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Nt-probnp level changes after combination therapy with bisoprolol and ace-inhibitor in patient with heart failure Risthanti, Reine Risa; Aminuddin, Muhammad; Suharjono, Suharjono
Folia Medica Indonesiana Vol. 52, No. 4
Publisher : Folia Medica Indonesiana

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Abstract

Heart failure (HF) is the final common stage of many diseases of the heart. NT-proBNP levels are increased in HF and correlate well with ventricular wall stress and severity of HF. Combination therapy with Bisoprolol and ACE-inhibitor decreases NT-proBNP level in patient with HF. The use of Bisoprolol as a combination with ACE-inhibitor is still dominate in outpatient setting at Dr. Soetomo teaching hospital. The objective of this study is to analyze NT-proBNP level changes as an indicator in cardiac function after combination therapy with Bisoprolol and ACE-inhibitor in patient with HF.Methods: This study was prospective, observational and conducted in outpatient setting. Consecutive patients who meet the inclusion criteria of the study were included. Blood samples were taken at pre and 2 months post combination therapy with Bisoprolol and ACE-inhibitor, then NT-proBNP level was measured with IMMULITE®. There were 14 patients enrolled in this study (7 males, 7 females). The result showed that NT-proBNP 2 months post combination therapy with Bisoprolol and ACE-inhibitor is significantly decreased than baseline with mean baseline of NT-proBNP level is 4191.43 ± 4367.277 pg/ml to 2786.79 ± 2485.199 pg/ml (p=0.025). From a total 14 patients, 9 patients had NT-proBNP decreases >20% (20.1% – 56.4%) and 3 patients had NT-proBNP decreases <20% (1.8%, 6.6%, and 12.4%). There were 2 patients with NT-proBNP increases >40% (43.4% and 40.4%). In conclusion, there was a significant decreases in NT-proBNP level after 2 months combination therapy with Bisoprolol and ACE-inhibitor in patient with HF.
Effect of Atorvastatin in Lipid Profile Changes and Inflammation Marker TNF-alpha on Diabetes Patient with Dyslipidemia Melasari, Wulan Panduwi; Suharjono, Suharjono; Samsulhadi, Wiwid
Folia Medica Indonesiana Vol. 57, No. 1
Publisher : Folia Medica Indonesiana

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Abstract

Diabetes is one of the risk factors for cardiovascular disease (CVD). Diabetics patients have 2 to 4 times increased risk of cardiovascular disease compared with non-diabetics. TNF-alpha is a proinflammatory cytokine that can be used to determine the risk of atherosclerosis complications triggered by inflammation in diabetes. Statins are a class of HMG CoA reductase inhibitors that inhibit cholesterol biosynthesis and have pleioropic effects that inhibit the release of inflammatory cytokines like TNF-alpha and stabilize atherosclerotic plaques. This study aims to determine the effect of atorvastatin 20 mg/day for 30 days in reducing the lipid profile and TNF-alpha inflammatory markers in patients with diabetes dyslipidemia. Diabetes patient with dyslipidemia who included the inclusion criteria in this observational prospective cohorts studies treated with atorvastatin for 30 days (n = 19). The efficacy of statin therapy was measured by lipid profiles (LDL, TG, HDL, and total cholesterol) and TNF-alpha. The results of the study showed that atorvastatin decreased 40.55% of LDL levels, 15.34% of TG levels, and 30.70% oftotal cholesterol levels which statistically significant (P <0.05). As for HDL, there is an increase of 6.06% but statistically non-significant (P >0.05). TNF-alpha levels increased by 11.30% which statistically non-significant (P >0.05). The use of atorvastatin 20 mg for 30 days gave reduction in LDL, TG, and total cholesterol and increased in HDL. Atorvastatin does not have a reducing effect on TNF-alpha. There was no correlation between lipid profile changes with TNF-alpha changes.
Changes on Serum Troponin T Level before and after Taking Standard Therapy Medication in Heart Failure Patients Indrawijaya, Yen Yen Ari; Suharjono, Suharjono; Aminuddin, Muhammad; Retnowati, Endang; Rahman, Gilang Mauladi
Folia Medica Indonesiana Vol. 56, No. 1
Publisher : Folia Medica Indonesiana

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Abstract

Patients with advanced heart failure (NYHA FC III and IV heart failure) had positive cardiac troponin levels in previous cohort studies. In heart failure, cardiac troponin T (cTnT) is a biomarker that is sensitive to myocardial damage, especially myocardial necrosis. However, there is still little information regarding changes in cTnT levels during standard therapy. This prospective observational study is aimed at evaluating changes in cTnT levels before and after theadministration of standard therapy and evaluating symptom improvement before and after the administration of standard therapy in patients with severe heart failure. Measurement of cTnT levels and symptom improvement parameters before treatment was carried out on the first day of the inpatient and measurement after therapy was carried out on the last day of the inpatient. Sampling was done by consecutive sampling and found 30 patients in the inpatient room of the SMF Cardiovascular Disease, Dr. Soetomo Hospital, Surabaya during the months of May-July 2017. The results of the study obtained the average cTnT levels before therapy 33.48 + 31.88 pg/ml and the average cTnT levels after therapy 46.32 + 52.68 pg/ml. Based on the statistical difference test with the Wilcoxon sign-ranked test, there was no significant change in cTnT levels (p = 0.318). On the parameter of clinical symptom improvement, there was a significant decrease in pulse, respiratory rate, blood pressure, and mean arterial pressure before and after administration of therapy (p <0.05). There was no change in troponin T levels before and after the administration of therapy meant there was no worsening of myocardial necrosis.
Potassium monitoring in heart failure patients receiving combined therapy of captopril and furosemide Hubby, Hubby; Suharjono, Suharjono; Djafar, Zaenab
Folia Medica Indonesiana Vol. 52, No. 4
Publisher : Folia Medica Indonesiana

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In the treatment of heart failure is recommended the use of a combination of ACEI (captopril) with diuretics (furosemide), ACEI has the side effect of hyperkalemia 10-38% in hospitalized patients and 10% in outpatients, is increased in patients with renal failure and diabetes mellitus, the use diuretics occur hypokalemia 5-20% in outpatients. Hyperkalemia and hypokalemia both should be avoided in heart failure patients, because it can cause cardiac conduction disorders such as cardiac arrhythmias This study aims to monitor levels of potassium in the use of combination therapy with furosemide captopril in heart failure patients. The study was conducted using the method Prospective Observational Cross Sectional Analysis, conducted in January-May 2015 in heart failure patients male and female NYHA II-III receiving combination therapy with furosemide Captopril met the inclusion criteria were treated in the department CVCU Dr. Wahidin Sudirohusodo Makassar. Potassium levels were measured pre and post administration of the combination therapy. A total of 37 patients met the inclusion and exclusion criteria in this study, 31 male patients (83.8%) and 6 female patients (16.2%) aged between 35-80 years, during this study after administration of the combination therapy, 5 patients (13.5%) had hypokalemia (<3.5 mmol/L) and 2 patients (5.4%) experienced hyperkalemia (> 5 mmol/L) and 30 patients (81.1%) of normal potassium levels (3,5 to 5.0 mmol/L). The test results paired t test p = 0.432 (p> α;α = 0.05) showed no significant differences between potassium levels pre and post potassium levels. In conclusion, the use of a combination of captopril and furosemide therapy, the risk of hyperkalemia and hypokalemia not significant but need to be monitored levels of potassium and ECG patient.
Thiamine supplement therapy improves ejection fraction value in stage ii heart failure patients Jikrona, Rafi; Suharjono, Suharjono; Ahmad, Abraham
Folia Medica Indonesiana Vol. 53, No. 2
Publisher : Folia Medica Indonesiana

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Thiamine, also called vitamin B1, is a water soluble vitamin that is involved in the formation of ATP in cells. The active metabolite of thiamine is a co-enzyme thiamine pyrophosphate (TPP) that plays an active role in carbohydrate metabolism and the formation of amino acid binding conjugates. Directly, thiamine may increase energy production in heart muscle cells through such mechanism, whereas in conditions of heart failure, a decrease in the contractility of heart muscle may be found. Therefore, thiamine supplementation is needed in stage II heart failure patients due to long-term use of furosemide that triggers the thiamine deficiency condition. Thiamine supplementation here is enabled to increase heart cells contractility which may ultimately increase the ejection fraction value of the heart muscle as a parameter of heart work efficiency measurement. The objective of this study was to evaluate the effect of thiamine administration as a therapeutic supplement in stage II of heart failure patients on the ejection fraction rate changes in pre and post thiamine supplement therapy for 28 days. The study was conducted using Cross Sectional Prospective Observational Analysis method between April and August 2016 in male patients with NYHA II heart failure who received furosemide therapy meeting the inclusion criteria at Heart Clinic Jemursari Islamic Hospital, Surabaya. Patients who fulfilled the inclusion and exclusion criteria in this study were 32 patients, 16 control patients and 16 treatment group patients between the ages of 35-75 years. During this study, after supplementation of thiamine 300 mg/day, 16 patients in the treatment group experienced an increase in ejection fraction rate (13.5%). The result of paired t test p=0.000 (p<α; α=0,05) showed significant difference between ejection fraction rate of pre and post thiamin supplementation. This study concluded that thiamine supplement therapy of 300 mg/day could increase the ejection fraction rate in patients of stage II heart failure.
Isolation and Identification of Hydrocarbon-Degrading Bacteria that Tolerant to Saponin of Sapindus Rarak Plant Octaviany, Evi; Suharjono, Suharjono; Mustafa, Irfan
Jurnal Biodjati Vol 4 No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.4392

Abstract

A commercial saponin as biosurfactant can reduce the surface tension of water and increase of hydrocarbon degradation. However, this saponin can be toxic to some hydrocarbonoclastic bac-teria. This study aimed to obtain bacterial isolates that were tolerant and incapable to degrade saponin, and to identify them based on 16S rDNA sequence. Bacteria were isolated from petroleum contaminated soil in Wonocolo Village, Bojonegoro Regency, East Java, Indonesia. The soil samples were acclimated using Bushnell-Haas (BH) broth with 0.5% crude oil at room temperature for 3 weeks. The culture was spread onto BH agar incubated at 30°C for 7 days. The first screened, isolates were grown in nutrient broth with addition of sap-onin 0%, 8%, and 12% (v/v) then incubated at 30°C for three days. The bacterial cell density was measured using a spectrophotometer. Second screened, the isolates were grown on BH broth with addition of 0.5% saponin as a sole carbon source, and their cell densities were measured. The selected isolates were identified based on 16S rDNA sequences. Among 34 bacterial isolates, nine isolates were tol-erant to 12% saponin. Three bacterial isolates IHT1.3, IHT1.5, and IHT3.24 tolerant to high concentration of saponin and did not use this substance as growth nutrition. The IHT1.3, IHT1.5, and IHT3.24 isolates were identified as Ochrobactrum pseudogrignonense (99% similarity), Pseudomonas mendocina (99%), and Ochrobactrum pi-tuitosum; (97%), respectively. Those three selected isolates are good candidates as hydrocarbon-degrading bacteria to bioremediation of soil contaminated crude oil. However, the combined activity of bacteria and saponin to degrade hydrocarbon needs further study. 
Evaluation of Anti-inflammatory Activity of Mixture of Kencur Extract (Kaempferia galanga L.) with Glucosamine and Chondroitin In Vivo in Mice (Rattus Novergicus) Masyrobatin, Masyrobatin; Paramita, Angggun Putri; Sukardiman, Sukardiman; Suharjono, Suharjono
Jurnal Ilmiah Kesehatan (JIKA) Vol. 7 No. 2 (2025): Volume 7 Nomor 2 Agustus 2025
Publisher : Sarana Ilmu Indonesia (Salnesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36590/jika.v7i2.1027

Abstract

Glucosamine and chondroitin are commonly used in osteoarthritis therapy and are classified as SYSADOA. Both act as chondroprotective and anti-inflammatory agents, though their efficacy remains debated. Efforts have been made to enhance their anti-inflammatory effects by combining them with Kaempferia galanga L. (kencur) extract. This study aims to evaluate the anti-inflammatory effectiveness of a combination of kencur extract, glucosamine, and chondroitin in vivoin rats (Rattus norvegicus). The anti-inflammatory activity was tested on male white Wistar rats using the paw edema test. The increase in paw volume was measured and converted into the percentage of edema inhibition. Histopathological analysis to count neutrophil inflammatory cells was performed using Hematoxylin and Eosin (HE) staining. Data analysis was conducted on both the percentage of edema inhibition and the neutrophil cell counts. The results showed that the combination of glucosamine (45 mg/kg BW) and chondroitin (36 mg/kg BW) with kencur extract at doses of 600 mg, 900 mg, and 1200 mg/kg BW exhibited anti-inflammatory activity. The combination with 600 mg/kg BW of kencur extract showed a specific percentage of edema inhibition. The combination of glucosamine and chondroitin with 900 mg/kg BW of kencur extract showed the best anti-inflammatory activity, which was equivalent to 1200 mg/kg BW of kencur extract and superior to the effects of glucosamine and chondroitin alone.
Pengaruh Suhu & pH terhadap Laju Pertumbuhan Lima Isolat Bakteri Anggota Genus Pseudomonas yang Diisolasi dari Ekosistem Sungai Tercemar Deterjen di Sekitar Kampus Universitas Brawijaya Suriani, Sanita; Soemarno, Soemarno; Suharjono, Suharjono
Jurnal Pembangunan dan Alam Lestari Vol. 4 No. 1 (2013): Jurnal Pembangunan dan Alam Lestari
Publisher : Graduate School of Universitas Brawijaya

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Abstract

Tujuan dari penelitian ini adalah untuk mengetahui dampak temperature dan pH pada polulasi Pseudomonas pendegradasi limbah LAS. Hasil penelitian menunjukkan bahwa isolate bakteri P2.1 dapat tumbuh maksimal pada pH 8; isolate P3.2 dan C2.1 dapat tumbuh optimal pada pH 7, sedangkan isolate C2.2 dan C2.3 dapat tumbuh optimal pada pH 5, dan isolate P3.2 dapat tumbuh optimal pada suhu 40oC, isolate C2.1 C2.2 dan C.2.3 dapat tumbuh optimal pada temperature 30oC. Pada penelitian ini juga terdapat isolate yang tidak terpengaruh oleh perlakuan suhu. Hal ini dimungkinkan karena variasi suhu yang digunakan masih kisaran suhu optimum untuk pertumbuhan isolate, sedangkan isolate lain menunjukkan respon pertumbuhan akibat perlakuan suhu. Beberapa isolate menunjukkan pertumbuhan yang lambat, hal ini karena suhu yang digunakan untuk perlakuan dibawah suhu minimum atau diatas suhu maksimum untuk pertumbuhan bakteri. Kata kunci: Pseudomonas, LAS, temperatur
Use of Macrozoobenthos for Water Quality Monitoring in Ecotourism Area of Prafi River, Manokwari, West Papua Sinuraya, Sabarita; Arisoesilaningsih, Endang; Suharjono, Suharjono; Retnaningdyah, Catur
Journal of Indonesian Tourism and Development Studies Vol. 6 No. 2 (2018)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jitode.2018.006.02.05

Abstract

The aim of research is to monitor the water quality of some ecotourism sites along Prafi Rivers (Manokwari, West Papua), especially in Subsay, SP1 reservoir and SP3 reservoir using macro-zoobenthos biotic index, Pratiâ's index and some chemical-physical factors of water. Research was done from July to September 2016. Sampling was performed through Purposive Random Sampling. Macro-zoobenthos was collected from each sampling sites in three locations, (upstream, middle stream, downstream) using Surber net and hand net. Water quality was categorized based on Implicit Prati's Pollution index that is derived from DO, BOD5, pH, nitrate, orthophosphate and water temperature value. Macro-zoobenthos data was used to analyze the diversity index and six biotic indices. Result of the study showed that DO, BOD5, pH and nitrate value in all location meet water standard quality based on PP. no 82, 2001 class II (fisheries, recreation, animals husbandry and irrigation). The turbidity value in sites of upstream, middle stream and downstream Subsay and SP3 have meets standard water quality according to WHO for drinking water (<5 NTU). Based on the Prati's index, the water quality was ranging from excellent to acceptable categories with value 0.4-1.9. The taxa richness of macro-zoobenthos was 31 taxa. The shannon-wiener diversity index in all stations were more than 2, indicated that the water was not polluted. The water quality of all station showed very excellent up to moderates (based on FBI), very excellent up to very bad (HBI), and excellent up to less polluted (ASPT). Percentage of Ephemeroptera, Plecoptera, and Trichoptera (% EPT) in all station up to 50% (excellent), except in station SP3 upstream (40%). EPT richness value in all station excellent to moderate. It can be concluded that the water quality of Subsay in upstream, middle stream, downstream and downstream of SP3 was excellent.The middle stream of SP3 and middle stream of SPI has moderate quality, while the water quality in SP3 upstream, SPI upstream and SPI downstream was bad. Keyword: Ecotourism, water quality, Prafi River.
The Impact of Dissolved Nitrate and Phosphate on Maximum Growth Rate and Carrying Capacity of Oscillatoria in Intensive Shrimp (Litopenaeus vannamei) Farming Pond Situbondo, East Java, Indonesia Aliviyanti, Dian; Suharjono, Suharjono; Retnaningdyah, Catur
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1219.817 KB) | DOI: 10.21776/ub.jels.2016.007.01.11

Abstract

The aims of study are to analyze the effect of dissolved nitrate and phosphate content of the intensive shrimp farming pond Situbondo to maximum growth rate and carrying capacity of Oscillatoria population density in the laboratory. This is an experimental research method using completely randomized design with three replications. The treatment were variation of nitrate and phosphate concentration (N0; N6; N12; N24; N48; P0,2; P0,4; P0.8, P1.6  mg.L-1).  Experiment was done using a pure Oscillatoria culture in condition 25 watt lamp; 12 hours a day. The initial amount of Oscillatoria cells used for the treatment is 8 - 15 x 104 cell.mL-1. During the incubation process, chemical parameters were also observed including nitrate, phosphate, DO, and pH at the beginning of the incubation period. Oscillatoria cell was count every day until stationary phase for 30 days. Furthermore, a different test between treatments was conducted to determine levels of nitrate and phosphate in triggering the blooming of Oscillatoria using Oneway ANOVA analysis with SPSS Program. The results showed that the intensive shrimp pond waters of Situbondo already contain dissolved phosphate between 0.4 - 0.5 mg.L-1. Oscillatoria growth is strongly influenced by dissolved phosphate content in waters, phosphate levels of 0.2 - 0.4 or equivalent to the actual level of 0.6 - 0.7 mg.L-1 can cause the highest abundance of Oscillatoria that could endanger the ecosystem.  Keywords: dissolved phosphate, intensive shrimp farming, Nitrate, Oscillatoria.
Co-Authors A.A. Ketut Agung Cahyawan W Achmad Ridwan Achmad Ridwan, Achmad Agriawan Sudirman Ahmad Nasir ahmad, abraham Aliviyanti, Dian Andri Geger Noviantoro Anita Purnamayanti Aprillia Wijaya, Stefani Arfani, Nurfitri Ari Wibowo Arief Bachtiar Arina Dery Puspitasari Assiddiqy, Miftah Farid Bagiyo, Harry Bagiyo, Harry Bagyo Yanuwiadi Bambang Eko Wahyono Bambang Sidharta Bambang Sudarmanto Budi Suprapti Budiatin, Aniek Setya Budiatin, Aniek Setya Catur Retnaningdyah Chairunnisa Chairunnisa Chris Alderman Chrismawan A, Chrismawan A Chrismawan Ardianto Dea Ayu Nabilah Debora Shinta Liana Dewi Wara Shinta Didik Hasmono Dinda Monika Nusantara Ratri Diniah, Melisa Nur Djafar, Zaenab Dliyauddin, Moh Dwiyatna, Surya Ekanita Desiani Elvan Dwi Widyadi Emiliana Kasmudjiastuti Emy Sulistyo Astuti Endang Arisoesilaningsih Endang Retnowati Eni Susanti Faizah, Mamluatul Faizal Mustamin Fandyka Yufriza Ali Fasich Fasich Fransiska Maria Christianty Gratia, Veronica Hari Prasetyo Hasria Alang Heru Purwanto Hubby, Hubby I Gede Edy Sagitha I Ketut Mandikin Ilham Rizqy Isnain Imam Faozi Imam Susilo Indira D. Kharismawati Irfan Mustafa Irma Novrianti Irma Novrianti Irvina Harini Isnaeni Jaka Susila Jikrona, Rafi Joni Kusnadi Joni Wahyuhadi, Joni Jufri Ubrusun Junaidi Khotib Kenyo Alexandra Oktaviani Khaerani Khaerani Khusnul Fitri Hamidah Kusuma Arum Ningsih Kuwatno Kuwatno Lestiono, . Lestiono, . Lewi Tigor Simorangkir Lisa Narulita Lisa Narulita Liziyyannida Liziyyannida M. Yusuf Assegaf Mahardian R, Mahardian R Mahardian Rahmadi Mahfudz Mahfudz Makitalentu, Feybe Marcha Debby Saraswati Mareta Rindang Andarsari Masyrobatin, Masyrobatin Medina, Farah Meita Rafika Fitriani Melasari, Wulan Panduwi Mohammad Akram Muhammad Aminuddin, Muhammad Muhammad Fathoni, Muhammad Mulja Hadi Santosa Narulita, Lisa Nasir, Ahmad Neldi, Vina Nia Kurniawan Nur Afni NUR CHAKIM Nur ‘Azah Nurmainah Nurmainah Nurrofik, Agus Octaviany, Evi Paramita, Angggun Putri Paulus Sugianto Prasetyanti, Intan Kris Prastuti Asta Wulaningrum Pratita, Dian Galuh Pratiwi, Esti Rizkiana Primadi Avianto Purwanto, Diyna Rusayliya Rahman, Gilang Mauladi Rahmawati Raising Ria Rositasati Riefkah Bilal risha fillah fithria Risthanti, Reine Risa Rodiyati Azrianingsih Rosdiana, Eva Rudi Safarudin Rudi Wardana Rusdiana, Silvia Rusdiana, Silvia Safarudin, Rudi Sahayuna, Dara Demi Samirah Samirah Samirah Samirah, Samirah Samsulhadi, Wiwid Sanita Suriani Sari, Erni A Semedi S J Shafira Muti Ardiana Shahroni, Abdul Mutholib Shofia Ummu, Lathifa Simorangkir, Lewi Tigor Sindy, Sulih Probo Sinuraya, Sabarita SJ, Semedi Soemarno Soemarno Solihin, Imron Sudirman, Agriawan Sukardiman Sumarno . Sumarno Sumarno Suryadi Suryadi Susilo, Dwi Hari Sutrisnaningsih, Evy Sari Suwardi Suwardi Toetik Aryani Tri Ardyati Umami, Zahra Veronica Gratia Wanudya Atmajani WENNY PUTRI NILAMSARI Widhiati Widhiati Widyadi, Elvan Dwi Wiwid Samsulhadi Wulan Panduwi Melasari Yance Anas yance yance Yen Yen Ari Indrawijaya YOGA DWI JATMIKO Yogiarto Yogiarto Yulia Ayu Purnamasari Yulistiani Yulistiani Yulistiani Yulistiani, Yulistiani Yulistiani, . Yuniati T Yuniati T Yuniati T, Yuniati Yusfar, Yunarti Yustiana Yustiana, Yustiana Zamrotul Izzah Zulfiana, Risa