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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Akuakultur Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Akuakultur Rawa Indonesia e-Journal BUDIDAYA PERAIRAN ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Ilmu Lingkungan Jurnal Perikanan Kelautan Jurnal Natur Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Ilmu-Ilmu Perikanan dan Budidaya Perairan Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) OCTOPUS : Jurnal Ilmu Perikanan Omni-Akuatika Jurnal Perikanan Universitas Gadjah Mada Jurnal Intek Akuakultur Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Jurnal Kedokteran Hewan Policy Brief Pertanian, Kelautan, dan Biosains Tropika Journal of Fisheries & Marine Biodiversitas Journal of Biological Diversity

Sukenda, Sukenda,

Department Of Aquaculture, Faculty Of Fisheries And Marine Science, Bogor Agricultural University, Bogor

Author-ID : 390256

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Immunology & microbiology Library & Information Science Medicine & Pharmacology Social Sciences Veterinary Other

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Selection of probiotic bacteria from the coral reef and tiger grouper fish (Epinephelus foscoguttatus) farming environment Ilmiah, .; Sukenda, .; Widanarni, .; Harris, Enang
Jurnal Akuakultur Indonesia Vol. 11 No. 2 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The use of probiotic bacteria is one of the methods in aquaculture to control infectious diseases. This study aims to obtain candidates probiotic bacteria from the coral reef and tiger grouper aquaculture environment (Epinephelus fuscoguttatus). Steps of the study included of: 1) isolation of candidates probiotic bacteria, 2) selection of candidates probiotic bacteria: inhibition test and co-culture methods, 3) enzymatic test, 4) the growth of bacteria test 5) pathogenicity test and 6) the molecular identification of probiotic bacteria. The results showed that of 124 isolates in test zone of inhibition, the four isolates with the widest zone of inhibition of isolate K7, K8, K21, and T4, a total of 51 isolates on co-culture methods, the four isolates were able to suppress the growth of pathogenic Vibrio bacteria, isolates K15, K68, K55, and T36. The results of hydrolyzing the highest protease amylase and lipase enzyme, respectively isolates K15 and T36; isolates T41 and K8; isolates K55 and K68. Candidates probiotic bacteria reached the exponential phase at 16‒18 h. The results of pathogenicity test isolate K7, K8, T36, and T41 provided the highest survival that can be used as candidates probiotics in the tiger grouper. Keywords: selection, probiotics, tiger grouper

Protein digestibility and ammonia excretion in catfish Clarias gariepinus culture Gunadi, Bambang; Harris, Enang; Supriyono, Eddy; Sukenda, .; Budiardi, Tatag
Jurnal Akuakultur Indonesia Vol. 12 No. 1 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT A series of experiments was performed to analyze protein digestibility, ammonia excretion, and also heterothropic bacteria and phytoplankton dynamics in the catfish Clarias gariepinus culture. In the digestibility experiment, catfish with an individual initial size of 43.67±0.83 g were stocked into 120 L conical fiberglass tanks at a density of 20 fish per tank. Fish were fed on with commercial diet supplemented with Cr2O3 indicator at a concentration of 1%. In the ammonia excretion experiment, catfish with an individual size of 111.6±9.5 and 40.6±3.4 g, respectively, were placed into a 10 L chamber filled with 8 L of water. Total ammonia nitrogen (TAN) in the chambers were monitored every hour for six consecutive hours. In the bacteria and phytoplankton dynamics experiment, catfish were stocked in the 25 m2 concrete tanks which was divided into two compartments (catfish 10 m2, and heterotrof compartments 15 m2). Catfish with individual size of 42,5±0 g were stocked into the tanks at a density of 100 fish per tank. Water was recirculated from catfish compartments to heterotrophic compartments. Fish were fed with floating feed. Molasses as carbon source for heterotrophic bacteria was applied daily. The experiment was conducted for six weeks. The results showed that the protein digestibility was 61.97±7.24%. Larger fish (size of 111.6 g) excreted ammonia at a rate of 0.008±0.003 mg TAN/g fish-weight/hour, which was lower than that of the smaller catfish (size of 40.6 g), i.e. 0.012±0.004 mg TAN/g fish-weight/hour. Keywords: protein digestibility, ammonia excretion, catfish ABSTRAK Serangkaian penelitian telah dilakukan untuk menganalisis ketercernaan pakan dan protein, ekskresi amonia, serta dinamika bakteri dan fitoplankton pada budidaya ikan lele (Clarias gariepinus). Pada penelitian ketercernaan pakan, ikan lele berukuran 43,67±0,83 g/ekor dipelihara dalam bak fiberglas berbentuk corong berukuran 120 L dengan kepadatan 20 ekor/bak. Ikan diberi pakan berupa pelet yang diberi indikator Cr2O3 sebanyak 1%. Pada penelitian ekskresi amonia, ikan lele berukuran 111,6±9,5 dan 40,6±3,4 g/ekor yang telah diberi makan sampai kenyang dimasukkan ke dalam stoples berisi 8 L air. Kadar amonia total (total ammonia nitrogen, TAN) di dalam stoples diukur setiap jam selama enam jam. Pada penelitian dinamika bakteri dan fitoplankton, ikan lele dipelihara pada bak beton berukuran 25 m2 yang disekat menjadi dua bagian yaitu bagian ikan lele (10 m2) dan bagian heterotrof (15 m2). Ikan lele dengan bobot awal 42,5 g/ekor ditebar ke dalam bak dengan kepadatan 100 ekor/bak. Air mengalir secara resirkulasi dari bagian ikan lele ke bagian heterotrofik dengan bantuan pompa. Pakan yang diberikan berupa pelet apung komersial. Molase ditambahkan setiap hari sebagai sumber karbon untuk pertumbuhan bakteri heterotrofik. Penelitian dilaksanakan selama enam minggu. Hasil pengamatan menunjukkan bahwa ketercernaan protein dari pakan yang diuji adalah 61,97±7,24%. Ikan lele berukuran besar (111,6 g/ekor) menghasilkan amonia sebanyak 0,008±0,003 mg TAN/g ikan/jam, lebih rendah dibandingkan dengan ikan yang berukuran lebih kecil (40,6 g/ekor), yaitu 0,012±0,004 mg TAN/g ikan/jam. Kata kunci: ketercernaan protein, ekskresi amonia, ikan lele

Kappa-carrageenan as immunostimulant to control infectious myonecrosis (IMN) disease in white shrimp Litopenaeus vannamei Febriani, Dian; Sukenda, .; Nuryati, Sri
Jurnal Akuakultur Indonesia Vol. 12 No. 1 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This study evaluated the modulation of non-specific immune response, growth, and disease resistance of white shrimp, Litopenaeus vannamei against infectious myonecrosis virus (IMNV). The first stage of this study evaluated the different dose of k-carrageenan administration i.e. 5, 10, and 15 g/kg feed for four weeks of rearing period, while the later studied evaluated about the frequency of administration i.e. daily, seven days interval, and 14-days interval for five weeks of rearing period. Both stages had positive and negative control and performed in complete randomized design. The parameters of observation consisted of immune parameters, clinical symptoms, growth, and survival. Shrimp were fed three times a day at feeding rate of 4–5% of body weight/day. IMNV Challenge test was performed by feeding the shrimp via oral route at a level 10% of body weight for three consecutive days, followed by 14-days observation. The results showed that shrimp administered with k-carrageenan at a concentration of 15 g/kg feed showed the best performance of all concentration tested. The shrimp’s haemocyte count, phagocytes activity, phenoloxidase activity, and relative growth were 12±0.72×106 cell/mL; 34.67±0.58%; 0.511±0.10; and 86.15% respectively. After challenged, the survival was 85±7.07%. Moreover, application in 14 days at 7-days interval gave 88.57% relative growth and 93±5,8% survival, which were higher than other treatments. The administration of k-carrageenan at concentration of 15 g/kg with 14 days interval on white shrimp juveniles showed higher immunostimulatory effect and better protection against IMNV. Keywords: kappa-carrageenan, immunostimulant, IMNV, Litopenaeus vannamei ABSTRAK Penelitian ini mempelajari pemberian kappa-karagenan dalam memodulasi respons imun nonspesifik, pertumbuhan, dan resistensi udang vaname Litopenaeus vannamei terhadap infeksi infectious myonecrosis virus (IMNV). Tahap pertama mengetahui dosis pemberian k-karagenan sebesar 5, 10, dan 15 g/kg pakan selama empat minggu pemeliharaan, sedangkan tahap kedua mengevaluasi frekuensi pemberian k-karagenan, yaitu setiap hari, tujuh hari, dan 14 hari secara berulang dengan interval tujuh hari selama lima minggu pemeliharaan. Kedua tahap penelitian menggunakan kontrol positif dan negatif dalam rancangan acak lengkap. Parameter pengamatan terdiri atas respons imun, gejala klinis, pertumbuhan dan kelangsungan hidup udang vaname. Udang diberi makan tiga kali sehari dengan FR 4–5% biomassa/hari. Infeksi IMNV dilakukan secara oral sebesar 10% biomassa selama tiga hari berturut-turut, dan diamati selama 14 hari. Hasil penelitian menunjukkan bahwa udang yang diberikan k-karagenan 15 g/kg pakan memperoleh hasil terbaik. Total hemosit, aktivitas fagositosis, aktivitas phenoloxidase, dan pertumbuhan relatif udang masing-masing adalah 12±0,72×106 sel/mL; 34,67±0,58%; 0,511±0,10 dan 86,15%, dengan kelangsungan hidup udang setelah diinfeksi IMNV sebesar 85±7,07%. Frekuensi pemberian 14 hari secara berulang dengan interval tujuh hari memberikan hasil kelangsungan hidup terbaik sebesar 88,57% dan pertumbuhan relatif sebesar 93±5,8%. Kesimpulan dari penelitian ini bahwa, pemberian k-karagenan 15 g/kg dan interval 14 hari menunjukkan respons imun dan perlindungan yang lebih baik terhadap IMNV. Kata kunci: kappa-karagenan, imunostimulan, IMNV, Litopenaeus vannamei

Screening of probiotics bacteria from coral reef using co-culture method for controlling vibriosis in tiger shrimp (Penaeus monodon) larvae Sasanti, Ade Dwi; ., Widanarni; ., Sukenda
Jurnal Akuakultur Indonesia Vol. 10 No. 2 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTThis study was carried out to obtain bacteria isolates from coral reef using co-culture method which potentially inhibit Vibrio harveyi growth. A total of 110 isolates were isolated from Acropora sp., Merulina sp., Hystrix sp., Poecillophora sp., Porites sp., and Haliophora sp., and were screened for their antagonistic activity against V. harveyi in in vitro and in vivo test. Five candidate probiotics (5H1 candidate probiotics isolated from Acropora sp., 11I and 11G isolates isolated from Hystrix sp. and 13B and 13G1 isolates isolated from Poecillophora sp., was able to inhibit growth of V. harveyi MR5339 RFR up to 101‒102 cfu/mL. Two isolates (13B and 13G1) were not pathogenic at concentration 106 cfu/mL bacteria and could increase of survival rate of tiger shrimp (Penaeus monodon) larvae in in vivo test. Survival rate of tiger shrimp larvae that treatment with 13B and 13G1 were 86,67% and 88,33%, and have a significant different with positive control (61,67%). Partial sequencing of 16S-rRNA showed that 13G1 isolate was similar to V. alginolyticus.Keywords: vibriosis, Vibrio harveyi, tiger shrimp, probiotic, coral reefABSTRAKPenelitian ini bertujuan untuk mendapatkan bakteri probiotik asal terumbu karang dengan metode kultur bersama untuk pengendalian penyakit vibriosis pada larva udang windu (Penaeus monodon). Sebanyak 110 isolat berhasil diisolasi dari Acropora sp., Merulina sp., Hystrix sp., Poecillophora sp., Porites sp., dan Heliophora sp. dan dilakukan penapisan untuk melihat aktivitas kemampuannya melawan Vibrio harveyi MR 5339 RfR dalam uji in vitro dan in vivo. Sebanyak 56 isolat menghasilkan daya hambat terhadap V. harveyi MR5339 RfR pada metode kultur bersama. Lima isolat kandidat probiotik (isolate 5H1 diisolasi dari Acropora sp., isolat 11I dan 11G diisolasi dari Hystrix sp., serta isolat 13B dan 13G1 yang diisolasi dari Poecillophora sp.), mampu menekan pertumbuhan V. harveyi MR5339 RfR hingga 101–102 cfu/mL. Kedua isolat (13B dan 13G1) terbukti tidak bersifat patogen pada konsentrasi 106 cfu/mL dan mampu meningkatkan sintasan larva udang windu pada uji in vivo. Nilai sintasan larva pada perlakuan yang diberi kandidat probiotik 13B dan 13G1 berturut-turut adalah 86,67% dan 88,33%, namun berbeda nyata dengan kontrol positif (61,67%). Hasil analisis sekuen sebagian gen 16S-rRNA menunjukkan bahwa isolat 13G1 termasuk spesies V. algynoliticus.Kata kunci: vibriosis, Vibrio harveyi, udang windu, probiotik, terumbu karang

Administration of Phyllanthus niruri to control IMNV (myonecrosis infectious virus) infection white shrimp Litopenaeus vannamei ., Sukenda; Nuryati, Sri; Sari, Isni Rahmatika
Jurnal Akuakultur Indonesia Vol. 10 No. 2 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTInfectious myonecrosis (IMN) disease is a major disease in Indonesia shrimp farming. The disease is caused by infectious myonecrosis virus (IMNV). Currently, treatment and drug has not been obtained to control the virus. This research was conducted to determine the effect of Phyllanthus niruri extract in white shrimp (Litopenaeus vannamei) against IMNV infection. Healthy shrimp was given P. niruri extract 20 mg/kg of feed for seven days and after that the shrimp was challenged by orally with IMNV infected shrimp tissue. The positive control was given feed without P. niruri extract and challenged with IMNV infected shrimp tissue, while negative control was not challenged with IMNV infected shrimp tissue. IMNV infection gave a significantly different effect on survival rate. In the shrimp P. niruri previously (86.7%) gave higher survival rate compared to shrimp without P. niruri (66.67%). Survival rate of negative control was 93.33%. IMNV clinical signs in general was white necrotic areas in striated muscles. Histological examination showed that cell necrosis appeared on the mussel tissues. In conclusion the addition of P. niruri to the commercial feed can give the survival rate of shrimp better when challenged with IMNV.Keywords: IMNV, Phyllanthus niruri, Litopenaeus vannameiABSTRAKPenyakit infectious myonecrosis (IMN) merupakan penyakit utama pada budidaya udang di Indonesia. Penyakit ini disebabkan oleh infectious myonecrosis virus (IMNV). Saat ini, belum diperoleh cara dan obat untuk mengendalikan virus IMNV. Penelitian ini dilakukan untuk mengevaluasi pengaruh immunostimulan tepung meniran (Phyllanthus niruri) yang diberikan melalui pakan pada udang vaname (Litopenaeus vannamei) yang diinfeksi IMNV. Udang vaname yang sehat diberi pakan yang mengandung meniran dengan dosis 20 mg/kg pakan selama tujuh hari dan kemudian diuji tantang secara oral dengan memberikan jaringan udang yang telah terinfeksi IMNV. Udang kontrol positif dilakukan dengan memberi pakan komersial tanpa penambahan meniran yang kemudian diuji tantang dengan memberi makan jaringan udang yang terinfeksi IMNV, sedangkan udang kontrol negatif tidak diuji tantang dengan jaringan yang terinfeksi IMNV. Hasil menunjukkan bahwa kelompok udang yang diberi pakan mengandung meniran mempunyai kelangsungan hidup (86,67%) lebih tinggi dibandingkan dengan udang yang tidak diberi pakan mengandung meniran (66,67%) ketika diuji tantang dengan IMNV. Kontrol negatif yang tidak diberi pakan mengandung meniran dan tidak diuji tantang dengan IMNV memberikan kelangsungan hidup 93,33%. Gejala klinis yang ditunjukkan adanya infeksi IMNV terlihat dengan adanya otot putih pada ruas tubuh udang. Pengamatan histopatologi menunjukkan adanya nekrosis pada sel-sel di jaringan otot udang. Sebagai kesimpulan dapat dinyatakan bahwa penambahan meniran pada pakan komersial dapat meningkatkan kelangsungan hidup udang ketika terjadi infeksi IMNV.Kata kunci: IMNV, Phyllanthus niruri, Litopenaeus vannamei

Application of synbiotic with different probiotic doses to prevent vibriosis in humpback grouper Saputra, Dwi Agung; Sukenda, ,; Widanarni, ,
Jurnal Akuakultur Indonesia Vol. 12 No. 2 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The aim of this study was to evaluate the supplementation of synbiotic with different doses of probiotics to prevent vibriosis in humpback grouper (Cromileptes altivelis). Grouper (3.0±0.48 g) fed by control feeds (without supplementation of synbiotic) which were K(-) and K(+), treatment feeds with supplementation of synbiotic with different doses of probiotic (probiotic 104 cfu/mL) 1% + prebiotic 2% (P1) v/w, (probiotic 106 cfu/mL) 1% + prebiotic 2% (P2) v/w, (probiotic 108 cfu/mL)1% + prebiotic 2% (P3) v/w) for 30 days. After a feeding trial period, there were observation of the bacterial counts in the fish intestine, the fish growth performance and immune response. Then all the grouper were challenged by Vibrio alginolyticus, except K (-). This study showed that survival, daily growth and food conversion ratio (FCR) of grouper in treatment P2 ((probiotic 106 cfu/mL) 1% + prebiotic 2%) and P3 (probiotic (108 cfu/mL) 1% + prebiotic 2%) were significantly better (P>0.05) than controls. The different doses of probiotic in synbiotic (probiotic 104 cfu/mL, probiotic 106 cfu/mL and probiotic 108 cfu/mL) provided better immune response than controls. Keywords: synbiotic, Vibrio alginolyticus, Bacillus sp., Cromileptes altivelis ABSTRAK Tujuan penelitian ini adalah mengkaji pemberian sinbiotik dengan dosis probiotik berbeda untuk mencegah penyakit vibriosis pada ikan kerapu bebek (Cromileptes altivelis). Ikan kerapu dengan berat (3,0±0,48 g) diberikan pakan kontrol (tanpa penambahan sinbiotik) K(-) dan K(+), pakan perlakuan dengan penambahan sinbiotik dengan dosis probiotik berbeda: probiotik ((104 cfu/mL) 1% + prebiotik 2%) v/w (P1), ((probiotik 106 cfu/mL) 1% + prebiotik 2%) v/w (P2), dan ((probiotik 108 cfu/mL)1% + prebiotik 2%) v/w (P3) selama 30 hari. Setelah perlakuan pakan sinbiotik, dilakukan pengamatan terhadap jumlah total bakteri di usus, kinerja pertumbuhan dan respons imun. Kemudian ikan kerapu pada seluruh perlakuan, kecuali kontrol negatif (-) diberi uji tantang dengan Vibrio alginolyticus. Hasil penelitian menunjukkan bahwa sintasan, laju pertumbuhan harian (LPH), dan rasio konversi pakan (FCR) pada perlakuan P2 ((probiotik 106 cfu/mL) 1% + prebiotik 2%) dan P3 (probiotik (108 cfu/mL) 1% + prebiotik 2%) secara signifikan lebih baik (P<0,05) bila dibandingkan dengan kontrol. Perlakuan sinbiotik dengan dosis probiotik berbeda (probiotik 104 cfu/mL, probiotik 106 cfu/mL, dan probiotik 108 cfu/mL) juga memberikan respons imun yang lebih baik dibandingkan dengan kontrol. Kata kunci : sinbiotik, Vibrio alginolyticus, Bacillus sp., Cromileptes altivelis

The use of Curcuma longa extract to control Edwardsiella tarda infection on Clarias sp. Wahjuningrum, Dinamella; Ikhsan, Muharram Nur; Sukenda, ,; Evan, Yan
Jurnal Akuakultur Indonesia Vol. 13 No. 1 (2014): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Target production of catfish in aquaculture can be reached by suppressing the pathogenic bacterial infection. Previous works had shown that turmeric Curcuma longa has antibacterial activity. The objectives of this research were to determine the best method of extraction and to evaluate the efficacy of turmeric extract as feed additive to control Edwardsiella tarda disease in catfish culture. Briefly, the objective was achieved through in vitro assay based on inhibition ability of extraction method against E. tarda, while the following objective was obtained through in vivo assay based on their survival during challenge test either as preventive or curative measurement. A complete randomized design with three replications was used for each assay. Furthermore, challenge test was done by mean intraperitoneal injection at concentration 106 cfu/mL of E. tarda (LD50). The results showed that 15 minutes decoction method allowed the best inhibition with diameter 7.42 mm of clear zone and then curative measurement using turmeric extract could be the best application against E. tarda since it gave 86.67% of survival. Clinical signs such as swelling, hemoraghic, body ulcer, gastroentritis and gaseous captivity were observed on challenged fish. However, there were no significant different among treatments for specific growth, body weight, and absolute length parameters. Keywords: Edwardsiella tarda, extraction, turmeric, catfish, inhibition zone ABSTRAK Peningkatan produksi ikan lele dapat dicapai melalui pencegahan infeksi penyakit bakterial. Hasil beberapa penelitian membuktikan bahwa kunyit Curcuma longa terbukti memiliki zat aktif yang bersifat antibakteri. Penelitian ini bertujuan untuk mencari metode ekstraksi terbaik dan mengevaluasi efektivitas penambahan ekstrak kunyit pada pakan untuk pengendalian patogen Edwardsiella tarda pada ikan lele. Metoda ekstraksi kunyit diuji secara in vitro dengan metoda zona hambat, sedangkan efikasi diuji secara in vivo melalui perlakuan pencegahan dan pengobatan. Penelitian didesain dalam rancangan acak lengkap dengan tiga ulangan. Efikasi ekstrak kunyit diketahui dari nilai sintasan ikan lele hasil uji tantang E. Tarda melalui injeksi peritoneal dengan dosis 106 cfu/ mL (LD50). Hasil uji in vitro menunjukkan bahwa metode dekoksi selama 15 menit memberikan zona hambat terbaik yaitu sebesar 7,42 mm. Hasil uji in vivo menunjukkan bahwa ekstrak kunyit sebagai tindak pengobatan memberikan nilai sintasan terbaik, yaitu sebesar 86,67%. Ikan lele yang diuji tantang menunjukkan gejala klinis berupa pembengkakan, luka, gastroentritis, dan gas pada perut. Tidak terdapat perbedaan nyata di antara perlakuan untuk parameter laju pertumbuhan harian, bobot harian, dan pertumbuhan panjang mutlak. Kata kunci: Edwardsiella tarda, ekstraksi, kunyit, ikan lele, zona hambat

Prebiotic, probiotic, and synbiotic to control Vibrio harveyi and IMNV co-infection in Litopenaeus vannamei Widanarni, ,; Noermala, Jeanni Indah; Sukenda, ,
Jurnal Akuakultur Indonesia Vol. 13 No. 1 (2014): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This study aimed to examine the effects of prebiotic, probiotic, and synbiotic in survival and immune response of white shrimp against co-infection of Vibrio harveyi and infectious myonecrosis virus (IMNV). The shrimps used had a body weigth of 2.04±0.20 g/individual, the shrimps were reared at a density of 20 shrimps in 60x30x35 cm3 sized aquarium. The study was conducted with five treatments consisting K(+) (without the addition of prebiotic, probiotic, and synbiotic with co-infection), K(-) (without the addition of prebiotic, probiotic and synbiotic, and without co-infection), P1 (the addition of prebiotic with co-infection), P2 (the addition of probiotic with co-infection), and P3 (the addition of synbiotic with co-infection). The results showed that the addition of prebiotic, probiotic and synbiotic could increase survival and immune response of white shrimp towards co-infection of Vibrio harveyi and IMNV. The best survival was obtained in probiotic treatment (79.17%), followed by prebiotic treatment (75%), synbiotic treatment (70.83%), while the positive control was only 50%. Keywords: white shrimp, prebiotic, probiotic, synbiotic, IMNV, Vibrio harveyi ABSTRAK Penelitian ini bertujuan untuk menguji pengaruh pemberian prebiotik, probiotik, dan sinbiotik terhadap sintasan dan respons imun udang vaname dengan ko-infeksi Vibrio harveyi dan IMNV (infectious myonecrosis virus). Udang yang digunakan memiliki bobot 2,04±0,20 g/ekor, dipelihara sebanyak 20 ekor dalam akuarium berukuran 60x30x35 cm3. Penelitian dilakukan dengan lima perlakuan yaitu K(+) (tanpa pemberian prebiotik, probiotik, dan sinbiotik dengan ko-infeksi), K(-) (tanpa pemberian prebiotik, probiotik dan sinbiotik tanpa ko-infeksi), P1 (pemberian prebiotik dengan ko-infeksi), P2 (pemberian probiotik dengan ko-infeksi), dan P3 (pemberian sinbiotik dengan ko-infeksi). Hasil penelitian menunjukkan bahwa pemberian prebiotik, probiotik, dan sinbiotik mampu meningkatkan sintasan dan respons imun udang vaname terhadap ko-infeksi V. harveyi dan IMNV. Sintasan terbaik diperoleh pada perlakuan probiotik sebesar 79,17%, diikuti perlakuan prebiotik sebesar 75%, perlakuan sinbiotik sebesar 70,83%, sedangkan pada kontrol positif hanya mencapai 50%. Kata kunci: udang vaname, prebiotik, probiotik, sinbiotik, IMNV, Vibrio harveyi

Whole-cell vaccine of Streptococcus agalactiae in Oreochromis sp. with immersion method Sukenda, ,; Febriansyah, Trian Rizky; Nuryati, Sri
Jurnal Akuakultur Indonesia Vol. 13 No. 1 (2014): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The study was aimed to evaluate the efficacy of formalin-killed non-hemolytic Streptococcus agalactiae N14G and NK1 isolates whole-killed vaccine to prevent streptococcosis in tilapia. Ten fishes were reared in a tank 60x30x35 cm3 with an average body weight at 10.79±0.99 g. Fish was vaccinated through bath immersion at a concentration of 109 cfu/mL. Fish was subsequently challenged by intraperitonial injection of Streptococcus agalactiae 105 cfu/mL at 11 days post-vaccination. Parameters observed were survival, relative percent survival (RPS), total leukocyte, phagocytic activity, antibody titer, total erythrocyte, haemoglobin level, haematocrit level, dan water quality. Samplings were performed in day-0, 20, and 30 after vaccination. Both vaccines have shown higher survival (60%) and RPS (40%) when challenged with pathogenic Streptococcus N14G isolates than other treatments. Based on RPS percentage observed, those vaccine were still not sufficiently effective to combat S. agalactiae infection. Keywords: tilapia, bath immersion, Streptococcus agalactiae, whole-cell vaccine ABSTRAK Penelitian ini bertujuan untuk mengevaluasi efikasi vaksin formalin-killed cell Streptococcus agalactiae tipe isolat nonhemolitik N14G dan NK1 se utuh yang diberikan melalui perendaman dalam mencegah penyakit streptococcosis pada ikan nila. Ikan nila yang digunakan memiliki bobot 10,79±0,99 g, dipelihara sebanyak sepuluh ekor dalam akuarium ukuran 60x30x35 cm3. Ikan divaksinasi dengan metode perendaman dengan dosis 109 cfu/mL. Uji tantang dilakukan pada hari ke-11 pascavaksinasi dengan dosis 105 cfu/mL. Parameter yang diamati meliputi sintasan (SR), sintasan relatif/relative percent survival (RPS), total leukosit, aktivitas fagositik, titer antibodi, total eritrosit, kadar hemoglobin, kadar hematokrit, dan kualitas air. Pengamatan parameter dilakukan pada hari ke-0, ke-10, ke-20, dan ke-30. Hasil penelitian menunjukkan perlakuan kedua vaksin yang diinfeksi bakteri patogen isolat N14G memberikan nilai sintasan dan nilai RPS tertinggi dibanding perlakuan lainnya. Nilai sintasan dan RPS kedua perlakuan tersebut adalah 60% dan 40%. Nilai RPS yang cukup kecil menunjukkan vaksin yang diberikan masih kurang efektif untuk mencegah infeksi bakteri S. agalactiae. Kata kunci: ikan nila, perendaman, Streptococcus agalactiae, vaksin sel utuh

Effectiveness of sinbiotic at different doses in Pacific white shrimp pond culture Sukenda, ,; Praseto, Rizki; Widanarni, ,
Jurnal Akuakultur Indonesia Vol. 14 No. 1 (2015): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The increasing demand of white shrimp Litopenaeus vannamei requires the application of intensive culture system. However, intensive culture system of white shrimp could increase the risk of disease outbreak. The application of sinbiotic may provide solution to the problem. This study was aimed to evaluate the effectiveness of the use of technical sinbiotic on the survival and growth of white shrimp in ponds. This study consisted of four treatments; treatment K (control), treatment A (probiotic 0.5% and prebiotic 1%), treatment B (probiotic 1% and prebiotic 2%), and treatment C (probiotic 2% and prebiotic 4%). The results showed that administration of sinbiotic had no significant differences on survival rate, growth rate, feed conversion ratio, size, and biomass of shrimp (P>0.05). However, based on analysis of business, sinbiotic A provided higher profits to the farmer (Rp10.230) compared to other symbiotic treatments and control. Keywords: Litopenaeus vannamei, sinbiotic, technical media ABSTRAK Permintaan terhadap udang vaname Litopenaeus vannamei yang semakin meningkat membuat sistem budidaya udang sebaiknya menggunakan sistem budidaya intensif. Namun demikian sistem intensif pada budidaya udang vaname dapat meningkatkan risiko timbulnya penyakit. Penggunaan sinbiotik diharapkan dapat memberikan solusi dalam mengatasi masalah tersebut. Penelitian ini bertujuan untuk menguji efektivitas penggunaan sinbiotik teknis terhadap sintasan, pertumbuhan, dan keuntungan usaha udang vaname yang dipelihara di tambak. Penelitian ini terdiri atas empat perlakuan, yaitu perlakuan K (kontrol), perlakuan A (probiotik 0,5% dan prebiotik 1%), perlakuan B (probiotik 1% dan prebiotik 2%), dan perlakuan C (probiotik 2% dan prebiotik 4%). Hasil penelitian menunjukkan bahwa pemberian sinbiotik tidak berbeda nyata dalam nilai sintasan, laju pertumbuhan, rasio konversi pakan, size, dan biomassa udang (P>0,05). Namun demikian, berdasarkan analisis usaha perlakuan A memberikan keuntungan lebih tinggi (Rp10.230) dibandingkan dengan perlakuan sinbiotik lainnya serta kontrol. Kata kunci: Litopenaeus vannamei, sinbiotik, media teknis

Co-Authors , Rahman, , , Ranta, , , Rusli, , . ARIFUDDIN . Maryani . Sunarto A. Hasan A. Santika A. Suwanto A. SUWANTO A.J. Sihombing ADE DWI SASANTI Ade Dwi Sasanti Aditya, Tiya Widi Afif, Usamah Afiff , Usamah Agung Cahyo Setyawan Ahmad Beni Rouf Aldy Mulyadin Alimuddin Alimuddin Alimuddin Alit Brilliant Aminatul Zahra Amrullah Amrullah Andi Parenrengi Andi Parenrengi Andi Tenriulo Andi Tenriulo Angela M Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti ANGELA MARIANA LUSIASTUTI Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Anis Nugrahawati Annisa Astri Anggraeni Ardana Kurniaji Ayi Santika Azis Azis B. W. LAY Bambang Gunadi Bambang Gunadi Brite, Margie Cahyawati, Nadia Catur A. Pebrianto D. Dana D. Meha D. Wahjuningrum Daniel Djokosetiyanto Daniel Djokosetiyanto Daniel Djokosetiyanto DEBY YUNIASARI Dendi Hidayatullah Dendi Hidayatullah Dendi Hidayatullah Dendi Hidayatullah Dendi Hidayatullah, Dendi Desy Sugiani Desy Sugiani Dewi Rahmi DIAH AYU SATYARI UTAMI Dian Febriani Dinamella Wahjuningrum Dwi Agung Saputra E. Ayuzar E. Harris Eddy Supriyono Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Surawidjaja Enang Harris Surawidjaja Endang Susianingsih Endang Susianingsih Esti Handayani Hardi Eva Prasetiyono Eva Prasetiyono Pras F.H. Pasaribu Fachriyan Hasmi Pasaribu Fadilah, Iin Nur Fadlilah, Rizqy Aditya Fahmi Rajab Firmansyah, Arif Lukman Fitria Novianti Ghita Ryan Septiani Gustilatov, Muhamad Henky Manoppo Henky Manoppo Hisatsugu Wakabayashi Huda Salahudin Darusman Huda Shalahudin Darusman I Wayan Nurjaya I. Normalina I. Nur I. Tepu Ibnu Bangkit Bioshina Suryadi Ince Ayu Khairana Kadriah Ince Ayu Khairani Kadriah Inem Ode Iqbal Kurniawinata, Mohamad Irzal Effendi Isni Rahmatika Sari Jasmanindar, Yudiana Jean-Christophe Avarre Jeanni Indah Noermala Julie Ekasari K. Sumantadinata Khairun Nissa Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Kukuh Nirmala Kukuh Nirmala L. Jamal Lastriliah, Mira M. Yuhana M. Zairin Junior M.S. Arifin MIA SETIAWATI Mia Setiawati Mochamad Fatuchri Sukadi Mochamad Fatuchri Sukadi Muchtar, Muthahharah Muh. Aris Muh. Fatuhcri Sukadi MUHAMMAD AGUS SUPRAYUDI Muhammad Yamin Muhammad Zairin Jr. Muhammad Zairin Jr. Muharram Nur Ikhsan Mulyani, Rahma MUNTI YUHANA N.A. Maswan Nasri Julaini Nasrullah, Hasan Nunak Nafiqoh Nunuk Listiyowati Nur Bambang Priyo Utomo Nurbariah Nurbariah Nuzullia, Laely Odang Carman Ode, Inem P. Hadi Pratiwi, Kiki Amalia Priadi Setyawan Putri, Shofii Amaliah R.D. Soejoedono Rafsyanzani, Muhammad Mufthi Rahma Mulyani Rahman Rahman Ramadhani, Dian Eka Ramhirez, Putri Shandra Retno Damayanti Soejoedono RIDWAN AFFANDI Ririn Nurul Fauziah, Ririn Nurul Rizki Praseto, Rizki Ronald Kriston Sauttua Nainggolan Rr. Bellya Anasti Maharani Rudi, Mad Ruku Ratu Borut Samsu Adi Rahman Sari Anggraeni, Sukma Sefti Heza Dwinanti, Sefti Heza Septiani, Ghita Ryan Siti Subaidah Sri Nuryati Sri Nuryati Sugeng Budiharsono Sugiyo Hadi Pranoto Suhermanto, Achmad Tatag Budiardi Tatik Mufidah Tatik Mufidah Trian Rizky Febriansyah Tsani Untsa, Agista Tuti Sumiati Tuti Sumiati Wesly Pasaribu Wida Lesmanawati Widanarni WIDANARNI WIDANARNI WIDANARNI WIDANARNI WIDANARNI WIDANARNI Wisriati Lasima Wiyoto Wiyoto Woro Nur Endang Sariati Y. Hadiroseyani Y. Tri Anggoro Yan Evan Yosmaniar Yosmaniar Yosmaniar Yosmaniar Yumaidawati, Nurfitriani Siti Yuni Puji Hastuti Zulfani, Anisa

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