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Isolation of Lytic Bacteriophages infected Indonesian-strain Vibrio parahaemolyticus and its Protective Effects on Brine Shrimp (Artemia sp.) Wahjuningrum, Dinamella; Ramhirez, Putri Shandra; Nuzullia, Laely; Yuhana, Munti; Sukenda, Sukenda; Nasrullah, Hasan
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 1 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.vi.67419

Abstract

Graphical Abstract Highlight Research vB_Vp_TSK01 and vB_Vp_JKT02 a specific parahaemolyticus bacteriophage were isolated from the shrimp aquaculture water. Compared to control vB_Vp_TSK01 reduced 11.46±1.35% of bacterial growth, and by 9.86±5.92% for vB_Vp_JKT02 treatment. Their cocktail had the highest parahemolyticus growth inhibition by 30.92±3.89%. The phage treatment increased the survival of the Artemia by 28.57% compared to infection control.     Abstract Acute Hepatopancreatic Necrosis Disease (AHPND) caused by V. parahaemolyticus infection was one of the major diseases in shrimp culture in recent years. The Vibrio could also affect the survival of Artemia as the shrimp’s main live feed in the hatchery and they become the possible carrier for the AHPND. Phage therapy in shrimp aquaculture could reduce the application of the antibiotic as an antibacterial agent for the AHPND. The present study aimed to isolate the specific lytic phage for the Indonesian strain of V. parahaemolyticus (Vp) and evaluate the phage therapy for the brine shrimp Artemia infected with the Vp. The Vp-specific phage was isolated from the shrimp farm’s water at Tasikmalaya, and North Jakarta City, Indonesia. After isolation and plaque assay, brine shrimp were used as a model to evaluate the phages’ anti-Vibrio activity The Vp-lytic phage was successfully isolated from shrimp culture water at North Jakarta and Tasikmalaya (Vb_Vp_TSK01 and Vb_Vp_JKT01, respectively) and the results showed that both isolated phages and their cocktails were capable to inhibit the growth of Vp with the highest inhibition shown at the cocktail treatment (p<0.05). The survival of Artemia was higher in the phage treatments (p<0.005) compared to the infected control. Infected control had 68.33% of brine shrimp survival, and the Vb_Vp_TSK01, Vb_Vp_JKT01, and their cocktail had similar average brine shrimp survival of 91.11%. In conclusion, phage therapy proved effective in preventing vibriosis in brine shrimp under the conditions tested.
APLIKASI SINBIOTIK UNTUK PENCEGAHAN INFEKSI INFECTIOUS MYONECROSIS VIRUS PADA UDANG VANAME (Litopenaeus vannamei) (Synbiotic Application for Prevention of Infectious Myonecrosis Virus Infection in White Shrimp (Litopenaeus vannamei)) Widanarni, Widanarni; Sukenda, Sukenda; Septiani, Ghita Ryan
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5041

Abstract

This study aimed to evaluate the effectiveness of dietary synbiotic at different giving frequencies on growth, immune responses, and resistance of white shrimp infected by infectious myonecrosis virus (IMNV). Synbiotic used in this study was combination of probiotic Vibrio alginolyticus SKT-b and prebiotic oligosaccharides extracted from sweet potatoe (Ipomoea batatas L). Doses of probiotic and prebiotic used were 1% and 2% (w/w), respectively. The white shrimps (0.4930.035 g) were divided into five treatments consisting of A and B (without supplementation of synbiotic: (A) positive control; (B) negative control), C (daily synbiotic supplementation), D (twice a week synbiotic supplementation), and E (weekly synbiotic supplementation). After 30 days of feeding trial, white shrimps were infected by IMNV (except negative control). The results showed that daily growth rate of white shrimp on all synbiotic treatments (C, D, and E) ranged from 6.930.025-6.970.019% and had higher values than controls (A and B) (P0.05). Meanwhile, feed conversion value in C and D (1.540.142 and 1.580.117) were lower than controls (P0.05). Supplementation of synbiotic with different frequencies also affected survival rate of white shrimp after the challenge test with IMNV; daily synbiotic supplementation (C) resulted in a 50% higher survival rate than positive control (P0.05). This was associated with immune responses parameters values of synbiotic treatment (before and after the challenge test) which were better than positive control. In conclusion the addition of synbiotic in feed resulted in higher growth performances, immune responses,and resistance of white shrimp to IMNV infection.
EVALUASI SISTEM PRODUKSI UDANG VANAME Litopenaeus vannamei SAAT TERJADI WABAH PENYAKIT IMNV DI TAMBAK UDANG PINANG GADING, BAKAUHENI, LAMPUNG SELATAN Hidayatullah, Dendi; Fadlilah, Rizqy Aditya; Sukenda, Sukenda; Effendi, Irzal
OCTOPUS: JURNAL ILMU PERIKANAN Vol. 13 No. 1 (2024): OCTOPUS
Publisher : Universitas Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26618/o.v13i1.15027

Abstract

Udang vaname (Litopennaeus vannamei) merupakan salah satu komoditas unggulan budidaya perairan di Indonesia. Penyakit udang merupakan salah satu tantangan yang dihadapi oleh pembudidaya. Salah satu penyakit yang menyerang udang adalah infectious myonecrosis (IMN). Tujuan penelitian ini adalah mengevaluasi sistem produksi budidaya udang vaname saat terjadi wabah infeksi infectious myonecrosis virus IMNV di Tambak Pinang Gading, Bakauheni, Lampung Selatan. Sebanyak 2 petak tambak udang digunakan sebagai petak pengamatan yaitu petak A7 (2700 m2) dan A8 (3700 m2) dengan padata tebar udang masing-masing 74 ekor/m2 dan 54 m2. Kegiatan budidaya udang dilakukan mulai dari persiapan hingga panen. Penelitian dilakukan dengan mengevaluasi kinerja produksi dan kelayakan usaha budidaya udang vaname. Hasil penelitian menunjukkan bahwa  budidaya udang pada kondisi baik, kelangsungan hidup udang dapat mencapai 94,75% dengan keuntungan Rp. 43.335.000/tahun dan kondisi tidak baik saat terserang IMNV mencapai 34,83% dengan kerugian Rp. 49.906.080/tahun. Oleh karena itu, dapat disimpulkan bahwa budidaya udang vaname memiliki peluang usaha yang baik namun juga memiliki resiko kerugian akibat penyakit seperti IMNV. Evaluasi standar operasional prosedur (SOP) dan peningkatan biosekutitas perlu dilakukan untuk menjaga agar produksi budidaya udang tetap baik dan berkelanjutan.
ADMINISTRATION OF Curcuma spp. EXTRACT TO CONTROL Aeromonas hydrophila INFECTION IN STRIPED CATFISH (Pangasianodon hypophthalmus) Sasanti, Ade Dwi; Widanarni, Widanarni; Sukenda, Sukenda; Yuhana, Munti; Wahjuningrum, Dinamella; Setiawati, Mia
Indonesian Aquaculture Journal Vol 20, No 1 (2025): (June, 2025)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.20.1.2025.23-36

Abstract

Curcuma spp. is a phytobiotic with potential application in fish farming. This study aimed to evaluate the potential of Curcuma zedoaria, Curcuma aeruginosa and Curcuma mangga extracts in striped catfish infected with Aeromonas hydrophila. The study used a complete randomized design (CRD) consisting of six treatments: KN (negative control), KP (positive control, fish infected without treatment), PE (C. zedoaria extract 6.25 g kg-1), HE (C. aeruginosa extract 6.25 g kg-1), ME (C. mangga extract 6.25 g kg-1) and KE (combination of C. zedoaria extract 2.1 g kg-1 + C. aeruginosa extract 2.1 g kg-1 + C. mangga extract 2.1 g kg-1). A total of 360 striped catfish, 10 ± 0.5 cm were kept in 18 aquariums measuring 50 × 40 × 35 cm3 and fed the treatment for 30 days. The challenge test was conducted on day 31 by injecting A. hydrophila suspension (106 colony-forming units (CFU) mL-1) intramuscularly into fish. The results showed that the treatment administered were able to stimulate the expression of interleukin-1â, interferon-ã 2a, 2b genes, increase the number of red and white blood cells, hematocrit, hemoglobin, phagocytosis activity, respiratory burst, reduce the population of A. hydrophila in the intestine, and reduce tissue damage in striped catfish. Striped catfish treated with the extracts showed significantly higher survival rates (p<0.05) compared to the positive control group. The survival rates were: KN (100.00 %), KP (53.33%), PE (93.33 %), HE (91.67 %), ME (93.33 %), and KE (88.33 %). In conclusion, the treatment administered were able to enhance the immune response and resistance of striped catfish infected with A. hydrophila.
EFFECT OF NEGATIVE REDOX POTENTIAL OF DRY SEDIMENT ON THE INFECTIVITY OF Vibrio parahaemolyticus, GROWTH AND HEALTH STATUS OF PACIFIC WHITELEG SHRIMP Putri, Shofii Amaliah; Sukenda, Sukenda; Yuhana, Munti; Nirmala, Kukuh
Indonesian Aquaculture Journal Vol 20, No 2 (2025): (December, 2025)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.20.2.2025.133-144

Abstract

Sediment serves as a site for the accumulation of aquaculture waste. Oxidation-Reduction Potential (ORP) is an index indicating anaerobic conditions. A combination of host, pathogen, and suboptimal environmental quality can lead to disease outbreaks. One such disease is Acute Hepatopancreatic Necrosis Disease (AHPND), caused by Vibrio parahaemolyticus. This study was aimed to analyse the impact of sediment drying on infectivity of V. parahaemolyticus growth and health status of Pacific whiteleg shrimp. The sediment ORP used at the initial study was -37 mV. The sediment was dried for 120 hours at a temperature of 40.75 ± 2.73 °C. A total of 15 shrimp/aquarium with an average body weight of 2.35±0.22 g/individual were reared in aquarium sized 20x25x30 cm3 containing dried and undried sediment for 120 hours, then contaminated with 105 CFU/mL V. parahaemolyticus through immersion from the start of rearing until 20 days later. The results showed that sediment drying was able to reduce bacterial abundance and increase ORP sediment. The longer duration of cultivation, the more the sediment ORP decreased. Sediment ORP decreased with increasing shrimp farming duration. The highest bacterial abundance in the sediment on thrid phase of study was observed in the positive control treatment on D+10 ((2.02±0.39)x104 CFU/g), then it decreased until the end. Meanwhile, bacterial abundance in water and hepatopancreas decreased until the end of the study. Survival rates, immune responses, and growth parameters of shrimp with sediment drying were higher than the positive control (p<0.05) but not significantly different from the negative control. The histopathology of shrimp hepatopancreas profile in the sediment drying treatment was better compared to the positive control.
RESPONS IMUN UDANG VANAME PADA SISTEM BIOFLOK DENGAN KEPADATAN BERBEDA TERHADAP INFEKSI Vibrio parahaemolyticus Widanarni, Widanarni; Fadilah, Iin Nur; Cahyawati, Nadia; Yuhana, Munti; Sukenda, Sukenda; Gustilatov, Muhamad
Media Akuakultur Vol 20, No 2 (2025): Desember 2025
Publisher : Politeknik Kelautan dan Perikanan Bone

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/ma.20.2.2025.51-61

Abstract

Budidaya udang vaname (Litopenaeus vannamei) pada kepadatan tinggi meningkatkan stres dan risiko infeksi bakteri, khususnya Vibrio parahaemolyticus. Teknologi bioflok (BFT) melalui penambahan karbon  organik dapat meningkatkan biomassa mikroba heterotrof yang memperbaiki respons imun dan menurunkan virulensi bakteri. Penelitian ini bertujuan menganalisis respons imun dan resistansi udang yang dipelihara dengan padat tebar berbeda dalam sistem bioflok terhadap infeksi V. parahaemolyticus. Udang (0,44±0,02 g) dipelihara selama 7 hari sebanyak 16 perlakuan hasil kombinasi sistem bioflok dan kontrol (tanpa bioflok) dengan kepadatan 4, 8, 12, dan 16 ekor L-¹, lalu diuji tantang melalui perendaman dengan dosis V. parahaemolyticus 10u CFU mL-¹ dimulai sejak awal perlakuan. Hasil menunjukkan bahwa sistem bioflok meningkatkan parameter imun udang, termasuk total hemocyte count (THC), aktivitas fagositik (AF), respiratory burst (RB), dan aktivitas phenoloxidase (PO) secara signifikan (P<0,05) dibandingkan kontrol. Kelimpahan V. parahaemolyticus di air dan tubuh udang perlakuan bioflok lebih rendah dan berbeda signifikan (P<0,05) dengan kontrol positif. Bioflok juga meningkatkan tingkat kelangsungan hidup, dengan nilai pada kepadatan 4 ekor L-¹ (83,33±5,77%), dan tetap lebih baik dibandingkan kontrol hingga kepadatan 16 ekor L-¹. Sistem bioflok terbukti efektif meningkatkan respons imun, menekan infeksi V. parahaemolyticus, dan mempertahankan kelangsungan hidup udang vaname pada kepadatan tinggi.
Co-Authors , Rahman, , , Ranta, , , Rusli, , . ARIFUDDIN . Maryani . Rahman . Sunarto A. Hasan A. Santika A. Suwanto A.J. Sihombing Ade Dwi Sasanti Aditya, Tiya Widi Afif, Usamah Afiff , Usamah Aldy Mulyadin Alimuddin Alit Brilliant Aminatul Zahra ANGELA MARIANA LUSIASTUTI Angela Mariana Lusiastuti Anis Nugrahawati Annisa Astri Anggraeni Ayi Santika Bambang Gunadi Benny Yustim Brite, Margie Cahyawati, Nadia Catur A. Pebrianto D. Dana D. Meha D. Wahjuningrum Daniel Djokosetiyanto DEBY YUNIASARI Dendi Hidayatullah, Dendi Dewi Rahmi DIAH AYU SATYARI UTAMI Dian Febriani Dinamella Wahjuningrum Dwi Agung Saputra E. Ayuzar E. Harris Eddy Supriyono Eka Angga Laksana Enang Harris Enang Harris Esti Handayani Hardi Evan Farhan Wahyu Puadi F.H. Pasaribu Fachriyan Hasmi Pasaribu Fadilah, Iin Nur Fadlilah, Rizqy Aditya Fitria Novianti Gustilatov, Muhamad Henky Manoppo Hisatsugu Wakabayashi I. Normalina I. Nur Iqbal Kurniawinata, Mohamad Irzal Effendi Isni Rahmatika Sari Jasmanindar, Yudiana Jeanni Indah Noermala Julie Ekasari K. Sumantadinata Khairun Nissa Komar Sumantadinata Kukuh Nirmala L. Jamal Lastriliah, Mira M. Yuhana M. Zairin Junior M.S. Arifin Mia Setiawati Mochamad Fatuchri Sukadi Muchtar, Muthahharah Muh. Aris Muh. Fatuhcri Sukadi Muharram Nur Ikhsan Mulyani, Rahma MUNTI YUHANA Muttaqin, Helmy Faisal N.A. Maswan Nasri Julaini Nasrullah, Hasan Nur Bambang Priyo Utomo Nuzullia, Laely Odang Carman Ode, Inem P. Hadi Pras, Eva Prasetiyono Pratiwi, Kiki Amalia Putri, Shofii Amaliah R.D. Soejoedono Rafsyanzani, Muhammad Mufthi Rahman Rahman Ramadhani, Dian Eka Ramhirez, Putri Shandra Retno Damayanti Soejoedono Ririn Nurul Fauziah, Ririn Nurul Rizki Praseto, Rizki Rr. Bellya Anasti Maharani Rudi, Mad Ruku Ratu Borut Samsu Adi Rahman Sari Anggraeni, Sukma Septiani, Ghita Ryan Sri Nuryati Sri Nuryati Sugeng Budiharsono Sugiyo Hadi Pranoto Tatag Budiardi Trian Rizky Febriansyah Tsani Untsa, Agista Uba Umbara Ulil Surtia Zulpratita Umbara, Uba Wesly Pasaribu WIDANARNI WIDANARNI Wisriati Lasima Y. Tri Anggoro Yan Evan Yumaidawati, Nurfitriani Siti Yuni Puji Hastuti Zulfani, Anisa