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All Journal Jurnal Akuakultur Indonesia Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia BERITA BIOLOGI Jurnal Akuatika Indonesia Omni-Akuatika Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Sains Natural: Journal of Biology and Chemistry Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Tolis Ilmiah: Jurnal Penelitian Clarias : Jurnal Perikanan Air Tawar Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine Jurnal Perikanan

Dinar Tri Soelistyowati

Department Of Aquaculture, Faculty Of Fisheries And Marine Sciences, IPB University Bogor, West Java, 16680. Indonesia

Author-ID : 383905

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Social Sciences

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SPAWNING OF BLACK GHOST KNIFEFISH, Apteronotus albifrons WITH DIFFERENT SEX RATIOS Fajar Maulana; Dinar Tri Soelistyowati; Muhammad Fadlan Furqon
Indonesian Aquaculture Journal Vol 16, No 1 (2021): (June, 2021)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.16.1.2021.29-34

Black ghost knifefish, Apteronotus albifrons, is a South America-introduced ornamental fish species that has been widely cultivated in Indonesia. Some farmers breed this fish with different sex ratios, but the optimum sex ratio remains unclear. This study aimed to evaluate the spawning behavior and reproductive performance of black ghost knifefish with different sex ratios. The treatments in this study were arranged in a completely randomized design consisting of different sex ratios between males and females, namely: A (one male : three females), B (two males : three females), and C (three males : three females). Each treatment was done in triplicate. The broodstock were maintained in an aquarium (80 cm x 40 cm x 40 cm) and fed with bloodworm twice a day. The water was changed every day as much as 60% of the total volume. During the experiment, the parameters of spawning behavior, number of fish spawning, number of eggs, fertilization rate, hatching rate, and daily spawning frequencies were observed. The observation was done for seven days. The study results showed that black ghost knifefish spawned at night (11 pm - 2 am). The mating and spawning occurred between one male and one female. Competition between males was observed in the treatments indicated by aggressive movements of a male toward the others, such as sudden approaching, chasing, and driving away the others. Fish in treatment-B spawned consistently from day-1 to day-5. Fish in treatment-A spawned from day-2 to day-4, while fish in treatment-C spawned only on day-7. The reproductive performance parameters showed no significant difference in all treatments except the hatching rate parameter of treatment-B. The study concludes that better reproductive performance of black ghost knifefish, A. albifrons can be achieved with a spawning ratio of two males and three females. Further research on individual and mass spawning methods with the best spawning ratio of the fish is required.

VARIATIONS OF RED COLOR COVERAGE OF CULTURED NEON TETRA (Paracheirodon innesi) FOR BREEDING IMPROVEMENT STRATEGIES Ruby Vidia Kusumah; Dinar Tri Soelistyowati; Alimuddin Alimuddin; Melta Rini Fahmi
Indonesian Aquaculture Journal Vol 16, No 1 (2021): (June, 2021)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.16.1.2021.1-11

Red color coverage (RCC) is a commercial trait developed and refined to improve the appearance of many ornamental fish commodities. In neon tetra, the status of variation of RCC is not yet investigated or reported. This study aimed to analyze the RCC variation of cultured neon tetra as a basis for breeding strategies. A total of 900 neon tetras (standard length, SL, of 2.29 ± 0.16 cm) were collected from Bojongsari, Curug, and Pondok Petir fish farms located in Depok Districts, West Java. All fish were relocated and reared in a fish farm specialized in culturing neon tetra for two weeks using nine aquariums with photoperiod set up of 12 hours bright and 12 hours dark. The RCC traits were determined according to the percentages of RCC length (%LRCC), RCC width (%WRCC), and RCC area (%ARCC) and quantified using the digital image method. The result showed that the RCC varied by sex, size, and original location (P<0.05) in a low coefficient of variation (1.89%-11.41%). The RCC values in the male group were higher than that of females based on %LRCC and %ARCC parameters (P<0.05). Males had the highest %LRCC at size LXL, which was correlated with SL (r 0.25, P<0.1), of females at M size. The %LRCC values of the neon tetra population from the Bojongsari farm were higher than those from the other locations. Based on these findings, breeding strategies of the RCC traits should consider sex, size, and population (farm location) variations. Specifically for neon tetra, this strategy should be based on selecting the SL or %LRCC parameter of M for females and LXL for males.

ANALISIS KUALITAS RUMPUT LAUT Gracilaria gigas YANG DIBUDIDAYA PADA HABITAT LAUT DAN TAMBAK, NUSA TENGGARA BARAT Farah Diana; Kukuh Nirmala; Dinar Tri Soelistyowati
Jurnal Riset Akuakultur Vol 9, No 1 (2014): (April 2014)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Gracilaria gigas merupakan tumbuhan tingkat rendah yang berasal dari laut danumumnya dibudidaya di tambak. Pengembangan budidaya rumput laut di tambak bertujuan memperluas kawasan produksi dengan produktivitas yang tinggi, kualitas rumput laut dan agar yang berbeda dengan di laut. Budidaya Gracilaria gigas di tambak dilakukan di Sekotong, Lombok Barat, Nusa Tenggara Barat dengan menggunakan metode broadcast dengan luas area budidaya 1.500 m2. Sedangkan budidaya di laut dilakukan di Teluk Gerupuk, Lombok Tengah dengan metode long line dengan luas area budidaya 1.250 m2. Parameter yang diukur meliputi performa dan kualitas rumput laut, serta kualitas air. Parameter kualitas air yang diukur adalah: suhu, salinitas, pH, NO3-N, NO2-N, NH3-N, PO4-P, dan kecerahan yang diambil pada hari ke-0, 10, 20, dan 30. Rata-rata produktivitas Gracilaria gigas di laut lebih tinggi 12,72% daripada di tambak. Sebaliknya rendemen agar dan kekuatan gel Gracilaria gigas hasil budidaya di tambak hampir tiga kali lipat lebih tinggi daripada di laut dan berkorelasi positifdengan kandungan N perairan dan indeks percabangan. Kualitas rumput laut berhubungan erat dengan suhu, DO, PO4-P, dan NH3-N terlarut dalam air. Tingginya rendemen agar dan kekuatan gel di tambak disebabkan oleh banyaknya kandungan nutrien dan unsur hara, sedangkan tingginya produktivitas hasil budidaya Gracilaria gigas di laut disebabkan oleh adanya respons struktural dan tekanan turgor pada rumput laut.

RAGAM GENOTIPE IKAN TENGADAK, Barbonymus schwanenfeldii (Bleeker 1854) PERSILANGAN POPULASI JAWA DAN KALIMANTAN BERDASARKAN RAPD Deni Radona; Dinar Tri Soelistyowati; Rudhy Gustiano; Odang Carman; Irin Iriana Kusmini; Sri Sundari
Jurnal Riset Akuakultur Vol 11, No 2 (2016): (Juni 2016)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Dalam rangka pengelolaan sumber genetik untuk pengembangan budidaya ikan tengadak maka perlu dilakukan evaluasi sumber genetik ikan tengadak asal Jawa dan Kalimantan, serta progeni hibridanya. Analisis genetik dilakukan secara molekuler dengan metode RAPD. Jumlah sampel yang digunakan untuk analisis RAPD sebanyak 10 ekor setiap populasi. Spesimen yang digunakan untuk analisis RAPD adalah sirip untuk induk dan whole body untuk hibrida. Hasil menunjukkan polimorfisme (32,43%) dan heterozigositas (0,13) tertinggi terdapat pada ikan tengadak hasil persilangan betina Jawa x jantan Kalimantan, sedangkan yang terendah diperoleh pada persilangan betina Kalimantan x jantan Jawa (polimorfisme: 21,62% dan heterozigositas: 0,10). Berdasarkan dendrogram hubungan kekerabatan interpopulasi ikan tengadak hasil persilangan (betina Kalimantan x jantan Jawa) dengan induknya (populasi Jawa dan Kalimantan) menggunakan tiga primer RAPD (OPA-08, OPA-09, dan OPC-02) menunjukkan jarak genetik berkisar 0,48. Ikan tengadak betina asal Jawa dan jantan asal Kalimantan potensial meningkatkan keragaman genetik.In order to maintain the genetic sources of tinfoil barb for aquaculture development, it is necessary to evaluate the genetic diversity crossbred results of tinfoil barb from Java and Kalimantan. The genetic assessment was conducted by genotype trails using RAPD methods. The samples used for the analysis of RAPD was as much as 10 individuals. Specimens used for RAPD analysis was a fin for broodstock and whole body for the hybrid fish. The result showed that the highest polymorphism and heterozygosity were found 32.43% and 0.13 in population crossbred of female Java x male Kalimantan. While the lowest polymorphism and heterozygosity were detected on population of f Kalimantan x m Java (21.62% and 0.10, respectively). Based on the relationship between tinfoil barb hybrid (female Kalimantan x male Java) with a both broodstock population using three RAPD primers (OPA-08, OPA-09, and OPC-02) resulted in genetic distance of 0.48. Females tinfoil barb from Kalimantan and males from Java potential could increase genetic diversity.

PEMBENTUKAN POPULASI SINTETIS UNTUK PENINGKATAN KUALITAS GENETIK IKAN MAS Didik Ariyanto; Odang Carman; Dinar Tri Soelistyowati; Muhammad Zairin Junior; M. Syukur; Yogi Himawan; Flandrianto S. Palimirmo
Jurnal Riset Akuakultur Vol 16, No 2 (2021): (Juni, 2021)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Benih ikan mas telah mengalami penurunan kualitas genetik yang menyebabkan penurunan performa fenotipik di lingkungan budidaya. Salah satu upaya perbaikan genetik adalah melalui pembentukan populasi sintetis yang merupakan penggabungan potensi genetik beberapa populasi plasma nutfah ikan mas. Penelitian ini bertujuan membentuk dan mengevaluasi performa genotipik dan fenotipik populasi sintetis ikan mas, yang merupakan penggabungan dari strain Rajadanu, Majalaya, Sutisna, Wildan, dan Sinyonya. Performa genotipik dievaluasi menggunakan metode mikrosatelit DNA, sedangkan performa fenotipik dievaluasi menggunakan analisis biometrik terkait kegiatan budidaya. Hasil penelitian menunjukkan bahwa nilai keragaman genetik populasi sintetis lebih tinggi 55,0%-287,5% dengan tingkat inbreeding 40,0%-77,14% lebih rendah dibanding populasi-populasi pembentuknya. Hal ini berdampak terhadap performa fenotipik populasi sintetis yang lebih baik, diindikasikan dengan peningkatan panjang, bobot akhir, dan tingkat produktivitas, masing-masing sebesar 2,5%-20,6%; 9,4%-61,8%; dan 18,2%-66,0% lebih baik dibanding populasi-populasi pembentuknya. Peningkatan kualitas genetik dan performa fenotipik populasi sintetis ini memberikan peluang untuk memperbaiki kualitas benih ikan mas pada kegiatan budidaya.Common carp in Indonesia has experienced a decline in genetic quality. The progressive decline leads to a significant decrease in carp performance in the farming environment. One of the efforts to genetically improve carp growth performance is through developing synthetic carp populations, which is a blend of the genetic potentials from several germplasm populations. This study aimed to form and evaluate the performance of genotypic and phenotypic of synthetic populations of common carp, blended from five strains of common carp, i.e., Rajadanu, Majalaya, Sutisna, Wildan, and Sinyonya. The genotypic performance was evaluated using the DNA microsatellite method. The phenotypic performance was assessed using biometric analysis, especially in terms of culture performance. The results showed that the genotypic performance of the synthetic populations of common carp was better than that of the founder strains. This performance was indicated by higher genetic diversity values, about 55.0%-287.5% and lower levels of inbreeding, about 40.0%-77.1%, compared with their founder populations. Phenotypic performance of the synthetic populations is also better than their founder populations, indicated by higher body length, weight, and productivity, about 2.5%-20.6%, 9.4%-61.8%, and 18.2%-66.0%, respectively. The improvement on genetic quality and phenotypic performance of the synthetic population provide opportunities to improve the quality of common carp fry in aquaculture activity.

KARAKTERISTIK FENOTIPE DAN GENOTIPE LIMA STRAIN IKAN MAS DI JAWA BARAT DAN BANTEN Didik Ariyanto; Odang Carman; Dinar Tri Soelistyowati; Muhammad Zairin Jr.; Muhamad Syukur
Jurnal Riset Akuakultur Vol 13, No 2 (2018): (Juni, 2018)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Langkah awal program pemuliaan adalah koleksi dan pengenalan karakter materi pemuliaan tersebut. Hasil karakterisasi digunakan sebagai dasar pertimbangan metode pelaksanaan program pemuliaan yang akan dilakukan. Koleksi material genetik untuk program pemuliaan ikan mas menghasilkan lima strain yang dominan dibudidaya di wilayah Jawa Barat dan Banten, yakni strain Rajadanu, Sutisna, Majalaya, Wildan, dan Sinyonya. Pengenalan karakter material genetik ikan mas hasil koleksi dilakukan melalui dua pendekatan, yaitu fenotipe menggunakan metode truss morfometrik dan genotipe menggunakan metode mikrosatelit DNA. Hasil analisis menunjukkan bahwa variasi keragaan fenotipe kelima strain ikan mas relatif sesuai dengan variasi keragaan genotipenya. Selain mengelompokkan antar strain, hasil analisis genotipe juga menunjukkan bahwa tingkat keragaman genetik kelima strain ikan mas yang diindikasikan dengan nilai heterozigositas (Ho) relatif rendah, yaitu berkisar antara 0,08-0,20 dengan jarak genetik antar strain berada dalam kisaran 0,420-0,582.The first step in a fish breeding program is the collection and characterization of the breeding subject. The results of characterization are used as a baseline to select suitable potential methods used in the breeding program. The samples of genetic materials of five strains of common carp (Rajadanu, Sutisna, Majalaya, Wildan, and Sinyonya) were obtained from West Java and Banten Province. The characterization of collected genetic materials of the common carp species followed the phenotype and genotype approaches. Phenotypic characterization used truss morphometric method while genotype characterization applied DNA microsatellite method. The results showed that the phenotypic variation of the common carp had a close fit with its genotypic variation. In addition, the genotype analysis also showed that the genetic diversity level of the strains was relatively low indicated by the narrow ranges of heterozygosity values (Ho) (0.08-0.20) and genetic distance among strains (0.420-0.582).

KARAKTERISTIK GENETIK POPULASI TIRAM MUTIARA (Pinctada margaritifera) TERKAIT DENGAN DISTRIBUSI GEOGRAFISNYA DI PERAIRAN INDONESIA Rini Susilowati; Komar Sumantadinata; Dinar Tri Soelistyowati; Achmad Sudradjat
Jurnal Riset Akuakultur Vol 4, No 1 (2009): (April 2009)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Tujuan penelitian ini untuk memetakan keragaman genetik lima populasi tiram mutiara di Indonesia (Sumbawa, Bali Utara, Selat Sunda, Belitung, Sulawesi Selatan) dengan teknik mtDNA RFLP daerah amplifikasi Cytochrome Oxydase I (COI) dan hubungan kekerabatannya. Lima puluh tiram mutiara (Pinctada margaritifera) yang dianalisis menghasilkan DNA teramplifikasi sebesar 750 pb pada daerah COI mtDNA dengan teknik RFLP. Delapan belas komposit haplotipe terdeteksi dengan menggunakan tiga enzim restriksi: FokI, HaeIII, dan NlaIV. Diversitas haplotip rata-rata sebesar 0,255±0,093. Lima populasi tiram mutiara menghasilkan tiga kelompok dengan jarak genetik terendah adalah populasi Sumbawa dan Bali Utara (0,017) dan terjauh adalah populasi Sulawesi Selatan (0,142). Populasi Sulawesi Selatan merupakan populasi unik berdasarkan distribusi haplotipe BBCAA (60%) dengan nilai keragaman genetik terendah (0,105) dibandingkan dengan populasi lainnya (0,177-0,328).The objectives of this study were to map the genetic diversity of five populations of pearl oyster in Indonesian waters using restriction fragment length polymorphism analysis of DNA COI gene and their genetic relationships. A total of 50 individual of pearl oysters (Pinctada margaritifera) were analyzed for genetic variations within a 750-base pair region of the mitochondrial DNA COI gene using restriction fragment length polymorphism analysis. 18 composite haplotypes were detected following three digestions of endonuclease: FokI, HaeIII, and NlaIV. Five populations of pearl oysters formed three groups where the lowest values of Nei’s genetic distance were among Sumbawa and North Bali populations (0.017) and highest were among the South Sulawesi populations (0.142). The South Sulawesi populations possess uniqueness based on the haplotipe distribution of BBCAA (60%) with the lowest values of genetic diversities (0.105) compared to other populations (0.177--0.328).

PENYERAPAN KARBON PADA BUDIDAYA RUMPUT LAUT Kappaphycus alvarezii dan Gracilaria gigas DI PERAIRAN TELUK GERUPUK, LOMBOK TENGAH, NUSA TENGGARA BARAT Erlania Erlania; Kukuh Nirmala; Dinar Tri Soelistyowati
Jurnal Riset Akuakultur Vol 8, No 2 (2013): (Agustus 2013)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Pengikatan karbon oleh algae fotoautotrofik berpotensi untuk mengurangi pelepasan CO2 ke atmosfer dan dapat membantu mencegah percepatan terjadinya pemanasan global. Penelitian ini bertujuan untuk menganalisis tingkat penyerapan karbon pada budidaya rumput laut Kappaphycus alvarezii dan Gracilaria gigas berdasarkan aktivitas fotosintesis serta variabel-variabel yang mempengaruhinya. Budidaya rumput laut dilakukan dengan metode long-line di perairan Teluk Gerupuk, Lombok Tengah pada satu unit long-line dengan luas area 1.250 m2. Selama penelitian, dilakukan pengujian terhadap sampel rumput laut dan sampel air laut dari lokasi budidaya yang diambil pada hari ke-0, 10, 20, 30, dan 45 pemeliharaan. Laju penyerapan karbon total berdasarkan biomassa panen pada G. gigas hampir 300% lebih tinggi dibandingkan K. alvarezii. Selain itu, laju pertumbuhan dan produksi karbohidrat pada G. gigas juga lebih tinggi, yang mengindikasikan laju fotosintesis yang lebih tinggi, dan didukung oleh indeks percabangan yang juga lebih tinggi. Potensi penyerapan karbon di perairan Teluk Gerupuk mencapai 6.656,51 ton C/tahun untuk budidaya K. Alvarezii dan 19.339,02 ton C/tahun untuk budidaya G. gigas. Penyerapan karbon berhubungan dengan kandungan pigmen dan laju pertumbuhan rumput laut, serta konsentrasi CO2 dan kecerahan perairan.

KARAKTERISTIK GENETIK ENAM POPULASI IKAN NILEM (Osteochilus hasselti) DI JAWA BARAT Mulyasari Mulyasari; Dinar Tri Soelistyowati; Anang Hari Kristanto; Irin Iriana Kusmini
Jurnal Riset Akuakultur Vol 5, No 2 (2010): (Agustus 2010)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Penelitian karakteristik genetik populasi ikan nilem, Osteochilus hasselti di Jawa Barat dilakukan untuk mendapatkan data base sebagai langkah awal dalam melaksanakan program pemuliaan guna mempertahankan dan meningkatkan produksi dari ikan nilem di Jawa Barat. Tujuan penelitian ini adalah melakukan identifikasi genetik ikan nilem menggunakan metode RAPD dan menelusuri keragaman intra dan inter-populasi ikan nilem, Osteochilus hasselti di sentra budidaya yang terdapat di daerah Jawa Barat. Berdasarkan hasil penelitian, populasi Sumedang secara genetis memiliki keragaman paling tinggi dibandingkan dengan populasi lainnya serta alel spesifik yang tidak ditemukan pada populasi lain (1.100 bp). Sedangkan Sukabumi memiliki keragaman genetik dan jumlah alel yang paling rendah. Hubungan inter-populasi ikan nilem hijau di Jawa Barat tidak berbeda nyata di mana jarak genetik enam populasi ikan nilem tersebut berkisar antara 0,0153-0,1392.Research on genetic variation was done to conduct breeding program as the effort to maintain and increase the production of nilem carp fish at West Java. The aim of this study was to identify nilem carp genetically and to estimate the variation of the intra and inter population of nilem carp fish from West Java using RAPD methods. The result showed that Sumedang population had the highest genetic variation and had specific allele that cannot be found at other population (1,100 bp). But in contrast Sukabumi population had the lowest genetic variation and allele number. The inter- population relationship among fish from West Java were not significantly different. Genetic distance among population were between 0.0153-0.1392.

KERAGAAN GENOTIPE DAN FENOTIPE IKAN UCENG Nemacheilus fasciatus (Valenciennes, 1846) ASAL BOGOR, TEMANGGUNG, DAN BLITAR Muhammad Hunaina Fariduddin Ath-thar; Arifah Ambarwati; Dinar Tri Soelistyowati; Anang Hari Kristanto
Jurnal Riset Akuakultur Vol 13, No 1 (2018): (Maret 2018)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Ikan uceng (Nemacheilus fasciatus) merupakan ikan asli Indonesia yang hidup di sungai dan potensial sebagai komoditas budidaya lokal yang bernilai ekonomi. Pengenalan sumber genetik ikan uceng berdasarkan lokasi geografis perlu dilakukan untuk pengembangan budidaya jangka panjang. Penelitian ini bertujuan untuk mengevaluasi potensi genotipe dan fenotipe ikan uceng asal Bogor (Jawa Barat), Temanggung (Jawa Tengah), dan Blitar (Jawa Timur). Tiga primer (OPA-12, OPC-04, dan OPC-06) digunakan untuk analisis genotipe dengan metode PCR-RAPD, sedangkan performa fenotipik dievaluasi berdasarkan analisis truss morfometrik dan kinerja pertumbuhannya. Hasil penelitian menunjukkan bahwa ikan uceng asal Temanggung memiliki heterozigositas dan tingkat polimorfisme tertinggi yaitu 0,153 dan 34,69%. Fenotipe truss morfometrik interpopulasi ikan uceng asal Temanggung dan Bogor memiliki tingkat inklusivitas sebesar 10%, sedangkan populasi Blitar menunjukkan tingkat keseragaman intrapopulasi yang tertinggi (96,7%). Sintasan tertinggi terdapat pada populasi ikan uceng asal Temanggung (96,66 ± 3,33%) yang diikuti dengan peningkatan nilai faktor kondisi, namun laju pertumbuhan spesifik tertinggi yaitu populasi asal Blitar (1,082 ± 0,164%). Berdasarkan keragaan genotipe dan fenotipe populasi ikan uceng asal Temanggung menunjukkan potensial sebagai sumber genetik budidaya dengan tingkat keragaman genetik, sintasan, dan inklusivitas tertinggi.Barred loach (Nemacheilus fasciatus) is an Indonesian native fish and has a potential as an economically valuable aquaculture species. The genetics resource identification of barred loach based on geographical location is needed in order to determine its aquaculture potential. The purpose of this research was to evaluate the genotype and phenotype performance of barred loach originated from Bogor (West Java), Temanggung (Central Java), and Blitar (East Java). Three primer (OPA-12, OPC04, and OPC-06) were used in the genotype analysis using PCR-RAPD method, while phenotype performance was evaluated based on truss morphometric analysis and growth performance. The result indicated that barred loach from Temanggung had highest heterozigosity (0.153) and polymorphism (34.69%) compared to the others. The highest intrapopulation sharing component gained by barred loach from Blitar (96.7%), while the interpopulation sharing component by barred loach from Temanggung and Bogor (10%). Barred loach from Temanggung had the highest survival rate (96.66 ± 3.33%) with increasing of condition factor. The highest specific growth rate resulted by barred loach from Blitar (1.08 ± 0.16%). Barred loach from Temanggung potential for genetic resources as highest polymorphism, inclusivity, and survival rate.

Co-Authors , Alimuddin , Rahman, , Achmad Sudradjat Agus Oman Sudrajat Ahmad Fahrul Syarif Ahmad Muzaki Ahmad Teduh Akbar, Muhamad Saepul Aldilla Kusumawardhani, Aldilla Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Anang Hari Kristanto Anang Hari Kristanto Aras, Annisa Khairani Arifah Ambarwati Asep Bulkini Bagus Rahmat Basuki Daniel Djokosetiyanto Dendi Hidayatullah, Dendi Deni Radona Deni Radona Dian Hardiantho Dian Hardianto Didik Ariyanto Didik Ariyanto Donny Prariska Eddy Supriyono Edison, Thomas Erlania Erlania Euis Rakhmawati Fajar Maulana . Farah Diana Fauzan, Agung Luthfi Firmansyah, Rodhi Fitriyah Husnul Khotimah Flandrianto S. Palimirmo Gleni Hasan Huwoyon Gleni Hasan Huwoyon Gleni Hasan Huwoyon, Gleni Hasan Gloria Ika Satriani Gloria Ika Satriani Gunawan, Bambang Kusmayadi Gusti Ngurah Permana Guttifera Hafidah, Riva Hafif Syahputra Hanggara, Yudha Harton Arfah Haryanti Haryanti Haryanti Haryanti Helena Sahusilawane Herjayanto, Muh. Ibrahim Satrio Faqih Ibrahim, Yusran Ida Ayu Amarilia Dewi Murni Imron Imron, Imron Indah Mastuti Intan Putriana Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini, Irin Iriana Irwan Irwan Irzal Effendi Iskandariah Iskandariah Iskandariah, Iskandariah Ketut Mahardika Ketut Sugama Ketut Sugama Khotimah, Fitriyah Husnul Komar Sumantadinata Kukuh Nirmala Ligaya I. T. A. Tumbelaka M. H. Fariduddin Ath-thar M. Syukur M. Zairin Junior Mahardhika, Prana Mahdaliana, Mahdaliana Maulana, Fajar Melta Rini Fahmi MH. Fariduddin Ath-thar Mia Setiawati Muh. Herjayanto Muhamad Syukur Muhammad Fadlan Furqon Muhammad Hunaina Fariduddin Ath-thar Muhammad Sami Daryanto Muhammad Zairin Jr Muhammad Zairin Jr. Muhmmad Agus Suprayudi Muliari Muliari Mulyasari Mulyasari Nopri Yanto Odang Carman Oktaviani, Tia Peni Pitriani Poppy Dea Bertha, Poppy Dea Prana Mahardhika Prassetyo Dwi Dhany Wijaya Rahmadani Rahmadani Ratu Siti Aliah Raudhatus Sa'adah Rezki, Dinda Wahyu RIDWAN AFFANDI Rinaldi Rinaldi Rini Susilowati Rizki Eka Puteri Ronny Rachman Noor Ruby Vidia Kusumah Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Safira Qisthina Ayuningtyas, Safira Qisthina Sahusilawane, Helena Afia Sandra, Aan Aryanti Sari Budi Moria Sembiring Sari Budi Moria Sembiring Satriani, Gloria I. Selly Ratna Sari Sholihin, Hidayatush Sri Nuryati Sri Sundari Sudarto Sudarto Syahril, Alfis Tia Oktaviani Upmal Deswira Vitas Atmadi Prakoso Wahyutomo Wahyutomo Wahyutomo, Wahyutomo Wibowo, Kesit Tisna Wicaksono, Aryo Wenang WIDANARNI WIDANARNI Wildan Nurussalam Wiyoto Wiyoto Yeni Elisdiana Yogi Himawan

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