Garuda - Garba Rujukan Digital

p-Index From 2021 - 2026

4.221

P-Index

This Author published in this journals

All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Jurnal Perikanan Kelautan Jurnal Natur Indonesia Journal of Aquaculture Management and Technology BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Ilmu-Ilmu Perikanan dan Budidaya Perairan Omni-Akuatika Jurnal Perikanan Universitas Gadjah Mada Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Journal of Aquaculture and Fish Health Jurnal Abdi Insani Jurnal Kedokteran Hewan Journal of Fisheries & Marine Biodiversitas Journal of Biological Diversity

Sri Nuryati

Department Of Aquaculture, Faculty Of Fisheries And Marine Sciences, IPB University Bogor, West Java, 16680. Indonesia

Author-ID : 336092

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Veterinary Other

Published : 77 Documents Claim Missing Document

Claim Missing Document

Articles

3 4 5 6 7

Efficacy of DNA vaccine encoding koi herpesvirus glycoprotein GP-25in common carp juvenile by immersion Nuswantoro, Soko; Alimuddin, .; Yuhana, Munti; Santika, Ayi; Nuryati, Sri; Zainun, Zakki; Mawardi, Mira
Jurnal Akuakultur Indonesia Vol. 11 No. 1 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Koi herpesvirus (KHV) is a herpesvirus that particularly infects and causes mass mortality to koi and common carp. Therefore, the protection of common carp from KHV infection is urgently needed. In this study, we developed an application of DNA vaccine encoding KHV glycoprotein-25 by immersion method to increase survival of common carp against KHV infection. A total of 400 common carp juveniles at 30-day-old were immersed in 1-L water containing 1.3×108CFU/mL of the killed Escherichia coli cells carrying DNA vaccine. Three frequencies and three duration of fish immersion were tested, namely: 1×30 minutes, 1×60 minutes, 1× 90 minutes, 2×90 minutes and 3×90 minutes by interval of 24 hours. Reversetranscription polymerase chain reaction analysis showed that DNA vaccine was successfully expressed in the vaccinated fish. Fish at twenty eight days post vaccination were challenged by injecting 10-4 mL of KHV per fish. The result showed that vaccination by 1×30 minutes immersion allowed 61% of fish survived, and this was significantly higher (p<0.05) compared to control (without vaccination), but it was similar among vaccination treatments (p>0.05). The relative percent survival of vaccinated fish were also similar among treatments (p>0.05). DNA vaccination has increased fish survival about two fold higher compared to unvaccinated fish control (26.67%). Thus, DNA vaccination was effectively delivered by immersion for 1×30 minutes, and this technique can be useful to level up the resistance of common carp juveniles against KHV infection. Keywords: DNA vaccine, KHV, glycoprotein, immersion, common carp

Duration of feed application of mixed powders of Phyllanthus niruri and Allium sativum for the prevention of Aeromonas hydrophila infection in catfish Clarias sp. Wahjuningrum, Dinamella; Widiani, Iis; Nuryati, Sri
Jurnal Akuakultur Indonesia Vol. 11 No. 2 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Motile Aeromonad Septicaemia (MAS) caused by Aeromonas hydrophila induced serious epidemics of disease in catfish Clarias sp. The purposed of this research was to determine optimum durations of feed application of mixed powders Phyllanthus niruri and Allium sativum to prevent MAS. Experimental design that used was Complete Random Design which consist of three treatments and three replications. This research tested on three different of durations of feed application of P. niruri and A. sativum, namely A (powder of P. niruri and A. sativum feed application during 21 days and infected with A. hydrophila 0,1 mL IP), B (powder of P. niruri and A. sativum feed application during 14 days and injected IP with A. hydrophila 0,1 mL), C (powder of P. niruri and A. sativum feed application during seven days and injected IP with A. hydrophila 0,1 mL), and control, namely K- (without P. niruri and A. sativum feed application and injected IP with PBS 0,1 mL) and K+ (without P. niruri and A. sativum powder application and injected IP with A. hydrophila 0,1 mL). The treatments was given for 21 days before challenging test, at 22th day test in vivo carried out by injecting A. hydrophila (108 CFU/mL) into the fish by intramuscular and observed for 10 days. The highest value of survival rate that consist in treatment K- was 100±0.00%, treatment A was 93.3±11.55%, and treatment B was 73.33±30.55%. While treatment K+ and C have the same survival rate that is, 26.67±11.55%. The application P. niruri and A. sativum powder during 21 days was optimum for preventing MAS in catfish.Keywords: Phyllanthus niruri, Allium sativum, Aeromonas hydrophila, Clarias sp.

Artemia sp. as a DNA vaccine vector for common carp Cyprinus carpio larvae Nuryati, Sri; Hadiwibowo, Sekar Sulistyaning; Alimuddin, .
Jurnal Akuakultur Indonesia Vol. 12 No. 1 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Koi herpes virus (KHV) is one of the most common impetuses for disease on common carp Cyprinus carpio. Generally, viral disease is difficult to cure because virus is intra-cellular parasite, that virus survives, multiplies, and lives only if it on the host cell. Oral vaccine delivery through Artemia sp. is of one alternative way to overcome this problem. This experiment was carried out by analysis DNA vaccine expression encoding of glycoprotein gene (GP-11) on C. carpio. Bacteria containing plasmid Krt-GP-11 as vaccine is served through Artemia sp. as a vector. Artemia sp. was given for one and two times a week to three weeks old common carp. Organs of fish fed by Artemia sp. were analyzed every three days after vaccination. The expression of GP-11 in kidney in each treatment is also observed by the use of RT-PCR method, within ten days after vaccination. The experiment showed that dose of DNA vaccine in whole bacteria could be expressed is 106 cfu/mL in a once or twice provisions a week. DNA vaccine could be detected in three organs. RT-PCR analysis also showed that the expression of GP-11 can be detected in all tested organs. In conclusion, Artemia sp. can be used as a vector to carry plasmid GP-11 vaccine for common carp Cyprinus carpio larvae. Keywords: DNA vaccine, KHV, Artemia sp., common carp ABSTRAK Salah satu penyakit pada ikan mas (Cyprinus carpio) yang disebabkan oleh virus adalah koi herpes virus (KHV). Penyakit yang disebabkan oleh virus umumnya sulit untuk disembuhkan karena virus merupakan parasit intraseluler, yaitu virus hanya dapat hidup, bertahan hidup, dan memperbanyak diri di dalam sel inang. Metode pemberian vaksin DNA secara oral melalui Artemia sp. merupakan salah satu alternatif pengobatan yang diharapkan dapat menangani permasalahan penyakit pada ikan yang disebabkan oleh virus. Pada penelitian ini dilakukan uji ekspresi vaksin DNA yang menyandikan glikoprotein 11 (GP-11) pada ikan mas. Bakteri yang mengandung plasmid Krt-GP-11 sebagai vaksin diberikan melalui Artemia sp. sebagai pembawa vaksin. Pemberian Artemia sp. dilakukan satu dan dua kali seminggu pada ikan mas umur tiga minggu. Keberadaan DNA vaksin di usus, ginjal, dan insang dianalisis menggunakan metode PCR. Organ diambil setiap tiga hari setelah pemberian vaksin. Ekspresi gen GP-11 juga diamati pada organ ginjal di setiap perlakuan dengan menggunakan metode RT-PCR, pada sepuluh hari setelah pemberian vaksin. Hasil penelitian menunjukkan bahwa DNA vaksin yang diberikan dengan dosis 106 cfu/mL pada perlakuan satu dan dua kali seminggu dapat terdeteksi pada ketiga organ. Hasil RT-PCR menunjukkan bahwa ekspresi GP-11 dapat terdeteksi pada semua organ uji di setiap perlakuan. Dengan demikian Artemia sp. dapat digunakan sebagai vektor pembawa vaksin plasmid GP-11 dengan frekuensi pemberian vaksin untuk larva ikan mas. Kata kunci: vaksin DNA, KHV, Artemia sp., ikan mas

Kappa-carrageenan as immunostimulant to control infectious myonecrosis (IMN) disease in white shrimp Litopenaeus vannamei Febriani, Dian; Sukenda, .; Nuryati, Sri
Jurnal Akuakultur Indonesia Vol. 12 No. 1 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This study evaluated the modulation of non-specific immune response, growth, and disease resistance of white shrimp, Litopenaeus vannamei against infectious myonecrosis virus (IMNV). The first stage of this study evaluated the different dose of k-carrageenan administration i.e. 5, 10, and 15 g/kg feed for four weeks of rearing period, while the later studied evaluated about the frequency of administration i.e. daily, seven days interval, and 14-days interval for five weeks of rearing period. Both stages had positive and negative control and performed in complete randomized design. The parameters of observation consisted of immune parameters, clinical symptoms, growth, and survival. Shrimp were fed three times a day at feeding rate of 4–5% of body weight/day. IMNV Challenge test was performed by feeding the shrimp via oral route at a level 10% of body weight for three consecutive days, followed by 14-days observation. The results showed that shrimp administered with k-carrageenan at a concentration of 15 g/kg feed showed the best performance of all concentration tested. The shrimp’s haemocyte count, phagocytes activity, phenoloxidase activity, and relative growth were 12±0.72×106 cell/mL; 34.67±0.58%; 0.511±0.10; and 86.15% respectively. After challenged, the survival was 85±7.07%. Moreover, application in 14 days at 7-days interval gave 88.57% relative growth and 93±5,8% survival, which were higher than other treatments. The administration of k-carrageenan at concentration of 15 g/kg with 14 days interval on white shrimp juveniles showed higher immunostimulatory effect and better protection against IMNV. Keywords: kappa-carrageenan, immunostimulant, IMNV, Litopenaeus vannamei ABSTRAK Penelitian ini mempelajari pemberian kappa-karagenan dalam memodulasi respons imun nonspesifik, pertumbuhan, dan resistensi udang vaname Litopenaeus vannamei terhadap infeksi infectious myonecrosis virus (IMNV). Tahap pertama mengetahui dosis pemberian k-karagenan sebesar 5, 10, dan 15 g/kg pakan selama empat minggu pemeliharaan, sedangkan tahap kedua mengevaluasi frekuensi pemberian k-karagenan, yaitu setiap hari, tujuh hari, dan 14 hari secara berulang dengan interval tujuh hari selama lima minggu pemeliharaan. Kedua tahap penelitian menggunakan kontrol positif dan negatif dalam rancangan acak lengkap. Parameter pengamatan terdiri atas respons imun, gejala klinis, pertumbuhan dan kelangsungan hidup udang vaname. Udang diberi makan tiga kali sehari dengan FR 4–5% biomassa/hari. Infeksi IMNV dilakukan secara oral sebesar 10% biomassa selama tiga hari berturut-turut, dan diamati selama 14 hari. Hasil penelitian menunjukkan bahwa udang yang diberikan k-karagenan 15 g/kg pakan memperoleh hasil terbaik. Total hemosit, aktivitas fagositosis, aktivitas phenoloxidase, dan pertumbuhan relatif udang masing-masing adalah 12±0,72×106 sel/mL; 34,67±0,58%; 0,511±0,10 dan 86,15%, dengan kelangsungan hidup udang setelah diinfeksi IMNV sebesar 85±7,07%. Frekuensi pemberian 14 hari secara berulang dengan interval tujuh hari memberikan hasil kelangsungan hidup terbaik sebesar 88,57% dan pertumbuhan relatif sebesar 93±5,8%. Kesimpulan dari penelitian ini bahwa, pemberian k-karagenan 15 g/kg dan interval 14 hari menunjukkan respons imun dan perlindungan yang lebih baik terhadap IMNV. Kata kunci: kappa-karagenan, imunostimulan, IMNV, Litopenaeus vannamei

Administration of Phyllanthus niruri to control IMNV (myonecrosis infectious virus) infection white shrimp Litopenaeus vannamei ., Sukenda; Nuryati, Sri; Sari, Isni Rahmatika
Jurnal Akuakultur Indonesia Vol. 10 No. 2 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTInfectious myonecrosis (IMN) disease is a major disease in Indonesia shrimp farming. The disease is caused by infectious myonecrosis virus (IMNV). Currently, treatment and drug has not been obtained to control the virus. This research was conducted to determine the effect of Phyllanthus niruri extract in white shrimp (Litopenaeus vannamei) against IMNV infection. Healthy shrimp was given P. niruri extract 20 mg/kg of feed for seven days and after that the shrimp was challenged by orally with IMNV infected shrimp tissue. The positive control was given feed without P. niruri extract and challenged with IMNV infected shrimp tissue, while negative control was not challenged with IMNV infected shrimp tissue. IMNV infection gave a significantly different effect on survival rate. In the shrimp P. niruri previously (86.7%) gave higher survival rate compared to shrimp without P. niruri (66.67%). Survival rate of negative control was 93.33%. IMNV clinical signs in general was white necrotic areas in striated muscles. Histological examination showed that cell necrosis appeared on the mussel tissues. In conclusion the addition of P. niruri to the commercial feed can give the survival rate of shrimp better when challenged with IMNV.Keywords: IMNV, Phyllanthus niruri, Litopenaeus vannameiABSTRAKPenyakit infectious myonecrosis (IMN) merupakan penyakit utama pada budidaya udang di Indonesia. Penyakit ini disebabkan oleh infectious myonecrosis virus (IMNV). Saat ini, belum diperoleh cara dan obat untuk mengendalikan virus IMNV. Penelitian ini dilakukan untuk mengevaluasi pengaruh immunostimulan tepung meniran (Phyllanthus niruri) yang diberikan melalui pakan pada udang vaname (Litopenaeus vannamei) yang diinfeksi IMNV. Udang vaname yang sehat diberi pakan yang mengandung meniran dengan dosis 20 mg/kg pakan selama tujuh hari dan kemudian diuji tantang secara oral dengan memberikan jaringan udang yang telah terinfeksi IMNV. Udang kontrol positif dilakukan dengan memberi pakan komersial tanpa penambahan meniran yang kemudian diuji tantang dengan memberi makan jaringan udang yang terinfeksi IMNV, sedangkan udang kontrol negatif tidak diuji tantang dengan jaringan yang terinfeksi IMNV. Hasil menunjukkan bahwa kelompok udang yang diberi pakan mengandung meniran mempunyai kelangsungan hidup (86,67%) lebih tinggi dibandingkan dengan udang yang tidak diberi pakan mengandung meniran (66,67%) ketika diuji tantang dengan IMNV. Kontrol negatif yang tidak diberi pakan mengandung meniran dan tidak diuji tantang dengan IMNV memberikan kelangsungan hidup 93,33%. Gejala klinis yang ditunjukkan adanya infeksi IMNV terlihat dengan adanya otot putih pada ruas tubuh udang. Pengamatan histopatologi menunjukkan adanya nekrosis pada sel-sel di jaringan otot udang. Sebagai kesimpulan dapat dinyatakan bahwa penambahan meniran pada pakan komersial dapat meningkatkan kelangsungan hidup udang ketika terjadi infeksi IMNV.Kata kunci: IMNV, Phyllanthus niruri, Litopenaeus vannamei

Frequency and persistency of DNA vaccine encoding GP25 by oral on common carp Nuryati, Sri; Yuliyanti, ,; Alimuddin, ,
Jurnal Akuakultur Indonesia Vol. 12 No. 2 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Koi herpesvirus (KHV) is a major viral pathogen that infects common carp and koi. KHV disease outbreak is happened in almost all centre of common carp culture in Indonesia and caused mass mortality. Deoxyribonucleic acid (DNA) vaccination method is one of ways to cope with KHV infection. Vaccines were commonly given by injection. The aim of this research was to get frequency and persistency of DNA vaccine encoding GP25 given by oral delivery method in common carp. This research would like to determine dose, frequency of vaccination, persistency of DNA vaccine and culture medium for the bacterial host. DNA vaccine persistency test was done by using polymerase chain reaction (PCR) method with the specific primer for GP25 gene. The results showed that level of DNA vaccine that could be detected in feed was 7.56 ng (equal to 1.598×1010 copies). Efficient culture medium for Escherichia coli DH5α carrying DNA vaccine was LB triptone. Feeding fish with diet supplemented with 1 mL E. coli DH5α containing DNA vaccine for each fish and two times a week allowed persistence of DNA vaccine in kindney and spleen. Keywords: common carp, KHV, DNA vaccine, GP25, persistance ABSTRAK Koi herpesvirus (KHV) adalah virus patogen utama yang menginfeksi ikan mas dan ikan koi. Wabah penyakit KHV terjadi di hampir semua sentra budidaya ikan mas di Indonesia dan menyebabkan kematian massal ikan. Metode vaksinasi DNA merupakan salah satu cara yang dapat dilakukan untuk menanggulangi serangan KHV. Pemberian vaksin umumnya dilakukan dengan cara injeksi. Tujuan penelitian ini adalah untuk menguji frekuensi dan persistensi vaksin DNA GP25 antivirus KHV yang diberikan melalui oral pada ikan mas. Pada penelitian ini dilakukan uji dosis, frekuensi pemberian vaksin, persistensi vaksin DNA, dan media kultur bakteri inang. Persistensi vaksin DNA dianalisis menggunakan metode PCR dengan primer spesifik gen GP25. Hasil penelitian menunjukkan bahwa dosis vaksin DNA yang dapat terdeteksi dalam pakan adalah 7,56 ng (setara dengan 1,598×1010 copy). Media kultur yang efisien bagi bakteri Escherichia coli DH5α pembawa vaksin DNA adalah LB tripton. Pemberian pakan bervaksin berupa bakteri konsentrasi 1 mL/ekor ikan dengan frekuensi dua kali seminggu menghasilkan persistensi DNA GP25 di ginjal dan limpa. Kata kunci: ikan mas, KHV, vaksin DNA, GP25, persistensi

Whole-cell vaccine of Streptococcus agalactiae in Oreochromis sp. with immersion method Sukenda, ,; Febriansyah, Trian Rizky; Nuryati, Sri
Jurnal Akuakultur Indonesia Vol. 13 No. 1 (2014): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The study was aimed to evaluate the efficacy of formalin-killed non-hemolytic Streptococcus agalactiae N14G and NK1 isolates whole-killed vaccine to prevent streptococcosis in tilapia. Ten fishes were reared in a tank 60x30x35 cm3 with an average body weight at 10.79±0.99 g. Fish was vaccinated through bath immersion at a concentration of 109 cfu/mL. Fish was subsequently challenged by intraperitonial injection of Streptococcus agalactiae 105 cfu/mL at 11 days post-vaccination. Parameters observed were survival, relative percent survival (RPS), total leukocyte, phagocytic activity, antibody titer, total erythrocyte, haemoglobin level, haematocrit level, dan water quality. Samplings were performed in day-0, 20, and 30 after vaccination. Both vaccines have shown higher survival (60%) and RPS (40%) when challenged with pathogenic Streptococcus N14G isolates than other treatments. Based on RPS percentage observed, those vaccine were still not sufficiently effective to combat S. agalactiae infection. Keywords: tilapia, bath immersion, Streptococcus agalactiae, whole-cell vaccine ABSTRAK Penelitian ini bertujuan untuk mengevaluasi efikasi vaksin formalin-killed cell Streptococcus agalactiae tipe isolat nonhemolitik N14G dan NK1 se utuh yang diberikan melalui perendaman dalam mencegah penyakit streptococcosis pada ikan nila. Ikan nila yang digunakan memiliki bobot 10,79±0,99 g, dipelihara sebanyak sepuluh ekor dalam akuarium ukuran 60x30x35 cm3. Ikan divaksinasi dengan metode perendaman dengan dosis 109 cfu/mL. Uji tantang dilakukan pada hari ke-11 pascavaksinasi dengan dosis 105 cfu/mL. Parameter yang diamati meliputi sintasan (SR), sintasan relatif/relative percent survival (RPS), total leukosit, aktivitas fagositik, titer antibodi, total eritrosit, kadar hemoglobin, kadar hematokrit, dan kualitas air. Pengamatan parameter dilakukan pada hari ke-0, ke-10, ke-20, dan ke-30. Hasil penelitian menunjukkan perlakuan kedua vaksin yang diinfeksi bakteri patogen isolat N14G memberikan nilai sintasan dan nilai RPS tertinggi dibanding perlakuan lainnya. Nilai sintasan dan RPS kedua perlakuan tersebut adalah 60% dan 40%. Nilai RPS yang cukup kecil menunjukkan vaksin yang diberikan masih kurang efektif untuk mencegah infeksi bakteri S. agalactiae. Kata kunci: ikan nila, perendaman, Streptococcus agalactiae, vaksin sel utuh

Survival of common carp carrying Cyca-DAB1*05 post-challenged with Aeromonas hydrophila Arsal, Laode Muhammad; Yuhana, Munti; Nuryati, Sri; Alimuddin, ,
Jurnal Akuakultur Indonesia Vol. 13 No. 2 (2014): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Blood parameters are considered as important indicators to diagnose fish health status. This study was performed to observe blood profiles including total erythrocytes, hemoglobin concentration, hematocrite, total leukocytes and differential leukocytes, and survival of common carp Cyprinus carpio infected by Aeromonas hydrophila. Fish were divided into two groups: the 3rd generation of common carp carrying fish carrying Cyca-DAB1*05 of major histocompatibility complex II molecular marker, and fish without the marker as control treatment. histocompatibility complex II molecular marker, and fish without the marker as control treatment. Common carp 3rd generation was produced by crossing among second generation of fish carrying the Cyca-DAB1*05 marker. Each fish was injected intramuscularly by 0.1 mL of 108 cfu/mL A. hydrophila. Challenge test was conducted for 14 days and blood was collected at day-0, three, seven, and 14. The results of this study showed that erythrocytes, hemoglobin and hematocrite concentrations of common carp carrying the molecular marker at post challenge with A. hydrophila were higher (P<0.05) compared to control fish. The blood profiles were highly correlated to survival of fish. Survival of fish that carrying the molekuler marker was about two point six fold higher than those of control fish. Keywords: Cyprinus carpio, Aeromonas hydrophila, Cyca-DAB1*05, molecular marker ABSTRAK Gambaran darah merupakan indikator penting untuk mendiagnosa penyakit ikan. Penelitian ini dilakukan untuk meguji gambaran darah ikan mas Cyprinus carpio setelah diinfeksi dengan bakteri Aeromonas hydrophila yang meliputi total sel darah merah, konsentrasi hemoglobin, hematokrit, total sel darah putih, dan diferensial leukosit, serta sintasannya. Ikan mas yang digunakan terdiri atas ikan mas generasi ketiga yang membawa marka molekuler Cyca-DAB1*05 dari kelompok major histocompatibility complex II dan ikan mas tanpa marka sebagai kontrol. Ikan mas generasi ketiga merupakan keturunan persilangan antarikan mas generasi kedua yang mempunyai marka Cyca-DAB1*05. Masing-masing ikan diinfeksikan A. hydrophila secara intramuskuler pada dosis 0,1 mL, kepadatan 108 cfu/mL. Uji tantang dilakukan selama 14 hari, dan sampel darah ikan diambil pada hari ke-0, tiga, tujuh dan 14. Hasil uji tantang menunjukkan bahwa total sel darah merah, konsentrasi hemoglobin dan hematokrit pada ikan mas yang membawa marka molekuler lebih tinggi dibandingkan ikan mas tanpa marka (P<0,05). Hasil uji gambaran darah berkorelasi tinggi dengan tingkat kelangsungan hidup ikan mas. Tingkat kelangsungan hidup ikan mas yang membawa marka molekuler Cyca-DAB1*05 lebih tinggi hingga dua koma enam kali daripada ikan mas kontrol. Kata kunci: Cyprinus carpio, Aeromonas hydrophila, Cyca-DAB1*05, marka molekuler

Potential transmission test of GP25 vaccine in normal flora bacteria of common carp culture media Nuryati, Sri; Alimuddin, ,; Juliadiningtyas, Ayu Dhita
Jurnal Akuakultur Indonesia Vol. 14 No. 1 (2015): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Koi herpesvirus (KHV) is a virus that infects common carp and koi Cyprinus carpio. KHV attacks most stadia of common carp and koi and causes mortality up to 80–95% of the population. One way to prevent the spread of KHV is by applying DNA vaccine. This research was conducted to test potential transmission of DNA vaccine encoding glycoprotein 25 (vaccine GP25) toward normal flora bacteria of media cultivation of common carp. Bacteria was isolated from pond water of common carp and tested for their sensitivity to ampicillin. Research was performed by adding vaccine GP25 to ampicillin-sensitive bacteria at a dose of 12.5 µg/100 µL and incubated at 28 °C for 30, 60, 180, and 300 minutes then plated on media containing ampicillin. The grown bacteria cells were tested for the existence of plasmid bearing gen GP25 through bacteria colony cracking. The results of this research showed that there was no bacteria contained plasmid bearing gen GP25. Keywords: DNA vaccines, common carp, vaccine safety ABSTRAK Koi herpesvirus (KHV) merupakan virus yang menginfeksi ikan mas dan koi Cyprinus carpio. KHV menyerang hampir semua stadia ikan mas dan koi dan menyebabkan kematian hingga 80–95% dari populasi. Salah satu cara penanggulangan penyebaran KHV adalah dengan penggunaan vaksin DNA. Penelitian ini dilakukan untuk menguji potensi transmisi vaksin DNA glikoprotein 25 (vaksin GP25) pada bakteri flora normal pada media budidaya ikan mas. Bakteri diisolasi dari kolam budidaya ikan mas dan diuji sensitivitasnya terhadap antibiotik ampisilin. Penelitian dilakukan dengan menambahkan vaksin GP25 ke larutan bakteri sensitif ampisilin dengan dosis 12,5 µg/100 µL dan diinkubasi pada suhu 28 °C selama 30, 60, 180, dan 300 menit kemudian disebar pada media mengandung antibiotik ampisilin. Sel bakteri yang tumbuh diuji untuk mendeteksi keberadaan plasmid pembawa gen GP25 dengan menggunakan metode seleksi koloni bakteri (colony cracking). Hasil penelitian ini menunjukkan bahwa tidak ada bakteri yang mengandung plasmid pembawa gen GP25. Kata kunci: keamanan vaksin, ikan mas, vaksin DNA

Effectivity of Musa paradisiaca extract to control Saprolegnia sp. infection on giant gourami larvae Nuryati, Sri; Aulia, Nadia; Rahman, ,
Jurnal Akuakultur Indonesia Vol. 14 No. 2 (2015): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Larval stage of giant gourami is a critical period due to fungal infection, such as Saprolegnia sp. infection. There are some plants which have antiseptic compound like banana Musa paradisiaca. This research was aimed to examine the effectiveness of the banana stem extract M. paradisiaca to control Saprolegnia sp. infection on giant gurami larvae through immersion. Eight-day old gorami larvae (at the initial of 0.5±0.03 cm) was reared in an aquarium sized 25×25×25 cm3 at the density of 8 fry/L. Culture media were added banana stem extract at the dose of 0; 0.08; 0.12; and 0.16 g/L during 21 days of rearing period. Challenge test was performed for 14 days by giving Saprolegnia sp. spores at the density of 104 cells/mL and banana stem extract. The treatment dose of 0.16 g/L has showen survival 100% than positive control after the challenge test. Keywords: giant gourami, Musa paradisiaca, Saprolegnia sp., fry ABSTRAK Fase larva ikan gurami merupakan masa kritis terhadap infeksi cendawan, seperti jenis Saprolegnia sp. Beberapa tanaman memiliki daya antiseptik seperti tanaman pisang ambon Musa paradisiaca. Penelitian ini bertujuan untuk menguji efektivitas ekstrak batang pisang ambon M. paradisiaca dalam mengurangi infeksi Saprolegnia sp. pada larva ikan gurami melalui media pemeliharaan. Larva gurami umur delapan hari (panjang larva 0,5+0,03 cm) dipelihara pada akuarium berukuran 25×25×25 cm3 dengan padat tebar 8 ekor/L. Media pemeliharaan diberi ekstrak batang pisang ambon dosis 0; 0,08; 0,12; dan 0,16 g/L selama 21 hari. Uji tantang dilakukan selama 14 hari dengan pemberian spora Saprolegnia sp. kepadatan 104 sel/mL dan ekstrak batang pisang ambon. Perlakuan dosis 0,16 g/L memberikan kelangsungan hidup sebesar 100% yang lebih tinggi dibandingkan perlakuan kontrol positif setelah uji tantang. Kata kunci: giant gourami, Musa paradisiaca, Saprolegnia sp., larva

Co-Authors , Alimuddin , Rahman, , , Rusli, , , Sofian, , , Yuliyanti . Kurdianto . Taukhid A. Santika A. Suwanto Ade Sunarma Aditya, Tiya Widi Adna Sumadikarta Afif Abdurrahman Afiff , Usamah Agung Cahyo Setyawan Agus Oman Sudrajat Ahmad Beni Rouf Ahmad Muzaki Alimuddin Alimuddin Alimuddin Alimuddin Andi Tiara Eka Diana Puteri, Andi Tiara Eka Diana Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Anis Nugrahawati Anna Octavera Ardana Kurniaji Asrido, Farhan Ayi Santika Ayi Santika Ayu Dhita Juliadiningtyas, Ayu Dhita Bagus Rahmat Basuki Brite, Margie D. Meha D. Puspitaningtyas D. Wahjuningrum DEDI JUSADI Dendi Hidayatullah Dendi Hidayatullah Dendi Hidayatullah Dendi Hidayatullah, Dendi Dian Febriani Dian Hardiantho Dian Hardiantho Dinamella Wahjuningrum Dinar Tri Soelistyowati Dwi Hany Yanti Dwi Hany Yanti Dwi Yanti Eddy Supriyono Eni Kusrini Eni Kusrini Erni Susanti F.B.P. Sari F.H. Pasaribu Fachriyan Hasmi Pasaribu Fadhila Maharani Putri Fajrin, Anang Faoziyatunnisa, Nurul Fauzan Wahib Alsani Firdausi, Amalia Putri Firmansyah, Arif Lukman Fitriyah Husnul Khotimah Goro Yoshizaki Gusti Ngurah Permana Hanggara, Yudha Harits Atika Ariyatna Harton Arfah Haryanti Haryanti Haryanti Haryanti Hasan Nasrullah Hasan Nasrullah Hidayah, Winda Alfina Ibnu Bangkit Bioshina Suryadi Iis Widiani Ika Safitri Ikhsan Khasani Indah Mastuti Irzal Effendi ISNAWATI Isni Rahmatika Sari Julie Ekasari K. Sumantadinata Ketut Mahardika Ketut Sugama Ketut Sugama Khotimah, Fitriyah Husnul Komar Sumantadinata Kukuh Nirmala Kurnia Anggraini Rahmi L.S. Harti La Ode Muhammad Arsal Lili Sholihah Lilis Nurjanah M. Zairin Junior M.A. Suparman Mia Setiawati Min Rahminiwati Mira Mawardi Muchtar, Muthahharah MUHAMMAD AGUS SUPRAYUDI Muhammad Zairin Jr Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. Mulyani, Rahma MUNTI YUHANA N. Ashry N.A. Maswan Nadia Aulia, Nadia Nasrullah, Hasan Nurbariah Nurbariah Nurina Pratiwi Nurly Faridah Nurly Faridah NURWATI, BUNGA Odang Carman P. Giri P. Hariyadi Priyoutomo, Nur Bambang Putri, Elva Khairina Qorie Astria R.D. Soejoedono Rahma Mulyani Rahman Ramadhan, Afriani Ramadhani, Danika Athirah Ramadhina, Erina Tri Retno Damayanti Soejoedono Rizkiyanti, Ita S.H. Sholeh Sari Anggraeni, Sukma Sari Budi Moria Sembiring Sari Budi Moria Sembiring Sarjito - SATA YOSHIDA SRIE RAHAYU Sekar Ayu Chairunnisa Sekar Ayu Chairunnisa Sekar Sulistyaning Hadiwibowo Shavika Miranti Shella Marlinda Siti Khodijah Siti Murniasih Siti Zubaidah Slamet Budi Prayitno Soko Nuswantoro Sri Murtini Suhermanto, Achmad Sukenda Sukenda Sukenda, Sukenda, Sulasy Rohmy Taufiq Abdullah, Taufiq Tiara Puspa Anjani Tira Silvianti Titin Kurniasih Trian Rizky Febriansyah Tuti Sumiati Tuti Sumiati Utomo, Nurbambang Priyo Uttari Dewi Wahyuwardani , Sutiastuti Wasmen Manalu Wida Lesmanawati WIDANARNI WIDANARNI Y. Hadiroseyani Y. Kuswardani Yanti Inneke Nababan Yanti, Mauliza Yulianti, Susy Anisa Yuni Puji Hastuti Zakki Zainun Zulhelmi, Arif

Title

Found 77 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 77 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 77 Documents
Search