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Journal : Mukhtabar: Journal of Medical Laboratory Technology

 1 
Effect of Variation Natrium Sitrat Concentration and Centrifugation Time on Prothrombin Time (PT) Value Najiyyah, Amalia Nur; Marliana, Nina; Hayati, Eem; Rahmat, Mamat
Mukhtabar Journal of Medical Laboratory Technology Vol 2 No 2 (2024): Mukhtabar: Journal of Medical Laboratory Technology (October 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v2i2.694

Abstract

Background & Objective: Prothrombin Time (PT) test is one of the hemostasis tests used to determine whether there is a deficiency of extrinsic clotting factors and co-pathway clotting factors, namely factors I, II, V, VII, and X. The pre-analytical stage that must be considered in the hemostasis examination is the concentration of anticoagulant used and the centrifugation process. This study was conducted to determine the effect of variations in sodium citrate anticoagulant concentration and centrifugation time on Prothrombin Time (PT) values.  Method: The anticoagulants used in this study were 3.2% and 3.8% sodium citrate. Centrifugation is the process of separating cells from plasma. The effect of improper centrifugation is to increase the number of platelets, so that platelet factors contained in platelets will accelerate the formation of fibrin. The research conducted is a pseudo-experimental study by giving treatment to blood specimens that will be added with anticoagulant Sodium Citrate concentrations of 3.2% and 3.8% with a blood ratio of 9:1, then centrifuged at 3000 rpm and given a variation of time for 15 minutes, 10 minutes, and 5 minutes. The results were tested using Saphiro Wilk and Friedman Test. Result : Based on the research that has been done, the Asymp. Sig. value obtained in the Friedman Test is >0.05. This indicates that the treatment that has been given to the sample has no effect on the value of Prothrombin Time. Conclusion: It can be concluded that there is no significant difference between the variation of sodium citrate concentrations of 3.2% and 3.8%, as well as in the variation of 3000 rpm centrifugation time of 15 minutes, 10 minutes, and 5 minutes on the value of Prothrombin Time (PT).
Co-Authors Agus Sulaeman Alimuddin Unde, Alimuddin Amanah, Ninda Risti Andrafikar, Andrafikar Berlian, Selpia Dandi Sanjaya Dermawan, Asep Durachim, Adang Dwitantika, Bryan Dzil Ikrom, Fadhli Elfrianto, Kevin Fadhli Dzil Ikrom, Fadhli Dzil Fahrudin, Ade Faisal, Ardian Faried, Muhamad Fathiasari, Fariha Fauziyah, Roro Nur Feisyal Rinaldi, Sonny Firdaus, Fahdina Ghaisani, Syifa Marha Ghassani, R Wulandari gumilar, mulus Hafied Cangara, Hafied Hardi, Zon Hastuti, Widi Hayati, Eem Iis Kurniati Ilmi Sufa, Hafizah Isdiany, Nitta Kamaruddin, Imran Kawaitouw, Leberina Kusmawan, Rudi Maemunah, Iin MAHMUDAH, UMMI Maryati Dewi, Maryati Mohamad Erihadiana, Mohamad Mona Fitria Mufidah, Zahra Muhamad Iqbal Mulia, Yuliansyah Sundara Najiyyah, Amalia Nur Nidianti, Della Nina Marliana, Nina Noriko Sato Nur Fitriani, Melia Nur Indra, Asep Iin Nurdianto, Gian Nurhakim, Haditsa Qurani Nurrohman Nurrohman Purnawan, Asep Iwan Pusparini Pusparini Ridwanna, Surya Risa Rahmawati, Risa rohayati rohayati Rohili, Ilyas Rohmah, Andini Aulia Roy Waluyo Ruswandi, Irwan Saleky, Yohannes Willihelm Setya Permana Sutisna Simabur, Lisda Ariani Subagio, Rohmad Sufa, Hafizah Ilmi Sulaeman Sulaeman Sumarjo, Dwi Nur Ida Islamiati Suparman Suparman Sutoyo, Edi Syaindi, Amanda Afifah Takahiro F. Tsania, Nanda Umi Mahmudah Widartika Widartika Wiratmoko, Aris Wiryanti, Wiwin Witri Priawantiputri Zuri Rismiarti