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Formulation and Stability Test of HPMC and Carbomer 940 based-Gel Formulation Containing Plantago major L. hydroalcoholic extract Khafi, Muhammad; Hertiani, Triana; Murwanti, Retno; Bayu Irawan, Muhamad; Marzuki, Ahmad; Dwi Salim, Rozin
Majalah Farmaseutik Vol 21, No 2 (2025)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/farmaseutik.v21i2.95893

Abstract

The P. major plant has long been utilized in traditional herbal medicine and is widely accessible in Indonesia and globally. Extensive research has demonstrated its efficacy in wound healing through both clinical and preclinical studies. Gel formulations are recognized for establishing a conducive moist environment essential for wound healing, thus rendering them suitable for topical wound treatment. This study assesses the stability of HPMC gel base, carbomer 940, and their combination as carriers for the ethanol extract of P. major leaves. Stability testing involved a freeze-thaw cycle test at temperatures of 4°C and 40°C for three cycles. The findings indicate no significant alteration in the organoleptic properties and pH of the gel formulations before and after stability testing. However, t-test analysis reveals a noteworthy decrease in viscosity for carbomer and its combination, albeit not for HPMC alone. In conclusion, HPMC exhibits superior stability as a gel base compared to Carbomer 940 and their combination.
Analisis Nilai Tambah dan Strategi Pemasaran Pengolahan Jamur Tiram (Pleuratus Ostreatus) menjadi Produk Kaldu Jamur di PT. Mitra Jamur Indonesia Sari, Cindy Puspita; Prawitasari, Saptya; Murwanti, Retno
Agri Analytics Journal Vol. 3 No. 1 (2025): June
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/agri.v3i1.3455

Abstract

Produk jamur tiram merupakan komoditas pertanian yang memiliki potensi ekonomi tinggi karena kandungan gizi yang baik dan ketersediannya melimpah, pengolahan jamur tiram segar sering menghadapi tantangan seperti umur simpan yang pendek dan fluktuasi harga di pasaran, sehingga perlu adanya suatu pengolahan untuk memberikan nilai tambah. Tujuan penelitian ini yaitu untuk menganalisis nilai tambah dan keuntungan dalam pengolahan jamur tiram (Pleurotus ostreatus) menjadi kaldu jamur di PT. Mitra Jamur Indonesia serta mengidentifikasi strategi pemasaran yang relevan. Metode penelitian yang digunakan meliputi metode kuantitatif dan kualitatif. Analisis nilai tambah dilakukan dengan menggunakan metode Hayami, analisis SWOT digunakan untuk merumuskan strategi pemasaran berdasarkan faktor internal dan eksternal. Hasil penelitian menunjukkan (1) Pengolahan jamur tiram menjadi kaldu jamur menghasilkan nilai tambah yang positif sebesar Rp. 31.685/kg, dengan rasio nilai tambah sebesar 52%, (2) Keuntungan pada produksi kaldu jamur sebesar Rp. 22.256.506/kg, (3) PT. Mitra Jamur Indonesia berada pada posisi kuadran I Strategi Agresif, strategi yang diusulkan mencakup optimalisasi media sosial, inovasi kemasan, serta kolaborasi dengan distributor untuk memperluas jangkauan pasar.
Current Advances in Triple-Negative Breast Cancer (TNBC) Chemotherapy: A Literature Review Murwanti, Retno; Rommy; Nugroho, Agung Endro
Indonesian Journal of Pharmacology and Therapy Vol 5 No 3 (2024)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.11403

Abstract

Triple-negative breast cancer (TNBC) is one of subtypes of cancer with the most associated death. With more than 20% of breast cancer patients having this subtype, an effective and safe chemotherapy is needed to ensure complete pathomorphological response. Currently, therapies for TNBC patients are divided into systemic therapy regimens for operable or non-operable diseases, with non-operable diseases mostly treated with targeted therapies such as pembrolizumab in tandem with other chemotherapy agents such as paclitaxel, gemcitabine or carboplatin. TNBC patients whom tumor is operable underwent additional step such as preoperative systemic therapy, which offers several advantages than chemotherapy alone. Neoadjuvant chemotherapy (NACT), which consist of mainly drugs from therapeutic classes such as anthracycline and taxanes, is used with limited success (23%) and decreased recurrence rates. Optimal method for treating TNBC is still lacking, therefore the need for further research regarding chemotherapy for TNBC with higher success rate and better prognosis for the patients is paramount.
Pengaruh Amilum Singkong (Manihot esculenta, Crantz) Native dan Pregelatinized Terhadap Laju Disolusi Tablet Parasetamol Ngurah Agung Dewantara Putra, I Gusti; Murwanti, Retno; Rohman, Abdul; Nanda Saifullah Sulaiman, Teuku
Jurnal Farmasi (Journal of Pharmacy) Vol. 7 No. 1 (2018): Jurnal Farmasi (Journal of Pharmacy), October 2018
Publisher : Jurnal Farmasi (Journal of Pharmacy)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Amylum is widely used as an excipient in pharmaceutical formulations, one of which is inert and can be mixed with any drug without causing a chemical reaction. The aim of this research is to know the preparation method of cassava amylum (Manihot esculenta, Crantz) native and pregelatinized which is used as binder to dissolution rate of paracetamol tablet with granulation method. The native and pregelatinized amylum produced from cassava is carried out by a preliminary test to determine the nature of physical characteristics and to know the ability as a binder of the tablet. The paracetamol tablets produced from the two methods were tested dissolution using medium buffer phosphate at 37 ± 0,5 ° C (pH 5.8, 50 rpm). The results showed that cassava starch preparation method had an effect on dissolution rate of tablet. Pregelatinized amylum as binder on paracetamol tablet showed Q value (93,17 ± 0,01)% while native amylum showed value of Q (81,76 ± 0,20)%. The use of pregelatinized cassava starch as a binder on paracetamol tablet has better characteristic properties compared with native starch.
PEMBINAAN BUDIDAYA PERTANIAN BERBASIS SMART VERTICAL FARMING UNTUK PEMANFAATAN LAHAN SEMPIT DI DAERAH PERUMAHAN Cahyanto, Triawan Adi; Murwanti, Retno
INTEGRITAS : Jurnal Pengabdian Vol 6 No 1 (2022): JANUARI - JULI
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat - Universitas Abdurachman Saleh Situbondo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36841/integritas.v6i1.1533

Abstract

Pembangunan perumahan di perkotaan yang sangat pesat, cenderung tidak mempertimbangkan faktor konservasi lingkungan dengan memberikan sumbangsih ruang terbuka hijau yang terbatas. Pekarangan merupakan lahan yang potensial untuk dikembangkan menjadi lahan pertanian produktif terutama untuk pemenuhan kebutuhan pangan yang bergizi bagi si pemilik. Keterbatasan lahan bukanlah hal yang menjadi hambatan untuk mengaktualisasi potensi nilai ekonomi. Lahan di lingkungan perumahan yang cenderung terbatas dapat dioptimalkan untuk ditanami tanaman yang memiliki nilai ekonomi tinggi seperti tanaman pangan, tanaman hias, tanaman obat dan tanaman penyuplai oksigen dalam jumlah besar. Salah satu model optimalisasi lahan terbatas untuk mendukung pertanian sederhana di lingkungan perumahan yaitu model smart vertical farming. Metode pelaksanaan kegiatan antara lain dengan memberikan pelatihan teknik vertical farming, praktik budidaya vertical farming, pembuatan model smart vertical farming dan cara melakukan perawatan. Paparan pelatihan dan praktik disampaikan oleh narasumber dari bidang agribisnis, sedangkan pemodelan smart vertical farming disampaikan oleh narasumber dari bidang teknik informatika. Opini yang berkembang di masyarakat bahwa menerapkan konsep vertical farming adalah sesuatu yang mahal dan rumit, nyatanya tidak semuanya benar. Dengan bermodalkan bibit tanaman, paralon atau botol bekas, maka masyarakat dapat membuat model pertanian vertikal. Model tersebut akan menjadi lebih efisien apabila menerapkan konsep pertanian cerdas berbasis sensor dalam pemantauan dan pengelolaan tanaman. Hasil dari kegiatan baik pelatihan, praktik, maupun pembuatan model smart vertical farming sangat diterima dengan baik oleh masyarakat.
The quality of life (QoL) of advanced stage lung adenocarcinoma patients receiving afatinib at Dr. Sardjito General Hospital, Yogyakarta Bayu Prio Septiantoro; Retno Murwanti; Dyah Aryani Perwitasari; Mustofa
Indonesian Journal of Pharmacology and Therapy Vol 6 No 2 (2025)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.20381

Abstract

Lung cancer has the highest mortality rate of all cancers. In its advanced stages, this disease is associated with poor survival rates and quality of life. The most common subtype of lung cancer is adenocarcinoma which is frequently associated with mutations in the epidermal growth factor receptor (EGFR), a protein crucialfor cell proliferation. The presence of EGFR mutations plays a key role in targeted therapy using tyrosine kinase inhibitors (TKIs). Afatinib is a second-generation TKI that offers several advantages over the first-generation. Afatinib is effective against complex and uncommon EGFR mutations, making it a commonly usedoption for patients with lung adenocarcinoma. However, afatinib is also associated with a higher incidence of adverse drug reactions (ADRs) compared to other TKIs, which may impact therapeutic outcomes. This study aimed to assess the quality of life (QoL) of advanced lung adenocarcinoma patients with EGFR mutations andthe prevalence of ADRs associated with afatinib treatment at Dr. Sardjito General Hospital in Yogyakarta. The study employed an observational cross-sectional design. Prospective data collection was conducted for two months, from January to February 2025 at the Oncology Department following ethical approval. A totalof 15 patients were enrolled in this study. The most commonly identified ADRs were acne (45%), diarrhea (20%), paronychia (20%), and oral mucositis (15%). The mean QoL scores was 81.38 ± 10.01 for the functional scale, 15.93 ± 8.48 for the symptom scale, and 72.78 ± 19.53 for global health status.
Aktivitas Penghambatan enzim Dipeptidyl Peptidase 4 (DPP-4) dari ekstrak dan seskuiterpen lakton yang Diisolasi dari Daun Yacon (Smallanthus sonchifolius) Tamhid, Hady Anshory; Hertiani, Triana; Murti, Yosi Bayu; Murwanti, Retno
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 6, ISSUE 2, October 2025
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/EKSAKTA.vol6.iss2.art2

Abstract

The Sesquiterpene lactones, enhydrin and uvedalin, are the chemotype and subtype compounds of the yacon leaf. They are known to have anti-diabetic activity, but their mechanisms are not clear. One of the mechanisms of anti-diabetic agents is to inhibit the activity of the dipeptidyl peptidase 4 enzyme (DPP-4). The research aimed to determine the inhibition of the extract, enhydrin, and uvedalin isolated from the yacon leaf against the DPP-4 enzyme activity. Yakon leaves were extracted using 70% ethanol by the maceration method. Enhydrin and uvedalin were obtained from previous research. The inhibitory activity of the DPP-4 enzyme was then determined by the fluorescence assay method using a multi-well plate reader. Sitagliptin was used as standard inhibitor of DPP-4 enzyme. The ethanol extract of yacon leaves inhibits the DPP-4 enzyme with an IC50 of 856.9 ppm. The percentage inhibition of the DPP-4 enzyme by the enhydrin and uvedalin at a concentration of 250 ppm was 2.37 and 35.16%, respectively. The inhibitory potency of the pure isolated compounds was not substantially greater than that of the crude extract itself. This led us to the conclusion that the overall contribution of enhydrin and uvedalin to the extract's DPP-4 inhibitory activity is actually modest, suggesting the presence of other active compounds within the extract. However, it may provide a new approach to the treatment of type 2 diabetes.
The Role of Lactiplantibacillus plantarum in Modulating the Gut-Skin Axis: A Comprehensive Review on Its Potential in Managing Atopic Dermatitis Cahyo Nurani, Sekar; Novrizal Abdi Sahid , Muhammad; Murwanti, Retno
Journal of Food and Pharmaceutical Sciences Vol 13, No 3 (2025): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.20639

Abstract

Atopic Dermatitis (AD) is a chronic inflammatory skin condition marked by impaired skin barrier function, immune system irregularities, and microbial imbalance. This research examines the efficacy of Lactiplantibacillus plantarum, a probiotic recognized for its established safety and advantageous characteristics, in the management of atopic dermatitis (AD). The pathophysiology of AD encompasses genetic, environmental, and immunological components, with dysbiosis of skin and gut microbiota being essential. L. plantarum has shown beneficial effects in controlling gut and skin microbiota, improving skin barrier integrity, and modulating immunological responses via processes including short-chain fatty acid (SCFA) synthesis, cytokine regulation, and competitive exclusion of pathogens. Clinical investigations demonstrate that L. plantarum supplementation can diminish the severity of atopic dermatitis, as assessed by the SCORAD index, and enhance skin barrier integrity. The effectiveness of probiotic therapy may be affected by genetic, nutritional, and environmental variables. This review emphasizes the promise of L. plantarum as a therapeutic agent for AD, while also discussing the limitations and future possibilities for probiotic-based therapies in managing this intricate condition.
KESEMBUHAN LUKA SETELAH PEMBERIAN TOPIKAL ZINK PADA TIKUS DENGAN PAKAN LEMAK TINGGI Anggraeni, Devita; Adji, Dhirgo; Murwanti, Retno
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2809

Abstract

Penelitian ini bertujuan mengetahui peran zink pada kesembuhan luka dalam kondisi berat badan berlebih. Dalam penelitian digunakan 20 ekor tikus Sprague Dawley jantan berumur tiga bulan (150-200 g). Tikus dibagi secara acak menjadi empat kelompok (A, B, C, dan D), masing-masing kelompok terdiri atas lima ekor. Kelompok A dan B diberi pakan normal, sedangkan kelompok C dan D diberi pakan lemak tinggi. Setelah dua bulan perlakuan pakan, seluruh tikus dioperasi untuk membuat luka irisan pada kulit, kemudian luka ditutup dengan jahitan. Luka jahitan pada kelompok A dan C diberi aplikasi topikal unguenta vaseline album (tanpa zink), sedangkan luka jahitan pada kelompok B dan D diberi aplikasi topikal unguenta zink 10% dengan bahan dasar vaselin album. Lima hari setelah operasi, seluruh tikus diambil darahnya untuk dianalisis terhadap jumlah leukosit total kemudian diikuti pengambilan jaringan kulit untuk pemeriksaan histopatologis dan penghitungan jumlah leukosit di jaringan. Hasil penelitian menunjukkan adanya interaksi yang signifikan antara pakan dan aplikasi topikal terhadap jumlah leukosittotal dalam darah (P0,05). Jumlah leukosit di jaringan mengalami penurunan setelah aplikasi topikal zink, meskipun hasilnya tidak signifikan (P0,05). Analisis histopatologis menunjukkan epidermis sudah tertutup pada seluruh tikus perlakuan, namun demikian sebagian tikus masih menunjukkan dermis yang terbuka, yaitu kelompok A sebanyak 60%, kelompok B sebanyak 60%, kelompok C sebanyak 60%, dan kelompok D sebanyak 40%. Dapat disimpulkan bahwa proses kesembuhan luka setelah pemberian topikal zink pada tikus yang diberi pakan lemak tinggi menunjukkan hasil yang baik, yaitu ditandai dengan penutupan lapisan epidermis dan dermis, serta menurunnya jumlah leukosit di jaringan.
Apoptosis Induction of SKOV-3 Ovarian Cancer Cells from Pacing Rhizome (Costus speciosus) Through the Modulation of BAX and P53 Genes Expression Hidayah, Alifia Brilliani; Ashfannada, Nuqya; Khasanah, Aisyah Nur; Annisa, Siti Nur; Tiffany, Ghea Rachella; Murwanti, Retno
Indonesian Journal of Cancer Chemoprevention Vol 14, No 2 (2023)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev14iss2pp139-150

Abstract

Costus speciosus or Pacing has been investigated to have cytotoxic effect on several human cancer cells. Current was conducted to determine the cytotoxic activity of ethanol extract of the Pacing rhizome (EP) on SKOV-3 ovarian cancer cells, to investigate EP’s effect on BAX and p53 expression, and predict the inhibition activity on intrinsic pathway. EP were extracted using maceration method with 70% ethanol. The cytotoxic effect was performed using MTT Assay with various concentrations (375 μg/mL, 250 μg/mL, 62.5 μg/mL, 31.25 μg/mL, and 7.8125 μg/mL). The ability of EP to induce apoptosis in SKOV-3 cells was measured using flow cytometry. The BAX and p53 gene expressions on SKOV-3 cells were detected with RT-qPCR after treatment of EP with concentrations of ¼ IC50, ½ IC50, and IC50. The ability of diosgenin to interact with BAX and p53 can be seen from the interaction with the MCL-1 protein and was predicted with molecular docking. The results showed that IC50 of cytotoxic activity was 69.143 μg/mL. EP could induce apoptosis on SKOV-3 cells and can induce BAX and p53 protein expressions. The docking results show that diosgenin has the potential to act as an antagonist for the MCL-1 protein, it can increase apoptosis.Keywords: apoptotic, Costus speciosus extract, SKOV-3, p53, BAX.
Co-Authors Abdul Rohman Adam Hermawan Agung Endro Nugroho Agung Endro Nugroho Ahmad Marzuki Alexxander, . Alexxander, . Amalia, Latifa Anami Riastri Andayana Puspitasari Gani Andayana Puspitasari Gani Andayana Puspitasari Gani Annisa, Siti Nur Anselma Ivanawati Arief Nurrochmad Arief Nurrochmad Arief Nurrochmad Arief Nurrochmad Arief Rahman Hakim Arifka, Vigha Ilmanafi Aris Haryanto Ariska Deffy Anggarany, Ariska Deffy Ashfannada, Nuqya Asri Mega Putri Azizah Amin Azmi Rahmadani Bambang Sulistiyo Ari Sudarmanto Bani Adlina Shabrina Bayu Irawan, Muhamad Bayu Prio Septiantoro Beni Lestari Budastra, Wayan Cintya Ganes Cahyo Nurani, Sekar Cindy Puspita Sari Devina Martina Devita Anggraeni Devita Anggraeni Devyanto Hadi Triutomo Dewi Kartikawati Paramita Dhania Novitasari Dhirgo Adji Dhirgo Adji Diana Rachma Ningsih Dwi Brilyani Sandy Dwi Salim, Rozin Dyah Afritasari Dyah Aryani Perwitasari Dyah Widiastuti Dyaningtyas Dewi Pamungkas Putri Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Effendi, Adha Maula Erna Prawita Setyowati Erna Prawita Setyowati Fikriansyah Fikriansyah Fita Rahmawati Fitri, Dafina Aisya Fitriana Hayyu Arifah Gagas Pradani Nur Ilmawati Gani, Andayana Puspitasari Ginna Zabrina Hayati, Farida Hendri Wasito Herwandhani Putri Hidayah, Alifia Brilliani Hilyatul Fadliyah I Gusti Ngurah Agung Dewantara Putra Ida Ayu Putu Sri Widnyani Idlohatud Dilalah Ika Puspita Sari Illian, Didi Nurhadi Imono Argo Donatus, Imono Argo Ina Kusrini Iren Wati Siahaan Jenie, Riris Istighfari Juang Juansa Khafi, Muhammad Khasanah, Aisyah Nur Khoerul Anwar Khoerul Anwar Kustanto, Satya Prima Layung Sekar Sih Wikanthi Ledi, Ledi Klarismawati Lodyta Nawang Tika lubis, muhammad fauzan Lukman Hakim Luthfiyyah, Annisa Tiara Martien , Ronny Mentari Widiastuti Miftahus Sa'adah Mustofa Mustofa Naisbitt Iman Hanif, Naisbitt Iman Nanang Fakhrudin Nanang Fakhrudin, Nanang Nanda Saifullah Sulaiman, Teuku Nastiti Wijayanti Ngurah Agung Dewantara Putra, I Gusti Nindi Wulandari Nindya Budiana Putri Nindya Budiana Putri Ningsih, Diana Rachma Norita Citra Yuliarti, Norita Citra Novrizal Abdi Sahid , Muhammad Nursani, Rabila Nurul Fathiyah F Nurul Mukhlisa Pratiwi, Rima Dwi Prawitasari, Saptya Prisnu Tirtanirmala Probowulan, Diyah Purnomo, Kurnia Rahayu Purwantiningsih Purwantiningsih Rahmawaty Rachmady Rahmayani, Almira Retno S. Sudibyo Retno Sunarminingsih Sudibyo Riandari , Teti Mariam Rima Munawaroh Risa Umari Yuli Aliviyanti Ritmaleni, Ritmaleni Rizkita, Anggraini Ihza Rohmad Yudi Utomo Rohman, Abdul Rommy Sagala, Reynelda Juliani Sardjiman . Sari Haryanti Sari Haryanti Sari, Laras Ratna Shofa Annur Siswadi Siswadi Sri Susilowati Sri Tasminatun Sri Wahdaningsih Subagus Wahyuono Sudarsono Sudarsono Sugeng Riyanto Sugiyanto . Sugiyanto . Sulaiman, T.N Saifullah Sumaiyah, Sumaiyah Susi Ari Kristina Suwijiyo Pramono Suwijiyo Pramono Tamhid, Hady Anshory Tampubolon, Keshia Thoriq Ziyad, Thoriq Tiffany, Ghea Rachella Triana Candraningrum Triana Hertiani Triawan Adi Cahyanto Ulfa Afrinurfadhilah Darojati Wahyu Utaminingrum Widya Leontin Susanti Yoce Aprianto Yosi Bayu Murti Yundari, Yundari Zahrotul Ulum Ziana Walidah, Ziana