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Journal : Bionature

Vegetative Growth Response of Corn (Zea mays) to Actinomycetes, Azospirillum, and Azotobacter Isolated from the Rhizosphere of Neem (Azadirachta indica) Yusminah Hala; A. Irma Suryani; Octavia Cisiliana Devi
Bionature Vol. 24 No. 1 (2023): April 2023
Publisher : Jurusan Biologi Universitas Negeri Makassar

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Abstract

This research is an experimental study that aims to determine the effect of the application of N2-fixing bacteriaActinomycetes, Azospirillum, and Azotobacter on the vegetative growth parameters of corn plants and on thenitrogen content of corn plants. Parameters for measuring the growth of corn plants include: formation rate ofradicle length, plant height, number of leaves, leaf length, fresh weight of plants, fresh weight of plant roots, dryweight of plants, dry weight of plant roots, total N content in plants, as well as physical and chemical qualities ofthe ground. Experiments on the application of N2 -fixing bacteria on corn plants on a laboratory scale were carriedout to determine the in vitro effect of microbes on corn seeds. The pot test was carried out to determine the effectof N2-fixing bacteria on the vegetative growth of corn plants in vivo. Data were analyzed using ANOVA withDuncan's test. The results showed that corn plants inoculated with Actinomycetes, Azospirillum and Azotobactershowed a significant different growth from corn plants without nitrogen fixing bacteria, namely formation rate ofradicle length, plant height, number of leaves, leaf length, fresh weight, dry weight, total N content (%) of crops,and soil physical and chemical quality. It can be concluded that the inoculation of nitrogen-fixing bacteria in cornplants has a significant effect on plant vegetative growth parameters and plant nitrogen content.
Indole acetic acid (IAA) product Indole acetic acid (IAA) production by endophytic bacteria from Stem And Roots Of Indian Ash Tree (Lannea Coromandelica): Indian Ash Tree (Lannea Coromandelica) Hala, Yusminah; Ali, Alimuddin; Suryani, A. Irma
Bionature Vol. 25 No. 2 (2024): Oktober-Desember 2024
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v25i2.4618

Abstract

The endophytic bacteria live in healthy plants and can even improve plant health and productivity by suppressing pathogenic bacteria and increasing plant growth due to the producing IAA hormone. The purpose of this study was to describe the ability of each bacterial isolate to produce IAA hormone, to determine the fluctuations that occur during the five-day incubation period. The endophytic bacterial isolates from the roots of the Indian ash tree are A1 Bacillus, A2 Bacillus, A3 Bacillus, A4 Pseudomonas and A5 Bacillus, while from the stems are B1 Bacillus, B2 Agrobacterium, B3 Bacillus, B4 Bacillus and B5 Agrobacterium. The potential of bacterial isolates in producing IAA hormone was obtained by measuring the absorbance value using a spectrophotometer at a wavelength of 530 nm which was carried out every day for five days of incubation. The results obtained showed that endophytic bacterial isolates from the roots and stems of Kayu Jawa Plants have the ability to produce IAA with the highest concentration on 3 days of incubation was produced by isolate A3 (7.95 ppm), from the genus Bacillus, on 4 days of incubation the highest concentration was produced by A2 (22.18 ppm) from the genus Bacillus and on 5 days of incubation the highest concentration was produced by A2 (57.60 ppm) from the genus Bacillus.