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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Jurnal Perikanan Kelautan Jurnal Natur Indonesia Journal of Aquaculture Management and Technology BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Ilmu-Ilmu Perikanan dan Budidaya Perairan Omni-Akuatika Jurnal Perikanan Universitas Gadjah Mada Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Journal of Aquaculture and Fish Health Jurnal Abdi Insani Jurnal Kedokteran Hewan Journal of Fisheries & Marine Biodiversitas Journal of Biological Diversity

Sri Nuryati

Department Of Aquaculture, Faculty Of Fisheries And Marine Sciences, IPB University Bogor, West Java, 16680. Indonesia

Author-ID : 336092

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Growth performance and antioxidant status of giant gourami given different levels of astaxanthin supplementation Sofian, ,; Jusadi, Dedi; Nuryati, Sri
Jurnal Akuakultur Indonesia Vol. 15 No. 1 (2016): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT As stocking density is known as a stress factor in fish. This study aimed to evaluate the effect of dietary astaxanthin supplementation on growth performance and antioxidant status in giant gourami under normal (100 fish/m3) and high (400 fish/m3) stocking density. Addition of astaxanthin in the feed was done by coating method. The experimental fish used were giant gourami with average body weight of 3.0±0.12 g. Fish were reared for 60 days in 100×50×50 cm3-sized-aquariums containing 150 L of water. Feed was given at satiation method twice a day at 09.00 and 17.00 pm. Results showed that fish reared in high stocking density and fed on the diet supplemented with astaxanthin 100 mg/kg diet produced the best growth performance. Addition of astaxanthin did not affect the antioxidant activity of giant gourami. Keywords: giant gourami, astaxanthin, growth performance, antioxidant status ABSTRAK Padat tebar tinggi merupakan salah satu faktor yang dapat menyebabkan stres pada ikan budidaya. Tujuan penelitian ini adalah untuk mengevaluasi pengaruh penambahan astaxanthin melalui pakan terhadap kinerja pertumbuhan dan status antioksidan ikan gurami pada pemeliharaan kepadatan normal (100 ekor/m3) dan kepadatan tinggi (400 ekor/m3). Penambahan astaxanthin pada pakan dilakukan dengan metode pelapisan (coating). Ikan uji yang digunakan adalah benih ikan gurami berukuran bobot rata-rata 3,0±0,12 g. Ikan dipelihara selama 60 hari dalam akuarium berukuran 100×50×50 cm3 berisi air 150 L. Pakan diberikan secara at satiation dengan frekuensi pemberian pakan sebanyak dua kali sehari pada pukul 09.00 dan 17.00 WIB. Hasil penelitian menunjukkan bahwa ikan yang dipelihara pada kepadatan tinggi dan diberi pakan dengan penambahan astaxanthin sebesar 100 mg/kg pakan menghasilkan kinerja pertumbuhan yang optimal. Penambahan astaxanthin sebesar 100 dan 200 mg/kg pakan tidak mempengaruhi status antioksidan pada ikan gurami. Kata kunci: ikan gurami, astaxanthin, pertumbuhan, status antioksidan

The growth and physiological responses of tambaqui Colossoma macropomum fed on the high dose of clove oil-supplemented diet Puteri, Andi Tiara Eka Diana; Jusadi, Dedi; Nuryati, Sri
Jurnal Akuakultur Indonesia Vol. 15 No. 1 (2016): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT In this study, tambaqui Colossoma macropomum juvenile were given feed supplemented with clove oil Syzygium aromaticum at a dose of 0 and 100 mg/100 g diet. This study purposed to assessment the effect of clove oil supplementation at high dose (100 mg/100 g diet) on growth performance and health status of freshwater tambaqui. As many as 25 fishes with initial body weight 7.62±0.44 g maintained in 15 aquariums for 45 days. Each treatment consisted of three replications. Data were analyzed by t-test. The results showed that tambaqui growth performance on both treatments were not significantly different. However, supplementation of high doses 100 mg clove oil/100 g diet showed reduce on the blood parameters and declining the abundant of intestinal microflora in tambaqui gut, but the types seen more diverse. The observation of fish liver histology showed that hepatocyte cells in the treatment of 100 mg clove oil become smaller and dense compared with 0 mg clove oil treatment. The use of high doses of clove oil does not interfere with the tambaqui growth performance despite the downturn in the physiological responses were observed. Keywords: clove oil, Syzygium aromaticum, Colossoma macropomum, growth performance ABSTRAK Di dalam penelitian ini, benih ikan bawal tawar Colossoma macropomum dipelihara dengan pemberian pakan yang ditambah minyak cengkeh Syzygium aromaticum pada kadar 0 dan 100 mg/100 g pakan. Tujuannya untuk melihat pengaruh penambahan minyak cengkeh di dalam pakan pada dosis tinggi (100 mg/100 g pakan) terhadap performa pertumbuhan dan respons fisiologis ikan bawal. Sebanyak 25 ekor ikan bawal dengan bobot individu awal 7,62 ± 0,44 g dipelihara pada 15 akuarium selama 45 hari. Data yang diperoleh dianalisis dengan uji komparasi t-test antar dua perlakuan dengan tiga ulangan. Hasil penelitian menunjukkan bahwa tidak ada pengaruh dari kedua perlakuan terhadap parameter pertumbuhan. Namun, pemberian pakan yang mengandung minyak cengkeh dosis tinggi 100 mg/100 g pakan mempengaruhi respons fisiologis berupa rendahnya kisaran nilai gambaran darah serta menurunnya jumlah mikroflora di usus ikan bawal, meski jenisnya lebih beragam. Hasil pengamatan histologi hati ikan pada perlakuan 100 mg minyak cengkeh menunjukkan bahwa sel hepatosit berukuran lebih kecil dan terlihat rapat dibandingkan dengan perlakuan 0 mg minyak cengkeh. Penggunaan minyak cengkeh dosis tinggi tidak menganggu performa pertumbuhan ikan bawal meskipun terjadi penurunan pada respons fisiologis yang diamati. Kata penting: minyak cengkeh, Syzygium aromaticum, Colossoma macropomum, performa pertumbuhan

The protective duration of Streptococcus agalactiae vaccine in Nile Tilapia for the prevention of streptococcosis Sukenda, ,; Rusli, ,; Nuryati, Sri; Hidayatullah, Dendi
Jurnal Akuakultur Indonesia Vol. 14 No. 2 (2015): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The aim of this study was to assess the protective duration of Streptococcus agalactiae vaccine against streptococcosis in Nile tilapia. Fish were treated by the whole cell vaccine, ECP vaccine and mixture of whole cell and ECP vaccine. After 14, 28, 42, and 52 day post-vaccination (DPV), the fish were intraperitoneally challenged with 104 cfu/mL S. agalactiae. The results showed mortality rate of whole-cell vaccine (A), ECP vaccine (B) and mix vaccine (C) up to day 42 was significantly (P<0.05) lower than the control treatment, namely 73.33%; 80%; and 76%, respectively. The mortality rate of vaccine treatments A, B, and C on day 56 had no significant difference (P>0.05) with the control. The value of antibody titer vaccine treatments A, B, and C indicate that antigen-antibody reaction on day 28 after the vaccination was significantly (P <0.05) higher than the control that were 3.67; 3.33; and 3.67. Antigen-antibody reaction on day 42 after the vaccination was founded, but did not different significantly (P>0.05) with the control. Bacterial population in treatment A, B, and C in the organs of the fish until the 28th day was still under the control of 104 cfu/mL. S. agalactiae vaccine protection duration is 42 days after the vaccination. Keywords : nile tilapia, Streptococcus agalactiae, duration, vaccine, streptococcosis ABSTRAK Penelitian ini bertujuan untuk menganalisis durasi proteksi dari vaksin Streptococcus agalactiae sebagai pencegahan terhadap streptococcosis pada ikan nila. Ikan divaksinasi dengan vaksin sel utuh, ECP dan gabungan sel utuh dan ECP dari S. agalactiae yang diinjeksi secara intrapetorineal. Ikan diuji tantang S. agalactiae 104 cfu/mL pada hari ke-14, ke-28, ke-42, dan ke-56 pascavaksinasi. Hasil penelitian menunjukkan tingkat mortalitas perlakuan vaksin sel utuh (A), vaksin ECP (B), dan gabungan vaksin sel utuh dengan ECP (C) hingga hari ke-42 masih signifikan (P<0,05) lebih rendah dari perlakuan kontrol yaitu 73,33%; 80%; dan 76%. Tingkat mortalitas perlakuan vaksin A, B, dan C pada hari ke-56 sudah tidak berbeda signifikan (P>0,05) dengan kontrol. Nilai titer antibodi perlakuan vaksin A, B, dan C menunjukkan bahwa reaksi antigen antibodi pada hari ke-28 pascavaksinasi masih signifikan (P<0,05) lebih tinggi dibandingkan kontrol yaitu 3,67; 3,33; dan 3,67. Reaksi antigen antibodi pada hari ke-42 pascavaksinasi masih ditemukan, namun tidak berbeda signifikan (P>0,05) dengan kontrol. Populasi bakteri pada perlakuan A, B, dan C di organ ikan hingga hari ke-28 masih di bawah kontrol 104 cfu/mL. Durasi proteksi vaksin S. agalactiae adalah 42 hari pascavaksinasi. Kata kunci: ikan nila, Streptococcus agalactiae, durasi, vaksin, streptococcosis

Infektivitas parasit Ichtyophthirius multifiliis yang disimpan pada suhu rendah Rahman, ,; Sukenda, ,; Nuryati, Sri; Hidayatullah, Dendi
Jurnal Akuakultur Indonesia Vol. 15 No. 2 (2016): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The aim of this study was to evaluate infectivity of Ichtyophthirius multifiliis which caused white spot disease maintained at low temperature without its host. Briefly, the trophont stage of parasites were subjected at control (28 °C) and lower temperature (9 °C) for 14 consecutive days of observation. The rate of survival, and excystment of descendants were examined descriptively at the last day of observation. Here, the infectivity of parasite then performed by means infecting the model fish Poecilia sphenops (black moly) with escaping theronts. The results revealed that the survival rate and excystment rate of parasite were decreased as maintaining period increased. The final rate of survival, and excystment of parasite were 35% and 33,3% respectively. Additionally, the descendants came out with high abnormality which recognized by weak mobility and lower infectivity (50%) compared to the control (80%). Then, it is concluded that, maintaining I. multifiliis at low temperature without its host for 14 consecutive days will decreased the infectivity. Keywords: white spot, obligat parasite, excystment, infectivity ABSTRAK Penelitian ini bertujuan untuk mengevaluasi infektivitas parasit Ichtyophthirius multifiliis penyebab penyakit bintik putih (white spot) yang dipelihara tanpa inang pada suhu rendah. Parasit dengan stadia trophont dipelihara pada suhu ruang (28 °C) dan suhu rendah (9 °C) selama 14 hari. Selama masa pemeliharaan tersebut tingkat kelulusan hidup, dan tingkat eksismen parasit diukur dan dibandingkan secara deskriptif. Hari terakhir pemeliharaan dilakukan uji tantang pada ikan black moly Poecilia sphenops untuk menilai infektivitas parasit. Hasil penelitian memperlihatkan bahwa tingkat kelulusan hidup dan eksismen parasit semakin menurun dengan bertambahnya masa pemeliharaan. Akhir pengamatan kelangsungan hidup, dan nilai eksismen tersebut berturut-turut adalah 35% dan 33%. Parasit yang disimpan pada suhu rendah selama 14 hari memperlihatkan infektivitas yang lebih rendah (50%) dibandingkan dengan perlakuan kontrol (80%). Kesimpulannya, penyimpanan parasit I. multifiliis pada suhu rendah selama 14 hari dapat menurunkan infektivitas parasit pada inang. Kata kunci: bintik putih, parasit obligat, eksismen, infektivitas

Effectiveness of ambon banana stem juice as immunostimulatory against Aeromonas hydrophila infections in catfish Clarias gariepinus Astria, Qorie; Nuryati, Sri; Nirmala, Kukuh; Alimuddin, Alimuddin
Jurnal Akuakultur Indonesia Vol. 16 No. 2 (2017): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Outbreaks of infectious diseases due to Aeromonas hydrophila in catfish can cause high death rates (80–100%). Fish disease control can be done using phytopharmaceutical to prevent or treat diseases of fish. One of the phytopharmaceutical that known to prevent the fish diseases is ambon banana stem Musa cavendishii var. dwarf Paxton. This study was conducted to test the effectiveness of catfish immersion using banana stem juice as an immunostimulant against bacterial infections A. hydrophila. The observed parameters were a total performance of production, hematological test, and water quality. Immersion of catfish seeds with stem juice was performed at a concentration of 5 mL/L, 13 mL/L, and 21 mL/L for 30 minutes. Each treatment consisted of three replications. A total of 15 fishes were immersed in 1.5 L water. At day-9 after immersion, fish was infected by A. hydrophila bacteria at a dose of 104 cfu/mL. The results showed that fish treated with banana stem juice at a concentration of 13 mL/L had the survival rate of 53.33±6.67% which is higher than positive control (33.33±6.67%). Based on hematology observations on the 3rd day post-challenge test (H12) with A. hydrophila, total erythrocytes, hemoglobin, total leukocytes, phagocytic activity, differential leukocyte, and lysozyme activity was highest in treatment13 mL/L. Lower feed conversion ratio (1.08±0.04) were also obtained in treatment 13 mL/L. Thus Immersion of seeds in stem juice can boost the immune system against infections A. hydrophila. Keywords: Aeromonas hydrophila, ambon banana stem juice, soaking, catfish, immune system ABSTRAK Wabah penyakit akibat infeksi Aeromonas hydrophila pada ikan lele dapat menyebabkan tingkat kematian yang tinggi (80–100%). Penanggulangan penyakit ikan dapat dilakukan menggunakan fitofarmaka untuk mencegah ataupun mengobati penyakit ikan. Salah satu fitofarmaka yang dapat digunakan dalam upaya pencegahan penyakit ikan adalah batang pisang ambon lumut Musa cavendishii var. dwarf Paxton. Penelitian ini bertujuan untuk menguji efektivitas perendaman benih ikan lele dengan menggunakan air perasan batang pisang ambon sebagai imunostimulan terhadap infeksi bakteri A. hydrophila. Parameter yang diamati selama penelitian adalah kinerja produksi, uji hematologi dan pengukuran kualitas air. Perendaman benih ikan lele dengan air perasan dilakukan pada konsentrasi 5 mL/L, 13 mL/L, dan 21 mL/L selama 30 menit. Pada hari ke-9 setelah perendaman, ikan diinfeksi bakteri A. hydrophila pada kepadatan 104 cfu/mL. Hasil penelitian menunjukkan bahwa perendaman ikan dengan air perasan pada konsentrasi 13 mL/L memiliki kelangsungan hidup yakni sebesar 53,33±6,67%, lebih tinggi bila dibandingkan dengan kontrol positif (33,33±6,67%). Berdasarkan pengamatan pada uji hematologi hari ke-3 pascauji tantang (H12) dengan bakteri A. hydrophila, total eritrosit, hemoglobin, total leukosit, aktivitas fagositik, dan aktivitas lisozim tertinggi terdapat pada perlakuan konsentrasi 13 mL/L. Rasio konversi pakan yang rendah (1,08±0,04) juga terdapat pada perlakuan 13 mL/L. Hal ini menunjukkan bahwa perendaman benih ikan lele pada air perasan dapat meningkatkan sistem imun terhadap infeksi bakteri A. hydrophila. Kata kunci: Aeromonas hydrophila, air perasan batang pisang ambon, perendaman, ikan lele, sistem imun

Dietary ambon lumut banana stem extract Musa cavendishii var. dwarf Paxton as an immunostimulant for white spot disease prevention in Pacific white shrimp Litopenaeus vannamei Ramadhan, Afriani; Nuryati, Sri; Priyoutomo, Nur Bambang; Alimuddin, Alimuddin
Jurnal Akuakultur Indonesia Vol. 16 No. 2 (2017): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This study was aimed to evaluate the dietary ambon lumut banana Musa cavendishii var. dwarf Paxton stem extract on the immune responses, growth, and survival rate of Pacific white shrimp against white spot disease. Ambon banana steam extract was obtained by maceration method using ethanol. The shrimps fed by pellet containing ambon banana stem extracts with different dosages for 29 days, i.e designated as 0.1 (A); 0.3 (B), and 0.5 (C) g/kg, the diet treatment without ambon banana stem extract without challenged test (K-), and diet treatment without ambon banana stem extract with challenged test (K+). Each treatment consisted of three replications. Feeding was conducted for 29 days of maintenance (four times a day). The results showed that the immune responses (average total hemocyte count: 45.15×106 cells/mL, phenoloxidase activity 1.03±0.08 OD, respiratory burst 0.95±0.04 OD, phagocytic activity 94.33±1.53%, growth (specific growth rate: 7.79±0.06%/day, feed ratio conversion was 52±0.01), and survival of treatment C (survival rate 100%) were higher compared with the treatment K+ (total hemocyte count: 3.83×106 cells/mL, phenoloxidase activity 0.04±0.01 OD, respiratory burst 0.18±0.06 OD, phagocytic activity 5.67±0.58%, specific growth rate: 2.61±0.08%/day, feed conversion ratio 2.11±0.02, survival rate: 50%). Therefore, banana stem extract at a dose of 0.5 g/kg everyday diet can be used to improve growth and nonspecific immune system against white spot disease on Pacific white shrimp. Keywords: Pacific white shrimp, white spot disease, immune response, Ambon banana stem extract ABSTRAK Penelitian ini bertujuan untuk menguji dosis optimal ekstrak batang pisang ambon melalui pakan dalam meningkatkan respon imun, pertumbuhan dan kelangsungan hidup udang putih terhadap penyakit white spot. Ekstrak batang pisang ambon lumut Musa cavendishii var. dwarf Paxton menggunakan metode maserasi dengan menggunakan pelarut etanol. Pakan udang yang mengandung ekstrak batang pisang ambon pada dosis yang berbeda selama 29 hari 0,1 (A); 0,3 (B), dan 0,5 (C) g/kg, pakan tanpa pemberian ekstrak batang pisang ambon dan tidak diuji tantang (K-), pakan tanpa pemberian ekstrak batang pisang ambon dan diuji tantang (K+). Masing-masing perlakuan terdiri atas tiga ulangan. Frekuensi pemberian pakan empat kali sehari selama 29 hari pemeliharaan. Hasil penelitian menunjukkan bahwa perlakuan C respons imun (rata-rata jumlah total hemosit: 45,15×106 sel/mL, aktivitas phenoloxydase 1,03±0,08 OD, respiratory burst 0,95±0,04 OD, aktivitas fagositik 94,33±1,53%, pertumbuhan (laju pertumbuhan spesifik: 7,79±0,06%/hari, rasio konversi pakan 0,52±0,01), dan kelangsungan hidup (survival rate 100%) lebih tinggi dibandingkan dengan perlakuan K+ (jumlah total hemosit: 3.83×106 sel/mL, aktivitas phenoloxydase 0,04±0,01 OD, respiratory burst 0,18±0,06 OD, aktivitas fagositik 5,67± 0,58%, laju pertumbuhan spesifik: 2,61±0,08%/hari, rasio konversi pakan 2,11±0,02, nilai kelangsungan hidup: 50%). Jadi, ekstrak batang pisang pada dosis 0,5 g/kg setiap hari pada pakan dapat digunakan untuk meningkatkan pertumbuhan dan sistem kekebalan tubuh nonspesifik terhadap penyakit bintik putih pada udang putih. Kata kunci: udang putih, white spot, respons imun, ekstrak batang pisang ambon

The used of chopped banana Musa paradisiaca stem for stimulating immune responses and streptococcosis resistance of Nile tilapia Oreochromis niloticus Nurjanah, Lilis; Nuryati, Sri; Alimuddin, Alimuddin; Nirmala, Kukuh
Jurnal Akuakultur Indonesia Vol. 17 No. 2 (2018): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Streptococcosis caused by Streptococcus agalactiae bacteria, is a type of disease that often found in Nile tilapia farming. This study was performed to determine the effectiveness of the concentration and frequency of giving chopped banana stem as the immunostimulant to stimulate the non‒specific immune system of tilapia against S. agalactiae infection. This study used factorial completely randomized design, consisted of two factors: concentration and changing frequency (replacement time interval) of banana stem on Nile tilapia rearing media with 11 treatment and each treatment was given three replications. The concentration of 5 g/L, 10 g/L and 15 g/L and the three days, seven days and no replacement time interval were used in this study. The immersion treatment with the chopped banana stem was done for 14 days, then the challenge test with S. agalactiae was on the 15th day for 14 days observation. The result showed that chopped banana stem contained active compounds of alkaloids, namely: 17.63% flavonoids, 0.02% tannin and 0.24% saponins. This active compounds could increase the non-specific immune system including respiratory burst, lysozyme activity, phagocyte activity, erythrocyte, leucocyte and haemoglobin. Treatment with a concentration of 5 g/L and no replacement of the chopped banana stem was the best treatment with 75% relative percent survival, highest than other treatments. In addition, it also has highest phagocytes (36.3%), respiratory burst (0.58 at O.D. 630), and lysozyme activity (72.7 unit/mL) after four days challenged with S. agalactiae. Keywords: Banana stem, immunostimulant, Nile tilapia, non-specific immune system, Streptococcus agalatiae ABSTRAK Penyakit Streptococcosis yang disebabkan oleh bakteri Streptococcus agalactiae merupakan jenis penyakit yang sering ditemukan pada budidaya ikan nilaPenelitian ini bertujuan untuk menentukan konsentrasi dan frekuensi pergantian cacahan batang pisang sebagai imunostimulan yang optimum sehingga dapat meningkatkan daya tahan tubuh ikan nila terhadap serangan penyakit streptococcosis yang disebabkan oleh bakteri S. agalactiae. Penelitian ini menggunakan rancangan acak lengkap faktorial. Perlakuan terdiri dari dua faktor yaitu konsentrasi dan frekuensi pergantian cacahan batang pisang pada media pemeliharaan ikan dengan 11 perlakuan dan 3 ulangan. Konsentras batang pisang yang digunakan yaitu 5 g/L, 10 g/L dan 15 g/L, sedangkan frekuensi pergantian batang pisang dalam penelitian ini yaitu tiga hari, tujuh hari dan tanpa pergantian batang pisang. Perlakuan perendaman dengan cacahan batang pisang dilakukan selama 14 hari, kemudian dilakukan uji tantang dengan bakteri S. agalactiae pada hari ke-15 selama 14 hari pengamatan. Hasil penelitian menunjukkan bahwa cacahan batang pisang ambon mengandung senyawa aktif berupa alkaloid, flavonoid (17,63%), tanin (0,02%) dan saponin (0,24%). Senyawa aktif tersebut mampu meningkatkan respons imun non-spesifik pada ikan nila yaitu respiratory burst, aktivitas lisozim, aktivitas fagositik, total leukosit, total eritrosit dan hemoglobin. Perlakuan dengan konsentrasi 5 g/L dan tanpa pergantian cacahan batang pisang merupakan perlakuan terbaik dengan nilai relative percent survival (RPS) mencapai 70%, lebih besar dibandingkan dengan perlakuan lainnya. Selain itu, perlakuan ini memiliki nilai tertinggi pada aktivitas fagositik (36,6%), respiratory burst (0,58 pada O.D. 630 nm) dan aktivitas lisozim (72,7 Unit/mL) setelah hari ke‒4 uji tantang dengan bakteri S. agalactiae. Kata kunci: batang pisang, ikan nila, imunostimulan, respons imun, Streptococcus agalactiae

Efficacy of Streptococcus agalactiae vaccine strains N3M and N4M in fry tilapia infected by different strains of S. agalactiae Sukenda, Sukenda,; Firmansyah, Arif Lukman; Rahman, Rahman,; Nuryati, Sri; Hidayatullah, Dendi
Jurnal Akuakultur Indonesia Vol. 17 No. 2 (2018): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Streptococcus agalactiae is a major bacterial streptococcosis disease that infects tilapia. This study aimed to analyze a specific and nonspecific immune system in fry tilapia that has been given with S. agalactiae vaccine from N3M and N4M strain and examine the protective immunity against S. agalactiae N3M, N4M, N17O, NK1, and N14G strains infection. Fry tilapia used in this study has the weight of 7.086±0.948 g and length of 7.443±0.353 cm. The S. agalactiae strains that used were N3M, N4M, N17O, NK1,and N14G. Fry tilapia was vaccinated through intraperitoneal injection method with 0.1 mL per fish of N3M and N4M vaccines. Fish reared in aquarium sizing of 60×30×50 cm3 with a density of 10 fishes aquarium-1. Two weeks after vaccination, fry tilapia was tested with 0.1 mL fish-1 of each N3M, N4M, N17O, NK1, and N14G strains through intraperitoneal injection method. Antibody level measured with indirect enzym-linked immunosorbent assay (ELISA) method. The result of antibody level in vaccinated fish after vaccination of N3M, N4M vaccine, N3M and N4M control were 0.767; 0.743; 0.587; and 0.544, respectively. Relative percent survival in N3M vaccinated fish after challenged with N3M and N4M was 87.50% dan 64.70%, respectively, otherwise in N4M vaccinated fish was 62.50% dan 76.47%, respectively. N3M and N4M vaccine strain have better protection as only if it tested with similar bacteria strain. Keywords: formalin-killed cell, tilapia, protection, Streptococcus agalactiae, strains ABSTRAK Streptococcus agalactiae merupakan bakteri utama penyakit streptococcosis yang menginfeksi ikan nila. Tujuan penelitian ini adalah menganalisis sistem imun spesifik dan nonspesifik pada benih ikan nila yang diberi vaksin S. agalactiae strain N3M dan N4M serta mengkaji imunitas protektif terhadap infeksi S. agalactiae strain N3M, N4M, N17O, NK1, dan N14G. Benih ikan nila yang digunakan memiliki bobot 7,086±0,948 g dan panjang 7,443±0,353 cm. Bakteri yang digunakan adalah S. agalactiae strain N3M, N4M, N17O, NK1,danN14G. Benih ikan nila divaksinasi menggunakan metode injeksi pada bagian intraperitoneal sebanyak 0,1 mL ekor-1 vaksin N3M dan N4M. Pemeliharaan dilakukan pada akuarium berukuran 60×30×50 cm3 dengan kepadatan 10 ekor akuarium-1. Dua minggu setelah vaksinasi benih ikan diuji tantang dengan strain bakteri N3M, N4M, N17O, NK1, danN14G menggunakan metode injeksi pada bagian intraperitoneal dengan dosis 0,1 mL ekor-1. Level antibodi diukur dengan metode indirect enzym-linked immunosorbent assay (ELISA). Hasil menunjukkan level antibodi ikan setelah vaksinasi pada perlakuan vaksin N3M, N4M, kontrol N3M, dan kontrol N4M berturut-turut 0,767; 0,743; 0,587; dan 0,544. Kelangsungan hidup relatif ikan yang divaksin N3M setelah diuji tantang dengan strain N3M dan N4M berturut-turut 87,50% dan 64,70%, sedangkan pada ikan yang divaksin N4M berturut-turut 62,50% dan 76,47%. Vaksin strain N3M dan N4M memiliki proteksi lebih baik jika diuji tantang dengan strain bakteri yang sama. Kata kunci: formalin-killed cell, Streptococcus agalactiae, strain, proteksi, nila

The use of immunostimulant from phycocyanin of Spirulina platensis to control motile aeromonad septicaemia (MAS) disease in common carp Cyprinus carpio Muchtar, Muthahharah; Sukenda, Sukenda; Nuryati, Sri; Hidayatullah, Dendi
Jurnal Akuakultur Indonesia Vol. 18 No. 1 (2019): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRAK Motile aeromonad septicaemia (MAS) adalah penyakit yang sering menyerang ikan mas Cyprinus carpio yang disebabkan oleh bakteri Aeromonas hydrophila. Penelitian ini bertujuan untuk menguji kinerja imunostimulan fikosianin dari Spirulina platensis dalam mengatasi penyakit MAS pada ikan mas. Penelitian ini terdiri atas dua tahap, pertama, pakan ikan dengan penambahan fikosianin 150 mg/kg, 250 mg/kg, dan 350 mg/kg pakan serta kontrol tanpa penambahan fikosianin. Setelah 14 hari, ikan diuji tantang dengan A.hydrophila. Tahap kedua, dosis terbaik dari penelitian pertama digunakan untuk pakan ikan masing-masing selama satu minggu/bulan, dua minggu/bulan, tiga minggu/bulan, dan dua minggu/bulan dengan interval satu minggu. Setelah 28 hari, ikan diuji tantang dengan A. hydrophila. Hasil penelitian pertama menunjukkan bahwa kelangsungan hidup relatif (RPS) ikan yang diberi pakan fikosianin 150 mg/kg, 250 mg/kg, dan 350 mg/kg pakan adalah 87,50%; 81,25%; dan 75,00%. Total eritrosit, hemoglobin, total leukosit, aktivitas fagositik, dan respiratory burst menunjukkan hasil yang lebih tinggi daripada kontrol untuk semua perlakuan pemberian fikosianin. Penelitian kedua menunjukkan bahwa nilai RPS ikan diberi pakan selama satu minggu/bulan, dua minggu/bulan, tiga minggu/bulan, dan dua minggu/bulan dengan interval satu minggu yaitu 65,38%; 69,23%; 76,92%; dan 69,23%. Respons imun ikan yang diberi fikosianin lebih tinggi daripada kontrol serta mampu menekan jumlah bakteri A. hydrophila di hati, ginjal, dan usus. Kesimpulan dari penelitian ini bahwa pemberian fikosianin sebanyak 150 mg/kg pakan selama tiga minggu/bulan memiliki nilai RPS tertinggi. Kata kunci: fikosianin, Spirulina platensis, Aeromonas hydrophila, Cyprinus carpio ABSTRACT Motile aeromonad septicaemia (MAS) is a major disease in common carp Cyprinus carpio caused by Aeromonas hydrophila. This study aimed to evaluate the performance of phycocyanin imunostimulant extracted from Spirulina platensis to control MAS disease in common carp. This study was conducted into two phases. First phase was conducted by adding 150 mg/kg, 250 mg/kg, 350 mg/kg feed phycocyanin dose, and 0 mg/kg feed phycocyanin dose as control treatment. Fish was challenged with pathogenic A.hydrophila after 14 days rearing. Second phase was conducted by applying the best dose obtained from the first phase added in the feed for feeding the fish in one week/month, two weeks/month, three weeks /month, and two weeks/month with one week interval. Fish was challenged with pathogenic A.hydrophila after 28 days rearing. First phase study result showed that the relative percent survival (RPS) for fish fed 150 mg/kg, 250 mg/kg, and 350 mg/kg phycocyanin dose were 87.50%, 81.25%, and 75.00% respectively. Total erythrocytes, hemoglobin, total leucocytes, phagocytic activity, and respiratory burst showed higher results than control treatment on all treated fish. The second phase study showed that fish fed one week/month, two weeks/month, three weeks/month, and two weeks/month with one week interval had RPS value 65.38%, 69.23%, 76.92%, and 69.23% respectively. The immune responses of treated fish were higher than control treatment, as well as the number of pathogenic A. hydrophila in the liver, kidney, and intestine. Fish fed with phycoyanin dose 150 mg/kg feed and three weeks/month administration had the highest RPS value. Keywords: Phycocyanin, Spirulina platensis, Aeromonas hydrophila, Cyprinus carpio

Identification and expression analysis of c-type and g-type lysozymes genes after Aeromonas hydrophila infection in African catfish Nasrullah, Hasan; Nababan, Yanti Inneke; Yanti, Dwi Hany; Hardiantho, Dian; Nuryati, Sri; Junior, Muhammad Zairin; Ekasari, Julie; Alimuddin, Alimuddin
Jurnal Akuakultur Indonesia Vol. 18 No. 2 (2019): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTLysozymes play an important role in the first line of defense in fish and potentially used as an immunity status biomarker and immune responses evaluation in fish, which often found in two types, i.e. chicken-type and goose-type (c- and g-type, respectively). To recent, the information related to the sequences and the expression analysis of the c- and g-type lysozyme genes in African catfish is still limited. In the present study, we report a partial cloning and mRNA expression analysis of c-type and g-type lysozymes in African catfish Clarias gariepinus. We have successfully cloned and partially identify the c-type, and g-type lysozyme genes of C. gariepinus, which consist of 594 and 560 of coding sequences, respectively. Catalytic and other conserved residues were identified by multiple sequences alignment and they showed high similarity with other teleost fish species. mRNA levels of the genes were analyzed by using qPCR method and their expressions in the spleen, liver, and head kidney were rapidly modulated after Aeromonas hydrophila injection, with different patterns were observed in each organ. These results confirmed that c- and g-type lysozymes played an important role in non-specific immunity against A. hydrophila infection. This study provided valuable information that can be used to understand the African catfish immune systems for better disease and stress management in C. gariepinus culture.Keywords: lysozymes, gene identification, gene expression, bacterial infection, African catfish ABSTRAKLisozim berperan dalam sistem pertahanan dini pada ikan dan sangat potensial digunakan sebagai marka status imunitas dalam evaluasi respons imun. Lisozim umum ditemukan dalam dua tipe pada ikan: tipe-ayam (tipe-c) dan tipe-angsa (tipe-g). Informasi terkait sekuens gen dan ekspresi gen kedua tipe lisozim pada ikan lele dumbo sangat terbatas. Pada penelitian ini, kami melaporkan kloning gen secara parsial, dan analisis ekspresi gen dari kedua tipe lisozim pada ikan lele dumbo C. gariepinus. Sekuens parsial gen lisozim tipe-c dan tipe-g yang berhasil diidentifikasi adalah sepanjang 594 dan 560 pasang basa. Situs katalitik dan residu khas memiliki tingkat kesamaan yang tinggi dengan spesies ikan yang lain. Analisis mRNA dilakukan dengan metode quantitative PCR (qPCR). Ekspresi kedua gen di jaringan ginjal depan, limpa, dan hati dengan cepat terinduksi pasca infeksi bakteri A. hydrophila dengan pola yang berbeda. Hasil ini menunjukkan bahwa lisozim tipe-c dan tipe-g memiliki peran yang sangat penting dalam sistem imun ikan lele dumbo terhadap infeksi A. hydrophila. Penelitian ini menghasilkan informasi penting yang dapat digunakan untuk mempelajari sistem imun ikan lele dumbo dan sebagai acuan dalam penanganan dan manajemen penyakit pada budidaya ikan lele dumbo.Kata kunci: lisozim, identifikasi gen, ekspresi gen, infeksi bakteri, ikan lele dumbo

Co-Authors , Alimuddin , Rahman, , , Rusli, , , Sofian, , , Yuliyanti . Kurdianto . Taukhid A. Santika A. Suwanto Ade Sunarma Aditya, Tiya Widi Adna Sumadikarta Afif Abdurrahman Afiff , Usamah Agung Cahyo Setyawan Agus Oman Sudrajat Ahmad Beni Rouf Ahmad Muzaki Alimuddin Alimuddin Alimuddin Alimuddin Andi Tiara Eka Diana Puteri, Andi Tiara Eka Diana Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Anis Nugrahawati Anna Octavera Ardana Kurniaji Asrido, Farhan Ayi Santika Ayi Santika Ayu Dhita Juliadiningtyas, Ayu Dhita Bagus Rahmat Basuki Brite, Margie D. Meha D. Puspitaningtyas D. Wahjuningrum DEDI JUSADI Dendi Hidayatullah Dendi Hidayatullah Dendi Hidayatullah Dendi Hidayatullah, Dendi Dian Febriani Dian Hardiantho Dian Hardiantho Dinamella Wahjuningrum Dinar Tri Soelistyowati Dwi Hany Yanti Dwi Hany Yanti Dwi Yanti Eddy Supriyono Eni Kusrini Eni Kusrini Erni Susanti F.B.P. Sari F.H. Pasaribu Fachriyan Hasmi Pasaribu Fadhila Maharani Putri Fajrin, Anang Faoziyatunnisa, Nurul Fauzan Wahib Alsani Firdausi, Amalia Putri Firmansyah, Arif Lukman Fitriyah Husnul Khotimah Goro Yoshizaki Gusti Ngurah Permana Hanggara, Yudha Harits Atika Ariyatna Harton Arfah Haryanti Haryanti Haryanti Haryanti Hasan Nasrullah Hasan Nasrullah Hidayah, Winda Alfina Ibnu Bangkit Bioshina Suryadi Iis Widiani Ika Safitri Ikhsan Khasani Indah Mastuti Irzal Effendi ISNAWATI Isni Rahmatika Sari Julie Ekasari K. Sumantadinata Ketut Mahardika Ketut Sugama Ketut Sugama Khotimah, Fitriyah Husnul Komar Sumantadinata Kukuh Nirmala Kurnia Anggraini Rahmi L.S. Harti La Ode Muhammad Arsal Lili Sholihah Lilis Nurjanah M. Zairin Junior M.A. Suparman Mia Setiawati Min Rahminiwati Mira Mawardi Muchtar, Muthahharah MUHAMMAD AGUS SUPRAYUDI Muhammad Zairin Jr Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. Mulyani, Rahma MUNTI YUHANA N. Ashry N.A. Maswan Nadia Aulia, Nadia Nasrullah, Hasan Nurbariah Nurbariah Nurina Pratiwi Nurly Faridah Nurly Faridah NURWATI, BUNGA Odang Carman P. Giri P. Hariyadi Priyoutomo, Nur Bambang Putri, Elva Khairina Qorie Astria R.D. Soejoedono Rahma Mulyani Rahman Ramadhan, Afriani Ramadhani, Danika Athirah Ramadhina, Erina Tri Retno Damayanti Soejoedono Rizkiyanti, Ita S.H. Sholeh Sari Anggraeni, Sukma Sari Budi Moria Sembiring Sari Budi Moria Sembiring Sarjito - SATA YOSHIDA SRIE RAHAYU Sekar Ayu Chairunnisa Sekar Ayu Chairunnisa Sekar Sulistyaning Hadiwibowo Shavika Miranti Shella Marlinda Siti Khodijah Siti Murniasih Siti Zubaidah Slamet Budi Prayitno Soko Nuswantoro Sri Murtini Suhermanto, Achmad Sukenda Sukenda Sukenda, Sukenda, Sulasy Rohmy Taufiq Abdullah, Taufiq Tiara Puspa Anjani Tira Silvianti Titin Kurniasih Trian Rizky Febriansyah Tuti Sumiati Tuti Sumiati Utomo, Nurbambang Priyo Uttari Dewi Wahyuwardani , Sutiastuti Wasmen Manalu Wida Lesmanawati WIDANARNI WIDANARNI Y. Hadiroseyani Y. Kuswardani Yanti Inneke Nababan Yanti, Mauliza Yulianti, Susy Anisa Yuni Puji Hastuti Zakki Zainun Zulhelmi, Arif

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