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All Journal Jurnal Adabiyah Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Kiprah Journal of Education, Humaniora and Social Sciences (JEHSS) PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Entomologi Indonesia ACTIVE: Journal of Physical Education, Sport, Health and Recreation Pepatudzu : Media Pendidikan dan Sosial Kemasyarakatan BIMIKI (Berkala Ilmiah Mahasiswa Ilmu Keperawatan Indonesia) GANDRUNG: Jurnal Pengabdian Kepada Masyarakat Journal of Innovation in Educational and Cultural Research Jurnal Riset dan Inovasi Peternakan (Journal of Research and Innovation of Animals) Jurnal Ekonomi Trend : Pemasaran, Produksi, Keuangan, SDM, Akuntansi dan Sistem Informasi Manajemen Indonesian Journal of Laboratory Prima Abdika: Jurnal Pengabdian Masyarakat International Journal of Community Service Journal of Experimental and Clinical Pharmacy (JECP) Journal Peqguruang: Conference Series Jurnal Pengabdian Kepada Masyarakat Patikala Indo-MathEdu Intellectuals Journal Shautuna: Jurnal Ilmiah Mahasiswa Perbandingan Mazhab JURNAL PABEAN : PERPAJAKAN BISNIS EKONOMI AKUNTANSI MANAJEMEN Makara Journal of Science JURNAL SIPISSANGNGI: Jurnal Pengabdian Kepada Masyarakat Berita Kedokteran Masyarakat Kompeten: Jurnal Ilmiah Ekonomi dan Bisnis Indonesian Journal of Health Information Management Services (IJHIMS) Innovative: Journal Of Social Science Research Biner : Jurnal Ilmu Komputer, Teknik dan Multimedia JRIIN :Jurnal Riset Informatika dan Inovasi Khidmat : Jurnal Pendidikan dan Ilmu Sosial Muhibbul Arabiyah: Jurnal Pendidikan Bahasa Arab Mammiri: Jurnal Pengabdian Masyarakat Efisiensi: Jurnal Penelitian dan Pengembangan Ilmu Manajemen
Ayu Rahayu
Fakultas Peternakan Universitas Jenderal Soedirman, Purwokerto
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Transportation Other

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Integrating Next Generation Science Standards Dimensions in Preparing Prospective Biology Teachers Oriented to Traditional Food Culture Sukmawati, Ika; Rahayu, Ayu; Alamsyah, Muhammad Radian Nur
Journal of Innovation in Educational and Cultural Research Vol 7, No 3 (2026): Article in Press
Publisher : Yayasan Keluarga Guru Mandiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46843/jiecr.v7i3.2974

Abstract

This study examines the declining interest among prospective biology teachers in traditional food culture, which is essential to supporting sustainable development goals, particularly food security and environmental sustainability. The research aims to develop a learning model integrating the Next Generation Science Standards (NGSS) dimensions with traditional food culture, known as the Traditional Food Culture Learning (TFCL) model. A research and development (RD) method based on the Dick and Carey instructional design was employed, involving prospective biology teachers at Universitas Tidar. The model was developed through stages of needs analysis, design, validation by multidisciplinary experts, and revision. The results indicate that the TFCL model is feasible and effective, with validation scores indicating very strong performance. The model's implementation shows significant positive relationships among exploration of local knowledge, nutritional analysis skills, and student engagement. The study concludes that the TFCL model enhances teaching competencies and fosters awareness of traditional food culture. This research contributes to science education by providing an innovative, culturally contextualized learning model that integrates global standards with local wisdom to promote sustainability-oriented learning.
GAMICBANK: Pendekatan Inovatif dalam Pelestarian Isolat Mikroba Mulyani, Mulyani; Awaludin, Kamla; Rahayu, Ayu; Nuryastuti, Titik
Indonesian Journal of Laboratory Vol 9, No 1 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijl.v9i1.112540

Abstract

ABSTRAK. Pelestarian isolat mikroba sangat penting untuk penelitian, diagnostik, dan bioteknologi. Metode konvensional seperti stok gliserol atau liofilisasi efektif namun seringkali mahal dan rentan terhadap risiko kontaminasi. Strategi alternatif penyimpanan berbasis butiran, memungkinkan pengambilan mikroorganisme yang masih hidup dengan mudah sambil meminimalkan siklus pembekuan-pencairan berulang. Studi ini bertujuan untuk mengevaluasi kinerja awal sistem GAMICBANK, metode penyimpanan mikroba berbasis butiran menggunakan butiran mikrobank plastik yang dimasukkan dalam larutan susu skim atau Skim Milk–Tryptone–Glucose–Glycerol (STGG), untuk menyimpan bakteri dan ragi pada suhu –80 °C. Isolat Gram-positif, Gram-negatif, dan ragi yang representatif (Staphylococcus aureus, Streptococcus spp., Listeria monocytogenes, Salmonella typhi, Shigella flexneri, Escherichia coli, Neisseria gonorrhoeae, Chromobacterium violaceum, dan Candida albicans) disimpan dalam butiran dengan susu skim atau STGG. Butiran tersebut diambil kembali setelah 1 dan 3 bulan, ditanam pada media selektif, dan dievaluasi pertumbuhan koloni dan viabilitasnya. Setelah 1 bulan, semua isolat tetap memiliki viabilitas yang sangat baik di kedua media. Pada 3 bulan, sebagian besar spesies tetap sangat viabel (>99%) di kedua media. Namun, Listeria monocytogenes dan Neisseria gonorrhoeae menunjukkan penurunan viabilitas yang signifikan dalam susu skim (30%) tetapi tetap stabil dalam STGG (99%). Demikian pula, Candida albicans menunjukkan penurunan viabilitas dalam susu skim (50%) tetapi mempertahankan 99% kelangsungan hidup dalam STGG. Sistem bead GAMICBANK secara efektif melestarikan mikroorganisme yang tangguh dan sensitif pada suhu –80 °C. STGG memberikan perlindungan beku yang lebih unggul dibandingkan susu skim, terutama untuk bakteri sensitif dan ragi. Kesimpulannya bahwa sistem GAMICBANK dapat dijadikan sebagai metode alternatif untuk penyimpanan isolat bakteri dan ragi.ABSTRACK.The preservation of microbial isolates is crucial for research, diagnostics, and biotechnology. Conventional methods, such as glycerol stocks or lyophilization, are effective but frequently expensive and susceptible to contamination risks. An alternative strategy, bead-based storage, facilitates the easy retrieval of viable microorganisms while minimizing repeated freeze-thaw cycles. This study aims to evaluate the initial performance of the GAMICBANK system, a bead-based microbial storage method utilizing plastic microbank beads submerged in either skim milk or Skim Milk–Tryptone–Glucose–Glycerol (STGG) solutions, for the cryopreservation of bacteria and yeast at –80 °C. Representative Gram-positive, Gram-negative, and yeast isolates (Staphylococcus aureus, Streptococcus spp., Listeria monocytogenes, Salmonella typhi, Shigella flexneri, Escherichia coli, Neisseria gonorrhoeae, Chromobacterium violaceum, and Candida albicans) were preserved in beads using either skim milk or STGG. The beads were retrieved after 1 and 3 months of storage, cultured on selective media, and evaluated for colony growth and viability. After 1 month, all isolates maintained excellent viability in both preservation media. At 3 months, the majority of the species remained highly viable (>99%) in both media. However, Listeria monocytogenes and Neisseria gonorrhoeae exhibited a significant decline in viability when stored in skim milk (30%) but remained stable in STGG (99%). Similarly, Candida albicans demonstrated a reduction in viability in skim milk (50%) while maintaining a 99% survival rate in STGG. The GAMICBANK bead system effectively preserves both robust and sensitive microorganisms at –80 °C. STGG provides superior cryoprotection compared to skim milk, particularly for sensitive bacteria and yeast. In conclusion, the GAMICBANK system serves as a viable alternative method for the preservation of bacterial and yeast isolates. 
Co-Authors 'Aqobah, Qory Jumrotul Abdul Latif Abdul Malik Abicandra, Muhammad Nidhom Hamami Adewia, Musdalifatul Adi Utarini Agus Nasir, Agus Agus Susanto Ahmad Gunawan Ahmad Zaki Akbar, Aidul Alamsyah, Muhammad Radian Nur ANDRIA, YOGI Anugrah, Sendy Mohamad Ardhana, Argi Yhudin Avri Arfandi Adnan, Mohammad Arifin, Rukminih Asdar Asdar Assaibin, Muhammad Awaluddin Awaludin, Kamla Basri Basri Burhan, Imron Citra Indriani Daniel Happy Putra Daniwijaya, Edwin Widyanto Daspar Daspar DIAN ARUNI KUMALAWATI Domas Fitria Widyasari Dwi Budi Santoso Eggi Arguni Eka Kartika, Andi Febryanti, Febryanti Fiqran, Fiqhi Al Fitri Fitri Fitriana, Iva Fitriyaningrum, Rista Indah Geerhand, Andi Gunawan Gunawan Haeruddin Haeruddin Haryono, Wasis Hasan, Muhammad Hafiz Herlina Ahmad, Herlina Huriati, Huriati Ika Sukmawati, Ika Ilmi, Ani Auli Ishak, Suryadi Jumrotul 'Aqobah, Qory Khadijah Khadijah Khifa, Mastura Kurniasari, Irianti Lena Suryani K, Dewi Liani, Ahyani Mirah Luthfiana, Nadia Ade Mardian Mardian Masnur Ali Miftahul Jannah Muhammad Dima Iqbal Hamdani, Muhammad Dima Iqbal Muin, Rahma Mulyani Mulyani Nasir, Norma Nindiani, Aisah Nur Asia, Nur Nurul Afifah Nuryadin, Arief Ocktadinata, Robbi Oktaviani Naulita Turnip Patima, Patima Perani Rosyani Pramuko, Nida Budiwati Purnomo, Galih Santo Puspitasari, Serli Qory Jumrotul Aqobah Qudratullah, Qudratullah Rahayu, Enik Rahmadani, Fatwa Suci Ratu Diah Koerniawati Reny, Reny Rian Triprayogo Rio Hermantara Riris Andono Ahmad Rizky, Azmiyansyah Rosti, Rosti Rovik, Anwar Sahabuddin, Chuduriah Saraswati, Utari Setiyadi, Sina Setya Agus Santosa Siti Aisyah Sopiandy, Dede Sry Astuti, Sry Sukawan, Ari Sukmawati Sukmawati Supriyati, Endah Sutamrin Syah, Ahmad Syamsuddin Syamsuddin Syazeedah, Haudli Nurfitrah Uskytia Titik Nuryastuti Turnip, Oktaviani Naulita Wardana, Dwi Satria Warsito Tantowijoyo, Warsito Wazithah , Mar Athul Wulandari, Erli Yasmun, Andi Nurunnisa Yulianti Pratiwi, Danisya Yusran Yusran Zahra, Titis Wahyu Zamzani, Nurul