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Journal : INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY

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The effects of varying in incubation time and temperature of methyl salicylate as a clearing agent on the quality of breast tissue slides Fathiasari, Fariha; Wiryanti, Wiwin; Durachim, Adang; Rahmat, Mamat
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 2 (2024): Promising and valuable research towards diagnosis, prognosis and treatment of dis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i2.4812

Abstract

Non-polar clearing agents have the ability to dissolve breast adipose tissue. While fat dissolution occurs during the clearing process, careful monitoring of adipose cell membranes mis crucial due to their impact on the behavior of breast cancer cells and disease progression. Xylol is a widely employed clearing agent. However, its toxicity and prolonged clearing time necessitate the exploration of alternatives. In this context, methyl salicylate emerges as a viable substitute. It is non-polar nature facilitates efficient fat dissolution, leading to quicker tissues clarification. This study adopted a quasi-experimental method, utilizing 24 pieces of breast tissue as the sample. These tissue sample were divided into six groups according to the treatment regimen. The treatment groups involved breast tissue clearing using xylol for 2x60 minutes at room temperature, methyl salicylate for 2x30 minutes at room temperature, and methyl salicylate at 60°C for 20 minutes, 30 minutes, 2x20 minutes, and 2x30 minutes. The quality of breast tissue slides was assessed by analyzing the color contrast between the nucleus and cytoplasm using ImageJ software, along with the clarity of adipose cell cell membrane using microscopy. The results indicated that prolonged exposure at high temperature resulted in poor quality breast tissue slides. Conversely, tissue clearing with methyl salicylate for 2x30 minutes at room temperature exhibited excellent contrast between the nucleus and cytoplasm, as well as clear adipose cell membranes. Further study is warranted to explore the applicability of methyl salicylate as a clearing agent in tissues with lower fat content.
Co-Authors Adelia Sapitri Agus Sulaeman Ai Nuni Risnawati Alfa Rohmatin Alimuddin Unde, Alimuddin Amanah, Ninda Risti Andrafikar, Andrafikar Andriawan Andriawan Anton Royanto Ahmad Berlian, Selpia Cep Wawan Dandi Sanjaya Dermawan, Asep Dewi Nurmuslihat Durachim, Adang Dwitantika, Bryan Dzil Ikrom, Fadhli Edi, Sutoyo Elfrianto, Kevin Elis Ashpiati Evi Mulyati Fadhli Dzil Ikrom, Fadhli Dzil Fahrudin, Ade Faisal, Ardian Faizal, Muhamad Nur Faried, Muhamad Fathiasari, Fariha Fauziyah, Raden Roro Nur Fauziyah, Roro Nur Feisyal Rinaldi, Sonny Firdaus, Fahdina Fred Agung Suprihartono, Fred Agung Fuji Lestari Ghaisani, Syifa Marha Ghassani, R Wulandari gumilar, mulus Hablinur Alkindi Hafied Cangara, Hafied Hardi, Zon Hastuti, Widi Hayati, Eem Ihsan Ihsan Iis Kurniati Ilmi Sufa, Hafizah Isdiany, Nitta Judiono Judiono Kamaruddin, Imran Kawaitouw, Leberina Khasanah, Nunung Dwi Nur Kusmawan, Rudi Latipah Latipah Maemunah, Iin MAHMUDAH, UMMI Maryati Dewi, Maryati Mohamad Erihadiana, Mohamad Mona Fitria Mufidah, Zahra Muhamad Iqbal Muhammad Ridwan Mulia, Yuliansyah Sundara N Alfiyah Najiyyah, Amalia Nur Nidianti, Della Nina Marliana, Nina Noriko Sato Nur Fitriani, Melia Nur Indra, Asep Iin Nuraini, Aisyah Iadha Nurdianto, Gian Nurhakim, Haditsa Qurani Nurrohman Nurrohman Paoz Mutaqin Pujiawaati Pujiawaati Purnawan, Asep Iwan Pusparini Pusparini Rachmansyah, Yoki Ridwanna, Surya Risa Rahmawati, Risa rohayati rohayati Rohili, Ilyas Rohmah, Andini Aulia Roy Waluyo Ruswandi, Irwan Saleky, Yohannes Willihelm Setya Permana Sutisna Setyawan, Gigih Bayu Shah, Syed Humayon Sholihah, Siti Nurbayani Simabur, Lisda Ariani Siti Dandawiah Siti Rafilda Maldah siti rohilah Siti Rohimah Siti Sarah Nurfadilah Sopian, Yusuf Subagio, Rohmad Sufa, Hafizah Ilmi Sulaeman Sulaeman Sumarjo, Dwi Nur Ida Islamiati Suparman Suparman Sutoyo, Edi Syaindi, Amanda Afifah Takahiro F. Tika Hafzara Siregar Tsania, Nanda Umi Mahmudah Widartika Widartika Wiratmoko, Aris Wiryanti, Wiwin Witri Priawantiputri Zuri Rismiarti