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Sukenda .

Departemen Budidaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan, Institut Pertanian Bogor Kampus IPB Darmaga Bogor 16680

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Whole-cell vaccine of Streptococcus agalactiae in Oreochromis sp. with immersion method Sukenda, ,; Febriansyah, Trian Rizky; Nuryati, Sri
Jurnal Akuakultur Indonesia Vol. 13 No. 1 (2014): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The study was aimed to evaluate the efficacy of formalin-killed non-hemolytic Streptococcus agalactiae N14G and NK1 isolates whole-killed vaccine to prevent streptococcosis in tilapia. Ten fishes were reared in a tank 60x30x35 cm3 with an average body weight at 10.79±0.99 g. Fish was vaccinated through bath immersion at a concentration of 109 cfu/mL. Fish was subsequently challenged by intraperitonial injection of Streptococcus agalactiae 105 cfu/mL at 11 days post-vaccination. Parameters observed were survival, relative percent survival (RPS), total leukocyte, phagocytic activity, antibody titer, total erythrocyte, haemoglobin level, haematocrit level, dan water quality. Samplings were performed in day-0, 20, and 30 after vaccination. Both vaccines have shown higher survival (60%) and RPS (40%) when challenged with pathogenic Streptococcus N14G isolates than other treatments. Based on RPS percentage observed, those vaccine were still not sufficiently effective to combat S. agalactiae infection. Keywords: tilapia, bath immersion, Streptococcus agalactiae, whole-cell vaccine ABSTRAK Penelitian ini bertujuan untuk mengevaluasi efikasi vaksin formalin-killed cell Streptococcus agalactiae tipe isolat nonhemolitik N14G dan NK1 se utuh yang diberikan melalui perendaman dalam mencegah penyakit streptococcosis pada ikan nila. Ikan nila yang digunakan memiliki bobot 10,79±0,99 g, dipelihara sebanyak sepuluh ekor dalam akuarium ukuran 60x30x35 cm3. Ikan divaksinasi dengan metode perendaman dengan dosis 109 cfu/mL. Uji tantang dilakukan pada hari ke-11 pascavaksinasi dengan dosis 105 cfu/mL. Parameter yang diamati meliputi sintasan (SR), sintasan relatif/relative percent survival (RPS), total leukosit, aktivitas fagositik, titer antibodi, total eritrosit, kadar hemoglobin, kadar hematokrit, dan kualitas air. Pengamatan parameter dilakukan pada hari ke-0, ke-10, ke-20, dan ke-30. Hasil penelitian menunjukkan perlakuan kedua vaksin yang diinfeksi bakteri patogen isolat N14G memberikan nilai sintasan dan nilai RPS tertinggi dibanding perlakuan lainnya. Nilai sintasan dan RPS kedua perlakuan tersebut adalah 60% dan 40%. Nilai RPS yang cukup kecil menunjukkan vaksin yang diberikan masih kurang efektif untuk mencegah infeksi bakteri S. agalactiae. Kata kunci: ikan nila, perendaman, Streptococcus agalactiae, vaksin sel utuh

Effectiveness of sinbiotic at different doses in Pacific white shrimp pond culture Sukenda, ,; Praseto, Rizki; Widanarni, ,
Jurnal Akuakultur Indonesia Vol. 14 No. 1 (2015): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The increasing demand of white shrimp Litopenaeus vannamei requires the application of intensive culture system. However, intensive culture system of white shrimp could increase the risk of disease outbreak. The application of sinbiotic may provide solution to the problem. This study was aimed to evaluate the effectiveness of the use of technical sinbiotic on the survival and growth of white shrimp in ponds. This study consisted of four treatments; treatment K (control), treatment A (probiotic 0.5% and prebiotic 1%), treatment B (probiotic 1% and prebiotic 2%), and treatment C (probiotic 2% and prebiotic 4%). The results showed that administration of sinbiotic had no significant differences on survival rate, growth rate, feed conversion ratio, size, and biomass of shrimp (P>0.05). However, based on analysis of business, sinbiotic A provided higher profits to the farmer (Rp10.230) compared to other symbiotic treatments and control. Keywords: Litopenaeus vannamei, sinbiotic, technical media ABSTRAK Permintaan terhadap udang vaname Litopenaeus vannamei yang semakin meningkat membuat sistem budidaya udang sebaiknya menggunakan sistem budidaya intensif. Namun demikian sistem intensif pada budidaya udang vaname dapat meningkatkan risiko timbulnya penyakit. Penggunaan sinbiotik diharapkan dapat memberikan solusi dalam mengatasi masalah tersebut. Penelitian ini bertujuan untuk menguji efektivitas penggunaan sinbiotik teknis terhadap sintasan, pertumbuhan, dan keuntungan usaha udang vaname yang dipelihara di tambak. Penelitian ini terdiri atas empat perlakuan, yaitu perlakuan K (kontrol), perlakuan A (probiotik 0,5% dan prebiotik 1%), perlakuan B (probiotik 1% dan prebiotik 2%), dan perlakuan C (probiotik 2% dan prebiotik 4%). Hasil penelitian menunjukkan bahwa pemberian sinbiotik tidak berbeda nyata dalam nilai sintasan, laju pertumbuhan, rasio konversi pakan, size, dan biomassa udang (P>0,05). Namun demikian, berdasarkan analisis usaha perlakuan A memberikan keuntungan lebih tinggi (Rp10.230) dibandingkan dengan perlakuan sinbiotik lainnya serta kontrol. Kata kunci: Litopenaeus vannamei, sinbiotik, media teknis

Combination of garlic - shatterstone herb powder to control Streptococcus agalactiae infection in tilapia Fauziah, Ririn Nurul; Wahjuningrum, Dinamella; Sukenda, ,; Ranta, ,
Jurnal Akuakultur Indonesia Vol. 14 No. 1 (2015): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This study was aimed at determining potential of combination powder of garlic Allium sativum-shatterstone herb Phyllanthus niruri supplemented in feed against S. agalactiae infection in tilapia. Four concentrations of combination powder of A. sativum-P. Niruri; 20+5, 20+10, 20+15 and 20+20 ppt respectively were investigated for their ability to inhibit bacterial fish pathogen. Combination dose of 20+15 ppt produced the highest inhibitory zones in in vitro test. In vivo test consisted of three treatments with three replications, namely positive control (K+), negative control (K-) and the treatment of A. sativum-P. niruri suplemented in feed (BM). The test perfomed on tilapia with weight of 10.33 ± 1.63 g and were reared at density of 10 ind/aquarium. The fish was fed for 14 days, then injected intraperitoneally with 0.1 mL S. agalactiae at concentration of 105 cfu/mL for positive control and BM groups. Survival, growth rate, feed response, hematological and water quality parameters were observed for 10 days. This study showed that the suplemented-feed-fish (BM) showed better growth rate, feed response, and survival (83.3%) than positive control (36.7%) at P<0.05. In addition, A. sativum-P. niruri suplemented in feed was also able to enhance the immune response by increasing phagocytic activity. Keywords: Streptococcus agalactiae, phytopharmacy, Allium sativum-Phyllanthus niruri, tilapia ABSTRAK Penelitian ini bertujuan untuk menganalisis potensi campuran tepung bawang putih Allium sativum-meniran Phyllanthus niruri dalam pakan terhadap pencegahan infeksi bakteri S. agalactiae pada ikan nila. Empat konsentrasi campuran tepung bawang putih-meniran yaitu 20+5 ppt, 20+10 ppt, 20+15 ppt dan 20+20 ppt masing-masing diuji kemampuannya dalam menghambat bakteri patogen pada ikan. Campuran dosis 20+15 ppt menghasilkan zona hambat terbaik dalam uji in vitro. Uji in vivo terdiri atas tiga perlakuan dengan tiga ulangan yaitu kontrol positif, kontrol negatif, dan perlakuan pakan yang mengandung bawang putih-meniran (BM). Uji ini dilakukan pada ikan nila berbobot 10,33±1,63 g yang dipelihara di akuarium dengan kepadatan 10 ekor/akuarium. Ikan diberi pakan perlakuan selama 14 hari kemudian diinjeksi secara intraperitoneal dengan bakteri S. agalactiae sebanyak 0,1 mL dengan kepadatan 105 cfu/mL pada perlakuan kontrol positif dan perlakuan BM. Parameter kelangsungan hidup, laju pertumbuhan, respons pakan, parameter hematologi, dan kualitas air diamati selama sepuluh hari. Hasil dari penelitian ini menunjukkan bahwa pemberian BM dalam pakan memberikan laju pertumbuhan, respons pakan, dan sintasan (83,3%) yang lebih baik daripada kontrol positif (36,7%) pada P<0,05. Pakan yang mengandung campuran bawang putih-meniran ini juga mampu meningkatkan respons imun dengan adanya peningkatan aktivitas fagositosis. Kata kunci: Streptococcus agalactiae, fitofarmaka, Allium sativum-Phyllanthus niruri, ikan nila

The protective duration of Streptococcus agalactiae vaccine in Nile Tilapia for the prevention of streptococcosis Sukenda, ,; Rusli, ,; Nuryati, Sri; Hidayatullah, Dendi
Jurnal Akuakultur Indonesia Vol. 14 No. 2 (2015): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The aim of this study was to assess the protective duration of Streptococcus agalactiae vaccine against streptococcosis in Nile tilapia. Fish were treated by the whole cell vaccine, ECP vaccine and mixture of whole cell and ECP vaccine. After 14, 28, 42, and 52 day post-vaccination (DPV), the fish were intraperitoneally challenged with 104 cfu/mL S. agalactiae. The results showed mortality rate of whole-cell vaccine (A), ECP vaccine (B) and mix vaccine (C) up to day 42 was significantly (P<0.05) lower than the control treatment, namely 73.33%; 80%; and 76%, respectively. The mortality rate of vaccine treatments A, B, and C on day 56 had no significant difference (P>0.05) with the control. The value of antibody titer vaccine treatments A, B, and C indicate that antigen-antibody reaction on day 28 after the vaccination was significantly (P <0.05) higher than the control that were 3.67; 3.33; and 3.67. Antigen-antibody reaction on day 42 after the vaccination was founded, but did not different significantly (P>0.05) with the control. Bacterial population in treatment A, B, and C in the organs of the fish until the 28th day was still under the control of 104 cfu/mL. S. agalactiae vaccine protection duration is 42 days after the vaccination. Keywords : nile tilapia, Streptococcus agalactiae, duration, vaccine, streptococcosis ABSTRAK Penelitian ini bertujuan untuk menganalisis durasi proteksi dari vaksin Streptococcus agalactiae sebagai pencegahan terhadap streptococcosis pada ikan nila. Ikan divaksinasi dengan vaksin sel utuh, ECP dan gabungan sel utuh dan ECP dari S. agalactiae yang diinjeksi secara intrapetorineal. Ikan diuji tantang S. agalactiae 104 cfu/mL pada hari ke-14, ke-28, ke-42, dan ke-56 pascavaksinasi. Hasil penelitian menunjukkan tingkat mortalitas perlakuan vaksin sel utuh (A), vaksin ECP (B), dan gabungan vaksin sel utuh dengan ECP (C) hingga hari ke-42 masih signifikan (P<0,05) lebih rendah dari perlakuan kontrol yaitu 73,33%; 80%; dan 76%. Tingkat mortalitas perlakuan vaksin A, B, dan C pada hari ke-56 sudah tidak berbeda signifikan (P>0,05) dengan kontrol. Nilai titer antibodi perlakuan vaksin A, B, dan C menunjukkan bahwa reaksi antigen antibodi pada hari ke-28 pascavaksinasi masih signifikan (P<0,05) lebih tinggi dibandingkan kontrol yaitu 3,67; 3,33; dan 3,67. Reaksi antigen antibodi pada hari ke-42 pascavaksinasi masih ditemukan, namun tidak berbeda signifikan (P>0,05) dengan kontrol. Populasi bakteri pada perlakuan A, B, dan C di organ ikan hingga hari ke-28 masih di bawah kontrol 104 cfu/mL. Durasi proteksi vaksin S. agalactiae adalah 42 hari pascavaksinasi. Kata kunci: ikan nila, Streptococcus agalactiae, durasi, vaksin, streptococcosis

Infektivitas parasit Ichtyophthirius multifiliis yang disimpan pada suhu rendah Rahman, ,; Sukenda, ,; Nuryati, Sri; Hidayatullah, Dendi
Jurnal Akuakultur Indonesia Vol. 15 No. 2 (2016): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The aim of this study was to evaluate infectivity of Ichtyophthirius multifiliis which caused white spot disease maintained at low temperature without its host. Briefly, the trophont stage of parasites were subjected at control (28 °C) and lower temperature (9 °C) for 14 consecutive days of observation. The rate of survival, and excystment of descendants were examined descriptively at the last day of observation. Here, the infectivity of parasite then performed by means infecting the model fish Poecilia sphenops (black moly) with escaping theronts. The results revealed that the survival rate and excystment rate of parasite were decreased as maintaining period increased. The final rate of survival, and excystment of parasite were 35% and 33,3% respectively. Additionally, the descendants came out with high abnormality which recognized by weak mobility and lower infectivity (50%) compared to the control (80%). Then, it is concluded that, maintaining I. multifiliis at low temperature without its host for 14 consecutive days will decreased the infectivity. Keywords: white spot, obligat parasite, excystment, infectivity ABSTRAK Penelitian ini bertujuan untuk mengevaluasi infektivitas parasit Ichtyophthirius multifiliis penyebab penyakit bintik putih (white spot) yang dipelihara tanpa inang pada suhu rendah. Parasit dengan stadia trophont dipelihara pada suhu ruang (28 °C) dan suhu rendah (9 °C) selama 14 hari. Selama masa pemeliharaan tersebut tingkat kelulusan hidup, dan tingkat eksismen parasit diukur dan dibandingkan secara deskriptif. Hari terakhir pemeliharaan dilakukan uji tantang pada ikan black moly Poecilia sphenops untuk menilai infektivitas parasit. Hasil penelitian memperlihatkan bahwa tingkat kelulusan hidup dan eksismen parasit semakin menurun dengan bertambahnya masa pemeliharaan. Akhir pengamatan kelangsungan hidup, dan nilai eksismen tersebut berturut-turut adalah 35% dan 33%. Parasit yang disimpan pada suhu rendah selama 14 hari memperlihatkan infektivitas yang lebih rendah (50%) dibandingkan dengan perlakuan kontrol (80%). Kesimpulannya, penyimpanan parasit I. multifiliis pada suhu rendah selama 14 hari dapat menurunkan infektivitas parasit pada inang. Kata kunci: bintik putih, parasit obligat, eksismen, infektivitas

Kinerja probiotik Bacillus sp. pada pendederan benih ikan lele Clarias sp. yang diinfeksi Aeromonas hydrophila Sukenda, ,; Rafsyanzani, Muhammad Mufthi; Rahman, ,; Hidayatullah, Dendi
Jurnal Akuakultur Indonesia Vol. 15 No. 2 (2016): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This experiment was conducted to assess performance of Bacillus sp. probiotic on catfish juvenile Clarias sp. infected by Aeromonas hydrophila. The probiotic content in the diets were 0% (K+ and K-), 1%, and 2% in duplicates. This experiment used randomized design with four treatments and two replications. Juveniles with average body weight of 3.22±0.15 g/fish were reared in the 1.5×2.8×0.5 m3 pond with density of 800 fish/pond. Fish were reared for 30 days and fed three times a day at rate 8% of total body weight. At day 31, catfish were challenged by A. hydrophila 0.1 mL (106 cfu/mL). Post infection observation was carried out ten days with density 10 fish/aquaria. The result showed that fish fed diet containing 2% probiotic gave the best probiotic performance with survival rate of catfish 83.33% after challenged, spesific growth rate 5.40%, and 0,75 of feed conversion ratio. The results of the blood profile showed significantly better results in the treatment of probiotics compared to the positive control after challenge test A. hydrophila. Probiotic Bacillus sp. has given as much as 2% on feed provides better performance on catfish juvenile. Keywords: probiotic, Bacillus sp., A. hydrophila, catfish juvenille, growth ABSTRAK Penelitian ini bertujuan untuk menguji kinerja probiotik Bacillus sp. dalam pakan pada pendederan benih ikan lele Clarias sp. yang diinfeksi bakteri Aeromonas hydrophila. Penelitian ini menggunakan rancangan acak lengkap dengan empat perlakuan yaitu kandungan probiotik dalam pakan perlakuan yaitu 0% (K+ dan K-), 1%, dan 2%, masing-masing dengan dua ulangan. Ikan lele yang digunakan memiliki bobot rata-rata 3,22±0,15 g/ekor, dipelihara dalam kolam terpal berukuran 1,5×2,8×0,5 m3 dengan kepadatan 800 ekor/kolam. Ikan dipelihara selama 30 hari dengan frekuensi pemberian pakan tiga kali sehari sebanyak 8% dari bobot tubuh ikan. Hari ke-31 benih lele diinjeksi bakteri A. hydrophila dosis 0,1 mL/ekor dengan kepadatan bakteri 106 cfu/mL. Pemeliharaan setelah diinfeksi dilakukan selama sepuluh hari dengan kepadatan 10 ekor/akuarium. Hasil penelitian menunjukkan ikan yang diberi probiotik 2% memperlihatkan kinerja probiotik terbaik dengan tingkat kelangsungan hidup ikan lele sebesar 83,33% setelah diinfeksi dengan A. hydrophila; laju pertumbuhan harian sebesar 5,40%; dan konversi pakan 0,75. Hasil gambaran darah menunjukkan hasil yang signifikan lebih baik pada perlakuan pemberian probiotik dibandingkan kontrol positif pascauji tantang A. hydrophila. Probiotik Bacillus sp. yang diberikan sebanyak 2% pada pakan memberikan kinerja lebih baik pada pendederan benih ikan lele. Kata kunci: probiotik, Bacillus sp., A. hydrophila, benih lele, pertumbuhan

Efficacy of whole cell vaccine Aeromonas hydrophila on catfish broodstock and it’s offspring resistance againt motile aeromonad septicemia (MAS) Sukenda, ,; Pratiwi, Kiki Amalia; Rahman, ,; Hidayatullah, Dendi
Jurnal Akuakultur Indonesia Vol. 16 No. 1 (2017): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Transfer of maternal immunity by mean of passive immunization is a way to provide protection and durability of antibodies on the offspring. The purpose of this research was to analize effication of Aeromonas hydrophila vaccine on the catfish broodstock to maternal transfer of immunity, and offspring resistance. The average body weight of broodstock used in this study were 650±50 g were kept in pool tarps sized 2×1×0.5 m3. This study used a randomized complete design with two treatments and three replications. Female broodstock were vaccinated using intraperitonial injections at a dose 0.4 mL/kg and control fish were injected with phospate buffered saline (PBS). The observed parameters include hematology of broodstock, mortality, the relative survival rate, and antibody titers. Antibody titer measurements on broodstock, eggs, and offspring. Vaccination on broodstock catfish delivers a significant antibody level (P<0.05) on offspring compared to control catfish with relative survival rate of offspring at 5, 10, and 15 days after hatching were 67.76%, 82.66%, and 71.66% respectively. Keywords: catfish, Aeromonas hydrophila, vaccination, antibody transfer ABSTRAK Transfer kekebalan dari induk kepada benih melalui imunisasi pasif merupakan salah satu cara untuk memberikan proteksi pada benih. Tujuan penelitian ini untuk menguji efikasi vaksin sel utuh Aeromonas hydrophila pada induk ikan nila dalam mentransfer kekebalan spesifik ke benih dan menguji ketahanan benih hasil pemijahan induk yang divaksin. Induk lele yang digunakan pada penelitian ini memiliki bobot rata-rata 650±50 g dipelihara di kolam terpal berukuran 2×1×0,5 m3. Penelitian ini menggunakan rancangan acak lengkap dengan dua perlakuan dan tiga ulangan. Induk betina lele divaksinasi secara intraperitonial dengan dosis 0,4 mL/kg ikan dan induk lele kontrol disuntik dengan phospate buffer saline (PBS). Parameter yang diamati meliputi hematologi induk, mortalitas, tingkat kelangsungan hidup relatif benih, dan titer antibodi. Vaksinasi induk lele memberikan hasil level antibodi yang signifikan (P<0,05) pada induk, telur, dan benih lele dibandingkan perlakuan kontrol dengan tingkat kelangsungan hidup relatif benih umur 5, 10, dan 15 hari pacatetas masing-masing sebesar 67,76; 82,66%; dan 71,66%. Kata kunci: ikan lele, Aeromonas hydrophila, vaksinasi, transfer antibodi

Extract of seaweed Gracilaria verrucosa as immunostimulant to controlling white spot disease in Pacific white shrimp Litopenaeus vannamei Zahra, Aminatul; Sukenda, Sukenda; Wahjuningrum, Dinamella
Jurnal Akuakultur Indonesia Vol. 16 No. 2 (2017): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This experiment was conducted to examine effect of Gracilaria verrucosa extract in diet with different dosages to enhance immune response and resistance against white spot syndrome virus (WSSV) in the Pacific white shrimp. The experiment consisted of six treatments in three replicates respectively, namely K- (without extract), K + (without extract + infected WSSV), A (2 g/kg of feed + infected WSSV), B (3 g/kg of feed + infected WSSV), C (4 g/kg of feed + infected WSSV), and D (5 g/kg of feed + infected WSSV). White shrimp with initial body weight of 6.07±0.10 g were reared in the (60×30×30) cm with density of 10 shrimps/aquarium. G. verrucosa was extracted with ethyl acetate. Pacific white shrimp had been fed medicated feed three times daily 3% at satiation for 14 days. At 15th days, white shrimp were challenged with WSSV at 0.1 mL/shrimp intramuscularly. The results showed that the immune response shrimp (total hemocyte count, phagocytic activity, respiratory burst, and phenoloxidase activity) fed medicated feed increased significantly compared to positive and negative controls. The best relative percent survival post-challenge test was at 4 g/kg dose of G. verrucosa, i.e 41.07±3.09%. Confirmation of WSSV using PCR showed that shrimps (A, B, C, D, and K+) were positively infected by WSSV. It was concluded that 4 g/kg dose of G. verrucosa gave the best result to enhance immune response and resistance to WSSV infection. Keywords: seaweed, Gracilaria verrucosa, immunostimulant, Pacific white shrimp, WSSV ABSTRAK Penelitian ini bertujuan untuk menguji pengaruh pemberian ekstrak Gracilaria verrucosa melalui pakan dengan dosis yang berbeda untuk meningkatkan imunitas dan resistensi udang vaname terhadap serangan white spot syndrome virus (WSSV). Penelitian ini terdiri atas enam perlakuan dan masing-masing tiga ulangan, yaitu K- (tanpa ekstrak), K+ (tanpa ekstrak + infeksi WSSV), A (2 g/kg pakan + infeksi WSSV), B (3 g/kg pakan + infeksi WSSV), C (4 g/kg pakan + infeksi WSSV), dan D (5 g/kg pakan + infeksi WSSV). Udang vaname dengan bobot 6,07±0,10 g dipelihara dalam akuarium dengan ukuran (60×30×30) cm dengan padat tebar 10 ekor/akuarium. Udang diberi pakan perlakuan secara at satiation sebanyak tiga kali sehari selama 14 hari. Pada hari ke-15 diuji tantang dengan WSSV pada dosis 0,1 mL/ekor secara intramuskular. Hasil penelitian menunjukkan bahwa respons imun udang (total hemosit, aktivitas fagositik, respiratory burst, dan aktivitas fenoloksidase) yang diberi pakan mengandung ekstrak G. verrucosa mengalami peningkatan signifikan dibanding perlakuan kontrol positif maupun negatif. Relative percent survival terbaik pasca uji tantang pada perlakuan C (4 g/kg), yaitu 41,07±3,09%. Konfirmasi WSSV dengan menggunakan PCR menunjukkan hasil bahwa udang (A, B, C, D, and K+) positif terinfeksi WSSV. Disimpulkan bahwa dosis ekstrak G. verrucosa 4 g/kg pakan memberikan hasil terbaik untuk meningkatkan respons imun pada udang vaname dan resistensi terhadap WSSV. Kata kunci: rumput laut, Gracilaria verrucosa, imunostimulan, udang vaname, WSSV

Vaccination in Nile tilapia broodstock with whole cell vaccine and disease resistance in its fry against Aeromonas hydrophila Sukenda, Sukenda; Carman, Odang; Rahman, Rahman; Hidayatullah, Dendi; Yumaidawati, Nurfitriani Siti
Jurnal Akuakultur Indonesia Vol. 16 No. 2 (2017): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The aim of this study was to analyze the effectivity of vaccination in Nile tilapia broodstock with whole cell vaccine and disease resistance in fry tilapia against Aeromonas hydrophila. Tilapia Nirwana strain that used for this had average body weight of 185±13.23 g and were maintained in ponds sizing of (2.5×2.5×1 m3). Vaccinations that has been done through intraperitoneal injection using dose of 0.1 mL/fish, meanwhile the fish for control was injected by phosphate buffered saline (PBS). This study used complete randomized design with two treatments and three replications. Antibody level was measured by using indirect enzyme-linked immunosorbent assay (ELISA) method in the broodstock, egg, and fry. Challenge test in fry tilapia performed at the age of 5, 10, and 15 days. The results showed that vaccination in tilapia broodstock delivered a significant antibody level in broodstock, eggs, and fry (P<0.05) compared to the control. Relative percent survival of offspring at 5, 10, and 15 days were 78.26%, 70.59%, and 65.52%, respectively. As a conclusion, vaccination in tilapia broodstock was effective to improve specific and non-specific immunity, and protect fry tilapia from A. hydrophila infection through maternal immunity. Keywords: vaccination, antibody, maternal immunity, tilapia, Aeromonas hydrophila ABSTRAK Penelitian ini bertujuan untuk menganalisis efikasi vaksinasi pada induk nila dengan vaksin sel utuh dan ketahanan benih yang dihasilkan terhadap Aeromonas hydrophila. Ikan nila stain Nirwana yang digunakan dalam penelitian memiliki bobot rata-rata 185±13,23 g dan ikan dipelihara dalam kolam (2,5×2,5×1 m3). vaksinasi dilakukan melalui penyuntikan intraperitoneal dengan dosis 0,1 mL/ikan, sementara itu ikan kontrol disuntik dengan phosphate buffered saline (PBS). Penelitian ini menggunakan rancangan acak lengkap dengan dua perlakuan dan tiga ulangan. Tingkat antibodi diukur dengan menggunakan metode indirect enzyme-linked immunosorbent assay (ELISA) pada induk, telur dan benih. Uji tantang pada benih dilakukan pada umur 5, 10, dan 15 hari. Hasil penelitian menunjukan bahwa vaksinasi pada induk nila secara signifikan dapat meningkatkan level antibodi pada induk, telur, dan benih (P<0,05) dibandingkan dengan kontrol. Kelangsungan hidup relatif pada benih berumur 5, 10, dan 15 hari masing-masing adalah 78,26%; 70,59%; dan 65,52%. Sebagai kesimpulan vaksinasi pada induk nila efektif dalam memperbaiki imunitas spesifik dan non spesifik serta melindungi benih dari infeksi A. hydrophila melalui imunitas maternal. Kata kunci: vaksinasi, antibodi, imunitas maternal, ikan nila, Aeromonas hydrophila

The use of immunostimulant from phycocyanin of Spirulina platensis to control motile aeromonad septicaemia (MAS) disease in common carp Cyprinus carpio Muchtar, Muthahharah; Sukenda, Sukenda; Nuryati, Sri; Hidayatullah, Dendi
Jurnal Akuakultur Indonesia Vol. 18 No. 1 (2019): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRAK Motile aeromonad septicaemia (MAS) adalah penyakit yang sering menyerang ikan mas Cyprinus carpio yang disebabkan oleh bakteri Aeromonas hydrophila. Penelitian ini bertujuan untuk menguji kinerja imunostimulan fikosianin dari Spirulina platensis dalam mengatasi penyakit MAS pada ikan mas. Penelitian ini terdiri atas dua tahap, pertama, pakan ikan dengan penambahan fikosianin 150 mg/kg, 250 mg/kg, dan 350 mg/kg pakan serta kontrol tanpa penambahan fikosianin. Setelah 14 hari, ikan diuji tantang dengan A.hydrophila. Tahap kedua, dosis terbaik dari penelitian pertama digunakan untuk pakan ikan masing-masing selama satu minggu/bulan, dua minggu/bulan, tiga minggu/bulan, dan dua minggu/bulan dengan interval satu minggu. Setelah 28 hari, ikan diuji tantang dengan A. hydrophila. Hasil penelitian pertama menunjukkan bahwa kelangsungan hidup relatif (RPS) ikan yang diberi pakan fikosianin 150 mg/kg, 250 mg/kg, dan 350 mg/kg pakan adalah 87,50%; 81,25%; dan 75,00%. Total eritrosit, hemoglobin, total leukosit, aktivitas fagositik, dan respiratory burst menunjukkan hasil yang lebih tinggi daripada kontrol untuk semua perlakuan pemberian fikosianin. Penelitian kedua menunjukkan bahwa nilai RPS ikan diberi pakan selama satu minggu/bulan, dua minggu/bulan, tiga minggu/bulan, dan dua minggu/bulan dengan interval satu minggu yaitu 65,38%; 69,23%; 76,92%; dan 69,23%. Respons imun ikan yang diberi fikosianin lebih tinggi daripada kontrol serta mampu menekan jumlah bakteri A. hydrophila di hati, ginjal, dan usus. Kesimpulan dari penelitian ini bahwa pemberian fikosianin sebanyak 150 mg/kg pakan selama tiga minggu/bulan memiliki nilai RPS tertinggi. Kata kunci: fikosianin, Spirulina platensis, Aeromonas hydrophila, Cyprinus carpio ABSTRACT Motile aeromonad septicaemia (MAS) is a major disease in common carp Cyprinus carpio caused by Aeromonas hydrophila. This study aimed to evaluate the performance of phycocyanin imunostimulant extracted from Spirulina platensis to control MAS disease in common carp. This study was conducted into two phases. First phase was conducted by adding 150 mg/kg, 250 mg/kg, 350 mg/kg feed phycocyanin dose, and 0 mg/kg feed phycocyanin dose as control treatment. Fish was challenged with pathogenic A.hydrophila after 14 days rearing. Second phase was conducted by applying the best dose obtained from the first phase added in the feed for feeding the fish in one week/month, two weeks/month, three weeks /month, and two weeks/month with one week interval. Fish was challenged with pathogenic A.hydrophila after 28 days rearing. First phase study result showed that the relative percent survival (RPS) for fish fed 150 mg/kg, 250 mg/kg, and 350 mg/kg phycocyanin dose were 87.50%, 81.25%, and 75.00% respectively. Total erythrocytes, hemoglobin, total leucocytes, phagocytic activity, and respiratory burst showed higher results than control treatment on all treated fish. The second phase study showed that fish fed one week/month, two weeks/month, three weeks/month, and two weeks/month with one week interval had RPS value 65.38%, 69.23%, 76.92%, and 69.23% respectively. The immune responses of treated fish were higher than control treatment, as well as the number of pathogenic A. hydrophila in the liver, kidney, and intestine. Fish fed with phycoyanin dose 150 mg/kg feed and three weeks/month administration had the highest RPS value. Keywords: Phycocyanin, Spirulina platensis, Aeromonas hydrophila, Cyprinus carpio

Co-Authors , Rahman, , , Ranta, , , Rusli, , . ARIFUDDIN . Maryani . Rahman . Sunarto A. Hasan A. Santika A. Suwanto A.J. Sihombing Ade Dwi Sasanti Aditya, Tiya Widi Afif, Usamah Afiff , Usamah Aldy Mulyadin Alimuddin Alit Brilliant Aminatul Zahra ANGELA MARIANA LUSIASTUTI Angela Mariana Lusiastuti Anis Nugrahawati Annisa Astri Anggraeni Ayi Santika Bambang Gunadi Benny Yustim Brite, Margie Cahyawati, Nadia Catur A. Pebrianto D. Dana D. Meha D. Wahjuningrum Daniel Djokosetiyanto DEBY YUNIASARI Dendi Hidayatullah, Dendi Dewi Rahmi DIAH AYU SATYARI UTAMI Dian Febriani Dinamella Wahjuningrum Dwi Agung Saputra E. Ayuzar E. Harris Eddy Supriyono Eka Angga Laksana Enang Harris Enang Harris Esti Handayani Hardi Evan Farhan Wahyu Puadi F.H. Pasaribu Fachriyan Hasmi Pasaribu Fadilah, Iin Nur Fadlilah, Rizqy Aditya Fitria Novianti Gustilatov, Muhamad Helmy Faisal Muttaqin Henky Manoppo Hisatsugu Wakabayashi I. Normalina I. Nur Iqbal Kurniawinata, Mohamad Irzal Effendi Isni Rahmatika Sari Jasmanindar, Yudiana Jeanni Indah Noermala Julie Ekasari K. Sumantadinata Khairun Nissa Komar Sumantadinata Kukuh Nirmala L. Jamal Lastriliah, Mira LAY, B. W. M. Yuhana M. Zairin Junior M.S. Arifin Mia Setiawati Mochamad Fatuchri Sukadi Muchtar, Muthahharah Muh. Aris Muh. Fatuhcri Sukadi Muharram Nur Ikhsan Mulyani, Rahma MUNTI YUHANA N.A. Maswan Nasri Julaini Nasrullah, Hasan Nur Bambang Priyo Utomo Nuzullia, Laely Odang Carman Ode, Inem P. Hadi Pras, Eva Prasetiyono Pratiwi, Kiki Amalia Putri, Shofii Amaliah R.D. Soejoedono Rafsyanzani, Muhammad Mufthi Rahman Rahman Ramadhani, Dian Eka Ramhirez, Putri Shandra Retno Damayanti Soejoedono Ririn Nurul Fauziah, Ririn Nurul Rizki Praseto, Rizki Rr. Bellya Anasti Maharani Rudi, Mad Ruku Ratu Borut Samsu Adi Rahman Sari Anggraeni, Sukma Septiani, Ghita Ryan Sri Nuryati Sri Nuryati Sugeng Budiharsono Sugiyo Hadi Pranoto Tatag Budiardi Trian Rizky Febriansyah Tsani Untsa, Agista Uba Umbara Ulil Surtia Zulpratita Umbara, Uba Wesly Pasaribu WIDANARNI WIDANARNI Wisriati Lasima Y. Tri Anggoro Yan Evan Yumaidawati, Nurfitriani Siti Yuni Puji Hastuti Zulfani, Anisa

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