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All Journal Jurnal Akuakultur Indonesia Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia BERITA BIOLOGI Jurnal Akuatika Indonesia Omni-Akuatika Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Sains Natural: Journal of Biology and Chemistry Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Tolis Ilmiah: Jurnal Penelitian Clarias : Jurnal Perikanan Air Tawar Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine Jurnal Perikanan

Dinar Tri Soelistyowati

Department Of Aquaculture, Faculty Of Fisheries And Marine Sciences, IPB University Bogor, West Java, 16680. Indonesia

Author-ID : 383905

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Social Sciences

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SNP2-Lys-C markers inheritance, genes expression, and the resistance of second-generation Catfish Clarias gariepinus against Aeromonas hydrophila infection Hanggara, Yudha; Alimuddin, Alimuddin; Tri Soelistyowati, Dinar Tri Soelistyowati; Nuryati, Sri
Jurnal Akuakultur Indonesia Vol. 24 No. 1 (2025): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.24.1.1-9

High mortality rates in catfish seed caused by motile Aeromonas septicemia disease caused by Aeromonas hydrophila infection. The primary focus is on the genetic selection of the second generation (G2) catfish for resistance to A. hydrophila, utilizing the type-C lysozyme gene at SNP-2 genotype TT. The objective is to assess the inheritance of the SNP2-Lys-C genetic marker from the first generation (G1) Sangkuriang breeders and to evaluate the resistance of the G2 seed to infection. In the experiment, G1 breeders with the TT genotype were spawned, and the resulting G2 seed were challenged with A. hydrophila. The findings indicate that all G2 seed possess the TT genotype and exhibit a higher survival rate compared to the dominant CT genotype seed. The expression of the Lys-C gene in G2 seed is elevated at the onset of infection, decreasing after the seventh day. The expression of the MHC1a and IL-1b genes is also associated with a higher survival rate. The survival of G2 seed correlates with the white blood cell and lysozyme activity, along with an increase in phagocytic activity peaking on the seventh day post-challenge. The conclusion of this study suggests that G2 catfish seed with the TT genotype have stronger resistance to A. hydrophila infection compared to seed with the dominant CT genotype. Keywords: Aeromonas hydrophila, catfish, genotype, lysozyme, resistance ABSTRAK Mortalitas tinggi yang terjadi pada benih lele yang disebabkan oleh penyakit motile aeromonas septicemia akibat Aeromonas hydrophila. Fokus utama adalah pada seleksi genetik generasi kedua (G2) lele untuk resistensi terhadap A. hydrophila, menggunakan gen lisozim tipe-C pada SNP-2 genotipe TT. Studi ini bertujuan menilai pewarisan marka genetik SNP2-Lys-C dari induk sangkuriang generasi pertama (G1) dan mengevaluasi ketahanan benih G2 terhadap infeksi. Dalam eksperimen, induk G1 genotipe TT dipijahkan dan benih G2 yang dihasilkan diuji dengan bakteri A. hydrophila. Hasil menunjukkan bahwa semua benih G2 memiliki genotipe TT dan menunjukkan tingkat kelangsungan hidup yang lebih baik dibandingkan dengan benih dominan genotipe CT. Ekspresi gen Lys-C pada benih G2 tinggi pada awal infeksi, menurun setelah hari ketujuh. Ekspresi gen MHC1a dan IL-1b juga terkait dengan tingkat kelangsungan hidup yang lebih tinggi. Kelangsungan hidup benih G2 berkorelasi dengan jumlah sel darah putih dan aktivitas lisozim, serta peningkatan aktivitas fagositik yang mencapai puncak pada hari ketujuh. Kesimpulan studi ini menunjukkan bahwa benih lele G2 dengan genotipe TT memiliki resistensi yang lebih kuat terhadap infeksi A. hydrophila dibandingkan dengan benih dominan genotipe CT. Kata Kunci: Aeromonas hydrophila, genotipe, ikan lele, lisozim, resistan

SEX REVERSAL IKAN NILA (Oreochromis Niloticus) MENGGUNAKAN HORMON 17Î±-METILTESTOSTERON DENGAN FREKUENSI PERENDAMAN BERBEDA Rinaldi, Rinaldi; Soelistyowati, Dinar Tri; Imron, Imron; Baihaqi, Baihaqi; Muliari, Muliari; Yanto, Nopri
Tolis Ilmiah : Jurnal Penelitian Vol 4 No 2: November
Publisher : LPPM Universitas Madako Tolitoli

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56630/jti.v4i2.291

Jantanisasi pada ikan nila sangat penting dilakukan untuk mengendalikan reproduksi secara liar dan mengoptimalkan pertumbuhan ikan jantan yang secara seksual dimorfisme lebih tinggi dibandingkan betina. Penelitian ini menggunakan rancangan acak lengkap MT dosis rendah 500 Âµg/ L. Pada penelitian menggunakan 17Î±-MT dosis 500 Âµg L-1 dengan frekuensi perendaman dua kali menghasilkan nisbah kelamin jantan sebanyak 87.04% dan pada perendaman tiga kali mengalami penurunan sebesar 4.63%. Kelangsungan hidup dan pertumbuhan ikan tidak berbeda nyata antara ikan kontrol dengan ikan perlakuan.Kata Kunci: Jantanisasi, Ikan nila, 17 alpha Methyltestosterone

Masculinization on tilapia (Oreochromis sp.) by natural hormone steroid agent from bee resin through artificial diet Soelistyowati, Dinar Tri; Sudrajat, Agus Oman; Arfah, Harton
Jurnal Akuakultur Indonesia Vol. 9 No. 2 (2010): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Masculinization on tilapia ( Oreochromis sp.) has been done with approach of natural hormone steroid agent from bee resin through artificial diet before sexual differentiation. This research aim to increase male sex ratio and production performance. Treatment of bee resin dose is 0.6; 1.2; 1.8; 2.4; 3.0 feed ml/kg compared to Control (0 ml/kg diet) were given during 28 days at larva age 7 day in aquarium, then transferred to the farm ground until age 12 weeks. Average of masculine phenotype percentage increased significantly (P

Phenotype characterization of interspecific hybrid abalone Haliotis asinina and Haliotis squamata seed Soelistyowati, Dinar Tri; Kusumawardhani, Aldilla; Junior, Muhammad Zairin
Jurnal Akuakultur Indonesia Vol. 12 No. 1 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Abalone is one of sea-water aquaculture commodity that having relatively low in growth and survival. Interspesific hybridization between abalone Haliotis asinina and Haliotis squamata is required to produce hybrid seeds having a better phenotype inherited from their parents. Crossbreeding of abalone was done in the reciprocal procedure with a natural spawning technique on mass scale. The hybrid seeds showed higher similarity with female brood (98,69%), while the larvae from H. squamata × H. asinina were abnormal on trocophore until early veliger phase then dead occurred the next phase. The results showed that hybridization between male H. asinina and female H. squamata had higher fertilization and hatching rate than its reciprocal i.e. 76.01±6.15% and 60.14±4.80%. Keywords: interspesific hybridization, phenotype, Haliotis asinina, Haliotis squamata, abalone ABSTRAK Abalon merupakan komoditas budidaya laut dengan pertumbuhan yang relatif lambat dan kelangsungan hidupnya rendah. Rekayasa persilangan interspesifik antara abalon Haliotis asinina dan Haliotis squamata diharapkan mampu mengatasi permasalahan benih dan memiliki fenotipe unggul yang diwariskan dari tetuanya. Persilangan abalon dilakukan secara resiprok dengan teknik pemijahan alami skala massal. Hasil penelitian menunjukkan bahwa benih hibrida menunjukkan kemiripan dengan induknya sebesar 98,69%, sedangkan larva hibrida antara jantan H. squamata × H. asinina menunjukkan bentuk abnormal pada fase trokofor hingga veliger awal dan kematian pada fase lanjut. Hibridisasi antara jantan H. asinina dan betina H. squamata memiliki derajat pembuahan dan derajat penetasan yang lebih tinggi dibandingkan resiprokalnya, yaitu berturut-turut 76,01±6,15% dan 60,14±4,80%. Kata kunci: hibridisasi interspesifik, fenotipe, Haliotis asinina, Haliotis squamata, abalon

Genetic variability of the fifth generation of nile tilapia Oreochromis niloticus using microsatellite DNA markers Satriani, Gloria Ika; Soelistyowati, Dinar Tri; Hardianto, Dian; Aliah, Ratu Siti
Jurnal Akuakultur Indonesia Vol. 10 No. 2 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTFifth generations of Nile tilapia from several strains have been produced by using selective breeding program in Main Centre for Freshwater Aquaculture Development (MCFAD) Sukabumi, West Java. This research was aimed to evaluate the impact of family selection program of some highly economic traits on its genetic variability using microsatellite DNA markers. The total of 180 specimens have been collected from fifth generation of nine reciprocal mating between three families selected from fourth generation of Nile tilapia and were screened for genetic variability at three microsatellite loci (UNH 123*, UNH 172*, UNH 216*). The results showed that the amount of genetic variability on fifth generations of Nile tilapia from three strains was ranged between 33 to 100% and the highest genetic distance relationship between families was 0.3875. This research approved that females and males issued from the family which have more amount of genetic variability and higher distance to others could be considered as genetic materials to produce the next generation.Keywords: microsatellite DNA, genotype, genetic variability, genetic distance, Oreochromis nilotiocus ABSTRAKBeberapa strain ikan nila generasi kelima telah dihasilkan dalam program pemuliaan di Main Centre untuk Freshwater Aquaculture Development (MCFAD) Sukabumi, Barat Jawa. Riset ini bertujuan untuk mengevaluasi pengaruh seleksi famili terhadap performa karakter ekonomis penting berdasarkan keragaman genetiknya menggunakan penanda microsatellite DNA. Spesimen dari 180 individu generasi kelima hasil persilangan resiprokal antara tiga famili generasi keempat dianalisis dengan penanda tiga microsatellite loci (UNH 123*, UNH 172*, UNH 216*). Hasil penelitian menunjukkan bahwa keragaman genetik ikan nila generasi kelima berkisar antara 33 sampai 100% dan hubungan kekerabatan genetik antar famili yang paling jauh adalah 0,3875. Individu betina dan jantan yang berasal dari famili dengan tingkat keragaman genetik dan kekerabatan yang lebih tinggi dapat dipertimbangkan sebagai sumber genetik berkualitas untuk menghasilkan generasi berikutnya.Kata kunci: microsatellite DNA, genotipe, keragaman genetik, jarak genetik, Oreochromis nilotiocus

Masculinization of betta fish Betta splendens by embryo immersion in extract of purwoceng Pimpinella alpina Arfah, Harton; Soelistyowati, Dinar Tri; Bulkini, Asep
Jurnal Akuakultur Indonesia Vol. 12 No. 2 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This study aimed to examine the effect of extract of purwoceng Pimpinella alpina for masculinization of Betta splendens. This research used completely randomized design with three treatments that were distinguished by doses of purwoceng extract, which were 10, 20, and 30 µL/L, and 0 µL/L as control. The treatments were given by immersion to 35 embryos at eye spots phase or about 28-hours after fertilization, for eight hours. The immersion process was done for 8 hours. The result showed that 20 µL/L dose of purwoceng extract produced 62.66% male, and hatching rate was 85.71%. This male percentage was higher compared to control (45.91%), but the hatching rate was lower than that of control (98.57%). At the higher dose (30 µL/L), male fish population and hatching rate reduced respectively 39.72% and 68.57%, respectively. Keywords: masculinization, embryo immersion, purwoceng extract, Betta splendens ABSTRAK Penelitian ini bertujuan untuk mengevaluasi pengaruh dosis ekstrak purwoceng Pimpinella alpina extract dalam maskulinisasi ikan cupang hias Betta splendens. Penelitian ini menggunakan metode rancangan acak lengkap dengan tiga perlakuan dan tiga ulangan dosis ekstrak purwoceng. yaitu 10, 20, dan 30 µL/L, serta perlakuan 0 µL/L sebagai kontrol. Perlakuan diberikan melalui perendaman selama delapan jam terhadap 35 embrio saat memasuki fase bintik mata atau sekitar jam ke-28 setelah pembuahan. Hasil penelitian menunjukkan bahwa dosis ekstrak purwoceng 20 µL/L menghasilkan persentase ikan jantan sebesar 62,66%, dan daya tetas telur sebesar 85,71%. Persentase jantan tersebut lebih tinggi daripada kontrol (45,1%), tetapi derajat penetasannya lebih rendah daripada kontrol (98,57%). Pada dosis yang lebih tinggi (30 µL/L), populasi ikan jantan (39,72%), dan derajat penetasan (68,57%) adalah menurun. Kata kunci: maskulinisasi, perendaman embrio, ekstrak purwoceng, Betta splendens

Gracilaria spp. morphology cultured in brackish water pond Pantai Sederhana Village, Muara Gembong Soelistyowati, Dinar Tri; Murni, Ida Ayu Amarilia Dewi; Wiyoto, ,
Jurnal Akuakultur Indonesia Vol. 13 No. 1 (2014): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Gracilaria spp. is a euryhaline species of seaweed which can live in the marine and brackish water. Development of Gracilaria spp. culture in Bekasi is potential because this seaweed can be cultured in ex shrimp pond by polyculture system. The objective of this research was to evaluate the phenotype morphological characteristic of Gracilaria spp. based on and its relationship with shrimp pond water quality. Sampling was done at three shrimp ponds with a salinity range at 13.7–19.2 g/L. Phenotypical characteristics of Gracilaria spp. consisted of colour and thallus morfometrics, while measurement of water quality consisted of physical and chemical charactersof shrimp pond. The result showed that Gracilaria spp. generally had light brown colour. At salinity higher than 13.7 g/kg, the number of secondary thalli increased, the distance among internode tertiary thalli declined, and the number of ramification index increased. Salinity showed a positive correlation with remification index which was 0.571. Keywords: Gracilaria spp., remification index, phenotype, salinity, brackishwater culture ABSTRAK Gracilaria spp. merupakan spesies rumput laut eurihalin yang dapat hidup di laut dan di perairan payau. Pengembangan budidaya Gracilaria spp. di Bekasi potensial dilakukan karena memanfaatkan tambak bekas budidaya udang dengan sistem polikultur. Penelitian ini bertujuan untuk mengevaluasi karakteristik fenotipe morfologi Gracilaria spp. dan hubungannya dengan kualitas air di tambak budidaya. Sampling dilakukan pada tiga tambak dengan kisaran salinitas 13,7–19,2 g/L. Karakterisasi fenotipe meliputi warna dan morfometrik talus Gracilaria spp., sedangkan parameter kualitas air meliputi karakter fisika dan kimia air tambak. Hasil menunjukkan talus Gracilarias spp. umumnya berwarna coklat muda dan pada salinitas di atas 13,7 g/L menunjukkan jumlah talus sekunder meningkat, jarak internode talus tersier menurun, dan indeks percabangan meningkat (P<0,05). Salinitas berkorelasi positif dengan indeks percabangan sebesar 0,571. Kata kunci: Gracilaria spp., indeks percabangan, fenotipe, salinitas, budidaya air payau

Sex reversal of red tilapia using 17α-methyltestosterone-enriched feed and increased temperature Ayuningtyas, Safira Qisthina; Junior, Muhammad Zairin; Soelistyowati, Dinar Tri
Jurnal Akuakultur Indonesia Vol. 14 No. 2 (2015): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The growth rate between male and female red tilapia Oreochromis sp. is different. Generally, the male red tilapia grows faster than the female. Furthermore, the maturation process of red tilapia is relatively fast which causes slower growth rate. One of solutions to this problem is by rearing all male population or mono-sex culture. The method used in this study was commercial feed enrichment with 17α-methyltestosterone at different dosages and water temperature manipulation. The purpose of this research was to examine the effects of commercial feed enrichment with different dosages of 17α-methyltestosterone and water temperature manipulation on success rate of sex reversal of red tilapia into all male population. This research consisted of different temperature treatments (with and without water heating) and 17α- methyltestosterone dosages (0, 10, 20 mg/kg of commercial feed). The best dosage of 17α-methyltestosterone was 20 mg/kg of commercial feed with male to female sex ratio of 86.31%, daily growth rate of 8.18%, and feed conversion ratio of 1.53. In this study, the best treatment to produce the male seeds was the 17α-methyltestosterone treatment. Keywords: 17α-methyltestosterone, sex reversal, red tilapia, temperature ABSTRAK Ikan nila merah Oreochromis sp. memiliki laju pertumbuhan yang berbeda antara ikan jantan dan betina. Umumnya ikan nila merah jantan lebih cepat tumbuh dibandingkan betinanya. Selain itu, ikan nila memiliki sifat cepat matang gonad dan mudah memijah sehingga akan menghambat pertumbuhan ikan. Salah satu cara untuk mengurangi masalah yang terjadi yakni dengan memelihara populasi ikan nila merah tunggal kelamin atau monoseks jantan. Metode yang dilakukan adalah pemberian hormon 17α-metiltestosteron dengan dosis berbeda melalui pakan buatan dan peningkatan suhu air. Penelitian ini bertujuan untuk mengetahui pengaruh dosis hormon 17α-metiltestosteron melalui pakan buatan dan peningkatan suhu air terhadap keberhasilan alih kelamin jantan pada ikan nila merah. Penelitian ini terdiri atas perlakuan suhu (dengan dan tanpa pemanasan air) dan dosis 17α-metiltestosteron (0, 10, 20 mg/kg pakan). Dosis hormon 17α-metiltestosteron terbaik yang didapatkan adalah 20 mg/kg pakan dengan nisbah kelamin jantan 86,31%, laju pertumbuhan harian 8,18%, dan rasio konversi pakan 1,53. Pada penelitian ini perlakuan terbaik untuk menghasilkan benih jantan adalah perlakuan dosis 17α-metiltestosteron. Kata kunci: 17α-metiltestosteron, alih kelamin, ikan nila merah, suhu

Spermatogenesis of male catfish Clarias sp. fed diet supplemeted with purwoceng extract Bertha, Poppy Dea; Junior, Muhammad Zairin; Soelistyowati, Dinar Tri
Jurnal Akuakultur Indonesia Vol. 15 No. 1 (2016): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Purwoceng Pimpinella alpina Molk has been a commercial medicinal plant that their root could be used as aphrodisiac, diuretic, and body fit enhancer. This research was performed to evaluated the effect of purwoceng on spermatogenesis of the male catfish Clarias sp. consisted of testis weight, gonado somatic index (GSI), and spermatocrite levels. The treatment comprised administering purwoceng extract through the feed at a dose of 0; 2.5; 5; 7.5 g/kg of feed. Experimental fish used were male catfish Clarias sp. with initial body weight of 200–300 g at the density of 10 fish/tank. Male catfish were maintained in tank sized 2×1,5×1 m3, filled with water at 60–70 cm deep. The result showed that purwoceng extract at a dose of 5 g/kg mixed in the feed increased testis weight, GSI values, and spermatocrite levels in adult male catfish. Keywords: purwoceng extract, spermatogenesis, catfish ABSTRAK Purwoceng Pimpinella alpina Molk merupakan tanaman herbal komersial yang akarnya dilaporkan berkhasiat sebagai obat afrodisiak, diuretik, dan tonik. Penelitian ini bertujuan untuk mengevaluasi pengaruh ekstrak purwoceng terhadap perkembangan testis ikan lele Clarias sp. yang meliputi peningkatan bobot testis, nilai GSI, serta nilai spermatokrit. Perlakuan terdiri atas pemberian ekstrak purwoceng melalui pakan dengan dosis 0; 2,5; 5 dan 7,5 g/kg pakan. Adapun ikan yang diujicobakan pada penelitian adalah ikan lele jantan dengan bobot awal 200–300 g dan padat tebar 10 ekor/bak. Pemeliharaan ikan dilakukan pada bak berukuran 2×1,5×1 m3 yang diisi air dengan ketinggian 60–70 cm. Hasil percobaan menunjukkan bahwa pemberian ekstrak purwoceng dengan dosis 5 g/kg yang dicampur dalam pakan meningkatkan nilai bobot testis, nilai GSI dan kadar spermatokrit pada lele jantan dewasa. Kata kunci: ekstrak purwoceng, spermatogenesis, ikan lele

Genetic variability and performance of Asian swamp eel Monopterus albus (Zuiew, 1793) from West Java cultured in saline water medium Soelistyowati, Dinar Tri; Syarif, Ahmad Fahrul; Affandi, Ridwan; Hidayatullah, Dendi
Jurnal Akuakultur Indonesia Vol. 16 No. 1 (2017): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Asian swamp eel Monopterus albus (Zuiew, 1793) is freshwater fish species which is prospective for domestic and export markets. The production is limited depend on the catches of natural population. The cultivation of eel has been carried out to increase the production for sustainability. This study was conducted to evaluate the genetic variability and performance of Asian swamp eel from West Java and its potential cultivation in water based media with salinity. Three populations from West Java were collected of different altitudes from Sukabumi (673 m asl), Cianjur (429 m asl), Karawang (51 m asl) sized 19–26.5 cm and weighed 4.95–11.4 g. The cultivation was performed during 30 days in water media without substrate at salinity 6 ppt with density of 1 kg/m2 and maintenance at container 50×30×30 cm completed with shelter pipe of ¾ inches diameter and 20 cm length, height of water 10 cm and water exchange 100% every day, fed at satiation using Tubificidae once a day. Genetically, all of the populations showed low heterozygosity at 1.19–1.23% and genetic distance 0.01–0.04. Asian swamp eel adapted better at water salinity 6 ppt which indicated by low mesure of osmotic gradient and blood glucose. Asian swamp eel from Karawang showed superior at survival rate (90%) and daily growth rate (1.42/day). Keywords: Asian swamp eel, Monopterus albus, cultivation, genetic variability, salinity ABSTRAK Belut sawah Monopterus albus (Zuiew, 1793) merupakan komoditas ikan air tawar potensial di pasar domestik maupun ekspor, namun produksinya masih mengandalkan hasil tangkapan dari alam karena budidaya belum berkembang. Penelitian ini bertujuan untuk mengevaluasi keragaman genetik dan keragaan belut sawah asal Jawa Barat dan potensinya untuk dikembangkan dengan teknik budidaya di air bersalinitas tanpa lumpur. Tiga populasi belut sawah dikoleksi dari lokasi di Jawa Barat dengan ketinggian berbeda yaitu Sukabumi (673 m dpl), Cianjur (429 m dpl), Karawang (51 m dpl). Sumber genetik belut berukuran 19–26,5 cm dan bobot berkisar 4,95–11,4 g dipelihara selama 30 hari dalam media air tanpa substrat bersalinitas 6 ppt. Wadah pemeliharaan berukuran 50×30×30 cm dilengkapi shelter pipa paralon diameter ¾ inci dan panjang 20 cm serta ketinggian air 10 cm. Padat penebaran ikan 1 kg/m2 (20 ekor/wadah), serta pergantian air 100% dilakukan setiap hari dan pemberian pakan berupa Tubificidae secara at satiation satu kali sehari. Secara genetik ketiga populasi menunjukkan tingkat heterosigositas yang rendah yaitu berkisar 0,19–0,23 % dan jarak genetik 0,01–0,04. Belut sawah menunjukkan respons adaptasi yang baik dalam media air tanpa substrat pada salinitas 6 ppt berdasarkan indikator beban osmotik dan kadar glukosa yang rendah. Belut sawah asal Karawang unggul pada laju pertumbuhan harian (1,42/hari) dan kelangsungan hidup (90 %). Kata kunci: belut sawah Monopterus albus, budidaya, keragaman genetik, salinitas

Co-Authors , Alimuddin , Rahman, , Achmad Sudradjat Agus Oman Sudrajat Ahmad Fahrul Syarif Ahmad Muzaki Ahmad Teduh Akbar, Muhamad Saepul Aldilla Kusumawardhani, Aldilla Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Anang Hari Kristanto Anang Hari Kristanto Aras, Annisa Khairani Arifah Ambarwati Asep Bulkini Bagus Rahmat Basuki Daniel Djokosetiyanto Dendi Hidayatullah, Dendi Deni Radona Deni Radona Dian Hardiantho Dian Hardianto Didik Ariyanto Didik Ariyanto Donny Prariska Eddy Supriyono Edison, Thomas Erlania Erlania Euis Rakhmawati Fajar Maulana . Farah Diana Fauzan, Agung Luthfi Firmansyah, Rodhi Fitriyah Husnul Khotimah Fitriyah Husnul Khotimah Flandrianto S. Palimirmo Gleni Hasan Huwoyon Gleni Hasan Huwoyon Gleni Hasan Huwoyon, Gleni Hasan Gloria I. Satriani Gloria Ika Satriani Gloria Ika Satriani Gunawan, Bambang Kusmayadi Gusti Ngurah Permana Guttifera Hafidah, Riva Hafif Syahputra Hanggara, Yudha Harton Arfah Haryanti Haryanti Haryanti Haryanti Helena Sahusilawane Herjayanto, Muh. Hidayatush Sholihin Ibrahim Satrio Faqih Ibrahim, Yusran Ida Ayu Amarilia Dewi Murni Imron Imron, Imron Indah Mastuti Intan Putriana Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini, Irin Iriana Irwan Irwan Irzal Effendi Iskandariah Iskandariah Iskandariah, Iskandariah Kesit Tisna Wibowo Ketut Mahardika Ketut Sugama Ketut Sugama Komar Sumantadinata Kukuh Nirmala Ligaya I. T. A. Tumbelaka M. H. Fariduddin Ath-thar M. Syukur M. Zairin Junior Mahardhika, Prana Mahdaliana, Mahdaliana Melta Rini Fahmi MH. Fariduddin Ath-thar Mia Setiawati Muh. Herjayanto Muhamad Syukur Muhammad Fadlan Furqon Muhammad Hunaina Fariduddin Ath-thar Muhammad Sami Daryanto Muhammad Zairin Jr Muhammad Zairin Jr. Muhmmad Agus Suprayudi Muliari Muliari Mulyasari Mulyasari Nopri Yanto Odang Carman Oktaviani, Tia Peni Pitriani Poppy Dea Bertha, Poppy Dea Prana Mahardhika Prassetyo Dwi Dhany Wijaya Rahmadani Rahmadani Ratu Siti Aliah Raudhatus Sa'adah Rezki, Dinda Wahyu RIDWAN AFFANDI Rinaldi Rinaldi Rini Susilowati Riva Hafidah Rizki Eka Puteri Ronny Rachman Noor Ruby Vidia Kusumah Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Safira Qisthina Ayuningtyas, Safira Qisthina Sahusilawane, Helena Afia Sandra, Aan Aryanti Sari Budi Moria Sembiring Sari Budi Moria Sembiring Selly Ratna Sari Sri Nuryati Sri Sundari Sudarto Sudarto Syahril, Alfis Tia Oktaviani Upmal Deswira Vitas Atmadi Prakoso Wahyutomo Wahyutomo Wahyutomo, Wahyutomo Wicaksono, Aryo Wenang WIDANARNI WIDANARNI Wildan Nurussalam Wiyoto Wiyoto Yeni Elisdiana Yogi Himawan

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