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All Journal Jurnal Akuakultur Indonesia Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia BERITA BIOLOGI Jurnal Akuatika Indonesia Omni-Akuatika Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Perikanan Universitas Gadjah Mada Sains Natural: Journal of Biology and Chemistry Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Tolis Ilmiah: Jurnal Penelitian Clarias : Jurnal Perikanan Air Tawar Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine Jurnal Perikanan
Dinar Tri Soelistyowati
Department Of Aquaculture, Faculty Of Fisheries And Marine Sciences, IPB University Bogor, West Java, 16680. Indonesia
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Identification of Kappaphycus alvarezii seaweed based on phylogenetic and carrageenan conten Satriani, Gloria Ika; Tri Soelistyowati, Dinar; Alimuddin; Arfah, Harton; Effendi, Irzal
Jurnal Akuakultur Indonesia Vol. 23 No. 1 (2024): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.23.1.1-11

Abstract

Increasing seaweed production requires accurate information regarding the genetic sources of seeds used. Identifying the seaweed species Kappaphycus molecular is one of the solutions to ensure seaweed cultivators choose seeds for their cultivation businesses. Molecular identification is essential for the system traceability of seaweed products and the creation of databases regarding species variant information Kappaphycus alvarezii cultivation as potential data collection for developing and genetically breeding seaweed seeds. To date, there is no information on the genetic potential of K. alvarezii cultivated in various seaweed cultivation centers in Indonesia. This study aimed to obtain phylogenetic details based on identification of the genetic source using DNA molecular markers barcoding rbcL and analysis of carrageenan content using the Fourier transform infra-red (FTIR) spectrum. The results of DNA sequencing analysis and FTIR testing of 16 varieties of seaweed seedlings obtained from various cultivation centers in Indonesia showed 99% similarity with K. alvarezii, a producer of kappa carrageenan. Keywords: DNA sequencing, phylogenetics, haplotypes, kappa-carrageenan, rbcL ABSTRAK Peningkatan produksi rumput laut memerlukan informasi yang akurat mengenai kepastian sumber genetik bibit yang digunakan. Identifikasi spesies rumput laut Kappaphycus secara molekuler merupakan salah satu solusi untuk memberikan kepastian pada pembudidaya rumput laut untuk memilih bibit bagi usaha budidaya. Identifikasi molekuler sangat penting dalam sistem traceability produk rumput laut dan pembuatan basis data mengenai informasi varian spesies Kappaphycus alvarezii budidaya sebagai pendataan potensi untuk pengembangan dan pemuliaan bibit rumput laut secara genetis. Sampai saat ini belum tersedia informasi mengenai potensi genetik rumput laut K. alvarezii yang dibudidayakan di berbagai sentra budidaya rumput laut di Indonesia. Penelitian ini bertujuan untuk memperoleh informasi filogenetik berdasarkan identifikasi sumber genetiknya menggunakan penanda molekuler DNA barcoding rbcL serta analisis kandungan karaginannya menggunakan spektrum Fourier Transform Infra-Red (FTIR). Dari hasil analisis sekuensing DNA dan pengujian FTIR terhadap 16 varietas bibit rumput laut yang diperoleh di berbagai sentra budidaya di Indonesia menghasilkan 99% kemiripan yang tinggi dengan K. alvarezii penghasil kappa karagenan. Kata kunci: DNA sekuensing, filogenetik, haplotipe, kappa-karagenan, rbcL
Sex determination and acclimation response of dwarf snakehead fish Channa limbata from West Java Tri Soelistyowati, Dinar; Oman Sudrajat, Agus; Arfah, Harton; Alimuddin, Alimuddin; Hafidah, Riva; Hanggara, Yudha; Edison, Thomas
Jurnal Akuakultur Indonesia Vol. 23 No. 2 (2024): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.23.2.201-211

Abstract

The Channa limbata fish is a type of tropical freshwater fish of the Channidae family which is relatively small as an aquarium ornamental fish with a distinctive color at the tip of its dorsal fin and has a snake-like head (dwarf snakehead). Natural snakehead fishing activities have threatened its sustainability. Breeding C. limbata fish through cultivation can increase its potential for sustainable use. This study aims to evaluate the acclimation response of wild-type dwarf snakehead fish in captivity and its sexual characteristics as a basis for domestication and hatchery technology. The fish samples used were natural catches from rivers in West Java measuring <100 mm to >150 mm of body length then individually acclimated indoors in an aquarium (35×20×20 cm) for 14 days. Snakehead fish live in shallow, slow-flowing river waters with a temperature of 20.2-21.3°C, TDS 16-24 mg/L at neutral pH, while the rearing water temperature and TDS are higher (temperature: 24.9-27.6°C; TDS: 88-110 mg/L). The fish mortality rate during acclimation reached 25% in fish measuring >150 mm of length on tenth day, while fish measuring <150 mm more adaptive with 100% survival. The male fish measuring 100-150 mm have 13-15 pectoral fin rays while female fish have fewer (13-14). The gonad development level of male C. limbata in nature is slower than female fish measuring 100-150 mm with a gonadosomatic index of ovaries reached 10 times higher than testicular. Keywords: acclimation, C. limbata, gonadosomatic index, ovaries ABSTRAK Ikan Channa limbata merupakan jenis ikan air tawar tropis dari famili Channidae yang berukuran relatif kecil sebagai ikan hias akuarium dengan warna yang khas pada ujung sirip punggungnya dan bentuk kepala mirip ular (dwarf snakehead). Aktivitas penangkapan ikan gabus alam telah mengancam kelestariannya. Pembibitan ikan C.limbata melalui budidaya dapat meningkatkan potensi pemanfaatannya secara berkelanjutan. Penelitian ini bertujuan untuk mengevaluasi respons aklimatisasi ikan gabus alam di dalam penangkaran dan karakterisasi seksualnya sebagai landasan teknologi pembenihan ikan gabus C. limbata yang tepat. Sampel ikan yang digunakan merupakan hasil tangkapan alam dari sungai di Jawa Barat berukuran <100 mm hingga >150 mm kemudian diaklimasi indoor di akuarium (35×20×20 cm) selama 14 hari. Ikan gabus hidup di perairan sungai yang dangkal berarus lambat dengan suhu 20,2-21,3°C, TDS 16-24 mg/L dan pH netral, sedangkan suhu air pemeliharaan dan TDS lebih tinggi (suhu: 24,9-27,6°C; TDS: 88-110 mg/L). Angka kematian ikan selama aklimatisasi mencapai 25% pada ikan berukuran >150 mm hari ke 10, sedangkan ikan berukuran <150 mm lebih adaptif dengan sintasan 100%. Ikan jantan C.limbata berukuran 100-150 mm memiliki jari-jari sirip pektoral berjumlah 13-15, sedangkan ikan betina lebih sedikit (13-14). Tingkat perkembangan gonad ikan jantan lebih lambat dari pada ikan betina dengan indeks gonadosomatik ovarium mencapai 10 kali lipat lebih tinggi dibandingkan testis. Kata kunci: aklimatisasi, Channa limbata, indeks gonadosomatik, ovarium
Induction of reproduction of fish Anasa Nomorhampus sp. endemic Palu, Central Sulawesi orally through hormon bioencapsulation use Chironomus sp. Rezki, Dinda Wahyu; Sudrajat, Agus Oman; Soelistyowati, Dinar Tri; Carman, Odang
Jurnal Akuakultur Indonesia Vol. 24 No. 2 (2025): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.24.2.220-232

Abstract

Species Nomorhampus sp. with the local name Anasa fish, endemic to Palu, Central Sulawesi, has a unique superior mouth shape, beak-shaped jaws, attractive colors and patterns, becoming an export commodity with high economic value, but currently it cannot be cultivated, domestication efforts are needed to avoid extinction, by carrying out hormonal manipulation that accelerates the domestication process. This study aims to evaluate the effectiveness of Oodev® on the induction of reproduction in the fish Nomorhampus sp. through bioencapsulation using Chironomus sp. which resulted in births, five groups of fish were fed using Oodev® at a dose of 1 mL/kg parent weight and NaCl 1 mL/kg parent weight as a control group, the fish were kept for 60 days. This research focuses on assessing specific weight growth rate (LPBS), specific length growth rate (LPPS), survival rate (TKH), gonadosomatic index (IGS), gonadal histology, birth frequency, number of births, and growth performance. Fish fed Oodev® feed showed higher SGR and IGS (p<0.05), 40% of fish fed Oodev® supplementary feed successfully gave birth with a total of 9 fry, while no birth occurred in control fish. Histological analysis showed faster gonad development in fish fed Oodev®. Hormonal induction with Oodev® can accelerate reproduction in anasa fish in cultivation containers. These findings provide valuable insight for fish farmers regarding the effect of Oodev® on gonad development in anasa fish in both male and female parents. It is hoped that this discovery will speed up the process of domestication of Anasa fish. Keywords: domestication, endemic, Nomorhampus sp., Oodev®, reproduction ABSTRAK Spesies Nomorhampus sp. dengan nama lokal ikan Anasa endemik Palu, Sulawesi Tengah, memiliki keunikan bentuk mulut superior rahang berbentuk paruh, warna dan corak menarik menjadi komoditas ekspor dengan nilai ekonomis yang tinggi, namun saat ini belum dapat dibudidayakan, perlu upaya domestikasi agar tidak terjadi kepunahan, dengan melakukan manipulasi hormormonal yang mempercepat proses domestikasi. Penelitian ini bertujuan untuk mengevaluasi efektivitas Oodev® terhadap induksi reproduksi ikan Nomorhampus sp. melalui bioenkapsulasi menggunakan Chironomus sp. yang menghasilkan kelahiran, lima kelompok ikan yang diberi pakan menggunakan Oodev® dosis 1 mL/kg bobot induk dan NaCl 1 mL/kg bobot induk sebagai kelompok kontrol, ikan dipelihara selama 60 hari. Penelitian ini fokus pada penilaian laju pertumbuhan bobot spesifik (LPBS), laju pertumbuhan panjang spesifik (LPPS), tingkat kelangsungan hidup (TKH), indeks gonadosomatik (IGS), histologi gonad, frekuensi kelahiran, jumlah kelahiran, dan kinerja pertumbuhan. Ikan yang diberi pakan Oodev® menunjukkan SGR dan IGS yang lebih tinggi (p<0,05), 40% ikan yang diberi pakan tambahan Oodev® berhasil melahirkan dengan jumlah total 9 ekor benih, sementara pada ikan kontrol tidak terjadi kelahiran. Analisa histologi menunjukkan perkembangan gonad yang berkembang lebih cepat pada ikan yang diberi pakan Oodev®. Induksi hormonal dengan Oodev® mampu mempercepat reproduksi pada ikan anasa di wadah budidaya. Temuan ini memberikan wawasan berharga bagi pembudidaya ikan mengenai pengaruh Oodev® terhadap perkembangan gonad pada ikan anasa baik pada induk jantan maupun betina. Penemuan ini diharapkan dapat mempercepat proses domestikasi ikan Anasa. Kata kunci: domestikasi, endemik, Nomorhampus sp., Oodev®, reproduksi
Induction of maturation gonads the Asian swamp eel Monopterus albus at different sizes with the hormones PMSG + antidopamine and hCG Sandra, Aan Aryanti; Sudrajat, Agus Oman; Arfah, Harton; Soelistyowati, Dinar Tri
Jurnal Akuakultur Indonesia Vol. 24 No. 2 (2025): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.24.2.274-287

Abstract

Monopterus albus is a protogynous hermaphrodite species that undergoes a unidirectional sex change from female to male. Asian swamp eel cultivation activities face constraints in seeding due to limited mature gonad broodstock. This study aims to evaluate the effect of administering a combination of PMSG + Antidopamine (OodevⓇ) and hCG on the induction of gonad maturation of Asian swamp eels at various body sizes. The research design used was a factorial design consisting of control treatment (without hormone injection), P1 = hCG (dose 20 IU/kg), P2 = OodevⓇ (dose 0.5 ml/kg), and P3 = hCG+OodevⓇ (dose 20 IU+0.5 ml/kg) with body length groups, namely K1 (15–25 cm), K2 (30–40 cm), and K3 (45–55 cm). The results showed that eels in group K1 had the highest body length growth (3.63 ± 0.96 cm), while the highest body weight gain (5.30 ± 1.30 grams) in K3 (P<0.05). Administration of oodevⓇ showed the highest values ​​for GSI = 8.13 ± 0.72% and HSI = 4.36±0.80% in K1 compared to K2 (GSI = 6.95 ± 2.86%; HSI = 3.26 ± 0.68%) with female sex. In contrast, group K3 experienced a decrease in GSI = 0.81 ± 0.11% and HSI = 2.48 ± 1.06% accompanied by masculinization. Administration of oodevⓇ also increased the concentration of estradiol-17β in K1 and K2, while testosterone increased in K3. The conclusion of this study is that administering oodevⓇ to 15-40 cm sized Asian swamp eels can accelerate gonad maturity with female sex status, while at sizes >45 cm it can accelerate masculinization and maturation of male gonads. Keywords: body length size, gonad maturation, masculinization, Monopterus albus, oodevⓇ ABSTRAK Monopterus albus adalah spesies hermaprodit protogini yang mengalami perubahan jenis kelamin dari betina ke jantan secara searah. Kegiatan budidaya belut sawah menghadapi kendala dalam pembenihan karena keterbatasan induk matang gonad. Penelitian ini bertujuan untuk mengevaluasi pengaruh pemberian kombinasi PMSG + Antidopamine (OodevⓇ) dan hCG terhadap induksi pematangan gonad belut sawah pada berbagai ukuran tubuh. Desain penelitian yang digunakan adalah rancangan faktorial yang terdiri dari perlakuan kontrol (tanpa injeksi hormon), P1 = hCG (dosis 20 IU/kg), P2 = OodevⓇ (dosis 0,5 ml/kg), dan P3 = hCG+OodevⓇ (dosis 20 IU+0,5 ml/kg) dengan kelompok ukuran panjang tubuh yaitu K1 (15–25 cm), K2 (30–40 cm), dan K3 (45–55 cm). Hasil penelitian menunjukkan bahwa belut kelompok K1 memiliki pertumbuhan panjang tubuh tertinggi (3,63 ± 0,96 cm), sementara pertambahan bobot tubuh tertinggi (5,30±1,30 gram) pada K3 (P<0,05). Pemberian oodevⓇ menunjukkan nilai tertinggi untuk GSI = 8,13±0,72% dan HSI = 4,36±0,80% pada K1 dibandingkan dengan K2 (GSI = 6,95±2,86%; HSI = 3,26±0,68%) dengan jenis kelamin betina. Sebaliknya, kelompok K3 mengalami penurunan nilai GSI = 0,81±0,11% dan HSI = 2,48±1,06% disertai dengan maskulinisasi. Pemberian oodevⓇ juga meningkatkan konsentrasi estradiol-17β pada K1 dan K2, sedangkan testosterone meningkat pada K3. Kesimpulan penelitian ini adalah pemberian oodevⓇ pada belut sawah ukuran 15-40 cm dapat mempercepat kematangan gonad dengan status kelamin betina sedangkan pada ukuran >45 cm dapat mempercepat maskulinisasi dan pematangan gonad jantan. Kata kunci: maskulinisasi, Monopterus albus, oodevⓇ, pematangan gonad, ukuran panjang tubuh
The Genetic Relationship Analysis of Genus Nomorhamphus from Lindu Lake, Central Sulawesi and Adaptive Responses to Exposure Different Light Wavelengths Hafidah, Riva; Soelistyowati, Dinar Tri; Sudrajat, Agus Oman; Alimuddin
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 2 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i2.55837

Abstract

Graphical Abstract Highlight Research Genus Nomorhamphusfrom three river inlets of Lindu Lake, Central Sulawesi was identified as Nomorhamphus with CO1 gene. Green wavelength can accelerate growth and maturation. Wild fish Nomorhamphus can complete life cycles in an aquaculture environment. Domestication level two is completed. Abstract Genus Nomorhamphus is an endemic fish that can be found in Sulawesi, Indonesia. This fish belongs to the halfbeak group and has diverse colors and morphology. It has economic value as an export commodity in the ornamental fish trade. Exploration of the genetic relationship of genus Nomorhamphus in Central Sulawesi is still limited. Nomorhamphus's fulfillment of export demand still comes from wild catches. This study aimed to identify the genetic relationship of genus Nomorhamphus in the inlet rivers of Lake Lindu, Central Sulawesi, and evaluate the adaptation response of genus Nomorhamphus to different wavelengths of light exposure. Caudal fin of six fish from the three inlet rivers of Lake Lindu were preserved in 95% ethanol for DNA extraction purposes. This research used a completely randomized design with three treatment, namely rearing under white (400 nm), green (525 nm), and red (625 nm) light for 60 days. Each treatment had three replications in the form of aquariums, with each aquarium being filled with four fish. A total of 42 fish were utilized in the study. CO1 sequence was amplified with universal primers of FISH-F2 and FISH-R2. The PCR amplification products were then sequenced and performed with phylogenetic tree analysis. The genetic diversity analysis suggests that the genus Nomorhamphus of all three rivers, Lindu Lake, Central Sulawesi is one species as Nomorhamphus sp.. Male and female growth length and gonad maturation were developed faster under green light exposure, while survival rate, blood glucose level, and color quality were not significantly different under different light. Exposure of wild fish to green light Nomorhamphus sp. can accelerate gonad maturation and growth to accelerate domestication.
Selection and cultivation of seaweed Kappaphycus alvarezii using a long line system (outdoor) Satriani, Gloria I.; Soelistyowati, Dinar Tri; Alimuddin, Alimuddin; Arfah, Harton; Effendi, Irzal
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 12, No 3 (2023): DECEMBER 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.3.30211

Abstract

In outdoor cultivation at Amal Beach, three seeding mass selections were reared for 30 days for each cycle. Each variety had a different DGR %/day (Daily Growth Rate %/day) value at the end of the growing season on G1-G2-G3 ranging from 3.62-5.90 %/day, which were still categorized as good, and G3 var.Lampung had the highest DGR value. Carrageenan yield values in this study, G1 to G3, ranged from 23.16% to 45.87%, where the G3 G2 G1 treatment. The best thallus samples (20% were selected for each generation) resulted in carrageenan yield sample population in each variety, where var.Lampung var.Tarakan var.Kupang. The biplot analysis shows that the DGR variable vector coincides with the IAA, Phosphate, Gibberellin, and Ammonia vectors. Ammonia stepwise regression analysis (sig) affects DGR with an Ammonia coefficient value of 4.89; meaning that for each increase in the Ammonia value by one unit, the estimated mean value of DGR will increase by 4.89. Thus, the application of themassbased on the results of this study was able to increase the DGR valueKappaphycus alvarezii,which in turn produce the highest yield of carrageenan withK. alvareziivar.Lampung G3 was the best seed candidate to be developed at the seaweed cultivation site in Tarakan City.Keywords:AlgaePhytohormonesCarrageenanNitrogenViscosity
Selection and cultivation of seaweed Kappaphycus alvarezii using a long line system (outdoor) Satriani, Gloria I.; Soelistyowati, Dinar Tri; Alimuddin, Alimuddin; Arfah, Harton; Effendi, Irzal
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 12, No 3 (2023): DECEMBER 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.3.30211

Abstract

In outdoor cultivation at Amal Beach, three seeding mass selections were reared for 30 days for each cycle. Each variety had a different DGR %/day (Daily Growth Rate %/day) value at the end of the growing season on G1-G2-G3 ranging from 3.62-5.90 %/day, which were still categorized as good, and G3 var.Lampung had the highest DGR value. Carrageenan yield values in this study, G1 to G3, ranged from 23.16% to 45.87%, where the G3 G2 G1 treatment. The best thallus samples (20% were selected for each generation) resulted in carrageenan yield sample population in each variety, where var.Lampung var.Tarakan var.Kupang. The biplot analysis shows that the DGR variable vector coincides with the IAA, Phosphate, Gibberellin, and Ammonia vectors. Ammonia stepwise regression analysis (sig) affects DGR with an Ammonia coefficient value of 4.89; meaning that for each increase in the Ammonia value by one unit, the estimated mean value of DGR will increase by 4.89. Thus, the application of themassbased on the results of this study was able to increase the DGR valueKappaphycus alvarezii,which in turn produce the highest yield of carrageenan withK. alvareziivar.Lampung G3 was the best seed candidate to be developed at the seaweed cultivation site in Tarakan City.Keywords:AlgaePhytohormonesCarrageenanNitrogenViscosity
POLA PERTUMBUHAN DAN VARIASI GENETIK BERBASIS DNA MIKROSATELIT DARI TIGA POPULASI IKAN BARAMUNDI Lates calcarifer Khotimah, Fitriyah Husnul; Alimuddin, Alimuddin; Soelistyowati, Dinar Tri; Nuryati, Sri; Arfah, Harton; Sugama, Ketut; Permana, Gusti Ngurah; Sembiring, Sari Budi Moria; Haryanti, Haryanti
Jurnal Riset Akuakultur Vol 18, No 1 (2023): (Maret 2023)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.18.1.2023.49-59

Abstract

Benih ikan baramundi Lates calcarifer diperoleh dari pemijahan alami dengan jumlah induk terbatas sehingga variabilitas pertumbuhan dan kelangsungan hidup antar-batch menjadi tinggi. Penelitian dilakukan untuk menganalisis pertumbuhan dan mengevaluasi variasi genetik ikan kakap putih populasi Australia, Situbondo dan Lampung hasil domestikasi dan dibudidaya di hatcheri skala rumah tangga (HSRT). Sebanyak 10 ekor ikan barramundi dari setiap populasi digunakan untuk analisis variabilitas genetik dengan dua lokus mikrosatelit, yaitu Lca21 dan Lca32. Selanjutnya, data mikrosatelit diolah menggunakan software genetic analysis in excel (GenAlEx 6.51b2). Hasil yang diperoleh menunjukkan bahwa pertumbuhan panjang dan bobot tubuh serta laju pertumbuhan spesifik ikan baramundi dari Australia lebih tinggi dibandingkan dari Situbondo dan Lampung (P<0,05); sedangkan ikan baramundi dari Situbondo dengan Lampung adalah sama (P>0,05). Jumlah alel setiap lokus ikan baramundi berkisar 2-8 alel dan heterozigositas tertinggi dimiliki oleh ikan barramundi asal Situbondo (0,85), diikuti Lampung (0,65) dan paling rendah dari Australia (0,54). Dari hasil riset tersebut dapat disimpulkan bahwa ketiga populasi ikan baramundi hasil domestikasi dan dipeliharan dalam sistem HSRT memenuhi kelayakan untuk digunakan untuk kegiatan hibridisasi atau membentuk populasi awal (sintetis). Barramundi seeds, Lates calcarifer are usually sourced from natural spawning using a limited number of broodstock. Therefore, the growth and survival rate of these seeds vary greatly between batches. The research was performed to determine the growth pattern and genetic variations of barramundi seed populations produced from domesticated broodstock sourced from Australia, Situbondo, and Lampung and reared in small-scale hatcheries. Ten individuals of barramundi from each population were used for microsatellite analysis using two microsatellite loci, namely: Lca 21 and Lca 32. The resulted microsatellite data was processed using the genetic analysis available in Excel software (GenAlEx 6.51b2). The results showed that the growth in length and body weight as well as the specific growth rate of barramundi seeds produced from Australia broodstock were higher than that of Situbondo and Lampung (P<0.05) while the later two were similar (P>0.05). The number of microsatellite alleles ranged from 2-8 and the highest heterozygosity was obtained by barramundi seeds produced by Situbondo (0.85), followed by Lampung (0.65), dan Australia (0.54) broodstock. From the results of the research, it can be concluded that the three populations of barramundi fish, which were domesticated and reared in the HSRT system, meet the criteria for use in hybridization program or for forming a synthetic population.
INDUKSI HORMONAL PADA SEX REVERSAL UDANG GALAH (Macrobrachium rosenbergii) MENGGUNAKAN EKSTRAK SERBUK SARI PINUS (Pinus tabulaeformis) Arfah, Harton; Soelistyowati, Dinar Tri; Sholihin, Hidayatush; Wibowo, Kesit Tisna; Sudrajat, Agus Oman; Carman, Odang; Maulana, Fajar; Alimuddin, Alimuddin
Jurnal Riset Akuakultur Vol 19, No 2 (2024): Juni (2024)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.19.2.2024.85-95

Abstract

Udang galah (Macrobrachium rosenbergii) merupakan komoditas perikanan air tawar yang memiliki ciri dimorfisme seksual pada pertumbuhan individu jantan lebih cepat dibanding betina. Pembentukan populasi monoseks jantan potensial dalam budidaya bertujuan untuk meningkatkan keseragaman, memacu pertumbuhan, dan efisiensi produksi. Induksi sex reversal pada pembentukan individu neomale dapat dilakukan secara hormonal menggunakan bahan yang mengandung derivat testosteron, yaitu serbuk sari pinus (Pinus tabulaeformis). Penelitian ini bertujuan untuk mengevaluasi efektivitas ekstrak serbuk sari pinus terhadap performa sex reversal pada udang galah. Ekstrak serbuk sari pinus dengan dosis berbeda (0,0 mL L-1; 0,2 mL L-1; 0,4 mL L-1; dan 0,6 mL L-1) diberikan melalui perendaman selama 24 jam pada larva udang galah sebelum berdiferensiasi kelamin (PL15) berukuran panjang 12 ± 0,3 mm dan bobot 0,02 ± 0,001 g dengan kepadatan 60 individu per L, kemudian dipelihara selama 45 hari dalam kontainer (60 x 40 x 30 cm3) dengan tinggi air 20 cm dan kepadatan 1 individu per L. Perlakuan dosis ekstrak serbuk sari pinus dosis 0,2 mL L-1 menghasilkan nisbah kelamin jantan 46,69 ± 5,36%, sedangkan peningkatan dosis 0,4-0,6 mL L-1 dan kontrol tidak efektif mengarahkan kelamin jantan (35-42%) dengan tingkat kelangsungan hidup udang PL60 berkisar 66-82%. Ciri kelamin sekunder udang galah jantan PL60 berukuran 30-32 mm berupa male gonopore (MG) dan appendix masculine (AM), sedangkan betina memiliki appendix interna (AI) pada kaki renang kedua dan rongga di antara kedua kaki jalan kelima. Aplikasi ekstrak serbuk sari pinus pada dosis maksimal 0,2 mL L-1 dapat mengarahkan kelamin jantan udang galah.Giant freshwater prawn (Macrobrachium rosenbergii) is a freshwater fishery commodity with sexual dimorphism in which male individuals grow faster than females. The establishment of a male monosexual population is expected to increase individual uniformity, growth rate, and production efficiency. Induction of sex reversal in the formation of the pseudomale can be done hormonally using materials containing testosterone derivatives, for example, pine pollen (Pinus tabulaeformis). This study aimed to evaluate the effectiveness of pine pollen extract on the sex reversal performance of giant prawns. Pine pollen extract with different doses (0.0 mL L-1; 0.2 mL L-1; 0.4 mL L-1; and 0.6 mL L-1) was given by immersion during 24 hours in prawns before sexual differentiation (PL15) with a length of 12 ± 0.3 mm and a weight of 0.02 ± 0.001 g with a density of 60 individuals per L, then reared for 45 days in a container (60 x 40 x 30 cm3) with a water height of 20 cm and a density of 1 individual per L. Treatment with a dose of pine pollen extract at a dose of 0.2 mL L-1 resulted in a male sex ratio of 46.69 ± 5.36%, while increasing doses of 0.4-0.6 ml L-1 and the control were not effective in the sex reversal (35-42%) with a survival rate of PL60 ranged from 66-82%. The secondary sexual characteristics of PL60 male giant prawns measuring 30-32 mm are male gonopore (MG) and masculine appendix (AM), while female giant prawns have an internal appendix (AI) on the second swimming leg and the cavity between the fifth swimming leg. This study concludes that the application of pine pollen extract at a maximum dose of 0.2 mL L-1 could directly induced sex reversal of female to male of giant prawns.
Molecular, Morphological and Production Performance Analysis of Introduced Black Tilapia Strains Oreochromis sp. Yanti, Dwi Hany; Soelistyowati, Dinar Tri; Carman, Odang; Hardiantho, Dian; Nasrullah, Hasan; Alimuddin, Alimuddin
Jurnal Perikanan Universitas Gadjah Mada Vol 27, No 2 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.103567

Abstract

Evaluating the introduced black tilapia strains from molecular to production performance is essential in aquaculture to ensure genetic purity, reproductive efficiency and sustainable production improvement. However, uncontrolled hybridization and limited molecular evaluation of introduced strains have often led to inconsistent performance in hatcheries and grow-out systems. This study aimed to identify and characterize introduced black tilapia strains from Thailand (MAG NIN, BIG NIN and GIFT) compared to locally developed SAKTI strain from Indonesia based on molecular, morphological and production performance aspects. Molecular characterization was performed using cytochrome oxidase subunit I (COI) gene sequences from three fish per strain. Morphological aspects were assessed based on body dimensions and proportions from ten fish per strain. Production performance focused on reproductive and growth parameters. Reproductive parameters  including fecundity, egg size, hatching rate and larval survival rate, while growth performance parameters comprised specific growth rate, aquaculture productivity, feed conversion ratio, sex ratio and survival rate. Results revealed that all introduced strains shared the same species as the SAKTI strain, identified as Oreochromis niloticus. Morphologically, BIG NIN exhibited a significantly longer body shape compared to other strains (p < 0.05). BIG NIN also demonstrated superior production performance (p < 0.05). These findings highlight the importance of integrating molecular and performance-based evaluations to support selective breeding and strain improvement  programs for enhancing tilapia productivity.
Co-Authors , Alimuddin , Rahman, , Achmad Sudradjat Agus Oman Sudrajat Ahmad Fahrul Syarif Ahmad Muzaki Ahmad Teduh Akbar, Muhamad Saepul Aldilla Kusumawardhani, Aldilla Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Anang Hari Kristanto Anang Hari Kristanto Aras, Annisa Khairani Arifah Ambarwati Asep Bulkini Bagus Rahmat Basuki Daniel Djokosetiyanto Dendi Hidayatullah, Dendi Deni Radona Deni Radona Dian Hardiantho Dian Hardianto Didik Ariyanto Didik Ariyanto Donny Prariska Dwi Hany Yanti Eddy Supriyono Edison, Thomas Erlania Erlania Euis Rakhmawati Fajar Maulana . Farah Diana Fauzan, Agung Luthfi Firmansyah, Rodhi Fitriyah Husnul Khotimah Flandrianto S. Palimirmo Gleni Hasan Huwoyon Gleni Hasan Huwoyon Gleni Hasan Huwoyon, Gleni Hasan Gloria Ika Satriani Gloria Ika Satriani Gunawan, Bambang Kusmayadi Gusti Ngurah Permana Guttifera Hafidah, Riva Hafif Syahputra Hanggara, Yudha Harton Arfah Haryanti Haryanti Haryanti Haryanti Helena Sahusilawane Herjayanto, Muh. Ibrahim Satrio Faqih Ibrahim, Yusran Ida Ayu Amarilia Dewi Murni Imron Imron, Imron Indah Mastuti Intan Putriana Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini, Irin Iriana Irwan Irwan Irzal Effendi Iskandariah Iskandariah Iskandariah, Iskandariah Ketut Mahardika Ketut Sugama Ketut Sugama Khotimah, Fitriyah Husnul Komar Sumantadinata Kukuh Nirmala Ligaya I. T. A. Tumbelaka M. H. Fariduddin Ath-thar M. Syukur M. Zairin Junior Mahardhika, Prana Mahdaliana, Mahdaliana Maulana, Fajar Melta Rini Fahmi MH. Fariduddin Ath-thar Mia Setiawati Muh. Herjayanto Muhamad Syukur Muhammad Fadlan Furqon Muhammad Hunaina Fariduddin Ath-thar Muhammad Sami Daryanto Muhammad Zairin Jr Muhammad Zairin Jr. Muhmmad Agus Suprayudi Muliari Muliari Mulyasari Mulyasari Nasrullah, Hasan Nopri Yanto Odang Carman Oktaviani, Tia Peni Pitriani Poppy Dea Bertha, Poppy Dea Prana Mahardhika Prassetyo Dwi Dhany Wijaya Rahmadani Rahmadani Rahmi, Kurnia Anggraini Ratu Siti Aliah Raudhatus Sa'adah Rezki, Dinda Wahyu RIDWAN AFFANDI Rinaldi Rinaldi Rini Susilowati Rizki Eka Puteri Ronny Rachman Noor Ruby Vidia Kusumah Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Safira Qisthina Ayuningtyas, Safira Qisthina Sahusilawane, Helena Afia Sandra, Aan Aryanti Sari Budi Moria Sembiring Sari Budi Moria Sembiring Sativa, Putriku Nadzira Satriani, Gloria I. Selly Ratna Sari Sholihin, Hidayatush Sri Nuryati Sri Sundari Sudarto Sudarto Syahril, Alfis Tia Oktaviani Upmal Deswira Vitas Atmadi Prakoso Wahyutomo Wahyutomo Wahyutomo, Wahyutomo Wibowo, Kesit Tisna Wicaksono, Aryo Wenang WIDANARNI WIDANARNI Wildan Nurussalam Wiyoto Wiyoto Yeni Elisdiana Yogi Himawan