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Apoptosis Induction of SKOV-3 Ovarian Cancer Cells from Pacing Rhizome (Costus speciosus) Through the Modulation of BAX and P53 Genes Expression Alifia Brilliani Hidayah; Nuqya Ashfannada; Aisyah Nur Khasanah; Siti Nur Annisa; Ghea Rachella Tiffany; Retno Murwanti
Indonesian Journal of Cancer Chemoprevention Vol 14, No 2 (2023)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev14iss2pp139-150

Abstract

Costus speciosus or Pacing has been investigated to have cytotoxic effect on several human cancer cells. Current was conducted to determine the cytotoxic activity of ethanol extract of the Pacing rhizome (EP) on SKOV-3 ovarian cancer cells, to investigate EP’s effect on BAX and p53 expression, and predict the inhibition activity on intrinsic pathway. EP were extracted using maceration method with 70% ethanol. The cytotoxic effect was performed using MTT Assay with various concentrations (375 μg/mL, 250 μg/mL, 62.5 μg/mL, 31.25 μg/mL, and 7.8125 μg/mL). The ability of EP to induce apoptosis in SKOV-3 cells was measured using flow cytometry. The BAX and p53 gene expressions on SKOV-3 cells were detected with RT-qPCR after treatment of EP with concentrations of ¼ IC50, ½ IC50, and IC50. The ability of diosgenin to interact with BAX and p53 can be seen from the interaction with the MCL-1 protein and was predicted with molecular docking. The results showed that IC50 of cytotoxic activity was 69.143 μg/mL. EP could induce apoptosis on SKOV-3 cells and can induce BAX and p53 protein expressions. The docking results show that diosgenin has the potential to act as an antagonist for the MCL-1 protein, it can increase apoptosis.Keywords: apoptotic, Costus speciosus extract, SKOV-3, p53, BAX.
ASSOCIATION OF ZINGIBER AROMATICUM VAL. WITH ZERUMBONE AND ITS BIOLOGICAL ACTIVITY: A LITERATURE REVIEW Murwanti, Retno; Pratiwi, Rima Dwi; Gani, Andayana Puspitasari
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 17 No 1 (2023): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33533/jpm.v17i1.5816

Abstract

The main objective of this article is to explore the available classified information on Zingiber aromaticum Val. Rhizome and its active compound, Zerumbone. Throughout history, this plant has been utilized to treat various ailments such as abdominal disorders, cough, anemia, malaria, jaundice, arthritis, and infections. Recently, Zerumbone, a sesquiterpene compound found in the rhizomes, has gained attention for its biological activity. This article reviews the current research on the pharmacological activities of Z. aromaticum Val. Rhizome, with a specific focus on its active component, Zerumbone.This review found that the antioxidant and antiinflammatory effects of Zerumbone play a role in the pharmacological effects exhibited by Z. aromaticum, such as antidiabetic, antiparasitic, anti-hyperlipidemia, antianxiety, and antiaging effects. Additionally, the correlation between the biological activity of Zerumbone and the pharmacological activity of Z. aromaticum extract is discussed. This review provides a valuable reference for developing and further researching Zingiber aromaticum Val. and Zerumbone in medical treatments.
Optimalisasi Pemanfaatan Daun Kelor Sebagai Produk Olahan Bernilai Gizi dan Bernilai Ekonomi Tinggi Maharani, Astrid; Murwanti, Retno
Journal of Community Development Vol. 2 No. 1 (2021): October
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (469.713 KB) | DOI: 10.47134/comdev.v2i1.34

Abstract

Moringa oleifera is the one of familiar plants in Harjomulyo Village. The one factor of the number moringa plants in Harjomulyo Village because it’s too easy to grow in tropical climates, have high rainfall and are resistant to dry seasons. The problem of Harjomulyo Village is a lack of knowledge related to nutritional value and high economic value related to moringa plants which are widely available in Harjomulyo Village. The aim of this service is to educate the Moringa plant into processed nutritious food that has high economic value for the people of Harjomulyo Village. In solving the problem, it is carried out by extension methods and discussions about the benefits of moringa plants for health and fulfillment nutrition for family, technical counseling on the process of moringa sticks, packaging of moringa sticks, and business and marketing analysis of moringa sticks.
Profil Penggunaan Granulocyte Colony Stimulating Factor (G-Csf) Pada Pasien Kanker Dengan Neutropenia di RSUP Dr. Kariadi Semarang Ningsih, Diana Rachma; Murwanti, Retno; Nugroho, Agung Endro
Majalah Farmaseutik Vol 20, No 1 (2024)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/farmaseutik.v20i1.86467

Abstract

Kejadian neutropenia pada pasien kanker disebabkan 90% akibat kemoterapi. Penundaan pengatasan neutropenia dapat menyebabkan risiko terjadinya febril neutropenia sehingga menyebabkan tingginya mortalitas. Pasien kanker dengan neutropenia dapat diberikan terapi granulocyte colony stimulating factor (G-CSF), yang dapat mengurangi kejadian febril neutropenia, lama perawatan, menurunkan jumlah angka kesakitan dan kematian pada pasien kanker. Tujuan dari penelitian ini adalah mengetahui profil penggunaan G-CSF pada pasien kanker dengan neutropenia. G-CSF yang digunakan adalah Filgrastrim dan Lenograstrim. Penelitian ini menggunakan Retrospective Cohort Study dengan observational analitik yang dilaksanakan di RSUP Dr. Kariadi Semarang. Data penelitian dikumpulkan secara retrospektif menggunakan catatan medik pada periode 2019-2022. Sampel penelitian diperoleh 56 subyek yang memenuhi kriteria inklusi. Berdasarkan hasil penelitian pasien kanker dengan neutropenia  lebih banyak terjadi pada pasien kanker non hematologi sebesar (62,5%) dengan rentang usia paling banyak terjadi pada pada 18-60 tahun (78,6%). Grade neutropenia yang diamali pasien paling banyak yaitu neutropenia severe (55,3%), sedangkan pada neutropenia moderatre sejumlah (32,2%), dan neutropenia mild sebesar (12,5%). Profil penggunaan G-CSF pada pasien kanker dengan neutropenia di RSUP Dr. Kariadi yang diteliti yaitu Filgrastrim dan Lenograstrim. Pasien yang diberikan Filgrastrim sejumlah (73,2%) dan yang diberikan Lenograstrim sejumlah (26,8%). 
Kajian Efek Samping Pada Pasien Kanker Payudara Dengan Regimen Paclitaxel-Epirubicin-Fluorouracil di RSUD Kabupaten Temanggung Kustanto, Satya Prima; Murwanti, Retno; Nugroho, Agung Endro
Majalah Farmaseutik Vol 19, No 4 (2023)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/farmaseutik.v19i4.89064

Abstract

Kanker payudara merupakan salah satu kanker yang banyak terjadi dan sering menyebabkan kematian tertingi pada wanita. Kemoterapi merupakan salah satu modalitas terapi yang direkomendasikan pada pasien kanker payudara yang dinilai efektif namun menghasilkan efek samping dan berefek toksik pada sel normal. Penelitian ini bertujuan  untuk mengetahui gambaran kejadian efek samping obat hematologi dan non hematologi yang menjalani kemoterapi. Penelitian ini dilakukan di RSUD Kabupaten Temanggung pada bulan Juni-Juli 2023 dengan rancangan cohort retrospective study melalui data sekunder dari rekam medis dan data laboratorium. Dari 47 pasien yang menjalani kemoterapi mingguan dengan regimen kombinasi paclitaxel 60mg/m2, epirubicin 50mg/m2 dan fluorouracil 300mg/m2 diamati, kejadian efek samping hematologi yang paling banyak dialami oleh pasien adalah anemia sebanyak 70.2%, leukopenia 55.3%, neutropenia 27.7% dan peningkatan SGOT dan SGPT 12.8%. Efek samping non hematologi yang paling banyak terjadi adalah rambut rontok 91.5%, mual 82.9%, stomatitis 61.7%, kuku menghitam 48.9%, reaksi hipersensitivitas 46.8%, neuropati perifer 31.9%, sembelit 27.7% dan diare 10.6%.
Narative Review: Kejadian Dan Mekanisme Kardiotoksisitas Pada Pasien Kanker Payudara Setelah Penggunaan Trastuzumab Sari, Laras Ratna; Murwanti, Retno; Rahmawati, Fita
Majalah Farmaseutik Vol 20, No 3 (2024)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/farmaseutik.v20i3.97047

Abstract

Kardiotoksisitas adalah terjadinya disfungsi jantung sebagai kerusakan listrik atau otot, yang mengakibatkan jantung menjadi lebih lemah dan tidak efisien dalam memompa darah. Kardiotoksisitas dapat disebabkan oleh beberapa hal seperti agen kemoterapi, pengobatan radioterapi, komplikasi dari anoreksia nervosa, efek samping dari asupan logam berat. Kardiotoksisitas juga terjadi pada pasien kanker payudara yang diberikan terapi trastuzumab. Tujuan dari tinjauan artikel ini yaitu untuk mengetahui kejadian kardiotoksisitas setelah penggunaan trastuzumab dan mekanisme efek kardiotoksisitas trastuzumab pada pasien kanker payudara. Metode yang digunakan pada artikel ini yaitu menggunakan beberapa pencarian menggunakan PubMed, ScienceDirect dan Google Cendekia untuk penelitian yang membahas efek kardiotoksisitas trastuzumab pada pasien kanker payudara dari tahun 2018 sampai dengan 2023. Dari total 171 artikel, yang kemudian dievaluasi berdasarkan kriteria inklusi dan eksklusi. Sebanyak 157 artikel dikeluarkan dan 14 artikel yang memenuhi kriteria inklusi. Mekanisme terjadinya kardiotoksisitas oleh trastuzumab melalui mekanime penghambatan HER2 dan autophagy. Kesimpulannya, Pemilihan trastuzumab sebagai pilihan terapi pada pasien kanker payudara harus dipertimbangkan dengan cermat dan diperlukan pemantauan fungsi jantung.  
Analyzing the Potential of Herbal Plant Research for Alzheimer's Disease in Diabetes: A Bibliometric Approach Marzuki, Ahmad; Rahmayani, Almira; Khafi, Muhammad; Hertiani, Triana; Murwanti, Retno
Majalah Farmaseutik Vol 20, No 3 (2024)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/farmaseutik.v20i3.97209

Abstract

Alzheimer’s disease is a progressive neurologic dementia disorder and the fifth leading cause of death among elderly individuals. Its pathogenesis is closely linked to diabetes-induced brain insulin resistance, which accelerates beta-amyloid accumulation, exacerbating disease severity. This condition significantly impacts quality of life and healthcare costs, necessitating effective treatment strategies. Medicinal plants offer a promising avenue, with bibliometric analysis identifying trending active compounds for potential development as therapies for diabetic Alzheimer’s. This study analyzed 200 articles from the Scopus database, employing Scopus and VOSviewer 1.6.20 to visualize bibliometric trends by publication, country/territory, subject area, and keyword co-occurrence. Results indicate a recent decline in research activity, with China leading in publications across pharmacology, medicine, and biochemistry. Notably, flavonoids like “quercetin,” “kaempferol,” and “gallic acid” emerged as prominent topics.Keywords: Alzheimer’s diabetes, Bibliometrics, Herbal medicine, Extracts
Kajian Pustaka: Sediaan Kosmesetika Penumbuh Rambut dari Berbagai Herbal Nusantara Budastra, Wayan Cintya Ganes; Riandari , Teti Mariam; Martien , Ronny; Murwanti, Retno
MPI (Media Pharmaceutica Indonesiana) Vol. 5 No. 1 (2023): JUNE
Publisher : Fakultas Farmasi, Universitas Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24123/mpi.v5i1.5594

Abstract

Kerontokan rambut dapat menjadi permasalahan serius ketika dalam jumlah yang abnormal dan terjadi secara terus-menerus hingga berujung kebotakan. Kosmesetika penumbuh rambut berbahan dasar herbal dapat menjadi pilihan terapi yang tepat sebab terkait dengan keamanan penggunaan jangka panjang maupun efektivitasnya. Berbagai bentuk sediaan kosmesetika herbal penumbuh rambut telah dikembangkan yakni emulsi, gel, emulgel, krim, serum, hair tonic maupun sediaan yang dimodifikasi dengan sistem teknologi nano. Artikel review ini bertujuan untuk mengetahui sediaan kosmesetika herbal terbaik yang memenuhi spesifikasi farmasetik yang telah ditetapkan dan aktivitasnya sebagai penumbuh rambut secara in vivo. Penelitian ini berbasis systematic literature review. Berdasarkan eksplorasi literatur yang telah dilakukan dapat diketahui bahwa sediaan kosmesetika herbal terbaik dalam meningkatkan pertumbuhan rambut dan memenuhi kriteria karakteristik farmasetik adalah adalah sediaan hair tonic daun kangkung (Ipomoea aquatica Forsk.) (5%) dengan rerata peningkatan panjang rambut sebesar (2,785 ± 0,021 cm) dan bobot rambut (0,767 ± 0,057 mg/cm2) selama 21 hari. Evaluasi farmasetik hair tonic daun kangkung telah memenuhi persyaratan SNI sediaan hair tonic yang telah ditetapkan. Hair loss can be a serious problem when it is in abnormal amounts and occurs continuously leading to baldness. Herbal-based hair growth cosmeceuticals can be the right choice of therapy because it is related to the safety of long-term use and its effectiveness. Various dosage forms of herbal hair growth cosmeceuticals have been developed, namely emulsions, gels, emulgels, creams, serums, hair tonic and preparations modified with nano technology systems. This review article aims to determine the best herbal cosmeceutical preparations that meet the established pharmaceutical specifications and their activity as hair growth in vivo. This research is based on systematic literature review. Based on the literature exploration that has been carried out, it can be seen that the best herbal cosmeceutical preparation in increasing hair growth and meeting the criteria for pharmaceutical characteristics is the preparation of kale leaf hair tonic (Ipomoea aquatica Forsk.) (5%) with an average increase in hair length of (2.785 ± 0.021 cm) and hair weight (0.767 ± 0.057 mg/cm2) in 21 days. Pharmaceutical evaluation of kale leaf hair tonic has met the requirements of SNI hair tonic preparations that have been established.
Pengaruh G-CSF terhadap ANC recoverypada pasien kanker dengan neutropenia Ningsih, Diana Rachma; Murwanti, Retno; Nugroho, Agung Endro
MEDIA ILMU KESEHATAN Vol 12 No 2 (2023): Media Ilmu Kesehatan
Publisher : Universitas Jenderal Achmad Yani Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/mik.v12i2.958

Abstract

Background: Neutropenia can increase morbidity in cancer patients because it can cause an increased risk of infection, delay or discontinue chemotherapy, and decrease the dose of cytotoxic agents which affect the patient's response and clinical outcome. The administration of G-CSF can increase ANC levels in neutropenic patients. The effectiveness of G-CSF was assessed by increasing ANC levels compared to before the administration of G-CSF.Objective: This study aims to determine the effect of G-CSF administration on ANC levels in cancer patients with neutropeniaMethods: This research was conducted at Dr. Kariadi General Hospital Semarang in October-December 2022 period with retrospective cohort method. The sample was cancer patients who experienced neutropenia and were given G-CSF.Results: Administration of G-CSF significantly increased ANC values (p-value 0.00 <0.05), increased by 921.07 ± 647.20 in the administration of filgrastim and 933.13 ± 558.89 in the administration of lenograstim and shortened the duration of ANC recovery (p-value 0.00 <0.05), recovery was 1.5 days shorter with filgrastim and 1.9 days with lenograstim.Conclusion: G-CSF Filgrastim and Lenograstim administration can increase ANC levels and shorten the duration of neutropenia treatment
New Insights of Response Surface Methodology Approach in Optimizing Total Phenolic Content of Zanthoxylum acanthopodium DC. Fruit Extracted Using Microwave-Assisted Extraction and the Impact to Antioxidant Activity Sumaiyah, Sumaiyah; Murwanti, Retno; Illian, Didi Nurhadi; Lubis, Muhammad Fauzan; Tampubolon, Keshia
Indonesian Journal of Chemistry Vol 24, No 6 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.95922

Abstract

Zanthoxylum acanthopodium DC., a unique spice plant from North Sumatra, is rich in beneficial secondary metabolites, particularly phenolic compounds. This study utilized the microwave-assisted extraction method to enhance the extraction of these bioactive compounds. The goal was to determine the optimal extraction conditions, including solvent concentration (X1), microwave power (X2), and extraction time (X3) to maximize the total phenolic content (TPC) of Z. acanthopodium fruit. A Box-Behnken design, part of response surface methodology, was used with three factors at three levels: X1 (50%, 75%, and 100% ethanol in water), X2 (180, 300, and 450 W), and X3 (3, 8.5, and 14 min). The phenolic compounds in the optimized extract were identified using LC-HRMS, and its antioxidant activity was measured using radical scavenging activity assays. The statistical analysis indicated a significant quadratic model (p-value < 0.05), with a high R2 of 86.25%. Optimal conditions for maximum TPC (159.637 ± 5.72 mg GAE/g) were achieved with 50% ethanol, 450 W, and 8.5 min, outperforming conventional extraction methods. Compared to the non-optimized extract, the optimized extract also exhibited strong antioxidant activity, particularly in DPPH radical inhibition. This method successfully optimized TPC in Z. acanthopodium fruit, enhancing its antioxidant properties.
Co-Authors Abdul Rohman Adam Hermawan Agung Endro Nugroho Agung Endro Nugroho Ahmad Marzuki Aisyah Nur Khasanah Alexxander, . Alexxander, . Alifia Brilliani Hidayah Amalia, Latifa Anami Riastri Andayana Puspitasari Gani Andayana Puspitasari Gani Andayana Puspitasari Gani Anselma Ivanawati Arief Nurrochmad Arief Nurrochmad Arief Nurrochmad Arief Nurrochmad Arief Rahman Hakim Arifka, Vigha Ilmanafi Ariska Deffy Anggarany, Ariska Deffy Asri Mega Putri Azizah Amin Azizah, Ulfah Laily Azmi Rahmadani Bambang Sulistiyo Ari Sudarmanto Bani Adlina Shabrina Bayu Irawan, Muhamad Bayu Prio Septiantoro Beni Lestari Budastra, Wayan Cintya Ganes Cindy Puspita Sari Devina Martina Devita Anggraeni Devyanto Hadi Triutomo Dhania Novitasari Dhirgo Adji Diana Rachma Ningsih Dwi Brilyani Sandy Dwi Salim, Rozin Dyah Afritasari Dyah Aryani Perwitasari Dyaningtyas Dewi Pamungkas Putri Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Effendi, Adha Maula Erna Prawita Setyowati Erna Prawita Setyowati Fikriansyah Fikriansyah Fita Rahmawati Fitri, Dafina Aisya Fitriana Hayyu Arifah Gagas Pradani Nur Ilmawati Gani, Andayana Puspitasari Ghea Rachella Tiffany Ginna Zabrina Hayati, Farida Hendri Wasito Herwandhani Putri Hilyatul Fadliyah I Gusti Ngurah Agung Dewantara Putra Ida Ayu Putu Sri Widnyani Idlohatud Dilalah Ika Puspita Sari Illian, Didi Nurhadi Imono Argo Donatus, Imono Argo Iren Wati Siahaan Jenie, Riris Istighfari Juang Juansa Khafi, Muhammad Kustanto, Satya Prima Layung Sekar Sih Wikanthi Ledi, Ledi Klarismawati Lodyta Nawang Tika lubis, muhammad fauzan Lukman Hakim Luthfiyyah, Annisa Tiara Maharani, Astrid Martien , Ronny Mentari Widiastuti Miftahus Sa&#039;adah Muharom, Luthfi Ali Mustofa Mustofa Naisbitt Iman Hanif, Naisbitt Iman Nanda Saifullah Sulaiman, Teuku Ngurah Agung Dewantara Putra, I Gusti Nindi Wulandari Nindya Budiana Putri Nindya Budiana Putri Ningsih, Diana Rachma Norita Citra Yuliarti, Norita Citra Nunung Yuniarti Nuqya Ashfannada Nursani, Rabila Nurul Mukhlisa Pratiwi, Rima Dwi Prawitasari, Saptya Prisnu Tirtanirmala Probowulan, Diyah Purnomo, Kurnia Rahayu Purwantiningsih Purwantiningsih Rahmawati, Desty Restia Rahmawaty Rachmady Rahmayani, Almira Retno S. Sudibyo Retno Sunarminingsih Sudibyo Riandari , Teti Mariam Rima Munawaroh Riris Istighfari Jenie Risa Umari Yuli Aliviyanti Ritmaleni, Ritmaleni Rizkita, Anggraini Ihza Rohmad Yudi Utomo Rohman, Abdul Rommy Sagala, Reynelda Juliani Sardjiman . Sari Haryanti Sari Haryanti Sari, Laras Ratna Shofa Annur Siswadi Siswadi Siti Nur Annisa Sri Susilowati Sri Tasminatun Sri Wahdaningsih Subagus Wahyuono Sudarsono Sudarsono Sugeng Riyanto Sugiyanto . Sugiyanto . Sulaiman, T.N Saifullah Sumaiyah, Sumaiyah Susi Ari Kristina Suwijiyo Pramono Suwijiyo Pramono Tampubolon, Keshia Thoriq Ziyad, Thoriq Triana Candraningrum Triana Hertiani Triawan Adi Cahyanto Ulfa Afrinurfadhilah Darojati Wahyu Utaminingrum Widya Leontin Susanti Wulandari, Febri Yance Anas Yoce Aprianto Yundari, Yundari Zahrotul Ulum Ziana Walidah, Ziana