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Journal : Media Kedokteran Hewan

Exploration of the Antibacterial Potential from Rice Eel Skin Mucus (Monopterus albus) Against Bacteria Aeromonas hydrophila, Escherichia coli, and Staphylococcus aureus Praja, Ratih Novita; Yudhana, Aditya; Handijatno, Didik; Al-Madinah, Wardatul Qoryah; Hamonangan, Jonathan Mark; Insani, Alivia Khairina; Prameswari, Nindya Pradnya; Prasetyo, Dhenatra Rifqy; Praja, Shifa Salsabilla
Media Kedokteran Hewan Vol. 35 No. 3 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i3.2024.197-205

Abstract

The skin mucus of the rice eel (Monopterus albus) contains various antibacterial compounds and has the potential as a synthetic antibiotic. This research was conducted to explore the potential antibacterial power of the rice eel skin mucus against some pathogenic bacteria in freshwater fish. The bacteria were isolated from five samples of rice eel cultivation ponds belonging to Mr. Sabwan and then challenged with the mucus of the eel's skin through diffusion tests using paper discs. The rice eel skin mucus tested its antibacterial activity against three species of freshwater bacteria, Aeromonas hydrophila, Escherichia coli, and Staphylococcus aureus, by testing sensitivity and inhibitory zones. The inhibitory zones of each bacterium were measured using Vernier caliper which refers to the standardization of the inhibitory zone: < 4 mm no activity, 5-9 mm weak, 10- 14 mm medium, and > 15 mm strong. Test results showed that the eels' skin mucus could inhibit the growth of bacteria Aeromonas hydrophila, Escherichia coli, and Staphylococcus aureus.
Identification of Gram-Negative Bacteria in the Oral Cavity of Homalopsis buccata Praja, Ratih Novita; Yudhana, Aditya; Amelia, Ezanti Nur; Ramadhan, Alfian Dzaka Fadhil; Zahrudin, Elham; Setiawati, Puji Aris; Yumna, Raissa Amelia; Andini, Zahrotul; Nor, Roslindawani Md
Media Kedokteran Hewan Vol. 36 No. 2 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i2.2025.163-172

Abstract

The kadut belang snake (Homalopsis buccata) is a semi-aquatic snake that can be aggressive when threatened. H. buccata is often used as a pet, a source of animal protein, and raw materials for leather crafts because of its relatively large size and unique pattern. The increasing trend of keeping reptiles as pets could increase the potential for transmission of bacteria to humans. The purpose of this study was to isolate and identify Aeromonas hydrophila, Escherichia coli, Klebsiella pneumoniae, and Salmonella sp. from H. buccata. This type of research uses the accidental sampling method as the research design. The H. buccata criteria used in this study came from wild catches and were imported during the study period. Isolation was carried out to obtain separate bacterial colonies and identification of bacteria was carried out by Gram staining and biochemical reaction tests. Identification of Gram-Negative Bacteria in the Oral Cavity showed that A. hydrophila, E. coli, K. pneumoniae, and Salmonella sp. with a percentage of 33.3%, 11.1%, 55.5%, and 22.2% in the digestive tract of H. buccata snakes in the Mojokerto City area.
Identifikasi Salmonella sp. pada Susu Segar di Peternakan Sapi Perah Kecamatan Licin Kabupaten Banyuwangi Satria, Rafif Galih; Hamid, Iwan Sahrial; Wibawati, Prima Ayu; Estoepangestie, Agnes Theresia Soelih; Saputro, Amung Logam; Praja, Ratih Novita
Media Kedokteran Hewan Vol. 32 No. 3 (2021): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v32i3.2021.114-118

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui tingkat cemaran bakteri Salmonella sp. pada susu segar dari peternakan sapi perah di kecamatan licin kabupaten banyuwangi. Sampel susu segar di ambil pada april 2021 sebanyak 16 sampel dari KPSP Ijen makmur di kecamatan Licin Kabupaten Banyuwangi. Sampel diambil sebanyak 25 ml dari masing-masing peternak. Hasil yang di dapat setelah melakukan isolasi dan identifikasi menggunakan media Salmonella Shigella Agar (SSA), dan dilanjutkan uji identifikasi menggunakan TSIA dan Urea didapatkan  dari ke 16 sampel semua dinyatakn negatif bakteri Salmonella sp.. Hal ini dapat terjadi karena para peternak sapi perah di Kecamatan licin sudah mulai menyadari pentingnya kebersihan kandang dan sanitasi kandang yang baik sehingga tingkat cemaran bakteri Salmonella sp. dapat dicegah.
First case report of Salmonella spp. infection in Homalopsis buccata snake: Laporan Pertama Kasus Infeksi Salmonella spp. pada Ular Homalopsis buccata Tiara Wandira Hariyanto; Amelia, Ezanti Nur; Tyasningsih, Wiwiek; Rahmahani, Jola; Praja, Ratih Novita; Yudhana, Aditya; Nurwartanti, Maya; Fikri, Faisal
Media Kedokteran Hewan Vol. 34 No. 2 (2023): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v34i2.2023.128-134

Abstract

Reptiles are a source of transmission of pathogenic bacteria to humans. Salmonella spp. are Gram-negative bacteria that are commonly found in the digestive tract of snakes. This study was conducted on Homalopsis buccata snakes with samples obtained by oral swabs and cloacal swabs on snakes. Isolation of Salmonella spp. carried out on Tetrathionate Broth media enrichment and will be inoculated on specific media in the form of Salmonella Shigella Agar media. Identification of bacteria was carried out by Gam staining and Indole, Methyl Red, Voges Proskauer and Citrate test. This study revealed the presence of Salmonella spp. in the snake Homalopsis buccata.
Co-Authors Agustono, Bodhi Al-Madinah, Wardatul Qoryah Al-Zuhroh, Tisya Yumn Aliyyah Revinda Bima Putri Amelia, Ezanti Nur Anastasya, Anastasya Andini, Zahrotul Arifin, Khoirul Arimbi Audina Putri Geraldine Aziz, Githa Nurma Baroroh, Diana Nova Boedi Setiawan Budi Utomo Chusniati, Sri Citra Kurnia Putri Diana Agustina Diansanto Prayoga Didik Handijatno Didik Handijatno Dwi, Welyanto Kurniawan Endang Suprihati Erma Safitri Estoepangestie, Agnes Theresia Soelih Fachrur Rozi Faisal Fikri Faizah, Ayu Nur Farezi, Reza Adrio Farhan, Muhammad Riesta Febriana, Melati Octavia Firmansyah, Januar Fransiska Okta Zania Fransiska, Eunike Melanda Haditanojo , Wiyanto Haditanojo, Wiyanto Hairi, Farizah Mohd Hamonangan, Jonathan Mark Harelas, Genoveva Callista Hayati, Laila Nur Herdiansyah, Akbar Dimas Hidajati, Nove Hilmin, Siti Nur Hutahaean, Ryan David Pandapotan Ichi Arum Galerani Indriyani, Dwi Putri Insani, Alivia Khairina Iwan Sahrial Hamid Izzato Millati Jayanti Dian Eka Sari, Jayanti Dian Kartikasari, Anjani Marisa Kenconojati, Hapsari Khasana, Uswatun Kusnoto Kusnoto, Kusnoto Kusuma, Agung Jati Kusumastuti, Imbi Lailiyah , Syifa'ul Lestari, Dewi Sri Mafruchati, Maslichah Mandagi, Ayik Mirayanti Masyitoh, Herlina Meles, Dewa Ketut Mufasirin Muhammad Thohawi Elziyad Purnama Muhammad, Abdul Latif Mumtazi, Fauzan Muslimah, Bintang Nor, Roslindawani Md Nurfaizah, Diza Ulya Nurwartanti, Maya Nusdianto Triakoso Poedji Hastutiek Praja, Shifa Salsabilla Prameswari, Nindya Pradnya Prameswari, Ratna Ajeng Prasetyo, Dhenatra Rifqy Prastiya, Ragil Angga Pudji Srianto Puspikawati, Septa Indra Putri, Firrial Eksa Maulidania Rahmahani, Jola Ramadhan, Alfian Dzaka Fadhil Ramadhanny, Syahrul Novenda Ratna Damayanti Rofiqi, Mohammad Miftahur Romadhona, Ma'rifatunnisa' Saksono, Bayu Saliluddin, Suhainizam Bin Muhamad Sandy, Berliana Dwi Nandita Santoso, Kuncoro Puguh Saputro, Amung Logam Sari, Devi Indah Sarudji, Suryanie Satria, Rafif Galih Sekarsari, Dara Ayu Pramesti Setiawan Koesdarto Setiawati, Puji Aris Soetojo Soetojo Solikhah, Tridiganita Intan Sri Pantja Madyawati, Sri Pantja Sukarno, Gerda Sunarso, Agus Suroiyah, Fifi Anik Susilo, Rahadian Indarto Suwarno Suwarno Tiara Wandira Hariyanto Tri Lestari Tri Wahyu Suprayogi Tyasningsih, Wiwiek Wahjuni, Retno Sri Warsito, Sunaryo Hadi Wibawati, Prima Ayu Wijaya, Achmad Arby Wilujeng, Estiary Yudhana, Aditya Yulianti, Yunika Tri Yumna, Raissa Amelia Yunita, Maya Nurwartanti Zahrudin, Elham