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All Journal Jurnal Veteriner Media Kedokteran Hewan Jurnal Medik Veteriner Journal of Public Health Research and Community Health Development (JPH RECODE) Jurnal Layanan Masyarakat (Journal of Public Service) Media Gizi Kesmas journal of Basic Medical Veterinary Jurnal Kedokteran Hewan Health Dynamics
Praja, Ratih Novita
Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Journal : Jurnal Kedokteran Hewan

 1 
MOLECULAR DETECTION OF GENE RESISTANT TO VARIOUS INSECTICIDES IN Aedes aegypti AT BANYUWANGI EAST JAVA USING POLYMERASE CHAIN REACTION Aditya Yudhana; Ratih Novita Praja; Maya Nurwartanti Yunita
Jurnal Kedokteran Hewan Vol 12, No 2 (2018): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (175.022 KB) | DOI: 10.21157/j.ked.hewan.v12i1.7625

Abstract

The purpose of this study was to determine the type of insecticides which Aedes aegypti mosquitoes are resistant to, so that other susceptibleinsecticides still can be used. The study was a cross sectional epidemiological study with cluster sampling in Sub-district Wongsorejo,Banyuwangi, Muncar, Tegaldlimo, Kalibaru which is considered sufficient to represent Banyuwangi Regency. Mosquito samples appropriate tothe characteristics were isolated and tested for insecticide-resistance primer namely voltage gated sodium channel (VGSC) to determine thespecific resistance expressed in mosquitoes. The result of resistance test using WHO standard method showed that Aedes aegypti mosquito fromBanyuwangi Regency were resistant to cypermethrin 0.25% and malathion 0.8%. There was a VGSC coding gene with 250 bp band detectedusing polymerase chain reaction (PCR) technique which was associated with the resistance of Aedes aegypti mosquitoes in Banyuwangi Regencyto organophosphate insecticides (malathione) and pyrethroid insecticides (cypermethrin).
CHARACTERIZATION OF VirB4 PROTEIN OF LOCAL ISOLATE Brucella abortus WITH WESTERN BLOTTING TECHNIQUE Ratih Novita Praja; Didik Handijatno; Setiawan Koesdarto; Aditya Yudhana
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.411 KB) | DOI: 10.21157/j.ked.hewan.v12i1.8091

Abstract

This research aimed to characterize VirB4 protein of local isolate Brucella abortus with Western blotting method. The result showed that there were four protein bands with molecular weights of 64.61, 59.25, 21.63, and 16.70 kDa by triggering a reaction between the whole Brucella abortus and anti-Brucella abortus serum. The results also revealed that there was only one protein band with a molecular weight of 59.25 kDa triggering a reaction between the whole Brucella abortus and anti-VirB Brucella abortus serum. Finally, it can be concluded that VirB4 protein can affect the virulence factor of Brucella abortus, successfully characterized with the appearance of one band with a molecular weight of 59.25 kDa by using Western blotting method.
MOLECULAR DETECTION OF GENE RESISTANT TO VARIOUS INSECTICIDES IN Aedes aegypti AT BANYUWANGI EAST JAVA USING POLYMERASE CHAIN REACTION Yudhana, Aditya; Praja, Ratih Novita; Yunita, Maya Nurwartanti
Jurnal Kedokteran Hewan Vol 12, No 2 (2018): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i2.7625

Abstract

The purpose of this study was to determine the type of insecticides which Aedes aegypti mosquitoes are resistant to, so that other susceptibleinsecticides still can be used. The study was a cross sectional epidemiological study with cluster sampling in Sub-district Wongsorejo,Banyuwangi, Muncar, Tegaldlimo, Kalibaru which is considered sufficient to represent Banyuwangi Regency. Mosquito samples appropriate tothe characteristics were isolated and tested for insecticide-resistance primer namely voltage gated sodium channel (VGSC) to determine thespecific resistance expressed in mosquitoes. The result of resistance test using WHO standard method showed that Aedes aegypti mosquito fromBanyuwangi Regency were resistant to cypermethrin 0.25% and malathion 0.8%. There was a VGSC coding gene with 250 bp band detectedusing polymerase chain reaction (PCR) technique which was associated with the resistance of Aedes aegypti mosquitoes in Banyuwangi Regencyto organophosphate insecticides (malathione) and pyrethroid insecticides (cypermethrin).
CHARACTERIZATION OF VirB4 PROTEIN OF LOCAL ISOLATE Brucella abortus WITH WESTERN BLOTTING TECHNIQUE Praja, Ratih Novita; Handijatno, Didik; Koesdarto, Setiawan; Yudhana, Aditya
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i1.8091

Abstract

This research aimed to characterize VirB4 protein of local isolate Brucella abortus with Western blotting method. The result showed that there were four protein bands with molecular weights of 64.61, 59.25, 21.63, and 16.70 kDa by triggering a reaction between the whole Brucella abortus and anti-Brucella abortus serum. The results also revealed that there was only one protein band with a molecular weight of 59.25 kDa triggering a reaction between the whole Brucella abortus and anti-VirB Brucella abortus serum. Finally, it can be concluded that VirB4 protein can affect the virulence factor of Brucella abortus, successfully characterized with the appearance of one band with a molecular weight of 59.25 kDa by using Western blotting method.
Co-Authors Agustono, Bodhi Al-Madinah, Wardatul Qoryah Al-Zuhroh, Tisya Yumn Aliyyah Revinda Bima Putri Amelia, Ezanti Nur Anastasya, Anastasya Andini, Zahrotul Arifin, Khoirul Arimbi Audina Putri Geraldine Aziz, Githa Nurma Baroroh, Diana Nova Boedi Setiawan Budi Utomo Chusniati, Sri Citra Kurnia Putri Diana Agustina Diansanto Prayoga Didik Handijatno Didik Handijatno Dwi, Welyanto Kurniawan Endang Suprihati Erma Safitri Estoepangestie, Agnes Theresia Soelih Fachrur Rozi Faisal Fikri Faizah, Ayu Nur Farezi, Reza Adrio Farhan, Muhammad Riesta Febriana, Melati Octavia Firmansyah, Januar Fransiska Okta Zania Fransiska, Eunike Melanda Haditanojo , Wiyanto Haditanojo, Wiyanto Hairi, Farizah Mohd Hamonangan, Jonathan Mark Harelas, Genoveva Callista Hayati, Laila Nur Herdiansyah, Akbar Dimas Hidajati, Nove Hilmin, Siti Nur Hutahaean, Ryan David Pandapotan Ichi Arum Galerani Indriyani, Dwi Putri Insani, Alivia Khairina Iwan Sahrial Hamid Izzato Millati Jayanti Dian Eka Sari, Jayanti Dian Kartikasari, Anjani Marisa Kenconojati, Hapsari Khasana, Uswatun Kusnoto Kusnoto, Kusnoto Kusuma, Agung Jati Kusumastuti, Imbi Lailiyah , Syifa'ul Lestari, Dewi Sri Mafruchati, Maslichah Mandagi, Ayik Mirayanti Masyitoh, Herlina Meles, Dewa Ketut Mufasirin Muhammad Thohawi Elziyad Purnama Muhammad, Abdul Latif Mumtazi, Fauzan Muslimah, Bintang Nor, Roslindawani Md Nurfaizah, Diza Ulya Nurwartanti, Maya Nusdianto Triakoso Poedji Hastutiek Praja, Shifa Salsabilla Prameswari, Nindya Pradnya Prameswari, Ratna Ajeng Prasetyo, Dhenatra Rifqy Prastiya, Ragil Angga Pudji Srianto Puspikawati, Septa Indra Putri, Firrial Eksa Maulidania Rahmahani, Jola Ramadhan, Alfian Dzaka Fadhil Ramadhanny, Syahrul Novenda Ratna Damayanti Rofiqi, Mohammad Miftahur Romadhona, Ma'rifatunnisa' Saksono, Bayu Saliluddin, Suhainizam Bin Muhamad Sandy, Berliana Dwi Nandita Santoso, Kuncoro Puguh Saputro, Amung Logam Sari, Devi Indah Sarudji, Suryanie Satria, Rafif Galih Sekarsari, Dara Ayu Pramesti Setiawan Koesdarto Setiawati, Puji Aris Soetojo Soetojo Solikhah, Tridiganita Intan Sri Pantja Madyawati, Sri Pantja Sukarno, Gerda Sunarso, Agus Suroiyah, Fifi Anik Susilo, Rahadian Indarto Suwarno Suwarno Tiara Wandira Hariyanto Tri Lestari Tri Wahyu Suprayogi Tyasningsih, Wiwiek Wahjuni, Retno Sri Warsito, Sunaryo Hadi Wibawati, Prima Ayu Wijaya, Achmad Arby Wilujeng, Estiary Yudhana, Aditya Yulianti, Yunika Tri Yumna, Raissa Amelia Yunita, Maya Nurwartanti Zahrudin, Elham