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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vegetalika Jurnal Ilmu Pertanian Agrikultura Jurnal Ilmu Dasar Jurnal Natur Indonesia AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Agrovigor: Jurnal Agroekoteknologi Biogenesis: Jurnal Ilmiah Biologi CEMARA Journal of Degraded and Mining Lands Management AGRIVITA, Journal of Agricultural Science Jurnal Agro Planta Tropika UNEJ e-Proceeding Jurnal Penelitian Pertanian Terapan Agriprima : Journal of Applied Agricultural Sciences Jurnal Penelitian Pendidikan IPA (JPPIPA) Agrin : Jurnal Penelitian Pertanian SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Jurnal Agrotek Tropika AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Pengabdian Magister Pendidikan IPA Jurnal Kreativitas PKM jurnal Ilmu Pertanian (Agricultural Science) J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Jurnal Agrotek Indonesia Agroteknika Agro Bali: Agricultural Journal Jurnal Pengabdian Masyarakat IPTEKS Jurnal Bioindustri Jurnal Pengabdian Masyarakat Sasambo BIOVALENTIA: Biological Research Journal Journal of Applied Agricultural Science and Technology Prosiding Seminar Nasional Pengabdian Kepada Masyarakat PAPUMA: Journal of Comunnity Services Biosaintifika: Journal of Biology & Biology Education Journal of Bioindustry Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Natur Indonesia
Restanto, Didik Pudji
Prodi Agronomi, Fakultas Pertanian, Universitas Jember
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Journal : Jurnal Agro

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Respon kultur antera tembakau terhadap media bernutrisi dan AgNPs secara in vitro Khozin, Mohammad Nur; Ahnaf, Yusuf Dary; Dewanti, Parawita; Restanto, Didik Pudji; Iryono
Jurnal AGRO Vol. 12 No. 2 (2025)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/j.agro.49119

Abstract

Tanaman tembakau (Nicotiana tabacum L.) merupakan tanaman musiman bernilai ekonomi tinggi, namun produksinya di Indonesia terus mengalami penurunan dalam beberapa tahun terakhir. Penggunaan varietas hibrida dapat menjadi solusi karena memiliki produktivitas dan ketahanan penyakit yang lebih baik, tetapi pembentukannya secara konvensional memerlukan waktu lama. Teknik kultur antera dapat digunakan untuk mempercepat pembentukan galur murni karena menghasilkan tanaman homozigot hanya dalam satu generasi. Penelitian ini bertujuan untuk menganalisis interaksi antara jenis media kultur (MS dan Chu’s N6) dengan penambahan beberapa konsentrasi Nanopartikel silver (AgNPs) yaitu 0,0; 2,5; 5,0; 7,5; dan 10,0 mg L⁻¹ terhadap pertumbuhan antera tembakau secara in vitro. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 10 perlakuan dan 3 ulangan, kemudian data dianalisis menggunakan ANOVA dan diuji lanjut DMRT dengan taraf kepercayaan 95%. Hasil penelitian menunjukkan bahwa media MS merupakan media terbaik dalam mempercepat dan memperbanyak induksi swelling, kalus, tunas, dan akar, sedangkan konsentrasi AgNPs terbaik diperoleh pada 2,5 mg L⁻¹ yang mampu meningkatkan respons pertumbuhan serta sedikit mengurangi tingkat kontaminasi media. Hasil penelitian ini menunjukkan bahwa kombinasi media MS dan AgNPs dosis rendah mampu meningkatkan efisiensi regenerasi antera tembakau dan berpotensi digunakan sebagai protokol standar dalam produksi galur murni secara cepat, efisien, dan steril di laboratorium kultur jaringan. ABSTRACT Tobacco (Nicotiana tabacum L.) is a seasonal crop with high economic value, but its production in Indonesia has continued to decline in recent years. The use of hybrid varieties can be a solution because they have better productivity and disease resistance, but their conventional formation requires a long time. Anther culture techniques can be used to accelerate the formation of pure lines because they produce homozygous plants in just one generation. This study aims to determine the interaction between the types of culture media (MS and Chu's N6) with the addition of several concentrations of silver nanoparticles (AgNPs), namely 0, 2.5, 5, 7.5, and 10 mg L⁻¹ on the growth of tobacco anthers in vitro. The study used a Completely Randomized Design (CRD) with 10 treatments and 3 replications, then the data were analyzed using ANOVA and further tested with DMRT at the 95% level. The results showed that MS medium was the best medium in accelerating and increasing the induction of swelling, callus, shoots, and roots, while the best concentration of AgNPs was obtained at 2.5 mg L⁻¹ which was able to increase the growth response and slightly reduce the level of media contamination. The findings of this study indicate that the combination of MS medium and low-dose AgNPs can increase the efficiency of tobacco anther regeneration and has the potential to be used as a standard protocol in the production of pure lines quickly, efficiently, and sterilely in tissue culture laboratories.
Co-Authors . Usmadi Adinda, Intan Dwi Agnes Natalia Wijaya Ahnaf, Yusuf Dary Aji, Seto Purnomo Alfarisy, Fariz Kustiawan Anang Syamsunihar Antono, Moh Nuri Aprila Iga Mufidah Arsyika, Izna Arya Wiranegara Azizah, Kunti Anis Azizah, Maria Bambang Kuswandi Bambang Sugiharto Bintoro, Jatmiko Hardi Budha, Al Sura Tri Budi Kristanto Budi Kriswanto Budi Kriswanto Budi Kriswanto Budi Kriswanto Budi Kriswanto, Budi Calista, Zalza Candra Prayoga, Mohammad Darah Pertami, Rindha Rentina Dinata, Gallyndra Fatkhu Dodi Wijaya Dwi Erwin Kusbianto Dwika Nano Hariyanto Etik Wukir Tini Fanata, Wahyu Indra Duwi Farlisa, Veronenci Yuliarbi Fathoni, Rizal Dwi Fenny Irawati Fifteen Aprila Fajrin Firdauzi, Sandy Al Firdha Narulita Alfian Hadi Paramu Haliza, Nurhayadatul Handayani, Etty Hanif Fatur Rohman Hanifah, Fairuz Luthfi HARDIAN SUSILO ADDY Harsanti, Restiani Sih Indraloka, Aldy Bahaduri Indri Fariroh Iryono Kacung Hariyono Ketut Anom Wijaya Khozim, Mohammad Nur Khozin, Mohammad Nur Kriswanto, Budi Kurniasari, Leli Kushariyadi Kushariyadi Laily Ilman Widuri, Laily Ilman Laksono Trisnantoro Luluk Noviana Lutfi Pramukyana Mochammad Wildan Jadmiko Mohammad Nur Khozim Mona, Muhammad Dima Say Muhammad Dima Say Mona Muhammad Nur, Kholid Rosyidi Munandar, Denna Eriani Murtaqib Murtaqib Nadiya, Nisma Riyadh Nafiah, Nur Lailin Nafisah Iqmatullah Natasha Florenika Nur Lailin Nafiah Nurfika Asmaningrum Nurhasna, Rifqi Pamungkas, Wahyu Eka Parawita Dewanti Parawita Dewanti Poppy Hartatie Hardjo Popy Hartatie Hardjo Prayoga, Mohammad Candra Putri, Widya Kristyanti Rahmawati Rahmawati Raisah Bani Ratnasari, Tri Refa Firgiyanto Refa Firgiyanto Resti, Ricke Windi Rizqi Maulana Rohman, Fadil Rusdiana, Riza Yuli Saputro Gumelar, Bachtiar Setiyono Setiyono Sholeh Avivi Sigit Soepardjono Sigit Soeparjono Simamora, Endah Cahyani Slameto . Slameto Slameto Slameto Slameto Slameto Slameto Sri Hartatik Sukri, M Zayin Suliswanto, Eko Nur Sundahri, Sundahri Susanto, Arnis Budi Thohirin, Ahmad Nazmi Thongiratama, Michael Anthony Tri Agus Siswoyo TRI HANDOYO Tri Handoyo Tri Ratnasari Tri Rini Kusparwanti, Tri Rini Tri Widyastuti Ubaidillah, Mohammad Ummi Solikhah, Ummi Vega Kartika Sari Veronenci Yuliarbi Farlisa Wahyu Indra Duwi Fanata Widya Kristiyanti Putri Widya Kristiyanti Putri Wildan Muhlison, Wildan Wulanjari, Distiana Yuli Witono Zahro, Fauziatuz