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Supplementation of Fermented Moringa Leaves in Feed on Blood Glucose of Striped Catfish (Pangasianodon hypophthalmus) Infected with Aeromonas hydrophila Windarti Windarti; Irwan Effendi; Ronal Kurniawan; Mega Novia Putri; Dimas Gusriansyah; Okta Rizal Karsih
Jurnal Natur Indonesia Vol. 22 No. 2 (2024): October
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.22.2.92-99

Abstract

Moringa leaves can be used as an herbal plant in fish growth to prevent and treat bacterial infections. This study aims to determine the effect of fermented moringa leaves through feed on blood glucose in striped catfish (Pangasianodon hypophthalmus). This research was conducted from September to November 2023 at the Marine Biotechnology Laboratory, Faculty of Fisheries and Marine Sciences, Riau University. The method used is an experimental method using a completely randomized design with 4 (four) treatments and 3 (three) replicates; the treatment dose of fermented moringa leaves is T0 (control), T1 (10 g/kg feed), T2 (15 g/kg feed), and P3 (20 g/kg feed). The catfish measured 5.00 ± 1.00 cm and weighed 4.00 ± 0.50 g, reared in an 80 L tank at a stocking density of 1 fish / 4 L water. The maintenance was carried out for 60 days, and blood glucose observations were made at the beginning (H-1), day 30 (H-30), day 60 (H-60), and post-challenge test (H-75). The results showed that adding fermented moringa leaves to the diet did not affect the blood glucose of the catfish (p>0.05). This indicates that moringa leaves can keep the fish in normal condition after being challenged with Aeromonas hydrophila bacteria
Effect of Different Salinity on the Growth of Chlorella sp in Laboratory Scale Culture Irwan Effendi; Mega Novia Putri; Ulfi Laili Astika; Ronal Kurniawan
Jurnal Natur Indonesia Vol. 22 No. 1 (2024): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.22.1.21-27

Abstract

This study aimed to determine the optimal salinity in increasing the growth rate of Chlorella sp in laboratory scale culture. This research was conducted in July 2023 at the Marine Biotechnology Laboratory, Faculty of Fisheries and Marine Sciences, Universitas Riau. The experimental method was applied using a complete randomized design (CRD) with four treatments and three replicates. The treatments were as follows: T0 (control), T1 (25 ppt salinity), T2 (30 ppt salinity), and T3 (35 ppt salinity). The initial density of Chlorella sp was 250x103 cells/mL, and cell abundance observations were carried out for 14 days. The parameters observed were cell abundance, specific growth rate, and water quality (temperature and pH). The results showed that different salinity influenced the growth of Chlorella sp. 35 ppt salinity gave the best results on cell abundance of 266.66x104 cells/mL, with peak population occurring on day 10. Water quality during the study was still in the normal range and can be tolerated for the growth of Chlorella sp, and the temperature ranged from 25-31oC and pH 6.4-8.0.
Antibacterial activity of Rhizophora apiculata leaf extract against Edwardsiella tarda bacteria Ronal Kurniawan
Jurnal Natur Indonesia Vol. 19 No. 1 (2021): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.19.1.13-17

Abstract

Aquaculture production is vulnerable to adverse impacts to disease and environmental conditions. One of the pathogenic bacteria that infection cultured is Edwardsiella tarda. The aim of this study was to find out the sensitivity of R.apiculata leaf extract in inhibiting the growth of E.tarda. The research method used is the experimental method, with the Kirby-Bauer disc method. The doses used were 100% (10000 ppm), 90% (9000 ppm), 80% (8000 ppm), 70% (7000 ppm), 60% (6000 ppm), 50% (5000 ppm), 40% (4000 ppm), 30% (3000 ppm), 20% (2000 ppm), 10% (1000 ppm) and control (Oxytetracycline), blank discs used were 6 mm in size. The results showed that the ethanolic extract of the leaves of R. apiculata at a dose of 1000-10000 ppm gave various inhibitory diameters ranging from 6.27-9.87 mm and presenting inhibition zone diameters in the medium category
The Addition of Guava Leaves in Feed to the Blood Glucose of Carp reared in Brackish Water and Infected with Aeromonas hydrophila Irwan Effendi; Windarti Windarti; Heri Masjudi; Muhammad Rizal Razman; Ahmed Al-Harbi; Nisfi Maulidia Nasution; Tomi Syahputra; Ronal Kurniawan
Jurnal Natur Indonesia Vol. 21 No. 2 (2023): October
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.21.2.156-161

Abstract

Guava leaves can be utilized as feed additives and medicine for aquaculture. Limiting factors in aquaculture, including stocking density, and salinity can cause stress in fish, so they are easily infected with disease. This study was conducted to determine the effect of guava on the blood glucose of carp reared in brackish water media. This research was conducted from August to September 2023 at the Marine Biotechnology Laboratory, Faculty of Fisheries and Marine, Universitas Riau. The method used in this study is an experimental method using a completely randomized design with 5 (five) treatments and 3 (three) replicates, the treatments are T0 (control), T1 (1.0g), T2 (1.5g), T3 (2 g), and T4 (2.5g). Carp measuring 5.00 ± 1.00 cm with a weight of 4.00 ± 0.50 g were obtained from farmers in the Koto Panjang hydroelectric reservoir, Sumatra, Indonesia. Fish rearing was carried out for 60 days. The results showed that the provision of guava leaves in the feed affected the blood glucose levels of carp. The dose of 1.5 g/100g feed (T2) was the optimal dose in increasing the blood glucose of carp reared in brackish water media and infected with A. hydrophila bacteria
Growth, Mortality, Exploitation Rate, and Recruitment of Blood Clam (Anadara granosa) in the Rangsang Barat Waters, Riau Province Efriyeldi Efriyeldi; Ronal Kurniawan
Jurnal Natur Indonesia Vol. 21 No. 1 (2023): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.21.1.7-14

Abstract

Growth, mortality, exploitation rate, and recruitment of Anadara granosa cockle in Rangsang Barat coastal waters were studied from July to October 2019. The objective of this research was to study the growth, mortality, exploitation rate, and recruitment of A.granosa cockle. Sampling was done monthly at three main stations. The cockle collected from plot 1 x 1 m2 on quadrat transect. The result showed that the asymptotic length (L∞) of A.granosa was 30.45 mm, the annual growth coefficient (K) was 0.83 per year, the total mortality (Z) was 4.46 per year, natural mortalities (M) were 1.60 per year, and fishing mortalities (F) was 2.86 per year. The rate of exploitation (E = 0.64) of blood clams in Rangsang Barat waters has begun to threaten sustainability. The recruitment occurred every month, the peaks occurred in July (13.83 %), and August (13.08 %).
Utilization of Sago Liquid Waste Organic Fertilizer as a Culture Medium for Chlorella sp. Irwan Effendi; Mega Novia Putri; Ulfi Laili Astika; Ronal Kurniawan
Jurnal Natur Indonesia Vol. 21 No. 1 (2023): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.21.1.55-62

Abstract

Chlorella sp. is a microalga that can grow and develop in wastewater media, including sago liquid waste that is not utilized and becomes a pollution material. This study aims to determine the utilization of sago liquid waste organic fertilizer on the growth of Chlorella sp. This research was conducted in February 2023 at the Marine Biotechnology Laboratory, Faculty of Fisheries and Marine Sciences, Riau University. The method used was the experimental method, by applying a complete randomized design (CRD) with four treatments and three replicates. The treatments were as follows: T0 (control), T1 (150 ml sago liquid waste), T2 (200 ml), and T3 (250 ml). The initial density of Chlorella sp. was 250 x103 cells/ml, and cell abundance observations were made for 14 days. The parameters observed were cell abundance, specific growth rate, and water quality (temperature and pH). The results showed that the utilization of sago liquid waste affected the abundance of Chlorella sp. 150 ml concentration and gave the best results on cell abundance of 233.33 x103 cells/ml with the peak population occurring on day 9. Water quality during the study was still in the normal range and can be tolerated for the growth of Chlorella sp, namely temperature ranging from 26-31o C and pH 6.2-8.0.
Inhibition Zone Test of Chaetomorpha sp. Extract Against Aeromonas hydrophilla and Vibrio sp. Bacteria Sri Wahyuni; Mohamad Gazali; Ronal Kurniawan
Jurnal Natur Indonesia Vol. 20 No. 2 (2022): October
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.20.2.50-54

Abstract

Chaetomorpha sp. is a green algae that has anti-bacterial compounds and at certain times found very much. The purpose of this study was to see the inhibition zone produced from the ethanol extract of Chaetomorpha sp. against A. hydrophilla and Vibrio sp. This research was done in March 2022. Extraction of Chaetomorpha sp., antibacterial effectiveness testing was done at the Biotechnology Laboratory, Faculty of Fisheries and Marine, Riau University. The method used is descriptive method. Sensitivity test was performed using the Kirby Bauer disc method. To reduce the error rate, it was repeated three times. The doses of macroalgae extract used were, doses of 100% (10,000 ppm), 90% (9,000 ppm), 80% (8,000 ppm), 70% (7000 ppm), 60% (6000 ppm), 50% (5000 ppm), 40% (4000 ppm), 30% (3000 ppm), 20% (2000 ppm), 10% (1000 ppm) and control (Oxytetracycline). Based on the observations, it was found that the use of ethanol extract of Chaetomorpha sp. The result is able to inhibit the growth of bacteria A. hydrophilla and Vibrio sp. at the highest dose (10000 ppm) in an range of 13.5 -14.5 mm which was classified as strong category, the lowest dose was at 5000 ppm as moderate category.
Inhibition Test of Hot Water Extract of Rhizophora apiculata Leaves Against Edwardsiella tarda Bacteria in-Vitro Ronal Kurniawan; Sri Wahyuni; Fitrian Armando
Jurnal Natur Indonesia Vol. 20 No. 1 (2022): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.20.1.30-34

Abstract

One of the plants that have the potential as antibacterial ingredients is Rhizophora apiculata leaf because it contains secondary metabolites, such as tannins, saponins, flavonoids, steroids, and terpenoids. This study aims to find out the inhibition of hot water extract of R. apiculata leaves in inhibiting the growth of Edwardsiella tarda. The research method used is an experimental method which includes sample preparation, extraction, antibacterial activity test using the Kirby-Bauer disc method and applying a Completely Randomized Design (CRD). The doses used were 100% (10000 ppm), 90% (9000 ppm), 80% (8000 ppm), 70% (7000 ppm), 60% (6000 ppm), 50% (5000 ppm), 40% (4000 ppm), 30% (3000 ppm), 20% (2000 ppm), 10% (1000 ppm) and control (Oxytetracycline). The results showed that the hot water extract of R. apiculata leaves at a dose of 1000-10000 ppm gave various inhibitory diameters ranging from 6.25-10.08 mm and the clear zone formed was classified as medium
POTENTIAL OF VARIOUS INDONESIAN MEDICINAL PLANTS TO INHIBIT THE GROWTH OF Aeromonas hydrophila BACTERIA Kurniawan, Ronal; Windarti, Windarti; Effendi, Irwan; Putri, Mega Novia; Syahputra, Tomi; Gusriansyah, Dimas; Riswan, M
Asian Journal of Aquatic Sciences Vol. 7 No. 2 (2024): August
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.7.2.299-304

Abstract

Aeromonas hydrophila is a gram-negative bacterium that can infect fish and is zoonotic to humans. Efforts to prevent this bacterial infection can be made using herbal plants due to the content of secondary metabolite compounds. This study aims to explore plants that have the potential as antibacterials, especially A. hydrophila bacteria. This research was conducted in February 2024 at the Marine Biotechnology Laboratory, Faculty of Fisheries and Marine Sciences, Universitas Riau. The experimental method used included the stages of preparation of plant leaf samples and antibacterial tests using Kirby-Bauer discs. The plants used were the leaves of senduduk (Melastoma malabathricum), kantong semar (Nepenthes sp), lime (Citrus aurantifolia), belimbing bukit (Acetosella barreligeri), sirsak (Annona muricata), mucuna (Mucuna bracteata), Israel grass (Asystasia gangetica), and miana (Coleus scutellariodes). The results showed that the leaves of kantong semar, senduduk, and belimbing bukit could inhibit bacterial growth in a strong category. In contrast, the leaves of sirsak, mucuna, Israel grass, miana, and lime were classified as moderately inhibiting the growth of A. hydrophila bacteria. In conclusion, these herbal plants can potentially prevent A. hydrophila bacterial infection
EVALUATION OF WATER QUALITY AND GROWTH PERFORMANCE OF RED TILAPIA (Oreochromis sp) IN CULTURE USING BIOFLOCK TECHNOLOGY Putra, Iskandar; Rusliadi, Rusliadi; Masjudi, Heri; Alfinda, Rudi; Asiddiqqi, Hasby; Syahroni, Desty; Kurniawan, Ronal; Firmansyah, Rodhi; Yulindra, Ade
Asian Journal of Aquatic Sciences Vol. 7 No. 2 (2024): August
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.7.2.305-311

Abstract

Water quality plays an important role in maintaining and increasing fish production. Tilapia (Oreochromis niloticus) is one of Indonesia's freshwater fish that has economic value. To increase production, tilapia cultivation is carried out intensively, characterized by high stocking density and provision of protein-rich feed. Good water quality control and feeding are key to the success of this intensive culture. Biofloc technology is one of the appropriate technologies for intensive tilapia rearing since fish can live at high densities and have a wide environmental tolerance. Biofloc technology is an alternative solution to problems in overcoming cultivation waste such as ammonia and nitrite. Based on the above, this study was conducted to determine the effectiveness of the biofloc system in converting ammonia compounds, improving water quality in the aquaculture environment, and understanding the relationship between water quality and tilapia growth. The research was conducted for 60 days to measure several water quality parameters, namely temperature, dissolved oxygen, acidity (pH), and ammonia, and to observe the growth parameters of tilapia. The treatment applied in this study was the addition of probiotics in feed with four treatments and three replications. The results showed that the biofloc system's water quality is tolerant to tilapia growth. The best growth results in treatment A with each value of absolute weight 14.70±0.10d, absolute length 6.70 ± 0.10d, specific growth rate 3.87±0.01c, and survival rate 100±0.00b
Co-Authors Ahmed Al-Harbi Al Zikri, Muhammad Abdillah Alfiati Silfi Alfinda, Rudi Ali Sarong Angraika Dirta Apriliani, Elisa Armando, Fitrian Asiddiqqi, Hasby Asmika Harnalin Simarmata Asri Mursawal Atthoriq, Fawwaz AZUZ, FAIDAH Davinci, Leonardo Deni Efizon Dessy Yoswaty Diana, Fitri Dimas Gusriansyah Eddiwan Kamaruddin Efawani, Efawani Efriyani, Intan Efriyeldi, Efriyeldi Eka Lisdayanti Fakhrurozi, Fakhrurozi Fikri, Algi Fari Firmansyah, Rodhi Fitri Kurniati, Fitri Fitrian Armando Fitrian Armando Gusriansyah, Dimas Hayatun Nufus Hayatun Nufus Hendri Kurniawan, Hendri Hendriyantoni, Agus Henni Syawal Henny Syawal Henny Syawal Husnul Yaqin Harahap Husnul Yaqin Harahap, Husnul Yaqin Iesje Lukistyowati Ika Kusumawati Irwan Effendi Irwan Irwan Irwan Limbong Karnila, Rahman Karnila Karsih, Okta Rizal M Irsyad Nur, M Irsyad M Rizal Mahdiyah, Evfi Mai Suriani Marbun, Serliana Hotmariska Mardhatila, Anisa Masjudi, Heri Mega Novia Putri Mega Novia Putri Meliana, Annisa Mohamad Gazali Mohamad Gazali Muhammad Natsir Kholis Muhammad Rizal Razman Munandar Munandar Mursawal, Asri Nabila Afifah Azuga Nasita, Abian Surya Nisfi Maulidia Nasution Nur Ikhlas Syuhada Nur Ikhlas Syuhada, Nur Ikhlas NURLAELA NURLAELA Nursyirwani, Nursyirwani Nurul Hidayat Okta Rizal Karsih Okta Rizal Karsih Oktavia Oktavia Priowirjanto, Gatot Hari Pulungan, Amar Doli Putri, Mega N. Putri, Mega Novia Putri, Nadila Yusmi Putri, Rianti Rahmiza, Nurul Ramadhan, Azhari Ramanda, Reski Riki Saputra, Riki Riswan, M Rita Rostika Rizka Aprisanti Rizki Lestari Rizvi, Fharisa Nabila Rizvi, Fharisa Putri Rulyanti, Mairisa Rusliadi Rusliadi Sahputri, Herliza Khairani Samudra, Arung Saputri, Jumaida Sari, Mira Rahmita Saryono Saryono Shofian Nanda Adiprayoga Shorea Khaswarina Sri Endang Kornita Sri Wahyuni Sri Wahyuni Sri Wahyuni Sri Wahyuni Sri Wahyuni Sri Wahyuni Syahputra, Tomi Syahril Nedi Syahroni, Desty Syam, Anandasya Suci Nabila Tirta Anugerah Tomi Syahputra Ulfi Laili Astika Ulfi Laili Astika Usiani, Lulu Usman Muhammad Tang Windarti Windarti Windarti Windarti Yuharmen - Yulindra, Ade Zidni, Irfan