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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Journal of Indonesian Tourism and Development Studies Biotropika Jurnal Pembangunan dan Alam Lestari AGRIVITA, Journal of Agricultural Science Journal of Tropical Biodiversity and Biotechnology TREUBIA JSMARTech : Journal of Smart Bioprospecting and Technology Proceeding Biology Education Conference RISTEC : Research in Information Systems and Technology Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Nia Kurniawan
Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences

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Computational Insight on Bioactive Compound Potential Activity of Kenitu (Chrysophyllum cainito L.) Leaves as PPAR-y Antagonist for Obesity Rosyada, Nabila Nur; Andyni, Regina Shania; Alvionita, Cicin Vinolia; Narwasthu, Sekararum; Adawiyah, Robiatul; Hermanto, Feri Eko; Kurniawan, Nia; Fatchiyah, Fatchiyah
Biotropika: Journal of Tropical Biology Vol. 12 No. 2 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2024.012.02.03

Abstract

Studies reported that kenitu (Chrysophyllum cainito) has the potential as an anti-obesity agent. However, the mechanism underlying that activity remains unknown. On the other hand, targeting PPAR-y has played a beneficial role in regulating obesity. Therefore, this study will explain the anti-obesity potential of natural compounds from C. cainito in modulating obesity through inhibition of PPAR-y. Molecular docking was employed to identify the possibility of several compounds binding with the PPAR-y. Moreover, the stability of the interaction between PPAR-y and each compound was also evaluated using molecular dynamics analysis. The result showed that beta-sitosterol, lupeol, myricitrin, quercitrin, rutin, vitamin A, epicatechin, and chlorogenic acid are worthy of potential as PPAR-y antagonists. In advance, molecular dynamics simulations revealed that chlorogenic acid, quercitrin, and epicatechin were the most stable compounds to interact as an inhibitor of PPAR-y. In conclusion, compounds from star apple have some potential to improve the health status of patients’ obesity through PPAR-y antagonist activity.
Assessing the Genotoxicity Effect of a Commercial Chlorpyrifos Formulation in Fejervarya limnocharis Tadpoles (Anura: Dicroglossidae) Under Acute and Chronic Exposure Ramadani, Shima; Marhendra, Agung Pramana Warih; Kurniawan, Nia
The Journal of Experimental Life Science Vol. 12 No. 1 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.01.02

Abstract

The potential for genotoxicity of pesticides is currently one of the world's concerns. Chlorpyrifos is the organophosphate active ingredient with the largest sales, but the potential for genotoxicity in amphibians is still not widely known. The purpose of this study was to assess the genotoxicity effect of a commercial chlorpyrifos formulation Dursban 200EC in F. limnocharis tadpole erythrocyte (Anura: Dicroglossidae) under acute and chronic exposure using by micronucleus assay. Acute and chronic toxicity tests consisted of negative control, positive control, and 0.4, 0.8, and 1 µg.L-1 of chlorpyrifos with three replications. A toxicity test was carried out on ten tadpoles (Gosner 25) from artificial reproductions in each treatment. The results showed that the formulation of Dursban 200EC in low concentrations (0.4 µg l-1) had the potential to induce DNA damage in erythrocytes of F. limnocharis tadpoles, and there was a positive correlation between chlorpyrifos concentrations and an increase in the frequency of MN. Erythrocytes exposed to chlorpyrifos in both acute and chronic treatment had significantly different MN frequencies between negative and positive controls, 0.4, 0.8, and 1 µg.L-1 (p<0.01). Meanwhile, positive controls were not significantly different from 1 µg.L-1 ( p>0.05). However, the increase in the frequency of MN in chronic treatment was almost twice as high.
Morphological Variation of Asian Small Lizards genus Tytthoscincus Linkem, Diesmos & Brown (Squamata : Scincidae) in Indonesia Firmansyah, Richo; Riyanto, Awal; Kurniawan, Nia
The Journal of Experimental Life Science Vol. 13 No. 2 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.02.05

Abstract

Morphological variations in species with a relatively broad distribution, including the genus Tytthosincus, were likely a result of island isolation. This research was conducted to map the morphological variations of the genus Tytthoscincus in Indonesia. A total of 32 specimens of the Tytthoscincus scientific collection in the Zoologicum Bogoriense Museum (MZB) - National Research and Innovation Agency (BRIN) and identified as T. temmincki, T. textus, and T. parvus have been examined and data collection which includes six morphometric characters and 12 meristic characters. Morphometric data were analyzed in univariate and multivariate. Univariate analysis was carried out by the Kruskal-Wallis test, while multivariate analysis was done with the Principal Component Analysis (PCA) test. The results showed morphology in T. temmincki and T. textus, namely the number of loreal scales and tympanum diameter. Both T. temmincki and T. textus consist of two groups, but the morphological variation in T. temmincki and T. textus in this study has yet to provide certainty as a different species. However, one was T. textus Type 2, isolated from other species on Jampea Island, Sulawesi. Further research was needed to clarify the taxonomic status of these variations by increasing the number of adequate samples supported by the molecular analysis approach. Keywords: meristic, morphometric, species, Tytthoscincus, variation.
Corrigendum: Morphological Variation of Asian Small Lizards Genus Tytthoscincus Linkem, Diesmos & Brown (Squamata : Scincidae) in Indonesia Firmansyah, Richo; Riyanto, Awal; Kurniawan, Nia
The Journal of Experimental Life Science Vol. 13 No. 2 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.02.10

Abstract

CORRIGENDUM.
Phylogenetic Inference on Limnonectes kuhlii Complex in Java and Sumatra Reveals Significant Novel Diversity Fahmi, Muhamad; Kadafi, Ahmad Muammar; Priambodo, Bagus; Fauzi, Muhammad Alif; Hamidy, Amir; Firdaus, Anggun Sausan; Smith, Eric Nelson; Kurniawan, Nia
The Journal of Experimental Life Science Vol. 13 No. 3 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.03.02

Abstract

The fanged frog, Limnonectes kuhlii, exemplifies the complexity of cryptic species. Though originally described from Java, subsequent studies indicate that L. kuhlii encompasses multiple lineages, suggesting the presence of several undescribed species. Suspecting the existence of multiple undescribed species within the L. kuhlii complex in Sumatra, we collected 17 specimens from Java and Sumatra. We extracted the 12S, tRNAval, and 16S mitochondrial DNA from these specimens for phylogenetic analysis using Maximum Likelihood and Bayesian Inference methods and for estimating time divergence. Our findings uncovered two novel diversity within the L. kuhlii complex both in Sumatra and Java. Furthermore, our estimation of time divergence suggests that the diversification of L. kuhlii in Java and Sumatra was influenced by geological and climatic events, including landmass emergence and sea-level fluctuations. In conclusion, our research provides critical insights into the evolutionary complexity and diversity of the L. kuhlii complex in Sumatra and Java. While our molecular evidence suggests novel diversity, further comprehensive morphological studies are imperative to confirm and describe these potential new species. Furthermore, additional sampling across its range and deeper investigations integrating both molecular and morphological data are crucial. Keywords: fanged frogs, Limnonectes kuhlii, Limnonectes sisikdagu, phylogenetics.
Effectiveness of Extract Supplements (Apium graveolens L.) to Estrogen Receptors Expression and Oocyte Diameter at Nucleolar Chromatin and Perinucleolar Stages in Female of Nile tilapia (Oreochromis niloticus L.) Rikardo, Novy Kurnia; Marhendra, Agung Pramana Warih; Kurniawan, Nia; Riawan, Wibi; Rahayu, Sri
The Journal of Experimental Life Science Vol. 13 No. 3 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.03.03

Abstract

The study of the use of celery (Apium graveolens L.) in the health sector has been widely discussed. The analysis of the use of celery in the process of oocyte development still needs to be addressed. This study was conducted to see the effect of celery extract on the expression of estrogen receptors on the gonads and oocyte diameter in tilapia for the first time. Fish are divided into four groups: P0 = Control treatment, feed without mixture; P1 = Treatment with celery extract concentration of 2,000 mg.kg-1 of feed; P2 = Treatment with celery extract concentration of 4,000 mg.kg-1 of feed; P3 = Treatment with celery extract concentration of 6,000 mg.kg-1 of feed. The results showed celery extracts expressed estrogen receptors on the gonads. The expressed estrogen receptor is the estrogen receptor β, whereas the estrogen receptor α is not detected in the gonad. Group statistical tests ANOVA showed no significant results in the development of oocytes. On the other hand, celery extract also impacted the development of oocytes at the nucleolar stage of chromatin, with a markedly greater oocyte diameter. Meanwhile, in the perinucleolar stage, celery extract reduces the oocyte diameter. Keywords: Apium graveolens L, ERα, ERβ, nucleolar chromatin, perinucleolar.
Could Road Structures Impact the Avian Community? A Study Case from the South Coast Remained Forest in Malang Region, East Java Province, Indonesia: Impact of Road Structures on Avian Community Nurrofik, Agus; Hakim, Luchman; Septiadi, Luhur; Kurniawan, Nia
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.10

Abstract

The remaining tropical forest on the south coast of the Malang region is one of the secluded areas that served as an important habitat for its biodiversity, particularly for avians. Nevertheless, the presence of the road structure that crosses over the forest might impact avian communities, which needs to be investigated. The avian survey was conducted to investigate the diversity, community profiles, abundance, and feeding guild based on two different ecosystem patches (on the roads versus outside the road structures) during January–April 2022. The audiovisual encounter methods were performed during the surveys on a total of ten sampling points. Avian species were identified using field guides where the conservation status was based on the national regulation (P106 KLHK), CITES, and IUCN Redlist. The comparison through the avian community based on species richness was analyzed using Venn Diagrams and predicted using rarefaction and interpolation curves on INEXT packages. Additionally, the comparison of sizes of avian abundance was investigated using the α-diversity parameter index, and the feeding guild was determined by five diet guilds. In total, 2536 individuals from 67 species, 34 families, and 13 orders were found during the survey. The avian communities were richer outside the road structure, but their abundance qualitatively reveals that both different ecosystem patches showed relatively good condition. Overall, road structures generate both positive (unique habitat patches) and negative (physical barriers that could disrupt natural ecological processes) impacts on avian diversity. In light of these, sustainable management and conservation action plans were urgently needed to prevent the negative effects on avian communities posed by the road structure.
Molecular characterization of genus Tor from Indonesia based on 16S rRNA Rahayu, Dwi Anggorowati; Nugroho, Endik Deni; Azrianingsih, Rodiyati; Kurniawan, Nia
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 7 No. 01 (2022): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v7i01.1880

Abstract

Genus Tor is Indonesia's endangered local fish however, the reports on this species from South East Asia is currently limited. This study conducted to characterize molecular genetics and phylogenetic relationship of Tor fish from East Java, West Kalimantan, Padang and North Sumatra from BPBAT Bogor collection using a partial sequence of mtDNA 16S rRNA. A total of ten samples of Genus Tor were collected, then identified based on morphological characters, and the identification was confirmed using molecular data. PCR amplicons at 542 bp in length. The construction of phylogenetic topology was made based on ML and NJ method using Kimura-2 parameter model. Based on the phylogenetic topology showed that Tor fish from Pasuruan are closely related to Tor duoronensis from Padang with a bootstrap value of 66%, while Tor duoronensis fish from North Sumatra and Tor tambra from West Kalimantan are in separated clusters. This finding also contributes to the differentiate Genus Tor from Indonesia based on polymorphic sites
The Use of Personal Inarisk Media in Improving the Concept of Disaster Information Literacy in social Studies Learning Kurniawan, Nia; Mulyana, Eldi; Nopharipaldi Rohman, Slamet; Widyanti, triani; Tetep; Supriyatna, Asep; Nugraha, Yopi
RISTEC : Research in Information Systems and Technology Vol. 1 No. 1 (2020): JURNAL RISTEC : Research in Information Systems and Technology
Publisher : RISTEC : Research in Information Systems and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The purpose of this study is to determine the improvement of the concept of disaster information litera cy using InaRisk Personal lea rning media in socia l studies learning. This research approa ch uses quantitative with quasi-experimenta l method with nonquivqlent control group design. The population of this resea rch is the seventh grade students of SMP Negeri 1 Ta rogong Ka ler. The sample wa s taken by purposive sampling technique with cla ss VII A as the experimental cla ss and class VII B as the control class. Based on the results of statistical ana lysis, the Asymp.sig.2-tailed value is 0.000 and a is 0.05, which means 0.000 <0.05, then Ho is rejected. From these data it can be concluded that the value of Asymp.sig. of 0.000 < 0.05, then there is a significant increase or lea rning that uses InaRisk Personal lea rning media is effective to be applied. Overa ll, the ability of the concept of disaster information litera cy affects the prepa redness of students in dealing with disasters in the surrounding environment. the ability of the concept of disaster information literacy in the experimental cla ss increased significantly in terms of seeking information, identifying information, evaluatinginformation, andusinginformation.
Insect as Biological Indicator from Protected to The Disturb Landscape in Central Java Indonesia Wicaksono, Karuniawan Puji; Suryanto, Agus; Nugroho, Agung; Nakagoshi, Nobukazu; Kurniawan, Nia
AGRIVITA Journal of Agricultural Science Vol 33, No 1 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i1.46

Abstract

In the biological science, invertebrate (especially insect) diversity is relatively well known. Yet, little study about their interaction with specific land use or specific system function. With the rapid changes of landscape, biodiversity is also changes in response to human impact; due to each organism have the specific interaction with certain environment. In this research, the assessment of insect order in the different landscape types was conducted using several method of trapping to understand the specific pattern of insect which are inhabited the landscape. The objectives of this research were monitored the Insect diversity, its ecological importance to agro-forestry ecosystem, and compare it with other forest type in this area. Another objective was determined the insect characteristic as the indicator of environmental quality on each land-use system (forest, agroforestry, plantation and monoculture). Monoculture agriculture has the largest number of Lepidoptera and Hemiptera order (herbivore insect dominated) while in agro-forest system has the largest number of Diptera and coleoptera order. Protected forest, plantation forest and agro-forestry showed the similar index number which shows the similar ecological services for the insect as their habitat. However, in the monoculture agriculture, there was an unbalance insect composition and high dominance.Keywords: landscape, biodiversity, environment indicator index
Co-Authors Abdullah Abdullah Achmad Dadang Burhanuddin Adityas Arifianto Agung Pramana W.M Agung Pramana W.M Agung Pramana Warih Marhendra Agung Pramana Warih Marhendra, Agung Pramana Warih Agung S. Kurnianto Agung Sih Kurnianto Agung Sih Kurnianto Agung Sih Kurnianto Agus Nurrofik Agus Suryanto Aguung Sih Kurnianto Ahmad Muammar Kadafi Alvionita, Cicin Vinolia Amin Setyo Leksono AMIR HAMIDY Amir Hamidy Andri Maulidi Andri Maulidi Andyni, Regina Shania Anggun Sausan Firdaus Aninnas, Afina Ardiansyah, Franky Ardiantoro, Ari Ardyah Ramadhina Irsanti Putri Arief Sugiharto Arifianto, Adityas Asmaul Khusnah Asmaul Khusnah Assiddiqy, Miftah Farid Azrianingzih, Rodiyati Azrianingzih, Rodliyati Badiul Ulum Badiul Ulum Bagus Priambodo Bagyo Yanuwiadi Bayu Hendra Prakosa Catur Retnaningdyah Darojat, Annisa Zakiyah Dinana, Ichda Arini Djong Hon Tjong Dwi Anggorowati Rahayu Eldi Mulyana, Eldi Elsa Mufti Endik Deni Nugroho Estri Laras Arumingtyas Fahmi, Muhamad Faldy Alifianto fatchiyah . Fauzi, Muhammad Alif Firdaus, Anggun Sausan Firmansyah, Richo Fitra Arya Dwi Nugraha Fitra Arya Dwi Nugraha Franky Ardiansyah Gigih Setia Wibawa Haryanti Haryanti Haryono Haryono Herlin Aprilia Kartini Herlin Aprilia Kartini Hermanto, Feri Eko Imti Yazil Wafa Karina hanum wardany Karina hanum wardany Karuniawan Puji Wicaksono Kholilah, Tsaniyah Nur Kurnianingsih, Nia Kurnianto, Aguung Sih Lekatompessy, Vanela Chatrin Lingga Nugraha Luchman Hakim Lutfita Fitriana Lutviyana Rahman Lutviyana Rahman Maharani, Tri Mahmudul Hasan, Mahmudul Maisuroh, Dalilatul Masayuki Sumida, Masayuki Muhammad Fathoni, Muhammad Mumpuni Mumpuni Narwasthu, Sekararum Nashi Widodo Nasirudin, Mohamad Nobukazu Nakagoshi Nopharipaldi Rohman, Slamet Nugraha, Yopi Nugroho, Agung Nurrofik, Agus Prabasari, Kartika Pramudya, Muhammad Alif Imam Putri Ramadhani, Putri Putri, Nenis Try Melani Putri, Siti Aqila Kharisma Qothrun Izza Qothrun Izza Ramadani, Shima Rijalullah, Muhammad Asyraf Rikardo, Novy Kurnia Riyanto, Awal Robiatul Adawiyah Rodiyati Azrianingsih Rosyada, Nabila Nur Rosyadah, Nuraini Rudi Candra Aditama Rudi Candra Aditama Septiadi, Luhur Shahroni, Abdul Mutholib Smith, Eric Nelson SRI RAHAYU Sugiharto, Arief Suharjono, Suharjono Suparno, Kelik Supriyatna, Asep Takeshi Igawa, Takeshi Tesri Maideliza Tetep Tri Maharani Triani Widyanti, Triani Vendy Adrinanda Vendy Adrinanda Wibi Riawan Widodo Widodo Widyananda, Muhammad Hermawan Zain, Dhiyaa Syahlaa Bianca Febrinnisa