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All Journal Media Penelitian dan Pengembangan Kesehatan Jurnal Kesehatan Masyarakat Folia Medica Indonesiana Jurnal Biosains Pascasarjana Indonesian Journal of Tropical and Infectious Disease The Indonesian Biomedical Journal Biomedical Journal of Indonesia Jurnal Teknologi Laboratorium Jurnal SainHealth Journal Of Widya Medika Junior Medical Laboratory Analysis and Sciences Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Indian Journal of Forensic Medicine & Toxicology Narra J Jurnal Kedokteran Hewan Jurnal Widya Medika
Jusak Nugraha
Clinical Pathology Department, Dr. Soetomo Hospital Surabaya Institute Of Tropical Disease, Universitas Airlangga Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Veterinary Other

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Journal : The Indonesian Biomedical Journal

 1 
The Effect of Thymoquinone Administration on Local Immunoglobulin-G Levels of Rattus norvegicus Strain Wistar Sciatic Nerve Crush Injury Model Valentinus Besin; Abdul Hafid Bajamal; Mohammad Hasan Machfoed; Jusak Nugraha; Budi Utomo; Paulus Budiono Notopuro
The Indonesian Biomedical Journal Vol 14, No 1 (2022)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v14i1.1772

Abstract

BACKGROUND: Immunoglobulin-G (IgG) is a product of the initial response to secondary immune response, which accumulates in distal segment of the nerve after crush injury. Thymoquinone modulates the adaptive immune response. Effect of thymoquinone administration on local IgG levels of Rattus norvegicus Wistar rats sciatic nerve crush injury model has not been elucidated.METHODS: This was an experimental study, with 63 Rattus norvegicus Wistar rats that divided into 9 groups. Three groups were given placebo, 3 groups were given 100 mg/kg/day thymoquinone, and 3 groups were given 250 mg/kg/day thymoquinone. The rats were terminated based on the assigned group at 5x24, 6x24, and 7x24 hours and then the IgG levels were measured using sandwich enzyme-linked immunosorbent assay (ELISA).RESULTS: There was a significant difference in IgG levels after administration of 100 and 250 mg/kg/day thymoquinone at 5x24 hours and 7x24 hours post-injury compared to the rats that were given no treatment. A significant difference of IgG levels was also found after administration of 100 mg/kg/day thymoquinone group at 6x24 hours post-injury. Critical point of decreasing local IgG of all groups happened at 6x24 hours after injury, however, there was no significant difference in the median levels of thymoquinone at doses of 100 mg/kg and 250 mg/kg.CONCLUSION: Local IgG levels in distal segment of the sciatic nerve crush injury is lower in rats that were given 100 mg/kg thymoquinone treatment compared to the rats that receive no thymoquinone treatment since 5x24 hours after injury. Thymoquione administration should be given immediately after the crush injury until before 6x24 hours post-injury to decrease antibodies in degeneration process.KEYWORDS: thymoquinone, immunoglobulin-G, crush injury, sciatic nerve
2-(3-(chloromethyl)benzoyloxy)benzoic Acid Increases CD4+ Regulatory T-Cell Population and FoxP3 Expression in Lipopolysaccharide-induced Mice Yudy Tjahjono; Caroline Caroline; Jusak Nugraha; Kuncoro Foe; Srikanth Karnati; Süleyman Ergün; Nico Jafet; Oryza Chrisantia; I Made Andika Bara Kusuma; Hendy Wijaya; Wuryanto Hadinugroho; Dwi Aris Agung Nugrahaningsih; Dwi Liliek Kusindarta; Yufita Ratnasari Wilianto; Senny Yesery Esar; Hevi Wihadmadyatami
The Indonesian Biomedical Journal Vol 15, No 4 (2023)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v15i4.2429

Abstract

BACKGROUND: Lipopolysaccharide (LPS) has been reported to increase CD4+ regulatory T-cell (CD4+ Treg) populations. Acetylsalicylic acid (ASA) has been reported to have immunomodulatory activity, but it may induce chronic gastric ulceration. Another salicylic acid-bearing compound, 2-(3-(chloromethyl)benzoyloxy)benzoic acid (3-CH2Cl), has been reported to have less gastric mucosal damage. However, the effect of 3-CH2Cl on CD4+ Tregs in LPS-induced mice is still unknown. Therefore, the present study was conducted to investigate the immunomodulatory effect of 3-CH2Cl on CD4+ T-cell and CD4+ Treg populations as well as FoxP3 expression in LPS-induced mice.METHODS: Synthesis of 3-CH2Cl was performed by mixing salicylic acid and chloromethylbenzoylchloride with the catalyzation of pyridine, acetone and heat. The 3-CH2Cl tablets were prepared using direct compression method. After intraperitoneal injection of 1 mg/kg BW LPS to mice, 60 mg/kg BW ASA or 60 mg/kg BW 3-CH2Cl was given orally for 3 days. The splenocyte was obtained through splenectomy and collagenase digestion. The population of CD4+ T-cells and CD4+ Tregs, as well as the splenic FoxP3 expression were determined using flow cytometry technique.RESULTS: CD4+ T-cell populations in mice treated with LPS and 3-CH2Cl or ASA were lower than those treated with LPS merely. Meanwhile, CD4+ Treg populations and FoxP3 expression levels in mice treated with LPS and 3-CH2Cl or ASA were higher than those treated with LPS merely.CONCLUSION: Since 3-CH2Cl could decrease CD4+ T-cell population and increase CD4+ Treg population mediated by the increase of FoxP3 expression in LPS-induced inflammation, it may act as a potential therapeutic drug to reduce inflammatory conditions.KEYWORDS: 2-(3-(chloromethyl)benzoyloxy)benzoic acid, acetylsalicylic acid, CD4, T-regulatory cells, FoxP3, LPS
Co-Authors Abdul Hafid B Agung Dwi Wahyu W Agung Dwi Wahyu Widodo Agustiningrum, Indah Akhmad Setyo Rahman aminuddin, mohammad Anak Agung Istri Sri Wiadnyani Anita Budiarti Anna Roosdiana Anton Yuntarso Aryati Aryati Aryati Aryati Ashon Sa'adi Ashon Sa’adi Ashon Sa’adi Awalia Awalia Ayu Imamatun Nisa Bambang Purwanto Bastian Bastian Bastian Bastian Beatrix, Stephanie Besin, Valentinus Betty Agustina Betty Agustina Tambunan Buana, Dwi Candra Budi Prasetyo Budi Utomo Budiutari, Ni Nyoman Budy, Theresia Indah Callixte, Cyuzuzo Caroline Caroline Ch. Destri Wiwis Wijayanti Christina Destri Citra Indah Setyaningrum Cynthia Cynthia Cynthia Cyuzuzo Callixte Darmadi, Epriyanto T Dheasy Herawati Diyan Wahyu Kurniasari Djoko Agus Purwanto Dominicus Husada Dwi Aris Agung Nugrahaningsih Dwi Liliek Kusindarta Dwiyanti Puspitasari, Dwiyanti Eddy Mart Salim Eddy Mart Salim Eddy Mart Salim Eddy Mart Salim, Eddy Mart Edhi Rianto Elok Budi Retnani Elvan Dwi Widyadi Erfina Lim Erna R Tobing Esthy Poespitaningtyas Febtarini Rahmawati Ferdy R. Marpaung Ferdy Royland Marpaung Foe, Kuncoro Francisca Srioetami Tanoerahardjo Francisca Srioetami Tanoerahardjo Fransisca Srioetami Tanoerahardjo Fransisca Srioetami Tanoerahardjo Fransiska Fransiska Fransiska Sri Oetami Gede Arie Wijaya Handayani, Luh Putu Trys Monika Hans Kristian Nugraha, Hans Kristian Hantoro Gunawan Hari Basuki Notobroto Hari Setiono Hartono Kahar, Hartono Hendy Wijaya Herin Mawarti Heru Setiawan Hery Priyanto Hevi Wihadmadyatami Holland Lydia Marpaung Huda Shalahudin Darusman I Made Andika Bara Kusuma Idha Kusumawati Ilham Ilham Irvan Ipandi Januarti, Catur Ifda Joewono Soeroso John Wiwin Julious Julious julious, julious Kasiyati, Menik Kemas Ya'kub Rahadiyanto Kemas Ya'kub Rahadiyanto Kemas Ya’kub Rahadiyanto kurniati, nova Lisa Soegianto Lumempouw, Silvia Luminto, Dian Lutfi Dewanda Nugroho M. Aminuddin M. Aminuddin M. Irsan Saleh Maya E. Roring Meiti Muljanti, Meiti Mohammad Aminuddin Mohammad Hasan Machfoed Muhammad Amin Muhammad Amminuddin Muhammad Hamdan Muhammad Irsan Saleh Muhammad Nazarudin Munawaroh Fitriah Myrza Elita Nico Jafet Ningrum, Emilna Mega Ninik Asmaningsih Soemyarso Notopuro, Paulus Budiono Nova Kurniati Nova Kurniati Nova Kurniati Noviana, Rachmitasari Nugraha, Hans K Nur Hayati Nur Lailatul Fadhilah Oetami, Fransisca Sri Oryza Chrisantia Patria Dewi Pande Pratama, Dyah Ayu Oktaviani A Prihantika S., Sabrina Pudji Lestari Purwanta, Marijam Purwoko, Agus Puspitasari, Yessy Putri, Indah Aprianti Rahadiyanto, Kemas Ya'Kub Rahajuningsih Dharma Rahma Indah Pratiwi Rendy, Achmad Nur Rifa’I, Muh Husni Risky V. Prasetyo Roedi Irawan Rusli, Musofa Ryzky Widi Atmaja S. Soedarsono S.Pd. M Kes I Ketut Sudiana . Sabri Prihantika Sabrina Prihantika Saleh, M Irsan Saputra, Angky Saputri, Agatha Efrad Senny Yesery Esar Setiawan, Lidwina Tri Kristanti Shahzad Shoukat Sheila Clarissa Shoukat, Hamad Shoukat, Shahzad SILMI MARIYA Silvia F. Lumempouw Sinansari, Restry Siti Nur Husnul Yusmiati Siti Rahmawati Soedarsono Soedarsono Soedarsono Soedarsono Soelistijo, Soebagijo Adi SP Edijanto Srikanth Karnati Süleyman Ergün Susi Oktaviani Tanoehardjo, Francisca Srioetami Tanoehardjo, Francisca Srioetami Tanoerahardjo, Francisca Srioetami Tedja, I G.A. Wiradari Theresia Indah Budhy Sulisetyawati Triagung Ruddy Tuyishimire Irene Utariani, Arie UUS SAEPULOH Wahyu Dewi Tamayanti, Wahyu Dewi Wahyu Setiani Wibowo Wahyu Setiani Wibowo Winthoko, Eka Nora Vitaloka Aprilia Putri Wulandari, Dian Novita Wuryanto Hadinugroho Yetti Hernaningsih Yoes Prijatna Dachlan Yudy Tjahjono Yufita Ratnasari Wilianto Yulia Nadar Indrasari Zen Hafy