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The presence of disease in growing spiny lobster Panulirus spp. in floating net cages at Tanjung Putus Island, Lampung Sukenda; Lastriliah, Mira; Widanarni, Widanarni; Effendi, Irzal; Hidayatullah, Dendi
Jurnal Akuakultur Indonesia Vol. 23 No. 1 (2024): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.23.1.24-35

Abstract

Spiny lobster is a member of crustaceans that has high economic value. The demand for export markets in Asian, European and American countries as well as locally, is quite high with an expensive selling price. However, there are obstacles that often occur in spiny lobster rearing cultivation, namely infectious diseases that will cause high mortality. The purpose of this study was to investigate infectious diseases in rearing spiny lobster Panulirus spp. that were kept in floating net cages at Tanjung Putus, Lampung. The research method involved sampling five spiny lobsters in floating net cages, observation of clinical symptoms, bacterial isolation, identification of bacteria, detection of Milky Hemolymph Disease of Spiny Lobster (MHD-SL) through polymerase chain reaction (PCR) analysis and histopathology. The sampling location was at PT. Saibatin Perikanan Indonesia. The bacteria Vibrio parahaemolyticus and Vibrio alginolyticus were detected. Five lobsters were infected by MHD-SL, characterized by milky white hemolymph. All five lobster samples were positively infected with Milky Hemolymph Disease of Spiny Lobster (MHD-SL) through PCR analysis. Histopathological observations showed pathological microanatomical changes in the lobster hepatopancreas tissue, indicating that the tissue changes, in the form of encapsulation and infiltration were due to Rickettsia-like bacteria (RLB) infection. The management of spiny lobster stocking density, maintenance biosecurity, and increased lobster immunity must be implemented in order to prevent MHD-SL disease in floating net cages. Keywords: bacteria, histopathology, MHD-SL, Panulirus spp. ABSTRAK Lobster merupakan anggota crustacea yang memiliki nilai ekonomis yang tinggi. Permintaan pasar ekspor pada negara Asia, Eropa, dan Amerika maupun lokal cukup tinggi dengan nilai jual yang mahal. Akan tetapi, terdapat kendala yang sering terjadi pada budidaya pembesaran lobster yaitu infeksi penyakit yang akan menyebabkan tingginya mortalitas Tujuan penelitian ini adalah untuk menginvestigasi penyakit infeksi pada pembesaran lobster Panulirus spp. yang dipelihara di keramba jaring apung Tanjung Putus, Lampung. Metode penelitian ini meliputi pengambilan sampel lobster sebanyak lima ekor dalam karamba jarring apung (KJA), pengamatan gejala klinis, isolasi bakteri, identifikasi bakteri, deteksi Milky Hemolymph Disease of Spiny Lobster (MHD-SL) melalui analisis Polymerase Chain Reaction (PCR), dan histopatologi. Lokasi pengambilan sampel di PT. Saibatin Perikanan Indonesia, Lampung. Terdapat bakteri Vibrio parahaemolyticus dan V. alginolyticus. Lima ekor lobster terinfeksi oleh MHD-SL yang ditandai dengan hemolim berwarna putih susu. Lima sampel lobster positif terinfeksi penyakit Milky Hemolymph Disease of Spiny Lobster (MHD-SL) melalui analisis PCR. Hasil pengamatan histopatologi terdapat perubahan mikroanatomi patologi pada jaringan hepatopankreas lobster menunjukkan bahwa adanya perubahan jaringan berupa enkapsulasi dan infiltrasi karena adanya infeksi Rickettsia-like bacteria (RLB). Pengelolaan kepadatan penebaran lobster, biosekuritas pemeliharaan, dan peningkatan imunitas lobster harus ditingkatkan untuk mencegah penyakit MHD-SL di KJA. Kata kunci: bakteri, histopatologi, MHD-SL, Panulirus spp.
Distribution of Vibrio parahaemolyticus in pacific white shrimp Litopenaeus vannamei through immersion as a natural infection model Sari Anggraeni, Sukma; Sukenda; Nuryati, Sri; Wahjuningrum, Dinamella
Jurnal Akuakultur Indonesia Vol. 23 No. 2 (2024): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.23.2.176-188

Abstract

Vibrio parahaemolyticus is a pathogenic bacterium that indusces mortality is pacific white shrimp. This study aimed to analyze the distribution of V. parahaeomyticus in shrimp through immersion and evaluate, polymerase chain reaction, histopathology, and immune response. The study employed two treatments with four replicates each. Shrimp were infected through immersion with V. parahaemolyticus at a concentration of 107 CFU/mL for 30 minutes, followed by transferring the shrimp to a rearing container. Gill, hepatopancreas, gut, and hemolymph samples were collected at 6, 12, and 24 hours post-infection. Infected shrimp exhibited clinical symptoms such as a pale body and hepatopancreas, along with empty gut. Shrimp mortality reached 97.08%, while no mortality occurred in the control grouph. The abundance of V. parahaemolyticus in organs exceeded 105 CFU/mL. Histopathological examination revealed mild, moderate, and severe damage. PCR confirmation tests at 12 and 24 hours yielded positive results, with a DNA band at 230 bp. Immune responses, including total hemocytes, phagocytic activity, phenoloxidase, and respiratory burst, demonstrated significantly different results (P<0.05). It was concluded that the distribution of V. parahaemolyticus in the shrimp body, specifically in the gills, hepatopancreas, and gut, exceed 105 CFU/mL, as confirmed by a 230 bp DNA band in the PCR test. Tissue damage, manifested as lesions and necrosis, occurred in the gills, hepatopancreas, and gut. Additionally, the imun response of Vibrio-infected shrimp was found to be lower than that of uninfected shrimp (negative control). Keywords: distribution, immersion, Litopenaeus vannamei, Vibrio parahaemolyticus ABSTRAK Vibrio parahaemolyticus adalah bakteri patogen yang menyebabkan kematian pada udang vaname. Penelitian ini bertujuan menganalisis distribusi V. parahaemolyticus pada udang melalui perendaman serta kerusakan yang ditimbulkan melalui pengamatan gejala klinis, angka lempeng total bakteri, polymerase chain reaction, histopatologi dan respons imun. Penelitian menggunakan dua perlakuan dan empat ulangan. Udang diinfeksi melalui perendaman menggunakan V. parahaemolyticus 107 CFU/mL selama 30 menit, kemudian udang dipindah pada wadah pemeliharaan. Pengambilan organ insang, hepatopankreas, usus dan hemolim dilakukan pada jam ke-6, 12, dan 24 pascainfeksi. Udang terinfeksi memiliki gejala klinis seperti tubuh dan hepatopankreas pucat, usus kosong. Mortalitas udang mencapai 97.08% dan tidak ada kematian pada udang kontrol. Kelimpahan V. parahaemolyticus pada organ lebih dari 105 CFU/mL. Pengamatan histopatologi menunjukkan kerusakan ringan, sedang, hingga parah. Uji konfirmasi PCR jam ke-12 dan ke-24 menunjukkan hasil positif pada 230 bp. Respons imun seperti total hemosit, aktivitas fagositik, phenoloxidase, respiratory burst menujukkan hasil yang berbeda nyata (P<0.05). Disimpulkan bahwa distribusi V. parahaemolyticus di dalam tubuh udang pada terdapat pada insang, hepatopankreas dan usus lebih dari 105 CFU/mL dan terkonfirmasi uji PCR pada pita DNA 230 bp. Kerusakan jaringan seperti lesi dan nekrosis terjadi pada insang, hepatopankreas, dan usus. Respons imun udang yang terinfeksi Vibrio lebih rendah dibandingkan udang yang tidak terinfeksi (kontrol negatif). Kata kunci: distribusi, Litopenaeus vannamei, perendaman, Vibrio parahaemolyticus
Potential of Eurycoma longifolia, Curcuma zedoaria, and Allium sativum extracts as phytobiotics for shrimp health Iqbal Kurniawinata, Mohamad; Sukenda, Sukenda; Wahjuningrum, Dinamella; Widanarni, Widanarni; Ekasari, Julie
Jurnal Akuakultur Indonesia Vol. 24 No. 1 (2025): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.24.1.71-81

Abstract

This study evaluated the efficacy of Eurycoma longifolia, Curcuma zedoaria, and Allium sativum in improving the immune response and resistance of whiteleg shrimp to prevent Vibrio parahaemolyticus infection. The study consisted of two phases, an in vitro phase to determine the compounds contained in three medicinal plants as antibacterials, followed by an in vivo phase to evaluate the effect of the medical plant extract on immune response and robustness against V. parahaemolyticus. The results from the first phase revealed that bioactive compounds present in E. longifolia were more varied and had higher concentrations with a lower bactericidal value when compared to those found in C. zedoaria or A. sativum. In the second phase of the experiment, the medicinal plant extract was added to the feed with a dose that was determined according to the first phase results. The treatments tested in the second phase were 1.6% E. longifolia extract dietary addition (EL16), 6.4% C. zedoaria extract dietary addition (CZ64), 6.4% A. sativum extract dietary addition (AS64) and phytobiotics mixture of 1:1:1 (C1) dietary addition, as well as no phytobiotic for negative control treatment and positive control. The results from the second stage demonstrated that dietary phytobiotic extract addition enhances the immunological responses and improves the shrimp survival against V. parahaemolyticus challenge compared to the control group. In conclusion, E. longifolia, C. zedoaria, and A. sativum showed different bioactive compound profiles, which affect their efficacy against V. parahaemolyticus, with EL16 showing higher efficacy. Keywords: A. sativum, C. zedoaria, E. longifolia, Penaeus vannamei, Phytobiotic ABSTRAK Penelitian ini mengevaluasi efikasi Eurycoma longifolia, Curcuma zedoaria, dan Allium sativum dalam meningkatkan respon imun dan resistensi udang vaname untuk mencegah infeksi Vibrio parahaemolyticus. Penelitian ini terdiri dari dua tahap: tahap in vitro untuk menentukan senyawa yang terkandung dalam tiga tanaman obat sebagai antibakteri, diikuti oleh tahap in vivo untuk mengevaluasi efek ekstrak tanaman obat terhadap respon imun dan ketahanan terhadap V. parahaemolyticus. Hasil dari tahap pertama mengungkapkan bahwa senyawa bioaktif yang ada dalam E. longifolia lebih bervariasi dan memiliki konsentrasi yang lebih tinggi dengan nilai bakterisida yang lebih rendah jika dibandingkan dengan yang ditemukan di C. zedoaria atau A. sativum. Pada percobaan tahap kedua, ekstrak tanaman obat ditambahkan ke pakan dengan dosis yang ditentukan sesuai dengan hasil tahap pertama. Perlakuan yang diuji pada tahap kedua adalah penambahan 1,6% ekstrak E. longifolia dalam pakan (EL16), penambahan 6,4% ekstrak C. zedoaria dalam pakan (CZ64), penambahan 6,4% ekstrak A. sativum dalam pakan (AS64) dan campuran fitobiotik 1:1:1 (C1) dalam pakan, serta tanpa fitobiotik untuk perlakuan kontrol negatif dan kontrol positif. Hasil dari tahap kedua menunjukkan bahwa penambahan ekstrak fitobiotik dalam pakan meningkatkan respons imunologi dan memperbaiki kelangsungan hidup udang terhadap tantangan V. parahaemolyticus dibandingkan dengan kelompok kontrol. Sebagai kesimpulan, E. longifolia, C. zedoaria, dan A. sativum menunjukkan profil senyawa bioaktif yang berbeda, yang mempengaruhi efikasinya terhadap V. parahaemolyticus, dengan EL16 menunjukkan efikasi yang lebih tinggi. Kata kunci: Allium sativum, Curcuma zedoaria, Eurycoma. longifolia, fitobiotik, Penaeus vannamei
Meningkatkan kemampuan pemahaman siswa dengan menggunakan metoda penemuan terbimbing pada pembelajaran geometri kelas 8 Sukenda Sukenda; Uba Umbara; Evan Farhan Wahyu Puadi
JUMLAHKU: Jurnal Matematika Ilmiah STKIP Muhammadiyah Kuningan Vol 4 No 1 (2018): Edisi Vol. 4 No. 1 Mei
Publisher : Program Studi Pendidikan Matematika STKIP Muhammadiyah Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The purpose of the study was to investigate whether Guided Finding Methods could improve students' understanding of geometry in class 8. The method used is Classroom Action Research. The research was done at SMP Negeri 2 Ciwaru located at Jl. Raya Sumberjaya, Desa Citikur, Kecamatan Ciwaru, Kabupaten Kuningan. The subjects of the researchs were the students of SMP Negeri 2 Ciwaru class 8 academic year 2016/2017. They were chosen because after consulting with mathematics teachers that this had major problems regarding the comprehension ability of mathematical concepts. Data collection techniques: observation, interview and questionnaire. The research was done in 2 cycles, each cycle was done in 3 sessions. Each session includes 4 stages: planning, action, observation and reflection. The results of the research as follows: Learning with guided discovery method can improve students' understanding 73.82%. With the following details on the first cycle students who reached the KKM 17 students (62.54%) with an average score of 65.74. In cycle II the number of KKM 23 students (85.19%) the average score in cycle II was 79.26. Based on the results of the research it is advisable to use guided discovery methods in improving students' comprehension skills.
Making Learning Module Using Google Spaces for Kindergarten Teachers in Pangandaran Based on LMS (Learning Management System) Wahyu Wibowo, Ari Purno; Laksana, Eka Angga; Sukenda, Sukenda; Yustim, Benny
Brilliance: Research of Artificial Intelligence Vol. 5 No. 1 (2025): Brilliance: Research of Artificial Intelligence, Article Research May 2025
Publisher : Yayasan Cita Cendekiawan Al Khwarizmi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47709/brilliance.v5i1.5770

Abstract

Learning process Children of kindergarten age tend to get bored easily and must use a different approach, because at an early age children will get tired easily if taught something tire Computers with attractive visual displays, animations, and sounds can make the learning process feel like playing, so that they are more enthusiastic and motivated. to overcome this, it is necessary to create a more innovative multimedia-based teaching system on the material taught must have parameters that are visual, audio and interactive material, so that the material delivered can be easily measured according to learning outcomes, in this study the author tried to use the help of open source applications based on Google Spacess and LMS (Learning Management System) which can be used by kindergarten teachers in the Pangandaran area, by using this Google Spaces assistance, it turns out that teachers can create the same and more interactive teaching material, in the end with the help of digital media, the material can be adjusted to the individual abilities of each child's speed and can be evaluated directly because for example children who have mastered the material can proceed to the next stage, while on the contrary students who are left behind in learning can repeat more relaxedly repeating the learning, then with the help of teachers and the student learning process can be done independently and not limited by time, because the LMS module can be accessed at any time by students. From the results of the training held for 65 kindergarten teachers, 85% agreed that this LMS application was very helpful in teaching and easy to configure and re-develop.
Kebijakan Inovatif untuk Akuakultur Berkelanjutan: Optimalisasi Peran Bivalvia dalam Pengolahan Limbah Pras, Eva Prasetiyono; Nirmala, Kukuh; Supriyono, Eddy; Sukenda; Hastuti, Yuni Puji
Policy Brief Pertanian, Kelautan, dan Biosains Tropika Vol. 7 No. 2 (2025): Policy Brief Pertanian, Kelautan, dan Biosains Tropika
Publisher : Direktorat Kajian Strategis dan Reputasi Akademik IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agro-maritim.0702.1272-1276

Abstract

Pertumbuhan industri tambak udang intensif di Indonesia telah memberikan kontribusi ekonomi yang signifikan. Namun tambak udang juga menimbulkan permasalahan lingkungan akibat buangan kaya nutrien seperti amonium, nitrat, ortofosfat, partikel organik dan tersuspensi. Kandungan nutrien yang tinggi dalam buangan tambak dapat memicu ledakan populasi mikroalga, menurunkan kualitas lingkungan dan terganggunya keseimbangan ekosistem perairan. Pemerintah telah mengeluarkan kebijakan yang mewajibkan setiap tambak udang memiliki unit pengolahan limbah sebagai bentuk pengendalian dampak lingkungan. Namun, implementasi kebijakan ini menghadapi tantangan besar terkait dengan besarnya biaya investasi dan operasional instalasi pengolahan limbah tersebut. Upaya untuk memberikan solusi alternatif yang lebih efisien dan ramah lingkungan harus dilakukan, diantaranya dengan menggunakan bivalvia. Pemanfaatan bivalvia— seperti kerang darah—dapat menjadi pendekatan biotreatment yang efektif. Bivalvia berperan sebagai filter feeder yang mampu mengasimilasi mikroalga dan partikel nutrien di dalam air. Filtrasi menggunakan kerang darah terbukti mampu menurunkan konsentrasi mikroalga dan nutrien, masing-masing sebesar 72,80% untuk mikroalga, 36,78% untuk amonia, 50,00% untuk nitrit, 78,77% untuk nitrat, dan 89,13% untuk ortofosfat.. Rekomendasi alternatif kebijakan diarahkan pada integrasi penggunaan bivalvia dalam sistem pengolahan limbah tambak, dan penyusunan pedoman teknis biotreatment yang lebih efisien sebagai bagian dari strategi pengelolaan limbah berkelanjutan. Strategi ini tidak hanya memperkuat upaya perlindungan lingkungan, tetapi juga membuka peluang ekonomi tambahan melalui produksi bivalvia sebagai komoditas konsumsi.
Cyber Pandemic – The New Cybersecurity Risks Sukenda, Sukenda; Zulpratita, Ulil Surtia; Muttaqin, Helmy Faisal; Wahyu, Ari Purno; Yustim, Benny
Brilliance: Research of Artificial Intelligence Vol. 4 No. 2 (2024): Brilliance: Research of Artificial Intelligence, Article Research November 2024
Publisher : Yayasan Cita Cendekiawan Al Khwarizmi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47709/brilliance.v4i2.4759

Abstract

We are amidst a digital pandemic. In 2020, COVID-19 sped up a change towards remote working and the product being utilized for these assaults has become more straightforward to execute, ransom ware assaults have risen quickly and keep on speeding up in 2021. The COVID-19 pandemic has changed both the actual world and the computerized space, where organizations and associations are being gone up against with overwhelming online protection challenges for which not many were prepared or prepared to confront. Attributable to the extreme change in working conditions, cyber attacks and information extortion presently rank third among the best worries of business pioneers, as detailed in the World Economic Forum's COVID-19 Risks Outlook. The likelihood of malignant digital movement is considerably more upsetting thinking about that 53% of organizations have never pressure tried their systems. The key focus point from these and a large group of other alarming probabilities is that readiness for any kind of digital emergency whatsoever levels of an association is critical. Top administration, network protection trained professionals and each representative should know how to treat an emergency hit. Cyber security centers around securing information, yet it is presently not adequate; organizations need cyber resilience.
Isolation, Characterization, and In Vitro Evaluation of Bacteriophages for Controlling the Fish Pathogen Aeromonas hydrophila Nasri Julaini; Wahjuningrum, Dinamella; Widanarni; Sukenda
Jurnal Ilmiah Perikanan dan Kelautan Vol. 18 No. 1 (2026): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v18i1.79707

Abstract

Graphical Abstract   Highlight Research 1. A lytic bacteriophage was successfully isolated from catfish pond water in Dramaga, Bogor, with a high density of 5.68 × 10⁹ PFU/mL. 2. Characterization revealed round to irregular plaque morphology with high clarity and diameters reaching 0.35 cm. 3. The bacteriophage exhibited high host specificity, being effective only against the A. hydrophila AH03 isolate from the AAHL collection. 4. Treatment at MOI 10 resulted in more effective inhibition of A. hydrophila growth compared to other treatments.   Abstract Aquaculture plays a vital role in global protein supply, yet its increasing production faces disease-related challenges, particularly A. hydrophila infections. This pathogen causes Motile Aeromonas Septicemia (MAS), leading to mass mortality in catfish and significant economic losses. While antibiotics have been the primary solution, their use is now restricted due to antimicrobial resistance, necessitating safe and sustainable alternatives. Phage have emerged as environmentally friendly, specific biocontrol agents to combat such infections. This study aimed to evaluate the effectiveness of lytic phage in controlling the fish pathogen A. hydrophila through an in vitro approach. The research stages included phage isolation (density assay), characterization (plaque morphology and host range testing), and evaluation of phage against A. hydrophila (bacteriolytic efficacy assay). A completely randomized design was employed, with six treatments and three replicates, media control (K-), A. hydrophila control (K+), antibiotic control (enrofloxacin 0.1 mg/mL, KA), and phage treatments at multiplicities of infection (MOI) of 0.1, 1, and 10 (P0.1, P1, P10) for the bacteriolytic efficacy assay. Phage isolated from catfish ponds in Dramaga, Bogor, exhibited a high titer (5.68 × 10⁹ PFU/mL) and formed clear, round plaques (0.17-0.35 cm diameter). They demonstrated a narrow host range, lysing only 1 of 10 A. hydrophila isolates. In efficacy assays, phage treatment at an MOI of 10 significantly reduced bacterial density (p < 0.05) at 24 hours post infection, decreasing the OD 600 nm by 38.7% compared to the positive control. These results indicate the potential of these phage as an antibiotic alternative for controlling A. hydrophila in aquaculture.    
Isolation of Lytic Bacteriophages infected Indonesian-strain Vibrio parahaemolyticus and its Protective Effects on Brine Shrimp (Artemia sp.) Wahjuningrum, Dinamella; Ramhirez, Putri Shandra; Nuzullia, Laely; Yuhana, Munti; Sukenda, Sukenda; Nasrullah, Hasan
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 1 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.vi.67419

Abstract

Graphical Abstract Highlight Research vB_Vp_TSK01 and vB_Vp_JKT02 a specific parahaemolyticus bacteriophage were isolated from the shrimp aquaculture water. Compared to control vB_Vp_TSK01 reduced 11.46±1.35% of bacterial growth, and by 9.86±5.92% for vB_Vp_JKT02 treatment. Their cocktail had the highest parahemolyticus growth inhibition by 30.92±3.89%. The phage treatment increased the survival of the Artemia by 28.57% compared to infection control.     Abstract Acute Hepatopancreatic Necrosis Disease (AHPND) caused by V. parahaemolyticus infection was one of the major diseases in shrimp culture in recent years. The Vibrio could also affect the survival of Artemia as the shrimp’s main live feed in the hatchery and they become the possible carrier for the AHPND. Phage therapy in shrimp aquaculture could reduce the application of the antibiotic as an antibacterial agent for the AHPND. The present study aimed to isolate the specific lytic phage for the Indonesian strain of V. parahaemolyticus (Vp) and evaluate the phage therapy for the brine shrimp Artemia infected with the Vp. The Vp-specific phage was isolated from the shrimp farm’s water at Tasikmalaya, and North Jakarta City, Indonesia. After isolation and plaque assay, brine shrimp were used as a model to evaluate the phages’ anti-Vibrio activity The Vp-lytic phage was successfully isolated from shrimp culture water at North Jakarta and Tasikmalaya (Vb_Vp_TSK01 and Vb_Vp_JKT01, respectively) and the results showed that both isolated phages and their cocktails were capable to inhibit the growth of Vp with the highest inhibition shown at the cocktail treatment (p<0.05). The survival of Artemia was higher in the phage treatments (p<0.005) compared to the infected control. Infected control had 68.33% of brine shrimp survival, and the Vb_Vp_TSK01, Vb_Vp_JKT01, and their cocktail had similar average brine shrimp survival of 91.11%. In conclusion, phage therapy proved effective in preventing vibriosis in brine shrimp under the conditions tested.
APLIKASI SINBIOTIK UNTUK PENCEGAHAN INFEKSI INFECTIOUS MYONECROSIS VIRUS PADA UDANG VANAME (Litopenaeus vannamei) (Synbiotic Application for Prevention of Infectious Myonecrosis Virus Infection in White Shrimp (Litopenaeus vannamei)) Widanarni, Widanarni; Sukenda, Sukenda; Septiani, Ghita Ryan
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5041

Abstract

This study aimed to evaluate the effectiveness of dietary synbiotic at different giving frequencies on growth, immune responses, and resistance of white shrimp infected by infectious myonecrosis virus (IMNV). Synbiotic used in this study was combination of probiotic Vibrio alginolyticus SKT-b and prebiotic oligosaccharides extracted from sweet potatoe (Ipomoea batatas L). Doses of probiotic and prebiotic used were 1% and 2% (w/w), respectively. The white shrimps (0.4930.035 g) were divided into five treatments consisting of A and B (without supplementation of synbiotic: (A) positive control; (B) negative control), C (daily synbiotic supplementation), D (twice a week synbiotic supplementation), and E (weekly synbiotic supplementation). After 30 days of feeding trial, white shrimps were infected by IMNV (except negative control). The results showed that daily growth rate of white shrimp on all synbiotic treatments (C, D, and E) ranged from 6.930.025-6.970.019% and had higher values than controls (A and B) (P0.05). Meanwhile, feed conversion value in C and D (1.540.142 and 1.580.117) were lower than controls (P0.05). Supplementation of synbiotic with different frequencies also affected survival rate of white shrimp after the challenge test with IMNV; daily synbiotic supplementation (C) resulted in a 50% higher survival rate than positive control (P0.05). This was associated with immune responses parameters values of synbiotic treatment (before and after the challenge test) which were better than positive control. In conclusion the addition of synbiotic in feed resulted in higher growth performances, immune responses,and resistance of white shrimp to IMNV infection.
Co-Authors , Rahman, , , Ranta, , , Rusli, , . ARIFUDDIN . Maryani . Rahman . Sunarto A. Hasan A. Santika A. Suwanto A.J. Sihombing Ade Dwi Sasanti Aditya, Tiya Widi Afif, Usamah Afiff , Usamah Aldy Mulyadin Alimuddin Alit Brilliant Aminatul Zahra Angela Mariana Lusiastuti ANGELA MARIANA LUSIASTUTI Anis Nugrahawati Annisa Astri Anggraeni Ayi Santika Bambang Gunadi Benny Yustim Brite, Margie Cahyawati, Nadia Catur A. Pebrianto D. Dana D. Meha D. Wahjuningrum Daniel Djokosetiyanto DEBY YUNIASARI Dendi Hidayatullah, Dendi Dewi Rahmi DIAH AYU SATYARI UTAMI Dian Febriani Dinamella Wahjuningrum Dwi Agung Saputra E. Ayuzar E. Harris Eddy Supriyono Eka Angga Laksana Enang Harris Enang Harris Esti Handayani Hardi Evan Farhan Wahyu Puadi F.H. Pasaribu Fachriyan Hasmi Pasaribu Fadilah, Iin Nur Fadlilah, Rizqy Aditya Fitria Novianti Gustilatov, Muhamad Helmy Faisal Muttaqin Henky Manoppo Hisatsugu Wakabayashi I. Normalina I. Nur Iqbal Kurniawinata, Mohamad Irzal Effendi Isni Rahmatika Sari Jasmanindar, Yudiana Jeanni Indah Noermala Julie Ekasari K. Sumantadinata Khairun Nissa Komar Sumantadinata Kukuh Nirmala L. Jamal Lastriliah, Mira LAY, B. W. M. Yuhana M. Zairin Junior M.S. Arifin Mia Setiawati Mochamad Fatuchri Sukadi Muchtar, Muthahharah Muh. Aris Muh. Fatuhcri Sukadi Muharram Nur Ikhsan Mulyani, Rahma MUNTI YUHANA N.A. Maswan Nasri Julaini Nasrullah, Hasan Nur Bambang Priyo Utomo Nuzullia, Laely Odang Carman Ode, Inem P. Hadi Pras, Eva Prasetiyono Pratiwi, Kiki Amalia Putri, Shofii Amaliah R.D. Soejoedono Rafsyanzani, Muhammad Mufthi Rahman Rahman Ramadhani, Dian Eka Ramhirez, Putri Shandra Retno Damayanti Soejoedono Ririn Nurul Fauziah, Ririn Nurul Rizki Praseto, Rizki Rr. Bellya Anasti Maharani Rudi, Mad Ruku Ratu Borut Samsu Adi Rahman Sari Anggraeni, Sukma Septiani, Ghita Ryan Sri Nuryati Sri Nuryati Sugeng Budiharsono Sugiyo Hadi Pranoto Tatag Budiardi Trian Rizky Febriansyah Tsani Untsa, Agista Uba Umbara Ulil Surtia Zulpratita Umbara, Uba Wesly Pasaribu WIDANARNI WIDANARNI Wisriati Lasima Y. Tri Anggoro Yan Evan Yumaidawati, Nurfitriani Siti Yuni Puji Hastuti Zulfani, Anisa