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Thiamine supplement therapy improves ejection fraction value in stage ii heart failure patients Jikrona, Rafi; Suharjono, Suharjono; Ahmad, Abraham
Folia Medica Indonesiana Vol. 53, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

Thiamine, also called vitamin B1, is a water soluble vitamin that is involved in the formation of ATP in cells. The active metabolite of thiamine is a co-enzyme thiamine pyrophosphate (TPP) that plays an active role in carbohydrate metabolism and the formation of amino acid binding conjugates. Directly, thiamine may increase energy production in heart muscle cells through such mechanism, whereas in conditions of heart failure, a decrease in the contractility of heart muscle may be found. Therefore, thiamine supplementation is needed in stage II heart failure patients due to long-term use of furosemide that triggers the thiamine deficiency condition. Thiamine supplementation here is enabled to increase heart cells contractility which may ultimately increase the ejection fraction value of the heart muscle as a parameter of heart work efficiency measurement. The objective of this study was to evaluate the effect of thiamine administration as a therapeutic supplement in stage II of heart failure patients on the ejection fraction rate changes in pre and post thiamine supplement therapy for 28 days. The study was conducted using Cross Sectional Prospective Observational Analysis method between April and August 2016 in male patients with NYHA II heart failure who received furosemide therapy meeting the inclusion criteria at Heart Clinic Jemursari Islamic Hospital, Surabaya. Patients who fulfilled the inclusion and exclusion criteria in this study were 32 patients, 16 control patients and 16 treatment group patients between the ages of 35-75 years. During this study, after supplementation of thiamine 300 mg/day, 16 patients in the treatment group experienced an increase in ejection fraction rate (13.5%). The result of paired t test p=0.000 (p<α; α=0,05) showed significant difference between ejection fraction rate of pre and post thiamin supplementation. This study concluded that thiamine supplement therapy of 300 mg/day could increase the ejection fraction rate in patients of stage II heart failure.
Evaluation of Anti-inflammatory Activity of Mixture of Kencur Extract (Kaempferia galanga L.) with Glucosamine and Chondroitin In Vivo in Mice (Rattus Novergicus) Masyrobatin, Masyrobatin; Paramita, Angggun Putri; Sukardiman, Sukardiman; Suharjono, Suharjono
Jurnal Ilmiah Kesehatan (JIKA) Vol. 7 No. 2 (2025): Volume 7 Nomor 2 Agustus 2025
Publisher : Sarana Ilmu Indonesia (Salnesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36590/jika.v7i2.1027

Abstract

Glucosamine and chondroitin are commonly used in osteoarthritis therapy and are classified as SYSADOA. Both act as chondroprotective and anti-inflammatory agents, though their efficacy remains debated. Efforts have been made to enhance their anti-inflammatory effects by combining them with Kaempferia galanga L. (kencur) extract. This study aims to evaluate the anti-inflammatory effectiveness of a combination of kencur extract, glucosamine, and chondroitin in vivoin rats (Rattus norvegicus). The anti-inflammatory activity was tested on male white Wistar rats using the paw edema test. The increase in paw volume was measured and converted into the percentage of edema inhibition. Histopathological analysis to count neutrophil inflammatory cells was performed using Hematoxylin and Eosin (HE) staining. Data analysis was conducted on both the percentage of edema inhibition and the neutrophil cell counts. The results showed that the combination of glucosamine (45 mg/kg BW) and chondroitin (36 mg/kg BW) with kencur extract at doses of 600 mg, 900 mg, and 1200 mg/kg BW exhibited anti-inflammatory activity. The combination with 600 mg/kg BW of kencur extract showed a specific percentage of edema inhibition. The combination of glucosamine and chondroitin with 900 mg/kg BW of kencur extract showed the best anti-inflammatory activity, which was equivalent to 1200 mg/kg BW of kencur extract and superior to the effects of glucosamine and chondroitin alone.
Pengaruh Suhu & pH terhadap Laju Pertumbuhan Lima Isolat Bakteri Anggota Genus Pseudomonas yang Diisolasi dari Ekosistem Sungai Tercemar Deterjen di Sekitar Kampus Universitas Brawijaya Suriani, Sanita; Soemarno, Soemarno; Suharjono, Suharjono
Jurnal Pembangunan dan Alam Lestari Vol. 4 No. 1 (2013): Jurnal Pembangunan dan Alam Lestari
Publisher : Graduate School of Universitas Brawijaya

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Abstract

Tujuan dari penelitian ini adalah untuk mengetahui dampak temperature dan pH pada polulasi Pseudomonas pendegradasi limbah LAS. Hasil penelitian menunjukkan bahwa isolate bakteri P2.1 dapat tumbuh maksimal pada pH 8; isolate P3.2 dan C2.1 dapat tumbuh optimal pada pH 7, sedangkan isolate C2.2 dan C2.3 dapat tumbuh optimal pada pH 5, dan isolate P3.2 dapat tumbuh optimal pada suhu 40oC, isolate C2.1 C2.2 dan C.2.3 dapat tumbuh optimal pada temperature 30oC. Pada penelitian ini juga terdapat isolate yang tidak terpengaruh oleh perlakuan suhu. Hal ini dimungkinkan karena variasi suhu yang digunakan masih kisaran suhu optimum untuk pertumbuhan isolate, sedangkan isolate lain menunjukkan respon pertumbuhan akibat perlakuan suhu. Beberapa isolate menunjukkan pertumbuhan yang lambat, hal ini karena suhu yang digunakan untuk perlakuan dibawah suhu minimum atau diatas suhu maksimum untuk pertumbuhan bakteri. Kata kunci: Pseudomonas, LAS, temperatur
Use of Macrozoobenthos for Water Quality Monitoring in Ecotourism Area of Prafi River, Manokwari, West Papua Sinuraya, Sabarita; Arisoesilaningsih, Endang; Suharjono, Suharjono; Retnaningdyah, Catur
Journal of Indonesian Tourism and Development Studies Vol. 6 No. 2 (2018)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jitode.2018.006.02.05

Abstract

The aim of research is to monitor the water quality of some ecotourism sites along Prafi Rivers (Manokwari, West Papua), especially in Subsay, SP1 reservoir and SP3 reservoir using macro-zoobenthos biotic index, Pratiâ's index and some chemical-physical factors of water. Research was done from July to September 2016. Sampling was performed through Purposive Random Sampling. Macro-zoobenthos was collected from each sampling sites in three locations, (upstream, middle stream, downstream) using Surber net and hand net. Water quality was categorized based on Implicit Prati's Pollution index that is derived from DO, BOD5, pH, nitrate, orthophosphate and water temperature value. Macro-zoobenthos data was used to analyze the diversity index and six biotic indices. Result of the study showed that DO, BOD5, pH and nitrate value in all location meet water standard quality based on PP. no 82, 2001 class II (fisheries, recreation, animals husbandry and irrigation). The turbidity value in sites of upstream, middle stream and downstream Subsay and SP3 have meets standard water quality according to WHO for drinking water (<5 NTU). Based on the Prati's index, the water quality was ranging from excellent to acceptable categories with value 0.4-1.9. The taxa richness of macro-zoobenthos was 31 taxa. The shannon-wiener diversity index in all stations were more than 2, indicated that the water was not polluted. The water quality of all station showed very excellent up to moderates (based on FBI), very excellent up to very bad (HBI), and excellent up to less polluted (ASPT). Percentage of Ephemeroptera, Plecoptera, and Trichoptera (% EPT) in all station up to 50% (excellent), except in station SP3 upstream (40%). EPT richness value in all station excellent to moderate. It can be concluded that the water quality of Subsay in upstream, middle stream, downstream and downstream of SP3 was excellent.The middle stream of SP3 and middle stream of SPI has moderate quality, while the water quality in SP3 upstream, SPI upstream and SPI downstream was bad. Keyword: Ecotourism, water quality, Prafi River.
The Impact of Dissolved Nitrate and Phosphate on Maximum Growth Rate and Carrying Capacity of Oscillatoria in Intensive Shrimp (Litopenaeus vannamei) Farming Pond Situbondo, East Java, Indonesia Aliviyanti, Dian; Suharjono, Suharjono; Retnaningdyah, Catur
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1219.817 KB) | DOI: 10.21776/ub.jels.2016.007.01.11

Abstract

The aims of study are to analyze the effect of dissolved nitrate and phosphate content of the intensive shrimp farming pond Situbondo to maximum growth rate and carrying capacity of Oscillatoria population density in the laboratory. This is an experimental research method using completely randomized design with three replications. The treatment were variation of nitrate and phosphate concentration (N0; N6; N12; N24; N48; P0,2; P0,4; P0.8, P1.6  mg.L-1).  Experiment was done using a pure Oscillatoria culture in condition 25 watt lamp; 12 hours a day. The initial amount of Oscillatoria cells used for the treatment is 8 - 15 x 104 cell.mL-1. During the incubation process, chemical parameters were also observed including nitrate, phosphate, DO, and pH at the beginning of the incubation period. Oscillatoria cell was count every day until stationary phase for 30 days. Furthermore, a different test between treatments was conducted to determine levels of nitrate and phosphate in triggering the blooming of Oscillatoria using Oneway ANOVA analysis with SPSS Program. The results showed that the intensive shrimp pond waters of Situbondo already contain dissolved phosphate between 0.4 - 0.5 mg.L-1. Oscillatoria growth is strongly influenced by dissolved phosphate content in waters, phosphate levels of 0.2 - 0.4 or equivalent to the actual level of 0.6 - 0.7 mg.L-1 can cause the highest abundance of Oscillatoria that could endanger the ecosystem.  Keywords: dissolved phosphate, intensive shrimp farming, Nitrate, Oscillatoria.
Isolation and Identification of Antagonistic Bacterium against a Pathogens of Bacterial Tuber Rot of Amorphophallus muelleri Arfani, Nurfitri; Azrianingsih, Rodiyati; Suharjono, Suharjono
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1106.177 KB) | DOI: 10.21776/ub.jels.2018.008.03.06

Abstract

Rhizosphere bacteria have the ability to protect the host plants from the infection of pathogenic microorganisms. This study aimed to identify rhizosphere bacteria that were capable of inhibiting the growth of bacterial isolates that cause tuber rot of Amorphophallus muelleri. Rhizosphere bacteria were isolated using Nutrient Agar medium by pour plate method. Isolates were subjected to antagonistic assay against several bacterial isolates from the rotten tuber of A. muelleri using dual culture method. The potential isolate was identified based on 16S rDNA sequence. Isolate R7 showed the strongest inhibition to the growth of bacterial isolates from rotten tuber with an inhibition zone diameter of 19.66 mm. The 16S rDNA sequence of isolate R7 R7 was 99.7% similar to Delftia tsuruhatensis PCL1755. The isolate was potential to be developed as phytopathogen control agent.Keywords: Amorphophallus, antagonistic bacteria, rhizosphere bacteria, rotten tuber, 16S rDNA.
Evaluation of Proteolytic and Chitinolytic Activities of Indigenous Bacillus Species from Crab Shell Waste Dliyauddin, Moh; Ardyati, Tri; Suharjono, Suharjono
The Journal of Experimental Life Science Vol. 10 No. 1 (2020)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (768.558 KB) | DOI: 10.21776/ub.jels.2019.010.01.01

Abstract

The increase of crab meat export activities in Indonesia leads to the accumulation of crab shell waste in a massive amount that can naturally degrade and causing bad odor. Naturally, microorganisms will degrade this waste through fermentation and enzymatic reaction, including protease and chitinase due to its high content of chitin and protein. Bacillus is the most potential bacteria to degrade crab shell waste, and indigenous Bacillus from this waste can increase the degradation rates. The aims of this study were to evaluate the proteolytic and chitinolytic activities of indigenous Bacillus species from crab shell waste. Bacillus cereus BP14 and Bacillus licheniformis CK20 as the chitinolytic bacteria, and Bacillus subtilis AP9 and Bacillus licheniformis AP5 as the proteolytic bacteria were isolated from crab shell waste and identified based on the 16S rDNA sequences. The bacterial isolates were grown in skim milk broth for proteolytic characterization and colloidal chitin broth for chitinolytic characterization. The growth rates of each bacteria were determined through the growth curves. The enzymatic activities were determined based on the international standard for protease and chitinase enzyme activities together with growth curve sampling to determine the best incubation time for obtaining the highest enzymatic activities. From the shortest to the longest generation times of the Bacillus species obtained were B. subtilis AP9, B. licheniformis CK20, B. cereus BP14, and B. licheniformis AP5, respectively. The best incubation time for producing the highest enzymatic activity varied among species. However, the end of the logarithmic phase was similar. All of the Bacillus species obtained from this study exhibited chitinolytic and proteolytic activity. Therefore, it can be used as promising candidates of biodegradation agents inenhancing the degradation rates of crab shell wastes.
Plant Growth Promoting Endophytic Bacteria of Coffea canephora and Coffea arabica L. in UB Forest Pratiwi, Esti Rizkiana; Ardyati, Tri; Suharjono, Suharjono
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.07

Abstract

Plant Growth Promoting (PGP) Endophytic bacteria are used as an alternative biofertilizer to support soil health and plant productivity. This research aimed to isolate, analyze the potential, and identify the endophytic bacteria of Robusta and Arabica coffee plants as biofertilizer agents. Endophytic bacteria were isolated from the roots of coffee plants and tested for their potential to produce IAA, phosphate-solubilizing, and nitrogen fixation. Potential endophytic bacterial isolates were identified based on 16S rDNA sequence similarity. Total isolates from Robusta coffee consisting of ten IAA-producing bacteria, eight phosphate-solubilizing, and seven nitrogen fixation bacteria isolates. Total isolates from Arabica coffee roots were 12 isolates of IAA-producing bacteria, seven isolates of phosphate-solubilizing bacteria, and six isolates of nitrogen fixation bacteria. The highest potential of the isolate from Robusta roots was SS.E2 isolate to produce IAA 110.73 μg.mL-1; SS.P3 isolate to dissolve phosphate 4.42 μg.mL-1, and SS.N2 isolate to produce ammonium 3.15 μg.mL-1. The highest potential of the isolate from Arabica roots was SW.E9 isolate to produce IAA up to 257.16 μg.mL-1; SW.P5 isolate to dissolve phosphate up to 4.55 μg.mL-1; and SW.N6 isolate to produce ammonium up to 1.16 μg.mL-1. Isolates SS.E2, SW.E9, SS.P3, SW.P5, SS.N2, and SW.N6 were respectively identified as Bacillus cereus ATCC 14579, Bacillus cereus ATCC 14579, Rahnella aquatilis B35, Kluyvera intermedia TPY16, Rahnella aquatilis B35, and Pseudomonas tolaasii NCPPB 2192. Potential PGP isolates can be developed as biofertilizer agents for the coffee plant. Keywords: Coffee, Endophytic bacteria, IAA, Nitrogen, Phosphate
Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia Faizah, Mamluatul; Ardyati, Tri; Suharjono, Suharjono
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.09

Abstract

Palopo, South Sulawesi, is one of the traditional industrial centers of sago processing. The accumulation of sago pith waste around industrial sites can pollute the environment. Some microorganisms can degrade the cellulose in sago pith waste. This study was aimed to evaluate the indigenous cellulolytic bacteria from sago pith waste as a biodegradation agent. Bacteria were isolated from sago pith waste and grown on a 1% Carboxyl methyl cellulose (CMC) agar medium. The cellulolytic activity was analyzed semiquantitatively using 1% Congo red and quantitatively using the 3,5-Dinitrosalicylic Acid (DNS) method at pH variations of 4, 5, and 6. The potential isolate was identified based on 16S rDNA sequence similarity. This study obtained 21 bacterial isolates where six isolates were A1D, A1E, A1I, A1K, A2A, and B1A had the highest cellulolytic index at 0.82 - 1.13. Among those six isolates, the A1E isolate had the highest cellulolytic activity, 0.54 U.mL-1 at pH 6. The isolate A1E was identified as Burkholderia cepacia JCM 2799 with 99.73% similarity of 16S rDNA sequence. Keywords: Burkholderia cepacia, cellulolytic bacteria, cellulase enzyme, sago waste.
Analysis of Antibiotics on Patients Surgery, before and after Used Guidelines for Antibiotics (PPAB) Lisa Narulita; Riefkah Bilal; Mohammad Akram; Suharjono Suharjono
Journal of Pharmascience Vol. 7 No. 1 (2020): Jurnal Pharmascience
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v7i1.8074

Abstract

ABSTRAK Sejak    tahun    2011    World   Health    Organisasi   (WHO)   dan    Komite Pengedalian Resisten Antimikroba  (KPRA) sejak 2015, untuk  menetapkan kebijakan penggunaan antibiotik  dengan menyusun formularium dan  Pedoman Penggunaan Antibiotik (PPAB). Penggunaan antibiotik  berlebihan berkontribusi pada resistensi maka  penelitian ini bertujuan, menganalisis besarnya perbedaan penggunaan antibiotik  pada pasien rawat  inap bedah sebelum digunakan PPAB (Periode  Mei 2018)  dan  setelah  digunakan  PPAB (Periode  Mei 2019)  dengan ATC  / DDD (Defined   Daily  Dose).  Jenis  dan   rancangan  penelitian  adalah deskriptif cross-sectional dengan data retrospektif. Data  yang  diambil  meliputi peresepan antibiotik  di ruang  bedah. Data dianalisis dengan uji tanda (sign test) dan  uji wilcoxon  dengan hasil sign test yang  bertanda negatif sebanyak 9 dan bertanda positif sebanyak 2 dan  uji wilcoxon  menyatakan Sig.(2-tailed 0,062) (α=0,05) artinya   penggunaan  antibiotik   sebelum  dan setelah digunakan PPAB tidak berbeda atau  sama berdasarkan statistik. Tetapi dari  persentasi  penggunaan  antibiotik   terdapat 9  antibiotik   yang penggunaannya  menurun setelah digunakan PPAB  seperti sefoperason 50%, seftriaxon  52%,  sefuroxime  87%,  sepirom  90%,  siproflosasin  17%,  gentamisin 99%, levofloxasin 60%, metronidazol 79% dan  moxifloksasin 86%. Disimpulkan PPAB  perlu ditingkatkan dalam penggunaan antibiotik  di RSUD dr.H.Slamet Martodirdjo dan  penggunaa seftriakson  dapat meningkatkan  multi drug resistant. Dengan  demikian perlu  adanya evaluasi dan  monitoring dalam menerapkan PPAB. Kata kunci: ATC/DDD; Pasien Bedah; PPAB  ABSTRACT Since  2011  the  World Health  Organization (WHO) and  the  National Antimicrobial  Resistant Control  Committee (KPRA) in indonesia since 2015, to establish antibiotic use policies by developing formularies and  Antibiotic  Use Guidelines (PPAB). Excessive use of antibiotics contributing to resistance, this study aims, analyzing the  magnitude of  differences in antibiotic use patients surgery before PPAB (May 2018  period)  and after  PPAB (May 2019  period)  with ATC / DDD (Defined  Daily Dose). Type and  design  of the  study is  descriptive cross-sectional with retrospective. Data  taken  included prescribing antibiotics. Data  were  analyzed by sign test and  Wilcoxon test with 9 negative sign and  2 positive sign results and  Wilcoxon  test stated Sig. (2-tailed 0.062)>  (α = 0.05), meaning that  use of antibiotics before and  after  PPAB is not different or the same based on statistics. But from the percentage of antibiotic 9   decreased  after    PPAB,   such  as   cefoperazone  50%,   cefriaxone   52%, cefuroxime  87%,  cefpirome  90%,  ciprofloxacin  17%,  gentamicin  99%, levofloxacin 60%, metronidazole 79% and  moxifloxacin 86%.  Concluded that PPAB needs to be improved in the use of antibiotics in RSUD dr.H. Islamet Martodirdjo and the use of ceftriaxone can increase multi drug resistant. Thus the need for evaluation and monitoring in implementing PPAB. Keywords:  ATC/DDD; Surgical Patients; PPAB
Co-Authors A.A. Ketut Agung Cahyawan W Achmad Ridwan Achmad Ridwan, Achmad Aditya Maulana Perdana Putra Agriawan Sudirman Agriawan Sudirman Ahmad Nasir ahmad, abraham Aliviyanti, Dian Andri Geger Noviantoro Anita Purnamayanti Aprillia Wijaya, Stefani Arfani, Nurfitri Ari Wibowo Arief Bachtiar Arina Dery Puspitasari Assiddiqy, Miftah Farid Bagiyo, Harry Bagiyo, Harry Bagyo Yanuwiadi Bambang Eko Wahyono Bambang Sidharta Bambang Sudarmanto Budi Suprapti Budiatin, Aniek Setya Budiatin, Aniek Setya Catur Retnaningdyah Chairunnisa Chairunnisa Chris Alderman Chrismawan A, Chrismawan A Chrismawan Ardianto Dara Demi Sahayuna Dea Ayu Nabilah Debora Shinta Liana Dewi Wara Shinta Didik Hasmono Dinda Monika Nusantara Ratri Diniah, Melisa Nur Diyna Rusayliya Purwanto Djafar, Zaenab Dliyauddin, Moh Dwiyatna, Surya Ekanita Desiani Elvan Dwi Widyadi Emiliana Kasmudjiastuti Emy Sulistyo Astuti Endang Arisoesilaningsih Endang Retnowati Eni Susanti Faizah, Mamluatul Faizal Mustamin Fandyka Yufriza Ali Fasich Fasich Findari Megantari Fransiska Maria Christianty Gratia, Veronica Hari Prasetyo Hasria Alang Heru Purwanto Hubby, Hubby I Gede Edy Sagitha I Ketut Mandikin Ilham Rizqy Isnain Imam Faozi Imam Susilo Imamatus Shaleha Imron Solihin Indira D. Kharismawati Irfan Mustafa Irma Novrianti Irma Novrianti Irvina Harini Isnaeni Jaka Susila Jikrona, Rafi Joni Kusnadi Joni Wahyuhadi, Joni Jufri Ubrusun Junaidi Khotib Kenyo Alexandra Oktaviani Khaerani Khaerani Khusnul Fitri Hamidah Kusuma Arum Ningsih Kuwatno Kuwatno Lenny Octaviana Lestiono, . Lestiono, . Lewi Tigor Simorangkir Lisa Narulita Lisa Narulita Liziyyannida Liziyyannida M. Yusuf Assegaf Mahardian R, Mahardian R Mahardian Rahmadi Mahfudz Mahfudz Makitalentu, Feybe Marcha Debby Saraswati Mareta Rindang Andarsari Masyrobatin, Masyrobatin Medina, Farah Meita Rafika Fitriani Melasari, Wulan Panduwi Mohammad Akram Muhammad Aminuddin, Muhammad Muhammad Darwin Prenggono Muhammad Fathoni, Muhammad Mulja Hadi Santosa Narulita, Lisa Nasir, Ahmad Neldi, Vina Nia Kurniawan Nur Afni NUR CHAKIM Nur ‘Azah Nurmainah Nurmainah Nurmainah Nurmainah Nurrofik, Agus Paramita, Angggun Putri Paulus Sugianto Prasetyanti, Intan Kris Prastuti Asta Wulaningrum Pratita, Dian Galuh Pratiwi, Esti Rizkiana Primadi Avianto Purwanto, Diyna Rusayliya Putra Adi Purnama Rahman, Gilang Mauladi Rahmawati Raising Ria Rositasati Riefkah Bilal risha fillah fithria Risthanti, Reine Risa Rodiyati Azrianingsih Rosdiana, Eva Rudi Safarudin Rusdiana, Silvia Rusdiana, Silvia Safarudin, Rudi Sahayuna, Dara Demi Samirah Samirah, Samirah Samsulhadi, Wiwid Sanita Suriani Sari, Erni A Sari, Ratih Pratiwi Semedi S J Shafira Muti Ardiana Shahroni, Abdul Mutholib Shofia Ummu, Lathifa Simorangkir, Lewi Tigor Sindy, Sulih Probo Sinuraya, Sabarita SJ, Semedi Soemarno Soemarno Solihin, Imron Sukardiman Sulih Probo Sindy Sumarno . Sumarno Sumarno Suryadi Suryadi Susilo, Dwi Hari Sutrisnaningsih, Evy Sari Suwardi Suwardi Toetik Aryani Tri Ardyati Umami, Zahra Veronica Gratia Wanudya Atmajani WENNY PUTRI NILAMSARI, WENNY PUTRI Widhiati Widhiati Widyadi, Elvan Dwi Wiwid Samsulhadi Wulan Panduwi Melasari Yance Anas yance yance Yen Yen Ari Indrawijaya YOGA DWI JATMIKO Yogiarto Yogiarto Yulia Ayu Purnamasari Yulistiani Yulistiani Yulistiani Yulistiani, Yulistiani Yulistiani, . Yuniati T Yuniati T Yuniati T, Yuniati Yusfar, Yunarti Yustiana Yustiana, Yustiana Zamrotul Izzah Zulfiana, Risa