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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Agrotrop : Journal on Agriculture Science METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Itepa : Jurnal Ilmu dan Teknologi Pangan Jurnal Veteriner Jurnal Biologi Udayana Bumi Lestari Ecotrophic, Journal of Environmental Science Jurnal Bios Logos Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology BIOTROPIA - The Southeast Asian Journal of Tropical Biology SIMBIOSIS Agrointek Biotropika Jurnal Biosains Pascasarjana Jurnal Biologi Universitas Andalas Biota ANDHARUPA REINWARDTIA Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Floribunda Advances in Tropical Biodiversity and Environmental Sciences Jurnal Aplikasi dan Inovasi Iptek (JASINTEK) Indigenous Biologi BIO-CONS: Jurnal Biologi dan Konservasi Journal of Natural Sciences BIO-CONS: Jurnal Biologi dan Konservasi Reinwardtia
MADE PHARMAWATI
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Veterinary Other

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Journal : SIMBIOSIS

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PENGAMATAN MORFOLOGI DAN ANATOMI BIBIT KAMBOJA JEPANG (Adenium sp.) AKIBAT PERENDAMAN BIJI DENGAN KOLKISIN Putu Yunita Putri Aryani; Made Pharmawati
SIMBIOSIS Vol 3 No 2 (2015)
Publisher : Jurusan Biologi Universitas Udayana

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Abstract

This research aimed to determine the effect of colchicineby seed immersion ondessert rose (Adenium sp.) seedling. Observation were done on morphological and anatomical characters. This research was conducted using colchicine concentration of 0%, 0.05%, 0.1%, and 0.15%. Each treatment had 10 replications. The parameters observed included seedling emergence, seedling height, number of leaves, leaf length, leaf width, and stomatal density of cotiledone. The results showed differences in morphological characters, led to the emergence of seeds on the ground inhibited by immersion in colchicine. Anatomically giving of colchicines cause a reduction instomatal density of cotiledone. Keywords: anatomy, colchicine, dessert rose, morphology
EKSTRAKSI DNA DARI HERBARIUM ANGGREK UUL SHOVI NURKAMILA; MADE PHARMAWATI
SIMBIOSIS Vol 2 No 1 (2014)
Publisher : Jurusan Biologi Universitas Udayana

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Abstract

DNA extraction is the first step to study plant systematic and biodiversity analysis usingmolecular markers. This study aimed to conduct DNA extraction from herbarium materialsusing different extraction methods. A total of 0.05 grams of herbarium powders ofCalantheemarginata (Blume) Lindl. and Goodyera procera(Ker-Gawl) Hook. (terrestrialorchid) were used for samples by three different methods. The first method was from Doyleand Doyle with modification of incubation time for 1,5 hours at 65oC and increasing EDTAconcentration to 50 mM. Second method was Dellaporta et al. with modification of incubationtime for 1,5 hours (at 65oC) and increasing EDTA concentration to 100 mM. Third methodwas Rogers and Bendich with modification of incubation time for 1,5 hours (65oC) andadding ethanol twice. The results of electrophoresis revealed that method of Doyle and Doyleobtained DNA from C. emarginata herbarium, while method from Rogers and Bendich,unfortunately it was inconsistent. The method from Dellaporta et al.obtained DNA from G.procera herbarium, while method from Doyle and Doyle revealed inconsistent DNA forG.procera. PCR-RAPD revealed the quality of DNA isolated using Doyle and Doyle methodwas not optimal, showed by unclear patterns of DNA bands. PCR-RAPD using DNA isolatedwith method from Rogers and Bendich revealed clearer DNA bands but only for small sizefragment.Keywords : orchid, DNA extraction, herbarium, PCR
KERUSAKANKROMOSOM BAWANG MERAH (Allium cepaL.) AKIBAT PERENDAMAN DENGAN ETIDIUM BROMIDA Eka Fibayani Imaniar; Made Pharmawati
SIMBIOSIS Vol 2 No 2 (2014)
Publisher : Jurusan Biologi Universitas Udayana

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Abstract

The aim of this research was to identify thedamage of onion’s(AlliumcepaL.) chromosomes causedbyethidiumbromide submersion for 6and12hoursat 500ppm. The methodused to study chromosome damage of onionroot tip wassquash technique. The result showed several types of chromosomedamagesuch as the formation of, micronuclei, nuclear buds and chromosome bridges. At 6 hours submersion,the average percentageof chromosomal damage was 2.99 %, while in submersion for 12 hours, the average percentage of chromosomal damage was 6.81 %. Keywords:Ethidium bromide, chromosome damage, Allium cepa L.
PEMILIHAN PRIMER RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) PADA PCR (POLYMERASE CHAIN REACTION) TANAMAN KAMBOJA (Plumeria sp.) Vanesa Martida; Made Pharmawati
SIMBIOSIS Vol 4, No 1 (2016)
Publisher : Jurusan Biologi Universitas Udayana

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Abstract

There are many variation of Plumeria sp. that grown in Bali. The genetic identity of Plumeria sp. need to be analysedusing molecular study for plant breeding purpose. DNA extraction and primer selection are basic steps for molecular studyespecially in identification and analysis of genetic diversity. The aim of this research was to determine RAPD primerssuitable for molecular analysis of Plumeria sp. This research used CTAB method with modification for DNA extraction. Thesamples were young leaves of Plumeria sp. dried using silica gel. The primers used were produced by University of BritishColumbia and Operon Primer Technology. The results showed that DNA concentration of Plumeria sp from dried leaves wasbetween 33-267 ng/?l. Out of seven primers tested, three primers UBC-127, UBC-250, and OPH-06 produced clear andscorable amplification products for further analyses.Keywords: DNA, Plumeria sp., RAPD primer
JENIS-JENIS LAMUN DI PANTAI LEMBONGAN, NUSA LEMBONGAN DAN ANALISISNYA DENGAN PCR RUAS rbcL Maliza Kurnia; Made Pharmawati; Deny S. Yusup
SIMBIOSIS Vol 3 No 2 (2015)
Publisher : Jurusan Biologi Universitas Udayana

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Abstract

Seagrasses in Bali are distributed on coastal areas of south east Bali coastal waters of Nusa Dua, Serangan Island,Sanur Beach and beaches in Nusa Lembongan. In Bali, it has been reported that there are eight species of seagrasses. Thisresearch aimed to identify seagrass species on Lembongan Beach based on morphological characters and optimize PCRcondition for molecular analysis. This research is a preliminary research on molecular method for seagrass analysis. Seagrasssampling was conducted in Lembongan Beach (in front of Ketut’s Losmen) in Nusa Lembongan, Nusa Penida Subdistrict,Bali Province. DNA extraction method used was the method of Doyle and Doyle with modifications. Result showed thatthere are five species found in Lembongan Beach, Nusa Lembongan. These seagrass are Cymodocea rotundata, Enhalusacoroides, Halodule pinifolia, Thalassodendron ciliatum and Thalassia hemprichii. DNA extraction resulted in high size ofDNA and smear DNA. Optimation of PCR reaction of rbcL fragment was done at DNA concentration of 30 ng and 50 ng.The electrophoresisof PCR products showed that DNA concentration of 50 ng had thicker band than concentration of 30 ng.Keywords: DNA extraction, Morphology, PCR rbcL, Seagrass
EKSTRAKSI DNA DARI SIKAT GIGI BERDASARKAN LAMA PEMAKAIAN DAN LAMA PENYIMPANAN SETELAH DIPAKAI Agriani Dewinta; I Ketut Junitha; Made Pharmawati
SIMBIOSIS Vol 10 No 1 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.06 KB) | DOI: 10.24843/JSIMBIOSIS.2022.v10.i01.p02

Abstract

In recent years in Indonesia there have been frequent disasters, including natural disasters, non-natural disasters, and social disasters that have resulted in many casualties. The identification process of victims who have experienced natural disasters, non-natural disasters, and social disasters often uses DNA. The primary identification method commonly used in DVI is DNA. Disaster Victim Identification (DVI) is a procedure for identifying victims who died from a mass disaster that can be scientifically accounted for and refers to the Interpol standard. Sources of DNA data consist of primary and secondary data sources. Toothbrush is a secondary source of DNA data. Toothbrush is commonly used as a source of comparative DNA samples selected in cases of identification of dead victims without a family as a comparison. In this study, it was shown that the time of use and the part of the toothbrush had no significant effect (P> 0.01) on the amount of DNA. The results of the quality test using gel electrophoresis were only 7 samples, namely 2b, 3a, 10a, 10b, 11a, 11b, 12b which showed that there was a thin band of luminescence and DNA smears. PCR-RAPD test was performed to test the extracted DNA could be amplified. PCR-RAPD results from 12 DNA samples, 7 samples were successfully amplified, indicating that the DNA extracted from a toothbrush could be amplified.
KEANEKARAGAMAN TANAMAN UMBI – UMBIAN YANG BERPOTENSI SEBAGAI PANGAN ALTERNATIF DI KECAMATAN RENDANG DAN BEBANDEM, KABUPATEN KARANGASEM, BALI Indah Tria Hoky; Ida Ayu Astarini; Made Pharmawati
SIMBIOSIS Vol 10 No 2 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (401.068 KB) | DOI: 10.24843/JSIMBIOSIS.2022.v10.i02.p01

Abstract

ABSTRAK Penelitian bertujuan untuk mengeksplorasi dan mengidentifikasi jenis – jenis umbi, cara pengolahan, pemanfaatan, karakteristik morfologi dan kandungan gizi dari tanaman umbi umbian yang terdapat di Kecamatan Rendang dan Kecamatan Bebandem, Kabupaten Karangasem, Bali. Metode yang digunakan yaitu metode deskriptif dengan teknik wawancara, observasi lapangan, eksplorasi, koleksi dan dokumentasi. Dari hasil penelitan ditemukan 9 jenis umbi-umbian yaitu Manihot esculenta Crantz., Ipomoea batatas L., Colocasia esculenta L., Xanthosoma sagittifolium (L.) Schott, Amorphophallus campanulatus BI., Dioscorea alata L., D. bulbifera L., D. hipsida Dennts, dan D. aculeata. Spesies Manihot esculenta terdapat tiga varian, Ipomoea batatas terdapat 5 varian, Xantosoma sagittifolium terdapat 4 varian, Colocasia esculenta 1 varian, Amorphophallus campalunatus 1 varian, Dioscorea alata 4 varian, Dioscorea aculeata 2 varian, Dioscorea hipsida 1 varian dan Dioscorea bulbifera 2 varian. Pemanfaatan setiap jenis umbi-umbian cukup bervariasi : ubi kayu, talas,dan ubi jalar, sebelum dikonsumsi diolah dengan cara direbus, dibakar, dikukus, digoreng, sedangkan 2 jenis lainnya yaitu gadung dan suweg memerlukan perlakuan khusus sebelum dikonsumsi karena jenis umbi-umbian tersebut dapat menyebabkan keracunan. Pemanfaatan organ-organ lain dari jenis umbi-umbian dapat digunakan sebagai sayuran (daun ubi kayu, daun ubi jalar, daun talas, tangkai daun talas), obat tradisional (uwi dan ubi jalar), pakan ternak (batang talas, daun ubi jalar) dan sebagai penunjang ekonomi keluarga. Deskripsi dan karakter morfologi setiap jenis umbi berbeda. Perbedaan karakter morfologi dilihat pada perawakan, umbi (bentuk, struktur, ukuran, warna), batang (bentuk, struktur, ukuran, warna), daun (bentuk, struktur, ukuran, warna). Semua tanaman umbi – umbian yang ditemukan bisa menjadi bahan pangan utama pengganti beras karena mempunyai kandungan gizi yang kompleks. Kata kunci: Manihot esculenta Crantz., Ipomoea batatas L., Colocasia esculenta L., Xanthosoma sagittifolium (L.) Schott, Amorphophallus campanulatus BI., Dioscorea spp.
ANALISIS KERAGAMAN GENETIK VARIETAS JAGUNG (Zea mays L.) HIBRIDA MENGGUNAKAN MARKA RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) Viryanando Evan Rahardja; Made Pharmawati; Ni Made Gari
SIMBIOSIS Vol 10 No 2 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.524 KB) | DOI: 10.24843/JSIMBIOSIS.2022.v10.i02.p09

Abstract

Analisis keragaman genetik sangat penting dalam perakitan tanaman jagung (Zea mays L) hibrida. Tujuan dari penelitian ini adalah untuk menganalisis keragaman genetik dari enam varietas jagung menggunakan teknik PCR-RAPD (Polymerase Chain Reaction- Random Amplified Polymorphic DNA). Varietas jagung hibrida yang digunakan dalam penelitian ini adalah varietas jagung Srikandi, Arumba, Mira, Magenta, dan Simba. Varietas jagung lokal yang berasal dari Pulau Rote, Provinsi Nusa Tenggara Timur digunakan sebagai pembanding. Benih ditanam untuk pengamatan karakteristik morfologi dan sampel ekstraksi DNA. DNA diekstraksi dengan metode CTAB dan PCR-RAPD dilakukan menggunakan 3 primer. Ukuran produk PCR ditentukan menggunakan kertas semilog. Keragaman genetik antar varietas dianalisis menggunakan program MVSP (Multi-Variate Statistical Package) dengan metode UPGMA (Unweighted Pair Group Method with Arithmetic Mean). Hasil PCR-RAPD memberikan jumlah pita DNA sebanyak 28 dengan ukuran 310 bp hingga 1550 bp bp dan menghasilkan 23 pita polimorfik. Berdasarkan dendrogram yang dihasilkan dari PCR-RAPD, terdapat satu klaster yang terdiri atas varietas Rote, Srikandi, Arumba, Mira, dan Magenta, serta satu klaster lain yang terdiri atas varietas Simba. Varietas lokal Rote dan varietas hibrida Srikandi memiliki jarak genetik terdekat, sementara varietas Simba memiliki jarak genetik terjauh.
IDENTIFICATION OF ‘TONGKOL’ FISH IN KEDONGANAN FISH MARKET, KUTA, BADUNG DISTRICT, BALI Aziz, Alifya Ibnu; Pharmawati, Made; Watiniasih, Ni Luh
SIMBIOSIS Vol 11 No 2 (2023)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2023.v11.i02.p06

Abstract

‘Tongkol’ fishes are species in the family Scombridae. There are many types of ‘tongkol’ fishes, namely longtail tuna, eastern little tuna, frigate tuna and bullet tuna. Morphologically, ‘tongkol’ species are similar to one another. This can result in errors in recording the production of ‘tongkol’ species. The purpose of this study was to identify ‘tongkol’ species collected from Kedonganan Market, Badung, Bali using the COI (Cytochrome C Oxidase Subunit I) gene. Two species of ‘tongkol’ fish were purchased from Kedonganan Market, Badung, Bali. DNA isolation was carried out from fish fins using chelex, followed by amplification of the COI gene segment. The PCR products were then electrophoresed and sequenced. DNA sequences were matched to the database using the Basic Local Alignment Search Tool (BLAST) found in NCBI. Extraction yielded DNA with a concentration of 5.91 ng/ml with a ratio of A260/A280 = 1.3 for sample 1, while for sample 2 the concentration of DNA was 6.27 ng/ml with a ratio of A260/A280 = 1.33. The resulting PCR product is about 700bp in size. The results of the COI gene sequences had a gene size length of 682 bp for both fish species. BLAST analysis yielded a percent identity of 99.84-100% with eastern little tuna (Euthynnus affinis) and bullet tuna (Auxis rochei).
Co-Authors A. A. Putri Ardyanti A.A.S. A. Sukmaningsih Agriani Dewinta Anak Agung Ayu Putri Ardyanti Anak Agung Gde Raka Dalem Andi Bahtiar Batti Andrianus Sembiring, Andrianus Aziz, Alifya Ibnu Bayu Adjie Bayu Adjie Bayu Adjie Bram Setyadji Deny Suhermawan Yusup Dian Catur Prayantini Dwi Nur Rikhma Sari Eka Fibayani Imaniar Eka Fibayani Imaniar Ema Hendriyani Fransiska Nitti Fransiskus Jimmy Roga Gde Evayanti, Luh GUIJUN YAN Gusti Ayu Putri Arnila I Gde Suryadi I Gede Putu Ardhana I Gusti Agung Eka Saraswati I Gusti Ayu Agung Pradnya Paramitha I Gusti Ngurah Kade Mahardika I Ketut Catur Wiguna I Ketut Junitha I Ketut Suada I Komang Adi Widyastama I MADE AGUS WIARTANA I Made Anom Sutrisna Wijaya I Made Murna I Putu Agus Tirta Cahyana I Putu Satwika I Wayan Arnata I Wayan Rosiana I. Setyawati I.G.A.S. Wahyuni I.M.A.S. Wijaya IAN JAMES MACFARLANE IDA AYU ASTARINI Ida Ayu Ratih Purnama Dewi Imaniar, Eka Fibayani Indah Tria Hoky Inna Narayani IRIANI SETYAWATI Joko Raharjo, Sentot Junita Hardini Ketut Queena Fredlina Luh Putu Wrasiati Made Ria Defiani Maharani, Rai Janaki Maliza Kurnia Maya Agustina Maya Agustina Mery Wintari Ngurah Intan Wiratmini Ni Kadek Dewi Rustini NI KADEK YUNITA SARI Ni LUH ARPIWI Ni Luh Ayu Jami Wistiani, Ni Luh Ayu Jami Ni Luh Putu Agustini Ni Luh Watiniasih Ni Luh Wayan Yulia Mirayanti NI MADE DIAN PRATIWI Ni Made Gari Ni Made Sastriyani Wiendra Ni Made Sastriyani Wiendra Ni Made Suartin Ni Nyoman Wirasiti Ni Putu Adriani Astiti Ni Putu Eka Krisnayanti Ni Wayan Ayu Wiartini Ni Wayan Karolina Ni Wayan Sudatri PATRICK FINNEGAN Putera, I Kadek Aridena Putu Angga Wiradana Putu Apriliani Putu Yunita Putri Aryani Retno Kawuri Rifky Firmansyah Anwar Satwika, I Putu Septarini Dian Anitasari Septarini Dian Anitasari Siddique, Kadambot.H.M. Tassanai Jaruwatanaphan Tassanai Jaruwatanaphan Tassanai Jaruwatanaphan, Tassanai Tiwi Wati Uslan UUL SHOVI NURKAMILA Vanesa Martida Vanesa Martida Viryanando Evan Rahardja WAHYU WIDOWATI Wawan Sujarwo WENNI SETYO LESTARI Wenni Setyo Lestari Wenni Setyo Lestari, Wenni Setyo Wijaya, Putu Austin Widyasari Wildan Mujahidul Basyar Yasuyuki Watano Yasuyuki Watano Yasuyuki Watano, Yasuyuki Yenni Ciawi Zaetuna, Hanifa Laila