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All Journal International Journal of Public Health Science (IJPHS) Journal of Food and Pharmaceutical Science Pharmaciana: Jurnal Kefarmasian Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Indonesian Journal of Pharmaceutical Science and Technology Jurnal Ilmu Farmasi dan Farmasi Klinik (Journal of Pharmaceutical Science and Clinical Pharmacy) Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY Science and Technology Indonesia Ilmu Gizi Indonesia Indonesian Journal of Chemistry JPSCR : Journal of Pharmaceutical Science and Clinical Research Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Jurnal Ilmu Kefarmasian Indonesia Jurnal Mandala Pharmacon Indonesia JURNAL FARMASIMED (JFM) Indonesian Journal of Halal Research Journal of halal product and research (JHPR) Community Empowerment Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan JURNAL INOVASI DAN PENGABDIAN MASYARAKAT INDONESIA JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Pharmaceutical Sciences and Research (PSR) Jurnal Farmasi Sains dan Praktis Jurnal Kajian Islam Modern Journal of Halal Science and Research Open Community Service Journal Journal of Pharmaceutical and Sciences. Riwayat: Educational Journal of History and Humanities Journal of Food and Pharmaceutical Sciences Medical Sains : Jurnal Ilmiah Kefarmasian Journal of Pharmacy and Halal Studies (JPHS)
Nina Salamah
Fakultas Farmasi, Universitas Ahmad Dahlan
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Journal : JURNAL FARMASIMED (JFM)

 1 
Antioxidant Activity Test of Black Rice (Oryza sativa L. Indica) Extract and Total Phenolic Test of Soybean Sprout (Glycine max L.) Extract Using 70%, 90%, and 96% Ethanol Variations. Maulizah, Rizlah; Kintoko; Nina Salamah; Elfi Anis Saati
Jurnal FARMASIMED (JFM) Vol 8 No 2 (2026): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/v5bg4116

Abstract

Background: The human body requires external antioxidants to neutralize free radicals that cause cellular damage and degenerative diseases. Black rice contains higher anthocyanin levels than red rice, while soybean germination increases flavonoids, phenolic compounds, and secondary metabolites.Objective: This study aimed to determine the total phenolic content of germinated soybeans extracted using three ethanol solvent concentrations (70%, 90%, and 96%) and to valuate the antioxidant activity of soybean and black rice extracts as copigmentation agents. Methods: Soybean extracts were obtained by maceration using different ethanol concentrations, followed by analysis of total phenolic content and antioxidant activity. Black rice extract was evaluated for antioxidant activity using the DPPH method to obtain a stable antioxidant with a very strong IC₅₀ value. Results: The total phenolic content of germinated soybean extracts varied with solvent concentration: ethanol 70% yielded 2.24 mg GAE/g, ethanol 90% yielded 7.14 mg GAE/g, and ethanol 96% yielded 3.36 mg GAE/g. The total anthocyanin content of black rice extract determined by the pH differential method was 8.182 mg/100 g, with an antioxidant activity IC₅₀ of 82.24 ± 3.6 ppm, categorized as strong. The IC₅₀ value of the non-copigmented sample was 83.71 ± 1.6 ppm (moderate), while copigmentation ratios of 1:1, 1:2, and 1:3 resulted in IC₅₀ values of 70.83 ± 0.8 ppm (strong), 62.97 ± 1.2 ppm (strong), and 38.53 ± 0.3 ppm (very strong), respectively. Conclusion: Extraction of germinated soybeans using ethanol 90% significantly increased total phenolic content (p < 0.05). Copigmentation of black rice anthocyanin flavylium with germinated soybean polyphenols at a 1:3 ratio produced very strong antioxidant activity (p < 0.05), indicating its potential as a copigmentation agent in functional food products.
Co-Authors A Riyanto Abdul Rohman Achmad Wahyudi Agustin LN Aminin Ajeng Dian Pertiwi, Ajeng Dian Andini, Prasti Anggita Devi Anita Wening Sejati Anjani, Ratna Annta Kern Nugrohowati Anwar, Muslih Any Guntarti Any Guntarti Any Guntarti Any Guntarti Any Guntarti Ar Rahma, Nur Aulia Banundari Rachmawati Barokati Azizah Basuki, Nazih Candra, Novita Diana Ayu Citra Ariani Edityaningrum, Citra Citra Dhea Cantika Danang Prasetyaning Amukti Desti Kameliani Dian Prasasti Dwi Fitriani Elfi Anis Saati Erlinda Widyasari Fathurohman SW, Oman Fathurrohman SW, Oman FATMAWATI, ARUM Fatriansari, Asih Fella Qulsum Maulida Firdaus, Anisa Firdaus, Rizqi Amalia Fitrah Amelia Gunawan Anugra Sumule, Joshua Guntarti , Any Hardi Astuti Witasari Hari Susanti Hayati, Farida Hikmah, Nazila Nur Ikawati, Retty Indah Pratami, Ria INNAYAH IZATI Irfan, Nawwar Ismarti Ismarti, Ismarti Jaswir, Irwandi Jufri, Sayyidah Luthfiyah Justitia Cahyani Fadli Juwitaningtyas, Titisari Kaffah, Silmi karunia nining handaningrum Kasmawati, Henny Kintoko Kintoko Kintoko Kurniaty, Santyas Kurniawan, Muhammad Fariez Laela Hayu Nurani Liani Farahana Ma'ruf, Muhammad Mae Sri Hartati Wahyuningsih Mahendra, Muhammad Reza Masyithah Awaluddin, Sitti Maulizah, Rizlah Miftahul Rozak Mohamed, Farahidah Muhammad Sulkhan Muhti Al Abror Mukti Utomo, Adhitya Ilham mukti, adhitya ilham Mulyaningisih, Sri Mustofa Ahda Nanik Sulistyani Nining Sugihartini Novitasari, Putri Rachma Nurkhasanah , Nurkhasanah Nurkhasanah Mahfudh Pangaribuan, Ika S Pinus Jumariyatno Pora, Yohana Laura Silfia Pradini, Ni Komang Virginia Prasetyaning Amukti, Danang Putri, Chintiana Nindya Rajab, Muhammad Andy Ramses Firdaus, Ramses Rina Handayani Rini Rahmawati Rosyida Awalia Safitri Rozak, Miftahul Saraswati, Damai Ira SATRIYAS ILYAS Sembiring, Rinawati Sihaloho, Serli Sitarina Widyarini Siti Athiyah Umaiyah Siti Fatimah Sultan Sudibyo Martono Sudibyo Martono Sugiyanto - Sugiyanto . Sugiyanto . Sugiyanto Sugiyanto Sugiyanto Sugiyanto Sunarti Sunarti Suryani, Arman Suwidjiyo Pramono Suwidjiyo Pramono SW, Oman Fathurohman Uddin, ABM Helal Verda Farida Wahyu Widyaningsih Wahyu Widyaningsih Wahyu Widyaningsih Wahyu Widyaningsih Wahyuningtyas, Nurma Widi Astuti, Silvia Ferry WULANDARI Yamin Yulianti, Rista Yuny Erwanto Yuny Erwanto Zahra Alya Putri