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AGROBACTERIUM-MEDIATED GENETIC TRANSFORMATION OF SEAWEED Kappaphycus alvarezii USING Gα GENE AND CALLUS CULTURES Erina Sulistiani; Suharsono Suharsono; Ence Darmo Jaya Supena; Miftahudin Miftahudin
BIOTROPIA Vol. 26 No. 1 (2019): BIOTROPIA Vol. 26 No. 1 April 2019
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.176 KB) | DOI: 10.11598/btb.2019.26.1.989

Abstract

Cottonii seaweed (Kappaphycus alvarezii Doty) is one of the most important commercial sources of carrageenan, which is widely used in the pharmaceutical and food industries. A major problem in the cultivation of this seaweed is ice-ice disease, which is caused by extreme changes in environmental conditions such as temperature and seawater salinity. Gene transformation to produce transgenic Kappaphycus lines that are tolerant to environmental stress is a potential solution to this problem. The Gα gene, which encodes the heterotrimeric G-protein alpha subunit, plays an important role in tolerance to biotic and abiotic environmental stress. This study aimed to: (a) introduce the Gα gene into the callus cells of K. alvarezii and regenerate transformed callus cells into transgenic plantlets; and (b) determine the appropriate concentration of acetosyringone and Agrobacterium tumefaciens strain for successful gene transfer into the callus of K. alvarezii. The callus cells of K. alvarezii were transformed using Agrobacterium tumefaciens strains LBA4404 and EHA105 carrying the expression vector pGWB502-Gα under the control of the CaMV-35S promoter. The calli and A. tumefaciens were co-cultivated in different concentrations of acetosyringone (20, 40, and 60 mg/L). The regeneration of transformed callus cells into transgenic plantlets was successfully achieved using the somatic embryogenesis technique. The results showed that the highest percentage of putative transgenic micropropagule formation occurred at acetosyringone concentrations of 20–40 mg/L. Polymerase chain reaction (PCR) analysis of twenty regenerated plantlets indicated that the Gα gene was successfully introduced into the genomic DNA of all samples. The highest transformation efficiency was obtained from the 20–40 mg/L acetosyringone co-cultivation treatment (22–28%). The transformation efficiency produced by Agrobacterium tumefaciens EHA105 (23%) was not significantly different from that produced using strain LBA4404 (15%).
PENDIDIKAN AKHLAK BERBASIS ALAM BAGI SISWA AUTISME MELALUI PENERAPAN PRINSIP PERILAKU TERAPAN Haikal Firdaus, Ahmad; Khadijah, Siti; Miftahudin, Miftahudin; Iksan, Iksan
Pendas : Jurnal Ilmiah Pendidikan Dasar Vol. 11 No. 03 (2026): Volume 11 Nomor 03, September 2026 Completed
Publisher : Program Studi Pendidikan Guru Sekolah Dasar FKIP Universitas Pasundan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23969/jp.v11i03.62463

Abstract

This research aims to explore the effectiveness of nature-based moral education in enhancing the understanding and practice of morals among autistic students through the application of Applied Behavior Analysis (ABA) principles. Moral education is crucial for autistic students to foster better social interactions and adaptive moral comprehension. This study employed a qualitative approach utilizing a case study method, involving participatory observation, in-depth interviews with educators and parents, and relevant document analysis. The research subjects were autistic students in an inclusive educational institution that had implemented a nature-based moral education program. Key findings indicate that the natural environment, rich in sensory and interactive stimuli, significantly facilitated the learning of moral concepts such as honesty, patience, and empathy. The application of ABA principles, including positive reinforcement and gradual behavior shaping, proved effective in guiding autistic students to internalize and express desired moral behaviors within natural contexts. The conclusion of this study affirms that nature-based moral education combined with ABA principles is a promising strategy for character development in autistic students, promoting their independence, and improving their quality of life
Pengaruh Kebijakan Dividen, Leverage, dan Earning Volatility terhadap Volatilitas Harga Saham pada Perusahaan Energi Sub Sektor Minyak dan Gas yang Terdaftar pada Bursa Efek Indonesia Periode 2021 - 2025 Miftahudin Miftahudin; Luthfi Nuraini; Umi Kulsum
Jurnal Rimba Riset Ilmu manajemen Bisnis dan Akuntansi Vol. 4 No. 3 (2026): Agustus : Riset Ilmu Manajemen Bisnis dan Akuntansi
Publisher : Asosiasi Riset Ilmu Manajemen Kewirausahaan dan Bisnis Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/rimba.v4i3.2762

Abstract

This study aims to examine the effect of dividend policy, leverage, and earnings volatility on stock price volatility in oil and gas sub-sector energy companies listed on the Indonesia Stock Exchange (IDX) during the 2021–2025 period. This research employs a quantitative approach using secondary data obtained from the companies' financial statements and stock price data. The sample was selected using a purposive sampling technique, resulting in 12 companies that met the predetermined criteria over a five-year observation period. The data were analyzed using panel data regression with the assistance of EViews 13 software. Based on the results of the Chow test and Hausman test, the Fixed Effect Model (FEM) was selected as the most appropriate model. The findings indicate that, partially, dividend policy as measured by the Dividend Payout Ratio (DPR) has a negative and significant effect on stock price volatility. Meanwhile, leverage, as measured by the Debt-to-Equity Ratio (DER), has no significant effect on stock price volatility. Likewise, earnings volatility has no significant effect on stock price volatility. Simultaneously, dividend policy, leverage, and earnings volatility have a significant effect on stock price volatility. These findings suggest that dividend distribution policy is the primary factor considered by investors in responding to stock price movements of oil and gas sub-sector energy companies, while leverage and earnings volatility do not individually have a significant influence on stock price volatility.
Perencanaan Strategis Sistem Informasi Pondok Pesantren Daarul Mizan Menggunakan Metode Ward Peppard Windriyana, Windriyana; Miftahudin, Miftahudin; Hilmi Nugraha, Mochammad
Jutisi : Jurnal Ilmiah Teknik Informatika dan Sistem Informasi Vol. 15 No. 4 (2026): Agustus 2026
Publisher : STMIK Banjarbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35889/jutisi.v15i4.3714

Abstract

The rapid flow of information technology is currently forcing the education sector to rapidly undertake digital transformation for the sake of governance efficiency and improved service quality. This phenomenon also applies to Islamic boarding schools (pesantren), which are now being encouraged to adopt integrated information systems to align their academic and administrative activities. Unfortunately, Daarul Mizan Islamic Boarding School still struggles with classic challenges: fragmented data and manual work mechanisms that have not yet been touched by digitalization. Without a clear IS/IT strategic direction, the institution's operations are slow and policy-making is inaccurate. Therefore, this study focuses on the development of IS/IT strategic planning within Daarul Mizan Islamic Boarding School, using the Ward and Peppard framework. The evaluation process was comprehensive, starting with analyzing internal and external conditions through SWOT and value chain analysis, and mapping future applications using the McFarlan Strategic Grid. The output of this study includes a roadmap and recommended application portfolios that are expected to catalyze Daarul Mizan's digital transformation. Keywords: IS Strategy; Ward and Peppard Model; Islamic Boarding School Digitalization; Information Governance. Abstrak Pesatnya arus teknologi informasi saat ini memaksa sektor pendidikan untuk segera melakukan transformasi digital demi efisiensi tata kelola dan mutu layanan yang lebih baik. Fenomena ini juga berlaku bagi pondok pesantren yang kini didorong untuk mengadopsi sistem informasi terpadu guna menyelaraskan aktivitas akademik dan administrasinya. Sayangnya, Pondok Pesantren Daarul Mizan masih bergelut dengan tantangan klasik: data yang terfragmentasi serta mekanisme kerja manual yang belum tersentuh digitalisasi. Tanpa arah strategis SI/TI yang jelas, operasional lembaga menjadi lambat dan pengambilan kebijakan pun kurang akurat. Oleh karena itu, studi ini memfokuskan pada penyusunan perencanaan strategis SI/TI di lingkungan Pondok Pesantren Daarul Mizan dengan mengacu pada kerangka kerja Ward and Peppard. Proses evaluasi dilakukan secara menyeluruh, mulai dari membedah kondisi internal-eksternal melalui analisis SWOT dan value chain, hingga memetakan aplikasi masa depan menggunakan McFarlan Strategic Grid. Luaran dari penelitian ini mencakup peta jalan (roadmap) serta rekomendasi portofolio aplikasi yang diharapkan mampu menjadi katalisator bagi transformasi digital di Daarul Mizan. Kata Kunci: Strategi SI; Model Ward and Peppard; Digitalisasi Pesantren; Tata Kelola Informasi.
Differential Gene Expression of Ethylene Biosynthesis and Signaling Associated with Climacteric Behaviors in Indonesian Melon Latifah, Vida Rahma; Yusuf, Adib Fakhruddin; Putro, Karso Suryo; Sartika, Dian; Wibowo, Anjar Tri; Nurhayati, Awik Puji Dyah; Meitha, Karlia; Miftahudin, Miftahudin; Daryono, Budi Setiadi
Makara Journal of Science Vol. 30, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Fruit ripening in melon (Cucumis melo L.) is a complex developmental process that is characterized by the coexistence of climacteric and non-climacteric varieties within the same species. This study characterized the ripening behavior of four local Indonesian melon cultivars (“Melona”, “GMP”, “Tacapa Gold”, and “Kinaya”) by integrating morphophysio-logical traits with transcriptional expression analysis of ethylene biosynthesis (CmACS, CmACO) and signaling (CmATH, CmEREBP) genes. The results revealed a strong agreement between the phenotypic traits and molecular regulation. “Melona” and “GMP” exhibited a strong climacteric profile, which was characterized by natural fruit abscission, sweet aroma production, and dramatic upregulation of CmACS. Conversely, “Tacapa Gold” aligned with a non-climacteric pro-file, exhibiting neither abscission nor aroma. Notably, “Kinaya” displayed a unique intermediate phenotype, combining a transient peak in CmACO expression and an enhanced softening rate with the absence of typical ethylene-dependent traits. Flesh softening and sugar accumulation progressed across all cultivars, thus confirming the presence of ethylene-independent pathways. Classifying these cultivars based on their specific position along the ripening continuum presents a robust framework for optimizing targeted harvest timing and tailored postharvest management strategies
Co-Authors AD Ambarwati ADEEL ABDUL KARIM ALTUHAISH Afifah Azkiyah Ahmad Dwi Setyawan Albarokah, Nurdiansyah ALEX HARTANA ALEX HARTANA Amarullah, Mohammad Syarifuddin Ambarwati, AD Anarki, Aprillia Alling Aris Tjahjoleksono Atang Sutandi Awik Puji Dyah Nurhayati Aziz, Alvi BUDI SETIADI DARYONO CHAIDIR CHAIDIR Darmawan, Gilang Dasumiati Dasumiati Desvian Bandarsyah Devi Media Siska Dewi Indriyani Roslim DIANA AGUSTIN CAROLINA DIBYO PRONOWO Dwi Suci Setyaningsih DWINITA WIKAN UTAMI Dwinita Wikan Utami Eka Setiawan Erina Sulistiani Faiz, Agus Maulana Fauzobihi, Fauzobihi Fuad Fitriawan, Fuad Habizar, Habizar Hafizhni Anggia, Rafi Haikal Firdaus, Ahmad HAJRIAL ASWIDINNOOR Hamim Hamim Hanarida, I Harisca, Rodhi Hartanti, Fajri Hartati, N Sri Hilmi Nugraha, Mochammad HIMMAH RUSTIAMI I Hanarida I PUTU GEDE P. DAMAYANTO I Rosdianti Iksan, Iksan Imamudin Imamudin Iman Rusmana Iswandi Anas Isya, Mandanu Akhta Nur J PERRY GUSTAFSON Joko Prasetiyono JULIARNI JULIARNI Juliyani Juliyani Kamini, Kamini Khoirurrijal Khoirurrijal, Khoirurrijal Komalasari, Selly Siti Komalasari Kustiariyah, Kustiariyah Kusumanegara, Kusumawaty LA KOLAKA Latifah, Vida Rahma Luthfi Nuraini M Ilham Fahmi Roji Machdum Bachtiar MARIA BINTANG Marlina Ardiyani Meitha, Karlia Moeljopawiro, Sugiono Muchozin, Muhammad Ni'an Muhammad Akbar Velayati Muhammad Alfarabi Mukhlash Abrar Munif Ghulamahdi Muslimin Muslimin MUTTAQIN, MAFRIKHUL Nasir, Fahad Muhammad Nasir Nettyani, Naipospos Nugroho, Giri Nunung Fajaryani, Nunung Nurlaela Nurlaela Nurlaela Nurlaela Nurlaila, Santi Nurlaila OKKY SETYAWATI DHARMAPUTRA Pieter Agusthinus Riupassa Polosoro, Aqwin Prasetiyono, Joko Purnama Arafah Purwanti, Tira Puspita Dewi Sopyati Putri Aulia Putro, Karso Suryo RANTJE LILLY WORANG Rasyid, Aliy Ratnasari, Seli Risa Aryantri Rizal Nursamsi, Dede RIZAL SYARIEF Robby Maududy Rohimah, Rt Bai Rosdianti, I S Yuriyah Saepudin Saepudin Sahab, Fahmi Sahab Sairun Sairun Salimah, Hafshah Aqidatun Sandra Arifin Aziz Sartika, Dian Setiawan Setiawan Setiawan, Jeffry SISWA SETYAHADI Siti Khadijah Siti Maryam Harahap Slamet, Oky Sobir Sobir Sokoy, Mercy Sopandi, Nur Irfan Srifariyati, Srifariyati Sudirman Yahya Sugeng Heri Suseno Sugiono Moeljopawiro Suharsono Suharsono Suharsono Suharsono Suharsono, Suharyadi Suharyadi Supena, Ence Darmo Jaya Sutiarso Sutiarso Suwaji Handaru Wardoyo Suwarno Suwarno Suwarno Suwarno Tabrani, M. Bayi TATIK CHIKMAWATI Tnunay, Ite Morina Yostianti Toto Hadiarto Tri Anjar Triadiati Triadiati Trikoesoemaningtyas Turhadi Turhadi Umi Kulsum Utut Suharsono Utut Widyastuti Violita Violita Wardhani, Hilda Sari Wening Enggarini Windarti Wahyuningtyas Windriyana, Windriyana Yuriyah, S Yusrian, Afif Ramdhan Yusuf, Adib Fakhruddin Yuyun Rohmatul Uyuni Zurtiana, Ahmad