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PENGARUH PENGELOLAAN AIR DAN GENOTIPE PADI TERHADAP KERACUNAN BESI DAN PRODUKTIVITAS PADI DI LAHAN PASANG SURUT TIPE LUAPAN B DI SUMATERA SELATAN Harahap, Siti Maryam; Ghulamahdi, Munif; Aziz, Sandra Arifin; Sutandi, Atang; Miftahudin, ,
Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Vol 17, No 2 (2014): Juli 2014
Publisher : Jurnal Pengkajian dan Pengembangan Teknologi Pertanian

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Abstract

The Effect of Water Management and Rice Genotypes to Against Toxicity of Iron and Productivity of Rice in Tidal Land Type B in South Sumatera. Tidal land is one of the alternative land to support increased productivity of rice, but rice productivity in this area is still low, and the land utilization is not optimal yet. One factor is high iron content in the soil that can be toxic to plants. The purposes of this study were (1) to determine the effect of water management and rice genotype, as well as their interaction on the percentage of leaf bronzing, growth and yield of rice in tidal lands, (2) to select an alternative combination of water management and rice genotypes that may increase rice yields in tidal land. This study was conducted from February until June 2013 in the tidal area with type B overflow, Mulia Sari village, Banyu Asin regency, South Sumatera. The experimental design used was a split plot design with three replications. The main plot was water management with four levels, while the subplot was rice genotypes consisted of three genotypes. The results showed that water management and its interactions with plant genotypes had significant effect only on the percentage of bronzing, Fe content in shoot, and Fe content in root crops, while the plant genotype significantly affected all observed variables. The use of genotypes tolerant to iron toxicity (Indragiri) combined with (form stagnant and saturated water management) drainage with intervals of two weeks could give yield 6.8 and 6.2 t/ha respectively.Keywords : Water management, genotype paddy, tidal landABSTRAKLahan pasang surut merupakan salah satu lahan alternatif untuk mendukung peningkatan produksi padi, tetapi produksi padi di lahan ini masih rendah, dan pemanfaatan lahannya belum optimal. Salah satu faktor penyebabnya adalah adanya senyawa besi dalam tanah yang dapat meracuni tanaman. Tujuan penelitian ini adalah (1) untuk mengetahui pengaruh pengelolaan air dan genotipe tanaman padi serta interaksinya terhadap hasil padi di lahan pasang surut, (2) memilih alternatif kombinasi pengelolaan air dan genotipe tanaman padi yang sesuai dan dapat meningkatkan hasil padi di lahan pasang surut. Penelitian ini dilaksanakan bulan Februari – Juni 2013 di lahan pasang surut tipe luapan B, Desa Mulia Sari, Kabupaten Banyu Asin Sumatera Selatan. Rancangan yang digunakan adalah Rancangan Petak Terpisah dengan tiga ulangan. Sebagai petak utama adalah pengelolaan air, yang terdiri dari empat taraf, sedangkan sebagai anak petak adalah genotipe padi, yang terdiri dari tiga genotipe (IRH108, IR64 dan Indragiri). Hasil penelitian menunjukkan bahwa pengelolaan air dan interaksinya dengan genotipe tanaman berpengaruh nyata terhadap persentase bronzing, kandungan Fe di tajuk, kandungan Fe di akar dan hasil tanaman sedangkan genotipe tanaman berpengaruh nyata terhadap semua peubah yang diamati. Penanaman genotipe tanaman toleran terhadap keracunan besi (genotipe Indragiri) yang dikombinasikan dengan drainase berinterval dua minggu dapat memberikan hasil masing-masing 6,8 dan 6,2 t/ha.
IDENTIFIKASI GEN / QTL (Quantitative Trait Loci) SIFAT TOLERAN CEKAMAN ALUMINIUM PADA GALUR-GALUR PADI GOGO [Identification of Gene/QTL (Quantitative Trait Loci) for Aluminium Stress Tolerant in Upland Rice Lines] Utami, Dwinita Wikan; Rosdianti, I; Yuriyah, S; Ambarwati, AD; Hanarida, I; Suwarno, Suwarno; Miftahudin, Miftahudin
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i2.2276

Abstract

Program on the ability of agriculture to provide rice as a staple food tends to decrease with the reduced land area used for rice cultivation. improvement rice production should be directed to use  marginal land  as dry and acid land, where poisoning aluminium was seen as a constrain factor. Development of Aluminium (Al) tolerant variety is needed to increase rice production. This technology was environmentally friendly, cheaper and also easier for farmers adopted. The rice lines with broad genetics background which tolerant to Al toxicity has been produced by breeding program. The diversity of Indonesian local rice germplasm is a basic foundation on development of Al toxicity tolerant rice varieties. The purpose of this research was to analyze the genotype variety using 384 SNPs markers on selected Al tolerant rice lines based on bioinformatics approach. Based on the 384 SNPs markers, the total 26 selected  genotypes were clustered into 5 groups. Each group was having varied Relative Root Length (RRL), which was an Al tolerant parameter of phenotype. These groups were indicated for having different genes or QTL that were related to the Al tolerant character. The association analysis obtained 9 significant SNPs markers that can be applied as the molecular markers for assisting selection in breeding  program.
Morphological Diversity of Local Sorghum Cultivar (Sorghum bicolor) of East Nusa Tenggara, Indonesia Tnunay, Ite Morina Yostianti; Chikmawati, Tatik; Miftahudin, Miftahudin
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (701.916 KB) | DOI: 10.15294/biosaintifika.v11i1.15199

Abstract

Sorghum has great potential to be developed in marginal lands of Indonesia including East Nusa Tenggara. However, the information about sorghum diversity in this area was very limited. This research aimed to describe morphological variation, and assessing the value of genetic diversity based on morphological characters of local sorghum cultivar of East Nusa Tenggara. The exploration and sample collection were conducted in 3 islands, i.e.: Timor, Sumba, and Flores. The observation of morphological characters was based on sorghum descriptor. Morphological character similarities were analyzed using Simple Matching (SM) coefficient, and a dendrogram was constructed using Unweighted Pair Group Method with Arithmetic Average (UPGMA) method. The results showed that 36 accessions of sorghum in East Nusa Tenggara varied in 17 morphological characters. At the similarity coefficients of 48%, all sorghum accessions were separated into 2 groups based on the presence or absence of aleurone layer. Group I consisted of 12 accessions with no aleurone layer, while group II consisted of 24 accessions has aleurone layer. The genetic diversity of sorghum of East Nusa Tenggara is low (I=0.62–0.71 and h=0.37-0.42). The genetic diversity between populations (HT=0.4203) is higher than within populations (HS=0.3961). This study provides the first complete information about sorghum diversity in East Nusa Tenggara that will be useful as basic information for sorghum development in this area in the future.
Karakter Root Re-Growth Sebagai Parameter Toleransi Aluminium pada Tanaman Padi Roslim, Dewi Indriyani; Miftahudin, Miftahudin; Suharsono, Utut; Aswidinnoor, Hajrial; Hartana, Alex
Jurnal Natur Indonesia Vol 13, No 1 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.048 KB) | DOI: 10.31258/jnat.13.1.82-88

Abstract

Aluminum (Al) is one of the major limited factors in crop production on acid soils. Aluminum tolerant plants can beselected from plant breeding program by one of the physiological parameters representing Al tolerance character,such as root re-growth capability during recovery from the Al-stress. In this study we determined the concentrationand time exposure of Al stress that was able to differentiate the response of three local upland rice varieties(Grogol, Hawarabunar and Krowal) and an Al-sensitive rice variety (IR64) to Al-stress, and evaluated the effectivenessof root re-growth (RRG) characters as an Al tolerance parameter in rice. The study consisted of three experiments,which were 1) nutrient culture experiment with different Al concentration treatments in growth chamber, 2) potexperiment in greenhouse using Jasinga yellow red podzolic acid soil containing 26,66 me/100 g Al and pH 4,6 asplanting media, and 3) phenotyping of F2 population using RRG character. The results showed that Al treatment at15 ppm for 72 h was able to distinctly differentiate between Al-tolerant (Grogol and Hawarabunar) and Al-sensitivevarieties (Krowal and IR64). Planting of the rice varieties on acid soils showed similar result as that of the nutrientculture. Phenotyping of F2 population using RRG character indicated the existence of RRG value variation. Thesevariations demonstrated that RRG character can be used as an Al tolerance parameter in rice and therefore can beeffectively applied to screen rice F2 population that segregate to Al tolerance character.
KERAGAMAN UBI KAYU (Manihot esculenta Crantz.) HASIL PERBANYAKAN IN VITRO BERDASARKAN KARAKTER MORFOLOGI DAN PENANDA ISSR Hartanti, Fajri; Miftahudin, Miftahudin; Hartati, N Sri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1494.884 KB) | DOI: 10.29122/jbbi.v6i2.3055

Abstract

Morphological and Molecular Diversity of Cassava (Manihot esculenta Crantz) Resulted from In Vitro PropagationIn vitro propagation of cassava (Manihot esculenta Crantz) on medium containing plant growth regulator (PGR) may induce morphological variation. This study aimed to analyze the morphological and genetic diversity of 13 genotypes of cassava resulted from in vitro propagation and stem cuttings based on 11 vegetative characters and 7 ISSR markers. Morphological and genetic characters were scored and used for clustering using NTSYS-pc 2.11a. Roti control and Adira 4 control genotypes that were in vitro propagated without PGR addition showed different morphological characters with Roti variant and FEC-25 genotypes that were in vitro propagated with the addition of PGR. Morphological and molecular characters of 13 genotypes showed high diversity. Clustering analysis based on morphological characters classified the in vitro propagated and control plants into four groups at 45.6% similarity. Clustering analysis based on molecular characters classified the plants into three groups at 66.0% similarity.Keywords: cassava; diversity; in vitro; ISSR; morphologyABSTRAKPerbanyakan tanaman ubi kayu (Manihot esculenta Crantz) secara in vitro menggunakan zat pengatur tumbuh (ZPT) diyakini dapat menginduksi variasi morfologi. Tujuan dari penelitian ini adalah untuk menganalisis keragaman morfologi dan molekuler dari 13 genotipe ubi kayu hasil perbanyakan in vitro dan perbanyakan dengan stek batang berdasarkan 11 karakter vegetatif dan 7 penanda ISSR. Karakter morfologi dan molekuler diskor untuk analisis kelompok menggunakan program NTSYS-pc 2.11a. Genotipe Roti kontrol dan Adira 4 kontrol yang merupakan hasil perbanyakan in vitro tanpa penambahan ZPT menunjukkan perbedaan variasi morfologi dengan genotipe Roti varian dan FEC-25 yang merupakan tanaman hasil perbanyakan dengan penambahan ZPT. Hasil analisis pada 13 genotipe menunjukkan adanya keragaman yang tinggi. Hasil analisis kelompok berdasarkan penanda morfologi memisahkan antara genotipe hasil perbanyakan secara in vitro yang ditambah ZPT dengan tanaman kontrolnya ke dalam 4 kelompok dengan nilai koefisen similaritas 45,6%. Hasil analisis kelompok berdasarkan penanda molekuler memisahkan antara genotipe hasil perbanyakan secara in vitro yang ditambah ZPT dengan tanaman kontrolnya ke dalam 3 kelompok dengan nilai koefisen similaritas 66,0%.
Identifikasi Morfologi dan Marka Molekuler Terpaut Sifat Tidak Berbunga Jantan Pada Mutan Pisang Kepok Nettyani, Naipospos; Miftahudin, Miftahudin; Sobir, Sobir
Jurnal Hortikultura Vol 24, No 1 (2014): Maret 2014
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v24n1.2014.p23-31

Abstract

Salah satu masalah dalam pengembangan produksi pisang ialah penyakit darah. Infeksi penyakit ini dapat dikurangi dengan menanam pisang kepok mutan tidak berbunga jantan. Penerapan teknik kultur jaringan dapat menyediakan benih seragam secara cepat dan dalam waktu yang singkat. Namun, penggunaan teknik kultur jaringan dapat menginduksi variasi somaklonal. Oleh karena itu perlu dilakukan penelitian tentang stabilitas genetik dan keseragaman morfologi pada benih hasil kultur jaringan. Penelitian dilakukan dari Bulan Agustus 2012 sampai Mei 2013. Identifikasi morfologi pisang dilaksanakan di Kebun Koleksi PKHT Ciomas, Bogor. Analisis molekuler dilaksanakan di Laboratorium Molekuler PKHT. Penelitian bertujuan mempelajari karakter morfologi mutan pisang kepok Unti Sayang tidak berbunga jantan dan mengidentifikasi marka molekuler terpaut sifat tidak berbunga jantan. Identifikasi karakter morfologi dari 24 sampel tanaman dilakukan menggunakan panduan deskriptor dari International Plant Genetic Research Institute. Identifikasi marka  molekuler dilakukan berdasarkan teknik PCR, menggunakan 20 primer RAPD, 12 primer ISSR, serta primer gen Pistillata, dan Agamous yang didesain berdasarkan informasi database sekuen DNA Musa acuminata yang terdapat di gene bank. Hasil pengamatan menunjukkan tidak ada variasi morfologi antara tipe liar, tanaman mutan, dan tanaman mutan yang kembali berbunga jantan, kecuali ada atau tidaknya bunga jantan. Analisis PCR dari 24 sampel tanaman menggunakan 20 primer RAPD dan 12 primer ISSR berturut-turut menghasilkan 379 dan 216 pita yang seragam. Hal yang sama juga ditemukan dari hasil amplifikasi DNA menggunakan primer Pistillata dan Agamous yang menghasilkan pita tunggal pada semua sampel. Analisis sekuen fragmen PCR hasil amplifikasi dengan primer gen Pistillata menunjukkan terdapat tiga nukleotida yang berbeda antara tipe liar dan tanaman mutan yang kembali berbunga jantan pada posisi 445, 461, dan 507.
The expression of OsPLA2-III and OsPPO genes in rice (Oryza sativa L.) under Fe toxicity stress Turhadi, .; Hamim, .; Ghulamahdi, Munif; Miftahudin, .
Journal of Tropical Life Science Vol 11, No 2 (2021)
Publisher : Journal of Tropical Life Science

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Abstract

Lipid is an important biomolecule in plant because of their structural and functional roles in plant cells. Moreover, lipid could be a signal molecule in defense system of plants under biotic and abiotic stresses. Detoxification process of toxic substances, which is one of the defense system and controlled by various genes, plays an important role in tolerance mechanism in plant under stress conditions, including iron (Fe) toxicity. Plants develop various tolerance strategies to cope the Fe toxicity. The objective of this research was to investigate the expression of OsPLA2-III and OsPPO genes under Fe stress condition. Two-weeks-old seedlings of two rice varieties, i.e., IR64 (Fe-sensitive toxicity) and Pokkali (Fe-tolerant toxicity) were used. Both varieties were treated with 1 (control) and 400 ppm of Fe in the form of FeSO4.7H2O in the nutrient culture solution. The leaf bronzing, chlorophyll content, and relative expression of OsPLA2-III and OsPPO genes were observed. An in-silico study was also performed to predict the interaction between OsPLA2-III and OsPPO proteins. The results showed that the Fe toxicity induced leaf bronzing, reduced leaf chlorophyll content, and increased the OsPLA2-III and OsPPO gene expression levels. Both genes are suggested to have a role in plant tolerance mechanism during Fe toxicity stress via lipid signaling pathway.
Pertumbuhan dan analisis kualitatif tanin, saponin dan flavonoid dari Selaginella plana, S. willdenovii and S. mayeri pada tiga naungan berbeda (The growth and qualitative analysis of tannin, saponin and flavonoid in Selaginella plana, S. willdenovii an Chikmawati, Tatik; Sopyati, Puspita Dewi; Miftahudin, Miftahudin
JURNAL BIOS LOGOS Vol 3, No 1 (2013): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.3.1.2013.14503

Abstract

Abstrak Selaginella yang banyak dijumpai melimpah di Indonesia sangat  berpotensi sebagai sumber antioksidan alami. Kadar bahan bioaktif dalam tumbuhan  sangat tergantung pada lingkungan tumbuhnya, namun penelitian aspek budidaya dari Selaginella di Indonesia yang berkaitan dengan bahan bioaktif belum banyak dilakukan.  Penelitian awal tentang aspek budidaya Selaginella telah dilakukan khususnya untuk mengetahui naungan yang tepat bagi pertumbuhannya serta pengaruhnya terhadap kandungan bahan bioaktif. Penelitian ini menggunakan rancangan petak terpisah dengan naungan sebagai petak utama dan jenis Selaginella sebagai anak petak. S. plana, S. willdenovii dan S. mayeri diberi perlakuan naungan dengan paranet 40%, 65%, 80%, dan tanpa naungan. Uji kandungan tanin, saponin, dan flavonoid secara kualitatif diketahui dengan melakukan ekstraksi dengan alkohol. Pertambahan cabang total, pertambahan bobot basah total, bobot basah, dan bobot kering biomassa dipengaruhi secara nyata oleh naungan dan jenis Selaginella. Masing-masing jenis Selaginella memerlukan intensitas cahaya yang berbeda untuk mendukung pertumbuhannya. Semua jenis yang diuji juga mengandung kadar tanin, saponin, dan flavonoid berbeda secara kualitatif. Kata kunci:  Selaginella, naungan, tanin, saponin, flavonoid Abstract Selaginella found abandonly in Indonesia is very potensial as the source of natural antioxidant. The amount of bioactive material of Selaginella depends on the environment, but research on the amount of bioactive material correlated to the cultivated aspect has not been done in Indonesia. The preeliminary research of the cultivation of Selaginella was  investigated especially to know the right shading for the best growth of Selaginella. The right shading may also affect bioactive content of Selaginella. This research used split plot design with level of shading as main plot and species as sub plot. S. plana, S. willdenovii, and S. mayeri were grown under paranet with 40%, 65%, 80% shading, and non shading treatments. Bioactive compound was extracted using alcohol 70% and analyzed for tannin, saponin, and flavonoid contents. Results indicated that level of shading and species affected total branch increase, total fresh weight increase, fresh and dry weight of biomass.  Each species required different light intensity to support its growth. All species in the experiment contained different qualitative level of tannin, saponin, and flavonoid. Keywords: Selaginella, shade, tanin, saponin, flavonoid
Evaluasi Galur-galur Padi Pup1 terhadap Cekaman Kekeringan Wardoyo, Suwaji Handaru; Miftahudin, Miftahudin; Moeljopawiro, Sugiono; Prasetiyono, Joko
Jurnal Penelitian Pertanian Tanaman Pangan Vol 1, No 1 (2017): April 2017
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.111 KB) | DOI: 10.21082/jpptp.v1n1.2017.p21-28

Abstract

Drought, acidity, and P deficiency are problem in rice production in Ultisol soil in Indonesia. Pup1 locus in rice plant, besides has a role in uptaking P nutrient, was suspected to improve resistant to biotic and abiotic stresses, including drought. BC2F6 lines derived from Situ Bagendit cross containing Pup1 locus had been developed through Marker-assisted Backcrossing method, and had been tested for P fertilizers and resistance to blast. This research was aimed to evaluate the effect of Pup1 locus to drought stress condition. The study was divided into two experiments, namely test of polyethylene glycol (PEG) 8000 and test of root penetration ability test (DTA). The experiment was conducted at the Greenhouse of Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, from November 2012 until May 2013. The number of lines used in this study was 52 lines, consisted of 24 lines of BC2F6 Situ Bagendit × Kasalath (= SK), 22 lines of Situ Bagendit × NIL-C433 (= SN), three parents namely Situ Bagendit (recurrent parent), Kasalath, and NIL-C433, two tolerant controls for drought namely Cabacu and Gajah Mungkur, and one sensitive control for drought IR20. The research used a completely randomized design for test PEG 8000 and a split-split plot design for test of root penetration ability.  Results showed that maximum PEG 800 concentrations for drought stress study on rice was 20%. Pup1 locus was more tolerant to drought stress in PEG 8000 experiment through its longer roots and plumules. Pup1 locus gave a positive effect on root penetration ability, with longer roots and more penetrating wax layer. Pup1 locus also showed tolerant to drought on Ultisols soil, as indicated by an increasing dry weight of root and shoot than those of parent without Pup1 locus.
Uptake and Distribution of Aluminum in Root Apices of Two Rice Varieties under Aluminum Stress MIFTAHUDIN MIFTAHUDIN; NURLAELA NURLAELA; JULIARNI JULIARNI
HAYATI Journal of Biosciences Vol. 14 No. 3 (2007): September 2007
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.356 KB) | DOI: 10.4308/hjb.14.3.110

Abstract

Aluminum (Al) toxicity is the major limiting factor of plant growth and production in acid soils. The target of Al toxicity is the root tip, which affects mainly on root growth inhibition. The aim of this research was to study the uptake and distribution of Al in root apices of two rice varieties IR64 (Al-sensitive) and Krowal (Al-tolerant), which were grown on nutrient solution containing 0, 15, 30, 45, and 60 ppm of Al. The root growth was significantly inhibited in both rice varieties at as low as 15 ppm Al concentration. The adventive roots of both varieties showed stunted growth in respons to Al stress. There was no difference in root growth inhibition between both rice varieties as well as among Al concentrations. Al uptake on root apices was qualitatively and quantitatively analyzed. Histochemical staining of roots using hematoxylin showed dark purple color on 1 mm region of Al-treated root apices. Rice var. IR 64 tended to take up more Al in root tip than Krowal did. However, there was no statistically significant difference (p = 0.176) in root Al content of both varieties in response to different concentration and period of Al treatments. Al distribution in root apices was found in the epidermal and subepidermal region in both rice varieties. Based on those results, rice var. Krowal that was previously grouped as Al-tolerant variety has similar root growth and physiological response to Al stress as compared to Al-sensitive variety IR64. Key words: aluminum, uptake, distribution, root, rice
Co-Authors , Triadiati AD Ambarwati ADEEL ABDUL KARIM ALTUHAISH Afifah Azkiyah Ahmad Dwi Setyawan Ahmad Tarmidi Albarokah, Nurdiansyah ALEX HARTANA ALEX HARTANA Amarullah, Mohammad Syarifuddin Ambarwati, AD Anarki, Aprillia Alling Anjar Tri Wibowo Arafah, Purnama Aris Tjahjoleksono Atang Sutandi Awik Puji Dyah Nurhayati Aziz, Alvi Bachtiar, Machdum BUDI SETIADI DARYONO Budiarto, Despiyan Dwi CHAIDIR CHAIDIR Dasumiati Dasumiati Desvian Bandarsyah Devi Media Siska Dewi Indriyani Roslim DIANA AGUSTIN CAROLINA DIBYO PRONOWO Dwi Jatmoko Dwi Suci Setyaningsih Dwinita Wikan Utami DWINITA WIKAN UTAMI Eka Setiawan Fahrurrozi Fahrurrozi Faiz, Agus Maulana Fauzobihi, Fauzobihi Fuad Fitriawan, Fuad Habizar, Habizar Hafizhni Anggia, Rafi HAJRIAL ASWIDINNOOR Hamim Hamim Hanarida, I Harisca, Rodhi Hartanti, Fajri Hartati, N Sri Haryanti, Intisari HIMMAH RUSTIAMI Himmah Rustiami, Himmah I Hanarida I PUTU GEDE P. DAMAYANTO I PUTU GEDE P. DAMAYANTO, I PUTU GEDE P. I Rosdianti Ilham Dinar Akbar Cudino Iman Rusmana Iswandi Anas Isya, Mandanu Akhta Nur Iwan, Pristanto Ria Irawan J PERRY GUSTAFSON Joko Prasetiyono JULIARNI JULIARNI Juliyani Juliyani Kamini, Kamini Khoirurrijal Khoirurrijal, Khoirurrijal Komalasari, Selly Siti Komalasari Kustiariyah, Kustiariyah Kusumanegara, Kusumawaty LA KOLAKA Latifah, Vida Rahma M Ilham Fahmi Roji MARIA BINTANG Marlina Ardiyani Maududy, Robby Meitha, Karlia Moeljopawiro, Sugiono Muchozin, Muhammad Ni'an Muhammad Akbar Velayati Muhammad Alfarabi Mukhlash Abrar Munif Ghulamahdi Muslimin Muslimin MUTTAQIN, MAFRIKHUL Nadzar Saifudin Nasir, Fahad Muhammad Nasir Nettyani, Naipospos Nugroho, Giri Nunung Fajaryani, Nunung Nurlaela Nurlaela Nurlaela Nurlaela Nurlaila, Santi Nurlaila OKKY SETYAWATI DHARMAPUTRA Pieter Agusthinus Riupassa Polosoro, Aqwin Prasetiyono, Joko Purwanti, Tira Puspita Dewi Sopyati Putri Aulia Putro, Karso Suryo Raden Mohamad Herdian Bhakti RANTJE LILLY WORANG Rasyid, Aliy Ratnasari, Seli Risa Aryantri Rizal Nursamsi, Dede RIZAL SYARIEF Rosandi Ardi Noegraha Rosdianti, I Ruwirohi, Jan Everhard S Yuriyah Sahab, Fahmi Sahab Sairun Sairun Salimah, Hafshah Aqidatun Sandra Arifin Aziz Sartika, Dian Setiawan Setiawan Setiawan, Jeffry SISWA SETYAHADI Siti Maryam Harahap Slamet, Oky Sobir Sobir Sokoy, Mercy Sopandi, Nur Irfan Sri Ernawati Srifariyati, Srifariyati Sudirman Yahya Sugeng Heri Suseno Sugiono Moeljopawiro Suharsono Suharsono Suharsono Suharsono Suharsono Suharsono Suharsono, Suharyadi Suharyadi Sulistiani, Erina Supena, Ence Darmo Jaya Sutiarso Sutiarso Suwaji Handaru Wardoyo Suwarno Suwarno Suwarno Suwarno TATIK CHIKMAWATI Tnunay, Ite Morina Yostianti Toto Hadiarto Trikoesoemaningtyas Turhadi Turhadi Utut Suharsono Utut Widyastuti Violita Violita Wardhani, Hilda Sari Wening Enggarini Windarti Wahyuningtyas Windriyana, Windriyana Yuriyah, S Yusrian, Afif Ramdhan Yusuf, Adib Fakhruddin Yuyun Rohmatul Uyuni Zurtiana, Ahmad