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Journal : Mukhtabar: Journal of Medical Laboratory Technology

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Effect of Variation Natrium Sitrat Concentration and Centrifugation Time on Prothrombin Time (PT) Value Najiyyah, Amalia Nur; Marliana, Nina; Hayati, Eem; Rahmat, Mamat
Mukhtabar Journal of Medical Laboratory Technology Vol 2 No 2 (2024): Mukhtabar: Journal of Medical Laboratory Technology (October 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v2i2.694

Abstract

Background & Objective: Prothrombin Time (PT) test is one of the hemostasis tests used to determine whether there is a deficiency of extrinsic clotting factors and co-pathway clotting factors, namely factors I, II, V, VII, and X. The pre-analytical stage that must be considered in the hemostasis examination is the concentration of anticoagulant used and the centrifugation process. This study was conducted to determine the effect of variations in sodium citrate anticoagulant concentration and centrifugation time on Prothrombin Time (PT) values.  Method: The anticoagulants used in this study were 3.2% and 3.8% sodium citrate. Centrifugation is the process of separating cells from plasma. The effect of improper centrifugation is to increase the number of platelets, so that platelet factors contained in platelets will accelerate the formation of fibrin. The research conducted is a pseudo-experimental study by giving treatment to blood specimens that will be added with anticoagulant Sodium Citrate concentrations of 3.2% and 3.8% with a blood ratio of 9:1, then centrifuged at 3000 rpm and given a variation of time for 15 minutes, 10 minutes, and 5 minutes. The results were tested using Saphiro Wilk and Friedman Test. Result : Based on the research that has been done, the Asymp. Sig. value obtained in the Friedman Test is >0.05. This indicates that the treatment that has been given to the sample has no effect on the value of Prothrombin Time. Conclusion: It can be concluded that there is no significant difference between the variation of sodium citrate concentrations of 3.2% and 3.8%, as well as in the variation of 3000 rpm centrifugation time of 15 minutes, 10 minutes, and 5 minutes on the value of Prothrombin Time (PT).
Co-Authors Adelia Sapitri Agus Sulaeman Ai Nuni Risnawati Alfa Rohmatin Alimuddin Unde, Alimuddin Amanah, Ninda Risti Andrafikar, Andrafikar Andriawan Andriawan Anton Royanto Ahmad Berlian, Selpia Cep Wawan Dandi Sanjaya Dermawan, Asep Dewi Nurmuslihat Durachim, Adang Dwitantika, Bryan Edi, Sutoyo Elfrianto, Kevin Elis Ashpiati Elysia, Syahda Evi Mulyati Fadhli Dzil Ikrom Fahrudin, Ade Faisal, Ardian Faizal, Muhamad Nur Faried, Muhamad Fathiasari, Fariha Fauziyah, Raden Roro Nur Fauziyah, Roro Nur Feisyal Rinaldi, Sonny Firdaus, Fahdina Fred Agung Suprihartono, Fred Agung Fuji Lestari Ghaisani, Syifa Marha Ghassani, R Wulandari gumilar, mulus Hablinur Alkindi Hafied Cangara, Hafied Hardi, Zon Hastuti, Widi Hayati, Eem Ihsan Ihsan Iis Kurniati Ilmi Sufa, Hafizah Imran Kamaruddin Isdiany, Nitta Judiono Judiono Kawaitouw, Leberina Khasanah, Nunung Dwi Nur Kusmawan, Rudi Latipah Latipah Maemunah, Iin MAHMUDAH, UMMI Maryati Dewi, Maryati Mohamad Erihadiana, Mohamad Mona Fitria Mufidah, Zahra Muhamad Iqbal Muhammad Ridwan Mulia, Yuliansyah Sundara N Alfiyah Najiyyah, Amalia Nur Nidianti, Della Nina Marliana, Nina Noriko Sato Nur Fitriani, Melia Nur Indra, Asep Iin Nuraini, Aisyah Iadha Nurdianto, Gian Nurhakim, Haditsa Qurani Nurrohman Nurrohman Paoz Mutaqin Pujiawaati Pujiawaati Purnawan, Asep Iwan Pusparini Pusparini Rachmansyah, Yoki Ridwanna, Surya Risa Rahmawati, Risa rohayati rohayati Rohili, Ilyas Rohmah, Andini Aulia Roy Waluyo Ruswandi, Irwan Saleky, Yohannes Willihelm Setya Permana Sutisna Setyawan, Gigih Bayu Shah, Syed Humayon Sholihah, Siti Nurbayani Simabur, Lisda Ariani Siti Dandawiah Siti Rafilda Maldah siti rohilah Siti Rohimah Siti Sarah Nurfadilah Sopian, Yusuf Subagio, Rohmad Sufa, Hafizah Ilmi Sulaeman Sulaeman Sumarjo, Dwi Nur Ida Islamiati Suparman Suparman Sutoyo, Edi Syaindi, Amanda Afifah Takahiro F. Tika Hafzara Siregar Tsania, Nanda Umi Mahmudah Widartika Widartika Wiratmoko, Aris Wiryanti, Wiwin Witri Priawantiputri Zuri Rismiarti