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All Journal HAYATI Journal of Biosciences Vegetalika Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Bios Logos Jurnal Natur Indonesia Jurnal Forum Nuklir Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Jurnal Hortikultura Jurnal AgroBiogen Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Sumberdaya Hayati BERITA BIOLOGI Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biodjati Jurnal Penelitian Pertanian Tanaman Pangan Biosaintifika: Journal of Biology & Biology Education JURNAL PENDIDIKAN TAMBUSAI Jurnal Ilmiah Mandala Education (JIME) Jurnal Ilmu Kefarmasian Indonesia Amalee: Indonesian Journal of Community Research & Engagement Aphorisme: Journal of Arabic Language, Literature, and Education Informatics and Digital Expert (INDEX) NUSRA: Jurnal Penelitian dan Ilmu Pendidikan Jurnal Minfo Polgan (JMP) Indonesian Journal of EFL and Linguistics Jurnal Pusat Inovasi Masyarakat Formosa Journal of Science and Technology (FJST) Makara Journal of Science Eduvest - Journal of Universal Studies AL QALAM JURNAL KEPENDIDIKAN SINTESA Jurnal Intelek Insan Cendikia Reinwardtia Indonesian Journal of Innovation Multidisipliner Research Jurnal Ekonomi, Manajemen, Akuntansi Jurnal Pendidikan dan Sosial Humaniora Istifkar: Media Transformasi Pendidikan

Miftahudin .

Department Of Biology, Faculty Of Mathematics And Natural Sciences, Bogor Agricultural University, Bogor

Author-ID : 320114

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Rye (Secale cereale L.) and Wheat (Triticum aestivum L.) Simple Sequence Repeat Variation within Secale spp. (Poaceae) TATIK CHIKMAWATI; . MIFTAHUDIN; J PERRY GUSTAFSON
HAYATI Journal of Biosciences Vol. 20 No. 4 (2013): December 2013
Publisher : Bogor Agricultural University, Indonesia

Transferability of 25 rye and 20 wheat simple sequence repeat (SSR) markers were examined across Secale spp. to elucidate their genetic relationships. One hundred percent of rye and 75% of wheat SSR markers could be amplified in weedy and wild forms of Secale. However, only 84% of rye SSR markers were amplified in S. sylvestre indicating that this species had the furthest relation to the cultivated rye. The PCR products resulting from rye SSR tended to have similar size as the original size, while wheat SSRs tended to have a shorter size than that of the original size. Polymorphism in the allelic number and size was found in 68% of the rye and 40% of the wheat SSR markers. Several alleles were unique for the annual and perennial rye taxa, which were counted for 22 and 15%, respectively. Some SSR markers also produced specific alleles that could be used to identify certain taxa. The genetic relationships within the genus Secale based on SSR markers were discussed.

Field Adaptation of Some Introduced Wheat (Triticum aestivum L.) Genotypes in Two Altitudes of Tropical Agro-Ecosystem Environment of Indonesia ADEEL ABDUL KARIM ALTUHAISH; . MIFTAHUDIN; . TRIKOESOEMANINGTYAS; SUDIRMAN YAHYA
HAYATI Journal of Biosciences Vol. 21 No. 1 (2014): March 2014
Publisher : Bogor Agricultural University, Indonesia

Heat stress is a major environmental factor limiting wheat productivity in tropical regions such as Indonesia. The objective of this study was to investigate the adaptability of introduced wheat genotypes in tropical agro-ecosystems. Sixteen spring wheat genotypes were grown at two different altitudes i.e. low altitude (176 m asl) with an average temperature of 29.8 oC located at Leuwikopo Field Experimental Station, Bogor Agricultural University, Darmaga Bogor and high altitude (1100 m asl) with an average temperature of 20.6 oC at Cipanas Field Experimental Station (Ornamental Crop Research Station), Cianjur, West Java, Indonesia from July to November 2012. Plant height, number of tillers, flag leaf area, leaf angle, days to flowering, spike number per plant, empty spikelet number, grain weight per plant and 100 grain weight were observed following the standard methods. Heat susceptibility index was calculated based on grain weight per plant. The results showed that cultivation at a low altitude, hotter environment remarkably affected wheat growth and yield, as reflected in overall reduction of plant height, reduced number of tillers and leaf area, and ultimately reduced yield and yield components for most genotypes compared to the same measures taken at high altitude in lower temperatures. Plant growth before heading was similar in both locations, but the days to flowering was longer in high altitude than that in low altitude. High temperature stress in low altitude reduced the spike number/plant, grain weight/plant, 100 grain weight and increase number of empty spikelet/spike. Based on our results for heat susceptibility index, six genotypes, namely Sbr, Ymh, Astreb/Cbrd, Astreb/Ningma, H-20 and Nias, were characterized as heat tolerant genotypes.

Increasing Hermaphrodite Flowers using Plant Growth Regulators in Andromonoecious Jatropha curcas . DASUMIATI; . MIFTAHUDIN; . TRIADIATI; ALEX HARTANA; DIBYO PRONOWO
HAYATI Journal of Biosciences Vol. 21 No. 3 (2014): September 2014
Publisher : Bogor Agricultural University, Indonesia

Jatropha curcas (JC) is a crop with potential for use in biodiesel. Production of biodiesel requires plant seed as raw material, so the viability of JC for use in biodiesel will dependent greatly on the plant’s production of flowers. Generally, this plant is monoecious, meaning it has both male and female flowers. However, very rarely JC plants may be andromonoecious. Andromonoecious specimens of JC produce hermaphrodite and male flowers in the same plant. The number of hermaphrodite flowers per inflorescence is generally low compared to the number of male flowers. The aim of this study was to increase the proportion of hermaphrodite flowers by using plant growth regulators (PGRs) in andromonoecious JC. Our experiment was conducted in Randomized Block Design (RBD) with 9 treatments, namely kinetin, GA3, and IAA with concentrations of 0 ppm as a control, 50 and 100 ppm of each PGRs. The treatments were applied to stem cuttings from each plant and repeated 4 times. PGRs were applied by spraying the leaves within the buds of each plant. Applications took place weekly beginning when the plants entered flower initiating phase, until inflorescence produced. Observations were conducted during the treatment period (10 weeks). Results showed that plants treated with IAA, GA3, and kinetin at 50 and 100 ppm produced increased inflorescence per plant. The increases measured were 155.4 and 92.9% of (IAA), 120.4 and 151% (GA3), 96.6 and 51.7% (kinetin) respectively. In addition we found that application and GA3 at concentrations of 50 and 100 ppm, and kinetin at 50 ppm, increased the number of hermaphrodite flowers per inflorescence by 50%, and increased the number of hermaphrodite flowers per plant by 275.6 and 183.1% (IAA), 219.5 and 254.1% (GA3), 162.9 and 103.1% (kinetin) respectively. As would be expected, the number of fruit per plant increased in those specimens treated with IAA, GA3, and kinetin at 50 and 100 ppm. The increases measured were 301.7 and 167.4% (IAA), 211.7 and 257.0% (GA3), 162.5 and 101.4% (kinetin) respectively.

Fine Root Production and Decomposition in Lowland Rainforest and Oil Palm Plantations in Sumatra, Indonesia Violita Violita; Triadiati Triadiati; Iswandi Anas; Miftahudin Miftahudin
HAYATI Journal of Biosciences Vol. 23 No. 1 (2016): January 2016
Publisher : Bogor Agricultural University, Indonesia

Transformation of tropical rainforest into oil palm plantation not only has impacts on biodiversity but also affects ecosystem functions such as production and decomposition of fine roots as a nutrient source for plant. The objective of the research was to evaluate the production and decomposition rate of fine roots in natural forest (NF) at Bukit 12 National Park and oil palm plantation (OP) in Jambi, Sumatra. The soil core and litter bag methods were used to obtain fine root production and decomposition data. The results showed that generally, there was the same pattern in fine root production between NF and OP. The annual fine root productivity was found to be higher in NF than that of OP. Rainfall in NF and air temperature in NF and OP were the most significant climate factors affecting fine root production. The remaining fine root biomass decreased as the incubation time increased. The decomposition rate constant (k value) was significantly higher in NF than in OP. Our data showed that the nutrient turn-over of NF fine roots was faster than of OP fine roots. Nitrogen, carbon content, and C/N ratio were the main factors that influenced fine root decomposition.

Construction of RNA Interference Vector to Silence Aluminum Tolerance Gene Candidate in Rice cv Hawara Bunar Windarti Wahyuningtyas; Miftahudin Miftahudin; Utut Widyastuti; Aris Tjahjoleksono
HAYATI Journal of Biosciences Vol. 23 No. 2 (2016): April 2016
Publisher : Bogor Agricultural University, Indonesia

One of the aluminum (Al) tolerance gene candidates, namely B11 gene, has been successfully isolated from Al-tolerant rice cv Hawara Bunar. However, the role of the gene in Al tolerance in rice has not been known. RNA interference (RNAi) technique is an effective tool to examine the biological function of the target gene in plant. The objective of the research was to construct RNAi recombinant vector carrying untranslated region of the B11 gene. RNAi recombinant vector carrying 195 bp sized 3′UTR_B11 fragment as a double-stranded RNA (dsRNA) trigger has been successfully constructed using GATEWAY™ cloning technology, pENTR™/D-TOPO® as a shuttle vector, and pANDA vector as a destination vector. RNAi construct was successfully introduced into Agrobacterium tumefaciens AgL0, and has been infected to rice cv Hawara Bunar. Analysis of putative transgenic rice showed eight of 20 plants were transgenic carrying the B11-RNAi construct.

Overexpression of B11 Gene in Transgenic Rice Increased Tolerance to Aluminum Stress Devi Media Siska; . Hamim; . Miftahudin
HAYATI Journal of Biosciences Vol. 24 No. 2 (2017): April 2017
Publisher : Bogor Agricultural University, Indonesia

Rice cultivation on acid soils is mainly constrained by aluminum (Al) toxicity. However, rice has tolerance mechanism to Al stress, which is controlled by many genes. B11 gene is one of the Al- tolerance gene candidate isolated from rice var. Hawara Bunar. It has not been known whether overexpression of the gene in Al-sensitive rice is able to increase Al tolerance. The research objective was to analyze root morphological and physiological responses of transgenic rice overexpressing B11 gene to Al stress. The experiment was carried out using five rice genotypes including two varieties (Hawara Bunar and IR64) and three T4 generation of transgenic lines, that are T8-2-4, T8-12-5, and T8-15-41. All rice genotypes were grown in nutrient solution for 24 h (adaptation period), and then were exposed to 15 ppm Al for 72 h (treatment period) and recovered in normal nutrient solution for 48 h (recovery period). The result showed that the overexpression of the B11 gene in T8-2-4, T8-12-5, and T8-15-41 transgenic lines improved tolerance to Al stress based on root growth characters, accumulation of Al, root cell membrane lipid peroxidation, and root tip cell structure.

Pengembangan Marka Molekuler yang Berasosiasi Dengan Kekuatan Dinding Sel Penyusun Saluran Getah Kuning Pada Manggis Risa Aryantri; Miftahudin Miftahudin; Sobir Sobir
Jurnal Hortikultura Vol 24, No 1 (2014): Maret 2014
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v24n1.2014.p16-22

Salah satu masalah utama dalam peningkatan kualitas buah manggis ialah pencemaran buah oleh getah kuning akibat pecahnya sel penyusun saluran getah kuning. Seleksi buah manggis untuk mendapatkan buah bebas getah kuning dapat dibantu dengan memanfaatkan marka molekuler terpaut karakter kekuatan dinding sel penyusun saluran getah kuning. Penelitian ini dilaksanakan di Laboratorium Pusat Kajian Hortikultura Tropik (PKHT), Institut Pertanian Bogor Jawa Barat, pada Bulan Mei 2012 sampai dengan April 2013. Penelitian ini bertujuan mengembangkan marka molekuler yang berasosiasi dengan sifat kekuatan dinding sel penyusun saluran getah kuning pada manggis. Sebanyak 39 aksesi Garcinia mangostana L. koleksi Balai Penelitian Tanaman Buah Tropika, Sumatera Barat dan hasil koleksi dari Desa Leuwiliang, Jawa Barat digunakan dalam penelitian ini. Dua pasang primerdikembangkan dari sekuen gen kekuatan dinding sel manggis dengan teknik tersarang (nested PCR). Amplifikasi DNA dilakukan dengan teknik PCR standar. Hasil amplifikasi DNA menunjukkan satu pita DNA berukuran ± 260 pb yang polimorf antara tanaman dengan buah tercemar getah kuning dan tidak tercemar getah kuning. Pita tersebut berasosiasi dengan sifat kekuatan dinding sel penyusun saluran getah kuning.

PENGGUNAAN TEKNIK RADIOGRAFI NEUTRON UNTUK MENDETEKSI SECARA CEPAT TOLERANSI TANAMAN PADI TERHADAP CEKAMAN KEKERINGAN Sutiarso Sutiarso; Miftahudin Miftahudin; Sairun Sairun; Juliyani Juliyani; Setiawan Setiawan; Fahrurrozi Fahrurrozi
Jurnal Forum Nuklir JFN Vol 6 No 2 November 2012
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17146/jfn.2012.6.2.3416

PENGGUNAAN TEKNIK RADIOGRAFI NEUTRON UNTUK MENDETEKSI SECARA CEPAT TOLERANSI TANAMAN PADI TERHADAP CEKAMAN KEKERINGAN. Telah dilakukan penggunaan teknik radiografi neutron untuk mendeteksi secara cepat toleransi tanaman padi terhadap cekaman kekeringan. Dua jenis padi yaitu padi gogo dan padi sawah dipilih sebagai obyek penelitian. Tanaman padi gogo yang diketahui lebih toleran terhadap cekaman kekeringan dibanding padi sawah diamati perkembangan akarnya setelah mengalami cekaman kekeringan dengan mengurangi kandungan air tanahnya hingga 50% dan 25%. Pengamatan perkembangan akar tanaman diamati untuk usia tanaman 2, 3, 4, serta 10 minggu. Pengamatan dengan fasilitas radiografi neutron, RN1 di reaktor RSG-GAS secara in-situ menunjukkan bahwa untuk usia padi di bawah 4 minggu perkembangan akar tidak teramati dan akar mulai teramati dengan jelas pada usia tanaman 10 minggu. Hasil awal menunjukkan babwa padi gogo terbukti lebih toleran terhadap cekaman kekeringan dengan profil akar yang relatif lebih besar dan mampu bertahan lebih lama terhadap cekaman kekeringan dibanding dengan padi sawah. Hasil ini menunjukkan bahwa teknik radiografi neutron dapat menjadi metode alternatif untuk mendeteksi toleransi tanaman padi terhadap cekaman kekeringan.

Pemberdayaan Masyarakat pada Budi Daya dan Pengembangan Produk Pohon Gaharu (Aquilaria sp.) di Kabupaten Tolitoli, Sulawesi Tengah Triadiati Triadiati; Miftahudin Miftahudin
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 7 No. 2 (2021): Agrokreatif Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.7.2.174-184

Tolitoli District has a potential for agarwood trees in the forests, but it has not been optimally utilized and preserved by the local communities. Therefore, efforts are needed to increase understanding and community involvement in cultivation and product development of agarwood trees. The objectives of this activity are: to explore agarwood tree species in the forest and sources of local inoculums using a purposive sampling method for agarwood production, to assess the suitability of agro-climates for agarwood tree cultivation, to conduct face-to-face training and practice for agarwood tree breeding and bio-induction by injection, and to identify socio-economic conditions to support agarwood tree cultivation for community empowerment through product development and cultivation in Kabupaten Tolitoli. The project was implementated by exploring and identifying the existence of natural agarwood trees and agarwood farmers, training, and mentoring. The results of exploration and identification showed that Kabupaten Tolitoli has natural resources of agarwood trees in the forest and local inoculums for the bio-induction of agarwood formation. Also, the local community, including the local government, practitioners, farmers, and educational institutions, have great interest and desire for product development and cultivation of agarwood trees. Thus, it can be concluded that community empowerment through product development and cultivation of agarwood trees in Kabupaten Tolitoli can be implemented.

Pertumbuhan dan Kandungan Bahan Bioaktif Selaginella plana dan Selaginellla willdenovii pada Beberapa Media Tanam Miftahudin .; Dwi Suci Setyaningsih; Tatik Chikmawati
Jurnal Sumberdaya Hayati Vol. 1 No. 1 (2015)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.1.1.1-6

Selaginella, a genus of Pteridophyte, is known as an herb that contains of several bioactive compounds. The growth and bioactive compounds of plant were affected by plant environment, such as media types. The objective of the research was to obtain an appropriate plant media for growing S. plana dan S. willdenovii, and its effect on the content of bioactive compounds. The experiment consisted of two factors, plant growth media and the species of Selaginella. The first factor consisted of eight plant growth media, i.e.: soil, rice husk, rice husk : soil = 1:1 (v/v), burned rice husk, soil : burned rice huks = 1:1 (v/v), burned rice husk : rice husk = 1:1 (v/v), burned rice husk : rice husk = 3:1 (v/v), and burned rice husk : rice husk = 1:3 (v/v). The second factor was two spesies of Selaginella, i.e.: S. plana and S. willdenovii. Ethanol extract of Selaginella was qualitatively analyzed for flavonoid, tannin, and saponin content. The result showed that both fresh and dry weights of Selaginella were influenced by the type of plant media, Selaginella species, and their interactions. S. plana and S. willdenovii grew best on medium containing soil: burned rice = 1:1 (v/v). The highest flavonoid content of S. plana and S. willdenovii was also obtained from the plant grown on the same media. Comparing to the bioactive compound content of natural plant, saponin content of S. plana and S. willdenovii was increased by the treatment of plant media. The treatment also increased tannin content of S. plana, but not of S. willdenovii.

Co-Authors , Triadiati AD Ambarwati ADEEL ABDUL KARIM ALTUHAISH Afifah Azkiyah Ahmad Dwi Setyawan Ahmad Tarmidi Albarokah, Nurdiansyah ALEX HARTANA ALEX HARTANA Amarullah, Mohammad Syarifuddin Ambarwati, AD Anarki, Aprillia Alling Anjar Tri Wibowo Arafah, Purnama Aris Tjahjoleksono Atang Sutandi Awik Puji Dyah Nurhayati Aziz, Alvi Bachtiar, Machdum BUDI SETIADI DARYONO Budiarto, Despiyan Dwi CHAIDIR CHAIDIR Dasumiati Dasumiati Desvian Bandarsyah Devi Media Siska Dewi Indriyani Roslim DIANA AGUSTIN CAROLINA DIBYO PRONOWO Dwi Jatmoko Dwi Suci Setyaningsih Dwinita Wikan Utami DWINITA WIKAN UTAMI Eka Setiawan Fahrurrozi Fahrurrozi Faiz, Agus Maulana Fauzobihi, Fauzobihi Fuad Fitriawan, Fuad Habizar, Habizar Hafizhni Anggia, Rafi HAJRIAL ASWIDINNOOR Hamim Hamim Hanarida, I Harisca, Rodhi Hartanti, Fajri Hartati, N Sri Haryanti, Intisari HIMMAH RUSTIAMI Himmah Rustiami, Himmah I Hanarida I PUTU GEDE P. DAMAYANTO I PUTU GEDE P. DAMAYANTO, I PUTU GEDE P. I Rosdianti Ilham Dinar Akbar Cudino Iman Rusmana Iswandi Anas Isya, Mandanu Akhta Nur Iwan, Pristanto Ria Irawan J PERRY GUSTAFSON Joko Prasetiyono JULIARNI JULIARNI Juliyani Juliyani Kamini, Kamini Khoirurrijal Khoirurrijal, Khoirurrijal Komalasari, Selly Siti Komalasari Kustiariyah, Kustiariyah Kusumanegara, Kusumawaty LA KOLAKA Latifah, Vida Rahma M Ilham Fahmi Roji MARIA BINTANG Marlina Ardiyani Maududy, Robby Meitha, Karlia Moeljopawiro, Sugiono Muchozin, Muhammad Ni'an Muhammad Akbar Velayati Muhammad Alfarabi Mukhlash Abrar Munif Ghulamahdi Muslimin Muslimin MUTTAQIN, MAFRIKHUL Nadzar Saifudin Nasir, Fahad Muhammad Nasir Nettyani, Naipospos Nugroho, Giri Nunung Fajaryani, Nunung Nurlaela Nurlaela Nurlaela Nurlaela Nurlaila, Santi Nurlaila OKKY SETYAWATI DHARMAPUTRA Pieter Agusthinus Riupassa Polosoro, Aqwin Prasetiyono, Joko Purwanti, Tira Puspita Dewi Sopyati Putri Aulia Putro, Karso Suryo Raden Mohamad Herdian Bhakti RANTJE LILLY WORANG Rasyid, Aliy Ratnasari, Seli Risa Aryantri Rizal Nursamsi, Dede RIZAL SYARIEF Rosandi Ardi Noegraha Rosdianti, I Ruwirohi, Jan Everhard S Yuriyah Sahab, Fahmi Sahab Sairun Sairun Salimah, Hafshah Aqidatun Sandra Arifin Aziz Sartika, Dian Setiawan Setiawan Setiawan, Jeffry SISWA SETYAHADI Siti Maryam Harahap Slamet, Oky Sobir Sobir Sokoy, Mercy Sopandi, Nur Irfan Sri Ernawati Srifariyati, Srifariyati Sudirman Yahya Sugeng Heri Suseno Sugiono Moeljopawiro Suharsono Suharsono Suharsono Suharsono Suharsono Suharsono Suharsono, Suharyadi Suharyadi Sulistiani, Erina Supena, Ence Darmo Jaya Sutiarso Sutiarso Suwaji Handaru Wardoyo Suwarno Suwarno Suwarno Suwarno TATIK CHIKMAWATI Tnunay, Ite Morina Yostianti Toto Hadiarto Trikoesoemaningtyas Turhadi Turhadi Utut Suharsono Utut Widyastuti Violita Violita Wardhani, Hilda Sari Wening Enggarini Windarti Wahyuningtyas Windriyana, Windriyana Yuriyah, S Yusrian, Afif Ramdhan Yusuf, Adib Fakhruddin Yuyun Rohmatul Uyuni Zurtiana, Ahmad

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