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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Agrotrop : Journal on Agriculture Science METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Itepa : Jurnal Ilmu dan Teknologi Pangan Jurnal Veteriner Jurnal Biologi Udayana Bumi Lestari Ecotrophic, Journal of Environmental Science Jurnal Bios Logos Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology BIOTROPIA - The Southeast Asian Journal of Tropical Biology SIMBIOSIS Agrointek Biotropika Jurnal Biosains Pascasarjana Jurnal Biologi Universitas Andalas Biota ANDHARUPA REINWARDTIA Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Floribunda Advances in Tropical Biodiversity and Environmental Sciences Jurnal Aplikasi dan Inovasi Iptek (JASINTEK) Indigenous Biologi BIO-CONS: Jurnal Biologi dan Konservasi Journal of Natural Sciences BIO-CONS: Jurnal Biologi dan Konservasi Reinwardtia
MADE PHARMAWATI
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Veterinary Other

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Journal : METAMORFOSA Journal of Biological Sciences

 1 
Tumbuhan Pewarna Alami Dan Pengolahannya Pada Tenun Ikat Amarasi Di Desa Tunbaun, Kecamatan Amarasi Barat, Kabupaten Kupang, Nusa Tenggara Timur Fransiska Nitti; Junita Hardini; Made Pharmawati
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p17

Abstract

This study aims to identify the types of plants, its parts that are used and the processing methods for amarasi weaving fabrics. Data collection in this study were carried out in of the village of Tunbaun, West Amarasi Sub-district, Kupang District, East Nusa Tenggara as one of the centers for the production of amarasi woven fabrics. Qualitative method was used in this study with interview, observation, documentation and literature study techniques. The results showed that the weaving craftsmen in Tunbaun village, used four types of plants as natural dye for weaving including noni root bark (Indigofera tinctoria L., Fabaceae), indigo leaves (Indigofera tinctoria L., Fabaceae), young teak leaves (Tectona grandis L.f., Lamiaceae) and turmeric rhizome (Curcuma domestica Val., Zingiberaceae). Processing of ground noni root bark, then boiled in water to get a red color. Indigo leaves are soaked for 2x24 hours, added lime and let with solution and allowed to stand for 24 to obtain a blue indigo paste. Young teak leaves are boiled in water until they get a purple color. While the turmeric rhizome is grated and then boiled in water to get a yellow color. Keywords: Amarasi, Natural Dyes, Weaving
Keragaman Genetik dan Hubungan Kekerabatan Ayam Lokal Berdasarkan Penanda RAPD Mery Wintari; Made Pharmawati; Ngurah Intan Wiratmini
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p13

Abstract

Indonesian local chicken is one kind of germplasm that has been utilized by the community for various needs, such as consumption, ceremonial, collection, and cockfighting. To maintain its sustainability, an inventory of genetic diversity can be made using molecular markers. This study aimed to determine the genetic diversity and relationship between four Indonesian local chickens: ayam kampung, ayam ketawa, ayam kate lokal, and ayam bekisar kangean, based on RAPD marker. DNA was extracted from blood samples of 12 local chickens consisting of 3 ayam kampung, 3 ayam ketawa, 3 ayam kate lokal, and 3 ayam bekisar kangean using Genetic DNA Mini Kit (Tissue) Geneaid. The PCR-RAPD analysis was performed using 6 primers, OPA 04, OPB 08, OPB 12, OPC 16, OPH 01, and OPH 03. The product of PCR-RAPD was electrophoresed using 1,5% agarose in 1x TAE buffer. The results showed that the similarity value of 12 local chickens using RAPD ranged from 0% to 100%. The relationship between 12 local chickens using RAPD marker indicated that the chickens in the same morphology were not clustered in the same group. This suggests that the same group of chickens phenotypically does not necessarily have the same genotype. Keywords: Local Chicken, PCR-RAPD
KEANEKARAGAMAN ANGGREK EPIFIT DI KAWASAN TAMAN WISATA ALAM DANAU BUYAN-TAMBLINGAN I Gusti Ayu Agung Pradnya Paramitha; I Gede Putu Ardhana; Made Pharmawati
Metamorfosa: Journal of Biological Sciences Vol 1 No 1 (2012)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

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Abstract

Orchids are often found growing naturally in the tropical rain forest. Destruction of tropical rain forests can reduce the germplasms of natural orchids. One of the tropical rain forest areas which is often visited by tourists in Bali is Buyan-Tamblingan Nature Tourism Park. Research conducted from December 2011 until April 2012. The results showed that in the Buyan-Tamblingan Nature Tourism Park there are 30 species of epiphyte orchids. The epiphyte orchids which have the highest individual number is Appendicula elegans Rchb. f with total individual number was 198 individuals/ha. The Shannon-Wienner diversity index value of epiphyte orchid in Buyan-Tamblingan Nature Tourism Park is 1,1561.
Monitoring Proviral DNA Of Jembrana Disease Virus In Bali Cattle Using PCR Ni Putu Eka Krisnayanti; Made Pharmawati; Inna Narayani; Ni Luh Putu Agustini
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i01.p03

Abstract

The development of biotechnology in molecular field make it possible to detect the presence of a disease earlier, faster and more accurately. One of the technique in biotechnology which has been highly developed and often be used in biological and medical research is PCR method. This molecular technique allows to accurately identify the DNA level in presence of a particular disease. This study aims to do monitoring the presence of proviral DNA Jembrana Disease Virus in bali cattle from all regencies in Bali. The blood samples of bali cattle is taken from all regencies in Bali in 2017 as many 170 samples and in 2018 from Buleleng Regency there are 101 samples and form Karangasem Regency 152 samples. DNA is extracted by QIAamp DNA blood mini kit and samples from 2017 is made to 34 pool samples, whereas samples from 2018 is made to 20 pool samples (Buleleng Regency) and 30 pool samples (Karangasem Regency). PCR is conducted by JDV 1 primer and JDV 3 primer in total reaction 25 µL with 35 cycles. PCR is also conducted on positive control and negative control. The result indicate that 34 pool samples of bali cattle from 2017 or 20 pool samples and 30 pool samples from 2018 is not indicate the presence of proviral DNA Jembrana Disease Virus. Keyword: PCR, proviral DNA, Jembrana Disease Virus
Deteksi Level Antibodi Pada Serum Darah Anjing Kintamani Pasca Vaksinasi Rabies dengan Direct ELISA I Putu Agus Tirta Cahyana; Made Pharmawati; Inna Narayani
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p19

Abstract

The success of rabies vaccination is characterized by the growth of seropositive (?0,5 IU) antibody titers after vaccination. One of the tests carried out to monitor antibody growth is ELISA (Enzyme Linked Immunosorbent Assay). This study aims to determine the effect of different vaccine doses on rabies antibody growth in kintamani dogs. The study was conducted at Perum Taman Graha, Jimbaran and Bali Besar Veteriner (BBVet) Denpasar, Bali. The design of the study used 2 variables, namely differences in vaccine doses (0.5 and 0.75 cc) and blood collection times (1, 2 and 3 months after vaccination). The results showed that vaccine dose of 0.5 cc produced higher antibody titers than vaccine dose of 0.75 cc, however it was not significantly different based on t-test. Key word : Antibody, ELISA, Rabies
Perbanyakan Begonia Bimaensis Undaharta & Ardaka Dengan Teknik Kultur Jaringan Tiwi Wati; Ida Ayu Astarini; Made Pharmawati; Ema Hendriyani
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i01.p15

Abstract

Begonia bimaensis Undaharta & Ardaka adalah salah satu Begonia yang dikembangkan di Kebun Raya ‘Eka Karya’ Bali. Jenis ini merupakan spesies endemik yang ditemukan di Gunung Muria di Kabupaten Bima, Pulau Sumbawa. Begonia bimaensis memiliki rambut putih yang tumbuh pada permukaan atas daunnya. Perbanyakan tanaman B. bimaensis perlu dilakukan dengan teknik kultur jaringan karena terbatasnya tanaman dan untuk menjaga kelestarianya. Tujuan penelitian ini adalah mengetahui teknik sterilisasi yang tepat untuk inisiasi eksplan daun B. bimaensis dan mengetahui kombinasi ZPT kinetin dan 2,4-D yang paling efektif serta pengaruhnya terhadap pertumbuhan kultur B .bimaensis. Penelitian ini menggunakan media dasar MS (Murashige dan Skoog) dengan penambahan kombinasi ZPT kinetin (0 ppm, 1 ppm, dan 2 ppm) dan 2,4- D (0 ppm, 0,5 ppm, dan 1 ppm). Percobaan terdiri dari 9 perlakuan yang setiap perlakuan terdiri dari 3 ulangan dengan tiap ulangan terdiri dari 1 botol. Hasil penelitian menunjukkan bahwa teknik sterilisasi yang tepat yaitu menggunakan kombinasi sterilan yang berbeda (sabun Sunlight, fungisida, pemutih Bayclin, dan alkohol) dan waktu perendaman menggunakan fungisida selama 30 menit. Pemberian ZPT kinetin dan 2,4-D terhadap B. bimaensis mengakibatkan pertumbuhan yang lebih cepat. Pengaruh paling tinggi terhadap pertubuhan kultur B. bimaensis diperoleh pada hari ke-12 setelah tanam dengan pemberian kinetin 2 ppm dan 2,4-D 0,5 ppm. Kata kunci: in vitro, Begonia, 2,4-D, kinetin
Struktur Populasi Ikan Tuna Mata Besar (Thunnus obesus) Dengan Analisis DNA Mikrosatelit Di Perairan Samudera Hindia Andi Bahtiar Batti; Made Pharmawati; I Gusti Ngurah Kade Mahardika
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p16

Abstract

Bigeye tuna (Thunnus obesus) is a main commodity for international markets of tuna fisheries in Indonesia, resulting in an increasing tuna capturing across Indian Ocean; Therefore, improvements in management and catchment are needed for tuna sustainability, such as a better understanding in its population structures. 30 fins tissues of bigeye tuna in total, were collected from two parts of Indian Ocean, (western Sumatra and southern Nusa Tenggara) during 20 December 2015 until Mei 2016. This study was aimed to gain some information on population structures of bigeye tuna of Indian Ocean based on microsatellite analysis. DNA analysis were conducted in a laboratory of Institute of Marine Research and Development, Gondol, Bali, Indonesia and the 1st Base -Singapore. The benefit of this study is to provide data and information on population structures of bigeye tuna of Indian Ocean. The results of Analysis of Molecular Variance (AMOVA) indicated that populations of bigeye tunas captured from the two fishing areas are included in one population.
PENGARUH MUTAGEN KIMIA SODIUM AZIDA TERHADAP MORFOLOGI TANAMAN CABAI BESAR (Capsicum annuum L.) Ni Kadek Yunita Sari; Made Pharmawati; I Ketut Junitha
Metamorfosa: Journal of Biological Sciences Vol 1 No 1 (2012)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

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Abstract

The aim of this research was to examine the effects of sodium azide at concentration of 1 mM, 2 mM, 3 mM, 4 mM and 5 mM on the morphological characters of Capsicum annuum L. The method used for the observation of morphological characters was direct observation by measuring plant height, leaf length and width as well as counting the number of leaves. The results showed that effect of sodium azide at 3 mM caused an increase in plant height, while concentration of 1 mM, 2 mM, 4 mM dan 5 mM caused a decrease in plant height. Similar trends were found for number of leaf. Leaf length and width tended to increase in sodium azide treatments.
Identification of Bacteria Caused Bacterial Wilt Disease in Potato Plants (Solanum tuberosum L.) in Taman Tanda Village, Tabanan, Bali I Komang Adi Widyastama; Retno Kawuri; Made Pharmawati
Metamorfosa: Journal of Biological Sciences Vol 10 No 2 (2023)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

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Abstract

Salah satu masalah utama pada budidaya tanaman kentang di Desa Taman Tanda adalah serangan penyakit layu bakteri yang disebabkan oleh patogen bakteri. Penyebab layu bakteri adalah bakteri dari famili Ralstoniaceae. Tujuan dari penelitian ini adalah mengidentifikasi bakter penyebab layu bakteri pada tanaman kentang di Desa Taman Tanda, Tabanan, Bali. Metode identifikasi diawali dengan isolasi patogen dari tanaman kentang yang sakit di lapangan menggunakan media selektif Ralstonia casein peptone glukose. Koloni yang tumbuh pada media selektif kemudian dikarakterisasi morfologi dan biokimia. Patogenitas dari patogen diuji dengan menginokulasi patogen pada tanaman kentang berumur 30 hari di rumah kaca. Tanaman kentang yang menunjukkan gejala layu bakteri seperti layu kering pada daun dan batang tanaman dikonfirmasi sebagai Ralstonia sp. Isolat patogen kemudian diekstrak 16S rRNA dan diamplifikasi dengan metode Polymerase Chain Reaction (PCR) menggunakan primer U1 forward dan reverse. Sekuensing 16S rRNA dilakukan satu arah dengan mengirim DNA ke PT. Genetika Science, Jakarta. Hasil sekuensing kemudian dianalisis BLASTN dengan membandingkan pusat data di GenBank. Analisis BLASTN menunjukkan isolat patogen teridentifikasi sebagai Ralstonia solanacearum strain K60-1 dengan kemiripan 100%. Dendogram dari isolat patogen dibuat dengan metode neighbor joining tree 1000 kali bootstrap. Berdasarkan identifikasi morfologi, biokimia, Postulat Koch, dan molekuler bakteri penyebab layu bakteri pada tanaman kentang di Desa Taman Tanda, Tabanan, Bali teridentifikasi sebagai Ralstonia solanacearum.
Co-Authors A. A. Putri Ardyanti A.A.S. A. Sukmaningsih Agriani Dewinta Anak Agung Ayu Putri Ardyanti Anak Agung Gde Raka Dalem Andi Bahtiar Batti Andrianus Sembiring, Andrianus Aziz, Alifya Ibnu Bayu Adjie Bayu Adjie Bayu Adjie Bram Setyadji Deny Suhermawan Yusup Dian Catur Prayantini Dwi Nur Rikhma Sari Eka Fibayani Imaniar Eka Fibayani Imaniar Ema Hendriyani Fransiska Nitti Fransiskus Jimmy Roga Gde Evayanti, Luh GUIJUN YAN Gusti Ayu Putri Arnila I Gde Suryadi I Gede Putu Ardhana I Gusti Agung Eka Saraswati I Gusti Ayu Agung Pradnya Paramitha I Gusti Ngurah Kade Mahardika I Ketut Catur Wiguna I Ketut Junitha I Ketut Suada I Komang Adi Widyastama I MADE AGUS WIARTANA I Made Anom Sutrisna Wijaya I Made Murna I Putu Agus Tirta Cahyana I Putu Satwika I Wayan Arnata I Wayan Rosiana I. Setyawati I.G.A.S. Wahyuni I.M.A.S. Wijaya IAN JAMES MACFARLANE IDA AYU ASTARINI Ida Ayu Ratih Purnama Dewi Imaniar, Eka Fibayani Indah Tria Hoky Inna Narayani IRIANI SETYAWATI Joko Raharjo, Sentot Junita Hardini Ketut Queena Fredlina Luh Putu Wrasiati Made Ria Defiani Maharani, Rai Janaki Maliza Kurnia Maya Agustina Maya Agustina Mery Wintari Ngurah Intan Wiratmini Ni Kadek Dewi Rustini NI KADEK YUNITA SARI Ni LUH ARPIWI Ni Luh Ayu Jami Wistiani, Ni Luh Ayu Jami Ni Luh Putu Agustini Ni Luh Watiniasih Ni Luh Wayan Yulia Mirayanti NI MADE DIAN PRATIWI Ni Made Gari Ni Made Sastriyani Wiendra Ni Made Sastriyani Wiendra Ni Made Suartin Ni Nyoman Wirasiti Ni Putu Adriani Astiti Ni Putu Eka Krisnayanti Ni Wayan Ayu Wiartini Ni Wayan Karolina Ni Wayan Sudatri PATRICK FINNEGAN Putera, I Kadek Aridena Putu Angga Wiradana Putu Apriliani Putu Yunita Putri Aryani Retno Kawuri Rifky Firmansyah Anwar Satwika, I Putu Septarini Dian Anitasari Septarini Dian Anitasari Siddique, Kadambot.H.M. Tassanai Jaruwatanaphan Tassanai Jaruwatanaphan Tassanai Jaruwatanaphan, Tassanai Tiwi Wati Uslan UUL SHOVI NURKAMILA Vanesa Martida Vanesa Martida Viryanando Evan Rahardja WAHYU WIDOWATI Wawan Sujarwo WENNI SETYO LESTARI Wenni Setyo Lestari Wenni Setyo Lestari, Wenni Setyo Wijaya, Putu Austin Widyasari Wildan Mujahidul Basyar Yasuyuki Watano Yasuyuki Watano Yasuyuki Watano, Yasuyuki Yenni Ciawi Zaetuna, Hanifa Laila