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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vegetalika Jurnal Ilmu Pertanian Agrikultura Jurnal Ilmu Dasar Jurnal Natur Indonesia AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Agrovigor: Jurnal Agroekoteknologi Biogenesis: Jurnal Ilmiah Biologi CEMARA Journal of Degraded and Mining Lands Management AGRIVITA, Journal of Agricultural Science Planta Tropika UNEJ e-Proceeding Jurnal Penelitian Pertanian Terapan Agriprima : Journal of Applied Agricultural Sciences Jurnal Penelitian Pendidikan IPA (JPPIPA) Agrin : Jurnal Penelitian Pertanian SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Jurnal Agrotek Tropika AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Pengabdian Magister Pendidikan IPA Jurnal Kreativitas PKM jurnal Ilmu Pertanian (Agricultural Science) J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Jurnal Agrotek Indonesia Agroteknika Agro Bali: Agricultural Journal Jurnal Pengabdian Masyarakat IPTEKS Jurnal Bioindustri Jurnal Pengabdian Masyarakat Sasambo BIOVALENTIA: Biological Research Journal Journal of Applied Agricultural Science and Technology Prosiding Seminar Nasional Pengabdian Kepada Masyarakat PAPUMA: Journal of Comunnity Services Biosaintifika: Journal of Biology & Biology Education Journal of Bioindustry Jurnal Natur Indonesia
Restanto, Didik Pudji
Prodi Agronomi, Fakultas Pertanian, Universitas Jember
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Journal : UNEJ e-Proceeding

 1 
Cloning coat protein gene of CBSD (cassava brown streak disease) at cassava (Manihotesculentum) Restanto, Didik Pudji; ., Slameto; Kriswanto, Budi; Addy, Hardian Susilo; Handoyo, Tri
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Cassava Brown Streak Disease (CBSD) is a major disease in cassava plants which have the serious problems in cassava plantations in the world, especially in Africa, Tanzania and India (Wassawaet., al, 2010). In Indonesia, the virus is still not optimal yet in the handling. The disease is present in plants that can destructive cassava leaves, stems and tubers.  It was greatly reduces the quality and production in the world such as India.  The decrease of cassava yield can reach 100% due to disease of CBSD (Lopez, 2003). The primer was designed from the coat protein gene of CBSD with a distance of 380 bp (Abarshiet.,al, 2012). The primers designed the forward primer (GGARCCRATGTAYAAATTTGC) and Reverse (GCWGCTTTTA  TYACAAAMGC). The RNA isolation have been used Plant Virus RNA Kit (Geneaid).  The CBSD RNA concentration around 55,2ng/ul.  The RT PCR program were one cycle of RT PCR reaction (45oC for 30 min), denaturation (45oC for 5 min) and 30 cycles for denaturation (94oC for 1 min), annealing (52oC for 30 sec), extention  (72oC for 1 min).  The results showed a single band of about 380 bp which is the  distance between the two primers were tested.  The multiplication shoot around 5 shoots per meristem explants with a combination of 0.5 ppm and 0.1 ppm BAP GA3 Keyword : Cassava Brown Streak Disease (CBSD), CASSAVA (Manihotesculentum), RT PCR
MORPHOLOGICAL AND PHYSIOLOGICAL CHARACTERS OF CASSAVA (MANIHOT ESCULENTA CRANTZ) WHICH WET TOLERANT Rahmawati Rahmawati; Tri Agus Siswoyo; Didik Puji Restanto; Sri Hartatik; Sigit Soeparjono; Sholeh Avivi
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

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Weather conditions often unstable changed and increase the floods of agricultural land. Land which was originally drought will experience wetness and need variety of a plant that wetness tolerant. This study were aimed to get cassava that wet tolerant and to study the morphological, physiological characters of cassava grown on wetness land. The experiment was conducted based on factorial random plot design that consisting two factors with five replications. The first factor were four varieties namely: V1 = Daun Ganja; V2 = Sawi Ketan; V3 = Kasesat Beracun; V4 = Gajah. The second factor was wet treatment consisting of 2 the condition that were K0 = as control, media with 100% field capacity; K1 = as wet treatment by watering of media maintain on -10 cm from media surface. The difference between treatments tested with Duncan Multiple Range Test (DMRT) with the probability 5%. Wet treatment started when plant aged was 2 months after planting, during eight weeks. The observations on variables based on morphological and physiological characters. Result showed that every varieties give the different response on wet treatment. Best response on wet tolerant variety shown by variety code of V2 (Sawi Ketan) were indicated by plant height, total leaf number produced, stem diameter, fresh weight of root, dry weight of root, fresh weight of shoot, dry weight of shoot, photosynthesis index, stomata conductivity and root volume.
Cloning coat protein gene of CBSD (cassava brown streak disease) at cassava (Manihotesculentum) Didik Pudji Restanto; Slameto .; Budi Kriswanto; Hardian Susilo Addy; Tri Handoyo
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Cassava Brown Streak Disease (CBSD) is a major disease in cassava plants which have the serious problems in cassava plantations in the world, especially in Africa, Tanzania and India (Wassawaet., al, 2010). In Indonesia, the virus is still not optimal yet in the handling. The disease is present in plants that can destructive cassava leaves, stems and tubers.  It was greatly reduces the quality and production in the world such as India.  The decrease of cassava yield can reach 100% due to disease of CBSD (Lopez, 2003). The primer was designed from the coat protein gene of CBSD with a distance of 380 bp (Abarshiet.,al, 2012). The primers designed the forward primer (GGARCCRATGTAYAAATTTGC) and Reverse (GCWGCTTTTA  TYACAAAMGC). The RNA isolation have been used Plant Virus RNA Kit (Geneaid).  The CBSD RNA concentration around 55,2ng/ul.  The RT PCR program were one cycle of RT PCR reaction (45oC for 30 min), denaturation (45oC for 5 min) and 30 cycles for denaturation (94oC for 1 min), annealing (52oC for 30 sec), extention  (72oC for 1 min).  The results showed a single band of about 380 bp which is the  distance between the two primers were tested.  The multiplication shoot around 5 shoots per meristem explants with a combination of 0.5 ppm and 0.1 ppm BAP GA3 Keyword : Cassava Brown Streak Disease (CBSD), CASSAVA (Manihotesculentum), RT PCR
THE PROPAGATION OF ORCHID THROUGH FORMATION OF PROTOCORM LIKE BODIES (PLB) FOR SUPPORTING THE RESQUE OF Phalaenopsis ORCHID IN INDONESIA Budi Kriswanto; Sigit Soeparjono; Didik Pudji Restanto
UNEJ e-Proceeding Proceeding of International Conference on Food Sovereignty and Sustainable Agriculture (FoSSA) 2017
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Indonesia is a tropical country that has large orchid germ plasm because it has tropicalforests along the equatorial regions (islands of Sumatra, Kalimantan, Sulawesi and Papua) Recently, there has been changed become oil palm plantations and climate change, which hascaused the extinction of orchid. The Phalaenopsis orchid is an orchid that has high exotic andcommercial value. The purpose of this study was to maintain the Phalaenopsis throughpropagation of Protocorm Like Bodies (PLB). This study used the treatment of different kindsof basal medium Murashige & Skoog (MS), ½ Murashige & Skoog (1/2 MS) and Vacin& Went(VW) and 6-benzylaminopurine (BAP) and α-naphthylacetic acid (NAA) concentrations ongrowth and propagation of PLB. The results showed sequentially VW, ½ MS and MS mediaprovided the best plb growth and media ½ MS provides regeneration plb into a good planletin which shoots and roots formed. Treatment of plant growth regulator of BAP: NAA effect ondiameter of plb colony and individual size of plb with 2.1 cm and 0.2 cm, respectively.
Co-Authors . Usmadi Adinda, Intan Dwi Agnes Natalia Wijaya Aji, Seto Purnomo Alfarisy, Fariz Kustiawan Anang Syamsunihar Antono, Moh Nuri Aprila Iga Mufidah Arsyika, Izna Arya Wiranegara Azizah, Kunti Anis Azizah, Maria Bambang Kuswandi Bambang Sugiharto Bintoro, Jatmiko Hardi Budha, Al Sura Tri Budi Kristanto Budi Kriswanto Budi Kriswanto Budi Kriswanto Budi Kriswanto Budi Kriswanto, Budi Calista, Zalza Candra Prayoga, Mohammad Darah Pertami, Rindha Rentina Dinata, Gallyndra Fatkhu Dodi Wijaya Dwi Erwin Kusbianto Dwika Nano Hariyanto Etik Wukir Tini Fanata, Wahyu Indra Duwi Farlisa, Veronenci Yuliarbi Fenny Irawati Fifteen Aprila Fajrin Firdauzi, Sandy Al Firdha Narulita Alfian Hadi Paramu Haliza, Nurhayadatul Handayani, Etty Hanif Fatur Rohman Hanifah, Fairuz Luthfi HARDIAN SUSILO ADDY Harsanti, Restiani Sih Indraloka, Aldy Bahaduri Kacung Hariyono Ketut Anom Wijaya Khozim, Mohammad Nur Khozin, Mohammad Nur Kriswanto, Budi Kurniasari, Leli Kushariyadi Kushariyadi Laily Ilman Widuri, Laily Ilman Laksono Trisnantoro Luluk Noviana Lutfi Pramukyana Mochammad Wildan Jadmiko Mohammad Nur Khozim Mohammad Nur Khozin Mohammad Nur Khozin Mona, Muhammad Dima Say Muhammad Dima Say Mona Muhammad Nur, Kholid Rosyidi Munandar, Denna Eriani Murtaqib Murtaqib Nadiya, Nisma Riyadh Nafiah, Nur Lailin Nafisah Iqmatullah Natasha Florenika Nur Lailin Nafiah Nurfika Asmaningrum Nurhasna, Rifqi Pamungkas, Wahyu Eka Parawita Dewanti Parawita Dewanti Poppy Hartatie Hardjo Popy Hartatie Hardjo Prayoga, Mohammad Candra Putri, Widya Kristyanti Rahmawati Rahmawati Raisah Bani Ratnasari, Tri Refa Firgiyanto Refa Firgiyanto Resti, Ricke Windi Rizqi Maulana Rohman, Fadil Rusdiana, Riza Yuli Saputro Gumelar, Bachtiar Setiyono Setiyono Sholeh Avivi Sigit Soepardjono Sigit Soeparjono Simamora, Endah Cahyani Slameto . Slameto Slameto Slameto Slameto Slameto Slameto Sri Hartatik Sukri, M Zayin Suliswanto, Eko Nur Sundahri, Sundahri Susanto, Arnis Budi Thohirin, Ahmad Nazmi Thongiratama, Michael Anthony Tri Agus Siswoyo TRI HANDOYO Tri Handoyo Tri Ratnasari Tri Rini Kusparwanti, Tri Rini Tri Widyastuti Ubaidillah, Mohammad Ummi Solikhah, Ummi Vega Kartika Sari Veronenci Yuliarbi Farlisa Wahyu Indra Duwi Fanata Widya Kristiyanti Putri Widya Kristiyanti Putri Wildan Muhlison, Wildan Wulanjari, Distiana Yuli Witono Zahro, Fauziatuz