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All Journal HAYATI Journal of Biosciences Jurnal Gizi dan Pangan ACTA VETERINARIA INDONESIANA Jurnal Pengolahan Hasil Perikanan Indonesia Hemera Zoa Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner BIOTROPIA - The Southeast Asian Journal of Tropical Biology Medical Journal of Indonesia Current Biochemistry Jurnal Ilmu Ternak Veteriner Warta IHP (Warta Industri Hasil Pertanian) Historika Jurnal Medik Veteriner ARSHI Veterinary Letters FITOFARMAKA: Jurnal Ilmiah Farmasi Majalah Patologi Indonesia Community Development Journal: Jurnal Pengabdian Masyarakat KIDDO: Jurnal pendidikan Islam Anak Usia Dini Indonesian Journal of Applied Research (IJAR) International Journal of Multidisciplinary: Applied Business and Education Research Journal of Education Research Jurnal Pusat Inovasi Masyarakat Jurnal Kedokteran Hewan Journal of Research and Publication Innovation VALUTA Jurnal Administrasi Bisnis, Manajemen dan Ekonomi Policy Brief Pertanian, Kelautan, dan Biosains Tropika Health Gate Jurnal Agripet Seminar Nasional Riset dan Teknologi (SEMNAS RISTEK) Neraca: Jurnal Ekonomi, Manajemen dan Akuntansi
Agus Setiyono
Departemen Klinik, Reproduksi Dan Patologi Fakultas Kedokteran Hewan, Institut Pertanian Bogor. Jl. Agatis Kampus IPB Dramaga 16680
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Journal : Jurnal Veteriner

 1 
Deteksi Coxiella burnetii pada Sapi Kurban Idhul Adha di Cimanggu, Kabupaten Bogor Tahun 2015-2016 (DETECTION OF COXIELLA BURNETII IN IED AL-ADHA SACRIFICIAL COW IN CIMANGGU, BOGOR REGENCY AT 2015-2016) Handayu Untari; Agus Setiyono; Ekowati Handharyani; Masdiana C. Padaga
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Coxiella burnetii is an intracellular obligate bacterium that causes Q fever disease. Q fever is one of the asymptomatic zoonosis and has a potential as biological weapon. This disease infects much type of animals and has been distributed globally, including in Indonesia. Previous research concluded that West Java is an endemic area of this disease. Bogor in West Java has a potential spread of Q fever disease due to high population of cattle and goat in this region.This study aim was to detect C. burnetii in spleen, lung, kidney, liver and heart of cows. Samples of were taken from a total of 29 sacrificial cows during Eid al-Adha in 2015 and 2016 in the Cimanggu region, Bogor Regency. The examination method used was immunohistochemistry with primary polyclonal antibody Rabbit anti-C. burnetii and HaematoxylineEosine staining. The results of the immunohistochemistry examination showed positive immunoreaction (specific brown color in the cytoplasm of cells) against C. burnetii antibodies respectively in 3 out of 10 samples in 2015 (30%) and 4 out of 19 samples in 2016 (21%). Out of 29 samples, positive immunoreaction were found in lung organ (13,7%) and spleen (10,3%) with variation in changes in histopathological features which include congestion, inflammatory cell infiltration, mucus accumulation, and fibrosis in the lung.
Deteksi Coxiella burnetii Penyebab Q fever pada Sapi, Domba dan Kambing di Bogor dan Bali (DETECTION OF COXELLA BURNETII, THE CAUSAL AGENT OF Q FEVER Hapsari Mahatmi; Agus Setiyono; Retno Damayanti Soejoedono; Fachriyan Hasmi Pasaribu
Jurnal Veteriner Vol 8 No 4 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study to detect Coxiella burnetii, an intracellular bacterium causing Q fever in human and livestock animals, was carried out in several ruminants in Bogor and Bali. The methods used for the detection was Nested-Polymerase Chain Reaction (Nested-PCR). Two pairs of primers, the first (OMP1 and OMP2) and the second (OMP3 and OMP4) were used to detect the genomic sequences and the conserved specific sequences of Coxiella burnetii, respectively. Organ samples such as liver and lung from 410 livestock ruminants, consisting of cattle (245 samples), sheep (105 samples) and goats (60 samples) were collected from several slaughter houses in Bogor and Bali. As many as 15 (6.12%) out of 245 cattle, 6 (5.71%) out of 105 sheep and none from goat were infected by Coxiella burnetii. Interestingly, 3 out of 15 infected cattle were Bali cattle. The results clearly indicate that Q fever is likely to be widespread among ruminant animals in Indonesia.
Profil Kinetik dan Efektivitas Enrofloksasin yang Dikombinasikan dengan BioATP dalam Mengatasi Coxiella burnetii (KINETIC PROFILE AND EFFECTIVITY OF ENROFLOXACINE WITH BIO ADENOSIN TRIPHOSPHATE SUPPLEMENTATION AGAINST COXIELLA BURNETII) Andriyanto .; Agus Setiyono; Min Rahminiwati; Neni Nuryani; Unang Patriana
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Coxiella burnetii belongs to rikettsia group living obligate intracellularly and as the agent of zoonosisQ fever. Enrofloxacine is an antibiotic in quinolon group used to treat infection of C. burnetii in chicken,goat, calve, pig, dog, cat,  and horse. From ruminant practical experience, enrofloxacine if combined withBioATP  can enhance the enrofloxacine activity. Research for the effecivity of enrofloxacine and BioATP totreat C. burnetii has never been carried out. The research was conducted to explore effect of enrofloxacinewith supplementation BioATP against C. burnetii. Enrofloxacine pharmacokinetic study was carried outby using simental beef as an experimental animals. The effectivity of BioATP supplementation onenrofloxacine activity to treat C. burnetii was tested by using Vero cell tissue culture. The results showedthat combination of enrofloxacine and BioATP increased kinetic profile of enrofloxacine in term of onset,duration, pharmacology intensity, and bioavailaibility. Enrofloxacine had activity to treat C. burnetii withvalue of minimal inhibitory concentration (MIC) at 1-2 ppm and value of minimal bactericidal concentrationat 4 ppm. Supplementation of BioATP improved the effectivity of enrofloxacine in treating C. burnetii.
Sensitivitas dan Spesifisitas Nested Polymerase Chain Reaction untuk Mendeteksi DNA Coxiella burnetii (SENSITIVITY AND SPECIFICITY OF NESTED POLYMERASE CHAIN REACTION FOR DETECTION OF COXIELLA BURNETII DNA) Trioso Purnawarman; I Wayan Teguh Wibawan; Fachriyan Hasmi Pasaribu; Agus Setiyono; Muharam Saepulloh
Jurnal Veteriner Vol 13 No 1 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Sensitivity and specificity of nested polymerase chain reaction (nested PCR) to detect Coxiella burnetii(C. burnetii) DNA were studied. The primer system which consists of external primers (OMP1 and OMP2)and internal primers (OMP3 and OMP4), was designed from the nucleotide sequence of the com I geneencoding for 27 kDa outer membrane protein and used to specifically amplify a 501 bp and 438 bp fragment.This nested PCR assay was 50 fold more sensitive than that of using PCR external primer only. TheNested PCR has a detection limit as low as 300 pg/?l. Specificity studies showed that nested PCR onlydetected C. burnetii DNA and did not happened Brucella abortus, Escherichia coli, Pseudomonas aeruginosaand Campylobacter Jejuni DNA. Nested PCR has high senstively and specificaly diagnostic method of C.burnetii as agent of Q fever disease.
Co-Authors Aditya Dharmawan, Aditya Aditya Wahyu Nugraha Afifah, Amalia Aisjah Girinda Akhmad Endang Zainal Hasan Akterono D. Budiyati Allaily Allaily Alvin Jefry Amrozi Andre, Daniel Latief Andriyanto . Anggraini , Ninik Anita Esfandiari Arif Yahya Arifianto, Dinar Ario Damar Aulia Dina Kristina Bayu Sandi Chintia Ayu Puspita Darsono Darsono Desdiani Diah Nugrahani Pristihadi Dian Pratiwi DIMAS ANDRIANTO Djumali Mangunwidjaja Dwi Astuti E. Mulyati Effend Effend, E. Mulyati Eko Prasetyo Nugroho Eko Suryanto Ekowati Handharyani Elok Puspita Rini Elpita Tarigan Eny Nur Aisyah Evy Damayanthi Fachriyan Hasmi Pasaribu Feri Kusnandar Gani, Ainal Gatut Ashadi Handayu Untari Handayu Untari Hanif Alamudin Manshur Hapsari Mahatmi Hardika Hardika, Hardika Hasim Hasim Hasim Hasim Hasim Hasim Hasim Hasim, . Hendry, Altaff Husnawati, . I Ketut Suada I Komang Gede Wiryawan I wayan Teguh Wibawan Idqan Fahmi Iftitah, Selfi Lailiyatul Ike Agustiani Imron Arifin IPB, DGB Iris Rengganis Ismah Atika Salmah Japanis Permatasari, Retno Jusman, Sri Widia Azraki Kustiariyah Tarman Lala M Kolopaking Liesbetini Haditjaroko Lily Natalia M Agus Setiadi M. Aman Yaman M. Misbah, M. Ma'mun Sarma Maharani Maharani, Maharani Maningtyas, Rosydamayani Twinsari Maningtyas, Rosyidamyani Twinsari Masdiana C. Padaga Mega Safithri Miko Afrian Mochamad Alfinanda Santriagung Mohammad Irham Bagus Santoso Mohammad Sadikin Muhammad Daud Muhammad Ihsan Muhammad Ridla Muhammad Zuhdi Badruzzaman Muhammad Zulkifli Muharam Saepulloh Mutholaah Nahrowi Nahrowi Nahrowi Nathasia, Novena Neni Nuryani Nina Herlina Nurjati Chairani Siregar Nurliani Bermawie Nurliani Bermawie Nurliani Bermawie Nurrahman, Arif Oktariza, Wawan Olive Wisepti Pratama Ono Suparno Permana, Nadine Hanifa Pramono Prastyo, Danang Pribadi, Yohanes Kusuma Purnamiharja, Tsulatsy Puspitasari Purwiyatno Hariyadi Putri C. Eyanoer Rachmat Nabib Rahman, Muhammad Luthfi Rahminiwati, Min Rayendra, Raendi Restu Librani Retno Damayanti Soejoedono Risa Tiuria Rudramurti, Win Satya Samsuridjal Djauzi Sandi, Bayu Sangko Sayuti Nasution Sangko Sayuti Nasution, Sangko Santi Noviasari Sekar Winahyu Ariadini Setiadi, Hirawan Shila Rahmafia Putri SILMI MARIYA Siti Zulaikhah Slamet Budijanto Sri Purwaningsih, Sri Sri Wahyuni Salam Sudradjat Sumiati Suradi Suria Darma Tarigan Syahruddin Said Syifa Khaerani Titi Candra Sunarti Tomas Iriyanto Tri Isyani Tungga Dewi Trioso Purnawarman Ulfah Juniarti Siregar Unang Patriana Upik Kesumawati Hadi Utami, Dian Maulia Utamimah, Sri Vetnizah Juniantito Winahyu Ariadini, Sekar Wiranda G Piliang Wiwin Winarsih Wiyono, W. WoroWiranti, Rahayu Yan, Teo Qin Yeni Kartika Sari, Yeni Kartika