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All Journal JURNAL KIMIA SAINS DAN APLIKASI Alchemy : Journal of Chemistry Tadris: Jurnal keguruan dan Ilmu Tarbiyah Jurnal technoscientia Al Fikra : Jurnal Ilmiah Keislaman Indonesian Journal of Chemistry JURNAL PENDIDIKAN TAMBUSAI Cakrawala : Jurnal Kajian Manajemen Pendidikan Islam dan Studi Sosial Instructional Development Journal JURNAL TEKNOLOGI TECHNOSCIENTIA Jurnal Pusat Studi Pendidikan Rakyat (PUSDIKRA) NUSRA: Jurnal Penelitian dan Ilmu Pendidikan Al-MIKRAJ: Jurnal studi Islam dan Humaniora Makara Journal of Science Molekul: Jurnal Ilmiah Kimia Najah: Journal of Research and Community @-Publik: Jurnal Administrasi Publik Bulletin of Chemical Reaction Engineering & Catalysis Journal Mobile Technologies (JMS) International Journal of Universal Education Jurnal Ilmiah Pendidikan Citra Bakti
Mudasir Mudasir
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Universitas Gadjah Mada, Sekip Utara PO BOX BLS 21 Yogyakarta 55281
Religion Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Social Sciences Other

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Journal : Makara Journal of Science

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Systematic Review: Recent Developments in Magnetic-Nanoparticle-Based DNA Extraction Tjoa, Stanley Evander Emeltan; Mudasir, Mudasir; Suharyadi, Edi; Daryono, Budi Setiadi
Makara Journal of Science Vol. 29, No. 2
Publisher : UI Scholars Hub

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Abstract

Deoxyribonucleic acid (DNA) is a research object for various molecular fields of study, and DNA extraction is a basic procedure in molecular biology laboratories. DNA extraction methods are evolving, from several steps using organic solvents to recent technology using magnetic nanoparticles as adsorbents for the purification step. The use of magnetic nanoparticles for DNA extraction is magnetizing because it is simple without heavy machinery, such as centrifugation and organic solvents. The application of this method is extensive and diverse. This systematic review summarizes current development trends for some aspects of this DNA extraction system from the magnetic nanoparticle synthesis method, extraction protocol, and different types of samples used. The main purpose of this review is to provide an overall perspective of the use of magnetic nanoparticles in DNA extraction for researchers to start their exploration of this safe, simple, and rapid technique. In general, the most well-known magnetic nanoparticle preparation uses the coprecipitation method. The preferred particle is a plain and silica-coated magnetic nanoparticle using tetraethyl orthosilicate. The most common buffer systems for binding, washing, and elution are Polyethylene Glycol (PEG) and NaCl, 70% ethanol, and Tris–EDTA, respectively. The extraction system with magnetite can be used for various types of samples, ranging from human specimens to animal, plant, and bacterial specimens. The extraction yields using the magnetic-nanoparticle-based method were between 0.03 µg and 116.63 µg with a DNA purity ratio between 1.09 and 1.99.
Co-Authors Adelin, Muhammad Ady Ferdian Noor Aflizah, Nur Agustiar agustiar ALGUSYAIRI, PARISYI Amyus, Randi Saputra Angglina, Novita Anjar Purba Asmara Astari, Siti Alysha Asteriniah, Femi As’ari, As’ari Bambang Rusdiarso BUDI SETIADI DARYONO Busra, Aminudin Dahrani, Dahrani Dalillah, Aupi Dari, Sri Wulan Darto Darto Desi Arisandi Dinata, Yuriyan Dwi Siswanta Edi Suharyadi Fadel, Moh. Fani, Tedy Dwi Fitriana, Dina Fitriyadi, Muhammad Habib Abdullah, Habib Hafis, Gustianto Nur Hamami, Abdul Rasyid Hartono Hartono Heryanto, Edi Hidayat, Rizki Icuk Muhammad Sakir Indrawan, Tiwa Junio Iriani, Atrika Itasaiyah, Itasaiyah Khairunnisa, Aulia Liyadi, Muslim Lubis, Nasib Samsuri Madaniyah, Madaniyah Mardhiyah, Mardhiyah Mardiah Mardiah Mardianto Mardianto Mat Syuroh Mawar Mawar, Mawar Muharriyadi, Muharriyadi Muhtady, Dedi Musdiani Musdiani Muti'ah, Siti Nangim, Ngainun Nuniek Herdyastuti Nurhafizah Nurhafizah Nurhasanah Nurhasanah Nurul Hidayat Aprilita Nurwijayanti Rahmawan, Arsuwandika Ramadhan, Muhammad Rizki Rizadiliyawati, Rizadiliyawati Rohadi Rohadi Rohaini, Yespi Romadhona, Miftakhul Romodhon, M Iqbal Roto Roto RR. Ella Evrita Hestiandari S, Rayani V Sabirin Matsjeh Sahrizal, M. Rinaldi Saifuddin Saifuddin Saputra, Aliyus Saputra, Ramli Saputri, Eki Nining Sari, Dwi Nofika Saswita, Saswita Septiani, Iga Shihabuddin, Ahmad Sibarani, Yetti Erika Sri Juari Santosa SRI RAHAYU Sri Sunarsih Suherman, Suherman Susiana, Carissa Ayu Sutinah Sutinah Syuhada Syuhada Thahir, Musa Titin Aryani Tjoa, Stanley Evander Emeltan Tri Gunawan, Tri Tri Joko Raharjo Utomo, Edi Yovianti, Ice Veronika Zainuddin, Abdul wahid Zaitun Zaitun Zofir, Tasrif Zuanda, Sary