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All Journal Media Kedokteran Hewan Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Jurnal Agro Veteriner
Chusniati, Sri
Department Of Veterinary Microbiology - Faculty Of Veterinary Medicine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary Other

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Journal : Ovozoa: Journal of Animal Reproduction

 1 
PENGARUH PEMBERIAN MADU TERHADAP STAGING SPERMATOGENESIS DAN SEL LEYDIG PADA MENCIT (Mus musculus) YANG DIINFEKSI Toxoplasma gondii Fitri, Paraswita Eindah; Wurlina, Wurlina; Chusniati, Sri; Suwanti, Lucia Tri; Mufasirin, Mufasirin; Plumeriastuti, Hani
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (725.345 KB) | DOI: 10.20473/ovz.v8i1.2019.10-16

Abstract

The aim of this research is to know about the staging of spermatogenesis and leydig cells in mice that injected by Toxoplasma gondii after giving honey as a preventive substance. This research used 25 mice in age 2-4 month with 20-30 weight. Mice were divided into 5 groups (P0, P1, P2, P3 dan P4). P0 as a control teratment were given aquades in peroral, P1 was a group given honey in doses I (0.08ml/0.2ml/day) during 14 days in peroral, P2 was a group that given infection by T.gondii in doses 103 in peritonial, P3 was given honey in doses I 0.08ml/0.2ml/day) then injected by T.gondii, and P4 was given honey in doses II (0.12ml/0.2ml/day) then injected by T.gondii. This research conducted in 18 days, in day 14th mice were given honey and the day 15th–18th mice injected by T.gondii doses 103. The data result was analyzed with Analysis of Variant (ANOVA) and cotinued with Duncan. Comparision statistical showed with SPSS statistic 20 for Windows. The result of analysis, P0 and P1 were significantly different (p<0.05), with the increase number of spermatogenic and leydig cells. P0 and P2 also significantly different, with the decrease number of spermatogenic and leydig cells. In P2 and P3 group were not significantly different (P>0.05), also in P4 group were not significantly different with P0 and P1. The results of this research showed that honey in doses II can maintain the number of sprematogenic and leydig cells as a preventive substance against T.gondii.
Profil bakteri non spesifik dalam lendir serviks sapi perah pada fase folikuler dan fase luteal Nur, Maysarah Oktavia; Mulyati, Sri; Sardjito, Trilas; Chusniati, Sri; Tyasningsih, Wiwiek; Mafruchati, Maslichah
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (830.154 KB) | DOI: 10.20473/ovz.v9i1.2020.17-22

Abstract

This research was conducted to identify non-specific bacteria found in the cervical mucus of dairy cattle in the follicular and luteal phase. Twenty cervical mucus samples were collected from ten cows during their follicular phase and ten cows during their luteal phase. Sampling was carried out with the help of an artificial insemination gun and a plastic sheath, which were protected by a sheath cover. After insertion into cervix, the tip of the plastic sheath was cut and put into a tube prefilled with phosphate buffered saline. All samples were cultured in Blood Agar media and isolated in Eosin Methylene Blue Agar, Mac Conkey Agar, and Manitol Salt Agar. Gram staining was applied to distinguish gram-positive and gram-negative bacteria; and followed with the catalase, spore, motility, and biochemical tests to further identify bacteria. The bacteria identified were of the genus Enterobacter, Staphylococcus, Corynebacterium, Streptococcus, Escherichia, and Pasteurella. It could be concluded that non-specific bacteria of the same genus were identified in cervical mucus of dairy cows in their follicular and luteal phase, in a various of percentages.
Comparison of different poultry egg yolks-citrate extender with green tea (Camellia sinensis) extract addition on Sapudi ram spermatozoa quality in chilled temperature storage Shinta Putri Quraini; Suherni Susilowati; Tjuk Imam Restiadi; Sri Chusniati
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 3 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i3.2022.93-97

Abstract

Preservation of ram semen has an impact on the exposure of artificial conditions to the spermatozoa which was followed by overproduction of reactive oxygen species (ROS) and resulted in functional damages of spermatozoa. This study aimed to determine the effect of the combination of chicken, quail, and duck egg yolks with green tea extract (GTE) on the quality of Sapudi ram spermatozoa at 5°C storage. Ejaculates of Sapudi ram with more than 70% spermatozoa motility were divided into four groups. Semen were extended 1 : 10 in citrated-chicken egg yolk extender (control group, T0), and citrated yolk of chicken (T1), quail (T2), and duck (T3) egg added with 0.05 mg GTE per 100 mL of extender. Extended semen was kept in a tube, which was put in a beaker containing distilled water and stored in a refrigerator (5°C). Spermatozoa motility, viability, and plasma membrane integrity (PMI) were evaluated daily for five days. The results showed that GTE in duck egg yolk citrate extender could maintain the highest spermatozoa motility, viability, and PMI for five days (p <0.05). It could be concluded that the duck egg yolk citrate extender with the addition of GTE (Camellia sinensis) was beneficial for maintaining the semen of Sapudi ram spermatozoa at chilled temperatures. Further study is needed to use the extender for freezing Sapudi ram semen.
Co-Authors Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Arimbi Benjamin Christoffel Tehupuring, Benjamin Christoffel Dadik Rahardjo, Dadik Ernawati, Rahaju Faiqoh, Berliana Elok Fitri, Paraswita Eindah Gandul Atik Yuliani Hani Plumeriastuti Harijani, Nenny Hayati, Laila Nur Hidanah, Sri Hussain, Muhammad Asif Irawan, Adinda Riezki Iwan Sahrial Hamid Jannah, Sukmawati Lailatul Khairullah, Aswin Rafif Kuncorojakti, Suryo Kusnoto Kusnoto Legowo, Djoko Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Mafruchati, Maslichah Mirni Lamid Mirni Lamid Mochamad Lazuardi Moh. Anam Al Arif Moh. Sukmanadi Mohammad Sukmanadi Mufasirin Muhammad Agil Gumilang Munawer Pradana Mustofa Helmi Effendi Nayan, Nazri Nur, Maysarah Oktavia Nurliyani Nurliyani Nurrahmad, Nanda Rino Pradika, Adhitya Yhoga Praja, Ratih Novita Pribadi, Adinda Dilla Raharjo, Hartanto Mulyo Rahmahani, Jola Rahmi Sugihartuti Rahmi Sugihartuti, Rahmi Ratna Damayanti Rehman, Saifur Rendragraha, Briantono Willy Ria Nikmatul Jannah Rondius Solfaine Sabdoningrum, Emy Koestanti Setiawati Sigit Shinta Putri Quraini Soeharsono Soeharsono Sri Hidanah Sri Mulyati Sri Mumpuni Sosiawati Sri Pantja Madyawati, Sri Pantja Subagyo, Safira Fauziyyah Suherni Susilowati Sukmawati Lailatul Jannah Suwarno Suwarno Tita Damayanti Lestari Tjuk Imam Restiadi Trilas Sardjito Tyaningsih, Wiwiek Tyasningsih, Wiwiek Wahyuni, Retno Sri Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widya Paramita Lokapirnasari Wiwiek Tyasningsih Wurlina, W Yudhana, Aditya Yulianna Puspitasari Yunita, Maya Nurwartanti