thatcontain active ingredient for insecticide. Kamandrah plant (Croton tigliumL.) is one of many medicinal plants found in parts of Indonesia and it hasbeen used as an insecticidal plant. The objectives this research were todetermine the physico-chemical characteristics and larvasidal activities ofoil extracted from kamandrah seeds with varying levels of maturity asbotanical larvicide for mosquito dengue fever. The expreriments wereconducted in the Laboratory of Indonesian Spice and Industrial CropsResearch Institute (ISICRI) Sukabumi, Indonesian Center for AgriculturalPostharvest Research  and  Development (ICAPRD),  InsectariumLaboratory of Health Parasitology and Entomology, Faculty of Veterinaryof Bogor Agricultural University (IPB), and Biopharmaca IPB Bogor,from February to December 2010. The oil was obtained through extractionusing hydraulic pressurer at 65 o C temperatures and 7.9 MPa pressures ofkamandrah fruits with three different maturity levels : brownish green,greenish brown, and fully brown color of fruit skins. Oil gained was thenanalyzed its acid number, free fatty acid content, peroxides number,refractive index, specific gravity, and oil color values, as well as the oillarvasida test against mosquito larvae A. aegypti instar III. Determinationthe oil lethal concentration (LC) values was tested using probit analysismethod (Finney Method). The results showed that based on the values ofLC, content, and quality of the oil, full-brown colored kamandrah fruits aremore potential as larvivida against A. aegypti larvae. Oil content of thekamandrah was 20.42% while LC 50 values were 132.67 ppm (at 24 hours)and 70.08 ppm (at 48 hours). The acid number of the oil was 8.76 mgKOH/g oil; free fatty acid level of 4.36 mg KOH/g oil; peroxide number of3.59 meq O/100 g oil, refractive index of 1.4783; density of 0.9466 g/ml;and the color values were 73.03, 3.26, and 64.13 for L*, a*, and b*,respectively. Therefore, kamandrah oil is very potential to be used aslarvicide for controling the vector of dengue disease.Key words: Croton tiglium L., fruit maturity, kamandrah oil, larvicide
FORMULASI LARVASIDA NABATI BERBASIS MINYAK BIJI KAMANDRAH (Croton tiglium L.) TERSTANDAR SEBAGAI PENCEGAH PENYAKIT DEMAM BERDARAH DENGUE Evul Winoto; Dyah Iswantini; Irmanida Batubara; Upik Kesumawati Hadi
Buletin Penelitian Tanaman Rempah dan Obat Vol 24, No 2 (2013): Buletin Penelitian Tanaman Rempah dan Obat
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v24n2.2013.%p

Abstract

ABSTRAKKamandrah (Croton tiglium L.) merupakan tanaman obat yang banyak terdapat di Kalimantan. Biji kamandrah banyak dimanfaatkan sebagai obat pencahar, racun ikan, dan pembunuh jentik nyamuk. Penelitian ini bertujuan mendapatkan formula minyak biji kamandrah untuk larvasida nabati yang efektif, aman dan mendapatkan minyak kamandrah terstandar sebagai bahan baku larvasida nabati. Analisis fisiko kimia minyak biji kamandrah hasil budidaya di Sukabumi memberikan hasil kadar air 0,33%, keasaman 0,09%, viskositas 4,1 cP, berat jenis 0,9425 g ml-1, indeks bias 1,4788 serta kadar asam lemak bebas 1,65%. Hasil uji ini lebih baik dibanding dengan tanpa budidaya dari Kalimantan dan Sukabumi. Uji menggunakan spektrofotometri menunjukkan kandungan piperine minyak biji kamandrah hasil budidaya Sukabumi sebesar 0,046%; tanpa budidaya dari Sukabumi dan Kalimantan masing-masing 0,043% dan 0,037%. Kandungan piperine berpengaruh terhadap hasil uji efikasi larva nyamuk Aedes aegypti instar III, nilai LC50 pengamatan 24 jam minyak kamandrah hasil budidaya Sukabumi sebesar 114,4 ppm, minyak kamandrah tanpa budidaya dari Sukabumi dan Kalimantan masing-masing 125,2 dan 212,9 ppm. Formulasi larvasida metode granulasi basah terhadap minyak biji kamandrah hasil budidaya Sukabumi menunjukkan, kandungan minyak kamandrah 15% dengan emulsifier gom arab memberikan hasil paling efektif dengan nilai LC50 24 jam sebesar 210,01 ppm. Uji stabilitas formula larvasida nabati minyak biji kamandrah yang disimpan pada temperatur 30, 40 dan 50oC selama 28 hari menunjukkan tidak ada perubahan fisik pada granul. Selama penyimpanan terjadi peningkatan kandungan piperine dalam formula larvasida antara 0,6-234%. Uji durabilitas formula larvasida terhadap larva A. aegypti menunjukkan penurunan potensi larvasida sampai di bawah 50% pada hari ke 12 setelah aplikasi. 
Glucose Biosensor Using Selected Indonesian Bacteria DYAH ISWANTINI; NOVIK NOVIK; . TRIVADILA
Microbiology Indonesia Vol. 5 No. 1 (2011): March 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (83.954 KB) | DOI: 10.5454/mi.5.1.2

Abstract

Microbial glucose sensors have been developed using Escherichia coli bacterial strains from Japan. However, there is interest in developing local bacteria as glucose sensors in Indonesia. In this research, the stability and the potential of a selected number of Indonesian bacteria as glucose biosensors was explored. Results of this study indicate that three of them, E. coli, Bacillus subtilis, and Thermus filiformis exhibit properties of high viability and stability at high temperature (30-60 ºC). Spectrophotometrical and electrochemical measurements showed significant absorbance values and highly stable current features for E. coli as indicated by its high capacity to produce glucose dehydrogenase. E. coli, B. subtilis, and T. filiformis produced currents of 3.25 µA, 0.2 µA, and 0.02 µA respectively, and E. coli also produced a much higher activity of glucose dehydrogenase. Electrochemical measurement using E. coli-modified carbon paste electrode allowed the determination of glucose concentration of up to 20 mM. Therefore, Indonesian E. coli has a high stability and can be used as a glucose biosensor
SYNERGISTIC ACTIVITY OF ENZYMES PRODUCED BY EUPENICILLIUM JAVANICUM AND ASPERGILLUS NIGER NRRL 337 ON PALM OIL FACTORY WASTES TRESNAWATI PURWADARIA; NONI NIRWANA; Pius P. KETAREN; DYAH ISWANTINI PRADONO; YANTYATI WIDYASTUTI
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 20 (2003)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2003.0.20.178

Abstract

The use of palm kernel cake (PKC) and palm oil mill effluent (POME), substances from palm oil factory wastes, for monogastric is limited by their high cellulose and mannan contents. Hydrolytic enzymes have been supplemented to increase the nutrient digestibility. The maximal digestibility was obtained in the synergistic action of all enzyme components including B-D-endoglucanase (CMCase), B-D-glucosidase, B-D-mannanase, p-D-mannosidase, and oc-D-galactosidase. Two kinds of enzymes produced by Eupenicillium javanicum  and Aspergillus niger NRRL 337 on the submerged culture containing 3% coconut meal were selected to hydrolyze PKC or dry POME. Enzyme from E. javanicum contained higher CMCase, B-D-mannanase, and a-D-galactosidase activities, while that from  A. niger  NRRL 337 contained more p-D-glucosidase and p-D-mannosidase activities. Saccharification (hydrolytic) activities of enzyme mixtures on PKC and POME were determined at pH 5.0, the optimal pH for p-D-mannanase from  E. javanicum,  and at 5.4 the optimal pH for a-D-galactosidase from  E. javanicum  and P-D-glucosidase from  A. niger NRRL 337. The enzyme proportions of E. javanicum and A. niger NRRL 337 were 100 : 0, 80 : 20, 60 : 40, 40 : 60, and 0 : 100%. The highest Saccharification activity on both substrates was observed on the mixture of 80% A. niger NRRL 337. The pH levels did not significantly affect Saccharification activity. Fiber components in PKC were more digestable than in POME. Further analysis on the reducing sugar components using thin layer chromatography showed that more monomers were produced in the 60 or 80% of A. niger NRRL 337. The glycosidases of A. niger NRRL 337 played more important role in the Saccharification activity. Keywords:        Synergistic activity/ palm kernel cake/palm oil mill effluent/ Eupenicillium javanicum/ Aspergillus niger NRRL 337
Voltammetric Detection of Uric Acid at Screen Printed Electrode: A Review Wulan Tri Wahyuni; Dyah Iswantini Pradono; Selvitia Ulandari
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 14, No 2: December 2021
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Uric acid is a compound produced from purine metabolism. The excess amount of these compounds could causes diseases in the body. Effective and practical methods are needed for early detection of uric acid. Technically, uric acid could be detected using enzymes (enzymatic) and without enzymes (non-enzymatic). Several methods have already reported for uric acid detection in biological samples, including fluorimetry, colorimetry, liquid chromatography, flow injection analysis, chemiluninescence, and electrochemical detection. Among electrochemical detection methods, voltammetry is one of the most prospective for uric acid detection. This review covers the approach of uric acid detection including enzymatic and nonenzymatic approach, several voltametric technique for uric acid detection, and screen printed and modified electrode as platform for uric acid detection. Analytical performance such as linear range, sensitivity, selectivity and reproducibility of uric acid detection using various voltametric method and at different platform were also highlighted.
Essential Oil Components, Metabolite Profiles, and Idioblast Cell Densities in Galangal (Kaempferia galanga L.) at Different Agroecology Subaryanti Subaryanti; Yohana Caecilia Sulistyaningsih; Dyah Iswantini; Triadiati Triadiati
AGRIVITA, Journal of Agricultural Science Vol 43, No 2 (2021)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v43i2.2631

Abstract

Galangal is widely cultivated for the multifunctional plant. This study analyzed the essential oil chemical components, metabolite profiles, and idioblast cell densities in seven galangal accession grown at two different altitudes (locations A and B). The galangal accessions included: Purbalingga, Cilacap, Purworejo, Karanganyar, Pacitan, Madiun, and Galesia 2 which was used as the control. The results showed that the highest essential oil content was obtained from MAD (3.22%) at location A. The highest levels of ethyl-p-methoxycinnamate (EPMC) were obtained from PBG (74.8%) at location B and PCT (71.6%) at location A. The metabolite profiles of the galangal rhizomes were divided into two clusters based on the metabolite content. The first cluster had one accession: PCT from location A with genkwanin as the metabolite marker. The second cluster consisted of CLP from location A and PBG, MAD, and GAL2 from location B. The highest density of idioblast cells was found in PCT (90.5 cells/mm2) at location A and PBG accessions (77.1 cells/mm2) at location B. The PBG and PCT accessions can be recommended as a candidate of superior varieties based on their high EPMC content.
PENGARUH TINGKAT KEMATANGAN BUAH TERHADAP AKTIVITAS LARVASIDA DAN SIFAT FISIKO-KIMIA MINYAK KAMANDRAH (Croton tiglium L.) NOOR ROUFIQ AHMADI; DJUMALI MANGUNWIDJAJA; ONO SUPARNO; DYAH ISWANTINI P.
Jurnal Penelitian Tanaman Industri Vol 17, No 4 (2011): Desember 2011
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v17n4.2011.163-168

Abstract

ABSTRAKIndonesia terkenal kaya akan keanekaragaman hayati, termasukjenis tumbuhan yang mengandung bahan aktif insektisida. Tanamankamandrah (Croton tiglium L.) merupakan salah satu tanaman obat yangbanyak terdapat di wilayah Indonesia dan telah dimanfaatkan sebagaiinsektisida nabati. Penelitian ini bertujuan untuk mengetahui karakteristikfisiko-kimia minyak kamandrah pada berbagai tingkat kematangan sebagailarvasida nabati terhadap larva nyamuk demam berdarah dengue (A.aegypti). Penelitian dilakukan di Laboratorium Balai Penelitian TanamanRempah dan Aneka Tanaman Industri (Balittri) Sukabumi, Balai BesarLitbang  Pascapanen  Pertanian  Bogor,  Insektarium  LaboratoriumParasitologi dan Entomologi Kesehatan FKH IPB Bogor, dan Biofarmaka-LPPM IPB Bogor, mulai bulan Februari sampai Desember 2010. Minyakkamandrah diperoleh dengan ekstraksi menggunakan pengempa hidrolikpada suhu 65 o C dan tekanan 7,9 MPa pada buah kamandrah dengan tigatingkat kematangan yang berbeda: warna kulit buah hijau kecokelatan,cokelat kehijauan, dan cokelat penuh. Minyak yang diperoleh selanjutnyadianalisis bilangan asam, kadar asam lemak bebas, bilangan peroksida,indeks bias, berat jenis, dan nilai warna minyak, serta uji larvasidaterhadap larva nyamuk A. aegypti instar III. Penentuan nilai lethalconcentration (LC) dilakukan dengan metode probit analisis (FinneyMethod). Hasil penelitian menunjukkan bahwa berdasarkan nilai LC,rendemen, dan mutu minyaknya, buah kamandrah yang berwarna kulitcokelat penuh lebih berpotensi sebagai larvasida nabati terhadap larva A.aegypti. Rendemen minyak kamandrah 20,42% dan nilai LC 50 adalah132,67 ppm (24 jam) dan 70,08 ppm (48 jam). Minyak tersebut memilikibilangan asam 8,76 mg KOH/g minyak; kadar asam lemak bebas 4,36 mgKOH/g minyak; bilangan peroksida 3,59 meq O/100g minyak; indeks bias1,4783; bobot jenis 0,9466 g/ml; dan warna meliputi nilai L* 73,03; a*3,26; dan b* 64,13. Minyak kamandrah berpotensi dapat dimanfaatkansebagai larvasida nabati dalam pengendalian vektor penyakit DBD.Kata kunci : Croton tiglium L., kematangan buah, minyak kamandrah,larvasidaABSTRACTEffect of Maturity Level of Fruits on Larvicidal Activityand Physico-Chemical Properties of Kamandrah (Crotontiglium L.) OilIndonesia is famously rich in biodiversity, including species of plants thatcontain active ingredient for insecticide. Kamandrah plant (Croton tigliumL.) is one of many medicinal plants found in parts of Indonesia and it hasbeen used as an insecticidal plant. The objectives this research were todetermine the physico-chemical characteristics and larvasidal activities ofoil extracted from kamandrah seeds with varying levels of maturity asbotanical larvicide for mosquito dengue fever. The expreriments wereconducted in the Laboratory of Indonesian Spice and Industrial CropsResearch Institute (ISICRI) Sukabumi, Indonesian Center for AgriculturalPostharvest Research  and  Development (ICAPRD),  InsectariumLaboratory of Health Parasitology and Entomology, Faculty of Veterinaryof Bogor Agricultural University (IPB), and Biopharmaca IPB Bogor,from February to December 2010. The oil was obtained through extractionusing hydraulic pressurer at 65 o C temperatures and 7.9 MPa pressures ofkamandrah fruits with three different maturity levels : brownish green,greenish brown, and fully brown color of fruit skins. Oil gained was thenanalyzed its acid number, free fatty acid content, peroxides number,refractive index, specific gravity, and oil color values, as well as the oillarvasida test against mosquito larvae A. aegypti instar III. Determinationthe oil lethal concentration (LC) values was tested using probit analysismethod (Finney Method). The results showed that based on the values ofLC, content, and quality of the oil, full-brown colored kamandrah fruits aremore potential as larvivida against A. aegypti larvae. Oil content of thekamandrah was 20.42% while LC 50 values were 132.67 ppm (at 24 hours)and 70.08 ppm (at 48 hours). The acid number of the oil was 8.76 mgKOH/g oil; free fatty acid level of 4.36 mg KOH/g oil; peroxide number of3.59 meq O/100 g oil, refractive index of 1.4783; density of 0.9466 g/ml;and the color values were 73.03, 3.26, and 64.13 for L*, a*, and b*,respectively. Therefore, kamandrah oil is very potential to be used aslarvicide for controling the vector of dengue disease.Key words: Croton tiglium L., fruit maturity, kamandrah oil, larvicide
Co-Authors , Triadiati . TRIVADILA Adayani Nurjayanti Adelia, Nesha Adi Riyadhi Adiarso Afifi, Muhammad Ridho Agus Kartono Agus Sudiman Tjokrowardojo, Agus Sudiman Ahmad Sjahriza Ahmad, Shahrul Nizam Ai Susanti Akhiruddin Maddu Akmalina, Rifkah Alfiona, Della Ali Aulia Ghozali Ali Aulia Ghozali Alif, Alfiah Amalyah Febryanti Amalyah Febryanti Ana Fitriyani Anggia Murni Aninda, Yunita Anja Meryandini Bagaskoro Tuwalaid Budi Riza Putra Charlena Deden Saprudin Diah Ratnadewi Dian Siska RF Dinda Iryawati Dita Ariyanti Djumali Mangunwidjaja DJUMALI MANGUNWIDJAJA Dwiastuti, Rahma Eka Mardiah ELIZABETH MARTATILOFA Eti Rohaeti Euis Filaila Evul Winoto Evul Winoto Fadillah, Wendi Nurul Firdaus, Ridwan Fitriani Indahsari Franco, Christopher MM Fretty Yurike Fyrda Shellia Ghozali, Ali Aulia Hafshah, Nurul Haki, Horiana hanifah hanifah Harry Noviardi Hasim - Hefni Effendi Henny Purwaningsih Ida Ayu Putu Sri Widnyani Irmanida Batubara Ismet, Rhahmasari Joni Prasetyo, Joni Jovianto, Andrian Karnadi Komar Sutriah Kurniawan, Yehezkiel Steven Latifah K Darusman latifah K Darusman Latifah K. Darusman LATIFAH KOSIM DARUSMAN LATIFAH KOSIM DARUSMAN Lawa, Yosep Lenni Fitri Lenni Fitri Lestarani, Dewi M. Rafi Mahat, Mohd Muzamir Mardiana, Eni Mashuri Waite Maulidiyah Utami Muammar Yulian Naat, Johnson N. Nampiah Sukarno Neolaka, Yantus A B Nisa, Ulfiatun NONI NIRWANA Noor Roufiq Ahmadi Novia Rahma NOVIK NOVIK Novik Nurhidayat Novik Nurhidayat Novik Nurhidayat Novik Nurhidayat Novik Nurhidayat Novik Nurhidayat Novik Nurhidayat Novriyandi Hanif Nurdiani Nurdiani Ono Suparno Pius P. KETAREN Purbowatiningrum Ria Sarjono Purwantiningsih Sugita Putra, Sukma Triperdana Rahminiwati, Min Rani, Ismarani RESSY RIANDCI RHOITO FROISTA SILITONGA Rosihan Rosman Rosihan Rosman Rudi Heryanto Rut Novalia Rahmawati Sianipar Sagala, Zuraida Sandra Arifin Aziz Selvitia Ulandari Setyanto Tri Wahyudi Sianipar, Rut Novalia Rahmawati Sri Mulijani Subaryanti Subaryanti Subaryanti Subaryanti Subaryanti Sulitijorini, Sulitijorini SUMINAR SETIATI ACHMADI Taopik Ridwan Tatik Khusniati TRESNAWATI PURWADARIA Tuti Hayati Ramdhani Umi Cahyaningsih Upik Kesumawati Hadi UTUT WIDYASTUTI Waite, Mashuri Wasmen Manalu Wei Zhang Wendi Nurul Fadillah Wiwin Winarsih Wulan Tri Wahyuni YANTYATI WIDYASTUTI Yohana C. Sulistyaningsih Yohana Caecilia Sulistyaningsih Yulia Yellita YULIN LESTARI