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The health status of hybrid grouper Epinephelus fuscoguttatus (♀) x E.lanceolatus (♂) cultured in floating-net cage at Kelapa Dua Island, Seribu Islands, Indonesia Ratu Borut, Ruku; Sukenda; Sri Nuryati; kukuh Nirmala; Irzal Effendi; Widanarni
Jurnal Akuakultur Indonesia Vol. 22 No. 2 (2023): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.22.2.106-114

Abstract

This study aimed to identify the fish health status, based on the total erythrocytes (cells/mm3), total leukocytes (cells/mm3), hemoglobin level (g %), hematocrit, and histopathological profiles. This study was performed on February, 2021 – January, 2022. The study was located in floating-net cage of Kelapa Dua Island, Seribu Islands, Jakarta, Indonesia. Sampling was performed by a purposive-sampling method, environmental parameter measurements, containing temperature and salinity, clinical symptom observation in fish, total erythrocytes, leukocytes, hemoglobin, hematocrit, histopathological profiles of hybrid grouper liver and kidney, and existence of VNN virus. The observation results present the behavior changes in sick fish, namely whirling on the net cage surface, dead-sleeping or death-like condition below the net cage. Hybrid grouper has total erythrocytes of 1.36 x 106 – 5.13 x 106 cells/mm3, total leukocytes of 2.9 x 104 – 7.9 x 104 cells/mm3, hemoglobin level of 5.56 - 10.94 g %, and hematocrit of 13.31 - 24.78 %. From the histopathological observation, hepatocyte, necrosis, vacuolysis was occurred in liver and tubular, leukocyte infiltasi, nicrosis was occurred in kidney and with the highest prevalence of VNN at 20 – 80 %. Therefore, hybrid grouper is sick due to increased number of leukocytes at 5.13 x 106 cells/mm3 above the normal range, followed by decreased hemoglobin and hematocrit levels at 5.56 - 10.94 g % and 13.31 - 24.78 %, respectively, due to organ damage. Keyword: must be written in not more than 5 words and alphabeticaly listed ABSTRAK Penelitian ini bertujuan untuk mengetahui status kesehatan ikan meliputi total eritrosit (sel/mm3), total leukosit (sel/mm3), kadar hemoglobin (gram %) dan hematokrit (%) dan gambaran histopatolgi. Penelitian ini dilaksanakan pada periode bulan Februari 2021 hingga Januari 2022. Lokasi penelitian pada keramba jaring apung (KJA) di Pulau Kelapa Dua Kepulauan Seribu. Metode pengambilan sampel ikan dilakukan secara purposive sampling, pengukuran parameter lingkungan perairan yaitu suhu dan salinitas, pengamatan gajala klinis ikan, perhitungan jumlah eritrosit, leukosit, kadar hemoglobin dan hematokrit serta gambaran histopatologi pada organ hati dan ginjal ikan kerapu cantang dan keberadaan virus VNN. Hasil pengamatan menunjukkan perubahan tingkah laku ikan sakit yaitu berputar ke atas permukaan KJA, terjadi sleeping dead atau ikan berada di dasar seperti mati. Ikan kerapu cantang memiliki jumlah eritrosit 1.36 x 106 – 5.13 x 106 sel/mm3, leukosit 2.9 x 104 – 7.9 x 104 sel/mm3, hemoglobin 5.56 - 10.94 gr %, dan hematokrit 13.31 - 24.78 %, hasil histopalogi ikan mengalami perubahan sel pada hati yaitu hepatosit, vakuolisasi, nekrosis dan ginjal mengalami perubahan infiltrasi leukosit, nicrosis, tubulus dan kehadiran virus VNN tertinggi berkisar 20 % - 80 %. Berdasarkan hasil penelitian tersebut maka dapat disimpulkan bahwa ikan kerapu cantang yang sakit karena jumlah leukosit meningkat 5.13 x 106 sel/mm3 melebihi kisaran normal, kadar hemoglobin menurun 5.56 - 10.94 % dan kadar hematokrit 13.31 - 24.78 % rendah dan terjadi kerusakan jaringan pada organ dalam ikan. Kata kunci: Ikan Kerapu Cantang, Hematology, Histopatologi, Parameter Fisika dan Kimia
Evaluation of single and multispecies probiotic applications for the prevention of Motile Aeromonads Septicaemia in gourami, Osphronemus gourami Tsani Untsa, Agista; Widanarni, Widanarni; Sukenda, Sukenda; Afiff, Usamah; Yuhana, Munti
Jurnal Akuakultur Indonesia Vol. 23 No. 1 (2024): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.23.1.56-70

Abstract

The aim of this study was to evaluate single and multispecies probiotic applications for the prevention of Motile Aeromonads Septicaemia (MAS) disease in gourami, Osphronemus gourami. The experiment consisted of the in vitro inhibition test and the in vivo application of probiotics in gourami. The in vivo assay, consisted of five treatments and five replicates, namely: negative control (K-); positive control (K+); (B) fish fed with supplementation of 1% (v/w) probiotic Bacillus NP5 RifR cells and challenged with Aeromonas hydrophila RifR; (L) fish fed with supplemention of probiotic Lactobacillus plantarum CipR 1% (v/w); challenged with A. hydrophila RifR; (BL) fish fed with mixture supplementation of Bacillus NP5 RifR 0.5% (v/w)+L. plantarum CipR 0.5%; infected with A. hydrophila RifR. Fish (29.57 ± 1.00 g) were reared in a 45 L volume aquaria with a rearing density of 10 fish/m3 for 50 days, with feeding trial three times a day. The challenge test was conducted by intramuscular injection with pathogenic A. hydrophila RifR cells (106 CFU/mL) on day 41st. In vitro test results showed that single and multispecies probiotics significantly (P<0.05) inhibited A.hydrophila RifR. In vivo experiment showed that probiotic supplementation treatments improved the growth performance, and microbiota diversity in the gut. The immune responses, fish resistance to A. hydrophila RifR and gourami survival rate in all treatments of supplemented feed were significantly higher compared to the positive control. The best treatment, multispecies probiotics significantly (P<0.05) improved the survival of gourami 96.67% post infection with A. hydrophila RifR. Keywords: A. hydrophila, Bacillus NP5, L. plantarum, O. gourami, probiotic ABSTRAK Penelitian ini bertujuan untuk mengevaluasi aplikasi probiotik tunggal dan multispesies dalam pencegahan penyakit Motile Aeromonad Septicaemia (MAS) yang disebabkan oleh Aeromonas hydrophila pada ikan gurami (Osphronemus gourami). Penelitian terdiri atas uji penghambatan in vitro bakteri probiotik tunggal dan multispesies terhadap A. hydrophila RifR, dan uji in vivo aplikasi probiotik untuk gurami. Uji in vivo, terdiri dari lima perlakuan dan lima ulangan, yaitu: kontrol negatif (K-) kontrol positif (K+); (B) ikan dengan pakan suplementasi Bacillus NP5 RifR 1% dan diinfeksi A. hydrophila RifR; (L) pakan komersial dengan penambahan probiotik L. plantarum CipR 1% dan infeksi A. hydrophila RifR; (BL) ikan dengan suplementasi campuran Bacillus NP5 RifR 0.5% dan L. plantarum CipR 0.5% serta diinfeksi A. hydrophila RifR. Benih gurami (29.57 ± 1.00 g) dipelihara di dalam akuarium bervolume 45 L dengan kepadatan 10 ekor/m3 selama 50 hari, dengan pakan bersuplemen probiotik 3 kali sehari. Uji tantang dilakukan dengan menginjeksikan suspensi sel patogen A. hydrophila RifR (106 CFU/mL) secara intramuscular pada hari ke 41. Hasil uji in vitro menunjukkan bahwa probiotik tunggal dan multispesies dapat menghambat pertumbuhan sel A.hydrophila RifR di organ hati dan ginjal secara signifikan (P<0.05). Hasil uji in vivo menunjukkan perlakuan aplikasi probiotik tunggal dan multispesies mampu meningkatkan kinerja pertumbuhan, keragaman mikrobiota usus. Respons imunitas, resistansi terhadap A. hydrophila RifR dan kelangsungan hidup gurami di semua perlakuan pakan bersuplemen probiotik secara signifikan lebih tinggi dibandingkan kontrol positif. Perlakuan terbaik adalah probiotik multispesies meningkatkan kelangsungan hidup ikan sebesar 96.67% secara signifikan (P<0.05) pascainfeksi A. hydrophila RifR. Kata kunci: A. hydrophila, Bacillus sp. NP5, L. plantarum, O. goramy, probiotik
The presence of disease in growing spiny lobster Panulirus spp. in floating net cages at Tanjung Putus Island, Lampung Sukenda; Lastriliah, Mira; Widanarni, Widanarni; Effendi, Irzal; Hidayatullah, Dendi
Jurnal Akuakultur Indonesia Vol. 23 No. 1 (2024): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.23.1.24-35

Abstract

Spiny lobster is a member of crustaceans that has high economic value. The demand for export markets in Asian, European and American countries as well as locally, is quite high with an expensive selling price. However, there are obstacles that often occur in spiny lobster rearing cultivation, namely infectious diseases that will cause high mortality. The purpose of this study was to investigate infectious diseases in rearing spiny lobster Panulirus spp. that were kept in floating net cages at Tanjung Putus, Lampung. The research method involved sampling five spiny lobsters in floating net cages, observation of clinical symptoms, bacterial isolation, identification of bacteria, detection of Milky Hemolymph Disease of Spiny Lobster (MHD-SL) through polymerase chain reaction (PCR) analysis and histopathology. The sampling location was at PT. Saibatin Perikanan Indonesia. The bacteria Vibrio parahaemolyticus and Vibrio alginolyticus were detected. Five lobsters were infected by MHD-SL, characterized by milky white hemolymph. All five lobster samples were positively infected with Milky Hemolymph Disease of Spiny Lobster (MHD-SL) through PCR analysis. Histopathological observations showed pathological microanatomical changes in the lobster hepatopancreas tissue, indicating that the tissue changes, in the form of encapsulation and infiltration were due to Rickettsia-like bacteria (RLB) infection. The management of spiny lobster stocking density, maintenance biosecurity, and increased lobster immunity must be implemented in order to prevent MHD-SL disease in floating net cages. Keywords: bacteria, histopathology, MHD-SL, Panulirus spp. ABSTRAK Lobster merupakan anggota crustacea yang memiliki nilai ekonomis yang tinggi. Permintaan pasar ekspor pada negara Asia, Eropa, dan Amerika maupun lokal cukup tinggi dengan nilai jual yang mahal. Akan tetapi, terdapat kendala yang sering terjadi pada budidaya pembesaran lobster yaitu infeksi penyakit yang akan menyebabkan tingginya mortalitas Tujuan penelitian ini adalah untuk menginvestigasi penyakit infeksi pada pembesaran lobster Panulirus spp. yang dipelihara di keramba jaring apung Tanjung Putus, Lampung. Metode penelitian ini meliputi pengambilan sampel lobster sebanyak lima ekor dalam karamba jarring apung (KJA), pengamatan gejala klinis, isolasi bakteri, identifikasi bakteri, deteksi Milky Hemolymph Disease of Spiny Lobster (MHD-SL) melalui analisis Polymerase Chain Reaction (PCR), dan histopatologi. Lokasi pengambilan sampel di PT. Saibatin Perikanan Indonesia, Lampung. Terdapat bakteri Vibrio parahaemolyticus dan V. alginolyticus. Lima ekor lobster terinfeksi oleh MHD-SL yang ditandai dengan hemolim berwarna putih susu. Lima sampel lobster positif terinfeksi penyakit Milky Hemolymph Disease of Spiny Lobster (MHD-SL) melalui analisis PCR. Hasil pengamatan histopatologi terdapat perubahan mikroanatomi patologi pada jaringan hepatopankreas lobster menunjukkan bahwa adanya perubahan jaringan berupa enkapsulasi dan infiltrasi karena adanya infeksi Rickettsia-like bacteria (RLB). Pengelolaan kepadatan penebaran lobster, biosekuritas pemeliharaan, dan peningkatan imunitas lobster harus ditingkatkan untuk mencegah penyakit MHD-SL di KJA. Kata kunci: bakteri, histopatologi, MHD-SL, Panulirus spp.
Distribution of Vibrio parahaemolyticus in pacific white shrimp Litopenaeus vannamei through immersion as a natural infection model Sari Anggraeni, Sukma; Sukenda; Nuryati, Sri; Wahjuningrum, Dinamella
Jurnal Akuakultur Indonesia Vol. 23 No. 2 (2024): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.23.2.176-188

Abstract

Vibrio parahaemolyticus is a pathogenic bacterium that indusces mortality is pacific white shrimp. This study aimed to analyze the distribution of V. parahaeomyticus in shrimp through immersion and evaluate, polymerase chain reaction, histopathology, and immune response. The study employed two treatments with four replicates each. Shrimp were infected through immersion with V. parahaemolyticus at a concentration of 107 CFU/mL for 30 minutes, followed by transferring the shrimp to a rearing container. Gill, hepatopancreas, gut, and hemolymph samples were collected at 6, 12, and 24 hours post-infection. Infected shrimp exhibited clinical symptoms such as a pale body and hepatopancreas, along with empty gut. Shrimp mortality reached 97.08%, while no mortality occurred in the control grouph. The abundance of V. parahaemolyticus in organs exceeded 105 CFU/mL. Histopathological examination revealed mild, moderate, and severe damage. PCR confirmation tests at 12 and 24 hours yielded positive results, with a DNA band at 230 bp. Immune responses, including total hemocytes, phagocytic activity, phenoloxidase, and respiratory burst, demonstrated significantly different results (P<0.05). It was concluded that the distribution of V. parahaemolyticus in the shrimp body, specifically in the gills, hepatopancreas, and gut, exceed 105 CFU/mL, as confirmed by a 230 bp DNA band in the PCR test. Tissue damage, manifested as lesions and necrosis, occurred in the gills, hepatopancreas, and gut. Additionally, the imun response of Vibrio-infected shrimp was found to be lower than that of uninfected shrimp (negative control). Keywords: distribution, immersion, Litopenaeus vannamei, Vibrio parahaemolyticus ABSTRAK Vibrio parahaemolyticus adalah bakteri patogen yang menyebabkan kematian pada udang vaname. Penelitian ini bertujuan menganalisis distribusi V. parahaemolyticus pada udang melalui perendaman serta kerusakan yang ditimbulkan melalui pengamatan gejala klinis, angka lempeng total bakteri, polymerase chain reaction, histopatologi dan respons imun. Penelitian menggunakan dua perlakuan dan empat ulangan. Udang diinfeksi melalui perendaman menggunakan V. parahaemolyticus 107 CFU/mL selama 30 menit, kemudian udang dipindah pada wadah pemeliharaan. Pengambilan organ insang, hepatopankreas, usus dan hemolim dilakukan pada jam ke-6, 12, dan 24 pascainfeksi. Udang terinfeksi memiliki gejala klinis seperti tubuh dan hepatopankreas pucat, usus kosong. Mortalitas udang mencapai 97.08% dan tidak ada kematian pada udang kontrol. Kelimpahan V. parahaemolyticus pada organ lebih dari 105 CFU/mL. Pengamatan histopatologi menunjukkan kerusakan ringan, sedang, hingga parah. Uji konfirmasi PCR jam ke-12 dan ke-24 menunjukkan hasil positif pada 230 bp. Respons imun seperti total hemosit, aktivitas fagositik, phenoloxidase, respiratory burst menujukkan hasil yang berbeda nyata (P<0.05). Disimpulkan bahwa distribusi V. parahaemolyticus di dalam tubuh udang pada terdapat pada insang, hepatopankreas dan usus lebih dari 105 CFU/mL dan terkonfirmasi uji PCR pada pita DNA 230 bp. Kerusakan jaringan seperti lesi dan nekrosis terjadi pada insang, hepatopankreas, dan usus. Respons imun udang yang terinfeksi Vibrio lebih rendah dibandingkan udang yang tidak terinfeksi (kontrol negatif). Kata kunci: distribusi, Litopenaeus vannamei, perendaman, Vibrio parahaemolyticus
Potential of Eurycoma longifolia, Curcuma zedoaria, and Allium sativum extracts as phytobiotics for shrimp health Iqbal Kurniawinata, Mohamad; Sukenda, Sukenda; Wahjuningrum, Dinamella; Widanarni, Widanarni; Ekasari, Julie
Jurnal Akuakultur Indonesia Vol. 24 No. 1 (2025): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.24.1.71-81

Abstract

This study evaluated the efficacy of Eurycoma longifolia, Curcuma zedoaria, and Allium sativum in improving the immune response and resistance of whiteleg shrimp to prevent Vibrio parahaemolyticus infection. The study consisted of two phases, an in vitro phase to determine the compounds contained in three medicinal plants as antibacterials, followed by an in vivo phase to evaluate the effect of the medical plant extract on immune response and robustness against V. parahaemolyticus. The results from the first phase revealed that bioactive compounds present in E. longifolia were more varied and had higher concentrations with a lower bactericidal value when compared to those found in C. zedoaria or A. sativum. In the second phase of the experiment, the medicinal plant extract was added to the feed with a dose that was determined according to the first phase results. The treatments tested in the second phase were 1.6% E. longifolia extract dietary addition (EL16), 6.4% C. zedoaria extract dietary addition (CZ64), 6.4% A. sativum extract dietary addition (AS64) and phytobiotics mixture of 1:1:1 (C1) dietary addition, as well as no phytobiotic for negative control treatment and positive control. The results from the second stage demonstrated that dietary phytobiotic extract addition enhances the immunological responses and improves the shrimp survival against V. parahaemolyticus challenge compared to the control group. In conclusion, E. longifolia, C. zedoaria, and A. sativum showed different bioactive compound profiles, which affect their efficacy against V. parahaemolyticus, with EL16 showing higher efficacy. Keywords: A. sativum, C. zedoaria, E. longifolia, Penaeus vannamei, Phytobiotic ABSTRAK Penelitian ini mengevaluasi efikasi Eurycoma longifolia, Curcuma zedoaria, dan Allium sativum dalam meningkatkan respon imun dan resistensi udang vaname untuk mencegah infeksi Vibrio parahaemolyticus. Penelitian ini terdiri dari dua tahap: tahap in vitro untuk menentukan senyawa yang terkandung dalam tiga tanaman obat sebagai antibakteri, diikuti oleh tahap in vivo untuk mengevaluasi efek ekstrak tanaman obat terhadap respon imun dan ketahanan terhadap V. parahaemolyticus. Hasil dari tahap pertama mengungkapkan bahwa senyawa bioaktif yang ada dalam E. longifolia lebih bervariasi dan memiliki konsentrasi yang lebih tinggi dengan nilai bakterisida yang lebih rendah jika dibandingkan dengan yang ditemukan di C. zedoaria atau A. sativum. Pada percobaan tahap kedua, ekstrak tanaman obat ditambahkan ke pakan dengan dosis yang ditentukan sesuai dengan hasil tahap pertama. Perlakuan yang diuji pada tahap kedua adalah penambahan 1,6% ekstrak E. longifolia dalam pakan (EL16), penambahan 6,4% ekstrak C. zedoaria dalam pakan (CZ64), penambahan 6,4% ekstrak A. sativum dalam pakan (AS64) dan campuran fitobiotik 1:1:1 (C1) dalam pakan, serta tanpa fitobiotik untuk perlakuan kontrol negatif dan kontrol positif. Hasil dari tahap kedua menunjukkan bahwa penambahan ekstrak fitobiotik dalam pakan meningkatkan respons imunologi dan memperbaiki kelangsungan hidup udang terhadap tantangan V. parahaemolyticus dibandingkan dengan kelompok kontrol. Sebagai kesimpulan, E. longifolia, C. zedoaria, dan A. sativum menunjukkan profil senyawa bioaktif yang berbeda, yang mempengaruhi efikasinya terhadap V. parahaemolyticus, dengan EL16 menunjukkan efikasi yang lebih tinggi. Kata kunci: Allium sativum, Curcuma zedoaria, Eurycoma. longifolia, fitobiotik, Penaeus vannamei
Meningkatkan kemampuan pemahaman siswa dengan menggunakan metoda penemuan terbimbing pada pembelajaran geometri kelas 8 Sukenda Sukenda; Uba Umbara; Evan Farhan Wahyu Puadi
JUMLAHKU: Jurnal Matematika Ilmiah STKIP Muhammadiyah Kuningan Vol 4 No 1 (2018): Edisi Vol. 4 No. 1 Mei
Publisher : Program Studi Pendidikan Matematika STKIP Muhammadiyah Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The purpose of the study was to investigate whether Guided Finding Methods could improve students' understanding of geometry in class 8. The method used is Classroom Action Research. The research was done at SMP Negeri 2 Ciwaru located at Jl. Raya Sumberjaya, Desa Citikur, Kecamatan Ciwaru, Kabupaten Kuningan. The subjects of the researchs were the students of SMP Negeri 2 Ciwaru class 8 academic year 2016/2017. They were chosen because after consulting with mathematics teachers that this had major problems regarding the comprehension ability of mathematical concepts. Data collection techniques: observation, interview and questionnaire. The research was done in 2 cycles, each cycle was done in 3 sessions. Each session includes 4 stages: planning, action, observation and reflection. The results of the research as follows: Learning with guided discovery method can improve students' understanding 73.82%. With the following details on the first cycle students who reached the KKM 17 students (62.54%) with an average score of 65.74. In cycle II the number of KKM 23 students (85.19%) the average score in cycle II was 79.26. Based on the results of the research it is advisable to use guided discovery methods in improving students' comprehension skills.
Making Learning Module Using Google Spaces for Kindergarten Teachers in Pangandaran Based on LMS (Learning Management System) Wahyu Wibowo, Ari Purno; Laksana, Eka Angga; Sukenda, Sukenda; Yustim, Benny
Brilliance: Research of Artificial Intelligence Vol. 5 No. 1 (2025): Brilliance: Research of Artificial Intelligence, Article Research May 2025
Publisher : Yayasan Cita Cendekiawan Al Khwarizmi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47709/brilliance.v5i1.5770

Abstract

Learning process Children of kindergarten age tend to get bored easily and must use a different approach, because at an early age children will get tired easily if taught something tire Computers with attractive visual displays, animations, and sounds can make the learning process feel like playing, so that they are more enthusiastic and motivated. to overcome this, it is necessary to create a more innovative multimedia-based teaching system on the material taught must have parameters that are visual, audio and interactive material, so that the material delivered can be easily measured according to learning outcomes, in this study the author tried to use the help of open source applications based on Google Spacess and LMS (Learning Management System) which can be used by kindergarten teachers in the Pangandaran area, by using this Google Spaces assistance, it turns out that teachers can create the same and more interactive teaching material, in the end with the help of digital media, the material can be adjusted to the individual abilities of each child's speed and can be evaluated directly because for example children who have mastered the material can proceed to the next stage, while on the contrary students who are left behind in learning can repeat more relaxedly repeating the learning, then with the help of teachers and the student learning process can be done independently and not limited by time, because the LMS module can be accessed at any time by students. From the results of the training held for 65 kindergarten teachers, 85% agreed that this LMS application was very helpful in teaching and easy to configure and re-develop.
Kebijakan Inovatif untuk Akuakultur Berkelanjutan: Optimalisasi Peran Bivalvia dalam Pengolahan Limbah Pras, Eva Prasetiyono; Nirmala, Kukuh; Supriyono, Eddy; Sukenda; Hastuti, Yuni Puji
Policy Brief Pertanian, Kelautan, dan Biosains Tropika Vol. 7 No. 2 (2025): Policy Brief Pertanian, Kelautan, dan Biosains Tropika
Publisher : Direktorat Kajian Strategis dan Reputasi Akademik IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agro-maritim.0702.1272-1276

Abstract

Pertumbuhan industri tambak udang intensif di Indonesia telah memberikan kontribusi ekonomi yang signifikan. Namun tambak udang juga menimbulkan permasalahan lingkungan akibat buangan kaya nutrien seperti amonium, nitrat, ortofosfat, partikel organik dan tersuspensi. Kandungan nutrien yang tinggi dalam buangan tambak dapat memicu ledakan populasi mikroalga, menurunkan kualitas lingkungan dan terganggunya keseimbangan ekosistem perairan. Pemerintah telah mengeluarkan kebijakan yang mewajibkan setiap tambak udang memiliki unit pengolahan limbah sebagai bentuk pengendalian dampak lingkungan. Namun, implementasi kebijakan ini menghadapi tantangan besar terkait dengan besarnya biaya investasi dan operasional instalasi pengolahan limbah tersebut. Upaya untuk memberikan solusi alternatif yang lebih efisien dan ramah lingkungan harus dilakukan, diantaranya dengan menggunakan bivalvia. Pemanfaatan bivalvia— seperti kerang darah—dapat menjadi pendekatan biotreatment yang efektif. Bivalvia berperan sebagai filter feeder yang mampu mengasimilasi mikroalga dan partikel nutrien di dalam air. Filtrasi menggunakan kerang darah terbukti mampu menurunkan konsentrasi mikroalga dan nutrien, masing-masing sebesar 72,80% untuk mikroalga, 36,78% untuk amonia, 50,00% untuk nitrit, 78,77% untuk nitrat, dan 89,13% untuk ortofosfat.. Rekomendasi alternatif kebijakan diarahkan pada integrasi penggunaan bivalvia dalam sistem pengolahan limbah tambak, dan penyusunan pedoman teknis biotreatment yang lebih efisien sebagai bagian dari strategi pengelolaan limbah berkelanjutan. Strategi ini tidak hanya memperkuat upaya perlindungan lingkungan, tetapi juga membuka peluang ekonomi tambahan melalui produksi bivalvia sebagai komoditas konsumsi.
Cyber Pandemic – The New Cybersecurity Risks Sukenda, Sukenda; Zulpratita, Ulil Surtia; Muttaqin, Helmy Faisal; Wahyu, Ari Purno; Yustim, Benny
Brilliance: Research of Artificial Intelligence Vol. 4 No. 2 (2024): Brilliance: Research of Artificial Intelligence, Article Research November 2024
Publisher : Yayasan Cita Cendekiawan Al Khwarizmi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47709/brilliance.v4i2.4759

Abstract

We are amidst a digital pandemic. In 2020, COVID-19 sped up a change towards remote working and the product being utilized for these assaults has become more straightforward to execute, ransom ware assaults have risen quickly and keep on speeding up in 2021. The COVID-19 pandemic has changed both the actual world and the computerized space, where organizations and associations are being gone up against with overwhelming online protection challenges for which not many were prepared or prepared to confront. Attributable to the extreme change in working conditions, cyber attacks and information extortion presently rank third among the best worries of business pioneers, as detailed in the World Economic Forum's COVID-19 Risks Outlook. The likelihood of malignant digital movement is considerably more upsetting thinking about that 53% of organizations have never pressure tried their systems. The key focus point from these and a large group of other alarming probabilities is that readiness for any kind of digital emergency whatsoever levels of an association is critical. Top administration, network protection trained professionals and each representative should know how to treat an emergency hit. Cyber security centers around securing information, yet it is presently not adequate; organizations need cyber resilience.
Isolation, Characterization, and In Vitro Evaluation of Bacteriophages for Controlling the Fish Pathogen Aeromonas hydrophila Nasri Julaini; Wahjuningrum, Dinamella; Widanarni; Sukenda
Jurnal Ilmiah Perikanan dan Kelautan Vol. 18 No. 1 (2026): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v18i1.79707

Abstract

Graphical Abstract   Highlight Research 1. A lytic bacteriophage was successfully isolated from catfish pond water in Dramaga, Bogor, with a high density of 5.68 × 10⁹ PFU/mL. 2. Characterization revealed round to irregular plaque morphology with high clarity and diameters reaching 0.35 cm. 3. The bacteriophage exhibited high host specificity, being effective only against the A. hydrophila AH03 isolate from the AAHL collection. 4. Treatment at MOI 10 resulted in more effective inhibition of A. hydrophila growth compared to other treatments.   Abstract Aquaculture plays a vital role in global protein supply, yet its increasing production faces disease-related challenges, particularly A. hydrophila infections. This pathogen causes Motile Aeromonas Septicemia (MAS), leading to mass mortality in catfish and significant economic losses. While antibiotics have been the primary solution, their use is now restricted due to antimicrobial resistance, necessitating safe and sustainable alternatives. Phage have emerged as environmentally friendly, specific biocontrol agents to combat such infections. This study aimed to evaluate the effectiveness of lytic phage in controlling the fish pathogen A. hydrophila through an in vitro approach. The research stages included phage isolation (density assay), characterization (plaque morphology and host range testing), and evaluation of phage against A. hydrophila (bacteriolytic efficacy assay). A completely randomized design was employed, with six treatments and three replicates, media control (K-), A. hydrophila control (K+), antibiotic control (enrofloxacin 0.1 mg/mL, KA), and phage treatments at multiplicities of infection (MOI) of 0.1, 1, and 10 (P0.1, P1, P10) for the bacteriolytic efficacy assay. Phage isolated from catfish ponds in Dramaga, Bogor, exhibited a high titer (5.68 × 10⁹ PFU/mL) and formed clear, round plaques (0.17-0.35 cm diameter). They demonstrated a narrow host range, lysing only 1 of 10 A. hydrophila isolates. In efficacy assays, phage treatment at an MOI of 10 significantly reduced bacterial density (p < 0.05) at 24 hours post infection, decreasing the OD 600 nm by 38.7% compared to the positive control. These results indicate the potential of these phage as an antibiotic alternative for controlling A. hydrophila in aquaculture.    
Co-Authors , Rahman, , , Ranta, , , Rusli, , . ARIFUDDIN . Maryani . Rahman . Sunarto A. Hasan A. Santika A. Suwanto A.J. Sihombing Ade Dwi Sasanti Aditya, Tiya Widi Afif, Usamah Afiff , Usamah Aldy Mulyadin Alimuddin Alit Brilliant Aminatul Zahra ANGELA MARIANA LUSIASTUTI Angela Mariana Lusiastuti Anis Nugrahawati Annisa Astri Anggraeni Ayi Santika Bambang Gunadi Benny Yustim Brite, Margie Cahyawati, Nadia Catur A. Pebrianto D. Dana D. Meha D. Wahjuningrum Daniel Djokosetiyanto DEBY YUNIASARI Dendi Hidayatullah, Dendi Dewi Rahmi DIAH AYU SATYARI UTAMI Dian Febriani Dinamella Wahjuningrum Dwi Agung Saputra E. Ayuzar E. Harris Eddy Supriyono Eka Angga Laksana Enang Harris Enang Harris Esti Handayani Hardi Evan Farhan Wahyu Puadi F.H. Pasaribu Fachriyan Hasmi Pasaribu Fadilah, Iin Nur Fadlilah, Rizqy Aditya Fitria Novianti Gustilatov, Muhamad Henky Manoppo Hisatsugu Wakabayashi I. Normalina I. Nur Iqbal Kurniawinata, Mohamad Irzal Effendi Isni Rahmatika Sari Jasmanindar, Yudiana Jeanni Indah Noermala Julie Ekasari K. Sumantadinata Khairun Nissa Komar Sumantadinata Kukuh Nirmala L. Jamal Lastriliah, Mira M. Yuhana M. Zairin Junior M.S. Arifin Mia Setiawati Mochamad Fatuchri Sukadi Muchtar, Muthahharah Muh. Aris Muh. Fatuhcri Sukadi Muharram Nur Ikhsan Mulyani, Rahma MUNTI YUHANA Muttaqin, Helmy Faisal N.A. Maswan Nasri Julaini Nasrullah, Hasan Nur Bambang Priyo Utomo Nuzullia, Laely Odang Carman Ode, Inem P. Hadi Pras, Eva Prasetiyono Pratiwi, Kiki Amalia Putri, Shofii Amaliah R.D. Soejoedono Rafsyanzani, Muhammad Mufthi Rahman Rahman Ramadhani, Dian Eka Ramhirez, Putri Shandra Retno Damayanti Soejoedono Ririn Nurul Fauziah, Ririn Nurul Rizki Praseto, Rizki Rr. Bellya Anasti Maharani Rudi, Mad Ruku Ratu Borut Samsu Adi Rahman Sari Anggraeni, Sukma Septiani, Ghita Ryan Sri Nuryati Sri Nuryati Sugeng Budiharsono Sugiyo Hadi Pranoto Tatag Budiardi Trian Rizky Febriansyah Tsani Untsa, Agista Uba Umbara Ulil Surtia Zulpratita Umbara, Uba Wesly Pasaribu WIDANARNI WIDANARNI Wisriati Lasima Y. Tri Anggoro Yan Evan Yumaidawati, Nurfitriani Siti Yuni Puji Hastuti Zulfani, Anisa