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Journal : Current Biomedicine

Efek penggunaan ulang larutan fiksatif formalin pada kualitas preparat histopatologi dan jumlah limbah yang dihasilkan Hardi, Zon; Wiryanti, Wiwin; Durachim, Adang; Rahmat, Mamat
Current Biomedicine Vol. 2 No. 2 (2024): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.2.2.71-83

Abstract

Background: Neutral buffered formalin (NBF) 10% fixative solution is widely used in histopathological slides. The fixation process generates liquid waste of NBF 10% and solid waste of tissue remnants. Objective: The research aimed to assess the reuse of NBF 10% fixative solution on the quality of histopathological slides and calculate the amount of waste produced. Methods: Treatments included single-use of fixative solution (control), reuse for 1, 2, and 3 times. Ten sample slides were prepared for each treatment, consisting of intestinal tissue, uterine fibroids, prostate, uterus, ovarian cyst, portio vaginalis cervicis, thyroid, rectum, breast fibroadenoma, and gallbladder tissues. Tissues were fixed with NBF 10% and processed histologically with hematoxylin-eosin staining. Liquid waste of NBF 10% and solid waste of tissue remnants were quantified. Histopathological slide quality was measured under a microscope for nuclear and cytoplasmic clarity, staining intensity, and color uniformity. Results: Control slides exhibited good quality with clearly blue-stained nuclei, pink cytoplasm, no color accumulation, and uniform staining across fields of view. Reused NBF 10% slides experienced a decrease in quality compared to the control but were still usable for diagnosis. Slides reused 2 and 3 times showed poor quality, making diagnosis difficult. Fixation resulted in 299.0 liters of liquid waste of NBF 10% and 64.9 kilograms of solid tissue remnants. Conclusion: Reusing NBF 10% decreases histological slide quality, though reuse once still allows for diagnosis. Reusing 10% NBF for tissue fixation can reduce the liquid waste of fixative solution and solid tissue waste.
Co-Authors Adelia Sapitri Agus Sulaeman Ai Nuni Risnawati Alfa Rohmatin Alimuddin Unde, Alimuddin Amanah, Ninda Risti Andrafikar, Andrafikar Andriawan Andriawan Anton Royanto Ahmad Berlian, Selpia Cep Wawan Dandi Sanjaya Dermawan, Asep Dewi Nurmuslihat Durachim, Adang Dwitantika, Bryan Edi, Sutoyo Elfrianto, Kevin Elis Ashpiati Elysia, Syahda Evi Mulyati Fadhli Dzil Ikrom Fahrudin, Ade Faisal, Ardian Faizal, Muhamad Nur Faried, Muhamad Fathiasari, Fariha Fauziyah, Raden Roro Nur Fauziyah, Roro Nur Feisyal Rinaldi, Sonny Firdaus, Fahdina Fred Agung Suprihartono, Fred Agung Fuji Lestari Ghaisani, Syifa Marha Ghassani, R Wulandari gumilar, mulus Hablinur Alkindi Hafied Cangara, Hafied Hardi, Zon Hastuti, Widi Hayati, Eem Ihsan Ihsan Iis Kurniati Ilmi Sufa, Hafizah Imran Kamaruddin Isdiany, Nitta Judiono Judiono Kawaitouw, Leberina Khasanah, Nunung Dwi Nur Kusmawan, Rudi Latipah Latipah Maemunah, Iin MAHMUDAH, UMMI Maryati Dewi, Maryati Mohamad Erihadiana, Mohamad Mona Fitria Mufidah, Zahra Muhamad Iqbal Muhammad Ridwan Mulia, Yuliansyah Sundara N Alfiyah Najiyyah, Amalia Nur Nidianti, Della Nina Marliana, Nina Noriko Sato Nur Fitriani, Melia Nur Indra, Asep Iin Nuraini, Aisyah Iadha Nurdianto, Gian Nurhakim, Haditsa Qurani Nurrohman Nurrohman Paoz Mutaqin Pujiawaati Pujiawaati Purnawan, Asep Iwan Pusparini Pusparini Rachmansyah, Yoki Ridwanna, Surya Risa Rahmawati, Risa rohayati rohayati Rohili, Ilyas Rohmah, Andini Aulia Roy Waluyo Ruswandi, Irwan Saleky, Yohannes Willihelm Setya Permana Sutisna Setyawan, Gigih Bayu Shah, Syed Humayon Sholihah, Siti Nurbayani Simabur, Lisda Ariani Siti Dandawiah Siti Rafilda Maldah siti rohilah Siti Rohimah Siti Sarah Nurfadilah Sopian, Yusuf Subagio, Rohmad Sufa, Hafizah Ilmi Sulaeman Sulaeman Sumarjo, Dwi Nur Ida Islamiati Suparman Suparman Sutoyo, Edi Syaindi, Amanda Afifah Takahiro F. Tika Hafzara Siregar Tsania, Nanda Umi Mahmudah Widartika Widartika Wiratmoko, Aris Wiryanti, Wiwin Witri Priawantiputri Zuri Rismiarti