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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Jurnal Veteriner Jurnal Perikanan Kelautan Jurnal Natur Indonesia Journal of Aquaculture Management and Technology BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Ilmu-Ilmu Perikanan dan Budidaya Perairan SITEKIN: Jurnal Sains, Teknologi dan Industri EDULITE: Journal of English Education, Literature and Culture Omni-Akuatika JURNAL CITRA KEPERAWATAN Jurnal Perikanan Universitas Gadjah Mada Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur Creative Research Journal Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Journal of Aquaculture and Fish Health Jurnal Kridatama Sains dan Teknologi Jurnal Magister Pendidikan Matematika (JUMADIKA) Jurnal Abdi Insani Jurnal Ilmiah MEA (Manajemen, Ekonomi, dan Akuntansi) Jurnal Kedokteran Hewan Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat Jurnal Terapis Gigi dan Mulut Jurnal Karya Generasi Sehat Journal of Fisheries & Marine JPEMAS: Jurnal Pengabdian Kepada Masyarakat Jurnal Ilmu Kesehatan

Sri Nuryati

Bogor Agricultural University

Author-ID : 336092

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Efficacy of Streptococcus agalactiae vaccine strains N3M and N4M in fry tilapia infected by different strains of S. agalactiae Sukenda, Sukenda,; Firmansyah, Arif Lukman; Rahman, Rahman,; Nuryati, Sri; Hidayatullah, Dendi
Jurnal Akuakultur Indonesia Vol. 17 No. 2 (2018): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Streptococcus agalactiae is a major bacterial streptococcosis disease that infects tilapia. This study aimed to analyze a specific and nonspecific immune system in fry tilapia that has been given with S. agalactiae vaccine from N3M and N4M strain and examine the protective immunity against S. agalactiae N3M, N4M, N17O, NK1, and N14G strains infection. Fry tilapia used in this study has the weight of 7.086±0.948 g and length of 7.443±0.353 cm. The S. agalactiae strains that used were N3M, N4M, N17O, NK1,and N14G. Fry tilapia was vaccinated through intraperitoneal injection method with 0.1 mL per fish of N3M and N4M vaccines. Fish reared in aquarium sizing of 60×30×50 cm3 with a density of 10 fishes aquarium-1. Two weeks after vaccination, fry tilapia was tested with 0.1 mL fish-1 of each N3M, N4M, N17O, NK1, and N14G strains through intraperitoneal injection method. Antibody level measured with indirect enzym-linked immunosorbent assay (ELISA) method. The result of antibody level in vaccinated fish after vaccination of N3M, N4M vaccine, N3M and N4M control were 0.767; 0.743; 0.587; and 0.544, respectively. Relative percent survival in N3M vaccinated fish after challenged with N3M and N4M was 87.50% dan 64.70%, respectively, otherwise in N4M vaccinated fish was 62.50% dan 76.47%, respectively. N3M and N4M vaccine strain have better protection as only if it tested with similar bacteria strain. Keywords: formalin-killed cell, tilapia, protection, Streptococcus agalactiae, strains ABSTRAK Streptococcus agalactiae merupakan bakteri utama penyakit streptococcosis yang menginfeksi ikan nila. Tujuan penelitian ini adalah menganalisis sistem imun spesifik dan nonspesifik pada benih ikan nila yang diberi vaksin S. agalactiae strain N3M dan N4M serta mengkaji imunitas protektif terhadap infeksi S. agalactiae strain N3M, N4M, N17O, NK1, dan N14G. Benih ikan nila yang digunakan memiliki bobot 7,086±0,948 g dan panjang 7,443±0,353 cm. Bakteri yang digunakan adalah S. agalactiae strain N3M, N4M, N17O, NK1,danN14G. Benih ikan nila divaksinasi menggunakan metode injeksi pada bagian intraperitoneal sebanyak 0,1 mL ekor-1 vaksin N3M dan N4M. Pemeliharaan dilakukan pada akuarium berukuran 60×30×50 cm3 dengan kepadatan 10 ekor akuarium-1. Dua minggu setelah vaksinasi benih ikan diuji tantang dengan strain bakteri N3M, N4M, N17O, NK1, danN14G menggunakan metode injeksi pada bagian intraperitoneal dengan dosis 0,1 mL ekor-1. Level antibodi diukur dengan metode indirect enzym-linked immunosorbent assay (ELISA). Hasil menunjukkan level antibodi ikan setelah vaksinasi pada perlakuan vaksin N3M, N4M, kontrol N3M, dan kontrol N4M berturut-turut 0,767; 0,743; 0,587; dan 0,544. Kelangsungan hidup relatif ikan yang divaksin N3M setelah diuji tantang dengan strain N3M dan N4M berturut-turut 87,50% dan 64,70%, sedangkan pada ikan yang divaksin N4M berturut-turut 62,50% dan 76,47%. Vaksin strain N3M dan N4M memiliki proteksi lebih baik jika diuji tantang dengan strain bakteri yang sama. Kata kunci: formalin-killed cell, Streptococcus agalactiae, strain, proteksi, nila

The use of immunostimulant from phycocyanin of Spirulina platensis to control motile aeromonad septicaemia (MAS) disease in common carp Cyprinus carpio Muchtar, Muthahharah; Sukenda, Sukenda; Nuryati, Sri; Hidayatullah, Dendi
Jurnal Akuakultur Indonesia Vol. 18 No. 1 (2019): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRAK Motile aeromonad septicaemia (MAS) adalah penyakit yang sering menyerang ikan mas Cyprinus carpio yang disebabkan oleh bakteri Aeromonas hydrophila. Penelitian ini bertujuan untuk menguji kinerja imunostimulan fikosianin dari Spirulina platensis dalam mengatasi penyakit MAS pada ikan mas. Penelitian ini terdiri atas dua tahap, pertama, pakan ikan dengan penambahan fikosianin 150 mg/kg, 250 mg/kg, dan 350 mg/kg pakan serta kontrol tanpa penambahan fikosianin. Setelah 14 hari, ikan diuji tantang dengan A.hydrophila. Tahap kedua, dosis terbaik dari penelitian pertama digunakan untuk pakan ikan masing-masing selama satu minggu/bulan, dua minggu/bulan, tiga minggu/bulan, dan dua minggu/bulan dengan interval satu minggu. Setelah 28 hari, ikan diuji tantang dengan A. hydrophila. Hasil penelitian pertama menunjukkan bahwa kelangsungan hidup relatif (RPS) ikan yang diberi pakan fikosianin 150 mg/kg, 250 mg/kg, dan 350 mg/kg pakan adalah 87,50%; 81,25%; dan 75,00%. Total eritrosit, hemoglobin, total leukosit, aktivitas fagositik, dan respiratory burst menunjukkan hasil yang lebih tinggi daripada kontrol untuk semua perlakuan pemberian fikosianin. Penelitian kedua menunjukkan bahwa nilai RPS ikan diberi pakan selama satu minggu/bulan, dua minggu/bulan, tiga minggu/bulan, dan dua minggu/bulan dengan interval satu minggu yaitu 65,38%; 69,23%; 76,92%; dan 69,23%. Respons imun ikan yang diberi fikosianin lebih tinggi daripada kontrol serta mampu menekan jumlah bakteri A. hydrophila di hati, ginjal, dan usus. Kesimpulan dari penelitian ini bahwa pemberian fikosianin sebanyak 150 mg/kg pakan selama tiga minggu/bulan memiliki nilai RPS tertinggi. Kata kunci: fikosianin, Spirulina platensis, Aeromonas hydrophila, Cyprinus carpio ABSTRACT Motile aeromonad septicaemia (MAS) is a major disease in common carp Cyprinus carpio caused by Aeromonas hydrophila. This study aimed to evaluate the performance of phycocyanin imunostimulant extracted from Spirulina platensis to control MAS disease in common carp. This study was conducted into two phases. First phase was conducted by adding 150 mg/kg, 250 mg/kg, 350 mg/kg feed phycocyanin dose, and 0 mg/kg feed phycocyanin dose as control treatment. Fish was challenged with pathogenic A.hydrophila after 14 days rearing. Second phase was conducted by applying the best dose obtained from the first phase added in the feed for feeding the fish in one week/month, two weeks/month, three weeks /month, and two weeks/month with one week interval. Fish was challenged with pathogenic A.hydrophila after 28 days rearing. First phase study result showed that the relative percent survival (RPS) for fish fed 150 mg/kg, 250 mg/kg, and 350 mg/kg phycocyanin dose were 87.50%, 81.25%, and 75.00% respectively. Total erythrocytes, hemoglobin, total leucocytes, phagocytic activity, and respiratory burst showed higher results than control treatment on all treated fish. The second phase study showed that fish fed one week/month, two weeks/month, three weeks/month, and two weeks/month with one week interval had RPS value 65.38%, 69.23%, 76.92%, and 69.23% respectively. The immune responses of treated fish were higher than control treatment, as well as the number of pathogenic A. hydrophila in the liver, kidney, and intestine. Fish fed with phycoyanin dose 150 mg/kg feed and three weeks/month administration had the highest RPS value. Keywords: Phycocyanin, Spirulina platensis, Aeromonas hydrophila, Cyprinus carpio

Identification and expression analysis of c-type and g-type lysozymes genes after Aeromonas hydrophila infection in African catfish Nasrullah, Hasan; Nababan, Yanti Inneke; Yanti, Dwi Hany; Hardiantho, Dian; Nuryati, Sri; Junior, Muhammad Zairin; Ekasari, Julie; Alimuddin, Alimuddin
Jurnal Akuakultur Indonesia Vol. 18 No. 2 (2019): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTLysozymes play an important role in the first line of defense in fish and potentially used as an immunity status biomarker and immune responses evaluation in fish, which often found in two types, i.e. chicken-type and goose-type (c- and g-type, respectively). To recent, the information related to the sequences and the expression analysis of the c- and g-type lysozyme genes in African catfish is still limited. In the present study, we report a partial cloning and mRNA expression analysis of c-type and g-type lysozymes in African catfish Clarias gariepinus. We have successfully cloned and partially identify the c-type, and g-type lysozyme genes of C. gariepinus, which consist of 594 and 560 of coding sequences, respectively. Catalytic and other conserved residues were identified by multiple sequences alignment and they showed high similarity with other teleost fish species. mRNA levels of the genes were analyzed by using qPCR method and their expressions in the spleen, liver, and head kidney were rapidly modulated after Aeromonas hydrophila injection, with different patterns were observed in each organ. These results confirmed that c- and g-type lysozymes played an important role in non-specific immunity against A. hydrophila infection. This study provided valuable information that can be used to understand the African catfish immune systems for better disease and stress management in C. gariepinus culture.Keywords: lysozymes, gene identification, gene expression, bacterial infection, African catfish ABSTRAKLisozim berperan dalam sistem pertahanan dini pada ikan dan sangat potensial digunakan sebagai marka status imunitas dalam evaluasi respons imun. Lisozim umum ditemukan dalam dua tipe pada ikan: tipe-ayam (tipe-c) dan tipe-angsa (tipe-g). Informasi terkait sekuens gen dan ekspresi gen kedua tipe lisozim pada ikan lele dumbo sangat terbatas. Pada penelitian ini, kami melaporkan kloning gen secara parsial, dan analisis ekspresi gen dari kedua tipe lisozim pada ikan lele dumbo C. gariepinus. Sekuens parsial gen lisozim tipe-c dan tipe-g yang berhasil diidentifikasi adalah sepanjang 594 dan 560 pasang basa. Situs katalitik dan residu khas memiliki tingkat kesamaan yang tinggi dengan spesies ikan yang lain. Analisis mRNA dilakukan dengan metode quantitative PCR (qPCR). Ekspresi kedua gen di jaringan ginjal depan, limpa, dan hati dengan cepat terinduksi pasca infeksi bakteri A. hydrophila dengan pola yang berbeda. Hasil ini menunjukkan bahwa lisozim tipe-c dan tipe-g memiliki peran yang sangat penting dalam sistem imun ikan lele dumbo terhadap infeksi A. hydrophila. Penelitian ini menghasilkan informasi penting yang dapat digunakan untuk mempelajari sistem imun ikan lele dumbo dan sebagai acuan dalam penanganan dan manajemen penyakit pada budidaya ikan lele dumbo.Kata kunci: lisozim, identifikasi gen, ekspresi gen, infeksi bakteri, ikan lele dumbo

Efficacy of Aeromonas hydrophila formalin-killed cells and lipopolysaccharides vaccines in maternal immunity of tilapia broodstock and the offspring resistance Mulyani, Rahma; Sukenda, Sukenda; Nuryati, Sri
Jurnal Akuakultur Indonesia Vol. 18 No. 2 (2019): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The study aimed to analyze the efficacy of Aeromonas hydrophila formalin-killed cells (FKC) and lipopolysaccharides (LPS) vaccines in maternal immunity of tilapia broodstock and the resistance of the offsprings. This study consisted of two consecutive studies. Firstly, the broodstocks were vaccinated with PBS (non-vaccinated), FKC, LPS, and combinations of FKC and LPS. Secondly, the offsprings from both the vaccinated and non‒vaccinated broodstock were vaccinated with the same vaccine for broodstock by immersion method. After vaccination, the offsprings were challenged with pathogenic A. hydrophila. Antibody level and lysozyme were measured in the broodstock, egg, and offsprings. In the first experiment, the result showed that vaccinated broodstock and their offsprings had delivered a significant antibody level and lysozyme activity compared with the control. The highest relative percent survival (RPS) of offspring resulted from the combination of FKC and LPS vaccinated broodstock at 5, 10, and 15 days post-hatching at 85.00%, 75.23%, and 67.56%, respectively. The second experiment showed vaccinated offsprings produced from vaccinated broodstock had a higher RPS than the RPS of vaccinated offsprings which produced from non-vaccinated broodstock. In conclusion, vaccination that using a combination of FKC and LPS vaccine in broodstock potentially improved the maternal immunity and protect their offspring from A. hydrophila infection. Keywords: Aeromonas hydrophila, antibody, maternal immunity, tilapia, vaccination ABSTRAK Penelitian ini bertujuan menganalisis efikasi vaksin formalin-killed cells (FKC) dan lipopolisakarida (LPS) Aeromonas hydrophila pada imunitas maternal induk ikan nila dan ketahanan benih yang dihasilkan. Dalam penelitian ini, terdapat dua penelitian. Pertama, induk ikan nila divaksin dengan PBS (non-vaksinasi), vaksin FKC, LPS, dan kombinasi vaksin FKC dan LPS. Benih selanjutnya diuji ditantang dengan menggunakan patogen A. hydrophila pada 5, 10, dan 15 hari pasca menetas. Kedua, benih yang dihasilkan oleh induk yang telah divaksinasi dan induk yang tidak divaksinasi tersebut divaksin dengan vaksin yang sama seperti pada induk dengan metode imersi. Setelah vaksinasi, keturunan diuji tantang dengan patogen A. hydrophila. Tingkat antibodi dan lisozim diukur pada induk, telur, dan benih. Pada percobaan pertama, hasilnya menunjukkan bahwa induk yang divaksinasi dan benih menunjukkan tingkat antibodi dan aktivitas lisozim yang signifikan dibandingkan dengan kontrol. Persentase kelangsungan hidup relatif (RPS) tertinggi benih yang dihasilkan dari induk yang divaksinasi FKC dan LPS masing-masing pada 5,10, dan 15 hari pascatetas pada 85,00%; 75,23%; dan 67,56%. Percobaan kedua menunjukkan bahwa benih yang divaksinasi dari induk yang divaksin menunjukkan RPS yang lebih baik daripada benih yang divaksinasi dari induk yang tidak divaksin. Vaksinasi menggunakan kombinasi FKC dan LPS pada induk berpotensi meningkatkan kekebalan induk dan melindungi benih dari infeksi A. hydrophila. Kata kunci: Aeromonas hydrophila, antibodi, imunitas induk, nila, vaksinasi

Efficacy of bivalent vaccine against black body syndrome (BBS) of barramundi Lates calcalifer B. Nugrahawati, Anis; Nuryati, Sri; Sukenda, Sukenda; Rahman, Rahman; Brite, Margie; Aditya, Tiya Widi
Jurnal Akuakultur Indonesia Vol. 18 No. 2 (2019): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Black body syndrome causes mass mortality in barramundi (Lates calcarifer) in Asia mariculture. This study aimed to evaluate the efficacy of bivalent vaccine derived from local isolate for black body syndrome (BBS) prevention on the Barramundi. The bacteria used in the bivalent vaccine were Pseudomonas stutzeri and Vibrio harveyi in a ratio of 50:50. Barramundi, 5‒6 cm in length, was intraperitoneally injected with bivalent vaccine and phosphate buffer saline (PBS). After a 21-day vaccination, fish was injected with pathogenic bacteria P. stutzeri, V. harveyi, the combination of P. stutzeri and V. harveyi with a dose of bacteria 107 CFU/fish. We observed relative percent survival (RPS), mortality, blood profile, antibody level, lysozyme activity, and histopathology of vaccinated fish. The result showed that vaccinated fish had higher antibody levels and lysozyme activity than control treatment (P>0.05). Vaccinated fish had RPS of 80.00%, 64.29%, 57.69%, after challenged test with P. stutzeri, V. harveyi, and combination of P. stutzeri and V. harveyi, respectively. Hemoglobin and hematocrit were not significantly different (P>0.05). However, the erythrocytes, leucocytes, and phagocytic activity were higher compared to there were higher erythrocytes, leucocytes, and phagocytic activity compared to control (P<0.05). As well as antibody level and lysozyme activity of vaccinated fish higher than control (P<0.05). In conclusion, the bivalent vaccine of P. stutzeri and V. harveyi could protect barramundi seed from BBS infection. Keywords: Lates calcalifer, bivalent vaccine, local isolate, Pseudomonas stutzeri, Vibrio harveyi ABSTRAK Black body syndrome menyebabkan kematian masal pada ikan kakap putih budidaya air laut di wilayah Asia. Tujuan dari penelitian ini adalah untuk menguji efikasi vaksin bivalen untuk penanggulangan black body syndrome pada benih ikan kakap putih. Bakteri yang digunakan untuk membuat vaksin bivalen yaitu Pseudomonas stutzeri dan Vibrio harveyi dengan rasio 50:50. Ikan kakap putih berukuran 5‒6 cm diinjeksi vaksin bivalen dan phosphat buffer saline secara intraperitoneal. Setelah 21 hari pemeliharaan, benih diuji tantang dengan bakteri patogen P. stutzeri, V. harveyi, campuran P. stutzeri dan V. harveyi dengan kepadatan bakteri sebesar 107 CFU/ikan. Parameter yang diamati meliputi relative percent survival (RPS), gambaran darah, titer antibodi, aktifitas lisosim, dan gambaran histopatologi benih kakap putih. Hasil penelitian menunjukkan bahwa ikan yang divaksin memiliki nilai titer antibodi dan aktifitas lisosim lebih tinggi dibandingkan kontrol (P<0.05). Ikan yang divaksin memilki RPS 80.00%, 64.29%, 57.69%, setelah diuji tantang dengan P. stutzeri, V. harveyi, campuran P. stutzeri dan V. harveyi. Nilai hemoglobin dan hematokrit tidak berbeda nyata (P>0.05). Titer antibodi dan aktifitas lisosim ikan yang divaksin lebih tinggi dari kontrol (P<0.05). Vaksin bivalen P. stutzeri dan V. harveyi dapat melindungi benih ikan kakap putih dari infeksi BBS. Kata kunci : Lates calcalifer, vaksin bivalen, Pseudomonas stutzeri, Vibrio harveyi

Evaluation of health status and meat quality of dumbo catfish Clarias gariepinus maintained using the biofloc system Fajrin, Anang; Utomo, Nurbambang Priyo; Ekasari, Julie; Nuryati, Sri
Jurnal Akuakultur Indonesia Vol. 19 No. 2 (2020): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.19.2.190-198

This research aimed to evaluate the health status, growth performance, and meat quality of African catfish Clariasgariepinus maintained in biofloc and nonbiofloc systems. This study applied 3 treatments with 4 replications, i.e.K500 (control, a non-biofloc system at a fish stocking density of 500/m3), BF500 (biofloc system at a fish stockingdensity of 500/m3), and BF700 (biofloc system at a fish stocking density of 700 /m3). The initial body length andbodyweight of the fish used in this experiment were 10–12 cm and 10–12g, respectively. Feeding was done byusing a commercial feed containing 29.76% protein content. The results showed that fish survival in treatmentsBF500 and BF700 were significantly different (P<0.05) from the control. The growth rate of African catfish in thecontrol (K) was the lowest (3.64 ± 0.56%) among the treatments. The results of the organosensory test showed thatthe application of biofloc systems produced a higher quality of fish meat with a range value of 7–9 compared to thatof the control with a range of 6–7. In conclusion, the application of biofloc systems could improve the fish healthstatus, growth performance, and meat quality of African catfish. Keywords: biofloc systems, Clarias gariepinus, density, health status, meat quality ABSTRAK Penelitian ini bertujuan mengevaluasi status kesehatan, kinerja pertumbuhan, dan kualitas daging ikan lele AfrikaClarias gariepinus yang dipelihara menggunakan sistem bioflok dan nonbioflok. Penelitian ini menggunakan 3perlakuan dengan 4 kali ulangan, yang terdiri dari: K500 (kontrol, sistem nonbioflok dengan kepadatan ikan 500ekor/m3), perlakuan BF500 (sistem bioflok dengan kepadatan ikan 500 ekor/m3), dan perlakuan BF700 (sistembioflok dengan kepadatan ikan 700 ekor/m3). Ikan yang digunakan berukuran panjang dan bobot awal masingmasing10–12 cm dan 10–12 g. Pemberian pakan dilakukan dengan menggunakan pakan komersil dengan 29,76%protein. Hasil penelitian menunjukan tingkat kelangsungan hidup antara perlakuan BF500 dan BF700 berbeda nyata(P<0.05) dengan perlakuan K500. Laju pertumbuhan ikan lele pada perlakuan K500 lebih rendah dibandingkanperlakuan lain. Hasil pengujian organosensory menunjukkan bahwa ikan yang dipelihara dalam sistem bioflokmenghasilkan kualitas daging yang lebih tinggi dengan kisaran nilai 7–9 dibandingkan dengan ikan K500 dengannilai 6–7. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa aplikasi sistem bioflok dapat meningkatkanstatus kesehatan, laju pertumbuhan, dan kualitas daging ikan lele Afrika. Kata kunci: bioflok, Clarias gariepinus, kepadatan, kualitas daging, status kesehatan

The effectiveness of cinnamon powder and cinnamon leaf extract to prevent Aeromonas hydrophila infection on striped catfish Pangasianodon hypophthalamus Susanti, Erni; Wahjuningrum, Dinamella; Nuryati, Sri; Setiawati, Mia
Jurnal Akuakultur Indonesia Vol. 20 No. 2 (2021): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.20.2.163-173

Striped catfish Pangasianodon hypophthalamus is one of the intensive cultured commodities. Disease outbreak becomes inevitable to prevent in a fish culture. One of the most frequent disease occurred in striped catfish is the motile aeromonas septicemia (MAS) disease caused by Aeromonas hydrophila. This study aimed to evaluate the effectiveness of 1% dietary cinnamon powder and 0.5% dietary cinnamon leaf extract on the immune response of striped catfish challenged with A. hydrophila. Striped catfish used in this study sizing of 5.80 ± 0.21 g. This study contained two phases, namely in vitro and in vivo tests. In vitro test contained inhibition zone and antibacterial tests, which demonstrates that 1% cinnamon powder and 0.5% cinnamon leaf extract are effective to inhibit A. hydrophila activity. In vivo test contained four treatments, i.e fish fed with 1% cinnamon leaf powder supplemented diet; 0.5% cinnamon leaf extract supplemented diet, positive control diet, and negative control diet. Each treatment was performed in three replications. The result showed that 1% cinnamon leaf powder supplemented diet obtained the best results to enhance the immune response of striped catfish higher survival rate value at 83.33% than the positive control diet (P<0.05). Keywords: Aeromonas hydrophila, Cinnamomum burmannii, extract, Pangasianodon hypophthalmus, powder. ABSTRAK Ikan patin Pangasianodon hypophthalamus termasuk komoditas yang banyak dibudidayakan secara intensif. Kendala budidaya seperti penyakit pun sulit untuk dihindari. Salah satu jenis penyakit yang kerap menyerang ikan patin yaitu penyakit MAS (motile aermomonad septicaemia) yang disebabkan oleh Aeromonas hydrophila. Penelitian ini bertujuan untuk menguji efektivitas 1% (w/w) tepung dan 0,5% (w/w) ekstrak daun kayu manis dalam pakan sebagai upaya pencegahan infeksi bakteri A. hydrophila pada ikan patin. Ikan patin yang digunakan berukuran 5,80 ± 0,21 g. Penelitian ini terdiri dua tahap yaitu uji in vitro dan uji in vivo. Hasil uji in vitro terhadap aktivitas antibakteri menunjukkan bahwa dosis 1% (w/w) tepung daun kayu manis dan 0.5% (w/w) ekstrak daun kayu manis efektif dalam menghambat pertumbuhan A. hydrophila. Uji in vivo terdiri atas empat perlakuan yaitu pemberian pakan dengan penambahan tepung daun kayu manis 1% (w/w), pemberian pakan dengan penambahan ekstrak daun kayu manis 0,5% (w/w), kontrol positif, dan kontrol negatif dengan masing-masing perlakuan terdiri atas tiga ulangan. Hasil penelitian menunjukkan bahwa penambahan 1% (w/w) tepung daun kayu manis dalam pakan memberikan hasil terbaik dalam meningkatkan respons imun ikan patin dengan tingkat kelangsungan hidup sebesar 83,33% lebih tinggi dibandingkan kontrol positif (P<0,05). Kata kunci: Aeromonas hydrophila, Cinnamomum burmannii, ekstrak, Pangasianodon hypophthalmus, tepung

Kidney and liver histopathology of sea bass Lates calcarifer infected with black body syndrome-associated bacteria Nuryati, Sri; Yanti, Mauliza; Rahman; Wahyuwardani , Sutiastuti
Jurnal Akuakultur Indonesia Vol. 22 No. 2 (2023): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.22.2.170-178

Bacterial isolation was carried out on sea bass Lates calcarifer juvenile with black body syndrome (BBS) and isolates of Pseudomonas stutzeri L01, Vibrio harveyi, Bacillus cereus, Salinicoccus roseus, and Pseudomonas stutzeri L02 were obtained. Microscopic anatomical pathology of each bacterium needs to be done to determine its pathogenicity. This study aims to compare the degree of damage to sea bass juveniles' liver and kidney organs through histopathological observations due to bacterial infections that cause BBS. The degree of histopathological damage was determined by scoring histopathological changes in the tissue under a microscope with a magnification of 40×10 in five fields of view. The data obtained were analyzed using SPSS 24 software and further tested with Kruskal-Wallis and Post Hoc (Mann Whitney). The results showed that fish liver and kidney organs in all treatments experienced necrosis compared to the control treatment, but not significantly different between bacterial infection treatments. Histopathological observations of kidney and liver organs of sea bass juveniles infected with bacteria that cause BBS show necrosis damage that was classified as normal and mild. Keywords: bacteria, histopathology, Lates calcarifer ABSTRAK Isolasi bakteri telah dilakukan pada benih ikan kakap putih yang mengalami black body syndrome (BBS) dan diperoleh isolat bakteri Pseudomonas stutzeri L01, Vibrio harveyi, Bacillus cereus, Salinicoccus roseus, dan Pseudomonas stutzeri L02. Patologi anatomi mikroskopis dari masing-masing bakteri perlu dilakukan untuk mengevaluasi patogenitasnya. Penelitian ini bertujuan membandingkan tingkat kerusakan organ hati dan ginjal benih ikan kakap putih melalui pengamatan histopatologi akibat infeksi bakteri penyebab BBS. Derajat kerusakan histopatologi ditentukan dengan melakukan skoring perubahan histopatologi jaringan di bawah mikroskop dengan perbesaran 40×10 pada lima lapang pandang. Data dianalisis menggunakan software SPSS 24 dan diuji lanjut dengan Kruskal-Wallis dan Post Hoc (Mann Whitney). Hasil pengamatan menunjukkan bahwa organ hati dan ginjal ikan pada semua perlakuan infeksi mengalami nekrosis dibandingkan dengan perlakuan kontrol, tetapi tidak berbeda nyata antar perlakuan infeksi bakteri. Hasil pengamatan histopatologi organ ginjal dan hati benih ikan kakap putih yang diinfeksi dengan bakteri yang menyebabkan BBS menunjukkan kerusakan nekrosis yang tergolong pada derajat normal dan ringan. Kata kunci: bakteri, histopatologi, kakap putih

Application of probiotic microcapsules Bacillus cereus BR2 with different doses for the prevention of Aeromonas hydrophila NFC1 infection in catfish Clarias sp. Faoziyatunnisa, Nurul; Yuhana, Munti; Wahjuningrum, Dinamella; Nuryati, Sri; Afiff, Usamah
Jurnal Akuakultur Indonesia Vol. 23 No. 2 (2024): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.23.2.134-146

This study aims to analyze the administration of microencapsulated probiotic Bacillus cereus BR2 through feed atdifferent doses on the survival, immune response, and digestive enzyme activity of catfish Clarias sp. infected with Aeromonas hydrophila NFC1. The research design consisted of five treatments and three replications: (K-) feeding without the supplementation of probiotics fish injected with Phosphate-buffered saline solution; (K+) feeding without the supplementation of probiotics, fish injected with A. hydrophila NFC1 cell suspension (106 CFU/mL); (P1) feeding with supplementation of B. cereus BR2 microcapsules at a dose of 0.5% (w/w); (P2) feeding with the supplementation of B. cereus BR2 microcapsules at a dose of 1% (w/w; (P3) feeding with the supplementation of B. cereus BR2 microcapsules at a dose of 2% (w/w). All treatments of P1, P2, and P3 were intramuscularly injected with A. hydrophila NFC1 cell suspension. Catfish in average body weight of 3.73 ± 0.22 g were reared in tanks filled with 30 liters of water, at a density of 15 fish per container. A feed supplementation trial was performed for 40 days with feeding times three times a day; and a 5% feeding rate reevaluated based on biomass. Fish rearing was continued after the challenge test with A. hydrophila NFC1 for 10 days. The results showed that the application of supplemented feed containing B. cereus BR2 probiotic microcapsules increased the survival rate, immune response, and digestive enzyme activities of catfish which was infected with A. hydrophila NFC1, 2% microcapsule supplementation demonstrated the best result. Keywords: Aeromonas hydrophila NFC1, Bacillus cereus BR2, catfish, microcapsule, probiotics ABSTRAK Penelitian ini bertujuan untuk menganalisis pemberian mikrokapsul probiotik Bacillus cereus BR2 melalui pakan dengan dosis berbeda terhadap sintasan, respons imun, dan aktivitas enzim pencernaan ikan lele Clarias sp. yang diinfeksi Aeromonas hydrophila NFC1. Rancangan penelitian yang digunakan terdiri dari lima perlakuan dan tiga ulangan, yaitu (K-) pemberian pakan tanpa suplementasi probiotik dan diinjeksi PBS; (K+) pemberian pakan tanpa suplementasi probiotik dan diinfeksi A. hydrophila NFC1; (P1) pemberian pakan dengan suplementasi mikrokapsul B. cereus BR2 dosis 0,5% (b/b); (P2) pemberian pakan dengan suplementasi mikrokapsul B. cereus BR2 dosis 1%; (P3) pemberian pakan dengan suplementasi mikrokapsul B. cereus BR2 dosis 2%, masing-masing diinfeksi A. hydrophila NFC1. Benih lele dengan ukuran bobot rata-rata sekitar 3.73 ± 0.22 g dipelihara di dalam akuarium bervolume 30 L dengan kepadatan 15 ekor per wadah selama 40 hari, dengan pemberian pakan bersuplemen sebanyak tiga kali sehari, dengan 5% pemberian pakan yang dievaluasi berdasarkan bobot biomassa. Pemeliharaan ikan dilanjutkan setelah uji tantang dengan A. hydrophila NFC1 (106 CFU/mL) yang dilakukan hingga 10 hari pasca injeksi. Hasil penelitian menunjukkan aplikasi mikrokapsul probiotik B. cereus BR2 melalui pakan mampu meningkatkan sintasan, respons imun, dan aktivitas enzim pencernaan ikan lele yang diinfeksi A. hydrophila NFC1, dengan suplementasi mikrokapsul 2% sebagai hasil terbaik. Kata kunci: Aeromonas hydrophila NFC1, Bacillus cereus BR2, ikan lele, mikrokapsul, probiotik

Distribution of Vibrio parahaemolyticus in pacific white shrimp Litopenaeus vannamei through immersion as a natural infection model Sari Anggraeni, Sukma; Sukenda; Nuryati, Sri; Wahjuningrum, Dinamella
Jurnal Akuakultur Indonesia Vol. 23 No. 2 (2024): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.23.2.176-188

Vibrio parahaemolyticus is a pathogenic bacterium that indusces mortality is pacific white shrimp. This study aimed to analyze the distribution of V. parahaeomyticus in shrimp through immersion and evaluate, polymerase chain reaction, histopathology, and immune response. The study employed two treatments with four replicates each. Shrimp were infected through immersion with V. parahaemolyticus at a concentration of 107 CFU/mL for 30 minutes, followed by transferring the shrimp to a rearing container. Gill, hepatopancreas, gut, and hemolymph samples were collected at 6, 12, and 24 hours post-infection. Infected shrimp exhibited clinical symptoms such as a pale body and hepatopancreas, along with empty gut. Shrimp mortality reached 97.08%, while no mortality occurred in the control grouph. The abundance of V. parahaemolyticus in organs exceeded 105 CFU/mL. Histopathological examination revealed mild, moderate, and severe damage. PCR confirmation tests at 12 and 24 hours yielded positive results, with a DNA band at 230 bp. Immune responses, including total hemocytes, phagocytic activity, phenoloxidase, and respiratory burst, demonstrated significantly different results (P<0.05). It was concluded that the distribution of V. parahaemolyticus in the shrimp body, specifically in the gills, hepatopancreas, and gut, exceed 105 CFU/mL, as confirmed by a 230 bp DNA band in the PCR test. Tissue damage, manifested as lesions and necrosis, occurred in the gills, hepatopancreas, and gut. Additionally, the imun response of Vibrio-infected shrimp was found to be lower than that of uninfected shrimp (negative control). Keywords: distribution, immersion, Litopenaeus vannamei, Vibrio parahaemolyticus ABSTRAK Vibrio parahaemolyticus adalah bakteri patogen yang menyebabkan kematian pada udang vaname. Penelitian ini bertujuan menganalisis distribusi V. parahaemolyticus pada udang melalui perendaman serta kerusakan yang ditimbulkan melalui pengamatan gejala klinis, angka lempeng total bakteri, polymerase chain reaction, histopatologi dan respons imun. Penelitian menggunakan dua perlakuan dan empat ulangan. Udang diinfeksi melalui perendaman menggunakan V. parahaemolyticus 107 CFU/mL selama 30 menit, kemudian udang dipindah pada wadah pemeliharaan. Pengambilan organ insang, hepatopankreas, usus dan hemolim dilakukan pada jam ke-6, 12, dan 24 pascainfeksi. Udang terinfeksi memiliki gejala klinis seperti tubuh dan hepatopankreas pucat, usus kosong. Mortalitas udang mencapai 97.08% dan tidak ada kematian pada udang kontrol. Kelimpahan V. parahaemolyticus pada organ lebih dari 105 CFU/mL. Pengamatan histopatologi menunjukkan kerusakan ringan, sedang, hingga parah. Uji konfirmasi PCR jam ke-12 dan ke-24 menunjukkan hasil positif pada 230 bp. Respons imun seperti total hemosit, aktivitas fagositik, phenoloxidase, respiratory burst menujukkan hasil yang berbeda nyata (P<0.05). Disimpulkan bahwa distribusi V. parahaemolyticus di dalam tubuh udang pada terdapat pada insang, hepatopankreas dan usus lebih dari 105 CFU/mL dan terkonfirmasi uji PCR pada pita DNA 230 bp. Kerusakan jaringan seperti lesi dan nekrosis terjadi pada insang, hepatopankreas, dan usus. Respons imun udang yang terinfeksi Vibrio lebih rendah dibandingkan udang yang tidak terinfeksi (kontrol negatif). Kata kunci: distribusi, Litopenaeus vannamei, perendaman, Vibrio parahaemolyticus

Co-Authors , Alimuddin , Rahman, , , Rusli, , , Sofian, , , Yuliyanti . Kurdianto . Taukhid A. Santika A. Suwanto Ade Sunarma, Ade Adi Susanto, Riki Aditya, Tiya Widi Afif Abdurrahman Afiff , Usamah Agung Cahyo Setyawan Agus Oman Sudrajat Agussalim, Anita Mardiana Ahmad Beni Rouf Ahmad Firdaus, Ahmad Ahmad Muzaki Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin amperawati, metty Amperawati2, Metty Andi Tiara Eka Diana Puteri, Andi Tiara Eka Diana Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Anis Nugrahawati Anita Setyowati Srie Gunarti Ardana Kurniaji Asrido, Farhan Astu Rahman, Waljuni Ayi Santika Ayi Santika Ayu Dhita Juliadiningtyas, Ayu Dhita Bagus Rahmat Basuki Brite, Margie BUNGA NURWATI D. Meha D. Puspitaningtyas D. Wahjuningrum Dadi, Oswaldus Danan, Danan DEDI JUSADI Dendi Hidayatullah Dendi Hidayatullah Dendi Hidayatullah Dendi Hidayatullah, Dendi Dian Febriani Dian Hardiantho Dinamella Wahjuningrum Dinar Tri Soelistyowati Dwi Hany Yanti Dwi Hany Yanti Eddy Supriyono Eko Darma Elma yulius Elman Efzi, Agno Eni Kusrini Eni Kusrini Erni Susanti F.B.P. Sari F.H. Pasaribu Fachriyan Hasmi Pasaribu Fajar Prihesnanto, Fajar Fajrin, Anang Falah, Fazrul Faoziyatunnisa, Nurul Fateah, Nur Fauzan Wahib Alsani Firdausi, Amalia Putri Firmansyah, Arif Lukman Fitriyah Husnul Khotimah Goro Yoshizaki Gusti Ngurah Permana Habibah, Siti Sab'atul Habibah, Siti sabatul Hanggara, Yudha Harton Arfah Haryanti Haryanti Haryanti Haryanti Hasan Nasrullah Hidayah, Winda Alfina Ibnu Bangkit Bioshina Suryadi IDA RAHMAWATI Iis Widiani Ikhsan Khasani Indah Mastuti ISNAWATI Isnawati Isnawati Isni Rahmatika Sari Julie Ekasari K. Sumantadinata Kai, Meggy Wulandari Ketut Mahardika Ketut Sugama Ketut Sugama Khotimah, Fitriyah Husnul Kisworo Utami, Naning Komar Sumantadinata Kukuh Nirmala L.S. Harti La Ode Muhammad Arsal Lili Sholihah Lilis Nurjanah M. Zairin Junior M.A. Suparman Maharani, Rizqya Mia Setiawati Mira Mawardi Muchtar, Muthahharah MUHAMMAD AGUS SUPRAYUDI Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. Mukni Mukni, Mukni Mulyani, Rahma Munawarah, Husnadatul MUNTI YUHANA N. Ashry N.A. Maswan Nababan, Yanti Inneke Nadia Aulia, Nadia Nasrullah, Hasan Ninik Paryati Nisa, Hilwatun Nugroho, Sigit Dani Nur Rahmawati Nurbariah Nurbariah Nurhasanah Nurina Pratiwi Nurly Faridah Nurly Faridah NURWATI, BUNGA Odang Carman P. Giri P. Hariyadi Priyoutomo, Nur Bambang Puteri, Rafilah Putri, Elva Khairina Putri, Fadhila Maharani Qorie Astria R.D. Soejoedono Raharjo, Pamuji Gesang Rahma Mulyani Rahmadiniyah, Rahmadiniyah Rahman Rahman, Waljuni Astu Rahmi, Kurnia Anggraini Rahminiwati, Min Ramadhan, Afriani Ramadhani, Danika Athirah Ramadhina, Erina Tri Retno Damayanti Soejoedono Rizkiyanti, Ita S.H. Sholeh Said, Fahmi Sari Anggraeni, Sukma Sari Budi Moria Sembiring Sari Budi Moria Sembiring Sarjito - SATRIYAS ILYAS Sekar Ayu Chairunnisa Sekar Ayu Chairunnisa Sekar Sulistyaning Hadiwibowo Septia Armayani, Dilla Siti Khodijah Siti Murniasih Siti Salamah Siti Zubaidah Slamet Budi Prayitno Soko Nuswantoro sri murtini . Suhermanto, Achmad Sukenda . Sukenda . Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda, Sukenda, Suryani, Dessy Rizki Taufiq Abdullah, Taufiq Tiara Puspa Anjani Tira Silvianti Titin Kurniasih Trian Rizky Febriansyah Tuti Sumiati Tuti Sumiati Ulfah, Rasuna Utami, Naning K. Utomo, Nurbambang Priyo Uttari Dewi Wahyuwardani , Sutiastuti Wasmen Manalu Wida Lesmanawati WIDANARNI WIDANARNI Y. Hadiroseyani Y. Kuswardani Yanti, Mauliza Yuni Puji Hastuti Yusril Pratama, Muhammad Zakki Zainun Zulhelmi, Arif

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