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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Veteriner BIOTROPIA - The Southeast Asian Journal of Tropical Biology Gadjah Mada International Journal of Business Journal of Tropical Biodiversity and Biotechnology Science and Technology Indonesia Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Riset Akuakultur JSFK (Jurnal Sains Farmasi & Klinis) Jambura Journal of Chemistry Majalah Farmasetika JURNAL KAJIAN VETERINER Narra J Makara Journal of Science Gunung Djati Conference Series Jurnal Kedokteran Hewan Current Biomedicine Policy Brief Pertanian, Kelautan, dan Biosains Tropika Borneo Journal of Pharmacy Berita Biologi

Huda Shalahudin Darusman

IPB University

Author-ID : 2938356

Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Perbandingan Nilai Kardiorespirasi dan Suhu Tubuh Dugong Dewasa dan Bayi COMPARISON OF CARDIORESPIRATION RATES AND BODY TEMPERATURE IN ADULT AND BABY DUGONG Agik Suprayogi; Sumitro -; Megawati Iskandar; Rika Sudranto; Huda Salahudin Darusman
Jurnal Veteriner Vol 8 No 4 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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A study to compare the physiological values (heart beat and respiration rate, body temperature, and electrocardiogram/ECG) between adult dugong (AD) and baby dugong (BD) was carried out. The dugong’s physiological values were examined by patient monitor. The results showed that heart beat rates of baby dugong (87.11+8.70 per minute) was higher than in adult dugong (45.73+4.10 per minute), but no significant difference was observed on the respiration rates between baby dugong (12.89+3.08 per minute) and adult dugong (10.11+3.44 per minute). The body temperature of baby dugong (29.26+0.77oC) was lower than that of adult dugong (31.42 + 0.41) oC. Regular heart beat rhythms was detected on Lead I, II, and III of ECG of both in baby and adult dugong. The ECG values of baby dugong was higher on voltages and shorter in duration (p<0.05) as compared than those of adult dugong. The axis values in adult dugong showed a position more on the right by (+ 90o)–(- 135o) with mean electricity axis (MEA) of +157,5o compared than axis value in baby dugong which appeared to be more on the left by (-32.7o)–(-76.7o) with mean MEA of -54.7o. The differences in cardiorespiration and body temperature values between adult dugong and baby dugong appeared to be due to differences in ages of the mammal, especially related with the differences on the metabolic rate and anatomical growth.

Anatomi Makroskopis dan Analisis Dinamika Profil Darah pada Proses Penyembuhan Luka pada Kulit Landak Jawa (Hystrix javanica) (MACROSCOPIC ANATOMY AND HEMATOLOGICAL CHANGES DURING WOUND HEALING PROCESS IN THE SKIN OF THE JAVA PORCUPINE (Hystrix javanica)) Srihadi Agungpriyono; Andhika Yudha Prawira; Huda Salahudin Darusman; Wartika Rosa Farida; Savitri Novelina
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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In Sunda Porcupine, wound healing occurred in a relative quick period, minimum scar formation, and without infection even though the wound is large and severe. The physiology which involved blood function as the systemic component is important in wound healing. This study aimed to acknowledged the macroscopic anatomy of the clinical signs, the role of blood component, and blood biochemistry in wound healing in Sunda porcupine (Hystrix javanica). The study used 6 adults of Sunda porcupine (3 males and 3 females). Stadium 3 wounds (full-thickness wound) were applied in thoracodorsal and lumbosacral region, approximately 10-11 cm2. Clinical signs observations were performed in day 0, 3, 7, 10, 14, 30, and 40, while blood collection were conducted in day 0, 7, 14, 21, 30, and 40 post wounding. The results of this study showed that wound closure occurred in 30-40 days post wounding with thoracodorsal wound closed faster than that in lumbosacral. Blood profile alteration during wound healing showed that wound healing occurred well. The increasing profile of erythrocytes and platelets during wound healing showed that these two components might become important of internal factor in wound closure, whereas the alteration of leucocytes showed no prolonged inflammation in wound healing in Sunda porcupine. The alteration of liver enzyme and renal function showed a fluctuation however the wound healing occurred well, whereas the decreased blood glucose level indicated no prolonged wound healing to chronic stage. These results give the information of clinical view and internal factors which contribute to the wound healing in the skin of Sunda porcupine.

Jamu Pelangsing Galian Singset Bermanfaat Sebagai Antiobesitas dan Antilipidemia pada Tikus (JAMU GALIAN SINGSET USEFUL AS AN ANTIOBESITY AND ANTILIPIDAEMIA IN RATS) Ice Lusia Marta; Huda Shalahudin Darusman; Damiana Rita Ekastuti
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Obesity and dyslipidemia are health problems that can increase the risk of cardiovascular disease. This study aims to analyze the potency of antiobesitas and antidislipidemia of jamu galian singset (JGS). The research method used complete randomized design with 8 treatment groups and 4 replications as follows: P1 (Standard Feed + Aquadest), P2 (Standard Feed + 200 mg Orlistat / kg BW), P3 (Standard Feed + 176,4mg JGS / 200g BW) P4 (Standard Feed + 352,8mg JGS / 200 g BW), P5 (High Fat Feed + Aquadest), P6 (High Fat Feed + 200 mg Orlistat / kg BW), P7 (High Fat Feed + 176,4 mg JGS / 200 g BW) and P8 (High Fat Feed + 352,8 mg JGS / 200 g BW). The parameters observed were feed intake, body weight gain, body weight gain ratio, fat intake, fat absorption, fat digestibility, feces fat, abdominal fat, muscle fat, body weight, body fat percentage and lipid profile, include Cholesterol total (TC), triglycerides (TG), HDL and LDL. The results showed that JGS significantly (P <0,05) decreased the body weight gain of the rat, the ratio of body weight gain per feed intake and the weight of abdominal fat. Giving JGS increases the absorption of fat, but not found high fat in the feces. Jamu galian singset has no effect (P> 0.05) on rat lipid profile. Baced on the results of this study it can be concluded that JGS can be used as antiobesity and more effectively decrease body weight gain on high fat diet than standard feeding diet. Jamu galian singset are suspected to have a different working mechanism with orlistat as antiobesitas.

NILAI NORMAL ELEKTROKARDIOGRAM, FREKUENSI JANTUNG, RESPIRASI DAN SUHU TUBUH DUGONG DUGON NORMAL VALUES OF ELECTROCARDIOGRAM, HEART RATE, RESPIRATION RATE AND BODY TEMPERATURE OF DUGONG DUGON Agik Suprayogi; Sumitro -; Linda Tjhin; Rika Sudranto; Huda Salahudin Darusman
Jurnal Veteriner Vol 8 No 1 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Dugong dugon yang merupakan mamalia laut, terdaftar sebagai spesies langka yang dilindungi. Sampai saat ini, belum banyak laporan yang tersedia tentang nilai fisiologis normal dari mamalia ini, sehingga menyulitkan dokter hewan dalam memeriksa dan mendiagnosis penyakit pada hewan ini. Oleh karena itu dilakukan studi tentang nilai normal elektrokardiogram (EKG), frekuensi jantung, respirasi, dan suhu tubuh dari seekor Dugong dugon dengan menggunakan alat pemantau pasien (Patient monitor). Parameter yang diukur adalah frekuensi jantung, respirasi, suhu tubuh, dan nilai EKG: gelombang P, komplek QRS, interval PR, interval QT, dan mean electricity axis (MEA). Hasil perekaman menunjukkan bahwa nilai normal frekuensi jantung, respirasi dan suhu tubuh berturut-turut adalah (45,73+4,10) denyut/menit, (10,11+3,44) inspirasi/menit, dan (31,42+0,41)0C. Tidak ada perbedaan yang nyata antara nilai EKG normal pada hantaran I (dada kanan) dan hantaran II. Sementara itu, pada hantaran III menunjukkan nilai voltase yang isoelektris (0 mV). Pada Lead II, gelombang P menunjukkan durasi (0,05+0,02) detik dengan voltase (0,03+0,01) mV, gelombang R menunjukkan voltase (0,07+0,02) mv dengan durasi QRS komplek (0,06+0,01) detik, interval PR (0,26+0,01) detik, interval QT (0,21+0,11) detik, axis jantung (MEA: +90 sampai -135 ) condong ke kanan, dengan irama jantung reguler. Nilai normal Dugong dugon ini menunjukkan karakteristik yang spesifik dibanding nilai normal pada anjing, hal ini mungkin karena adana perbedaan ekologis, struktur anatomi, dan ketebalan dinding thoraks pada kedua hewan tersebut.

SEKUENSING GEN MCR-1 DARI ESCHERICHIA COLI DAN SALMONELLA ENTERITIDIS RESISTAN KOLISTIN Maria Fatima Palupi; Ernes Andhesfa; Hera Maheshwari; Huda Salahuddin Darusman; Etih Sudarnika; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Gen mobilized colistin resistance (mcr) merupakan gen resistan kolistin sulfat yang bisa dipindahkan melalui materi genetik bergerak misalnya plasmid. Tujuan dari penelitian ini adalah mengetahui jenis plasmid gen mcr-1 yang berhasil dipindahkan dari satu isolat Escherichia coli resistan kolistin ke Salmonella Enteritidis ATCC 13076 (dua isolat), dan satu isolat E. coli O157:H7 resistan kolistin positif gen mcr-1. Sekuensing gen mcr-1 kemudian dianalisis di unit Biotek Balai Besar Pengujian Mutu dan Sertifikasi Obat Hewan dengan menggunakan basic local alignment search tools (BLAST) dan software MEGA7. Hasil sekuensing gen mcr-1 dengan panjang nukleotida 309 memiliki homologi tinggi dengan data referen dari Genbank pada gen mcr-1 yang terdapat dalam plasmid. Hasil sekuensing DNA gen mcr-1 sampel lebih cenderung termasuk pada tipe plasmid IncI2 atau IncHI2. Hal ini menunjukkan bahwa resistansi kolistin merupakan ancaman yang nyata karena gen resistan mcr-1 yang ada di Indonesia benar berada di plasmid sehingga mudah disebarkan ke bakteri lain. Kata kunci: mcr-1, plasmid, resistansi ABSTRACT The mobilized colistin resistance (mcr) gene is a colistin sulfate resistance gene that can be transferred through mobile genetic material such as plasmids. The purpose of this study was to determine the type of plasmid mcr-1 gene that was successfully transferred from an isolate of colistin-resistant Escherichia coli to Salmonella Enteritidis ATCC 13076 (two isolates), and one colistin-resistant E. coli O157: H7 that positive mcr-1 gene. The sequencing of the mcr-1 gene was then analyzed in the Biotek unit at National Veterinary Drug Assay Laboratory using basic local alignment search tools (BLAST) and MEGA7 software. The sequencing of the mcr-1 gene with nucleotide length 309 has a high homology with the reference data from Genbank on the mcr-1 gene contained in the plasmid. DNA sequencing of mcr-1 gene samples is more likely to belong to the IncI2 or IncHI2 plasmid types. This shows that colistine resistance is a real threat because the mcr-1 resistance gene in Indonesia is actually in the plasmid so it is easily spread to other bacteria. Keywords: mcr-1, plasmid, resistance

Studi Karakteristik Tipe Diabetes pada Tikus (Rattus novergicus) yang Diinduksi Deksametason (CHARACTERISTIC STUDY OF THE TYPE OF DIABETES IN RAT (RATTUS NOVERGICUS) MODEL INDUCED BY DEXAMETHASONE) Rahmania Hanim; Huda Shalahudin Darusman; Min Rahminiwati
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Diabetes is marked by hyperglicemia with severe symptoms included polydipsi, polyuri, polyphagia,weight loss, and decreased of insulin sensitivity index specially at type 2 diabetes mellitus. The objective of this research was to analyze the characteristics of diabetes mellitus in rat (Rattus novergicus) which induced by dexamethason. A total of 24 rats were divided into four groups which divided as follow: (1) aquabidest subcutaneously (K0), (2) subcutaneous dexamethasone 1 mg/kgBW daily (K1), (3) subcutaneous dexamethasone 2.5 mg/kgBW daily (K2) and subcutaneous dexamethasone 5 mg/kgBW daily (K3). The data of fasting blood glucose, fasting blood insulin, body weight and feed intake, urine volume and water intake collected at day 0, 5, 10 and 15 (five days after the last treatment). The result showed that there was a significant elevation of glucose level followed by significant decreased of insulin sensitivity index and the body weight of rats in group wich induced by dexamethasone but there was no change in feed intake, water intake, volume of urine and fasting blood insulin before and after the treatment. There was insignificant different of total beta pancreatic islet between treatment and control grup. Induction of dexamethasone in mice causes reversible hyperglycemia and has not met the characterisics of either type 1 or 2 diabetes mellitus models. The profile of dexamethasone induced diabetes model has their own spesific type.

Refinement of methodology and deep computational analysis of the thermal images for better estimates of pregnancy diagnosis in cynomolgus monkeys (Macaca fascicularis) Huda Shalahudin Darusman; Sony Hartono Wijaya; Ahmad Kamal Nasution; Entang Iskandar; Dondin Sajuthi
Jurnal Veteriner Vol 22 No 4 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The current use of thermal imaging has been documented in wild animals due to the benefit for having real-time results with less or almost no restrain or invasive methods required - and this is significant for better well-being. This paper will explore the thermal imaging studies as a part of employing non-invasive methods in evaluating physiological function, in particular with refinement of the methods, followed by further computational analysis of the images to ensure the validity of the methods as predictive tools for pregnancy diagnosis. We conducted refinements in thermal imaging methods and computational analysis of deep learning for pregnancy diagnosis of cynomolgus monkeys (Macaca fascicularis) at breeding facility of The Primate Research Center, LPPM IPB University. Subjects were already identified by ultrasound as pregnant in 80, 120 and 130 days. Thermal images along with the temperature data were obtained from FLIR ONE camera in sedated animals with dorso-ventral recumbence. The temperature data were analyzed with linear regression to correlate the skin temperature and the days of pregnancy to make a prediction of pregnancy days based on temperature data. There is a positive correlation of the temperature to the pregnancy days with a function of temperature to days. Further computational analysis of the thermal image, the results showed that the refined methods and the computational analysis brought better interpretation to evaluate health and reproductive status, in particular with the pregnancy diagnosis.

Gambaran Leukosit Setelah Pemberian Nanoenkapsulasi Andaliman (Zanthoxylum acanthopodium DC.) pada Burung Puyuh Pascainduksi Imunosupresan Deksametason Rasyida Ulfa; Akhiruddin Maddu; Huda Salahuddin Darusman; Koekoeh Santoso
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The use of AGP (Antibiotic Growth Promoter) in animal has been banned because of the risk of antibiotic resistance. As an alternative for growth promoter. Andaliman (Zanthoxylum acanthopodium DC.) were used as feed additives. Andaliman is an herbal plant which proven has an immunomodulatory effect. However, herbal plants have low bioavailability in the body, so we need to increase its bioavaibility by reducing particle size using nanoparticle technology. The aim of our study was to determine the effects of three forms of andaliman as extracts, nanoparticles and nanoencapsulation on leukocyte differential, leukocyte count and Heterophil (H) / Lymphocyte.(L) ratio index. In this study we used 24 quails, which is divided into 4 groups, consist of.4 birds each. Group K was control group, birds were given dexamethasone (immunosuppressant), group P1 was given dexamethasone and andaliman extract, group P2 was given dexamethasone and andaliman nanoparticles, whilst group P3 was given dexamethasone and andaliman nanoencapsulation. The results showed that andaliman consumption had a significant effect (P <0.05) on differential leukocytes, number of leukocytes and H / L ratio after induction with immunosuppressants. Total leucocytes count based on the given treatment were K (8080 cell/mm3), P1 (20040 cell/mm3), P2 (20440 cell/mm3), and P3 (22040 cell/mm3). Each mean lymphocytes were K (44,6%), P1 (53,6%), P2(57,4%), and P3 (65,8%). Each mean heterophile were K (50,6%), P1 (41,8%), P2 (37,8%), and P3 (28,6%). Each mean monocytes were K (2,8%), P1 (4,6%), P2(4,8%), and P3 (5,0%). Each mean ratio index Heterophile (H) / Lymphocyte.(L) were K (1,1), P1 (0,7), P2(0,6), and P3 (0,5). It could be concluded that administration of Andaliman extract, Andaliman nanoparticles and Andaliman nanoencapsulation can increase the number of leukocytes, differential leukocytes and decrease the H / L ratio.

Teknik Penanganan Kendali Hewan Sesuai Kaidah Kesejahteraan Hewan Meningkatkan Akurasi Pengukuran Profil Hemodinamika Tikus Laboratorium Huda Shalahudin Darusman; Setyo Widi Nugroho; Fauzi Abdurrahman Munggaran; Dondin Sajuthi
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Chronic discomfort potentially occurred during a hemodynamic measurements in a rat-tail blood pressure chamber due to the animal is restrained in restricted space for a long period – it took 10 repetitions each at approximately 1.5 minutes for a session of hemodynamic measure. Procedures related to the animal welfare methods are required to improve the accuracy of the measurement which would altered from the discomfort to comfort state of the animal. Welfare procedures are represented by humane handling and habituation of the subject (rats) to the operator and the hemodynamic apparatus. Seven rats were subjected in this study and measured for hemodynamic value in three times point: before (T0), after humane handling (T1) and after human handling and habituation to the measurement apparatus (T2), respectively. Six out of seven subjects showed a significant lowering deviation for all variables of hemodynamic measurements, and that deviation followed by a declining trend from T0 to T2, i.e T0>T1>T2. The results indicated that welfare-based approach would benefit the animals from feeling discomfort and yet improving the accuracy and quality of the outcomes.

Co-Authors A.S. Satyaningtijas Achmad, Yusuf Farid Adi Winarto Adi Winarto Agik Suprayogi Ahmad Kamal Nasution Ahmad Subhan Ai Hertati, Ai Akhiruddin Maddu Andhika Yudha Prawira Andi Hiroyuki Angela Mariana Lusiastuti Anita Esfandiari Annisa Dewi, Fitriya Nur Apon Zaenal Mustopa Aprilyana, Lia Ardiansyah Nurdin Benvika Chairunnisa, Sheila Damiana Rita Ekastuti Dayana, Hepy Dewi Ratih Agungpriyono Dewi, Fitriya Nur Annisa Dondin Sajuthi DYAH PERWITASARI -FARAJALLAH Ekawati, Nurlaili Ekowati Handharyani Elok Budi Retnani Entang Iskandar Entang Iskandar Entang Iskandar Entang Iskandar Ernes Andhesfa Etih Sudarnika Fadla, Ilma Nadya Fauzi Abdurrahman Munggaran Femke Den Haas Ferdinandez, Mariana Firdaus, Moh Egy Rahman Fitri, Arni Diana Habib Ilham Maulana Al Adhim Hana Intishar Sawitri Hardo Basuki Harrold Halilintar MR Subu Taopan Hasya, Karin katina HERA MAHESHWARI Hidayaturrahmah Hidayaturrahmah I wayan Teguh Wibawan Ice Lusia Marta IETJE WIENTARSIH Ikramullah, Muh. Chaeril Indra Bachtiar Indra Bachtiar Indriawati, Iin Iqbal Ngabdusani Irawan, Herman Irawan, Shasmita Irma Herawati Suparto Jendri Mamangkey Joko Pamungkas Jusak Nugraha Kanti Rahmi Fauziyah Katrin Roosita koekoeh santoso Krisnayanti, Ni Putu Eka Lina Elfita Lina Elfita Linda Tjhin Lis Rosmanah Loe, Fhady Risckhy Loes (ML) Schure Maria Fatima Palupi Maria Fatima Palupi Maria Fatima Palupi Megawati Iskandar Mia Audina Muhammad Hamdan Mustopa, Apon Z. Ningrum, Emilna Mega Novi Mayasari Noviana, Rachmitasari Novianti, Ela Novriyandi Hanif Nugroho, Setyo Widi Nurfatwa, Maritsa Perdhana, Ika Satya Permanawati Permanawati PERMANAWATI, PERMANAWATI Puji Rianti Putri, Indah Aprianti Rachmitasari Noviana Rahmania Hanim Rahminiwati, Min Randall C. Kyes Rasyida Ulfa Ratna, Lita Tri Ratni Ernita Ratu, Safira Pinaka Pramestika Raymond R. Tjandrawinata Ridzki M.F. Binol Rifqiyah Nur Umami, Rifqiyah Nur Rika Sudranto Rizal, Muhammad Farid Rizki Aulia Ansari Roostita L Balia Rosmanah, Lis Rosyid, Bahaudien Sari, Isti Kartika Savitri Novelina Sela Septima Mariya Setyawaty, Dyah Setyo Widi Nugroho Sibit, Denniswara Silmi Mariya SILMI MARIYA Silmi Mariya Silvy Thiyana Soedarso, Yovi Alwi Sony Hartono Wijaya Sri Wahyuni Srihadi Agungpriyono Subu Taopan, Harrold Halilintar MR Suduri, Adam Sukenda Sukenda Sumitro - Sundari, Betty Suparto, Irma H. SURYA, MARYATI Suryo Saputro Tambunan, Anggitha Tatang Mitra Setia Tatik Mufidah Tommy Langgeng Abimanyu Tri Wibawa Uus Saepuloh UUS SAEPULOH Uus Saepuloh Vinka Aftinata Kusumaputri Wartika Rosa Farida Wasmen Manalu WIDANARNI WIDANARNI Wira, Dwi Wahyuda Yuliana Yuliana Yuliana Zaelani, Bella Fatima Dora

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