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Journal : Jurnal Biologi Universitas Andalas

RAPD Primers Selection for Genetic Variation Analysis of Banana Plant (Musa spp.) I Ketut Catur Wiguna; Made Pharmawati
Jurnal Biologi Universitas Andalas Vol 9, No 2 (2021)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.9.2.47-53.2021

Abstract

Identification of genetic diversity using molecular markers is important for basic information for plant conservation. Banana is a fruit bearing plants that important for food sources in human life. This study aimed to determine the optimum conditions of  PCR-RAPD reaction and RAPD primers that suitable to amplify DNA fragmens. DNA isolation was done using modified of CTAB and chloroform isoamil alcohol. The samples used was young leaves of nine banana cultivar plants. Optimation was done using variety of DNA and MgCl2 concentration. Eight primers produced by Operon Primer Technology were tested. The DNA genomic concentration obtained was in the range of 23,3 ng/µl – 70 ng/µl. The optimum conditions of PCR-RAPD of banana plants that produce clear band were 50 ng/µl DNA template, 3 mM MgCl2 with the number of thermal cycles was 40 x. There were six RAPD primers that successfully amplified DNA : OPA 02, OPA 04, OPB 12, OPD 20, OPH 01, and OPH 03. The primer OPA-04 had the lowest resolving power value (4,4) , while  OPH 01 had the highest (11,3) resolving power.
RAPD Primers Selection for Genetic Variation Analysis of Banana Plant (Musa spp.) Wiguna, I Ketut Catur; Pharmawati, Made
Jurnal Biologi Universitas Andalas Vol 9 No 2 (2021)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.9.2.47-53.2021

Abstract

Identification of genetic diversity using molecular markers is important for basic information for plant conservation. Banana is a fruit bearing plants that important for food sources in human life. This study aimed to determine the optimum conditions of  PCR-RAPD reaction and RAPD primers that suitable to amplify DNA fragmens. DNA isolation was done using modified of CTAB and chloroform isoamil alcohol. The samples used was young leaves of nine banana cultivar plants. Optimation was done using variety of DNA and MgCl2 concentration. Eight primers produced by Operon Primer Technology were tested. The DNA genomic concentration obtained was in the range of 23,3 ng/µl – 70 ng/µl. The optimum conditions of PCR-RAPD of banana plants that produce clear band were 50 ng/µl DNA template, 3 mM MgCl2 with the number of thermal cycles was 40 x. There were six RAPD primers that successfully amplified DNA : OPA 02, OPA 04, OPB 12, OPD 20, OPH 01, and OPH 03. The primer OPA-04 had the lowest resolving power value (4,4) , while  OPH 01 had the highest (11,3) resolving power.
Co-Authors A. A. Putri Ardyanti A.A.S. A. Sukmaningsih Agriani Dewinta Anak Agung Ayu Putri Ardyanti Anak Agung Gde Raka Dalem Andi Bahtiar Batti Andrianus Sembiring, Andrianus Aziz, Alifya Ibnu Bayu Adjie Bayu Adjie Bayu Adjie Bram Setyadji Deny Suhermawan Yusup Dian Catur Prayantini Dwi Nur Rikhma Sari Eka Fibayani Imaniar Eka Fibayani Imaniar Ema Hendriyani Fransiska Nitti Fransiskus Jimmy Roga Gde Evayanti, Luh GUIJUN YAN Gusti Ayu Putri Arnila I Gde Suryadi I Gede Putu Ardhana I Gusti Agung Eka Saraswati I Gusti Ayu Agung Pradnya Paramitha I Gusti Ngurah Kade Mahardika I Ketut Catur Wiguna I Ketut Junitha I Ketut Suada I Komang Adi Widyastama I MADE AGUS WIARTANA I Made Anom Sutrisna Wijaya I Made Murna I Putu Agus Tirta Cahyana I Putu Satwika I Wayan Arnata I Wayan Rosiana I. Setyawati I.G.A.S. Wahyuni I.M.A.S. Wijaya IAN JAMES MACFARLANE IDA AYU ASTARINI Ida Ayu Ratih Purnama Dewi Imaniar, Eka Fibayani Indah Tria Hoky Inna Narayani IRIANI SETYAWATI Joko Raharjo, Sentot Junita Hardini Ketut Queena Fredlina Luh Putu Wrasiati Made Ria Defiani Maharani, Rai Janaki Maliza Kurnia Maya Agustina Maya Agustina Mery Wintari Ngurah Intan Wiratmini Ni Kadek Dewi Rustini NI KADEK YUNITA SARI Ni LUH ARPIWI Ni Luh Ayu Jami Wistiani, Ni Luh Ayu Jami Ni Luh Putu Agustini Ni Luh Watiniasih Ni Luh Wayan Yulia Mirayanti NI MADE DIAN PRATIWI Ni Made Gari Ni Made Sastriyani Wiendra Ni Made Sastriyani Wiendra Ni Made Suartin Ni Nyoman Wirasiti Ni Putu Adriani Astiti Ni Putu Eka Krisnayanti Ni Wayan Ayu Wiartini Ni Wayan Karolina Ni Wayan Sudatri PATRICK FINNEGAN Putera, I Kadek Aridena Putu Angga Wiradana Putu Apriliani Putu Yunita Putri Aryani Retno Kawuri Rifky Firmansyah Anwar Satwika, I Putu Septarini Dian Anitasari Septarini Dian Anitasari Siddique, Kadambot.H.M. Tassanai Jaruwatanaphan Tassanai Jaruwatanaphan Tassanai Jaruwatanaphan, Tassanai Tiwi Wati Uslan UUL SHOVI NURKAMILA Vanesa Martida Vanesa Martida Viryanando Evan Rahardja WAHYU WIDOWATI Wawan Sujarwo Wenni Setyo Lestari WENNI SETYO LESTARI Wenni Setyo Lestari, Wenni Setyo Wiguna, I Ketut Catur Wijaya, Putu Austin Widyasari Wildan Mujahidul Basyar Yasuyuki Watano Yasuyuki Watano Yasuyuki Watano, Yasuyuki Yenni Ciawi Zaetuna, Hanifa Laila