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Journal : Life Science and Biotechnology

In Silico Primer Design for geographical detection of Apis florea using Cytochrome c oxidase subunit 1 (COX1) gene Shullia, Nurul Insani; Kuswati, Kuswati; Kurniawan, Aditya; Fiarani, Hajar Syifa
Life Science and Biotechnology Vol. 1 No. 1 (2023): May
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i1.40052

Abstract

The yellow dwarf honey bee, Apis florea are well distributed in South Asia to South East Asia. This species is expanded and introduce area from their original distribution. However, the distribution of this honey bee in Indonesia is unexplored. The cytochrome c oxidase subunit 1 (COX1) gene are success to detect original geographic of introduce A. florea found in Egypt. The A. florea specific primer of COX1 gene are needed to produce the molecular marker for geographical origin detection. Thus, this study aims to in silico design the COX1 gene primer of A. florea using Primer3 and Primer-BLAST. This study results the best candidate primer of Af6_COX1_F and Af6_COX1_R primers that start from 212 to 983 regarding the A. florea COX1 gene (NC_021401) and produce 772 bp of amplicon. The melting temperature of forward primer are 54. 63 and 55.58°C, GC content of 40 and 45%, GC clamp of 3 and 2 for forward and reverse primer, respectively. There are no secondary structure of those primer and 100% homolog with A. florea COX1 sequences, thus those primer is potentially as the geographical origin marker of A. florea.
Co-Authors A. P. A. Yekti Abdul Muhaimin Abdul Rohman ACHMAD FARAJALLAH Aditya Kurniawan Agus Susilo Agus Susilo Ahmad Furqon Ahmad Furqon Ajeng G. Nanda, Ajeng G. Ali mahfud Ali Mahfud Alifian Ibnu Ansori Andi Murlina Tasse Andra Widiastutik Andriawan Soni Hantoro Anggun Permata Sari Ardhina Firdausi Aris Sutomo Aryogi Aryogi Aryogi Aryogi Asri Nurul Huda Asri Nurul Huda Aulia Puspita A Yekti Aulia Puspita Anugra Yekti Awang Tri Satria B. Susilo Bayu Dewantoro Putro Soewandi Bella, Frimita D. Muhammad Dandy Prasetyo Dewi , Meinasari kurnia Dian Kumalasari, Elis Dicky Pamungkas Doni Herviyanto Dwi N. Ahsani, Dwi N. Dwi Retna Prihati, Dwi Retna Dwi Susan Setiyani Dwijayanti, Indah Dyah Perwitasari Eko Nugroho Elvira, Ajeng Erin Ayu Octaviani Erlangga Arfiyan Nur Erlia Narulita Ety Sari Handayani Fatimah, Jesy Febrianti, Riska Ayu Ferdy Saputra Fiarani, Hajar Syifa Gani, Tifany Sonia Gatot Ciptadi Hamidah Ikhwan, Salma Nur Hary Nugroho Henik Istikhomah, Henik Hermansyah, Alfandy Herviyanto, Doni Herviyanto, Dony Husnassabil, Ahmad Hadin Ikhsanuddin Ikhsanuddin Ikhsanuddin, Ikhsanuddin Imam Mudakir Indrayani, Tsaniya Ahda Indriyani Indriyani J. A. E. Noor Joko Waluyo Kaiin, Ekayanti Mulyawati Khairul Rasyad Khairunnisa, Icha Kurniaesa, Titah Ulya Kusmartono Kusmartono L. A. Sunarto Latifah Hanum Lina Winarti M. Luthfi M. Nasich Marini . Marjuki Marjuki Mashudi Mashudi Mashudi Mashudi Maulid, Shintia Sukmawati Cahya Moch Nasich Moch Sasmito Djati Moch. Syamsul Arifin Zein, Moch. Syamsul Arifin Mohammad Wildan Habibi, Mohammad Wildan Muhamad Iqbal Zajulie Muhammad Irvan Ali Muhammad Zainul Hanif Mukhlas Agung Hidayat N. Isnaini Nareswara Hapsari Ndaru Pranowo Widiasmoro Nisa’us Sholikah noviyani, ernita prima Nurgiartiningsih, V. M. A. Nurjazuli Nurjazuli Nurmasitoh, Titis Nurrasyidah, Rahmi Nurul Huda, Asri Nurul Insani Shullia Nurul Isnaini Nurul Layla Oktriyani, Erni Onni Meirezaldi Paskah Partogi Agung Pratiwi, Herlina Priyo Sugeng Winarto Puguh Surjowardojo Pujiastuti Pujiastuti, Pujiastuti Purwanto, Didiek Putri Utami Putri Utami Putri, Aprillia Ika Putri, Rizqi Wulandari R Sa’diyah, Kusnul Rahmanti, Fishella Aprista Raihanny, Yasmin Ravenska Ravenska Rifai Mustofa Rini Dwi Wahyuni Rini Dwi Wahyuni Risky Amalia Rosary Rizki Prafitri Rizki Prafitri Rofidaa, Asta Rosalinda, Julitha D Rosalinda, Julitha Dwi Rusdiana, Rina Sa'diyah, Kusnul Rodhiyatul Safitri, Putri Fina A’rafiani Safitri, Sinda Mercy Saputra, Muhammad Rizky Sari, Agustina Siti Hodijah Sri Wahjuningsih Sucik Maylinda Sucik Maylinda Sugesti, Madiyan Surjowadojo, Puguh Sutomo, Aris Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Syahruddin Said T. Susilawati T. Susilawati Tri Eko Susilorini Tri Eko Susilorini tri wahyuni Trinil Susilawati Triwik Sri Mulati Veronica Margareta Ani Nurgiartiningsih Wicaksana, Zulfikar Loka Widi Nugroho Widiasmoro, Ndaru Pranowo Wike andre Septian Wiranto Wiranto Yadi Malda Yayuk Fatmawati, Yayuk Yulian, Ria Zainuddin Zainuddin Zainuddin, Zainuddin Zainuri Sabta Nugraha, Zainuri Sabta Zhafirrahman, Muhammad Zulfaini Shamad