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All Journal Jurnal Akuakultur Indonesia Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia BERITA BIOLOGI Jurnal Akuatika Indonesia Omni-Akuatika Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Sains Natural: Journal of Biology and Chemistry Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Tolis Ilmiah: Jurnal Penelitian Clarias : Jurnal Perikanan Air Tawar Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine Jurnal Perikanan
Dinar Tri Soelistyowati
Department Of Aquaculture, Faculty Of Fisheries And Marine Sciences, IPB University Bogor, West Java, 16680. Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Social Sciences

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EVALUATION OF RESISTANCE AND GENE EXPRESSION OF BARRAMUNDI, Lates calcarifer POST-INFECTION OF NERVOUS NECROSIS VIRUS Fitriyah Husnul Khotimah; Alimuddin Alimuddin; Dinar Tri Soelistyowati; Sri Nuryati; Ketut Sugama; Ahmad Muzaki; Indah Mastuti; Sari Budi Moria Sembiring; Ketut Mahardika; Harton Arfah; Haryanti Haryanti
Indonesian Aquaculture Journal Vol 17, No 2 (2022): (December, 2022)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.17.2.2022.97-106

Abstract

The most common problem in barramundi Lates calcarifer seedling production is the high mortality (> 90%) caused by nervous necrosis virus (NNV) infection. This research aims to evaluate the resistance and gene expression of barramundi challenged by NNV. Two populations were used in this study, i.e., Australian, and Situbondo-originated barramundi populations. The immune-related gene expression levels in the liver, head of kidney, and spleen were observed at 48 and 96 hours after post-infection (hpi). Barramundi’s survival and blood parameters were evaluated post-NNV infection. The results showed that the highest survival was revealed in Situbondo’s barramundi (42.0±4.47%) compared to Australian barramundi (20.0±7.07%) and no mortality was observed in the control without NNV infection. The higher survival rate in barramundi from Situbondo was in line with the blood profile. The number of red blood cell from Situbondo barramundi post-NNV infection (ST) at 96 hpi was higher (P<0.05) than Australian barramundi post-NNV infection (AT). The number of white blood cell of ST at 48 hpi was higher (P<0.05) than AT, but started to decrease at 96 hpi in ST barramundi. The total white blood cell in AT barramundi increased from 48 to 192 hpi. TNFα and IL1-β gene expression levels were significantly higher in the liver, head kidney, and spleen of Situbondo compared to Australian barramundi at 48 hpi, while MHCIIα gene expression in Situbondo’s was significantly higher compared to Australian barramundi at 96 hpi. These results indicate the important roles of all the genes in the barramundi’s immune responses against viral infection. Based on the results of the research, Situbondo’s barramundi has the potential to be used as a candidate for generating broodstock of disease-resistant strain.
Molecular Assessment of Kappaphycus alvarezii Cultivated in Tarakan based on cox2-3 Spacer Gloria Ika Satriani; Dinar Tri Soelistyowati; Alimuddin Alimuddin; Harton Arfah; Irzal Effendi
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 18, No 1 (2023): May 2023
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.736

Abstract

The seaweed Kappaphycus alvarezii is a leading aquaculture commodity possessing high economic value that has been used as a raw material for various natural products, food, and pharmaceutical industries. The main challenge regarding its production, especially in Tarakan, is the supply of superior seeds. Therefore, this study aims to obtain phylogenetic information and the cultivation performance of the selected seeds by identifying genetic sources based on molecular markers. DNA sequencing was analyzed using the molecular marker cyclooxygenase 2-3 intergenic spacer (cox2-3 spacer) on the 16 cultivars collection from around Indonesia. Four haplotypes representing the K. alvarezii variant to be cultivated in Tarakan City were produced based on the phylogenetic clustering and further molecular analysis using a cox2-3 spacer mitochondrial DNA marker. These include the Kupang and Natuna varieties, also referred to as Lampung seedlings, which is a tissue culture plantlets used for development at SEAMEO-BIOTROP Bogor and propagated by the Lampung Marine Aquaculture Center (BBPBL), Tarakan. These varieties have genetic distances (pairwise comparisons based on the mean Kimura 2-parameter model) ranging from 0.000-0.243 units. The cultivation was carried out for 30 days in the waters of Tanjung Batu (Tj. Batu) Mamburungan, Tarakan, North Kalimantan. The result showed that the Lampung cultivar (Natuna) has a superior daily growth rate (DGR) and carrageenan yield compared to the local cultivars (Mamburungan Tanjung Batu (Takalar), Tarakan, and Kupang). Furthermore, the genetic analysis with cox2-3 spacer markers could be an effective seed selection tool for tracing genetic information in developing superior seedlings in aquaculture activities.
Selection and cultivation of seaweed Kappaphycus alvarezii using a long line system (outdoor) Gloria I. Satriani; Dinar Tri Soelistyowati; Alimuddin Alimuddin; Harton Arfah; Irzal Effendi
Depik Vol 12, No 3 (2023): DECEMBER 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.3.30211

Abstract

In outdoor cultivation at Amal Beach, three seeding mass selections were reared for 30 days for each cycle. Each variety had a different DGR %/day (Daily Growth Rate %/day) value at the end of the growing season on G1-G2-G3 ranging from 3.62-5.90 %/day, which were still categorized as good, and G3 var.Lampung had the highest DGR value. Carrageenan yield values in this study, G1 to G3, ranged from 23.16% to 45.87%, where the G3 G2 G1 treatment. The best thallus samples (20% were selected for each generation) resulted in carrageenan yield sample population in each variety, where var.Lampung var.Tarakan var.Kupang. The biplot analysis shows that the DGR variable vector coincides with the IAA, Phosphate, Gibberellin, and Ammonia vectors. Ammonia stepwise regression analysis (sigα) affects DGR with an Ammonia coefficient value of 4.89; meaning that for each increase in the Ammonia value by one unit, the estimated mean value of DGR will increase by 4.89. Thus, the application of the mass based on the results of this study was able to increase the DGR value Kappaphycus alvarezii, which in turn produce the highest yield of carrageenan with K. alvarezii var.Lampung G3 was the best seed candidate to be developed at the seaweed cultivation site in Tarakan City.Keywords:AlgaePhytohormonesCarrageenanNitrogenViscosity
Selection and cultivation of seaweed Kappaphycus alvarezii using a long line system (outdoor) Gloria I. Satriani; Dinar Tri Soelistyowati; Alimuddin Alimuddin; Harton Arfah; Irzal Effendi
Depik Vol 12, No 3 (2023): DECEMBER 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.3.30211

Abstract

In outdoor cultivation at Amal Beach, three seeding mass selections were reared for 30 days for each cycle. Each variety had a different DGR %/day (Daily Growth Rate %/day) value at the end of the growing season on G1-G2-G3 ranging from 3.62-5.90 %/day, which were still categorized as good, and G3 var.Lampung had the highest DGR value. Carrageenan yield values in this study, G1 to G3, ranged from 23.16% to 45.87%, where the G3 G2 G1 treatment. The best thallus samples (20% were selected for each generation) resulted in carrageenan yield sample population in each variety, where var.Lampung var.Tarakan var.Kupang. The biplot analysis shows that the DGR variable vector coincides with the IAA, Phosphate, Gibberellin, and Ammonia vectors. Ammonia stepwise regression analysis (sigα) affects DGR with an Ammonia coefficient value of 4.89; meaning that for each increase in the Ammonia value by one unit, the estimated mean value of DGR will increase by 4.89. Thus, the application of the mass based on the results of this study was able to increase the DGR value Kappaphycus alvarezii, which in turn produce the highest yield of carrageenan with K. alvarezii var.Lampung G3 was the best seed candidate to be developed at the seaweed cultivation site in Tarakan City.Keywords:AlgaePhytohormonesCarrageenanNitrogenViscosity
Identification of a Potential SNP Related to the Expression of Immune Genes and Its Possible Application to Selection of WSSV-Resistant Pacific White Shrimp (Litopenaeus vannamei) Bagus Rahmat Basuki; Alimuddin Alimuddin; Dinar Tri Soelistyowati; Sri Nuryati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.vi.39110

Abstract

Highlight Research A molecular marker for shrimp selection in disease resistance. Single nucleotide polymorphisms (SNP) in the ALF gene strongly correlate with shrimp resistance to WSSV infection. Higher ALF gene expression in survivor shrimp. SNP as molecular marker inherited in the first generation (G1) shrimp. ARMS-PCR method successfully detect SNP in the shrimp ALF gene. Abstract The Pacific white shrimp (Litopenaeus vannamei) is Indonesia's main export commodity, but its production is constrained by the white spot syndrome virus (WSSV). Selective breeding of disease-resistant broodstock based on single nucleotide polymorphism (SNP) in the anti-lipopolysaccharide factor (ALF) gene is an alternative strategy for solving the disease problem. This study aimed to detect the SNP g.455 A>G in the anti-lipopolysaccharide factor (ALF) shrimp gene, evaluate the correlation of SNP with WSSV-resistance trait, analyze the expression level of immunity genes and genotype frequencies of the WSSV-resistance population shrimp and analyze the SNP inheritance in the first generation of selected shrimp. A total of 120 individuals from 4 families were used to detect the SNP marker using tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). The correlation of the SNP marker with survival rate (SR) was analyzed using a general linear model (GLM) between genotype frequencies and SR. Genotypic similarities between broodstock and pedigree were analyzed using Chi-square. SNP g.455 A>G was successfully detected using the ARMS-PCR method and had a strong correlation between the marker and SR (p-value of AA = 0.012; AG = 0.359, and GG = 0.001). The resistant population has significantly higher ALF and SOD gene expression levels and AA genotype frequency. The SNP marker was inherited, so the broodstock and pedigree have the same genotype frequencies according to chi-square analysis (χ2 = 0.46 and p-value = 0.497). These results suggested that the g.455 genotype AA could be selected to produce WSSV-resistant Pacific white shrimp.
Feminization of Maroon Clownfish (Amphiprion biaculeatus, Bloch 1790) with 17β-Estradiol Hormonal Induction Helena Sahusilawane; Agus Oman Sudrajat; Muhmmad Agus Suprayudi; Dinar Tri Soelistyowati; Ligaya I. T. A. Tumbelaka; Irzal Effendi
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v15i2.42765

Abstract

Highlight Research The 17β-estradiol hormone induction reduces the percentage of R values in skin and fin color by as much as 50-60%. The 17β-estradiol hormone induction increases total length and body weight, estradiol content, GIS and HIS, and also glucose content. The 17β-estradiol hormone induction shows mature development of the gonadal profile from functional male to functional female. Abstract   As maroon clownfish (Amphiprion biaculeatus) is a protandrous hermaphroditic fish, feminization process with 17β-estradiol hormone can be applied to accelerate the female broodstock candidate supply for further spawning effort. This study aimed to evaluate the feminization of A. biaculeatus with 17β-estradiol hormonal induction. This study used a completely randomized design with several hormone dosage, namely P0 (without 17β-estradiol hormone induction), P1 (0.5 μg 17β-estradiol/g body), P2 (1.0 μg 17β-estradiol/g body), and P3 (1.5 μg 17β-estradiol/g body). These treatments were applied with three replications. Five fish composed of α-fish, β-fish, and three γ-fish were reared in each aquarium for 90 days with a flowing water system. The α- and β-fish were then removed, while the γ-fish was injected with hormone. Otohime pellet feed was fed three times a day until apparent satiation. The results showed that the 17β-estradiol hormone could induce 100% of the feminization process of male A. biaculeatus. The dosage of P3 obtained the lowest value percentage of red, green, blue (RGB), but showing the highest total of length and body weight (6.67±0.42 cm and 6.40±0.78 g, respectively), estradiol content (149.73±4.24 ρg/mL), GSI and HSI (0.38±0.07% and 3.59±0.49%), and glucose content (4.67±0.64 mg/dL), followed by more mature gonad profile than other treatments. This condition indicates that fish in P3 treatment has been reversed as functional female. The average survival rate for the treatment was as high as 60%. Therefore, the application of 17β-estradiol hormonal induction is effective for the feminization process in A. biaculeatus as a protandrous hermaphroditic fish.
POLA PERTUMBUHAN DAN VARIASI GENETIK BERBASIS DNA MIKROSATELIT DARI TIGA POPULASI IKAN BARAMUNDI Lates calcarifer Fitriyah Husnul Khotimah; Alimuddin Alimuddin; Dinar Tri Soelistyowati; Sri Nuryati; Harton Arfah; Ketut Sugama; Gusti Ngurah Permana; Sari Budi Moria Sembiring; Haryanti Haryanti
Jurnal Riset Akuakultur Vol 18, No 1 (2023): (Maret 2023)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.18.1.2023.49-59

Abstract

Benih ikan baramundi Lates calcarifer diperoleh dari pemijahan alami dengan jumlah induk terbatas sehingga variabilitas pertumbuhan dan kelangsungan hidup antar-batch menjadi tinggi. Penelitian dilakukan untuk menganalisis pertumbuhan dan mengevaluasi variasi genetik ikan kakap putih populasi Australia, Situbondo dan Lampung hasil domestikasi dan dibudidaya di hatcheri skala rumah tangga (HSRT). Sebanyak 10 ekor ikan barramundi dari setiap populasi digunakan untuk analisis variabilitas genetik dengan dua lokus mikrosatelit, yaitu Lca21 dan Lca32. Selanjutnya, data mikrosatelit diolah menggunakan software genetic analysis in excel (GenAlEx 6.51b2). Hasil yang diperoleh menunjukkan bahwa pertumbuhan panjang dan bobot tubuh serta laju pertumbuhan spesifik ikan baramundi dari Australia lebih tinggi dibandingkan dari Situbondo dan Lampung (P<0,05); sedangkan ikan baramundi dari Situbondo dengan Lampung adalah sama (P>0,05). Jumlah alel setiap lokus ikan baramundi berkisar 2-8 alel dan heterozigositas tertinggi dimiliki oleh ikan barramundi asal Situbondo (0,85), diikuti Lampung (0,65) dan paling rendah dari Australia (0,54). Dari hasil riset tersebut dapat disimpulkan bahwa ketiga populasi ikan baramundi hasil domestikasi dan dipeliharan dalam sistem HSRT memenuhi kelayakan untuk digunakan untuk kegiatan hibridisasi atau membentuk populasi awal (sintetis). Barramundi seeds, Lates calcarifer are usually sourced from natural spawning using a limited number of broodstock. Therefore, the growth and survival rate of these seeds vary greatly between batches. The research was performed to determine the growth pattern and genetic variations of barramundi seed populations produced from domesticated broodstock sourced from Australia, Situbondo, and Lampung and reared in small-scale hatcheries. Ten individuals of barramundi from each population were used for microsatellite analysis using two microsatellite loci, namely: Lca 21 and Lca 32. The resulted microsatellite data was processed using the genetic analysis available in Excel software (GenAlEx 6.51b2). The results showed that the growth in length and body weight as well as the specific growth rate of barramundi seeds produced from Australia broodstock were higher than that of Situbondo and Lampung (P<0.05) while the later two were similar (P>0.05). The number of microsatellite alleles ranged from 2-8 and the highest heterozygosity was obtained by barramundi seeds produced by Situbondo (0.85), followed by Lampung (0.65), dan Australia (0.54) broodstock. From the results of the research, it can be concluded that the three populations of barramundi fish, which were domesticated and reared in the HSRT system, meet the criteria for use in hybridization program or for forming a synthetic population.
INDUKSI HORMONAL PADA SEX REVERSAL UDANG GALAH (Macrobrachium rosenbergii) MENGGUNAKAN EKSTRAK SERBUK SARI PINUS (Pinus tabulaeformis) Harton Arfah; Dinar Tri Soelistyowati; Hidayatush Sholihin; Kesit Tisna Wibowo; Agus Oman Sudrajat; Odang Carman; Fajar Maulana; Alimuddin Alimuddin
Jurnal Riset Akuakultur Vol 19, No 2 (2024): Juni (2024)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.19.2.2024.85-95

Abstract

Udang galah (Macrobrachium rosenbergii) merupakan komoditas perikanan air tawar yang memiliki ciri dimorfisme seksual pada pertumbuhan individu jantan lebih cepat dibanding betina. Pembentukan populasi monoseks jantan potensial dalam budidaya bertujuan untuk meningkatkan keseragaman, memacu pertumbuhan, dan efisiensi produksi. Induksi sex reversal pada pembentukan individu neomale dapat dilakukan secara hormonal menggunakan bahan yang mengandung derivat testosteron, yaitu serbuk sari pinus (Pinus tabulaeformis). Penelitian ini bertujuan untuk mengevaluasi efektivitas ekstrak serbuk sari pinus terhadap performa sex reversal pada udang galah. Ekstrak serbuk sari pinus dengan dosis berbeda (0,0 mL L-1; 0,2 mL L-1; 0,4 mL L-1; dan 0,6 mL L-1) diberikan melalui perendaman selama 24 jam pada larva udang galah sebelum berdiferensiasi kelamin (PL15) berukuran panjang 12 ± 0,3 mm dan bobot 0,02 ± 0,001 g dengan kepadatan 60 individu per L, kemudian dipelihara selama 45 hari dalam kontainer (60 x 40 x 30 cm3) dengan tinggi air 20 cm dan kepadatan 1 individu per L. Perlakuan dosis ekstrak serbuk sari pinus dosis 0,2 mL L-1 menghasilkan nisbah kelamin jantan 46,69 ± 5,36%, sedangkan peningkatan dosis 0,4-0,6 mL L-1 dan kontrol tidak efektif mengarahkan kelamin jantan (35-42%) dengan tingkat kelangsungan hidup udang PL60 berkisar 66-82%. Ciri kelamin sekunder udang galah jantan PL60 berukuran 30-32 mm berupa male gonopore (MG) dan appendix masculine (AM), sedangkan betina memiliki appendix interna (AI) pada kaki renang kedua dan rongga di antara kedua kaki jalan kelima. Aplikasi ekstrak serbuk sari pinus pada dosis maksimal 0,2 mL L-1 dapat mengarahkan kelamin jantan udang galah.Giant freshwater prawn (Macrobrachium rosenbergii) is a freshwater fishery commodity with sexual dimorphism in which male individuals grow faster than females. The establishment of a male monosexual population is expected to increase individual uniformity, growth rate, and production efficiency. Induction of sex reversal in the formation of the pseudomale can be done hormonally using materials containing testosterone derivatives, for example, pine pollen (Pinus tabulaeformis). This study aimed to evaluate the effectiveness of pine pollen extract on the sex reversal performance of giant prawns. Pine pollen extract with different doses (0.0 mL L-1; 0.2 mL L-1; 0.4 mL L-1; and 0.6 mL L-1) was given by immersion during 24 hours in prawns before sexual differentiation (PL15) with a length of 12 ± 0.3 mm and a weight of 0.02 ± 0.001 g with a density of 60 individuals per L, then reared for 45 days in a container (60 x 40 x 30 cm3) with a water height of 20 cm and a density of 1 individual per L. Treatment with a dose of pine pollen extract at a dose of 0.2 mL L-1 resulted in a male sex ratio of 46.69 ± 5.36%, while increasing doses of 0.4-0.6 ml L-1 and the control were not effective in the sex reversal (35-42%) with a survival rate of PL60 ranged from 66-82%. The secondary sexual characteristics of PL60 male giant prawns measuring 30-32 mm are male gonopore (MG) and masculine appendix (AM), while female giant prawns have an internal appendix (AI) on the second swimming leg and the cavity between the fifth swimming leg. This study concludes that the application of pine pollen extract at a maximum dose of 0.2 mL L-1 could directly induced sex reversal of female to male of giant prawns.
EVALUASI MASKULINISASI PLATI PEDANG, Xiphophorus helleri MENGGUNAKAN KOMBINASI EKSTRAK BUAH PINUS, Pinus merkusii DENGAN AROMATASE INHIBITOR Akbar, Muhamad Saepul; Sudrajat, Agus Oman; Arfah, Harton; Soelistyowati, Dinar Tri
Media Akuakultur Vol 19, No 1 (2024): Juni 2024
Publisher : Politeknik Kelautan dan Perikanan Bone

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/ma.19.1.2024.9-15

Abstract

Hormon 17α-methyltestostrone sudah banyak digunakan untuk maskulinisasi pada kegiatan budidaya namun, bahan tersebut bersifat karsinogenik sehingga diperlukan alternatif pengganti bahan tersebut. Penelitian ini bertujuan mengevaluasi efek kombinasi ekstrak buah pinus (Pinus merkusii) dan aromatase inhibitor pada ikan plati pedang. Sebanyak 5 perlakuan menggunakan bahan berbeda, yaitu 17α-methyltestostrone, ekstrak buah pinus, aromatase inhibitor, kombinasi ekstrak buah pinus dan aromatase inhibitor, serta kontrol dengan masing-masing 3 ulangan.  Perendaman dilakukan selama 6 jam, kemudian ikan dipelihara selama 60 hari. Rasio induk jantan dan betina yang digunakan untuk pemijahan ikan pada penelitian ini adalah 1:3. Penelitian ini berfokus pada tiga parameter, yakni Tingkat Kelangsungan Hidup (TKH), Nisbah Kelamin Jantan (NKJ) dan jumlah ikan jantan yang dihasilkan pada tiap perlakuan. Hasil penelitian menunjukkan ikan yang direndam pada bahan berbeda menghasilkan nilai nisbah kelamin jantan yang lebih tinggi. Dosis terbaik pada penelitian ini adalah perendaman dengan ekstrak buah pinus dengan dosis 0,02 mL L-1.The hormone 17α-methyltestostrone has been widely used for masculinization in aquaculture, however, this material is carcinogenic and it is required to find its alternative substitutions. The aim of this research to evaluate the effects of combination of pine cone (Pinus merkusii) extract and aromatase inhibitor on swordtail fish. A total of 5 treatments using different materials, namely: 17-α methyltestosterone, pine cone extract, aromatase inhibitor, pine cone extract mixed with aromatase inhibitor, and control with 3 replications. Immersion was conducted for 6 hour and the the fish were kept for 60 days. The ratio of male to female parents used for fish spawning was 1:3. The study focused on three parameters, i.e. survival rate, male fish percentage, and number male fish produced. The results revealed fish immersed with different materials exhibited higher male fish percentage compared to the control group (not immersed). The best treatment in this research was immersion using pine cone extract with dosage of 0.02 mL L-1.
KARAKTERISTIK MORFOMETRIK DAN MERISTIC LIMA JENIS IKAN BADUT (Amphiprion sp.) DARI PULAU AMBON Helena Afia Sahusilawane; Dinar Tri Soelistyowati
Jurnal Perikanan Unram Vol 11 No 1 (2021): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v11i1.239

Abstract

Amphiprion is one of the genera in the Pomacentridae family which is dominant on coral reefs in the waters of Ambon Island. This fish has interesting morphological characteristics, unique reproductive behavior, and has a symbiosis with sea anemones as a host and has high adaptability in captivity. The diversity of species in the genus Amphiprion is high based on varying morphological characters. The purpose of this study was to characterize the morphometric and meristic characters of five types of clown fish (Amphiprion sp.) from the island of Ambon. The morphometric characteristics were measured by measuring 24 characters, while the meristic characteristics were calculated using 5 characters in parent fish Amphiprion clarkii, A. frenatus, A. ocellaris, A. percula, and A. sandaracinos which had been kept for 1 year at the Ambon Marine Aquaculture Center. The data of morphometric and meristic characteristics were evaluated using principal component analysis (PCA). The relationship between individuals was analyzed using genetic distance based on the UPGMA program from PAST software. The results of PCA analysis showed differences in morphometric characters and the five types of clownfish formed two main clusters, the first cluster consisted of A. ocellaris and A. percula and A. sandaracinos, and the second cluster consisted of A. clarkii and A. frenatus. This clustering can provide information for breeders and cultivators in the development of clown fish cultivation in breeding programs.
Co-Authors , Alimuddin , Rahman, , Achmad Sudradjat Agus Oman Sudrajat Ahmad Fahrul Syarif Ahmad Muzaki Ahmad Teduh Akbar, Muhamad Saepul Aldilla Kusumawardhani, Aldilla Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Anang Hari Kristanto Anang Hari Kristanto Aras, Annisa Khairani Arifah Ambarwati Asep Bulkini Bagus Rahmat Basuki Daniel Djokosetiyanto Dendi Hidayatullah, Dendi Deni Radona Deni Radona Dian Hardiantho Dian Hardianto Didik Ariyanto Didik Ariyanto Donny Prariska Eddy Supriyono Edison, Thomas Erlania Erlania Euis Rakhmawati Fajar Maulana . Farah Diana Fauzan, Agung Luthfi Firmansyah, Rodhi Fitriyah Husnul Khotimah Fitriyah Husnul Khotimah Flandrianto S. Palimirmo Gleni Hasan Huwoyon Gleni Hasan Huwoyon Gleni Hasan Huwoyon, Gleni Hasan Gloria I. Satriani Gloria Ika Satriani Gloria Ika Satriani Gunawan, Bambang Kusmayadi Gusti Ngurah Permana Guttifera Hafidah, Riva Hafif Syahputra Hanggara, Yudha Harton Arfah Haryanti Haryanti Haryanti Haryanti Helena Sahusilawane Herjayanto, Muh. Hidayatush Sholihin Ibrahim Satrio Faqih Ibrahim, Yusran Ida Ayu Amarilia Dewi Murni Imron Imron, Imron Indah Mastuti Intan Putriana Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini, Irin Iriana Irwan Irwan Irzal Effendi Iskandariah Iskandariah Iskandariah, Iskandariah Kesit Tisna Wibowo Ketut Mahardika Ketut Sugama Ketut Sugama Komar Sumantadinata Kukuh Nirmala Ligaya I. T. A. Tumbelaka M. H. Fariduddin Ath-thar M. Syukur M. Zairin Junior Mahardhika, Prana Mahdaliana, Mahdaliana Melta Rini Fahmi MH. Fariduddin Ath-thar Mia Setiawati Muh. Herjayanto Muhamad Syukur Muhammad Fadlan Furqon Muhammad Hunaina Fariduddin Ath-thar Muhammad Sami Daryanto Muhammad Zairin Jr Muhammad Zairin Jr. Muhmmad Agus Suprayudi Muliari Muliari Mulyasari Mulyasari Nopri Yanto Odang Carman Oktaviani, Tia Peni Pitriani Poppy Dea Bertha, Poppy Dea Prana Mahardhika Prassetyo Dwi Dhany Wijaya Rahmadani Rahmadani Ratu Siti Aliah Raudhatus Sa'adah Rezki, Dinda Wahyu RIDWAN AFFANDI Rinaldi Rinaldi Rini Susilowati Riva Hafidah Rizki Eka Puteri Ronny Rachman Noor Ruby Vidia Kusumah Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Safira Qisthina Ayuningtyas, Safira Qisthina Sahusilawane, Helena Afia Sandra, Aan Aryanti Sari Budi Moria Sembiring Sari Budi Moria Sembiring Selly Ratna Sari Sri Nuryati Sri Sundari Sudarto Sudarto Syahril, Alfis Tia Oktaviani Upmal Deswira Vitas Atmadi Prakoso Wahyutomo Wahyutomo Wahyutomo, Wahyutomo Wicaksono, Aryo Wenang WIDANARNI WIDANARNI Wildan Nurussalam Wiyoto Wiyoto Yeni Elisdiana Yogi Himawan