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All Journal International Journal of Evaluation and Research in Education (IJERE) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Pendidikan Sains Biogenesis: Jurnal Ilmiah Biologi Biotropika Proceeding International Conference on Global Resource Conservation Jurnal Pendidikan Biologi Indonesia Biology, Medicine, & Natural Product Chemistry Mutiara Medika: Jurnal Kedokteran dan Kesehatan Bioeksperimen: Jurnal Penelitian Biologi Journal of Tropical Biodiversity and Biotechnology Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan Jurnal Pendidikan Biologi Sekolah Dasar: Kajian Teori dan Praktik Pendidikan BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Penelitian Pendidikan IPA (JPPIPA) BIOEDUKASI Biosfer: Jurnal Pendidikan Biologi Saintifik : Jurnal Matematika, Sains, dan Pembelajarannya Jurnal Jamu Indonesia Bio-Inoved : Jurnal Biologi-Inovasi Pendidikan JPPIPA (Jurnal Penelitian Pendidikan IPA) JPP (Jurnal Pendidikan dan Pembelajaran) Proceeding Biology Education Conference Prosiding Seminar Nasional Biologi, Teknologi dan Kependidikan (Biotik) Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Jurnal PRIMED:Primary Education Journal atau Jurnal Ke-SD An Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Jurnal Kedokteran Hewan Jurnal Ilmu Hayat Indonesian Journal of Jamu
Betty Lukiati
Universitas Negeri Malang
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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Potential of Single Garlic to Prevent Pro Inflammatory Macrophage and Inflammation in HFD Mice Putri Diyah Anggraini; Miftahul Mufinadiroh; Hendra Susanto; Betty Lukiati; Sri Rahayu Lestari
Journal of Tropical Life Science Vol. 12 No. 1 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.01.10

Abstract

A high-fat-enriched diet causes an increase in the level of oxidized LDL (Ox-LDL) in the blood. The presence of Ox-LDL will activate macrophages to secrete pro-inflammatory cytokines and lead to severe inflammation. Single bulb garlic has a potential anti-inflammatory effect due to of high-fat diet. This research aimed to investigate the effect of single bulb garlic extract (SBGE) on the pro-inflammatory cytokines TNF-α (CD11b+TNF-α+) and IL-1b (CD11b+IL-1b+) in the spleen, spleen weight, and TNF-α secretion in HFD mice. Twenty-four mice were divided into six groups: normal (healthy mice); HFD (HFD mice without any treatment); HFD + Simvastatin (HFD mice receiving simvastatin); HFD + SBGE 100; HFD + SBGE 200; and HFD + SBGE 400 (HFD mice receiving 100, 200, and 400 mg/kg BW of SBGE for 4 weeks). Blood serum was collected at the end of treatment, and macrophage was isolated from the spleen. The relative number of CD11b+TNF-α+ and CD11b+IL-1b+ were examined using flow cytometry. SBGE treatment significantly (p<0.05) reduced the spleen weight and the relative number of CD11b+TNF-α+ and CD11b+IL-1b+ in the spleen of HFD mice. SBGE treatment also prevents the elevation of TNF- α levels in the blood serum. The optimal dose of SBGE to diminish the relative number of CD11b+TNF-α+, CD11b+IL-1b+ in the spleen, and TNF-α in the serum was 100 mg/kg BW.
POTENSI EKSTRAK ETANOL LABU SIAM (Sechium edule) UNTUK PERBAIKAN KERUSAKAN SEL BETA PANKREAS DAN KADAR NITROGEN OKSIDA PADA TIKUS YANG MENGALAMI DIABETES MELITUS (The Potenty of Sechium edule Ethanolic Extract to Repair Beta Pancreas Cells and Nitrogen Oxide Concentration in Streptozotocin-induced Diabetic Rat) Betty Lukiati; Siti Imroatul Maslikah; Nugrahaningsih N
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (425.589 KB) | DOI: 10.21157/j.ked.hewan.v10i1.3365

Abstract

The objectives of this research were to examine the effect of Sechium edule ethanolic extract to repair beta pancreas cells damage and tomeasure nitrogen oxide (NO) concentration in streptozotocin-induced rats. Type I diabetic rats were obtained by intraperioneally injected therats with multiple low dose streptozotocin (MLD-STZ) for 5 days. In this research, rats were assigned into 5 treatment groups: untreated rats ingroup 1 were considered as negative control, while MLD-STZ induced type 1 diabetic rats in group 2 were considered as positive control. Inanother 3 groups, type 1 diabetic rats were orally treated with three doses of Sechium edule ethanolic extract (14, 28, 42 mg/kg bw) respectivelyfor 7 consecutive days. Repairing of beta pancreas cells were analyzed descriptively whereas concentration of NO were analyzed using one wayANOVA and continued with LSD test (α= 0.01). The result showed that the administration of Sechium edule ethanolic extract significantlydecreased NO concentration (P0.01) up to 67.9 µM and repaired beta pancreas cells damage. Dose of 42 mg/kg bw of Sechium edule ethanolicextract is the effective dose for diabetic rat therapy. Key words: Sechium edule extract, diabetic mellitus, rat, beta pancreas cells
MICROBIOLOGICAL QUALITY OF CAYENNE PEPPER (Capsicum frutescens L.) IN MALANG TRADITIONAL MARKET Abdul Fattah Noor; Utami Sri Hastuti; Betty Lukiati
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 10 No. 1 (2023): August
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v10i1.9391

Abstract

Cayenne pepper is consumed as a food spice because of its color, spicy taste, aroma and complete nutritional value. Cayenne pepper sold in traditional markets is not separated between whole cayenne pepper and broken cayenne pepper. This study aims to: 1) calculate the ALT of mold colonies on whole and damaged cayenne peppers, 2) identify contaminant mold species found in intact and damaged cayenne peppers. Samples of whole cayenne pepper and damaged cayenne pepper were isolated by grinding 10 grams of cayenne pepper, it was then inoculated on Potato Dextrose Agar (PDA) medium and incubated at 25oC for 7 x 24 hours. Then the ALT of mold colonies was calculated on whole and damaged cayenne pepper. Then, each isolated mold contaminating colony's morphology and microscopic characteristics were described. Finally, each contaminant mold isolate was identified. Furthermore, the study unpacked that: 1) The ALT of contaminant mold colonies on whole cayenne pepper was 1.0x103 colonies/g, while the average ALT of contaminant mold colonies on damaged cayenne pepper was 2.1x105 colonies/g, 2) There were 8 species of mold found in cayenne pepper namely Mucor racemosus Fres, Aspergillus niger van Tieghem, Penicillium griseofulvum Dierckx, Penicillium frequentans Westing, Penicillium citrinum Thom, Penicillium nalgiovense Laxa, Penicillium digitatum Sacc and Penicillium variabile Sopp.
Collaborative mind mapping in RICOSRE learning model to improve students’ information literacy Liah Badriah; Susriyati Mahanal; Betty Lukiati; Murni Sapta Sari
International Journal of Evaluation and Research in Education (IJERE) Vol 13, No 1: February 2024
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijere.v13i1.26840

Abstract

This research aimed to determine how the reading, identifying problem, constructing solution, solving problem, reviewing and extending problem solution (RICOSRE) learning model integration with collaborative mind mapping affects students’ information literacy in higher education institutions. This quasi-experimental study used a pretest-posttest non-equivalent control group design. There were 40 multiple-choice questions selected to assess student information literacy. All instruments have been validated and are reliable. The data were collected from 100 participants who were college students enrolled in the environmental pollution course of the biology education degree program at Siliwangi University, Indonesia. To analyze the research data, analysis of covariance (ANCOVA) was used at a 5% level of significance, followed by the least significance difference (LSD) test. The ANCOVA results showed that the RICOSRE-CMM learning model impacted students’ information literacy. LSD scores were significantly different in terms of students’ information literacy. It can be concluded that the RICOSRE-CMM model could enhance each component of information literacy as these components have been integrated into the learning model syntax, which involves a series of information discovery and analysis processes during group work to find a solution to a problem.
Uji Daya Antibakteri Ekstrak Biji Pala Myristica fragans Houtt Varietas Tidore 1 Terhadap Bakteri Bacillus subtilis dan Escherichia coli Secara In Vitro Serta Analisis Kandungan Beberapa Senyawa Aktif Antibakteri Nursyahbani Saraha; Utami Sri Hastuti; Betty Lukiati
Jurnal Ilmu Hayat Vol 3, No 1 (2019)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um061v3i12019p13-21

Abstract

Diare merupakan salah satu penyakit saluran pencernaan makanan yang sering menimbulkan masalah yang terjadi di masyarakat Tidore. Penyakit tersebut disebabkan oleh bakteri Bacillus subtilis dan Escherchia coli. Biji pala dapat digunakan untuk mengobati penyakit diare. Senyawa antibakteri yang terkandung dalam biji pala adalah: flavonoid, tanin, alkaloid, fenolat, dan terpenoid. Penelitian ini bertujuan untuk menguji pengaruh ekstrak biji pala Tidore 1 dalam beberapa macam konsentrasi terhadap penghambatan pertumbuhan Bacillus subtilis dan Escherchia coli, menentukan konsentrasi ekstrak biji pala yang paling efektif terhadap penghambatan pertumbuhan bakteri Bacillus subtilis dan Escherchia coli, serta Menganalisis kandungan beberapa senyawa antibakteri dalam biji pala Tidore 1. dengan konsentrasi ekstrak yang digunakan yaitu: 1%, 3%, 5%, 7%, dan 9% dengan pelarut aquades steril. Hasil penelitian menunjukkan Konsentrasi ekstrak biji pala 7% merupakan konsentrasi yang paling efektif menghambat pertumbuhan bakteri Bacillus subtilis dan konsentrasi ekstrak biji pala 5% merupakan konsentrasi yang paling efektif menghambat bakteri Escherchia coli  
Uji Aktivitas Antibakteri Ekstrak Metanol Kulit Buah Apel Manalagi (Malus sylvestris Mill.) Terhadap Bakteri Staphylococcus epidermidis secara In Vitro Nazilatul Khoiroh; Betty Lukiati; Sitoresmi Parabaningtyas
Jurnal Ilmu Hayat Vol 2, No 1 (2018)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um061v2i12018p34-44

Abstract

Limbah kulit apel manalagi belum banyak dimanfaatkan. Limbah kulit apel memiliki kandungan senyawa aktif yang lebih banyak dari pada  buahnya (Wolfe, 2003). Kandungan senyawa aktif  limbah kulit apel manalagi dapat dimanfaatkan sebagai antibakteri pada bakteri Staphylococcus aureus dan Escherichia coli (Albertoet al., 2006). Penelitian ini bertujuan untuk menentukan senyawa aktif yang terkandung dalam ekstrak metanol kulit buah apel manalagi (Malus sylvestris Mill) dan menentukan konsentrasi ekstrak metanol kulit buah apel manalagi (Malus sylvestris Mill) yang paling efektif dalam menghambat pertumbuhan bakteri Staphylococcus epidermidis berdasarkan Kadar Hambat Minimum (KHM) dan Kadar Bunuh Minimum (KBM). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 2 kelompok control dan 5 kelompok perlakuan yang masing-masing 4 ulangan. Kontrol negatif tanpa pemberian ekstrak dan kontrol positif menggunakan kloramfenikol50 µg/ml. lima perlakuan konsentrasi ekstrakyaitu: 20.000 µg/ml, 40.000 µg/ml, 60.000 µg/ml, 80.000 µg/ml, dan 100.000 µg/ml. Pengumpulan data dilakukan dengan mengukur Kadar Hambat Minimum (KHM) dan Kadar Bunuh Minimum (KBM) bakteriStaphylococcus epidermidis. Teknik analisis data dilakukan dengan menggunakan program pengolah data uji non parametrik Krusskal Wallis dan dilanjutkan dengan uji lanjut Mann-Whitney. Hasil penelitian menunjukkan bahwa ekstrak metanol kulit buah apel manalagi mengandung senyawa aktif diantaranya flavonoid, terpenoid, polifenol, tanin,dan saponin. Konsentrasi ekstrak metanol kulit buah apel manalagi yang paling efektif dalam menghambat dan membunuh bakteri Staphylococcus epidermidis pada konsentrasi 60.000 µg/ml.
Karakterisasi Fitokimia Ekstrak Metanol Ental dan Rhizoma Pteris linearis Poir Zauhara Faiqohtun Wuriana; Betty Lukiati; Eko Sri Sulasmi
Jurnal Ilmu Hayat Vol 3, No 2 (2019)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um061v3i22019p64-71

Abstract

Tumbuhan menghasilkan sejumlah besar produk alami (metabolit sekunder). Dalam dekade terakhir, banyak dilakukan penelitian mengenai dampak positif dari metabolit sekunder yaitu sebagai obat herbal. Salah satu tumbuhan yang dapat berpotensi sebagai bahan obat herbal adalah Pteris linearis Poir. Salah satu hutan di Indonesia yang diketahui terdapat Pteris linearis adalah Taman Nasional Baluran (TNB). Kondisi lingkungan yang berbeda akan mempengaruhi macam dan kadar metabolit sekundernya. Selain itu, produksi metabolit sekunder pada organ, jaringan dan sel sangat spesifik. Senyawa ini sering ditemukan berbeda dalam satu individu tumbuhan. Tujuan penelitian ini karakterisasi metabolit sekunder pada organ yang berbeda yaitu ental dan rhizoma Pteris linearis di Taman Nasional Baluran. Ekstraksi tumbuhan dengan metode maserasi. Karakterisasi senyawa ini secara kualitatif dengan metode skrining fitokimia. Hasil penelitian ekstrak ental mengandung senyawa polifenol, flavonoid, terpenoid dan alkaloid, ekstrak rhizoma mengandung polifenol dan alkaloid. Simpulan penelitian ini adalah Pteris linearis berpotensi sebagai bahan obat herbal, namun ekstrak ental lebih efektif dibandingkan ekstrak rhizoma.
Development e-module on human reproduction system materials science-Islam integrated to improve science literacy and student learning outcomes Siti Aisyah Firdani; Abdul Gofur; Betty Lukiati
BIO-INOVED : Jurnal Biologi-Inovasi Pendidikan Vol 6, No 1 (2024): February 2024
Publisher : Master Program of Biology Education, Universitas Lambung Mangkurat, Banjarmasin, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/bino.v6i1.17762

Abstract

Low scientific literacy is due to the large amount of material being taught and not applying basic biological concepts to problems commonly encountered in the surrounding environment. The Islamic science integration approach, which was developed into teaching materials in the form of e-modules, needs to be applied because it places a variety of diversity in scientific disciplines (Islamist studies, Natural studies, Social studies, and Humanities). The research aims to develop and test an E-module's validity, practicality, and effectiveness based on Problem-Based Learning (PBL) Integrated Islamic Science Human Reproductive System Material to Improve Science Literacy and Student Learning Outcomes. The research and development model refers to a Branch consisting of ADDIE, namely the analyze, design, develop, implement, and evaluate stages. The data collection techniques used were questionnaires and validation sheets. The results of research on the effectiveness of e-modules show that e-modules are very valid in improving scientific literacy and student learning outcomes. Based on the N-gain calculation, scientific literacy is in the medium category with significant t-test results. The learning outcomes are in the high category with very significant t-test results. This research shows that the PBL-based e-module material on the science-Islam integrated human reproductive system can be developed and effectively used in learning activities.Abstrak. Literasi sains rendah disebabkan banyaknya materi yang diajarkan, kurang menerapkan konsep dasar biologi terhadap permasalah yang biasa ditemui di lingkungan sekitar. Pendekatan integrasi sains-Islam yang dikembangkan menjadi bahan ajar berupa e-module perlu diterapkan karena menempatkan berbagai keberagaman terhadap disiplin ilmu (Islamis-Studies, Natural studies, Social studies dan Humaniora). Penelitian bertujuan untuk mengembangkan, menguji kevalidan, kepraktisan, dan keefektifan e-module berbasis Problem Based learning (PBL) Materi Sistem Reproduksi Manusia Terintegrasi Sains Islam untuk Meningkatkan Literasi Sains dan Hasil Belajar Mahasiswa. Model penelitian dan pengembangan mengacu pada Branch terdiri dari ADDIE yaitu tahap analyze, design, develop, implement, dan evaluate. Tenik pengumpulan data yang digunakan berupa angket dan lembar validasi. Hasil penelitian terhadap keefektifan e-module menunjukkan bahwa e-module sangat valid dalam meningkatkan literasi sains dan hasil belajar mahasiswa. Berdasarkan perhitungan N-gain literasi sains masuk kategori sedang, dan hasil belajar masuk kategori tinggi dengan hasil uji-t yang sangat signifikan. Hasil penelitian ini dapat disimpulkan bahwa e-module berbasis PBL materi sistem reproduksi manusia terintegrasi sains-Islam dapat dikembangkan dan efektif digunakan dalam kegiatan pembelajaran.
Bioinformatics approach to enhance the undergraduate biology students’ understanding of plant terpenoid Risanti Dhaniaputri; Hadi Suwono; Betty Lukiati
JPBI (Jurnal Pendidikan Biologi Indonesia) Vol. 10 No. 2 (2024): JULY
Publisher : University of Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jpbi.v10i2.33884

Abstract

Plant metabolite compounds have been applied on plant cellular metabolism, produces organic and inorganic compounds, primary and secondary bioactive molecules, such as glucose, amino acids, fat acids, alkaloids, flavonoids, and terpenoids. Undergraduate biology students learn about the plant terpenoid assisted by bioinformatics to store, manage, and interpret the molecular information about these compounds. This research aims to observe the students’ understanding of terpenoid through implementing bioinformatics approach. Besides, to investigate how practice the bioinformatics technology in learning terpenoid may impact students' comprehension of plant metabolism domain and their acquisition of bioinformatics inquiry skills. Bioinformatics is a computational database that relies on digital repositories of molecular biology informations. Data analysis was in the form of quantitative and qualitative descriptive using module learning resources based on terpenoid research and assisted by bioinformatics. The results indicate that students’ comprehension of learning and understanding terpenoid has improved, identification and analysis processes of article reviews showed that students were able to discuss and interpret research finding in silico bioinformatics using molecular docking procedures. Assessment of bioinformatics skills showed that all undergraduate biology students could follow the direct instructions well, answer the questions, practice the dry-lab experimental, and formulate the conclusion correctly.
Enacting Life-Based Learning (LBL) Approach in Quantitative Research Methodology Course: The Case of Biology Education Students Susilo, Herawati; Lestari, Sri Rahayu; Lukiati, Betty; Sudrajat, Ahmad Kamal
JPP (Jurnal Pendidikan dan Pembelajaran) Vol 26, No 2 (2019)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um047v26i22019p066

Abstract

This study portrayed the enactment of Life-Based Learning (LBL) in quantitative research methodology course among Master’s students in biology education. Literature study and action research were employed to capture data. The literature study attempted to unveil how LBL was performed. Furthermore, the action research was in the form of Lesson Study (LS) to carry out the course outline. Practically, characteristics of LBL were performed using authentic materials. These were in the forms of filling out questionnaire of first semester intake, doing peer L, developing mind/concept mapping, assessing peers’ mind/concept mapping, assessing peer portfolio, writing learning journal, doing critical analysis on journal articles, developing research proposal, assessing peers’ critical analysis and performance, uploading files of mind/concept mapping, and presenting critical analysis works. From the action research lens, different-class students preferred varied strategies for similar learning materials and they favored such processes.
Co-Authors Abdul Fattah Noor Abdul Gofur Abdul Gofur AD. Corebima Agus Muji Santoso Alif Rofiqotun Nurul Alimah Alvina Putri Purnama Sari Alvina Putri Purnama Sari Anindya Nirmala Permata Anindya Nirmala Permata Arwinda Probowati Atik Kurniawati Balqis balqis Bayu Guruh Binangkit Dian Eka A. F. Ningrum Dianing Eka Pupitasari Dina Aribah Dina Maulina DYAH ASTRIANI Eko Sri Sulasmi Endang Suarsini Fatchur Rohman Fathul Zannah Fitriani, Rita Frida Kunti Setiowati Hadi Suwono Hanzen, W.F Edi Hendra Susanto Hera Adiwijaya Herawati Susilo Ibrohim Ida Rahmawati Ihya Fakhrurizal Amin Jeni Jeni Jeni Jirana, Jirana Khaq, Rizki Amalia Nurul Kurniawan Setia Putra Liah Badriah M. Amien Rais M. Saiful Fahmi M. Saiful Fahmi, M. Saiful Fahmi Mariana Ade Cahaya Miftahul Mufinadiroh Mimien Henie Irawati Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohammad Amin Mohammad Amin Mohammad Amin Mohammad Amin Muhamad Khoirul Anwar, Muhamad Khoirul Muhammad Khalil Muhammad Saefi Muhammad Shobirin Mumun Nurmilawati Murni Sapta Sari Muslihasari, Adzimatnur Nanda Earlia Nazilatul Khoiroh Nelda Anasthasia Serena Ningrum, Dian Eka A. F. Novia Sigma Amalina Nugrahaningsih N, Nugrahaningsih Nugrahaningsih Nugrahaningsih Nur Fitriana Nur Fitriana Nursyahbani Saraha Poppy Rahmatika Primandiri Puspitasari, Dianing Eka Putri Diyah Anggraini Putri Purnama Sari, Alvina Putri Purnama Sari, Alvina Risanti Dhaniaputri Sembiring, Rinawati Serena, Nelda Anasthasia Siti Aisyah Firdani Siti Imroatul Maslikah Siti Nur Arifah Sitoresmi Parabaningtyas Sitoresmi Prabaningtyas Solly Aryza Sri Rahayu Lestari Sulistiono Sulistyo Dwi Kartining Putro Sulistyo Dwi Kartining Putro, Sulistyo Dwi Kartining Susriyati Mahanal Sutiman Bambang Sumitro Tahir, Jirana Triastono Imam Prasetyo Umi Hasanah, Umi Umie Lestari Utami Sri Hastuti W. F. Edi Hanzen W.F Edi Hanzen Widodo Widodo Yayoi Kodama Yessi Hermawati Zauhara Faiqohtun Wuriana