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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Akuakultur Indonesia Jurnal Veteriner e-Journal BUDIDAYA PERAIRAN Jurnal Natur Indonesia Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) OCTOPUS : Jurnal Ilmu Perikanan Jurnal Ilmiah KOMPUTASI Omni-Akuatika Indonesian Aquaculture Journal JUMLAHKU: Jurnal Matematika Ilmiah STKIP Muhammadiyah Kuningan Brilliance: Research of Artificial Intelligence Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Jurnal Algoritma Jurnal Kedokteran Hewan Strata Law Review Policy Brief Pertanian, Kelautan, dan Biosains Tropika Journal of Fisheries & Marine Jurnal Matematika Ilmiah Universitas Muhammadiyah Kuningan

Sukenda .

Departemen Budidaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan, Institut Pertanian Bogor Kampus IPB Darmaga Bogor 16680

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Screening of Probiotic Bacteria from Intestine and Culture Environment of Hoeven’s slender carp Leptobarbus hoeveni Blkr to Control Pathogenic Bacteria Sunarto, .; Sukenda, .; Widanarni, .
Jurnal Akuakultur Indonesia Vol. 9 No. 2 (2010): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The ability of probiotic bacteria to control disease infection has been used in aquaculture. This experiment was conducted to isolate and characterize probiotic bacteria; the competition test its ability probiotic bacteria against pathogenic bacteria; and to improve survival rate of Leptobarbus hoeveni. The bacteria were isolated from Leptobarbus hoeveni and its culture environment, and then tested to know its ability to inhibit bacterial fish pathogen in-vitro. Furthermore, the selected probiotic bacteria were tested in vivo to evaluate their ability to inhibit pathogen of Leptobarbus hoeveni. The result showed that probiotic bacteria inhibit the growth of Streptococcus iniae, Flexibacter columnaris, Mycobacterium fortuitum and Aeromonas hydrophila in vitro. Isolate DD3 was the best of candidate probiotic because of the ability to inhibit pathogen, especially A. hydrophila, the most virulent bacteria in Leptobarbus hoeveni.Key Words : probiotic bacteria, Leptobarbus hoeveni, pathogenic bacteriaAbstrakKemampuan bakteri probiotik untuk mengendalikan penyakit infeksi telah digunakan dalam akuakultur. Tujuan penelitian ini adalah mengisolasi dan mengkarakterisasi bakteri probiotik, menguji kemampuan bakteri probiotik terhadap bakteri patogen, sehingga dapat meningkatkan tingkat kelangsungan hidup ikan jelawat. Bakteri diisolasi dari usus ikan jelawat dan lingkungan budaya, kemudian diuji kemampuannya menghambat bakteri patogen secara in-vitro. Selanjutnya bakteri probiotik yang dipilih diuji secara in vivo untuk mengevaluasi kemampuannya dalam menghambat patogen di dalam tubuh ikan jelawat. Dari hasil penelitian diperoleh bakteri probiotik yang diisolasi dari usus dan lingkungan budaya ikan jelawat menunjukkan penghambatan pertumbuhan terhadap Streptococcus iniae, Flexibacter columnaris, Mycobacterium fortuitum dan Aeromonas hydrophila secara in vitro. Isolat DD3 merupakan kandidat probiotik terbaik, karena mempunyai kemampuan untuk menghambat bakteri patogen, khususnya bakteri A. hydrophila adalah bakteri yang paling viluren bagi ikan jelawat. Kata Kunci: bakteri probiotik, ikan jalawat dan baktri patogen

Isolation of probiotics bacterium from coral reef for controlling vibriosis in tiger shrimp (Penaeus monodon) larvae Sasanti, Ade Dwi; Widanarni, .; Sukenda, .
Jurnal Akuakultur Indonesia Vol. 9 No. 2 (2010): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Pathogenic Vibrio, especially luminous Vibrio harveyi, could cause mass mortality in tiger shrimp culture. One of the technique to work against luminous Vibrio is, using probiotic bacteria to inhibit the luminous Vibrio growth. This study was carried out to obtain bacteria isolates from coral reef which potentially inhibit V. harveyi growth. A total of 110 isolates were isolated from Acropora sp, Merulina sp, Hystrix sp., Poecillophora sp, Porites sp and Haliophora sp., and have probiotic activity against V. harveyi in in vitro and in vivo test.Of the total 110 isolates, 54 isolates show the inhibiting zone. Two isolates(8A and 1C)were not pathogenic and have the most effective activity in inhibiting growth of V. harveyi and significantly reduced larval mortality in in vitro and in vivo test. Treatment using probiotics candidate have significant different survival rate (83.33%) compared with positive control (61.67%). The growth rate of lenght of larvae treatment with isolate of 8A (5.25%) and 1C (5.06%) show the significant different compared with positive control (3.54%). The growth rate of weight of larvae treatment withisolate of 8A (17.51%) and 1C (17.61%) show significant different compared with negative (15.27%) and positive control (14.69%).Key word: coral reef, probiotic, tiger shrimp, vibriosis, V. harveyi. ABSTRAKVibrio patogen, khususnya Vibrio harveyi berpendar, dapat menyebabkan kematian massal pada budidaya udang windu. Salah satu alternatif untuk menghambat Vibrio harveyi berpendar adalah dengan menggunakan bakteri probiotik yang dapat menekan pertumbuhan Vibrio tersebut. Penelitian ini bertujuan untuk mendapatkan isolat bakteri dari terumbu karang yang potensial menghambat pertumbuhan V. harveyi. Total 110 isolat diisolasi dari Acropora sp, Merulina sp., Hystrix sp., Poecillophora sp., Porites sp. dan Haliophora sp, dilakukan penapisan untuk melihat aktivitas kemampuannya melawan V. harveyi MR 5339 RfR dalam uji in vitro dan uji in vivo. Sebanyak 54 isolat teridentifikasi mampu menghasilkan zona hambat. Dua isolat (8A dan 1C) terbukti tidak bersifat patogen dan memiliki aktivitas probiotik melawan V. harveyi MR 5339 RfR pada uji in vitro dan in vivo. Tingkat kelangsungan hidup larva pada perlakuan yang diberi kandidat probiotik dari kedua isolat tersebut adalah 83,33%, dan menunjukkan hasil yang berbeda nyata dengan kontrol positif (61,67%). Laju pertumbuhan panjang larva yang diberi isolat 8A (5,25%) dan 1C (5,06%)menunjukkan hasil yang berbeda nyatajika dibandingkan dengan kontrolpositif (3,54%). Laju pertumbuhan bobot yang diberi isolat 8A (17,51%) dan 1C (17,61%) menunjukkan hasil yang berbeda nyata terhadap kontrol negative (15,27%) dan kontrol positif (14,69%).Kata kunci: probiotik, terumbu karang,udang windu, V. harveyi, vibriosis.

Selection of nitrification and denitrification bacteria with its application in culture medium of Pacific white shrimp (Litopenaeus vannamei) Widanarni, .; Pranoto, Sugiyo Hadi; Sukenda, .
Jurnal Akuakultur Indonesia Vol. 9 No. 2 (2010): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The aim of this study was to obtain nitrification and denitrification bacteria and tested its activity in reducing the ammonia, nitrite and nitrate compounds in culture medium of Pacific white shrimp Litopenaeusvannamei. The research was conducted through several steps: 1) Isolation of nitrification and denitrification bacteria from ponds and mangrove areas, 2) Selection of nitrification and denitrification bacteria in vitro, and 3) test of the nitrication and denitrification bacteria activities in culture medium of white shrimp. A total of 38 isolates of nitrification bacteria and 7 isolates of denitrification bacteria were successfully isolated from ponds and mangrove areas in the region of South Lampung and Subang, West Java. Of the total isolates were then selected in vitro based on its ability to reduce the compounds of ammonia, nitrite and nitrate and selected each 2 of the best isolates. Two select nitrification bacteria isolates, namely S11 and S12 each can oxidize ammonia at 78.25% and 80.54%, whereas two select denitrification bacteria isolates namely DS7 and DS3 each can reduce nitrate amounted to 85.41%, and 56, 49%. The test results of nitrification and denitrification bacteria activities in culture medium of white shrimp showed that S12-DS7 treatments gave the best result, with ammonia concentrations between 0.007 - 0.13 mg / l, nitrite of 0.04 -2.34 mg / l, nitrate of 1 0.33 - 3, 29 mg / l, shrimp growth rate of 2.1%, and survival rate of 90%.Keywords: nitrification, denitrification, Litopenaeus vannamei, water quality. ABSTRAKPenelitian ini bertujuan untuk mendapatkandan menguji aktivitasbakteri nitrifikasi dan denitrifikasi dalam mengurangi senyawa amonia, nitrit dan nitrat pada media budidaya udang vaname Litopenaeus vannamei. Penelitian dilakukan melalui beberapa tahap yaitu: 1) Isolasi bakteri nitrifikasi dan denitrifikasi dari lingkungan tambak dan mangrove, 2) Seleksi bakteri nitrifikasi dan denitrifikasi secara in vitro, dan 3) Uji aktivitas bakteri nitrifikasi dan denitrifikasi pada media budidaya udang vaname.Sebanyak 38 isolat bakteri nitrifikasi dan 7 isolat bakteri denitrifikasi berhasil diisolasi dari lingkungan tambak dan mangrove di daerah Lampung Selatan dan Subang, Jawa Barat. Dari total isolat tersebut kemudian diseleksi secara in vitro berdasarkan kemampuannya dalam menurunkan senyawa amonia, nitrit dan nitrat dan dipilih masing-masing 2 isolat terbaik. Dua isolat bakteri nitrifikasi terseleksi yaitu S11 dan S12 masing-masing dapat mengoksidasi amonia sebesar 78,25% dan 80,54%, sedangkan dua isolat bakteri denitrifikasi terseleksi yaitu DS3 dan DS7 masing-masing dapat mereduksi nitrat sebesar 85,41% dan 56,49%. Hasil uji aktivitas bakteri nitrifikasi dan denitrifikasipada media budidaya udang vaname menunjukkan bahwa perlakuan S12-DS7 memberikan hasil terbaik, dengan konsentrasi amonia berkisar antara 0,007-0,13 mg/l, nitrit 0,04-2,34 mg/l, nitrat 1,33-3,29mg/l,laju pertumbuhan udang sebesar 2,1%, dan kelangsungan hidup 90%.Kata kunci: nitrifikasi, denitrifikasi, Litopenaeus vannamei, kualitas air

Isolation and characterization of pathogenic Vibrio on tiger grouper Epinephelus fuscoguttatus Ilmiah, .; Sukenda, .; Widanarni, .; Harris, Enang
Jurnal Akuakultur Indonesia Vol. 11 No. 1 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This study was aimed to obtain pathogenic bacterial isolate causing vibriosis disease. Isolation of Vibrio was conducted from maribound tiger grouper collected from floating net cage in Barru Regency using TCBS medium. Ability to cause vibriosis was confirmed by pathogenicity test performed by mean injecting the tiger grouper juveniles with bacterial suspension at concentration of 106 CFU/fish and mortality of fish during seven days observation then was noted. Then, the Vibrio pathogenic isolate was characterized and identified based on morphology, growth, and biochemical features. Moreover, the most pathogenic isolate was identified by molecular analysis of 16S-rRNA gene sequences. The results showed that three potential isolates caused Vibriosis disease in tiger grouper culture. The isolates tested were biochemically identified as Vibrio metschnikovii, V. parahaemolyticus, and V. mimicus. The most virulent among isolates was V. parahaemolyticus. Keywords: isolation, characterization, pathogenic, vibriosis, tiger grouper

Selection of probiotic bacteria from the coral reef and tiger grouper fish (Epinephelus foscoguttatus) farming environment Ilmiah, .; Sukenda, .; Widanarni, .; Harris, Enang
Jurnal Akuakultur Indonesia Vol. 11 No. 2 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The use of probiotic bacteria is one of the methods in aquaculture to control infectious diseases. This study aims to obtain candidates probiotic bacteria from the coral reef and tiger grouper aquaculture environment (Epinephelus fuscoguttatus). Steps of the study included of: 1) isolation of candidates probiotic bacteria, 2) selection of candidates probiotic bacteria: inhibition test and co-culture methods, 3) enzymatic test, 4) the growth of bacteria test 5) pathogenicity test and 6) the molecular identification of probiotic bacteria. The results showed that of 124 isolates in test zone of inhibition, the four isolates with the widest zone of inhibition of isolate K7, K8, K21, and T4, a total of 51 isolates on co-culture methods, the four isolates were able to suppress the growth of pathogenic Vibrio bacteria, isolates K15, K68, K55, and T36. The results of hydrolyzing the highest protease amylase and lipase enzyme, respectively isolates K15 and T36; isolates T41 and K8; isolates K55 and K68. Candidates probiotic bacteria reached the exponential phase at 16‒18 h. The results of pathogenicity test isolate K7, K8, T36, and T41 provided the highest survival that can be used as candidates probiotics in the tiger grouper. Keywords: selection, probiotics, tiger grouper

Protein digestibility and ammonia excretion in catfish Clarias gariepinus culture Gunadi, Bambang; Harris, Enang; Supriyono, Eddy; Sukenda, .; Budiardi, Tatag
Jurnal Akuakultur Indonesia Vol. 12 No. 1 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT A series of experiments was performed to analyze protein digestibility, ammonia excretion, and also heterothropic bacteria and phytoplankton dynamics in the catfish Clarias gariepinus culture. In the digestibility experiment, catfish with an individual initial size of 43.67±0.83 g were stocked into 120 L conical fiberglass tanks at a density of 20 fish per tank. Fish were fed on with commercial diet supplemented with Cr2O3 indicator at a concentration of 1%. In the ammonia excretion experiment, catfish with an individual size of 111.6±9.5 and 40.6±3.4 g, respectively, were placed into a 10 L chamber filled with 8 L of water. Total ammonia nitrogen (TAN) in the chambers were monitored every hour for six consecutive hours. In the bacteria and phytoplankton dynamics experiment, catfish were stocked in the 25 m2 concrete tanks which was divided into two compartments (catfish 10 m2, and heterotrof compartments 15 m2). Catfish with individual size of 42,5±0 g were stocked into the tanks at a density of 100 fish per tank. Water was recirculated from catfish compartments to heterotrophic compartments. Fish were fed with floating feed. Molasses as carbon source for heterotrophic bacteria was applied daily. The experiment was conducted for six weeks. The results showed that the protein digestibility was 61.97±7.24%. Larger fish (size of 111.6 g) excreted ammonia at a rate of 0.008±0.003 mg TAN/g fish-weight/hour, which was lower than that of the smaller catfish (size of 40.6 g), i.e. 0.012±0.004 mg TAN/g fish-weight/hour. Keywords: protein digestibility, ammonia excretion, catfish ABSTRAK Serangkaian penelitian telah dilakukan untuk menganalisis ketercernaan pakan dan protein, ekskresi amonia, serta dinamika bakteri dan fitoplankton pada budidaya ikan lele (Clarias gariepinus). Pada penelitian ketercernaan pakan, ikan lele berukuran 43,67±0,83 g/ekor dipelihara dalam bak fiberglas berbentuk corong berukuran 120 L dengan kepadatan 20 ekor/bak. Ikan diberi pakan berupa pelet yang diberi indikator Cr2O3 sebanyak 1%. Pada penelitian ekskresi amonia, ikan lele berukuran 111,6±9,5 dan 40,6±3,4 g/ekor yang telah diberi makan sampai kenyang dimasukkan ke dalam stoples berisi 8 L air. Kadar amonia total (total ammonia nitrogen, TAN) di dalam stoples diukur setiap jam selama enam jam. Pada penelitian dinamika bakteri dan fitoplankton, ikan lele dipelihara pada bak beton berukuran 25 m2 yang disekat menjadi dua bagian yaitu bagian ikan lele (10 m2) dan bagian heterotrof (15 m2). Ikan lele dengan bobot awal 42,5 g/ekor ditebar ke dalam bak dengan kepadatan 100 ekor/bak. Air mengalir secara resirkulasi dari bagian ikan lele ke bagian heterotrofik dengan bantuan pompa. Pakan yang diberikan berupa pelet apung komersial. Molase ditambahkan setiap hari sebagai sumber karbon untuk pertumbuhan bakteri heterotrofik. Penelitian dilaksanakan selama enam minggu. Hasil pengamatan menunjukkan bahwa ketercernaan protein dari pakan yang diuji adalah 61,97±7,24%. Ikan lele berukuran besar (111,6 g/ekor) menghasilkan amonia sebanyak 0,008±0,003 mg TAN/g ikan/jam, lebih rendah dibandingkan dengan ikan yang berukuran lebih kecil (40,6 g/ekor), yaitu 0,012±0,004 mg TAN/g ikan/jam. Kata kunci: ketercernaan protein, ekskresi amonia, ikan lele

Kappa-carrageenan as immunostimulant to control infectious myonecrosis (IMN) disease in white shrimp Litopenaeus vannamei Febriani, Dian; Sukenda, .; Nuryati, Sri
Jurnal Akuakultur Indonesia Vol. 12 No. 1 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This study evaluated the modulation of non-specific immune response, growth, and disease resistance of white shrimp, Litopenaeus vannamei against infectious myonecrosis virus (IMNV). The first stage of this study evaluated the different dose of k-carrageenan administration i.e. 5, 10, and 15 g/kg feed for four weeks of rearing period, while the later studied evaluated about the frequency of administration i.e. daily, seven days interval, and 14-days interval for five weeks of rearing period. Both stages had positive and negative control and performed in complete randomized design. The parameters of observation consisted of immune parameters, clinical symptoms, growth, and survival. Shrimp were fed three times a day at feeding rate of 4–5% of body weight/day. IMNV Challenge test was performed by feeding the shrimp via oral route at a level 10% of body weight for three consecutive days, followed by 14-days observation. The results showed that shrimp administered with k-carrageenan at a concentration of 15 g/kg feed showed the best performance of all concentration tested. The shrimp’s haemocyte count, phagocytes activity, phenoloxidase activity, and relative growth were 12±0.72×106 cell/mL; 34.67±0.58%; 0.511±0.10; and 86.15% respectively. After challenged, the survival was 85±7.07%. Moreover, application in 14 days at 7-days interval gave 88.57% relative growth and 93±5,8% survival, which were higher than other treatments. The administration of k-carrageenan at concentration of 15 g/kg with 14 days interval on white shrimp juveniles showed higher immunostimulatory effect and better protection against IMNV. Keywords: kappa-carrageenan, immunostimulant, IMNV, Litopenaeus vannamei ABSTRAK Penelitian ini mempelajari pemberian kappa-karagenan dalam memodulasi respons imun nonspesifik, pertumbuhan, dan resistensi udang vaname Litopenaeus vannamei terhadap infeksi infectious myonecrosis virus (IMNV). Tahap pertama mengetahui dosis pemberian k-karagenan sebesar 5, 10, dan 15 g/kg pakan selama empat minggu pemeliharaan, sedangkan tahap kedua mengevaluasi frekuensi pemberian k-karagenan, yaitu setiap hari, tujuh hari, dan 14 hari secara berulang dengan interval tujuh hari selama lima minggu pemeliharaan. Kedua tahap penelitian menggunakan kontrol positif dan negatif dalam rancangan acak lengkap. Parameter pengamatan terdiri atas respons imun, gejala klinis, pertumbuhan dan kelangsungan hidup udang vaname. Udang diberi makan tiga kali sehari dengan FR 4–5% biomassa/hari. Infeksi IMNV dilakukan secara oral sebesar 10% biomassa selama tiga hari berturut-turut, dan diamati selama 14 hari. Hasil penelitian menunjukkan bahwa udang yang diberikan k-karagenan 15 g/kg pakan memperoleh hasil terbaik. Total hemosit, aktivitas fagositosis, aktivitas phenoloxidase, dan pertumbuhan relatif udang masing-masing adalah 12±0,72×106 sel/mL; 34,67±0,58%; 0,511±0,10 dan 86,15%, dengan kelangsungan hidup udang setelah diinfeksi IMNV sebesar 85±7,07%. Frekuensi pemberian 14 hari secara berulang dengan interval tujuh hari memberikan hasil kelangsungan hidup terbaik sebesar 88,57% dan pertumbuhan relatif sebesar 93±5,8%. Kesimpulan dari penelitian ini bahwa, pemberian k-karagenan 15 g/kg dan interval 14 hari menunjukkan respons imun dan perlindungan yang lebih baik terhadap IMNV. Kata kunci: kappa-karagenan, imunostimulan, IMNV, Litopenaeus vannamei

Screening of probiotics bacteria from coral reef using co-culture method for controlling vibriosis in tiger shrimp (Penaeus monodon) larvae Sasanti, Ade Dwi; ., Widanarni; ., Sukenda
Jurnal Akuakultur Indonesia Vol. 10 No. 2 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTThis study was carried out to obtain bacteria isolates from coral reef using co-culture method which potentially inhibit Vibrio harveyi growth. A total of 110 isolates were isolated from Acropora sp., Merulina sp., Hystrix sp., Poecillophora sp., Porites sp., and Haliophora sp., and were screened for their antagonistic activity against V. harveyi in in vitro and in vivo test. Five candidate probiotics (5H1 candidate probiotics isolated from Acropora sp., 11I and 11G isolates isolated from Hystrix sp. and 13B and 13G1 isolates isolated from Poecillophora sp., was able to inhibit growth of V. harveyi MR5339 RFR up to 101‒102 cfu/mL. Two isolates (13B and 13G1) were not pathogenic at concentration 106 cfu/mL bacteria and could increase of survival rate of tiger shrimp (Penaeus monodon) larvae in in vivo test. Survival rate of tiger shrimp larvae that treatment with 13B and 13G1 were 86,67% and 88,33%, and have a significant different with positive control (61,67%). Partial sequencing of 16S-rRNA showed that 13G1 isolate was similar to V. alginolyticus.Keywords: vibriosis, Vibrio harveyi, tiger shrimp, probiotic, coral reefABSTRAKPenelitian ini bertujuan untuk mendapatkan bakteri probiotik asal terumbu karang dengan metode kultur bersama untuk pengendalian penyakit vibriosis pada larva udang windu (Penaeus monodon). Sebanyak 110 isolat berhasil diisolasi dari Acropora sp., Merulina sp., Hystrix sp., Poecillophora sp., Porites sp., dan Heliophora sp. dan dilakukan penapisan untuk melihat aktivitas kemampuannya melawan Vibrio harveyi MR 5339 RfR dalam uji in vitro dan in vivo. Sebanyak 56 isolat menghasilkan daya hambat terhadap V. harveyi MR5339 RfR pada metode kultur bersama. Lima isolat kandidat probiotik (isolate 5H1 diisolasi dari Acropora sp., isolat 11I dan 11G diisolasi dari Hystrix sp., serta isolat 13B dan 13G1 yang diisolasi dari Poecillophora sp.), mampu menekan pertumbuhan V. harveyi MR5339 RfR hingga 101–102 cfu/mL. Kedua isolat (13B dan 13G1) terbukti tidak bersifat patogen pada konsentrasi 106 cfu/mL dan mampu meningkatkan sintasan larva udang windu pada uji in vivo. Nilai sintasan larva pada perlakuan yang diberi kandidat probiotik 13B dan 13G1 berturut-turut adalah 86,67% dan 88,33%, namun berbeda nyata dengan kontrol positif (61,67%). Hasil analisis sekuen sebagian gen 16S-rRNA menunjukkan bahwa isolat 13G1 termasuk spesies V. algynoliticus.Kata kunci: vibriosis, Vibrio harveyi, udang windu, probiotik, terumbu karang

Administration of Phyllanthus niruri to control IMNV (myonecrosis infectious virus) infection white shrimp Litopenaeus vannamei ., Sukenda; Nuryati, Sri; Sari, Isni Rahmatika
Jurnal Akuakultur Indonesia Vol. 10 No. 2 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTInfectious myonecrosis (IMN) disease is a major disease in Indonesia shrimp farming. The disease is caused by infectious myonecrosis virus (IMNV). Currently, treatment and drug has not been obtained to control the virus. This research was conducted to determine the effect of Phyllanthus niruri extract in white shrimp (Litopenaeus vannamei) against IMNV infection. Healthy shrimp was given P. niruri extract 20 mg/kg of feed for seven days and after that the shrimp was challenged by orally with IMNV infected shrimp tissue. The positive control was given feed without P. niruri extract and challenged with IMNV infected shrimp tissue, while negative control was not challenged with IMNV infected shrimp tissue. IMNV infection gave a significantly different effect on survival rate. In the shrimp P. niruri previously (86.7%) gave higher survival rate compared to shrimp without P. niruri (66.67%). Survival rate of negative control was 93.33%. IMNV clinical signs in general was white necrotic areas in striated muscles. Histological examination showed that cell necrosis appeared on the mussel tissues. In conclusion the addition of P. niruri to the commercial feed can give the survival rate of shrimp better when challenged with IMNV.Keywords: IMNV, Phyllanthus niruri, Litopenaeus vannameiABSTRAKPenyakit infectious myonecrosis (IMN) merupakan penyakit utama pada budidaya udang di Indonesia. Penyakit ini disebabkan oleh infectious myonecrosis virus (IMNV). Saat ini, belum diperoleh cara dan obat untuk mengendalikan virus IMNV. Penelitian ini dilakukan untuk mengevaluasi pengaruh immunostimulan tepung meniran (Phyllanthus niruri) yang diberikan melalui pakan pada udang vaname (Litopenaeus vannamei) yang diinfeksi IMNV. Udang vaname yang sehat diberi pakan yang mengandung meniran dengan dosis 20 mg/kg pakan selama tujuh hari dan kemudian diuji tantang secara oral dengan memberikan jaringan udang yang telah terinfeksi IMNV. Udang kontrol positif dilakukan dengan memberi pakan komersial tanpa penambahan meniran yang kemudian diuji tantang dengan memberi makan jaringan udang yang terinfeksi IMNV, sedangkan udang kontrol negatif tidak diuji tantang dengan jaringan yang terinfeksi IMNV. Hasil menunjukkan bahwa kelompok udang yang diberi pakan mengandung meniran mempunyai kelangsungan hidup (86,67%) lebih tinggi dibandingkan dengan udang yang tidak diberi pakan mengandung meniran (66,67%) ketika diuji tantang dengan IMNV. Kontrol negatif yang tidak diberi pakan mengandung meniran dan tidak diuji tantang dengan IMNV memberikan kelangsungan hidup 93,33%. Gejala klinis yang ditunjukkan adanya infeksi IMNV terlihat dengan adanya otot putih pada ruas tubuh udang. Pengamatan histopatologi menunjukkan adanya nekrosis pada sel-sel di jaringan otot udang. Sebagai kesimpulan dapat dinyatakan bahwa penambahan meniran pada pakan komersial dapat meningkatkan kelangsungan hidup udang ketika terjadi infeksi IMNV.Kata kunci: IMNV, Phyllanthus niruri, Litopenaeus vannamei

Application of synbiotic with different probiotic doses to prevent vibriosis in humpback grouper Saputra, Dwi Agung; Sukenda, ,; Widanarni, ,
Jurnal Akuakultur Indonesia Vol. 12 No. 2 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The aim of this study was to evaluate the supplementation of synbiotic with different doses of probiotics to prevent vibriosis in humpback grouper (Cromileptes altivelis). Grouper (3.0±0.48 g) fed by control feeds (without supplementation of synbiotic) which were K(-) and K(+), treatment feeds with supplementation of synbiotic with different doses of probiotic (probiotic 104 cfu/mL) 1% + prebiotic 2% (P1) v/w, (probiotic 106 cfu/mL) 1% + prebiotic 2% (P2) v/w, (probiotic 108 cfu/mL)1% + prebiotic 2% (P3) v/w) for 30 days. After a feeding trial period, there were observation of the bacterial counts in the fish intestine, the fish growth performance and immune response. Then all the grouper were challenged by Vibrio alginolyticus, except K (-). This study showed that survival, daily growth and food conversion ratio (FCR) of grouper in treatment P2 ((probiotic 106 cfu/mL) 1% + prebiotic 2%) and P3 (probiotic (108 cfu/mL) 1% + prebiotic 2%) were significantly better (P>0.05) than controls. The different doses of probiotic in synbiotic (probiotic 104 cfu/mL, probiotic 106 cfu/mL and probiotic 108 cfu/mL) provided better immune response than controls. Keywords: synbiotic, Vibrio alginolyticus, Bacillus sp., Cromileptes altivelis ABSTRAK Tujuan penelitian ini adalah mengkaji pemberian sinbiotik dengan dosis probiotik berbeda untuk mencegah penyakit vibriosis pada ikan kerapu bebek (Cromileptes altivelis). Ikan kerapu dengan berat (3,0±0,48 g) diberikan pakan kontrol (tanpa penambahan sinbiotik) K(-) dan K(+), pakan perlakuan dengan penambahan sinbiotik dengan dosis probiotik berbeda: probiotik ((104 cfu/mL) 1% + prebiotik 2%) v/w (P1), ((probiotik 106 cfu/mL) 1% + prebiotik 2%) v/w (P2), dan ((probiotik 108 cfu/mL)1% + prebiotik 2%) v/w (P3) selama 30 hari. Setelah perlakuan pakan sinbiotik, dilakukan pengamatan terhadap jumlah total bakteri di usus, kinerja pertumbuhan dan respons imun. Kemudian ikan kerapu pada seluruh perlakuan, kecuali kontrol negatif (-) diberi uji tantang dengan Vibrio alginolyticus. Hasil penelitian menunjukkan bahwa sintasan, laju pertumbuhan harian (LPH), dan rasio konversi pakan (FCR) pada perlakuan P2 ((probiotik 106 cfu/mL) 1% + prebiotik 2%) dan P3 (probiotik (108 cfu/mL) 1% + prebiotik 2%) secara signifikan lebih baik (P<0,05) bila dibandingkan dengan kontrol. Perlakuan sinbiotik dengan dosis probiotik berbeda (probiotik 104 cfu/mL, probiotik 106 cfu/mL, dan probiotik 108 cfu/mL) juga memberikan respons imun yang lebih baik dibandingkan dengan kontrol. Kata kunci : sinbiotik, Vibrio alginolyticus, Bacillus sp., Cromileptes altivelis

Co-Authors , Rahman, , , Ranta, , , Rusli, , . ARIFUDDIN . Maryani . Rahman . Sunarto A. Hasan A. Santika A. Suwanto A.J. Sihombing Ade Dwi Sasanti Aditya, Tiya Widi Afif, Usamah Afiff , Usamah Aldy Mulyadin Alimuddin Alit Brilliant Aminatul Zahra ANGELA MARIANA LUSIASTUTI Angela Mariana Lusiastuti Anis Nugrahawati Annisa Astri Anggraeni Ayi Santika Badrudin, Deni Ramdani Bambang Gunadi Benny Yustim Brite, Margie Catur A. Pebrianto D. Dana D. Meha D. Wahjuningrum Daniel Djokosetiyanto DEBY YUNIASARI Dendi Hidayatullah, Dendi Dewi Rahmi DIAH AYU SATYARI UTAMI Dian Febriani Dinamella Wahjuningrum Dwi Agung Saputra E. Ayuzar E. Harris Eddy Supriyono Eka Angga Laksana Enang Harris Enang Harris Esti Handayani Hardi Evan Farhan Wahyu Puadi F.H. Pasaribu Fachriyan Hasmi Pasaribu Fadlilah, Rizqy Aditya Fitria Novianti Gustilatov, Muhamad Henky Manoppo Hisatsugu Wakabayashi I. Normalina I. Nur Iqbal Kurniawinata, Mohamad Irzal Effendi Isni Rahmatika Sari Jasmanindar, Yudiana Jeanni Indah Noermala Julie Ekasari K. Sumantadinata Khairun Nissa Komar Sumantadinata Kukuh Nirmala L. Jamal Lastriliah, Mira M. Yuhana M. Zairin Junior M.S. Arifin Mahendra, Randa Mia Setiawati Mochamad Fatuchri Sukadi Muchtar, Muthahharah Muh. Aris Muh. Fatuhcri Sukadi Muharram Nur Ikhsan Mulyani, Rahma MUNTI YUHANA Muttaqin, Helmy Faisal N.A. Maswan Nasri Julaini Nasrullah, Hasan Nur Bambang Priyo Utomo Nuzullia, Laely Odang Carman Ode, Inem P. Hadi Pras, Eva Prasetiyono Pratiwi, Kiki Amalia R.D. Soejoedono Rafsyanzani, Muhammad Mufthi Rahman Rahman Ramadhani, Dian Eka Ramhirez, Putri Shandra Retno Damayanti Soejoedono Ririn Nurul Fauziah, Ririn Nurul Rizki Praseto, Rizki Rr. Bellya Anasti Maharani Rudi, Mad Ruku Ratu Borut Samsu Adi Rahman Sari Anggraeni, Sukma Septiani, Ghita Ryan Sri Nuryati Sri Nuryati Sugeng Budiharsono Sugiyo Hadi Pranoto Tatag Budiardi Trian Rizky Febriansyah Tsani Untsa, Agista Uba Umbara Ulil Surtia Zulpratita Umbara, Uba Wesly Pasaribu WIDANARNI WIDANARNI Wisriati Lasima Y. Tri Anggoro Yan Evan Yumaidawati, Nurfitriani Siti Yuni Puji Hastuti Zulfani, Anisa

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