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All Journal Media Kedokteran Hewan Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Jurnal Agro Veteriner
Chusniati, Sri
Department Of Veterinary Microbiology - Faculty Of Veterinary Medicine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary Other

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Effect of Vaccination Outer Membrane Protein 52 kDa on Changes in Erythrocyte Index of Tilapia (Oreochromis niloticus) Infected by Aeromonas hydrophila Nurrahmad, Nanda Rino; Yuliani, M. Gandul Atik; Ernawati, Rahaju; Chusniati, Sri; Herupradoto, Eduardus Bimo Aksono; Wahyuni, Retno Sri
Journal of Basic Medical Veterinary Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i1.28592

Abstract

The purpose of this study was to determine the effect after being vaccinated by OMP 52 kDa Aeromonas hydrophila for 1 week and then infected with Aeromonas hydrophila 10 CFU/mL for 4 days on changes in erythrocyte index. Tilapia (Oreochromis niloticus) used in this study was 10-12 cm long. There were 20 tilapia (Oreochromis niloticus) which were divided into 4 groups, consisting of 2 control groups and 2 treatment groups which were given various types of vaccine formulations. Group P0 (-) (unvaccinated and infected), Group P0 (+) (unvaccinated and infected), group P1 (vaccinated with the whole cell protein "HydroVac®" and infected), and P2 (vaccinated with Outer Membrane Protein 52 kDa and infected) by intramuscular injection. Post-treatment blood samples were collected on day 5 post-infection, collected through a caudal punctie and then analyzed using a hematology analyzer. Post-treatment outcomes led to statistically significant changes (p < 0.05). Therefore, the vaccine caused a significant change in the erythrocyte index.
The Efficacy of Putrimalu Root Extract (Mimosa pudica) Against Mortality and Histopathological of Rat Muscles Injected Naja sputatrix Venom Rendragraha, Briantono Willy; Legowo, Djoko; Kuncorojakti, Suryo; Chusniati, Sri; Arimbi, Arimbi
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.36486

Abstract

The purpose of this experiment was to know the effect of Mimosa pudica root extract (normal water extraction) on mortality and muscle histopatological in experimental animal (Rattus norvegicus) which injected with LD50 of Naja sputatrix venom. Fresh Mimosa pudica root dried without sunshine and ground into coarse powder with steamroller (mash 3mm). Plant extract were prepared by stirring 4 g of the powder into 200 ml of water for 3 hours at room temperature and filtered with muslin cloth before freezdried. 30 rat divided into five groups, P0(C+), P1(C-) P2, P3, and P4. P0 (positive control group) injected by saline intra muscular and given aquadest peroral after five minutes, P1 (negative control) injected with Naja sputatrix venom and given aquadest peroral after five minutes. P2, P3, and P4 injected by Naja sputatrix venom and given Mimosa pudica extract with 250mg/KgBW, 500mg/KgBW, and 1000mg/KgBW doses. All of the experimental animal observed about six hours and than euthanized, musculus gluteus maximus (location of injection) was taken for histopatological examination. In this experiment observed that the normal water extraction of mimosa pudica can't reduce mortality of experimental animals but it can decrease muscle damage in 1000mg/KgBW dose.
Isolation and Identification of Bacterial Biosurfactant Activity from Mangrove Sediments Sugihartuti, Rahmi; Pradana, Munawer; Maslachah, Lilik; Chusniati, Sri; Tyaningsih, Wiwiek; Rahardjo, Dadik; Soeharsono, Soeharsono
Journal of Basic Medical Veterinary Vol. 13 No. 1 (2024): Journal of Basic Medical Veterinary, June 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v13i1.58266

Abstract

Background: Ujung Pangkah mangroves are reported to have been contaminated with heavy metals. Such heavy metals can induce microorganisms to produce biosurfactants. Biosurfactants with amphiphilic characteristics can lower surface tension. Biosurfactants can be used as antibacterial, antifungal, and antiviral for biomedical purposes. Purpose: This study aimed to identify and test the activity of biosurfactant isolates of bacteria from Ujung Pangkah Mangrove sediments, Gresik Regency. Methods: Biosurfactant activity test methods include emulsification index, oil spreading, drop collapse, and parafilm test. Results: The results of the identification of bacterial isolates in this study obtained the genus Bacillus sp. because bacterial isolates show rod shape, Gram-positive, aerobic, and have ellipse-shaped endospores on the subterminal. The results of the biosurfactant activity test with the Emulsification index method showed an average result of 54.39% and the results of the biosurfactant activity test with the oil spreading method showed that there was a clear zone. The average result of the clear zone obtained is 54.83 mm. The average result in the parafilm test was 8.02 mm and the drop collapse test showed positive results characterized by falling and spreading of bacterial isolate fermentation broth supernatants.
Probiotics Addition Potential to Increase Body Weight, Feed Consumption and Feed Conversion on Pre-Layer Laying Hens Sukmawati Lailatul Jannah; Mirni Lamid; Mohammad Sukmanadi; Mohammad Anam Al Arif; Sri Hidanah; Sri Chusniati
Jurnal Agro Veteriner Vol. 7 No. 1 (2023): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v7i1.51392

Abstract

Probiotics are all forms of microbial cell preparations or components of microbial cells that have a beneficial effect on the health and life of the host. Some feed additives such as the Antibiotic Growth Promoter (AGP) hormone have been banned. Therefore, nowadays, probiotics are often used as additives to replace antibiotics. The probiotics that are widely used are lactic acid bacteria because they can increase the efficiency of digestion and absorption of nutrients. The purpose of this study was to determine the effect of giving probiotics a combination of Bacillus coagulans and Bacillus subtilis with doses of 2 ml, 4 ml, and 6 ml/head/day orally on weight gain, feed consumption, and decreased feed conversion of laying hens. This study was conducted for 2 weeks using pre layer chickens aged 21 weeks to 23 weeks old as many as 24 chickens, randomized into 4 treatments with 6 replications with P0 as control, P1 = 2 ml, P2 = 4 ml, and P3 = 6 ml. The probiotic used is a combination of probiotics Bacillus coagulans and Bacillus subtilis with a concentration of 1x107 CFU/ml in liquid.
Addition of Bacillus subtilis BN Strain Probiotic on Chicken Eggs Quality Muhammad Agil Gumilang; Widya Paramita Lokapirnasari; Mochamad Lazuardi; Mohammad Anam Al Arif; Sunaryo Hadi Warsito; Sri Chusniati
Jurnal Agro Veteriner Vol. 7 No. 1 (2023): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v7i1.51498

Abstract

The addition of probiotics is one effort to increase the chicken's appetite. There are many probiotics that can be mixed into feed to help improve the digestive and reproductive systems, including Bacillus subtilis, which has the function of increasing egg quality, including increasing shell thickness, increasing egg volume, increasing egg yolk and white index. This reseacrh aims to find out the effect of probiotic administration of Bacillus subtilis BN Strain on shell thickness, white index and chicken yolk index. This research used 38-week-old layer laying hens as many as 24 heads, randomized into four treatments with eight replays. Based on the analysis of statistical data, the Analysis Of Variance (ANOVA) test showed no noticeable difference (p>0.05) to the thickness of the shell, white index or egg yolk index. The results showed that the addition of probiotic Bacillus subtilis BN Strain as many as 0.005 grams/kg of feed, 0.01 grams/kg of feed and 0.02 kg/kg of feed had no noticeable effect on the increase in egg shell thickness, egg white index and egg yolk index.
PENGARUH PEMBERIAN MADU TERHADAP STAGING SPERMATOGENESIS DAN SEL LEYDIG PADA MENCIT (Mus musculus) YANG DIINFEKSI Toxoplasma gondii Fitri, Paraswita Eindah; Wurlina, Wurlina; Chusniati, Sri; Suwanti, Lucia Tri; Mufasirin, Mufasirin; Plumeriastuti, Hani
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (725.345 KB) | DOI: 10.20473/ovz.v8i1.2019.10-16

Abstract

The aim of this research is to know about the staging of spermatogenesis and leydig cells in mice that injected by Toxoplasma gondii after giving honey as a preventive substance. This research used 25 mice in age 2-4 month with 20-30 weight. Mice were divided into 5 groups (P0, P1, P2, P3 dan P4). P0 as a control teratment were given aquades in peroral, P1 was a group given honey in doses I (0.08ml/0.2ml/day) during 14 days in peroral, P2 was a group that given infection by T.gondii in doses 103 in peritonial, P3 was given honey in doses I 0.08ml/0.2ml/day) then injected by T.gondii, and P4 was given honey in doses II (0.12ml/0.2ml/day) then injected by T.gondii. This research conducted in 18 days, in day 14th mice were given honey and the day 15th–18th mice injected by T.gondii doses 103. The data result was analyzed with Analysis of Variant (ANOVA) and cotinued with Duncan. Comparision statistical showed with SPSS statistic 20 for Windows. The result of analysis, P0 and P1 were significantly different (p<0.05), with the increase number of spermatogenic and leydig cells. P0 and P2 also significantly different, with the decrease number of spermatogenic and leydig cells. In P2 and P3 group were not significantly different (P>0.05), also in P4 group were not significantly different with P0 and P1. The results of this research showed that honey in doses II can maintain the number of sprematogenic and leydig cells as a preventive substance against T.gondii.
Profil bakteri non spesifik dalam lendir serviks sapi perah pada fase folikuler dan fase luteal Nur, Maysarah Oktavia; Mulyati, Sri; Sardjito, Trilas; Chusniati, Sri; Tyasningsih, Wiwiek; Mafruchati, Maslichah
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (830.154 KB) | DOI: 10.20473/ovz.v9i1.2020.17-22

Abstract

This research was conducted to identify non-specific bacteria found in the cervical mucus of dairy cattle in the follicular and luteal phase. Twenty cervical mucus samples were collected from ten cows during their follicular phase and ten cows during their luteal phase. Sampling was carried out with the help of an artificial insemination gun and a plastic sheath, which were protected by a sheath cover. After insertion into cervix, the tip of the plastic sheath was cut and put into a tube prefilled with phosphate buffered saline. All samples were cultured in Blood Agar media and isolated in Eosin Methylene Blue Agar, Mac Conkey Agar, and Manitol Salt Agar. Gram staining was applied to distinguish gram-positive and gram-negative bacteria; and followed with the catalase, spore, motility, and biochemical tests to further identify bacteria. The bacteria identified were of the genus Enterobacter, Staphylococcus, Corynebacterium, Streptococcus, Escherichia, and Pasteurella. It could be concluded that non-specific bacteria of the same genus were identified in cervical mucus of dairy cows in their follicular and luteal phase, in a various of percentages.
Comparison of different poultry egg yolks-citrate extender with green tea (Camellia sinensis) extract addition on Sapudi ram spermatozoa quality in chilled temperature storage Shinta Putri Quraini; Suherni Susilowati; Tjuk Imam Restiadi; Sri Chusniati
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 3 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i3.2022.93-97

Abstract

Preservation of ram semen has an impact on the exposure of artificial conditions to the spermatozoa which was followed by overproduction of reactive oxygen species (ROS) and resulted in functional damages of spermatozoa. This study aimed to determine the effect of the combination of chicken, quail, and duck egg yolks with green tea extract (GTE) on the quality of Sapudi ram spermatozoa at 5°C storage. Ejaculates of Sapudi ram with more than 70% spermatozoa motility were divided into four groups. Semen were extended 1 : 10 in citrated-chicken egg yolk extender (control group, T0), and citrated yolk of chicken (T1), quail (T2), and duck (T3) egg added with 0.05 mg GTE per 100 mL of extender. Extended semen was kept in a tube, which was put in a beaker containing distilled water and stored in a refrigerator (5°C). Spermatozoa motility, viability, and plasma membrane integrity (PMI) were evaluated daily for five days. The results showed that GTE in duck egg yolk citrate extender could maintain the highest spermatozoa motility, viability, and PMI for five days (p <0.05). It could be concluded that the duck egg yolk citrate extender with the addition of GTE (Camellia sinensis) was beneficial for maintaining the semen of Sapudi ram spermatozoa at chilled temperatures. Further study is needed to use the extender for freezing Sapudi ram semen.
Co-Authors Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Arimbi Benjamin Christoffel Tehupuring, Benjamin Christoffel Dadik Rahardjo, Dadik Ernawati, Rahaju Faiqoh, Berliana Elok Fitri, Paraswita Eindah Gandul Atik Yuliani Hani Plumeriastuti Harijani, Nenny Hayati, Laila Nur Hidanah, Sri Hussain, Muhammad Asif Irawan, Adinda Riezki Iwan Sahrial Hamid Jannah, Sukmawati Lailatul Khairullah, Aswin Rafif Kuncorojakti, Suryo Kusnoto Kusnoto Legowo, Djoko Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Mafruchati, Maslichah Mirni Lamid Mirni Lamid Mochamad Lazuardi Moh. Anam Al Arif Moh. Sukmanadi Mohammad Sukmanadi Mufasirin Muhammad Agil Gumilang Munawer Pradana Mustofa Helmi Effendi Nayan, Nazri Nur, Maysarah Oktavia Nurliyani Nurliyani Nurrahmad, Nanda Rino Pradika, Adhitya Yhoga Praja, Ratih Novita Pribadi, Adinda Dilla Raharjo, Hartanto Mulyo Rahmahani, Jola Rahmi Sugihartuti Rahmi Sugihartuti, Rahmi Ratna Damayanti Rehman, Saifur Rendragraha, Briantono Willy Ria Nikmatul Jannah Rondius Solfaine Sabdoningrum, Emy Koestanti Setiawati Sigit Shinta Putri Quraini Soeharsono Soeharsono Sri Hidanah Sri Mulyati Sri Mumpuni Sosiawati Sri Pantja Madyawati, Sri Pantja Subagyo, Safira Fauziyyah Suherni Susilowati Sukmawati Lailatul Jannah Suwarno Suwarno Tita Damayanti Lestari Tjuk Imam Restiadi Trilas Sardjito Tyaningsih, Wiwiek Tyasningsih, Wiwiek Wahyuni, Retno Sri Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widya Paramita Lokapirnasari Wiwiek Tyasningsih Wurlina, W Yudhana, Aditya Yulianna Puspitasari Yunita, Maya Nurwartanti